provider of global contract research services accelerating preclinical research, drug discovery...
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Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
Benefits of Xenograft Develop new therapeutic agents quickly,
efficiently and cost effectively
Evaluate the efficacy and toxicity of potential therapeutic agents
Evaluate target compound activity using in vivo system (human cells)
Predict cytotoxicity of cancer drugs
Xenotransplantation is the transplantation of living cells, tissues or organs from one species to another.
Services > Xenograft
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Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
In vivo xenograft efficacy evaluations remain the gold standard and the workhorse of oncology preclinical research.
Xenograft models combine the advantages of working with human cancers with the physiological relevance of an in vivo host.
Typically, immunodeficient mice serve as hosts for a wide variety of human tumors, effectively serving as proxies for human subjects.
Xenograft models remain a mainstay in oncology research, playing a key role in drug discovery and development.
The Gold Standard in Oncology
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Pipeline Xenograft Assay I We design your experiment based on your project requirements. Through years of experience and literature search we guarantee optimal experimental setup according to selection of mice stain, cell lines, inoculation and dosage.
Pipeline Assay II - test a compound You send us a compound and cell line to establish xenograft. We establish xenograft animal models and test compound activity and how it affects tumor growth in an established xenograft model.
Pipeline Xenograft Assay III - test a cell line You send us a cell line and we test it’s ability to establish tumor growth in standard immunodeficient mice.
Assay Pipelines
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
How does the test compound affect tumor growth? Does the test compound inhibit tumor invasion or metastasis? Localization of test compound in the tumor? Define drug characteristics:
Bioavailability Toxicity Efficacy Potency
Is cell line tumorigenic? Changes in gene expression upon treatment or gene manipulation ParamHow does gene manipulation in a cell line affect following parameters:
Tumor growth , Invasiveness, Metastasis
Experimental Objectives of Compound Testing
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177 www.skyepharma.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Basic Study Design
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
Xenograft studies can be designed to identify lead compounds, optimize dose schedules, and identify combination strategies.
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Use in-house or customer-provided cell lines or tumor Begin testing a compound immediately after injecting mice with tumor cells
(unstaged) or once the tumors reach a certain size (staged) Assign mice to groups randomly or stratify them by body weight, tumor size,
disease factors, or any combination of parameters Administer test compounds by any of the following methods: topically, IP, IV,
SC, PO, high pressure tail vein, osmotic minipumps (SC or IP), SC drug pellets, or in the food or water
Snap freeze harvested tumors or fix them for histology or other specialized assays
Analyze blood chemistry at various times throughout the study
Service Options
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > Xenograft
Cell Line Organ Disease A549 Lung Carcinoma DU145 Prostate Carcinoma PC3 Prostate Adenocarcinoma LNCaP Prostate Carcinoma MDA-MB-231 Breast Adenocarcinoma FaDu Pharynx Squamous carcinomaCaki -1 Kidney Clear Cell Carcinoma Caki - 2 Kidney Clear Cell Carcinoma PANC-1 Pancreas Epithiloid Carcinoma SK-MEL-2 Skin Malignant Melanoma SK-OV-3 Ovary Adenocarcinoma U-87 MG Brain Glioblastoma/ Astrocytoma A431 Epidermis Epidermoid Carcinoma SW-480 Colon AdenocarcinomaHeLa Cervix Adenocarcinoma
Sample of Cell Lines Maintained at Altogen Labs
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Growth rates for different cell linesServices > Xenograft
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Route of dose: Intratumoral Intramuscular Oral Intravenous Intratracheal Subcutaneous Intraperitoneal
Frequency of dose Duration of dose
Administration Options
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
www.patient-derived-xenograft-services.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Estimated tumor volume (L x W2 /2) Mean/median time to specified tumor volume Survival time to specified tumor volume Tumor doubling time Tumor growth inhibition (TGI) Tumor growth delay (TGD) Increased life span (ILS) Tumor cell kill rate
Endpoints
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Clinical observations every dayHaematology analysis (blood cell count)Clinical chemistry profile (of urine and plasma)Evaluate the degree of metastasis. Characterization of the tumor (necrosis, etc)The tumor is divided and preserved for further analysis Cryo-preservation (for cell culture) Formalin fixed tumors and tissues (for histopathological and
immunostaining analysis) RNAlater fixed tumors and tissues (for RNA and DNA
isolation, and gene expression analysis)
Additional Analysis Options
Services > Xenograft
Altogen Labs • 4020 S Industrial Dr • Suite 130 • Austin • TX • 78744 • [email protected] • 512.433.6177