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Solis BioDyneProduct Catalogue 2017Ambient Temperature Stable PCRand qPCR Reagents

www.sbd.ee

Dear Partners,Thank you for trusting Solis BioDyne with your research.

The cooperation we have built with you over the years has become more personalized and synergetic.

This ever closer relationship helps us understand your needs better and provide the solutions that precisely meet your requirements. In this 2017 edition of our catalogue you will see our most popular items, but keep in mind we love to discuss about your projects and help you tackle your PCR challenges.

Best regards,

Kadri Artma Managing Director

About UsSolis BioDyne manufactures high quality molecular biology reagents since 1995. The company is based in Tartu, Estonia, an international academic city with a growing biotechnology sector.

Our young and professional team is dedicated to provide:

• high quality products• cost effective solutions• quick and professional service

Our product line includes DNA polymerases, master mixes for PCR/qPCR and other reagents – all stable at ambient temperature. Each product batch goes through very strict quality control system based on ISO 9001:2015 standard.

Greetings from the Solis BioDyne Team

PRODUCT CATALOGUE 2017 1

Stability TAG Technology 2

qPCR Mix Specifications & Mix Compatibility Table 4

Dye-based qPCR Mixes 6

Probe-based qPCR Mixes 9

Product selection Guide:

Standard PCR Enzymes, Master Mixes and Other Enzymes 11

Hot-start PCR Enzyme and Mixes 12

Standard PCR Enzyme and Mixes 16

Other Enzymes:

TERMIPol DNA Polymerase; M-MLV Reverse Transcriptase 18

Other Reagents:

dNTP Mix and Set, DNA Ladders, DNA Loading Dye Buffers etc. 20

Ordering 23

Distributors 25

Product List 26

Table of Contents

Riia 185a, 51014 Tartu, EstoniaTel: +372 7409 960Fax: +372 7402 079E-mail: [email protected]: solis.biodyneWeb: www.sbd.ee

Solis BioDyneVAT No: EE100587614Reg. No: 10242922

Bank details:Swedbank ASIBAN code: EE692200221005142234SWIFT/BIC.: HABAEE2XBank address: Liivalaia 8, 15040 Tallinn, Estonia

2

Taq DNA Polymerase Stability TAG

Ice-free reaction set-upOur temperature stable enzymes allow you to change the way you work with enzymes that are usually extremely sensitive to tem-perature changes.

• more space on your work station and in your freezer• convenient working conditions• less energy used for making ice or keeping bench-top coolers

cold

Polypeptide Stabilization Technology: Stability TAG

REACTION SET-UP ON ICEREACTION SET-UP AT ROOM TEMPERATURE

3D MODEL OF FIREPOL® DNA POLYMERASE INCLUDING STABILITY TAG

Barleygenomic DNA

HOT FIREPol® DNA Polymerase

Mousegenomic DNA

Barleygenomic DNA

Mousegenomic DNA

FIREPol® DNA Polymerase

-20°C1 2 2 weeks at +35°C 3 4 weeks at +35°C

1 2 3 1 2 3 1 2 3 1 2 3

“ Solis BioDyne products are excellent products and meet the

following criteria:

• Quality: they give good results and a sharp signal without any

background noise.

• Cost: They are inexpensive, the order process is fast and the fact

they are storable/shipped at room temperature reduces the cost of

transportation.

• Convenience: no need to order separately the PCR products as

everything is inside the Mix which reduces pipetting errors. It is not

necessary to work in ice which helps to save energy and avoid the

deterioration of product due to power cuts or forgetting the

product at room temperature. ”ABDUL AZIZ WANE Biology and Sanitary Engineer Institut Pasteur Dakar, SENEGAL

Our technologyWe have genetically modified our enzymes to give them improved long term stability and enhanced stability at ambient temperatures. The addition of the Stability TAG (EU Patent EP2501716 and US Patent No 9,321,999) ensures that our enzymes are fully active even after keeping them at room temperature for a month!

Worry-free storageAccidents can happen to anyone. Our reagents will still remain fully active after a power outage has caused the loss of everything in your freezer, after someone has forgotten the reagents on the table overnight or if customs delays the delivery.

Our thermostable DNA polymerases HOT FIREPol® and FIREPol® show no activity loss after incubation at +35oC for up to 4 weeks.


Ca savings

Ca savings

Ca savings

No need to pay for shipping the ice

DRY ICEIN REGULAR

INSULATED BOX

ACTUALPRODUCT SIZE

Ice-free shippingOur Stability TAG technology means we can ship your order without dry ice or using large insulation boxes. Which is better for the environment and your budget:

• less material used for packaging

• lower package weight

• less fuel used for transportation

• lower shipping charges for you

Find out how much you can save when you don't have to pay for shipping the dry ice

4

qPCR Mixes: Specifications

HIGHLY COMPETITIVE

Four five-fold dilutions of 98 bp fragment of GAPDH gene were amplified from human genomic DNA using 5x HOT FIREPol® EvaGreen® qPCR Supermix (red) and qPCR mixes from companies A, B and C (dark blue, light blue and green). Reactions were per-formed on Applied Biosystems ViiATM 7 Real-Time PCR System following cycling pro-tocol recommended by the supplier.

1000

0010

000

∆Rn

Cycle20 22 24 26 28 30 32 34 36 38 40

Solis BioDyne Supermix Comp. A Comp. B Comp. C

Higher precision and wider scaleAll PCR and qPCR mixes from Solis BioDyne are produced as 5x mixes that occupy only one fifth of the reaction final volume leaving 2.5 times more room for the template compared with 2x mixes. This is highly advantageous when making wide-range serial dilutions or in case of gene expression studies where the concentration of the target gene is very low. Using the 5x mix enables the researcher to add more cDNA to the reaction which can result in better precision and lower Cycle threshold (Ct) values.

Light protective packagingDNA intercalating dye EvaGreen® and reference dye ROX necessary in many qPCR mixes are sensitive to light and therefore require protective shielding. Solis BioDyne packs all these qPCR mixes in special dark tubes to prevent harm from potential light exposure during transportation and reaction set-up.

Get 2,5x more reactions done with same volume of qPCR Mix

COMPARISON OF OUR 5X QPCR MIX TO STANDARD 2X QPCR MIX

Vial sizeReactions (20 µl

final volume)

2x Standard qPCR Mix 1 ml 100 rxn

5x Solis BioDyne qPCR Mix

1 ml 250 rxn

Quality ControlTo ensure highest quality and reproducible results in your qPCR assay, every product lot goes through strict quality control proce-dure including the following tests below:

• Amplification efficiency ≥ 105 fold• Functional quality control via qPCR on different templates• No ds Endodeoxyribonuclease activity• No ss Exodeoxyribonuclease activity• No self-priming activity

EvaGreen® dyeAll Solis BioDyne dye-based qPCR Mixes contain next- generation DNA-binding dye with features ideal for use in quantitative qPCR and HRM. EvaGreen® dye is non-toxic, non-mutagenic, and not hazardous to aquatic life.

• Highly sensitive – produces the most robust PCR signal when used at the recommended concentration

• Low PCR inhibition – exhibits much less PCR inhibi-tion than other detection dyes via a smart “release on demand” DNA-binding technology

• Extremely stable – simply indestructible under most biochemical conditions. Can be stored at ambient tem-perature and be subject to repeated freeze-thaw cycles

• Spectrally similar to other popular dyes – compatible with all major brand real-time thermal cyclers

• Environmentally safe – non-mutagenic, non-cytotoxic; safe to aquatic life for easy handling and disposal down the drain.


qPCR Mix Compatibility Table:Dye and Probe-based qPCR Mixes

Dye-based approachProbe-based

approach

5x H

OT

FIRE

Pol®

Eva

Gre

en®

qPCR

Sup

erm

ix

5x H

OT

FIRE

Pol®

Eva

Gre

en®

qPCR

Mix

Plu

s (R

OX)

5x H

OT

FIRE

Pol®

Eva

Gre

en®

qPCR

Mix

Plu

s (n

o RO

X)

5x H

OT

FIRE

Pol®

Eva

Gre

en®

qPCR

Mix

Plu

s (C

apill

ary)

5x H

OT

FIRE

Pol®

Eva

Gre

en®

HRM

Mix

(RO

X)

5x H

OT

FIRE

Pol®

Eva

Gre

en®

HRM

Mix

(no

RO

X)qPCR Platforms

5x H

OT

FIRE

Pol®

Pro

be q

PCR

Mix

Plu

s (R

OX)

5x H

OT

FIRE

Pol®

Pro

be q

PCR

Mix

Plu

s (n

o RO

X)

5x H

OT

FIRE

Pol®

Pro

be q

PCR

Mix

Plu

s (C

apill

ary)

5x H

OT

FIRE

Pol®

Pro

be

Uni

vers

al q

PCR

Mix

√ √ √

Applied Biosystems: 5700, 7000, 7300, 7700, 7900 HT, 7500, StepOne™, StepOnePlus™, ViiA™7, QuantStudio™ 3, 5, 6 Flex, 7 Flex, 12K Flex Real-Time PCR System

√ √

√ √Agilent/Stratagene: Mx3000P™, Mx3005P™, Mx4000PM

√ √

√ √ √BioRad: CFX96™, CFX384™, iQ™5, MyiQ™, Chromo4™, Opticon®2; MiniOpticon®

√ √

√ √ Cepheid®: SmartCycler® √ √

√ √ Eppendorf: Mastercycler® ep Realplex √ √

√ √ √Qiagen: Rotor-gene® 3000, Rotor-gene® 6000, Rotor-gene® Q

√ √

√ √ √ Thermo Scientific: PikoReal™ √ √

√ √ √ Illumina: The Eco™ √ √

√ √ √Roche Applied Science: LightCycler® 480, LightCycler® Nano, LightCycler® 96

√ √

√ Roche Applied Science: LightCycler® 1.x, 2.0

√

FIREPol is a trademark of Solis BioDyne. Applied Biosystems and StepOne are trademarks of Applied Biosystems LLC. QuantStudio and ViiA are trademarks of Life Technologies Corporation. Stratagene, Mx3000P, Mx3005P and Mx4000 are trademarks of Agilent Technologies inc. Mastercycler is a trademark of Eppendorf AG. Rotor-Gene is a trademark of Qiagen Group. Bio-Rad, CFX96, CFX384, iQ,MyiQ, Opticon 2, Chromo4, MiniOpticon are the trademarks of Bio-Rad Laboratories. Smartcycler and Cepheid are the trademarks of Cepheid Corporation. LightCycler and TaqMan are trademarks of Roche Molecular Systems Inc. The Eco is a trade-mark of Illumina Inc. PikoReal is a trademark of Thermo Fisher Scientific Inc. EvaGreen is a trademark of Biotium Inc. 3D model of FIREPol DNA Polymerase by Aare Abroi (Tartu University) and Sebastien Langui (Solis BioDyne), picture on the cover by Remo Savisaar.

6

5x HOT FIREPol® EvaGreen® qPCR Supermix

Send your sample request to [email protected]

PRODUCT CAT. NO. 20 UL REACTIONS SIZE/ml


08-36-0000S (free sample) 08-36-00001 08-36-00008 08-36-00020

5025020005000

0.21820

Researchers already trust Supermix

Reference:

“ I use Solis Supermix to analyse ChIP DNA and find it extremely reliable. Even with higher cycle numbers the replicates of the reaction give very stable results. We have also tested a qPCR mixture from another supplier and found that Supermix gives higher quality PCR products (single intensive band vs multiple bands of varying intensity). After that discovery we instantly switched to Solis mixture for all our qPCR reactions. ”SIGNE VÄRV PhD, post-doctoral researcher University of Bergen, Norway

Amplification plots of tenfold dilution series for the human GAPDH gene performed on Applied Biosystems™ ViiA™7 (upper graph) and Roche LightCycler® 480 (lower graph). The amount of DNA per reaction ranges from 0,01 to 10 ng. The results show high linear range and high efficiency across a wide range of DNA concentrations on different qPCR platforms.

Products and samples can be ordered via e-mail: [email protected], via skype: solis.biodyne, via phone: +372 740 9960, or via e-shop: www.sbd.ee

Selected publications:

• Urgard, E., et al. J. Control. Release (2016).

• Bednarek, K., et al. Tumor Biol. (2016).

• André, L., et al. Bioresource technology (2016).

• Inturi, R., et al. Virology (2015).

• Gajardo, T., et al. Immunology (2015).

DescriptionPrecisely optimized real time qPCR master mix based on DNA binding dye technology. This master mix has been developed to give highly specific and sensitive results.

Benefits• High sensitivity with low DNA concentrations• Reduced primer dimer formation• Reaction set-up and shipment without dry ice• One qPCR mix for all cyclers (except capillary)• Contains dUTP to prevent cross-contamination with UNG

treatment

Tip!EvaGreen dye is a

superior analogue to SYBR Green I dye.

You can keep using the same fluorescence

detection settings.

1E01

1E00

∆Rn

0.20.1

0.0118 20 22 24 26 28 30 32 34 36 38 40 42 44

Cycle Number

0,2Efficiency: 97.9 Slope: -3.37 R2:1

31.225

21.225

Fluo

resc

ence

(46

5-51

0)

11.225

1.225

18 20 22 24 26 28 30 32 34 36 38 40 42 44Cycle Number

Efficiency: 101,25Slope: -3.263

TRUSTWORTHY PERFORMANCE


Researchers already trust EvaGreen® Plus

Reference:

“ We have been using the 5X EvaGreen® Master Mix for a year. It works perfectly well for us! Compared with the products from other companies, its price is significantly lower and the quality of result is equally good or better! ”SUN, LEI PhD, Assistant Professor Cardiovascular & Metabolism Disorders Programme Supplied by TLC Scientific Solutions

10

0.1

0.2

0.0115

1

17 19 21 23 25 27 29 31 33N

orm

. flu

oro.

Cycle Number35

Excellent sensitivity and specificityThe amplification of 98 bp fragment of GAPDH gene exhibits sensitive and efficient reaction curves (upper graph) with highly specific peak in melt curve analysis (lower graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX). Amplification was performed on human genomic DNA using Rotor-Gene® 6000 qPCR cycler following cycling protocols recommended by the supplier.

66 70 74 78 82 86 90 94

dF /

dT

°C

2.8

2.4

2

1.6

1.2

0.8

0.4

0

EXCELLENT SENSITIVITY AND SPECIFICITY

The amplification of a 98 bp fragment of GAPDH gene exhibits sensitive and efficient reaction curves (upper graph) with highly specific peak in melt curve analysis (lower graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX). Amplification was performed on human genomic DNA using Rotor-Gene® 6000 qPCR cycler fol-lowing cycling protocols recommended by the supplier.


PRODUCT CAT. NO. RXN/20µl SIZE/ml

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

08-24-0000S (free sample)08-24-0000108-24-0000808-24-00020

5025020005000

0.21820

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)

08-25-0000S (free sample)08-25-0000108-25-0000808-25-00020

5025020005000

0.21820

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary)

08-26-0000S (free sample)08-26-0000108-26-0000808-26-00020

5025020005000

0.21820



• Krjutškov, K., et al. Scientific Reports (2016).

• Boija, A., Mannervik, M. Proceedings of the National Academy of

Sciences (2016).

• Mirzamohammadi, F., et al. Nature Communications (2016).

• Fliedl L., et al. New Biotechnology (2015).

• Anderson G.W., et al. Stem Cell Research & Therapy (2015).

DescriptionCost-effective real time qPCR master mix based on DNA binding dye technology. Provides highly reliable and reproducible results.

Benefits• High sensitivity and specificity• Excellent efficiency • Reaction set-up and shipment without dry ice• Master mixes for ROX, no ROX and capillary cyclers

5x HOT FIREPol® EvaGreen® qPCR Mix Plus

8

5x HOT FIREPol® EvaGreen® HRM Mix

DescriptionReliable and sensitive real time qPCR master mix for high resolution melt (HRM) analysis. This master mix uses the next-generation DNA binding dye - EvaGreen®.

Benefits• Reaction set-up and shipment without dry ice

• Mixes available for ROX and no ROX cyclers

• Sensitive EvaGreen® dye allows detection of DNA sequence variations

Researchers already trust EvaGreen® HRM Mix

Reference:

“ We appreciate your products because most of our research takes place in Kenya and it can be difficult to ship items that are temperature sensitive. The Hot Firepol® Evagreen® HRM Mix is very effective and the prices are reasonable. ”DR JEREMY HERREN International Centre of Insect Physiology and Ecology, Kenya

SENSITIVE HRM GENOTYPING

High Resolution Melt Analysis was used to genotype a C insertion in BRCA1 gene, a breast cancer suscepti- bility gene, with HOT FIREPol® EvaGreen® HRM Mix (two graphs above). Reactions were performed on Corbett Rotor-Gene® 6000. Green lines represent wildtypes with-out an insertion, red lines represent a C insertion and blue line represents a patient with unknown phenotype.



5x HOT FIREPol® EvaGreen®

HRM Mix (ROX)

08-33-0000S (free sample)08-33-0000108-33-0000808-33-00020

5025020005000

0.21820

5x HOT FIREPol® EvaGreen®

HRM Mix (no ROX)

08-31-0000S (free sample)08-31-0000108-31-0000808-31-00020

5025020005000

0.21820

90

70

50

30

10

77 78 79 80 81 8382

Nor

mal

ized

Flu

ores

cenc

e

°C

12

10

8

6

4

2

0

-277 78 79 80 81 82

Nor

mal

ized

min

us 5

382

mut

insC

°C83

No.

37

40414243

Name

Unknown phenotypeWildtype 1Wildtype 2Mutation 1Mutation 2

Genotype

5382 wt

5382 wt5382 wt5382 mut insC5382 mut insC

Confi. %

99,18

97,33100,00100,0097,47

Color


Did you know?High resolution melt

analysis can be used for SNP genotyping, discovering mutations, screening for heterozygosity, analysing

DNA methylation.


• Blouin, A. G., et al. Molecular Ecology

Resources (2016).

• Khori, V., et al. Lasers in Medical

Science (2016).

• Borecka, M., et al. Cancer Genetics (2016).

• Kim S.Y., et al. Journal of Microbiology

(2015).

• Rawluszko A.A., et al. BMC Cancer (2013).




5x HOT FIREPol® Probe Universal qPCR Mix

08-17-0000S (free sample)08-17-0000108-17-0000808-17-00020

5025020005000

0.21820


QPCR PERFORMANCE IN A DUPLEX REACTION:

Two genes from human gDNA were amplified in duplex reaction using HOT FIREPol® Probe Universal qPCR Mix. Excellent results were obtained from four 10x dilu-tions (starting from 10 ng/µl) from both genes. BAIP3 (blue) with GC-content 70,3% and efficiency 100% and GAPDH (yellow) with GC-content 56,1% and efficiency 98,4%. Reactions were performed on Applied Biosystems ViiATM 7 Real-Time PCR System.

12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44

1E00

0.01

Amplification Plot

∆Rn

CYCLE

HIGHLY COMPETITIVE QPCR MIX:

Four 10x dilutions of 197 bp long fragment of B4G4 gene with GC-content 75,6% were ampified from human gDNA using 5x HOT FIREPol® Probe Universal qPCR Mix (blue) and qPCR Mix from another vendor (green). Reactions were performed on Applied Biosystems ViiATM 7 Real-Time PCR System following cycling protocol recommended by each supplier.

Solis BioDyne Competitor

1.3

1.2

1.1

1.0

0.9

0.8

0.7

0.6

0.5

0.4

0.3

0.2

0.1

18 20 22 24 26 28 30 32 34 36 38 40 42 44

∆Rn

CYCLE

Researchers already trust Probe Universal

Reference:

“ We were using Firepol® Master Mix for our conventional PCR already and now we are developing Real-Time PCR and we were testing var-ious mixes. From the 3 different ones we tested, best results were with Solis Probe Universal qPCR Mix. ”ELENA IULIANA MACIUCA University of Liverpool, School of Veterinary Science

DescriptionPrecisely optimized real time qPCR master mix for probe based assays. This master mix has been developed for TaqMan® probes but is also suitable for other probe types such as Molecular Beacon and Scorpion.

Benefits• Suitable for singleplex and multiplex assays

• High specificity and sensitivity

• Reaction set-up and shipment without dry ice

• One qPCR mix for all cyclers (except capillary)

• Superior results with templates with up to 75% GC content

• Contains dUTP to prevent cross-contamination with UNG treatment


Tip!Probe based qPCR is recommended over a dye based approach when

specificity is especially important.

10



5x HOT FIREPol® Probe qPCR Mix Plus (ROX)

08-14-0000S (free sample)08-14-0000108-14-0000808-14-00020

5025020005000

0.21820

5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

08-15-0000S (free sample)08-15-0000108-15-0000808-15-00020

5025020005000

0.21820

5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)

08-16-0000S (free sample)08-16-0000108-16-0000808-16-00020

5025020005000

0.21820

10

0.1

0.0120

1

0.2

22 24 26 28 30 32 34 36 38 40

Del

ta R

n

Cycle Number

10

0.1

0.0120

1

0.2

22 24 26 28 30 32 34 36 38 40

Del

ta R

n

Cycle Number

10

0.1

0.0115

1

0.2

17 19 21 23 25 27 29 31 33

Del

ta R

n

Cycle Number35

10

0.1

0.0120

1

0.2

22 24 26 28 30 32 34 36 38 40

Del

ta R

n

Cycle Number

10

0.1

0.0120

1

0.2

22 24 26 28 30 32 34 36 38 40

Del

ta R

n

Cycle Number

10

0.1

0.0115

1

0.2

17 19 21 23 25 27 29 31 33D

elta

Rn

Cycle Number35

HIGHLY COMPETITIVE

Three tenfold dilutions of 72 bp fragment of Albumin gene were amplified from human genomic DNA using HOT FIREPol® Probe qPCR Mix Plus (red) and a qPCR mix from Company A (green). Reactions were performed on Applied Biosystems 7900HT Real-Time PCR System fol-lowing cycling protocols recommended by the supplier.

MULTIPLEX COMPATIBLE

Amplification of FAM labelled target SNAI1 (orange) and VIC labelled reference gene HPRT (yellow) was performed in a single reaction using HOT FIREPol® Probe qPCR Mix Plus. This multiplex qPCR was carried out on three ten-fold dilutions of human placental cDNA on Applied Bio-systems 7900HT Real-Time PCR System.

5x HOT FIREPol® Probe qPCR Mix Plus

DescriptionCost-effective real time qPCR master mix for probe based qPCR assays. This master mix has been developed for TaqMan® probes but is also suitable for other probe types such as Molecular Beacon and Scorpion.

Benefits• Suitable for singleplex and multiplex assays

• High specificity and sensitivity

• Reaction set-up and shipment without dry ice

• Master mixes for ROX, no ROX and capillary cyclers


• Vaht, M., et al. International Journal of Neuropsychop-

harmacology (2016).

• Bacakova, M., et al. International journal of nanomedi-

cine (2016).

• Bukovská, P., et al. Frontiers in microbiology (2016).

• Miller, M., et al. J Veterinar Sci Technol (2016).

Researchers already trust Probe Plus

Reference:

“ 5x HOT FIREPol® Probe qPCR Mix Plus (ROX) has been exten-sively used in our lab on a routine basis. Throughout the years, we have successfully analysed thousands of samples using this reliable, optimised, consumer- and budget-friendly solution provided by Solis BioDyne team. ”MARINA GRIGOROVA PhD, Research Fellow of Human Molecular Genetics University of Tartu



Product Selection Guide:Standard PCR Enzymes, Master Mixes and Other Enzymes

Standard PCR Enzymes and Master Mixes

HOT START FIDELITY VS TAQ READY TO LOADAMPLIFICATION

RANGECLONING TYPE ROBUST ON GC PAGE


√ 1× 3 kb TA * * 12

5x HOT FIREPol® GC Master Mix

√ 1× 3 kb TA * * * 13

5x HOT FIREPol® Blend Master Mix

√ 5× 5 kb Blunt * 14-15

5x HOT FIREPol® Blend Master Mix Ready to Load

√ 5× √ 5 kb Blunt * 14-15


1× 3 kb TA * * 16

5x FIREPol® Master Mix

1× 3 kb TA * 17

5x FIREPol® Master Mix Ready to Load

1× √ 3 kb TA * 17

Primer Extension

SPECIFICATION HOT START ddNTPs PAGE

HOT TERMIPolThermostable hot-start DNA Polymerase suited for MALDI-TOF and other primer extension platforms √ √ 18

TERMIPolThermostable DNA Polymerase suited for MALDI-TOF and other primer extension platforms √ 18

Reverse Transcription

SPECIFICATION

ECONOMICAL

CDNA

SYNTHESIS

RNA PROTECTION

FROM RNASE

DEGRADATION

OPTIMAL

WORKING

TEMP.

HIGH

SENSITIVITY

CDNA

COMPATIBLE

WITH QPCR

PAGE

M-MLV RNase H-Reverse Transcriptase RNase H- DNA Polymerase √ √ 37°C √ √ 19

12


Researchers already trust HOT FIREPol®

Reference:

“ We had some problems with the implementation of a protocol, we tried for a long time with different enzymes without any positive results. We tested the HOT FIREPol® and it was the perfect troubleshooting, besides the great technical support received from Solis BioDyne. ”DR MARIA JOSE SUAREZ CIHATA, University of Costa Rica


PRODUCT CAT. NO. SIZE/U

HOT FIREPol® DNA Polymerase (5 U/µl)

01-02-0000S (free sample)01-02-0050001-02-01000

1005001000

HIGHLY COMPETITIVE

Four genes from human gDNA were amplified in multi-plex reaction using HOT FIREPol® DNA Polymerase (lane 1-2) and two other hot start enzymes from company A (lane 3-4) and company B (lane 5-6). HOT FIREPol® DNA Polymerase performed well with all four genes in both 10x dilutions.

M 1 2 3 4 5 6

3041

bp

68%

2573

bp

69%

2002

bp

72%

1569

bp

72%

719

bp 7

8%Selected publications:

• Herrendorff, R., et al. Journal of Biological Chemistry

(2016).

• Keenan, J. I., et al. Anaerobe (2016).

• Małkiewicz, A., et al. Molecular Biology Reports (2016).

• Tiwari, A., et al. PloS one (2016).

• Kõks, G. et al. The American Journal of Pathology

(2015).


DescriptionChemically modified hot-start version of the thermostable Taq DNA polymerase FIREPol®. This enzyme is activated only after heat treatment which prevents any unspecific polymerase activity at lower temperatures during reaction set-up. HOT FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl

2 and an

additive for difficult templates.

Benefits• increased specificity and sensitivity

• reduced primer dimer formation

• reaction set-up and shipment without dry ice

M 1 2 3 4 5 6

3041

bp

68%

2573

bp

69%

2002

bp

72%

1569

bp

72%

719

bp 7

8%



Researchers already trust GC Master Mix

Reference:

“ Solis BioDyne’s highly flexible, customer-centered approach has proven to be invaluable when working on more challenging DNA regions. 5x HOT FIREPol® GC Master Mix is a high-quality and yet cost-effective solution for analyzing polymorphisms embedded in GC-rich regions. ”MARILIIS VAHT PhD student University of Tartu, Estonia




04-33-00S15 (free sample)04-33-0011504-33-02015

252505000

0.1 120

AMPLICONS OF VARIOUS GC-CONTENT

12 GC-rich genes were amplified from human gDNA using HOT FIREPol® GC Master Mix. Template DNA and DMSO were at final concentrations 1ng/µl and 10% respectively. The Master Mix performed well with GC-content up to 79%.

AMPLICONS OF VARIOUS LENGTHS FROM GC-RICH TEMPLATEGC-rich fragments of various length from human gDNA B4GN4 gene were amplified with HOT FIREPol® GC Master Mix. Template DNA and DMSO were at final concentrations 1ng/µl and 10% respectively. The Master Mix performed well up to 3000 bp in fragment length.

M 1 2 3 4 5 6 7 8 9 10 11 12

RET

392b

p 79

%

SOX1

8 79

8 bp

76%

HES

5 75

3bp

69%

ENST

733

bp 7

7%

PO3F

3 79

0bp

78%

Q8N

4 72

7bp

78%

B4G

N4

719b

p 78

%

Q4Z

IN3

763b

p 71

%

NP

758b

p 72

%

JUN

D 7

37bp

64,

6%

BAIP

3 78

8bp

65%

LMX1

737

bp 6

8%

M 1 2 3 4 5

3041

bp

68%

2573

bp

69%

2002

bp

72%

1569

bp

72%

719

bp 7

8%

Publication:

• Maksimov, M., et al. Neuropsychologia (2015).


Did you know?GC-rich templates need a special PCR master mix

because GC-rich DNA is more difficult to amplify.

It forms stable secondary structures that resist

denaturing and cause unspecific amplification.

DescriptionPCR master mix that has been developed for working with difficult GC-rich templates and DNA secondary structures. The master mix contains hot-start Taq polymerase HOT FIREPol®, MgCl

2, dNTPs and a special buffer for high yield GC-rich amplification.

Benefits• excellent amplification with templates up to 79% GC content• suitable for templates up to 3 kb• reaction set-up and shipment without dry ice• vials of 100% DMSO and 25 mM MgCl

2 allow flexibility with reaction

optimisation

14

5x HOT FIREPol® Blend Master Mix & Blend Master Mix Ready to Load

AMPLICONS OF VARIOUS LENGTH FROM DIFFERENT TEMPLATESLines 1-4 present an excellent amplification of frag-ments of various length from λ DNA. Lines 5 and 6 show two different amplicons amplified from mouse genomic DNA. All these reactions were carried out using 5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl

2.

M 1 2 3 4 5 6

Lane

123456

Template

λ DNA λ DNA λ DNA λ DNA Mouse ge mic DNA Mouse genomic DNA

Amplicon Length

499 bp1003 bp1998 bp4991 bp808 bp3838 bp

DescriptionPrecisely optimized PCR master mix for more demanding PCR assays. In addition to the hot-start Taq polymerase HOT FIREPol® this master mix contains a proofreading enzyme which offers enhanced performance.

Benefits• increased yield, sensitivity and specificity

• up to 5x higher fidelity

• longer templates (up to 5 kb)

• reduced primer dimer formation


Researchers already trust Blend

Reference:

“ After years of using the HOT FIREPol® for our routine colony PCR, we have switched to the Blend Master Mix Ready to Load. It still works perfectly and it is really time saving, especially since the loading dye is already included. ”CÉLINE WINCKLER Gene therapy and animal models for neurodegenerative diseases, Vision Institute, Paris, France


• Parker, N. R., et al. Scientific reports 6 (2016).

• del Mar Montiel-Rozas, M., et al. Mycorrhiza (2016)

• Kazantseva, J., et al. Scientific Reports 6 (2016).

• Proietti, P., et al. Agriculture, Ecosystems and Environment

(2015).

• Tedersoo L., et al. Science (2014).

Did you know?MgCl2 is needed for polymerase activity. Low MgCl2 concentration

can result in no PCR product, however too high MgCl2

concentration can increase unspecific amplification. The optimal concentration varies

within experiments but usually 1,5-2 mM is sufficient.




5x HOT FIREPol® Blend Master MixReady to Load with 7.5 mM MgCl

2

04-25-00S15 (free sample)04-25-0011504-25-02015

252505000

0.1120

5x HOT FIREPol® Blend Master MixReady to Load with 10 mM MgCl

2

04-25-00S20 (free sample)04-25-0012004-25-02020

252505000

0.1120

5x HOT FIREPol® Blend Master MixReady to Load with 12.5 mM MgCl

2

04-25-00S25 (free sample)04-25-0012504-25-02025

252505000

0.1120

5x HOT FIREPol® Blend Master MixReady to Load with 15 mM MgCl

2

04-25-00S30 (free sample)04-25-0013004-25-02030

252505000

0.1120



5x HOT FIREPol® Blend Master Mix with 7.5 mM MgCl

2

04-27-00S15 (free sample)04-27-0011504-27-02015

252505000

0.1120

5x HOT FIREPol® Blend Master Mix with 10mM MgCl

2

04-27-00S20 (free sample)04-27-0012004-27-02020

252505000

0.1120


2

04-27-00S25 (free sample)04-27-0012504-27-02025

252505000

0.1120

5x HOT FIREPol® Blend Master Mix with 15 mM MgCl

2

04-27-00S30 (free sample)04-27-0013004-27-02030

252505000

0.1120


16


MOUSE GENOMIC DNA

1200 bp fragment of Beta-synuclein gene was amplified from mouse genomic DNA using FIREPol® DNA Polymerase with two different buffers: B (lane 1-3) and BD (lane 4-6). Template DNA was serially diluted tenfold with a starting concentration of 1 ng/µl. FIREPol® DNA Polymerase was used at 0.04U/µl.

PLANT GENOMIC DNA

672 bp fragments was amplified from barley genomic DNA using FIREPol® DNA Polymerase with two buffers: B (lane 1-3) and BD (lane 4-6). Template DNA was serially diluted tenfold with a starting concentration of 1 ng/µl. The enzyme performed well even with the template’s concentration being as low as 0.01 ng/µl. FIREPol® DNA Polymerase was used at 0.04 U/µl.



FIREPol® DNA Polymerase (5 U/µl)

01-01-0000S (free sample)01-01-0050001-01-0100001-01-02000

10050010002000

M 1 2 3 4 5 6

M 1 2 3 4 5 6

M 1 2 3 4 5 6

M 1 2 3 4 5 6

Researchers already trust FIREPol®

Reference:

“ I found that for FIREPol® DNA Polymerase the quality was comparable to similar products even though the price was much cheaper for the Solis product. Therefore, Solis BioDyne are head and shoulders above their competitors when it comes to value for money which is especially important given the funding situation in these straitened times. ”DR. GARY LOUGHRAN Research Fellow School of Biochemistry and Cell Biology University College Cork, Ireland


• Corte, L., et al. Scientific Reports (2016).

• Lawrence, S. A., and Poulin, R., Parasitology

Research (2016).

• Pilkington, K. M., and V. Vaughan Symonds. Applications

in Plant Sciences (2016).

• Tockner, B., et al. Gene Therapy (2016).

• Jopcik, M., et al. Molecular Biology (2015).

• Corte, L., et al. Food Microbiology (2015).

DescriptionGenetically modified thermostable Taq DNA polymerase that pro-vides robust and reproducible results. FIREPol® DNA polymerase is supplied with 2 reaction buffers, 25 mM MgCl

2 and an additive for

difficult templates.

Benefits• robust amplification for routine applications

• suitable for templates up to 3 kb




Researchers already trust FIREPol® Master Mix Reference:

“ We are working with Firepol® Mastermix for few years now and we are very satisfied. We are using it for genotyping (many different PCR) and we have excellent results. The ready to load format is very time saving. ”CLAUDINE CORNELOUP Engineer assistant Platform for Experimental Biology on Mice, Genotyping Laboratory, ENS de Lyon

5x FIREPol® Master Mix & Master Mix Ready to Load

DescriptionOptimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl

2, dNTPs and buffer with detergent. You just

need to add template, primers and water.

Benefits• ready to load version allows you to load the PCR product on your gel straight after cycling


• all-in-one master mix format reduces pipetting errors and saves time



5x FIREPol® Master Mix with 7.5 mM MgCl2

04-11-00S15 (free sample)04-11-00115

25250

0.11

5x FIREPol® Master Mix with 12.5 mM MgCl2 04-11-00S25 (free sample)

04-11-0012525250

0.11

5x FIREPol® Master Mix Ready to Load with 7.5 mM MgCl

2

04-12-00S15 (free sample)04-12-00115

25250

0.11

5x FIREPol® Master Mix Ready to Load with 12.5 mM MgCl

2

04-12-00S25 (free sample)04-12-00125

25250

0.11

PLANT GENOMIC DNA

672 bp fragment was amplified from barley genomic DNA using FIREPol Master Mix (lane 1-3) and FIREPol Master Mix Ready to Load (lane 4-6). Template DNA was serially diluted tenfold from a starting concentration of 1 ng/µl. The Master Mixes performed well even with the template’s concentration being as low as 0.01 ng/µl.

M 1 2 3 4 5 6


• Mohammadi, S. M., et al. Neuropeptides

(2016).

• Pooljun, C., et al. Aquaculture (2016).

• Purahong, W., et al. Fungal Ecology (2016).

• Trüeb, B., et al. Veterinary Microbiology

(2016).

• Al-Mohaya, M.A.M., et al. BMC Oral Health

(2016).

• Rasheed, F., et al. Heliobacter (2014).


18

Researchers already trust TERMIPol® Reference:

“ Our group is using the TERMIPol® already for 10 years for primer extension

reactions with subsequent HPLC separation. Compared to similar products on

the market TERMIPol® incorporates ddNTPs with high efficiency and low error

rates. We highly recommend using this enzyme for SNP genotyping or bisulfite-

based single CpG screening, as low as 1.25 U are sufficient per reaction. Since no

detergents are used in storage and reaction buffers, primer extension reactions

can be loaded unpurified on HPLC systems which saves time and costs. We are

using this enzyme frequently and experienced TERMIPol® as robust and reliable

enzyme offering highly efficient and reproducible results. ”DR. SASCHA TIERLING Universität des Saarlandes, GERMANY



TERMIPol® DNA Polymerase (5 U/µl)

01-03-0000S (free sample)01-03-0050001-03-02000

5005002000

HOT TERMIPol® DNA Polymerase (5 U/µl)

01-06-0000S (free sample)01-06-0050001-06-02000

5005002000

TERMIPol® DNA Polymerase

DescriptionThermostable DNA polymerase that has an increased efficiency for incorpo-rating unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides. TERMIPol® DNA Polymerase is supplied with a reaction buffer and 100 mM MgCl

2.

Benefits• high efficiency for incorporating unconventional nucleotides

• assay success rate of 99% in MALDI-TOF

• robust and reliable



• Royo, J.L., et al. Molecular and Cellular Probes (2015).

• Gorokhova, S.G., et al. J. Clin Exp Cardiolog (2014).

• Thorkildsen, L.T., et al. Gastroenterology Research and

Practice (2013).

• Ilina, E.N., et al. Front Microbiol (2013).

• Tierling, S., et al. International Journal of Cancer (2012).

Did you know?The ability to incorporate

unconventional nucleotides makes TERMIPol suitable for

primer extension, MassARRAY and MALDI-TOF mass

spectrometry.



M-MLV Reverse Transcriptase RNase H- DNA Polymerase

DescriptionGenetically modified M-MLV reverse transcriptase with RNA or DNA dependent DNA polymerase activity but lacking RNase H activity. The optimal reaction temperature for this enzyme is 37oC. The kit contains M-MLV RT RNase H-, 2 buffers, vials of 25 mM MgCl

2, 20 mM

MnCl2 and 100 mM DTT.

Benefits• Cost-effective enzyme for cDNA synthesis

• cDNA is compatible with downstream qPCR applications

• No RNase H activity

cDNA

RNase

RNase H degrades mRNA, causing truncation of cDNA

mRNAA

T

A

T

A

T

ReverseTranscriptase

cDNA

mRNAA

T

A

T

A

T

ReverseTranscriptase

Removal of the RNase H activity results in an increase of full-length cDNA products

RNASE H ACTIVITY

Researchers already trust M-MLV H- Reference:

“ Our research group has been using Solis BioDyne's M-MLV Reverse Transcriptase in gene expression analysis by RT-qPCR for many years. The enzyme yields reproducible results with high sensitivity and is affordably priced. ”TÕNIS ÖRD PhD, senior research scientist Estonian Biocentre


• Giordani, T., et al. Tree Genetics & Genomes (2016).

• Raba, M., et al. Frontiers in Cellular Neuroscience

(2016).

• Wen, B., et al. Plos ONE (2014).

• Örd, T., et al. Cellular Immunology (2012).

• Kesireddy, V., et al. Molecular Biology Reports (2010).



M-MLV Reverse Transcriptase RNase H- (200 U/µl)

06-21-00000S (free sample)06-21-01000006-21-050000

20001000050000


20

dNTP Mix and Set

dATPFormula: C

10H

12N

5O

12P

3 (Anion)

Formula Weight: 487.18 (Anion)

dGTPFormula: C

10H

12N

5O

13P

3 (Anion)


dTTPFormula: C

10H

12N

2O

14P

3 (Anion)


dCTPFormula: C

9H

12N

3O

13P

3 (Anion)


O

O

OO-O-

O O

O

O

N

N NP P P

O OO- O-

NH

OH

NH2

O

O

OO-O-

O O

P P PO O

O- O- OON

N

NH2

OH

O

OO-O-

O

O

OO

P P PO O

O- O-

O

N

OH

NHH

3C

O

O

OO-O-

O O

O

N

N N

NP P P

O OO- O-

OH

NH2


PRODUCT CAT. NO. SIZE/µmol

dNTP Set02-21-0001S (free sample)02-21-0010002-21-00400

4x14x254x100

dNTP Mix02-31-0001S (free sample)02-31-0002002-31-00100

0.820100

dUTP02-41-0000S (free sample)02-41-00025

2.525

“ In 2005 we started to use the Solis Biodyne dNTP Set in our lab. Comparing the performance of Solis Biodyne dNTPs with two other suppliers in a mutation detection assay, we found similar or even higher FRET signals in our analysed samples. Since then, we use the Solis Biodyne dNTP Set in our lab in a wide range of DNA and RNA amplification techniques like end point PCR, mutation detection in FRET assays, qPCR, high resolution melting analysis etc. ”JUERGEN SIEVERTSEN Bernhard Nocht Institute for Tropical Medicine (BNITM), Germany

DescriptionSolis BioDyne's dNTPs are chemically synthesised and have 99% purity determined by HPLC. You can use our dNTPs for a wide range of molecular biology applications.

dNTP SetSeparate vials of dATP, dTTP, dGTP and dCTP at 100 mM concentration.

dNTP MixOne solution of dATP, dTTP, dGTP and dCTP at 20 mM concentration each.

dUTPdUTP is available in a separate vial with a concentration of 100 mM.



100 bp DNA Ladder 1 kb DNA Ladder


PRODUCT CAT. NO. SIZE

100 bp DNA LadderReady to Load

07-11-0000S (free sample)07-11-00050

1.5 µg50 µg

1 kb DNA LadderReady to Load

07-12-0000S (free sample)07-12-00050

1.5 µg50 µg

6x DNA Loading Dye Buffer Blue

07-01-0000S (free sample)07-01-0000107-01-00010

0.1 ml1 ml10 ml

6x DNA Loading Dye Buffer Double Blue

07-02-0000S (free sample)07-02-0000107-02-00010

0.1 ml1 ml10 ml

6x DNA Loading Dye Buffer Orange and Blue

07-03-0000S (free sample)07-03-0000107-03-00010

0.1 ml1 ml10 ml

6x DNA Loading Dye Buffer Orange

07-04-0000S (free sample)07-04-0000107-04-00010

0.1 ml1 ml10 ml

1 KB DNA LADDER 100 BP DNA LADDER

Lane

1234

DNA Loading Dye Buffer

BlueDouble BlueOrange and BlueOrange

1 2 3 4

Lane

1234

DNA Loading Dye Buffer

BlueDouble BlueOrange and BlueOrange

1 2 3 4 LOADING DYE BUFFERS

In 1% agarose gel 1x TBE, Xylene Cyanol FF migrates along with~3500 bp frag-ments, Bromophenol Blue migrates along with ~300 bp fragments and Orange G migrates along with ~40 bp fragments.

-10,000 80

-3,000 120

-5,000 80

bp ng/10µl

-8,000 80

-4,000 80

-2,500 123

-2,000 81

-1,500 73

-1,000 41

-700 57

-500 61

-300 44

-3,000 100

bp ng/10µl

-2,000 134

-1,500 100

-1,000 104

-800 83-900 93

-700 73-600 63-500 104-400 42-300 31

-200 42

-100 31

1 kb DNA Ladder 100 bp DNA Ladder

6x DNA Loading Dye Buffers

Description6x DNA Loading Dye Buffers are used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. The optimized solutions contain different mixtures of three dyes: Bromophenol Blue, Xylene Cyanol FF and Orange G for visual tracking of DNA migration during electrophoresis.

6x DNA Loading Dye Buffers containing Orange G are recommended for the analysis of small DNA molecules and have no DNA masking during gel exposure to UV light. 6x DNA Loading Dye Buffer Blue and Dye Buffer Double Blue make pipetting visually easy with its dark blue color.

DescriptionSolis BioDyne DNA ladders are convenient ready-to-use molecular weight markers for DNA fragment size determination on gel electrophoresis. The ladders are supplied in a loading buffer and are stable at ambient temperature. The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging from 300 bp to 10,000 bp. The 100 bp DNA Ladder contains 13 discrete DNA fragments ranging from 100 bp to 3,000 bp.


22


PRODUCT CAT. NO. SIZE/ml

10x GC-rich Enhancer

05-16-0000S (free sample)05-16-0001005-16-0005005-16-00200

0.11520

25 mM MgCl2

05-11-0002505-11-0005005-11-00200

2.5520

PCR Grade Waterwater-025water-100water-500

25100500

10x GC-rich EnhancerDescription10x GC-rich Enhancer is used as PCR additive for difficult GC-rich templates. The optimized solution modifies melting behavior of nucleic acids and often enhances amplification of suboptimal PCR systems with high degree of secondary structures and GC-rich regions.

10x GC-rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non-specific amplification occurs.

Applications• Additive for PCR reaction

25 mM MgCl2

DescriptionMagnesium Chloride (MgCl

2) is an important component of PCR reactions. Concentration

of MgCl2 should be optimized according to reaction conditions (primer, template, dNTP,

polymerase concentration).

Applications• Optimization of PCR, qPCR and RT-PCR reactions• All other molecular biology techniques where MgCl

2 is needed

PCR Grade WaterDescriptionPCR Grade Water is deionized and autoclaved water suitable for use in all experiments that require nuclease-free water. PCR Grade Water is prepared without chemical additives and it is DNase, RNase and nuclease-free.

Applications• PCR, qPCR and RT-PCR • All other molecular biology techniques where pure water is needed



How to OrderOrders can be placed:

• Via E-Shop: www.sbd.ee• By emailing: [email protected]• With your account manager or local distributor• Via fax: +372 740 2079

Required Information

Following information is required while placing an order:

• Shipping and invoice address• Contact person's name and phone number• VAT number (EU only)• Product name and corresponding catalogue number

Shipping Cost

Depending on the order amount a shipping cost may be added to the invoice. Please contact us for shipping cost quotation.

Customer Care

We are committed to providing our customers excellent service. All inquiries will be responded to within 1 business day at most. All technical questions will be given high priority and our full attention.

Please contact us through Skype: support.sbd or via e-mail: [email protected]

Customized solutions

This product catalogue contains standard products, tube sizes and kits. Please contact us for customized solutions.

Shipping

Unless agreed otherwise, all shipments abroad will be arranged via express courier service. Orders are confirmed generally within 1 business day (Monday to Friday, 8AM to 5PM, UTC+2) after reception. In most cases orders are shipped within 1 or 2 business days.

Delivery documents and other charges

For non-EU shipments, please inform us of the documents required for shipments to your country. Solis BioDyne is not liable for import duties and taxes or delays caused by the brokerage procedure or other third parties.

Payment Options

Solis BioDyne accepts payments by:

• Wire transfer, based on invoice• PayPal, based on invoice or for orders placed through e-shop• Credit Card (VISA or Master Card) for orders placed through

our e-shop

Checks are not accepted as a payment method.

Please see our full ordering conditions on www.sbd.ee

All Solis BioDyne products are shipped at ambient temperature.

Our products can withstand ambient temperature up to 1 month without any loss of activity. However, regular storage at -20°C is recommended.

Ordering

Free samplesSolis BioDyne provides free samples of the entire product range enabling our clients to thoroughly test our products.

24

We are based in Estonia, a member of the European Union and €-zone

International presence

International PresenceSolis BioDyne currently has clients in 110 countries. We make our reagents accessible globally either by supplying them directly or in some areas by relying on local distributors who share our high standards in service and technical support. Please see the distributors section on next page or contact us directly to find the most convenient way to order in your region.


DistributorsAFRICA

DELTA Trading & Development Egypt T: +20 2 3573 6679 E: [email protected] www.deltatd.com

SeparationsSouth AfricaT: +27 11 919 1000 E: [email protected]

ASIA

Biogenuix Medsystems Pvt. Ltd. India T: +91 11 2561 2008E: [email protected] www.biogenuix.com

PT. Sentra Biosains DinamikaIndonesia T: +62 21 4288 6574 E: [email protected] www.sentrabd.com

Cosmo Bio Co., Ltd.JapanP: +813 5632 9610E: [email protected]

Next Gene ScientificMalaysiaT: +603 5882 8650E: [email protected] www.nextgene.my

Med Lab ServicesPakistanT: +92 51 4419341 E: [email protected]

Genomicbase Republic of Korea T: +82 2 2215 4925E: [email protected]

TLC Scientific Solutions Pte. Ltd.SingaporeT: +656464 0261E: [email protected]

Hongblue Life Science Trade Co. Taiwan T: +886 4 2263 1262E: [email protected] www.hongblue.url.tw

Life Science AP Co., LtdThailandT: +66 81 8296282E: [email protected]

EUROPE

Medibena Austria T: +43 1 9906 497 E: [email protected] www.medibena.at

Laborimpex SABelgiumT: +32 2 345 99 94E: [email protected]

BariaCzech Republic T: +420 774 227 421 F: +420 244 911 2 28 E: [email protected] www.baria.cz

TAG Copenhagen A/SDenmarkT: +45 321 322 00 E: [email protected]

LabnetFinland T: +358 20 741 31 70 F: +358 20 741 31 89 E: [email protected] www.labnet.fi

BioinnotechGreeceT: +30 210 51 41 746www.bioinnotech.gr

Bio-KasztelHungaryT: +36 1 381 0694E: [email protected] www.kasztel.hu

Euroclone S.P.AItalyT: +39 02 38.19.51E: [email protected]

SIA AdronaLatviaT: +371 67551894E: [email protected]

Bio-Connect B.V.The NetherlandsT: +31 26 3264 450E: [email protected]

Cytogen Polska Sp. z o.o. Poland T: +48 42 6300 598 E: [email protected] www.cytogen.com.pl

DAGMA, Lda. Portugal T: +351 21 4573 222 E: [email protected]

Bio Zyme SRLRomaniaT: +40 264 523281E: [email protected]

Ecoli s.r.o. SlovakiaT: +421 0264 789 336 E: [email protected] www.ecoli.sk

Genycell BiotechSpainT: +34 902 194353E: [email protected]

LucernaChem AGSwitzerlandT: +41 41 4209 636E: [email protected]

BM Yazılım Danıs. ve Laboratory Systems Ltd. Sti. Turkey T: +90 312 4472 280 E: [email protected] www.bmlabosis.com

Newmarket Scientific United Kingdom T: +44 (0)1638 551500 E: [email protected] www.newmarketscientific.com

Tespro LLCUkraineT: +380 44 220 110 5E: [email protected]

OCEANIA

Integrated Sciences Pty. Ltd. AustraliaT: +61 2 9417 7866 E: [email protected] www.integratedsci.com.au

dnatureNew ZealandT (toll-free): 0800 362 8873E: [email protected]

MIDDLE EAST

BioConsult Israel T: +972 2 5667 043 E: [email protected] www.bioconsult.co.il

Pishgam Biotech CompanyIranT: +98 21 8801 4393E: [email protected]

Al Genome Medical CompanyJordanT: + 962 652 33 670E: [email protected]

Al Genome International Scientific and Laboratory ProductsKingdom of Saudi ArabiaUnited Arab Emirates T: +971557011398E: [email protected]

Med CloneKuwaitT: +965 23915602E: [email protected]

NORTH AMERICA

Mango Biotechnology LLC USA T: +1 650 5758 657 E: [email protected] www.mangobio.com

SOUTH AMERICA

Biocientifica S.A. ArgentinaT: (54-11) 4857-5005E: [email protected]

Sinapse Biotecnologia LtdaBrazilT: +55-11-2605-5655E: sinapse@sinapsebiotecnologia.com.brwww.sinapsebiotecnologia.com.br

Fermelo Biotec Chile T/F: +562 2247 2978E: [email protected]

SurGenoma S.A.S. ColombiaT: +57 1 3441325E: [email protected]

The most up-to-date list of distributors is available on our website www.sbd.eei

26

qPCR Mixes

CAT. NO. SIZE


08-36-0000S08-36-0000108-36-0000808-36-00020

50 rxn/20 µl250 rxn/20 µl

2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)

08-24-0000S08-24-0000108-24-0000808-24-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX)

08-25-0000S08-25-0000108-25-0000808-25-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary)

08-26-0000S08-26-0000108-26-0000808-26-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® EvaGreen® HRM Mix (ROX)

08-33-0000S08-33-0000108-33-0000808-33-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® EvaGreen® HRM Mix (no ROX)

08-31-0000S08-31-0000108-31-0000808-31-00020


2000 rxn/20 µl5000 rxn/20 µl


08-17-0000S08-17-0000108-17-0000808-17-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPolR Probe qPCR Mix Plus (ROX)

08-14-0000S08-14-0000108-14-0000808-14-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® Probe qPCR Mix Plus (no ROX)

08-15-0000S08-15-0000108-15-0000808-15-00020


2000 rxn/20 µl5000 rxn/20 µl

5x HOT FIREPol® Probe qPCR Mix Plus (Capillary)

08-16-0000S08-16-0000108-16-0000808-16-00020


2000 rxn/20 µl5000 rxn/20 µl

Regular PCR Enzyme and Mixes

CAT. NO. SIZE

FIREPol® DNA Polymerase (5 U/µl)

01-01-0000S 01-01-00500 01-01-01000 01-01-02000

100 U 500 U

1000 U 2000 U

5x FIREPol® Master Mix with 7,5 mM MgCl2

04-11-00S15 04-11-00115

25 rxn/20 µl 250 rxn/20 µl

5x FIREPol® Master Mix with 12.5 mM MgCl2

04-11-00S25 04-11-00125

25 rxn/20 µl 250 rxn/20 µl

5x FIREPol® Master Mix Ready to Load with 7,5 mM MgCl2

04-12-00S15 04-12-00115

25 rxn/20 µl 250 rxn/20 µl

5x FIREPol® Master Mix Ready to Load with 12,5 mM MgCl2

04-12-00S25 04-12-00125

25rxn/20 µl 250 rxn/20 µl

Other Enzymes

CAT. NO. SIZE

TERMIPol® DNA Polymerase (5 U/µl) 01-03-0000S01-03-0050001-03-02000

500 U500 U

2000 U

HOT TERMIPol® DNA Polymerase (5 U/µl) 01-06-0000S01-06-0050001-06-02000

500 U500 U

2000 U

M-MLV Reverse Transcriptase RNase H- (200 U/µl) 06-21-00000S06-21-01000006-21-050000

2000 U10000 U50000 U

Product List


Hot-start PCR Enzyme and Mixes

CAT. NO. SIZE

HOT FIREPol DNA Polymerase (5U/µl)01-02-0000S01-02-0050001-02-01000

100 U500 U

1000 U

5x HOT FIREPol® GC Master Mix 04-33-00S1504-33-0011504-33-02015


5000 rxn/20 µl


2

04-27-00S1504-27-0011504-27-02015


5000 rxn/20 µl


2

04-27-00S2004-27-0012004-27-02020


5000 rxn/20 µl


2

04-27-00S2504-27-0012504-27-02025


5000 rxn/20 µl


2

04-27-00S3004-27-0013004-27-02030


5000 rxn/20 µl

5x HOT FIREPol® Blend Master Mix Ready to Load with 7.5 mM MgCl

2

04-25-00S1504-25-0011504-25-02015


5000 rxn/20 µl

5x HOT FIREPol® Blend Master Mix Ready to Load with 10 mM MgCl

2

04-25-00S2004-25-0012004-25-02020


5000 rxn/20 µl

5x HOT FIREPol® Blend Master Mix Ready to Load with 12.5 mM MgCl

2

04-25-00S2504-25-0012504-25-02025


5000 rxn/20 µl

5x HOT FIREPol® Blend Master Mix Ready to Load with 15 mM MgCl

2

04-25-00S3004-25-0013004-25-02030


5000 rxn/20 µl

Other PCR Reagents

CAT. NO. SIZE

dNTP Set02-21-0001S 02-21-00100 02-21-00400

4x1 µmol 4x25 µmol

4x100 µmol

dNTP Mix02-31-0001S 02-31-00020 02-31-00100

0.8 µmol 20 µmol

100 µmol

dUTP 02-41-0000S 02-41-00025

2.5 µmol 25 µmol

100 bp DNA Ladder Ready to Load 07-11-0000S 07-11-00050

1.5 µg 50 µg

1 kb DNA Ladder Ready to Load07-12-0000S 07-12-00050

1.5 µg 50 µg

6x DNA Loading Dye Buffer Blue07-01-0000S 07-01-00001 07-01-00010

0.1 ml 1 ml

10 ml

6x DNA Loading Dye Buffer Double Blue07-02-0000S 07-02-00001 07-02-00010

0.1 ml 1 ml

10 ml

6x DNA Loading Dye Buffer Orange and Blue07-03-0000S 07-03-00001 07-03-00010

0.1 ml 1 ml

10 ml

6x DNA Loading Dye Buffer Orange07-04-0000S 07-04-00001 07-04-00010

0.1 ml 1 ml

10 ml

10x GC-rich Enhancer

05-16-0000S 05-16-00010 05-16-00050 05-16-00200

0.1 ml 1 ml 5 ml

20 ml

25 mM MgCl2

05-11-00025 05-11-00050 05-11-00200

2.5 ml 5 ml

20 ml

PCR Grade Waterwater-025water-100water-500

25 ml100 ml500 ml

Solis BioDyne

Riia 185a, 51014 Tartu, EstoniaTel: +372 7409 960Fax: +372 7402 079E-mail: [email protected]: solis.biodyne

VAT No: EE100587614Reg. No: 10242922

Bank details:Swedbank ASIBAN code: EE692200221005142234SWIFT/BIC.: HABAEE2XBank address: Liivalaia 8, 15040 Tallinn, Estonia

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olis

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