production of erythropoietin in pichia pastoris eporis – team 15 structurereviewprocess...
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Production of Erythropoietin in Pichia
PastorisEporis – Team 15
StructureReview Process ModelCapacity Business Model Future Steps
Team Eporis
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Abby LeistraStephen TubergenZion Lee Nick Giles
StructureReview Process ModelCapacity Business Model Future Steps
Background: What is Erythropoietin?
•Erythropoietin (EPO) is a glycoprotein hormone•Naturally made in kidneys•Promotes red blood cell production•Treats anemia caused by• Chronic kidney disease
• Cancer chemotherapy
• HIV therapy
• $4 Billion US Market in 2013
glycam.org
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Problem
• Chinese Hamster Ovary (CHO) Cells• Slow-growing• Many purification steps
nikon2.magnet.fsu.edu
Solution
2013.igem.org
• Pichia Pastoris Cells• Fast-growing• Easy purification of protein
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EPO Structure
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Nett, J. H.; Gomathinayagam, S.; et. al. J. Biotechnol. 2011, 157, 198-206.
•Polyethylene Glycol (PEG) • Large
• Negatively charged
Capacity Estimate
• How much? How big?
• Depends on:
• Market Volume
• Yeast Productivity
• Separation Recovery
en.wikipedia.org
• Bottom Line: 169 g EPO/yr → 8500 L/yr
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Process Model -PFD
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http://www.esslab.com/Hamilton/sensors/sensor_applications/bio.html
Process Model -Bioreactor
• Key Considerations• Oxygen Distribution• Impeller Shear Force
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http://2010.igem.org/File:UCL-BIOREACTSRS.png.
Process Model - Filtration
•Ultrafiltration
•Microfiltration
Houp, R. C. J. Validation Tech. 2009, 40-45.
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Business Model
•Three different alternatives• Start –up• Contracting • Leasing
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stcaustralia.org
Future Steps
•Provide detail to the process model• Calculating flow rates in and out of major process units• Determining process subunits
•Evaluate alternatives• Business model• Process units
•Optimize selected process model
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Questions?