prostar 210/215 and prepstar 218/sd-1 solvent delivery...

Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA

©Varian, Inc. 2005 03-914947-20:Rev. 5

ProStar 210/215 and PrepStar 218/SD-1 Solvent Delivery

Module Control

Galaxie Driver Manual

ProStar 210/215/ PrepStar 218/SD-1 SDM -2- 03-914947-20:5

Table of Contents Introduction.............................................................................................. 3

General ........................................................................................................................... 3 Installation and Configuration................................................................ 4

Installing the Driver Software .......................................................................................... 4 Configuring the Communication Server.......................................................................... 4 Configuring the Driver ..................................................................................................... 5

Configuring the ProStar / PrepStar Pump ................................................................... 5 Configuring the Synopsis ............................................................................................ 9

Building a Method ................................................................................. 12 Creating a New Method ................................................................................................ 12 ProStar / PrepStar Pump Control Method .................................................................... 14

Elution Control ........................................................................................................... 16 Miscellaneous Control ............................................................................................... 18 Pressure Control........................................................................................................ 19 Injection Control......................................................................................................... 20

Starting and Viewing an Acquisition.................................................... 22 Quick Start and Sequence ............................................................................................ 22

Quick Start (Single Acquisition)................................................................................. 22 Sequence .................................................................................................................. 23

Running Acquisition Status ........................................................................................... 24 Viewing a Running Acquisition .................................................................................. 24 Status of the ProStar / PrepStar Pump ..................................................................... 24

Appendix ................................................................................................ 30 Pressure Limits (in bar)................................................................................................. 30 Compressibility and Pressure Constant........................................................................ 31


Introduction

General This document describes the configuration of the Varian ProStar 210, 215 and Varian PrepStar 218, SD-1 pump driver. It also explains how to build a control method for this driver, how to start and view a running acquisition and what actions are available during an analysis.

The ProStar 210, 215 and PrepStar 218 Solvent Delivery Modules are identical in appearance and operation. The ProStar 215 and PrepStar 218 have more powerful motors, larger power supplies and can operate at higher pressures than the ProStar 210. The pressures are identified in the Appendix.

The manual will refer to the pumps as 210/215/218/SD-1 throughout.


Installation and Configuration

Installing the Driver Software To install the Galaxie Drivers software refer to the Galaxie Data System Installation Guide.

Configuring the Communication Server

To communicate with the ProStar 210-215-218-SD-1 module a RS232_Interface or a RS232_PC communication bus must be configured on the acquisition server.

The parameters of the bus to must be the following:

Stops

1 Stop

Baud 19200

Parity EVEN

Bits 8 Bits

Flow Control NONE

Buffer Tx 4096

Buffer Rx 4096

NOTE: For more details about the configuration of a RS232_Interface or a RS232_PC bus, refer to the Galaxie Chromatography Data System Installation’s Guide.


Configuring the Driver The driver software configuration is done in the Galaxie Configuration Manager when a new chromatographic system is created.

Configuring the ProStar / PrepStar Pump

Click on the button corresponding to the ProStar / PrepStar pump driver and the pump configuration screen appears:

The toolbar allows you to easily scroll through the configuration windows:

to select the first configuration window.

to select the previous configuration window.

to select the next configuration window.

to select the final configuration window.


Communications Window

Select from the list the name of the RS422 Bus where the system has been connected.

NOTE: For more details about the RS422 Bus configuration, refer to the section Configuring the Communication Server in this manual.

Selection will not appear until the RS422 BUS is configured.


Hardware Window

Select from the list the type of the pump: isocratic, binary, ternary, quaternary, isocratic + injector, binary + injector, ternary + injector. A new tab (Pump B, Pump C, Pump D or Injector) appears depending on the selected type of pump.

NOTE: - The system must be equipped by at least two ProStar / PrepStar modules to work as a binary pump, three modules for a ternary one and four to work in quaternary mode.

- With this type of pump, a module can be used as an injector. In this case a new Injection screen becomes active in the method. To learn more about this option, refer to the chapter Control method for the ProStar / PrepStar pump.

- When an Injector module is configured, in the lower part of the Galaxie Configuration Manager an injector (of the new system) called ProStar 210/215/218 pump is created.

The ID entered here must match the ID of the pump.


Enter the identification number of the pump module in the RS-422 unit ID field. The RS-422 unit ID field must match the ID specified on the pump itself. To check the ID on the pump, press Setup on the pump (no flow), scroll left until the ID is displayed. Press Enter when finished.

Select from the list the model of the pump module. Four choices are available: ProStar 210, 215, PrepStar 218 and SD-1.

The 210/215/218 modules are identical in appearance and operation. They differ only in one respect: the ProStar 215 and PrepStar 218 have more powerful motors, larger power supplies and can operate at higher pressures than the ProStar 210.

NOTE: The pump head size must be configured on the instrument. Maximum flow rates authorized in Galaxie will then depend on that configuration. - With ProStar 210/215 and PrepStar 218: press the ~ button to access the setup menu and enter the correct head size under Hdsz section using the ▲ and ▼ buttons. - With the PrepStar SD-1: press the ~ button to access the setup menu. Select YES under Change Pump using the ▲ and ▼ buttons and follow the procedure displayed in the LCD screen. At the end of the procedure, new pump head size will be automatically configured. Please refer to the instrument manual for more details.


Pressures Window

Select from the list the Pressure units. Three choices are available: bar, PSI and kPa.

Configuring the Synopsis In the Overview part of the system configuration, a synopsis can be defined for the system. This synopsis depends on the configured devices.

1. To save the configuration of these devices, click on the OK button.

2. Right click on the system name in the bottom part of the Galaxie Configuration Manager screen. A pop-up menu appears: click on Stop. Then right click on the system name again, and click on Start.


3. Go back to the System Configuration screen (Properties

wizard). A graphic of the devices that comprise the system is displayed in the synopsis screen. To configure the synopsis as the real instrument configuration, move each module, and then connect it appropriately. To do this, select one anchor for each of the two modules (the selected anchors become

red) and click on the button; a connection tube will appear between the two anchors.

Click on the button to delete a selected tube, click on

the button to delete all the tubes.

Click on to define a background color, or on to make the background transparent. Click on the icon to change the grid color.

4. Once the synopsis configuration is correctly done, it will be displayed in Galaxie Chromatography Data System, in the Systems tab. In this tab, the Overview part will show the synopsis of the system with some parameters displayed (temperature, flow, signal, etc.) depending on the installed devices.

For example, the following pictures represent the synopsis of the ProStar / PrepStar pump:


Galaxie Configuration Manager Screen

Galaxie Chromatography Data System Screen

NOTE: Once the system has been created and correctly configured, it must be associated with one or more projects in order to start acquisitions. For more details about the creation and configuration of a system, refer to the Galaxie Configuration Manager User’s Guide (chapter The System).

Click on the button to print the current configuration


Building a Method

Creating a New Method The method contains parameters for instrument control, data acquisition, chromatogram processing, and editing of final results. To initially set up a method, it is not necessary to define all of the sub-method sections; however, you must fill in the instrument control section prior to starting an acquisition.

1. To create a new method, select the FILE / NEW METHOD option from the Galaxie Chromatography Data System main menu.

A wizard guide is displayed to provide assistance during the first steps of method creation:

2. Choose the system (chromatograph) associated with the

method. A method is created for a particular system. When


starting an acquisition, the name of the system that performs the acquisition must be correct, since method access is limited only to those methods associated with this system.

3. Once this field is completed click on the Next button to move to the second step of the method creation:

4. Enter the New Method Name in the first field. In the

Description field, enter information concerning the method (optional).

5. Once these two fields have been completed, click on the OK button. The new method is created and is opened with default parameters in the Galaxie Chromatography Data System. The next step is the definition of each of the method sections.

The instrument control section must be defined in order to start an acquisition. In the following part of this guide, only the instrument control section of the method for the ProStar / PrepStar pump is detailed. Other parameters such as Injection Volume, Vial and Rack number could be necessary. They are defined in the acquisition sub-method part.

NOTE: For more details about the programming of each method section, refer to the Galaxie Chromatography Data System User’s Guide.


ProStar / PrepStar Pump Control Method Once the new method is created, select the control section in the lower part of the browser.

The following screen appears:


Press the button to display the System Control Method Advanced Tools window:

to send the current method to the instrument (not applicable to this driver)

to get the active method from the instrument (not applicable to this driver)

to print the current method

Click on the button to display the ProStar / PrepStar pump control method section. It contains up to four sub-sections: Elution, Miscellaneous, Pressure and Injection. Click on the required sub-section to define the corresponding parameters that will be used during the chromatographic run. The toolbar allows you to easily scroll through the control method windows:

to select the first window of the control method.

to move back to the previous window of the control method.

to move forward to the next window of the control method.

to select the final window of the control method.


Elution Control

Select from the list(s) the solvent of each pump module. Here are listed all the available solvents:

Water Acetone Acetonitrile Benzene Carbon tetrafluoride Chloroform Cyclohexane Dichloroethane Diethyl Ether Dimethylformamide Dioxane Ethanol Ethyl Acetate Methylene Chloride Methanol n-heptane n-hexane o-dichlorobenzene Propanol Tetrahydrofuran Toluene 2-Methylformamide


A compressibility factor and a pressure constant are associated with each solvent (Compressibility and Pressure Constant fields).

When the Compressibility compensation field is unchecked, the pump will not take into account the corresponding factors and the factors will be concealed.

Refer to the Compressibility and Pressure Constant section in the appendix of this manual to find the list of these values.

The refill time parameter is the total time that it takes to refill the pump head with mobile phase. This value may be changed when a very viscous mobile phase is used. In this case, a rapid refill might lead to cavitations or bubble formation in the pump head causing a lower than expected flow rate. If the refill time is too long, excessive pulsations in the flow may be observed. This parameter is programmable by pump module.

NOTE: The activation of the scrolling lists A, B…D depends on the type of the pump previously configured in the Galaxie Configuration Manager (isocratic, binary, ternary or quaternary pump).

Program a flow and/or a composition gradient. Each step of the program includes time, flow and/or composition new values. The Pumps Gradient Chart represents the evolution of these parameters.

Use the following buttons to edit the table:

to add a line to the table.

to delete a line in the table.

to clear the entire table.

Below the pump gradient chart, select the display type of the chart. Two choices are available Solvent A on top or Solvent A on bottom.


Miscellaneous Control

Start mode:

In the scrolling list, select the way to start the pump.

If immediately when ready is chosen, the pump will start as soon as it is ready.

If inject trigger on pump X is chosen, the pump will start when an inject marker is send to pump X.

End of Sequence:

In the scrolling list, select either to stop or not the pump at the end of the analysis (Quick Start or sequence).

If Leave Pump On is selected, the pump will continue to work at the last time-table programmed flow and composition.


Pressure Control

Pressure Limits Parameter Group:

Enter the minimum and maximum pressure limits of the pump. Outside these limits, the pump will stop and an alarm will sound.

NOTE:

- The maximum pressure limit of a ProStar / PrepStar module depends on the type of its pump head. This limit is automatically adapted according to the connected pump head. To know these limits, refer to the Pressure Limits chapter in the appendix of this manual.

- If a higher value of pressure is entered by error, a message appears indicating the pressure limits for the connected pump head and the last entered valid value is displayed.

- If several ProStar / PrepStar modules are connected (in case of binary, ternary or quaternary pump), the maximum pressure limit is the lowest value among the different pump ones.


Injection Control

This window becomes active only when a pump module is configured as an injector in the system (for more details refer to the Configuration of the ProStar / PrepStar pump chapter).

Thru Pump Injection Parameter Group:

Enter the time when the injection will start in the Injection time field.

Enter the flow rate of the injection in the Injection Rate field. The end time of the injection is determined according to the flow rate and the injection volume entered in the Acquisition method section (or in the Quick Start window or in the Sequence).

NOTE: The Injection Volume entered in the Acquisition part of the method must be entered in mL only.


NOTE: During the injection period, the current flow of each pump modules will be adjusted to take into account the injection flow as shown below.


Starting and Viewing an Acquisition

Quick Start and Sequence Once the instrument control method has been correctly programmed, an analysis can be started. The next section briefly describes how to start an acquisition.

Quick Start (Single Acquisition)

1. Press the button.

In the Quick Start window, select the system, the method, and optionally the project if connected in "All project" mode.

2. Press the OK button

The following screen is displayed:


In the Acquisition parameters tab, required fields like the Run Name, the Run ID and the Acquisition length must be filled in before starting an analysis. If the system is equipped with an autosampler controlled by the Galaxie Chromatography Data System, the Injection Volume, the Vial position and the Rack number (if required) must be entered.

Other parameters for each acquisition channel can be entered in the Channel X tab.

Click on the Start button to start the acquisition if the system is a single-injector system or click on the Next button if the system is a multiple injector system. In a multiple injector system, the same parameters as the ones entered for the first injector must be entered before starting the acquisition.

Sequence 1. To create a sequence, select the FILE / NEW SEQUENCE

menu, complete the displayed creation wizard screen (refer to Galaxie Chromatography Data System User’s Guide), by entering the system name that will perform the analysis, the


number of lines (corresponding to the number of samples) and the sequence name.

2. The sequence is displayed: complete the information sequence columns (refer to Galaxie Chromatography Data System User’s Guide).

The Method Name, the Run Name, the Run ID and the Acquisition length must be filled in before starting the analysis. All these fields are automatically imported from the acquisition part if defined in the method.

3. Start the sequence by clicking on the button.

NOTE: For more details about how to start an acquisition, refer to the Galaxie Chromatography Data System User’s Guide.

Running Acquisition Status

Viewing a Running Acquisition To view a running acquisition, click on the Systems tab then select the running system. A window is activated which is composed of two parts: the running chromatogram and the status window where the driver modules are listed.

Status of the ProStar / PrepStar Pump

Click on the button corresponding to the ProStar / PrepStar pump driver, and the pump status screens will appear. It contains four sub-sections: Elution, Alarm, About and Overview. Click on the required sub-section to display the corresponding parameters that will be used during the chromatographic run.


A toolbar allows you to easily scroll through the status windows:

to select the first status window.

to move back to the previous status window.

to move forward to the next status window.

to select the final status window.

Elution Window

In this window, the elution parameters (solvent composition, pressure, flow, etc.) are shown with their current values, depending on the control method program.

Most of these values can be modified during the acquisition or in order to start the pump for equilibration for example.

The , … buttons can be activated depending on the pump configuration previously done in the Galaxie Configuration Manager (isocratic, binary, ternary or quaternary pump).


Click on one of these buttons and the following screen appears:

The Flow and/or the solvent composition (%x) values can be set. The Ramp parameter is the time for each module to join linearly the new setting values. Click on OK to validate.

Click on the button and the same screen as above will be displayed.

Click on or to start or stop the pump.

Click on the button when the pump is running to stop the progression of the gradient at its current value.

Click on the button and the progression of the gradient starts again from its current value to the ramp end point.

NOTE: The chart shown on the right panel of the Elution window represents the progress of the flow and solvent composition.


Alarm Window

When the pressure is out of the limits, the pump stops automatically and the pump status can be read in this screen. The light becomes red indicating the nature of the error. It is possible to define new pressure limits directly from this status

screen, by clicking on the button.

The same screen than above appears:

Enter the new values of pressure limits. Outside these limits, the pump stops and the alarm sounds. Click on OK to validate.


About Window

This tab is a read-only screen, where the pump head type and the number of ProStar / PrepStar modules are shown.

Overview Window

Click on the button: the Status Overview window is displayed and contains three tabs:

• General: displays the synopsis of the instrument, where some current values can be read (flow, pressures, etc.).

In this window, some parameters are shown, like the current flow, the current pressure and the composition for each pump module.


The same actions than in the Elution window can be performed by clicking on the appropriate buttons.

• Not Ready: contains the not ready information sent by the instrument.

• Events: contains the events that have occurred on the instrument.


Appendix

Pressure Limits (in bar) ProStar 210 ProStar 215 PrepStar 218

Head

Flow Rate Range (mL/min) SS and Ti PEEK SS and Ti PEEK SS and Ti PEEK

5 0.01-5 599 NA 599 NA 599 NA

10 0.01-10 599 275 599 275 599 275

25 0.025-25 317 275 410 275 410 275

50 0.05-50 137 137 275 275 275 275

100 0.01-100 82 82 137 137 275 137

200 0.01-200 NA NA NA. NA. 241 NA


Compressibility and Pressure Constant Solvent Compressibility

(Mbar-1) Pressure constant (bar)

Water 46 3231

Acetone 128.9 956

Acetonitrile 97.4 1212

Benzene 96.7 1046

Carbon tetrachloride 106.7 998

Chloroform 97.4 1227

Cyclohexane 114 800

Dichloroethane 111.9 1020

Diethyl Ether 188 700

Dimethylformamide 80 1500

Dioxane 60 1500

Ethanol 115 1100

Ethyl Acetate 100 1800

Methylene Chloride 97.4 1212

Methanol 125 1200

n-heptane 144 760

n-hexane 167.2 644

o-dichlorobenzene 95 1400

Propanol 98 1200

Tetrahydrofuran 95 1500

Toluene 93 1200

2-Methylformamide 80 1500

ProStar 210/215 and PrepStar 218/SD-1 SDM Control Table of Contents Introduction General

Installation and Configuration Installing the Driver Software Configuring the Communication Server Configuring the Driver

Building a Method Creating a New Method ProStar / PrepStar Pump Control Method

Starting and Viewing an Acquisition Quick Start and Sequence Running Acquisition Status

Appendix Pressure Limits (in bar) Compressibility and Pressure Constant

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