PRESENTED BY:

MD. MOBAROK KAR IM

REG . NO. : 2 013431024

DEPT. OF GENETIC ENGINEERING AND B IOTECHNOLOGY,

SHAHJALAL UN IVERS ITY OF SC IENCE AND TECHNOLOGY .

Production of Biodiesel from

Botryococcus braunii

Biodiesel

Biodiesel is an alternative fuel similar to conventional or

‘fossil’ diesel.

Produced from

straight vegetable oil

animal fats

waste cooking oil.

Advantages of biodiesel

Renewable energy.

Environment friendly.

Have huge source for production.

Easy to produce.

Botryococcus Braunii

Colonial green microalgae.

Pyramid-shaped planktonic micro algae.

Found in temperate or tropical oligotrophic lakes and

estuaries.

Why Botryococcus braunii?

Easy to culture.

Have high amount oil content and that is up to 70% of

their dry weight.

Can grow dominantly.

Form lipid biofilm.

Why Botryococcus braunii?

Most useful lipids are extracellular lipids.

Can increase growth rate by rising CO2concentration.

Low cost in lipid production.

Culture of Botryococcus braunii

Very easy to culture.

Can culture in glass tube, in ponds, in haor,baor and any other fresh water source.

Waste water also preferable for culture.

Hydrocarbon content in Botryococcus braunii

Botryococcus braunii produces long chain hydrocarbons.

C25H48 (pentacosa-1,16-diene)

C27H52 (heptacosa-1,18-diene)

C29H56 (nonacosa-1,20-diene)

C31H60 (hentriaconta-1,22-diene)

C29H54 (nonacosta-1,20,22-triene)

Etc.

Production of Biodiesel

Botryococcus oils can be used as feedstock for hydrocracking inan oil refinery to produce octane, kerosene, and diesel.

Transesterification can not directly use to make biodiesel fromBotryococcus oils. Because Botryococcus oils are oils of vegetableorigin, they are inedible and chemically very different,being triterpenes, and lack the free oxygen atom needed fortransesterification.

After oil refining process, Trans-esterification process is used toproduce biodiesel.

Oil refinary system

Biodiesel production plant model

Other advantages of Botryococcus brauniiculture

Botryococcus braunii absorb high amount of carbon-di-oxide to maintain its growth rate. That’s why highcarbon-di-oxide concentration can increase its growthrate.

Botryococcus braunii can use to treat waste waterbecause it uptake dissolve phosphorus from water.

Disadvantages of botryococcus braunii culture

It create bloom in the culture environment that reduce the dissolve oxygen concentration in the culture area.

It also produce some toxin in the culture water.

It dominantly grows by suppressing the growth of other organisms by producing toxins.