•Mycobacteria Growth Indicator Tube• Modified Middlebrook 7H9 broth base• Detection of growth through detection of O2

consumption• Fluorescent sensor responds to O2 concentration• Mycobacteria are aerobic organisms and will utilize

O2 at readily detectable rates

WHAT IS MGIT ?

• Menggunakan MGIT media dan patented sensors, menjadikan lebih efisien dengan penggunaan teknologi fluorometri.• Deteksi yang akurat pada

pertumbuhan bakteri dengan cara mendeteksi konsumsi O2 oleh bakteri.

• Automated instrument quality control• Performed continuously to ensure

precise and reliable operation.• Specimen results provided as

Positive or Negative.

BACTEC® MGIT™ 960

MGIT Technology indicator system

tris-4, 7-diphenyl-1, 10-phenanthroline ruthenium dichloride pentahydrate-DPPRDP

Negative Culture

CO2

O2O2

O2 O2

O2

O2

Vial Headspace

Growth Medium

Sensor

Little or No Fluorescence

FF F

FFO2

FO2

F

F

F F

CO2

O2

O2

O2

VialHeadspace

Growth Medium

Sensor

Positive Culture

Strong Fluorescence

FO2FO2

FO2

FO2FO2

F

F

CO2

CO2

CO2

105-106 CFU/ml

10% CO2

• Detector Board located in each drawer.• 16 individual detection

stations.• Data collected from each tube

once each hour.• Data evaluated and tube

positivity established based on software algorithms.

DETECTION SYSTEM

• Indicator lamp on drawer illuminates.• Audible alert sounds.

NOTIFICATION OF POSITIVES