puriFlash® PurificationLow cost automated flash purification to

high-end flash/ preparative hybrid systems

50 bar 250ml/ min35 bar 250ml/ min30 bar 200ml/ min10 bar 150ml/ min 10 bar 800ml/ min

Pre-column holder Integrated column holder Extension kit for large columns

Flash columns up to 1.6KgLC Prep columns up to 80mm id.

Load & GoTM

an automated injection valve with loop for liquid or solid injection and column equilibration

InjectionMulti-mode liquid & solid

DetectionUV: 200 - 600 dual wavelength collectionFlow cell optical length 0.3mmSecond detector channel

PumpPatented Dual piston optimal solvent deliveryFlow rate: 250ml/ minPressure: 50 bar (max)Quaternary gradientAir purgeWashing disc

Unit control10.5” Touch Screen

USB: 4 portsRJ45

Flexible keyboard

Software (v.5.)Groundbreaking control and user friendly

Fraction collector4 Racks (short)

puriFlash® 450

May 2011

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puriFlash® - Enhanced Purification

The puriFlash® 2011 rangePF-210 - Robust entry level modular flash system PF-430 - Modular unit with enhanced flash/ prep featuresSPOT II - Classic chassis with flash/ prep capabilitiesPF-450 - Modular flagship system with optimal flash / preparative engineeringPF-800 - Enhanced flow rates to facilitate 800ml per minute

The comprehensive range of automated flash purification equipment provides a complete solution to the varied requirements of the chemistry and multifunctional laboratory.

PF-210 PF430 SPOT II PF-450 PF-800PumpFlow rate 150ml/ min 200ml/ min 250ml/ min 250ml/ min 800ml/ minPressure Max (bar). 10 30 35 50 10Gradient Binary Quaternary Quaternary Quaternary BinaryAir purge Option Option Yes Yes NoWashing disc No Yes No Yes YesDetectionUV (nm) 254 (default) 200-600 200-600 200-600 200-600Flow cell optical length (mm) 0.3 0.3 1.3 0.3 0.3Second detector channel Yes Yes Yes Yes YesInjectionDirect (fixed holder) Option Yes Yes Yes Yes6-way electrical valve Option Option No Yes OptionInjection mode: Liquid - Dry load Yes Yes Yes Yes YesLarge injection volume by external pump Yes Yes Yes Yes YesColumn HolderIntegrated Option Yes Yes Yes YesStand alone w/ 4-way valve Option Option Option No NoPre-column holder Option Option Option Yes OptionExtension kit for large columns Option Option Option Yes YesUnit ControlTouch screen (10.5”) Yes Yes Yes Yes YesUSB 2 4 4 4 4RJ45/ RJ11 Yes Yes Yes Yes YesFlexible keyboard Option Yes No Yes YesSoftwarepuriFlash soft (ver 5.0) Yes Yes Yes Yes YesFraction CollectorRacks 4 racks- short 4 racks- short 3 racks 4 racks- short 4 racks- shortSolvent TrayTray Option Yes Yes Yes Yes

puriFlash® 45050 bar 250ml/ min

puriFlash® SPOT II35 bar 250ml/ min

puriFlash® 43030 bar 200ml/ min

puriFlash® 21010 bar 150ml/ min

puriFlash® 80010 bar 800ml/ min

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puriFlash® multifunctionalityThe higher pressure puriFlash® range has been utilised worldwide for diverse and problematic purification applications that standard flash systems are unable to address.

Interchim’s high pressure units allow for a simple switching from a standard flash cartridge through to reduced micron, high pressure high speed cartridge and ultimately to stainless steel semi-preparative & preparative HPLC columns.

This capability creates a system that serves a laboratory’s or department’s multifunctional demand - from routine, day to day, clean-up procedures to more complex purifications as experienced by a number of our clients with crude mixtures or enantiomers.

Capital equipment that can serve a diverse range of projected and unforseen applications provides organisations with extensive cost savings for potential multiple system purchases and flexibility within their purification capability.

Flash to preparative transposition (Univ Munich)30 micron 40 Gram flash transposed to 10 micron prep LC (250 x 10mm)

The entry level modular puriFlash® 210 supports classic demand for mainstream automated purification. puriflash® 430 & 450 modular systems have 30 & 50 bar pressure capability respectively, delivered by a dual piston pump that provides unparalleled accuracy and robustness for solvent delivery in the flash environment. SPOT II utilizes the same patented pump within a classic chassis. These 3 systems form the heart of new system capability providing enhanced flash purification through cartridge silica/ media developments and semi-preparative and preparative applications. puriFlash® 800 delivers flow rates up to 800ml / min through modifications to the pump and solvent mixing chamber.

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Interchim’s patented pump is at the heart of the purification process in the higher pressure systems.

puriFlash® 430, 450 and SPOT II systems deliver unparalleled accuracy in the flash environment. The dual piston patented pump offers true low volume flow rates to 1ml / min within an accuracy of +/- 1% and up to 50 bar pressures at 250ml/ min flow rates in the 450 system.

puriFlash® Pump

puriFlash® Cartridge Holder

Dual piston pumps facilitate:

• greater accuracy, reproducibility and reliability.

• a constant flow rate whatever the system pressure.

• no need to place solvent bottles above the pump

True quaternary gradient(5 independent valves)

No cross contamination of solvents

High pressure mixing chambersmooth gradient variations

The puriFlash® range carries an integrated cartridge holder which holds flash cartridges from 4 -to- 330 gram sizes (optional on a 210 system).

An optional extension kit is available for larger cartridges (supplied as standard on the 450 system).

The puriFlash® 450 is also supplied with a pre-column holder for a dry or pre-load cartridge and the Interchim Load & GoTM automated loop injection valve which manages liquid and solid injection with column equilibration.

Suction tubing

Delivery tubingFitting

Plug

Check valve housing

Tee union

Bulkhead union

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puriFlash® SoftwarepuriFlash® version 5 software provides the chemist with the easiest interface yet for purification management. Users can create a method and control the purification process up to report generation, from a single screen.

puriFlash® Fraction Collector

puriFlash® Detection: UV/ ELSD

Tube Size mm No of tubes Tube Vol. ml Total Vol. ml

13 x 100 240 9 2160

16 x 150 176 22 3872

18 x 150 176 25 4400

18 x 180 176 32 5632

21 x 150 108 35 3780

25 x 150 64 60 3840

28 x 150 56 75 4200

29.5 x 200 56 110 6160

250 ml 3 x 2 250 1500

500 ml 3 x 2 500 3000

* Configuration not relative to SPOT II** 18 x 150mm racks standard

Key/ Unique features:

• Interactive & customisable user interface

• Define report format parameters

• TLC to flash transposition• Auto gradient optimisation• Intelligent continuous mode• Dual wavelength triggered

collection• Timed auto shut-off• Air Purge

The puriFlash® fraction collector for the modular 210, 430 and 450 systems benefits from 4 front loading racks that maximise sample volume collection, flexibility and ease of use.

UV detection is supplied as standard with all Interchim systems. The entry level 210 system is supplied with a fixed wavelength (254nm std). The rest of the range is supplied with an adjustable 200 - 600nm wavelength with dual wavelength collection. This can be increased to 840nm as an option. Flow cell optimal length is 0.3mm (1.3mm for SPOT II).

Interchim optional ELSD is exclusively optimised to flash applications and offers key features:

• Flash specific nebulizer to avoid clogging from flash grade solvents• Signal optimisation to avoid saturization from flash sample loading • Operational through version 5 software (mid-summer 2011)

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puriFlash® CartridgesThe comprehensive puriFlash® cartridge range comprise of various silica and specialty phases for flashpurificationwithinarangeofcartridgesfrom4g to 1.6KG.

puriFlash® Silica, Polymeric & Specialty CartridgesItem Functionality

Silica Non-ionic, polar organic compoundsIR Standard Irregular Silica, Non-ionic, polar organic compoundsHP High Performance Silica, Non-ionic, polar organic compoundsHC High Capacity Silica, Non-ionic, polar organic compoundsC18 ApplicationssuitedtoC18chemistryandfullendcappingC18T LowcarbonC18formatssuitedtomildlyhydrophobicpeptides/oligopeptidesC8 Mildhydrophobicpeptides/oligopeptidesC4 Hydrophobicpeptides/oligopeptidesSCX Cationicchargedcompounds,basicorganiccompoundsDiol PolaritybetweenC18&Silicaoffersselectivityvariation.StrongsolventcompatibilityCN Boronic acidsDEAP Diethylaminopropyl - Less polar form of NH2 for med to high polarity compoundsMM1 Mixedmode-Reversephase&cationexchange.Mid/nonpolarorganiccompoundsNH2 Carbohydrates, polar organic compounds, anion and organic acidsNH2 HC Increased capacity for Carbohydrates, polar organic cmpds, anion & organic acidsActivatedCarbon Forpurification,decolourizationandremovalofunwantedby-productsPolymeric (302H) Mixedpolymerwithgoodselectivityforacidic,basicandneutralcompoundsinRFPolymeric (Atoll) APSDVBPolymer-alternativetoC18selectivityPolyamide Flavonoids, Natural plant extractAlumina Non-Ionic polar compounds

Proprietary packing processes ensure the mostefficienttransferofsampleacrossthe sorbent bed (polypropylene housing guaranteesnoplasticleaching).

Cartridges have been comprehensively designed and tested to withstand up to 22 bars of pressure for the most commonly utilisedcartridgesizesand20barsupto800gram units. Silica based supports are typically available in15μm,30μmand50μm.

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puriFlash® Cellulitic CartridgesItem Functionality

DEAE Weakanionexchange.Proteins,peptides&enzymesQA Stronganionexchange.Proteins,peptides&enzymesCM WeakCationexchange.Proteins,peptides&enzymesSulfate Concentration,purification&depyrogenationofvirus,viral/microbialantigens&

heparin-binding proteinsPhenyl HydrophobicinteractionButyl HydrophobicinteractionEndotoxin Immobilizedpoly(ε-lysine)Cellufine™beadsforendotoxinremovalfromsolution

puriFlash® Dry load Cartridges

puriFlash® CellufineTM Cellulitic CartridgesCellufine™isachromatographymediamadefromsphericalcelluloseparticlesdisplayinghighchemicalstability,highmechanicalstrengthandbio-compatibility.Itwasdesigned&isparticularlysuitedforthepurificationofproteins,enzymesandotherbiomolecules.Cellufine™suffersmuchlessmatrixleakingthansyntheticpolymermedia and is ISO 9001 (2000) registered.

Cellufine™puriFlash®cartridgesfacilitatehigherbiologicalsamplethroughputsandhavebeenutilisedwiththepuriFlash® purificationsystems.

puriFlash® Dry load CartridgesSize Pressure

(bar)Type

4 gram 10 Empty 50% HC silica 80% HC silica 80% Celite 80% C18 silica 50% C18 silica

12 gram 15 Empty 50% HC silica 80% HC silica 80% Celite 80% C18 silica 50% C18 silica

25 gram 15 Empty 50% HC silica 80% HC silica 80% Celite 80% C18 silica 50% C18 silica

40 gram 15 Empty 50% HC Silica 80% HC silica 80% Celite 80% C18 silica 50% C18 silica

PuriFlash®Dryloadcartridgesarescrewcapflashcartridgesthathavebeendesignedfortheloadingofinsolublesamplesontoyourpurificationcartridge.

Cartridges are available empty for you to load your own silica, celite and sample;orprefilledwithCelite(fornon-adsoptiveloading),C18SilicaorHCsilicaformaximumadsorption.

PuriFlash® Dry load cartridges have also been used as a pre-load cartridge for soluble samples.

CHESHIRE SCIENCES (UK) LTD.67 Bridge Street Rows,Chester, Cheshire CH1 1NW

Tel: (01244) 620486Fax: (01244) 620378

E-mail: ask@cheshiresciences.comwww.cheshiresciences.com

Selected applications

• Ultra performance flash purification for improved speed and resolution

• Utilization of 15 micron silica cartridges for purification of diastereomers

• A purification approach for crude reaction mixtures

• Rapid Guaifenesin enantiomer purification

• Single system transposition from flash cartridge purification to preparative HPLC column purification

puriFlash® systems have been utilised in the field for a variety of applications generally not associated with a flash system. Further information on these (and other) applications are available through discussion with one of our product specialists.