Upload File

quan%ficaon)of)1,2)propylene)glycol,) …quan%ficaon)of)1,2)propylene)glycol,)...

  • Home
  • Documents
  • Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)
14
Quan%fica%on of 1,2 Propylene glycol, Glycerol and Sorbitol in Tobacco Products Dr. N.Palani, Devaraj Sathish Kumar, Deepa Pillai, T.K.Dinesh ITC R&D Centre, Peenya, Bangalore, India 2011_TSRC21_Palani.pdf TSRC2011(65) - Document not peer-reviewed

Upload: others

Post on 06-Jul-2020

9 views

Category:

Documents


0 download

Report

TRANSCRIPT

Page 1: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

Quan%fica%on  of  1,2  Propylene  glycol,  Glycerol  and  Sorbitol  in    Tobacco  Products  

Dr.  N.Palani,  Devaraj  Sathish  Kumar,  Deepa  Pillai,  T.K.Dinesh  

ITC  R&D  Centre,  Peenya,  Bangalore,  India  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 2: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

OBJECTIVE  

•    Propylene  glycol,  Glycerol  and  Sorbitol  are    humectants.    •   Humectants  have  been  used  in  tobacco  and  tobacco  products          to  maintain  the  moisture  level.  •   Humectants  are  used  in  the  range  of    0.5  –  5  %  w/w  in  tobacco  and          tobacco  Products.  

INTRODUCTION  

.

To  develop  a  single  method  for  analysis  of  humectants  (Propylene  glycol    Glycerol  and  Sorbitol)  in  tobacco  products  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 3: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

NEED  FOR  NEW  METHOD  

All  the  literature  methods  are  for  tobacco  and  cigare]es    and  no  methods    reported  for  snus.  

 The  matrix  in  snus  interferes  with  the  other  analytes  and  hence  making    quan%fica%on  difficult  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 4: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

Method                    Reference   Limita%ons  

A  AOAC  Official  Methods  of  Analysis  (1995),  Ch  3,  p.3   •   Only  Propylene  glycol  and  Glycerol  can  be  analyzed.  

B Beitrage  Zur  Tabakforschung.  Band  6  .  Heh  2.  1971  

•   Sorbitol  analyzed  separately.  •   Laborious  extrac%on  process  

C Journal  of  Chromatography,  140  304-­‐309  (1977)  

•   Pyridine  used  for  extrac%on.  •   Only  Propylene  glycol  and  Glycerol  can  be  analyzed.  

D CORESTA  Recommended  method  No  61   •   Separa%on  of  Glycerol,  Propylene  glycol          and  Sorbitol.  

METHODS  REPORTED  IN  THE  LITERATURE  

ALL  THE  ABOVE  METHODS  ARE  NOT  USED  FOR  ANALYSIS  OF  HUMECTANTS  IN  SNUS  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 5: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

PRINCIPLE  OF  THE  METHOD  

The  humectants  (Propylene  glycol,  Glycerol  &  Sorbitol)  are  converted  into  their  trimethylsilyl  ether  deriva%ves  and  these  silylethers  have  less  polarity,  making  them    more  vola%le  compounds.  

1H NMR OF1,2 - PROPYLENE GLYCOL, GLYCEROL & SORBITOL SILYL ETHER

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 6: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

FLOW  DIAGRAM  

0.5g Sample (30-50% Moisture)

Add 1ml Hexamethyldisilazane and 1ml Chlorotrimethyl silane

Add 5ml Hexane and Centrifuge for 20 min

Add 1ml DMF and vortex for 5 min

Vortex for 5 min

Take the supernatant layer and inject

in GC-FID

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 7: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

Column : DB-5 Dimension : 30 m X 0.25 mm X 0.25 micron Flow : 1.0 ml/min Injector Temperature : 250° C Detector Temperature: 280° C (FID) Oven : Initial 60° C hold 3 min Ramp1 increase @ 10° C per min to 280° C hold for 3 min Split ratio : 100:1 Injection volume : 1 µl Run Time : 30 minutes

GC-­‐FID  METHOD  CONDITION  

STANDARD CHROMATOGRAM

SPIKE SAMPLE CHROMATOGRAM GC-FID SNUS SAMPLE CHROMATOGRAM

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 8: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

LINEARITY  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 9: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

REPEATABILITY  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 10: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

REPRODUCIBILITY  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 11: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

RECOVERY  STUDIES  

LOW  SPIKE  RECOVERY:  SPIKED  AMOUNT  1%   HIGH  SPIKE  RECOVERY:  SPIKED  AMOUNT  2%  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 12: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

Glycerol Silylether

Sorbitol Silylether

PG Silylether

GC-­‐MS  MIX  STANDARD  CHROMATOGRAM  

PG Silylether

GC-­‐MS  SPIKE  SAMPLE  CHROMATOGRAM  

GC-­‐MS  CONFIRMATION  

Glycerol Silylether

Glycerol Silylether Sorbitol Silylether

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 13: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

ADVANTAGE  OF  OUR  METHOD  

•  Single  step  silyla%on  for  humectants  

•   Silyla%on  of  humectants      not  involving    use  of  pyridine    

•   Method  is  very  simple    and  requires  only  1  hr    for          complete  analysis  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed

Page 14: Quan%ficaon)of)1,2)Propylene)glycol,) …Quan%ficaon)of)1,2)Propylene)glycol,) Glycerol)and)Sorbitol)in))Tobacco)Products) Dr.)N.Palani,)Devaraj)Sathish)Kumar,)DeepaPillai,) T.K.Dinesh)

THANKYOU  

2011

_TS

RC

21_P

alan

i.pdf

TS

RC

2011

(65)

- D

ocum

ent n

ot p

eer-

revi

ewed


A novel approach for the synthesis of propylene glycol and ... · A novel approach for the synthesis of propylene glycol and ethylene glycol from glycerol using Raney Ni as a versatile

New Hexa Propylene Glycol Cyclotiphosphazene As Efficient ... · PDF fileNew Hexa Propylene Glycol Cyclotiphosphazene As Efficient Organic Inhibitor of Carbon Steel Corrosion in Hydrochloric

PROPYLENE GLYCOL PHENYL ETHER CAS N° - INCHEM

Pra Perancangan Pabrik Kimia Propylene Glycol Kapasitas 25

Formaldehyde. Propylene Glycol (Antifreeze) Cyanide, Arsenic

CSTR Design for Propylene Glycol Chemical Production

Propylene Glycol Ethers - IPCS INCHEM

Economic Impact Analysis for the Proposed Polyether ...€¦ · ethylene oxide, propylene oxide, or tetrahydrofuran. The initiator may be water, propylene glycol, ethylene glycol,

Viscosity Section 4.3 isopropanol vs. propylene glycol vs glycerol

PRODUCTION OF PROPYLENE OXIDE FROM PROPYLENE GLYCOL

EPA Glycol Document (PDF) - United States Environmental ... · and triethylene glycol. This category does not contain glycol ethers based on propylene glycol, dipropylene glycol,

Ingredients Purified Water, Glycerin, Propylene Glycol

The NTP-CERHR Monograph on Propylene Glycol - National

AD-A023 770 EFFECTS OF PROPYLENE GLYCOL 1,2-DINITRATE …

Glycol Heat-Transfer Fluids Ethylene Glycol versus ...Glycol.pdf · Glycol Heat-Transfer Fluids Ethylene Glycol versus Propylene Glycol Water is probably the most efficent heat-transfer

Propylene Glycol Ethers Methodology - Amazon S3 · 2019-05-24 · Rationale for propylene glycol ethers methodology All ICIS-published spot assessments in the weekly propylene glycol

TOXICOLOGICAL PROFILE FOR PROPYLENE GLYCOL - ATSDR

Computer simulation of the propylene glycol production

 · Web viewEthylene glycol and propylene glycol are used in manufacturing medicines, fuels, surfactants, antifreeze, lubricants, and solvents (Yue et al., 2012). Propylene glycol

Propylene oxide/Propylene glycols sector group...Propylene Oxide /Propylene Glycols Sector Group Guidelines for Handling and Distribution of Propylene Glycol USP/EP Revision 2 - March

Amended Safety Assessment of PEG Propylene Glycol Esters as

Ethylene Glycol and Propylene Glycol Toxicity

PROPYLENE GLYCOL (INTERCOOL P-300) · PROPYLENE GLYCOL (INTERCOOL P-300) ... environmental concerns and in areas where propylene glycol use is demanded by law. The inhibitors also

The effect of propylene glycol and polyethylene glycol 400

Pra Perancangan Pabrik Propylene Glycol dengan Bahan Baku Gliserol

Evolution Chemicals Propylene Glycol Information... · 2019-03-06 · • Evolution Chemicals Propylene Glycol reduces dependency on petroleum feedstocks, which improves our global

Batch Manufacture of Propylene Glycol - Penn Engineeringdlewin/CACHE_Workshop/Batch Manufacture of... · Batch Manufacture of Propylene Glycol by ... Recover the propylene glycol

Background review for the excipient propylene glycol · Background review for the excipient propylene glycol EMA/CHMP/334655/2013 Page 4/96 . Introduction. Propylene glycol is commonly

Propylene Glycol - Welcome to National Refrigerants Website

Propylene glycol (SIDS)

폴리염화비닐의 친환경 가소제로서 Poly(1,2-propylene glycol adipate) · 2015-06-05 · Poly(1,2-propylene glycol adipate) as an Environmentally Friendly Plasticizer

PROPYLENE GLYCOL Livestock - Agricultural Marketing Service

Catalytic Conversion of Glycerol to Propylene Glycol

Ivermectin (with Propylene Glycol) Formula- tion

Glycerol to Propylene Glycol - University of Pennsylvania