prokaryotic profiles: the bacteria and the archaea
TRANSCRIPT
Prokaryotic Profiles: the Bacteria and the
Archaea
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Characteristics of Cells
• Eucaryotic cells: animals, plants, fungi, and protists– contain membrane-bound nucleus with DNA as
chromosomes– contain membrane-bound organelles that
compartmentalize the cytoplasm and perform specific functions
• Procaryotic cells: bacteria and archaea – no nucleus or other membrane-bound organelles
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Prokaryotic Profiles
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External Structures
• Appendages– Two major groups of appendages:• Motility – flagella and axial filaments
(periplasmic flagella)• Attachment or channels – fimbriae and pili
• Glycocalyx – surface coating
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Flagella
• 3 parts– filament – long, thin, helical structure
composed of proteins– hook- curved sheath– basal body – stack of rings firmly anchored
in cell wall
• Rotates 360o
• 1-2, or many distributed over entire cell
• Functions in motility
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Flagellar Function
Guide bacteria in a direction in response to external stimulus: chemical stimuli – chemotaxis; positive and
negativelight stimuli – phototaxis
Signal sets flagella into rotary motion clockwise or counterclockwise:counterclockwise – results in smooth linear
direction – runclockwise - tumbles
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Chemotaxis in bacteria
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Fimbrae
• Fine hairlike bristles from the cell surface
• Function in adhesion to other cells and surfaces
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Pili
• Rigid tubular structure made of pilin protein
• Found only in Gram negative cells
• Functions – joins bacterial cells for DNA transfer
(conjugation)– adhesion
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Conjugation
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Glycocalyx
• Coating of molecules external to the cell wall, made of sugars and/or proteins
• 2 types1. capsule - highly organized, tightly attached2. slime layer - loosely organized and attached
• Functions– Attachment and formation of biofilms– inhibits killing by white blood cells– protect cells from dehydration and nutrient loss
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Biofilms
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Biofilm on a Catheter
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The Cell Envelope
Composed of two basic layers:– cell wall and cell membrane
• Maintains cell integrity• Two generally different groups of
bacteria demonstrated by Gram stain:– Gram-positive bacteria: thick cell wall
composed primarily of peptidoglycan and cell membrane
– Gram-negative bacteria: outer cell membrane, thin peptidoglycan layer, and cell membrane
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Peptidoglycan
• Unique macromolecule composed of a repeating framework of long glycan chains cross-linked by short peptide fragments
• Provides strong, flexible support to keep bacteria from bursting or collapsing because of changes in osmotic pressure
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4 Bacterial Groups Based on Cell Wall Composition
1. Gram positive cells
2. Gram negative cells
3. Bacteria without cell walls
4. Bacteria with chemically unique cell walls
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Gram Positive Cell Wall
• Consists of – a thick, homogenous sheath of
peptidoglycan 20-80 nm thick– tightly bound acidic polysaccharides,
including teichoic acid and lipoteichoic acid
– cell membrane
• Retain crystal violet and stain purple
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Gram Negative Cell Wall
• Consists of– an outer membrane containing
lipopolysaccharide (LPS)– thin shell of peptidoglycan– periplasmic space– inner membrane
• Lose crystal violet and stain red from safranin counterstain
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Cell Membrane Structure
• Phospholipid bilayer with embedded proteins – fluid mosaic model
• Functions in:– providing site for energy reactions, nutrient
processing, and synthesis– transport into and out of the cell
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Cell Membrane Structure
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Cytoplasm
• Dense gelatinous solution of sugars, amino acids, & salts
• 70-80% water
• serves as solvent for materials used in all cell functions
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“Chromosome”
• Single, circular, double-stranded DNA molecule that contains all the genetic information required by a cell
• DNA is tightly coiled around protein, aggregated in a dense area called the nucleoid
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Plasmids
• Small circular, double-stranded DNA• Free or integrated into the chromosome• Duplicated and passed on to offspring• Not essential to bacterial growth &
metabolism• May encode antibiotic resistance, tolerance
to toxic metals, useful enzymes & toxins• Used in genetic engineering- readily
manipulated & transferred from cell to cell
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Ribosomes
• Made of 60% ribosomal RNA & 40% protein
• Consist of 2 subunits: large & small• Procaryotic differ from eucaryotic
ribosomes in size, and number of proteins
• Site of protein synthesis• All cells have ribosomes
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Ribosomes
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Endospores
• Resting, dormant cells• Produced by some G+ genera: Clostridium,
Bacillus & Sporosarcina• Have a 2-phase life cycle – vegetative cell &
an endospore
• Sporulation -formation of endospores
• Germination- return to vegetative growth • Hardiest of all life forms• Withstand extremes in heat, drying,
freezing, radiation & chemicals
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Sporulation Cycle
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Endospores
• Environmental resistance linked to high levels of calcium & dipicolinic acid
• Dehydrated, metabolically inactive• Thick coat• Longevity verges on immortality, 25-250
million years• Pressurized steam at 121oC for 20-30
minutes will destroy
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3 Basic Shapes of Bacteria
• Cocci - spherical
• Bacilli - rod
• Spiral - helical, comma, twisted rod, spirochete
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Methods in Bacterial Identification
1. Microscopic morphology2. Macroscopic morphology – colony
appearance3. Physiological / biochemical
characteristics4. Serological analysis5. Genetic & molecular analysis
• G + C base composition• DNA analysis using genetic probes• Nucleic acid sequencing & rRNA analysis
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Major Taxonomic Groups of Bacteria
• Domain Archaea – primitive, adapted to extreme habitats and modes of nutrition
• Domain Bacteria - – Phylum Proteobacteria – Gram-negative
cell walls– Phylum Firmicutes – mainly Gram-positive
with low G + C content – Phylum Actinobacteria – Gram-positive
with high G + C content
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• species –a collection of bacterial cells which share an overall similar pattern of traits in contrast to other bacteria whose pattern differs significantly
• strain or variety – a culture derived from a single parent that differs in structure or metabolism from other cultures of that species (biovars, morphovars)
• type – a subspecies that can show differences in antigenic makeup (serotype or serovar), susceptibility to bacterial viruses (phage type) and in pathogenicity (pathotype)
Bacterial Naming Conventions
Procaryotes with Unusual Characteristics
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Rickettsias• Very tiny, gram-negative bacteria• Most are pathogens that alternate between
mammals and fleas, lice or ticks• Obligate intracellular pathogens • Cannot survive or multiply outside of a host cell• Cannot carry out metabolism on their own • Rickettsia rickettisii – Rocky Mountain spotted
fever• Rickettsia prowazekii – epidemic typhus• Coxiella burnetti – Q fever
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Chlamydias• Tiny• Obligate intracellular parasites• Not transmitted by arthropods• Chlamydia trachomatis – severe eye
infection and one of the most common sexually transmitted diseases
• Chlamydia psittaci – ornithosis, parrot fever
• Chlamydia pneumoniae – lung infections
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Mycoplasmas
• Naturally lack a cell wall• Membranes stabilized by sterols,
resistant to lysis• Extremely small• Range in shape from filamentous to
coccus or doughnut shaped• Mycoplasma pneumoniae – causes
atypical pneumonia in humans
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Variations in the Shape of Mycoplasma pneumoniae
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Free-living Nonpathogenic Bacteria
• Photosynthetic bacteria– Cyanobacteria– Green & purple sulfur bacteria
• Gliding, fruiting bacteria
• Appendaged bacteria– produce an extended process of the cell
wall in form of a bud, stalk or long thread
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Archaea• Constitute third Domain• Appear more closely related to Domain Eucarya
than to bacteria• Contain unique genetic sequences in their rRNA• Have unique membrane lipids & cell wall
construction• Live in the most extreme habitats in nature,
extremophiles• Includes: methane producers,
hyperthermophiles, extreme halophiles, and sulfur reducers