pseudomonas and related organisms aerobic nonfermenters pseudomonas aeruginosa : opportunistic...
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Pseudomonas and related organisms
Aerobic nonfermenters
Pseudomonas aeruginosa: opportunistic infections of multiple sites
Burkholderia cepacia: RT infection in cystic fibrosis patients, UTI, opportunistic infections
Burkholderia pseudomallei: opportunistic pulmonary infections
Stenotrophomonas maltophilia: opportunistic infections
Acinetobacter baumannii: opportunistic infections of RT
Moraxella catarrhalis: opportunistic RT infections
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Pseudomonas
Structure and Physiology
Gram-negative rods.
Motile with polar flagella.
Obligate aerobe.
Oxidase-positive.
Do not ferment carbohydrates.
Resistant to multiple drugs.
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P. aeruginosa
Forms round colonies with a fluorescent greenish color, sweet odor, and -hemolysis.
Pyocyanin- nonfluorescent bluish pigment;
pyoverdin- fluorescent greenish pigment;
pyorubin, and pyomelanin
Some strains have a polysaccharide capsule.
Identification of P. aeruginosa is usually based on colonial m
orphology, -hemolysis, oxidase positivity, the presence of c
haracteristic pigments and sweet odor, and growth at 42 oC.
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P. aeruginosaPathogenesis and Immunity
This organism is widely distributed in nature and is commonly present in moist environments in hospitals. It is pathogenic only when introduced into areas devoid of normal defenses, e.g.,
1. Disruption of mucous membrane and skin.
2. Usage of intravenous or urinary catheters.
3. Neutropenia (as in cancer therapy).
P. aeruginosa can infect almost any external site or organ.
P. aeruginosa is invasive and toxigenic. It attaches to and colonizes the mucous membrane or skin, invade locally, and produces systemic diseases and septicemia.
P. aeruginosa is resistant to many antibiotics. It becomes dominant when more susceptible bacteria of the normal flora are suppressed.
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P. aeruginosa
Antigenic structure, enzymes, and toxins
Pili and nonpilus adhesins.
Polysaccharide capsules (alginate, glycocalyx): seen in cultures from patients with cystic fibrosis.
LPS- endotoxin, multiple immunotypes.
Pyocyanin: catalyzes production of toxic forms of oxygen that cause tissue damage.
Elastases: helps bacteria spread and inhibit neutrophil chemotaxis; induces antibodies in chronic infections.
Hemolysins
Phospholipase C;
Rhamnolipid, also inhibits ciliary activity.
Exotoxin A: causes tissue necrosis and is lethal for animals (blocks protein synthesis); immunosuppressive.
Exoenzyme S and T: cytotoxic to host cells.
Pathogenesis and Immunity
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P. aeruginosa
Infection of wounds and burns (blue-green pus).
*Verdoglobin or fluorescent pigment detected in wounds, burns, or urine by UV fluorescence.
Skin and nail infections
Meningitis (when introduced by lumbar puncture).
Pulmonary infection
Necrotizing pneumonia in CF patients (diffuse, bilateral bronchopneumonia with microabscess and necrosis).
Eye infections: corneal ulcer.
Ear infections
Otitis externa: mild in swimmers; malignant (invasive) in diabetic patients.
Chronic otitis media
Endocarditis seen in intravenous drug abusers.
Urinary tract infection
Sepsis: most cases originate from infections of lower RT, UT, and skin and soft tissue.
*Ecthyma gangrenosum in sepsis: hemorrhagic necrosis of skin, often do not contain pus.
Clinical Diseases
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Laboratory Diagnosis
Specimen: skin lesions, pus, urine, blood, spinal fluid, sputum.
Culture: blood agar plate and differential media. Identification of P. aeruginosa is described above.
Several subtyping methods, including phage typing and molecular typing, are available for epidemiologic purposes.
Treatment
Combined antibiotic therapy is generally required to avoid resistance that develops rapidly when single drugs are employed. Avoid using inappropriate broad-spectrum antibiotics, which can suppress the normal flora and permit overgrowth of resistant pseudomonads.
Injection of hyperimmune globulin and granulocyte transfusion to augment compromised immune function for selected patients.
P. aeruginosa
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P. aeruginosaPrevention and Control
Pseudomonas spp. normally inhabit soil, water, and vegetation and can be isolated from the skin, throat, and stool of healthy persons.
Spread is from patient to patient via contact with fomites or by ingestion of contaminated food and water.
Methods for control of infection are similar to those for other nosocomial pathogens.
Special attention should be paid to sinks, water baths, showers, hot tubs, and other wet areas.
High risk population: patients with leukemia, burns, cystic fibrosis, and immunosuppression.
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P. aeruginosa
Prevention and Control
Control:
1. Patients at high risk should not be admitted to a ward
where cases of pseudomonas infection are present.
2. Patients infected with P. aeruginosa should be
isolated.
3. Sterilize
all instruments, apparatus, and dressing;
antimicrobial and other therapeutic substances.
4. Monitor clinically relevant isolates of P. aeruginosa by
a suitable typing system to identify epidemic strains.
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Stenotrophomonas maltophiliaA common nonfermentative, gram-negative isolate.
It infects debilitated or immunocompromised persons,
and causes a wide spectrum of diseases, including wo
und infections, UT infections, pneumonia, sepsis, men
ingitis, etc.
It is resistant to many commonly used antibiotics, and
patients receiving long-term antibiotic therapy are parti
cularly at risk for acquiring infections.
Infections may be acquired from contaminated disinfe
ctants, respiratory therapy and monitoring equipment,
and ice machines.
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BurkholderiaThey colonize the moist environmental surfaces and are co
mmonly associated with nosocomial infections.
B. cepacia and B. pseudomallei are important pathogens.
B. cepacia causes RT infections particularly in cystic fibrosis
patients, UT infections and septicemia. Usually non-fatal exc
ept for RT infections in CF patients.
B. pseudomallei usually causes opportunistic infections, but
may sometimes infect previously healthy persons. Infection
by this organism may result in asymptomatic infection, acute
suppurative cutaneous infection that may progress to sepsis,
and chronic pulmonary infection ranging in severity from mil
d bronchitis to necrotizing pneumonia.
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2005/7/30 台南高雄疑似發生類鼻疽疫情,疾病管制局提醒民眾,皮膚如有傷口,請勿接觸污染的土壤或水源
疾病管制局今天公佈今年自七月 11 日至 29 日以來,類鼻疽累計通報 16 例,其中高雄縣 9 例、台南市 4 例、高雄市 2 例、台南縣 1 例。其中 6 例死亡, 3 例在加護病房,另 7 例住普通病房。類鼻疽係由類鼻疽伯克氏菌 Burkholderia pseudomallei 所造成的臨床感染症,屬假單孢菌屬革蘭氏陰性桿菌,此菌在土壤、水池及積水環境中存在,會感染馬、羊、豬等動物以及人類。其流行地域為東南亞地區及澳洲北部的熱帶地域。該局自 89 年即將此病納入監測。 89 年通報病例 1例、 90 年 15 例、 91 年 9例、92 年 5例、 93 年 13 例。本次疫情發生原因,疾病管制局初步調查研判可能係因日前南部豪大雨,將土壤中之病菌沖刷出來,所造成的民眾感染事件,病例多發生在二仁溪流域。該局鄭重呼籲在二仁溪流域附近居民,若有發燒等症狀者,務必迅速就醫。並告訴醫師居住地區,疾病管制局呼籲,醫師對於上述地區發燒病患,應先排除感染此病的可能性,若有懷疑應立即以抗生素治療,並採檢送驗。