Progress in PhotosynthesisResearch

Volume 2

Proceedings of the Vllth International Congress on Photosynthesis

Providence, Rhode Island, USA, August 10-15, 1986

edited by

J. BIGGINS

Division ofBiology and Medicine, Brown University

Providence, RI 02912, USA

UNiVERSITATSBIBLJOTHEKHANNOVER

TECHNISCHE

INFORMATIONSBIBLIOTHEK

1987 MARTINUS NIJHOFF PUBLISHERS ±4.a member of the KLUWER ACADEMIC PUBLISHERS GROUP

DORDRECHT / BOSTON / LANCASTER

VII

CONTENTS TO VOLUME II

General Contents V

Contents Volume II VII

Preface XXIII

Acknowledgments XXV

Local Organizing Committe XXVII

1. Components and Pigment-protein Complexes a) b) c) d)

Structural Principles of the Antenna System of

Photosynthetic Organisms 1

H. Zuber

The Molecular Size of Photosynthetic Membrane Protein Complexes 9

Jonathan H.A. Nugent

Structural Studies on the Light-harvesting Polypeptidesof RP. Acidophil 13

R.A. Brunisholz, I. Bissig, E. Niederer, F. Surer, H. Zuber

Localization and Comparative Chemical Activity of SH-groupsof the Subunits in the Reaction Centers from

Rhodopseudomonas Sphaeroides R-26 17

A.A. Solov'ev, Yu.E. Erokhin

Topological Studies on the Light-harvesting-pigment-protein-complexes in the Membrane of Rhodospirillum Rubrum 21

Reinhard Bachofen, Robin Ghosh, Rosmarie Schwerzmann

Preparation of Subunit Forms of the Light-harvesting Complex of

Rhodospirillum Rubrum 25

P. Parkes-Loach, J. Riccobono, P. Loach

Chemical Modification of Tryptophans and Lysines in the B880

Light-harvesting Protein of Rhodospirillum Rubrum 29

Germaine Gogel, Holly March, Andrew Friedrich, Paul Schwartz

Studies of Chloroplast Photosystem I Using Monoclonal Antibodies 33

R.E. Slovacek, M.A. Harvey

VIII

Bound Acceptors of Photosystem I 37

W. Lockau, H.-U. Schoeder, W. Nitschke, K. Ziegler

Photosystem I Complex of Higher Plants: Purification and

Characterization of its Two Chlorophyll-protein Complexes 41

Rachel Nechushtai, Camille C. Peterson, J. Philip Thornber

Preparation and Properties of Photosystem-1 Reaction

Center Complex 45

Tetsuo Hiyama, Akira Katoh, Tokuro Shimizu, Keisuke Inoue,

Akio Kubo

The Organization of the Fe-S Acceptors of Photosystem 1 49

Birger Lindberg M0ller, Barbara Ann Halkier, Peter Bordier H0j

Analysis of Isolated PS I Polypeptides for Acid Labile Sulfide 53

Peter Bordier H0j, Barbara Ann Halkier, Birger Lindberg M0ller

Effects of Mercurials on Iron-sulfur Centers of Photosystem I of

Anacystis Nidulans 57

Yasuo Kojima, Tetsuo Hiyama, Hidehiro Sakurai

Light-harvesting Chlorophyll-proteins of Barley Photosystem I 61

R. Bassi, D. Simpson

Measurement of the Optical Cross Section of Photosystem I

in Chlorella 65

Nancy L. Greenbaum, David Mauzerall

Isolation of a Photosystem II Reaction Center Consisting of y and b

Subunits (D-l and D-2) and Cytochrome b-559 69

Kimiyuki Satoh, Osamu Nanba

Quantitation of Plastoquinone and Functional Electron Carriers in the

Photosystem II Reaction Center Complex 73

Yuichiro Takahashi, Kimiyuki Satoh

Identification of QA_, QB_ and Manganese-carrying Subunit

Polypeptides of Synechococcus Photosystem II Reaction

Center Complex 77

Kazuhiko Satoh, Sakae Katoh

The Organisation of Photosystem II Chlorophyll-proteins 81

R. Bassi, D. Simpson

Phosphorylated Polypeptides of the Photosystem 2 Core Complex 89

J.B. Marder, P.A. Millner, K. Gounaris, J. Barber

Stoichiometry and Turnover Rates of Photosystem 2 Core

Polypeptides of the Halotolerant Green Alga Dunaliella Salina 93

Kleoniki Gounaris, James Barber, Uri Pick

Variable and Conserved Characteristics of Photosystem 2 of Spinachand of the Halotolerant Green Alga Dunalliela Salina 97

James Barber, Uri Pick, Kleoniki Gounaris

IX

Organization of Photosystem II Core and Light-harvesting Complexes 101

Gary F. Peter, J. Philip Thornber

Chemical Composition of Photosystem II Reaction Centers (PSII):Phosphorylation of PSII Polypeptides 105C. De Vitry, B.A. Diner, Y. Lemoine

Topology of Polypeptides and Redox Components in PS II and the

Cytochrome b/f-complex 109

Achim Trebst

Does the Rapidly Metabolized 32 kDa Protein Carry the

Reaction Center of PS II? 113Otto Machold

Analysis of Photosystem II Proteins by Two-dimensional

Electrophoresis 117Leslie J. Baier, Neil R. Bowlby, Wayne D. Frasch

The 5 kDa, 10 kDa and 22 kDa Polypeptides of Higher Plant

Photosystem II 121

Ulf Ljungberg

Low Molecular Weight Polypeptides in Photosystem II and Protein

Dependent Acceptor Requirement for Photosystem II 125

Tomas Henrysson, Ulf Ljungberg, Lars-Gunnar Franzen,Bertil Andersson, Hans-Erik Akerlund

Characterization of a Monoclonal Antibody which Reacts with the

49 kDa Polypeptide of Photosystem II from Spinach 129

Terry M. Bricker, Laurie K. Frankel

Oxidation Reduction Properties of the Iron (Q4(>0) in PSII in the

Cyanobacterium Synechocystis 6714 133

Stenbjorn Styring, Claudie Vernotte, Anne-Lise Etienne

Pigment Composition of Pigment-protein Complexes from the

Dinoflagellate Gonyaulax Polyedra 137

J. Knoetzel, I. Damm, L. Rensing

Monoclonal Antibodies to the Light Harvesting Chlorophyll-proteinComplex 141

S.C. Darr, C.J. Arntzen, S.C. Somerville

Isolation and Immunological Characterization of a Carotenoid-

binding Protein from the Cyanobacteria Synechocystis sp. PCC6714

and Anacystis Nidulans R2 145

George S. Bullerjahn, Harold C. Riethman, Louis A. Sherman

Isolation and Characterization of a Carotenoid-associated Membrane

Protein from the Cyanobacterium, Anacystis Nidulans R2 149

Kazumori Masamoto, Harold C. Riethman, Louis A. Sherman

X

Structural Principles of Cryptomonad Biliproteins and CyanobacterialPhycobiliproteins 153

Walter A. Sidler, Hansueli Nutt, Barbara Kumpf, Herbert Zuber,Werner Wehrmeyer, Anfried Brenzel

Non-hemidiscoidal Phycobilisome in Cyanophytes 157

Kaori Ohki, Yoshihiko Fujita

Comparative Immunology of the Phycobilisome Linker Polypeptides 161

Barbara A. Zilinskas, Dawn A. Howell

Structural Studies on Cytochrome b6 and the Rieske FeS-centcr 165

W. Nitschke, G. Hauska

Large-scale Isolation of Cytochrome bjf Complex from

Spinach Chloroplasts 173

Michael T. Black, William R. Widgcr, William A. Cramer

Isolation and Characterization of the Q£>c-complex from the

Thermophilic Cyanobacterium Synechococcus 6716 177

F. Koppenaal, K. Krab, R. Kraayenhof

Photosynthetic Pigment Organization in Membranes of

Eucaryotic Plants 181

Jeanette Brown

Organization of the Photosynthetic Apparatus in Macrocystis Pyrifera 185

Barbara Smith, Anastasios Melis

Pigment-protein Complexes and Light Harvesting in

Eustigmatophyte Algae 189

J. Chrystal, A.W.D. Larkum

Immunological Studies on the Chlorophyll a + b Antenna Complexesof Photosystem I and Photosystem II 193

Michael J. White, Beverley R. Green

Large-scale Preparation of Highly Purified Chloroplast Lipids for

Liposome Permeability Studies 197

M.S. Webb, B.R. Green

The Association between Galactolipids and Chlorophyll-proteinComplexes 201

W. Patrick Williams, Peter J. Dominy

Galactosyltransferase Activities in Intact Spinach Chloroplasts and

Envelope Membrane

Involvement of Galactolipid: Galactolipid Galactosyltransferase 205

Johan W.M. Heemskerk, Frans H.H. Jacobs, Vincent A. Tromp,J.F.G.M. Wintermans

Characterization of Galactosyltransferases in Spinach ChloroplastEnvelope Membranes

UDPGal-dependent and -independent Galactolipid Synthesis 209

Jef. F.G.M. Wintermans, Martin A.M. Scheijen, Frans H.H. Jacobs,

Johan W.M. Heemskerk

XI

Effects of Monogalactosyldiacylglycerol (MGDG) Depletion on

Photosynthetic Activities in Oat Thylakoid Membranes 213

Paul-Andre Siegenthaler, Christian Giroud

Galactolipids and Thylakoid Intramembrane Particles 217

Kenneth R. Miller, Jules S. Jacob

Reconstitution of Functional Phycobilisome-thylakoid Association in

Cyanobacteria 221

J.C. Thomas, D. Hoursiangou-Neubrun, A. Mousseau

Molecular Organization of the Inner Etioplast Membranes 225

Eva Selstam, Anna Widell

Surface Properties of the Prolamellar Body Membrane 229

Anna Widell, Eva Selstam, Kjell Edman

2. Stoichiometry of the Photosystem I and Photosystem II

P700 Spectra and Concentrations in Several Plants and a

Cyanobacterium 233

W.-J. Lee, H.B. Pakrasi, J. Whitmarsh

Recovery Time of Physiologically Inactive Photosystem II

Reaction Centers Measured by the Elecrochromic Absorbance

Change at 515nm 237

R. Chylla, G. Garab, J. Whitmarsh

Identification of Photosystem II Centers Inactive in PlastoquinoneReduction 241

Thomas Graan, Donald R. Ort

Photosystem Stoichiometry in Higher Plant Chloroplasts 245

Steven W. McCauley, Anastasios Melis

Cause and Effect Relationship between Environmental Conditions

and Photosystem Stoichiometry in Synechococcus 6301 249

Annamaria Manodori, Anastasios Melis

Differential Phosphorylation of Thylakoid Membrane Polypeptides

During Chloroplast Adaptation to Light Quality 253

Richard E. Glick, Ulla K. Larsson, Anastasios Melis

The Physiological Significance of Thylakoid Membrane Protein

Phosphorylation 257

Anastasios Melis, Xingwang Deng

Development of the Light-harvesting Antenna of the Photosystems in

Chlorophyll-6 Deficient Mutants 261

Maria L. Ghirardi, Anastasios Melis

Comparative Studies on the Light Absorption Capacity of

Photosystem II and Photosystem I 265

Michael Spangfort, Anastasios Melis, Berth Andersson

XII

3. Lateral Heterogeneity and Stacking

An Analysis of the Salt-induced Rise of Chlorphyll Fluorescence to

Investigate Ion-specific Effects between and within Valency Groups 269

William R. Newell, Stephen J. Zara, James Barber

Differential Thylakoid Membrane Stacking in Mangrove and Spinach 273

Christopher Preston, Nicholas D. Mackie, Christa Critchley

The Role of LHCII in Thylakoid Membranes 277

R. Bassi, D. Simpson, R. Barbato, G. H0yer-Hansen, U. Hinz,

G.M. Giacometti

Subfractionation of Inside-out Thylakoid Vesicles-preparation of Pure

Photosystem II Particles without Using Detergent 281

Per Svensson, Per-Ake Albcrtsson

Composition of the Marginal Regions of Thylakoid Membranes 285

Andrew N. Webber, Kathryn A. Platt-Aloia, William W. Thomson,

Robert L. Heath

Effect of Divalent Cations on the Aging of Wheat Chloroplasts 289

Jai Parkash, Dinesh K. Sukumaran, G.S. Singhal

4. Localization of Membrane Components

Cytochrome f Exists as Two Functionally Separate Pools in

Pea Thylakoid Membranes 293

David R. Alfred, L. Andrew Staehelin

Structural Identification of the Oxygen-evolving Apparatus on the

Lumenal Surface of Freeze-etched Thylakoid Membranes 297

L.A. Staehelin, M. DeWit, M. Seibert

Lateral Distribution of the Photosystem I Complex between the

Appressed and Non-appressed Regions of Spinach ThylakoidMembranes: An Immunocytochemical Study 301

Jan M. Anderson, David J. Goodchild

Differential Solubilization of the Integral Electron Transport

Complexes from the Thylakoid Membrane of Spinach Chloroplasts.Localization of Photosystem I, Photosystem II and the Cytochrome

bk-f Complex 305

Peter J. Morrissey, Steven W. McCauley, Anastasios Melis

Immunogold Localization of the Phycobiliprotein of a CryptophyteAlga to the Intrathylakoidal Space 309

Laurel Spear-Bernstein, Kenneth R. Miller

Immunogold Labeling of Thylakoid Membranes 313

Jenny E. Hinshaw, Kenneth R. Miller

XIII

Assignments of Stroma-exposed PSII Proteins after Two-dimensional

Electrophoresis Using Affinity Purified Polyclonal Antibodies 317

Paul D. Hierholzer, Stan Ivey, Steven P. Berg

Determination of the Insideout to Rightsideout Ratio of:

1) Thylakoids Exposed to Phase Partition, 2) Thylakoids Washed with

EDTA, and 3) Detergent Derived PSII Membranes 321

Mark Zitkus, Paul D. Hierholzer, Stan Ivey, Steven P. Berg

Localization of the B/F Complex by Freeze-fracture Analysis of the

Chlamydomonas Reinhardtii and Chlorella Sorokiniana Mutants

Lacking in this Complex 325

J. Olive, F.A. Wollman

Immunocytochemical Studies on the Organization of ThylakoidMembrane Proteins 329

O. Vallon, F.A. Wollman, J. Olive

Collapse Mechanism of Some Carotenoid Monomolecular

Films-membrane Model 333

Maria Tomoaia-Cotisel, J. Zsako, E. Chifu, D.A. Cadenhcad,

H.E. Ries Jr.

5. Effects of Environmental Changes and Growth Conditions

Effect of Daylength on Thylakoid Composition and Ultrastructure in

Brassica Rapa ssp. Oleifera 339

Arja Nurmi

Adaptation of Barley Thylakoid Membranes to Changing LightIntensity 343

W.R. de la Torre, K.O. Burkey

Pigment-protein Complexes of Algal Thylakoid Membranes:Variations in Pattern, Pigment Composition and Reaction Centre II

Types During the Cell Cycle of Chlorella Fusca and after Adaptationto Low Light Intensities 347

L.H. Grimme, I. Damm, D. Steinmetz, D. Scheffczyk

On the Protective Role of Carotenoids in the PS I Reaction Centre

and LHC I Complexes of the Thylakoid Membrane 351

I. Damm, J. Knoetzel, L.H. Grimme

Response of Chlamydomonas Reinhardtii Reaction Center

Stoichiometry to Light Intensity Variation: A Spectroscopic and

LDS-page Analysis 355

Patrick J. Neale, Anastasios Melis

Effects of Nitrate Nutrition on Chloroplast Composition and

Photosynthetic Activities in Wheat 359

F. Macnab, D.W. Lawlor, N.R. Baker, A.T. Young

XIV

The Effect of Different Growth Light Intensities on Photo-system II

Components 363

A. Wild, M. Hopfner, W. Riihle, M. Richter

Changes in Pool Sizes of Photosynthetic Electron TransportComponents and Rubisco with Growth Irradiance - Evidence for the

Limiting Step in Light Saturated Photosynthesis 367

Assaf Sukenik, John Bennett, Paul G. Falkowski

Chlorophyll-protein Complexes Changes Associated with ChloroplastDevelopment in a Virescent Pewtunia Hybrida Mutant 371

Y. Lemoine, G. Zabulon, A. Cornu

Altered Photosynthetic Pigment Complements in Mutants of the

Cyanobacterium Synechococcus Sp. spp 7002 375

Robt. Webb, Kathleen Howlcy, John Brady, Thomas Punnett

Characterization of a Novel Chlorophyll-binding Component of

Cyanobacterial Thylakoids 379

Harold C. Riethman, Louis A. Sherman

Some Aspects of Altered Structure and Functioning of the

Photosynthetic Apparatus in Phytochrome-lcss Mutants of Tomato 383

Willcm F. Buurmeijer, Theo A. Roelofs, Willem J. Vredenberg

6. Quinone Acceptors

Some Functional Characteristics of Reaction Centers from

Rhodopseudomonas Viridis 387

C.A. Wraight, R.J. Shopes, J.C. McComb, L.M.A. Levine, D.

Holten

Herbicide-resistant Reaction Center Mutants of

Rhodopseudomonas Viridis 397

R.J. Shopes, C.A. Wraight

Light Induced Proton Binding-unbinding Dynamics in Reaction

Centers from Rhodobacter Sphaeroides 401

P. Maroti, C.A. Wraight

An Inhibitor-resistant Mutant Impaired in Ubiquinol Oxidation

in the Ubiquinol Cytochrome C, Oxidoreductase of

Rhodobacter Capsulatus 405

D.E. Robertson, F. Daldal, D.C. Wang, P.L. Dutton

Stigmatellin and Other Electron Transfer Inhibitors as Probes for the

QB Binding Site in the Reaction Center of Photosynthetic Bacteria 409

Kathleen M. Giangiacomo, Dan E. Robertson, M.R. Gunner,

P. Leslie Dutton

Q Pool Interactions in the Reaction Center-cytochrome be. ComplexHybrid Proteoliposome System 413

C.C. Moser, M.R. Gunner, P.L. Dutton

XV

Rcconstitution of Subunits LM of the Reaction Center from

Rhodopseudomonas Sphaeroides into Liposomes 417

H. Celis, M. Ludgar

Covalent Binding of Halogen Substituted 1,4-quinones to Proteins of

the Thylakoid Membrane 421

Ralf Dostatni, Klaus Masson, Walter Oettmeier

Competition of Inhibitors with the Secondary Quinone in Dark

Adapted Thylakoid Membranes 425

Shinich Taoka, Anthony R. Crofts

Kinetics of the Changes in Oxidation-reduction States of the

Acceptors and Donors of Photosystem II in Pea Thylakiods Measured

by Flash Fluorescence 429

Howard Robinson, Antony Crofts

The Effect of pH and Flash Frequency on Electron Transfer throughthe Quinone Acceptor Complex of PS II in Bicarbonate Depleted or

Anion Inhibited Thylakoid Membranes 433

Julian J. Eaton-Rye, Govindjee

Manipulations of the Pool Characteristics of the Quinone Electron

Donor and Acceptor to Ubiquinol-cytochrome c Oxidoreductase.

Evidence for Redox Contact between the Cytochrome b Hemes 437

Simon DeVries, Christopher C. Moser, Dan E. Robertson,

P. Leslie Dutton

7. Cytochromes (b-f) Complex

Inhibition of Electron Transfer in the Cytochrome b/fComplex by

NQNO and DBMIB: Evidence for Two Quinone Binding Sites 441

R.W. Jones, J. Whitmarsh

The Electrogenic Reaction and Proton Release During Quinol

Oxidation by the Cytochrome blf Complex 445

R.W. Jones, J. Whitmarsh

Electron and Proton Transfer in the Cytochrome bf Complex 453

P.R. Rich, P. Heathcote, D.A. Moss

The effect of Pre-reduction of the fe-type Cytochromes on Electron

Transport through the Cytochrome bf Complex in Chloroplasts. 461

David A. Moss, Peter R. Rich

Studies of the Chloroplast Cytochrome b(-f Complex Incorporatedinto Liposomes 465

I. Willms, R. Malkin, R.K. Chain

The Plastoquinone/Plastoquinol Oxidoreductase Sites of the

Chloroplast Cytochrome blf Complex 469

Daniel P. O'Keefe

XVI

Proposed Alignment of Amino Acid Sequences for Cytochromes cl

and f and Identification of Putative Binding Sites for Cytochrome c

and Plastocyanin 473

Dan J. Davis

Quinol Specificity of the Cytochrome b6f-complex - Evidence againsta Permanently Bound Plastoquinone Molecule 477

W. Nitschke, I. Berthold, G. Hauska

On the Trans-membrane Electron Transport Pathway of

Cytochrome b6 481

William A. Cramer, Mark E. Girvin, William R. Widger,Dierk Mende, Michael T. Black

The Antimycin-binding Site of Thylakoid Membranes from

Chloroplasts 485

Elizabeth C. Davies, Derek S. Bendall

Inhibition of High Energy State Quenching in Spinach Chloroplastsby Low Concentrations of Antimycin A 489

Kevin Oxborough, Peter Horton

Electron Transfer from Quinol to Cytochrome B-561 is Not Diffusion

Limited in the Ubiquinol: Cyt C, Oxid Oreductase Complex of

Rps. Sphaeroides 493

Zhenggan Wang, Edward A. Berry, Antony R. Crofts

Characterization by EPR Spectroscopy of Cytochrome b-562 Isolated

from the Cytochrome b-c. Complex of Rhodopseudomonas

Sphaeroides R-26 497

Koh Iba, Ken-ichiro Takamiya, Mitsuo Nishimura, Shigeru Itoh

8. Lateral Electron Transport, Connectivity between Photosystems

Plastoquinol and Plastoquinone Diffusion in Model Membranes 501

Mary F. Blackwell, Kleoniki Gounaris, James Barber

Temperature Dependence of DF-kinetics and PQ-conductivity 505

H. Krause, V. Gerhardt, J. Wachtveitl

A Carbon-13 NMR Study of Plastoquinone in Membranes 509

Adele Post, Roger G. Hiller, Bruce A. Cornell

Lateral Electron Transport in Thylakoids of Higher Plants 513

Wolfgang Haehnel, Rowan Mitchell, Andreas Spillmann

9. Plastocyanin

On the Electron Transfer from Plastocyanin to P700 521

Wolfgang Haehnel, Rafael Ratajczak, Rowan Mitchell

XVII

Use of Affinity Chromatography to Purify Plastocyanin and

Cytochrome f 525

George Anderson, Steven A. Molnar, Elizabeth Gross

The Effect of Ethylenediamine Chemical Modification on the

Electron Transport Properties and Redox Potential of SpinachPlastocyanin 529

George Anderson, Elizabeth Gross

The Effect of pH on the Near UV Circular Dichroic Spectra of

Parsley Plastocyanin 533

Stewart Durell, Elizabeth Gross, James Draheim

10. PSI-cyclic Electron Transport

Ferredoxin-dependent Cyclic Electron Transport and

Photophosphorylation 537

Hans J. Rurainski, Sieglinde Borchert, Ralf Heupel

Cyclic Electron Transport Around Photosystem I in

Washed Thylakoids 541

Gyozo Garab, Geoffrey Hind

Ferredoxin-NADP+ Oxidoreductase: Studies on the ThylakoidMembrane Bound Enzyme 545

Hans C.P. Matthijs, Sean J. Coughlan, Geoffrey Hind

Inhibitors of Ferredoxin-catalysed Cyclic Photphosphorylation in

Chloroplasts 549

Pravin K. Patel, Derek S. Bendall, Stuart M. Ridley

11. Inhibition and Environmental Effects

Photosynthetic Control of Electron Transport in Leaves of Phaseolus

Vulgaris: Evidence for Regulation of Photosystem 2 by the

Proton Gradient 553

Engelbert Weis, J. Timothy Ball, Joseph Berry

Modification of the Redox State of PSII Electron Acceptors In Vivo

by Low Levels of Intercellular CO, 557

C.R. Ireland, N.R. Baker, S.P. Long

Analysis of the a and /3 Centres of Photosystem II, in Presence of

Atrazine and TMPD 561

Ranjana Paliwal, Gauri S. Singhal

Senescence of Chloroplasts in Fern Leaves 565

Basanti Biswal

XVIII

The Role of Cu in the Regulation of the PhotosyntheticElectron Flow 569

Gabor Horvath, Magdolna Droppa, Adam Wolak, Laszlo I. Horvath,Tibor Farkas

Copper in Photosystem II 573

Peter R. Sibbald, Beverley R. Green

Inhibition of the Photosynthetic Electron Transport of Isolated

Chloroplasts by Hemolyzed Rabbit Sera 577

Brad L. Upham, Kriton K. Hatzios, Leland S. Jahnke

Effect of Dimethylaminoethyl /;-chiorophenoxyacetate (Centro-

phenoxine) on Chloroplast Electron Transport Activity 581

S.C. Sabat, N. Mohanty, S. Hoshina, P. Mohanty

Effect of Resorcinols on Electron Transport in Pea Chloroplasts 585

Jan Kieleczawa, Agata Szalewicz, Arkadiusz Kozubek, Elzbicta KuligAscorbate as a Pro-oxidant and an Anti-oxidant During a

Mehler Reaction 589

Leland S. Jahnke

Effects of Deuterium Oxide on Photosynthetic Light Reactions 593

D.J. Kyle, P.W. Behrcns, R.M. Gladue, A.H. Syllaba, R.J. Radmer

Senescence and Heat Stress Induced Alterations of PhotosyntheticElectron Transport 597

Prasanna Mohanty

Oscillation in Photosystem II Activity of Chloroplasts Isolated from

Dark Adapted Wheat Seedlings 605

Shabbir A. Sayeed, Prasanna Mohanty

Diurnal Changes in Electron Transport Capacity in Pea Tylakoids 609

Warren Zipfel, Ruth Alscher, John Bower

12. General Aspects

Modelling of Photosynthetic Energy Conversion

I Description and Basic Principles of the Model 613

Willem J. Vredenberg, Olaf van Kooten, Jan F.H. Snel

Modelling of Photosynthetic Energy Conversion

II. Charge Separation and Secondary Electron Flow 617

Jan F.H. Snel, Olaf van Kooten, Willem J. Vredenberg

Modelling of Photosynthetic Energy Conversion

HI. Electrochemical Events at the Membrane 621

Olaf van Kooten, Jan F.H. Snel, Willem J. VredenbergPhotoacoustic Action Spectra of Bundle-sheath Cells of Zea Mays 625

R. Popovic, R.M. Leblanc, M. Beauregard

XIX

Photoacoustic Study of Photosynthetic Activity in Bundle-sheath Cells

of Zea Mays 629

M. Beauregard, R.M. Leblanc, R. Popovic

Sulfide-dcpendent Electron Transport in Thylakoids from the

Cyanobacterium Oscillatoria Limnetica 633

C. Sybesma, L. Slooten

Energetics of Hydrogen Recycling in Nitrogen-fixing Cyanobacteria 637

Helmar Almon, Peter Boger

Photosynthesis, Nitrogen Fixation, Ammonia Photoproduction and

Structure of Anabaena Azollae Immobilized in Natural and

Artificial Systems 641

D.J. Shi, D.O. Hall, P.S. Tang

Sustained Ammonia Production by Immobilized Cyanobacteria 645

M. Brouers, H. de Jong, D.J. Shi, K.K. Rao, D.O. Hall

Cellular Biochemical Activities and Induction of Synchronous Growth

in a Unicellular Aerobic Nitrogen-fixing Cyanobacterium,Synechococcus Sp. Strain Miami BG 043511 649

T. Arai, A. Mitsui

Inhibition of Respiration by Short Saturating Flashes in

Chlamydomonas: Evidence for a Chlororespiration 653

Gilles Peltier, Jacques Ravenel, Andre Vermeglio

Studies on the Linking of Photosynthetic and Respiratory Electron

Transport Chains and Phosphorylation 657

Li Shu-jun, Xiao Jian-ping, Chong Qiu-chen, Cai Jian-ping,Xu Ya-nan

Cytochrome Oxidase of Photosynthetically Grown

Rhodopseudomonas Sphaeroides 661

Edward A. Berry, Antony R. Crofts

A Portable Double-flash Spectrophotometer for Measuring the

Kinetics of Electron Transport Components in Intact Leaves 665

David Kramer, Omar Adawi, Philip D. Morse, II, Antony R. Crofts

13. Higher Plants

Dynamic Aspects on the Organization of the Thylakoid Membrane 669

Berth Andersson, Cecilia Sundby, Ulla K. Larsson, Pirkko Maenpaa,Anastasios Melis

Different Polypeptide Composition of the Peripheral and TightlyBound Subpopulations of LHCII 677

Ulla K. Larsson, Cecilia Sundby, Bertil Andersson

XX

Interplay between Environmental and Metabolic Factors in the

Regulation of Electron Transport in Higher Plants 681

Peter Horton

The Importance of Membrane Surface Electrical Charge on the

Regulation of Photosynthetic Electron Transport by Reversible

Protein Phosphorylation 689

Alison Telfer

A Light-sensitive Photoregulatory Mutant in Maize Deficient in LHCI

and the 'Mobile' Chlorophyll a/b LHCII 697

B. Greene, D.R. Allred, D. Morishige, L.A. Staehelin

Immunolocalization of the CHL a/b-light Harvesting Complex and

CP29 under Conditions Favoring Phosphorylation and

Dephosphorylation of Thylakoid Membranes (State 1-Statc 2

Transitions) 701

Terri G. Dunahay, L. Andrew Staehelin

Comparison between the Effects of Cations, State I-State II

Transitions and Protein-phosphorylalion on LifeTime Components of

Chlorophyll Fluorescence 705

J-M. Briantais, M. Hodges, I. Moya

Does a Correlation between the Xanthophyll Cycle and

State 1-State 2 Changes Exist? 709

Erhard Meek, Rcto J. Strasser

Altered Lipid Matrix Changes Energy Distribution in Thylakoids 713

Josef A. Graf, Relo J. Strasser, Ulrich Kull

Energy Pipeline Model of the Photosynthetic Apparatus 717

Reto J. Strasser

Influence of Thylakoid Protein Phosphorylation on PS I DependentPhotophosphorylation and on Electron Transport 721

Giorgio Forti, Paola M.G. Grubas

The Kinetics of Phosphorylation of the Light Harvesting Chlorophylla/b Protein and Associated Changes in Photosystem Cross Section and

Energy Spillover 725

G. Zucchelli, K. Islam, R.C. Jennings

Reversal Movement of Sub-population of Light HarvestingChlorophyll Protein Complexes LHC-II and LHC-I between Grana

and Stroma Lamellae under Different Conditions 729

T.Y. Kuang, J.G. Yuan, C.Q. Tang, Q.D. Zhang, S.Q. Lin

A Possible Redistribution of Excitation Energy in SpinachChloroplasts Due to Nitrite Ions 733

John Sinclair

Effect of Light State Transitions on the Apparent Absorption Cross

Section of Photosystem II in Chlorella 737

Paul G. Falkowski, Yoshihiko Fujita

XXI

Multiple Effects of Trypsin- and Heat-treatments on the

Ultrastructure and Surface Charge Density of Pea ChloroplastMembranes. Influence on P700+ Parameters 741

Alexander G. Ivanov, M. Velitchkova, D. Kafalieva

Steady-state Kinetics of Chlorophyll-fluorescence and LightScattering 745

Jorg Kolbowski

Extreme Changes in the Distribution of Excitation Energy in the

Photochemical Apparatus of Intact Leaves Induced by ProgressiveHeat Stress 749

Michel Havaux, Ora Canaani, Shmuel Malkin

Environmental Control of Photosynthetic Enhancement in Palisade

Mesophyll Chloroplasts Measured by Photoacoustic Spectroscopy 753

Thomas Punnet

14. Organisms Containing Phycobilins

Regulation of Photosynthetic Unit Function byProtein Phosphorylation 757

John F. Allen, Michael A. Harrison, Nigel G. Holmes,Conrad W. Mullineaux, Christine E. Sanders

The 18.5 kDa Phosphoprotein of the Cyanobacterium Synechococcus6301: A Component of the Phycobilisome 761

Christine E. Sanders, John F. Allen

State 1-State 2 Transitions in Synechococcus 6301 Are Influenced byRespiratory Electron Flow; Evidence for the Control of

Excitation Energy Distribution by the Redox Level of Plastoquinonein Cyanobacteria. 765

Conrad W. Mullineaux, John F. Allen

Control of State 1-State 2 Transitions in the Blue-green AlgaNostoc Muscorum 769

Ora Canaani

The Relationships Between Protein Kinase Activity and Chlorophyll a

Fluoresence Changes in Thylakoids from the CyanobacteriumSynechococcus 6301 773

John Biggins, Doug Bruce

Excitation Energy Transfer in Cryptomonas Ovata. Preillumination

Dependent Changes in 77K Picosecond Time Resolved Fluorescence

Emission Spectra 777

Doug Bruce, John Biggins, Sylvain Charbonneau, Mike Thewalt

Excitation Energy Redistribution in Cryptomonas Ovata 781

Ursula K. Snyder, John Biggins

XXII

Functional Interactions of Anions and Cations with the Outer Surface

of Intact Thylakoids: Studies with the Blue-green Bacterium AnacystisNidulans 785

George C. Papageorgiou, Georgia Sotiropoulou

Effect of Growth Temperature on Membrane Properties in a

Thermophilic Cyanobacterium (Synechococcus Sp.) 789

Mette Miller, Jens Z. Pedersen, Raymond P. Cox

Increased Energy Transfer from the Phycobilisomes to Photosystem II

in High Light Adapted Anabaena Cylindrica 793

Ilona Laczko, Elizabeth Kaiseva, Laszlo Zimanyi

15. Membrane Protein Kinases

Purification and Characterization of a Thylakoid Protein Kinase 797

Sean J. Coughlan, Geoffrey Hind

Reconstitution of LHC Phosphorylation by a Protein Kinase Isolated

from Spinach Thylakoids 801

Geoffrey Hind, Sean Coughlan

Identification of Phosphorylated Reaction Center Polypeptides in

Thylakoids of Chlamydomonas Reinhardtii and Pisum Sativum 805

M. Ikeuchi, F.G. Plumley, Y. Inouc, G.W. Schmidt

The Sensitivity of Pisum Sativum Thylakoid Membrane Protein

Kinase Activity to Inhibitors, Detergents and Heating 809

Julian P. Whiteleggc, Paul A. Millner, Kleoniki Gounaris,James Barber

Index of names 813