principles of antimicrobial therapy part 1
TRANSCRIPT
Principles of Principles of Antimicrobial Antimicrobial
TherapyTherapyPart 1Part 1
Principles of Principles of Antimicrobial Antimicrobial
TherapyTherapyPart 1Part 1
Principles of Antimicrobial
Therapy
• Effective in the treatment of infections ( selective toxicity)
• Ability to kill microorganism without harming the cells of the host
MicroorganismsSource of Infection
• Bacteria• Viruses• Fungi
Topics• Antibacterial agents• AntiTB drugs• Antifungal• Antiviral• UTI
Chain of Infection•
Susceptibility of the body to infection
• 1. Age• 2. Exposure to pathologic organisms• 3. Disruption of the body’s normal
barrier to infection• 4. Impaired Immune system• 5. Impaired circulation• 6. Poor nutritional status
Selection of Antimicrobial
Agents:• Organism’s identity and its sensitivity• Site of infection• Age of the patient• Pregnancy / Lactation• Safety of the agent• Patient factors• Cost of therapy
Empiric Therapy
• immediate administration of drug/s covering by both gram-positive and gram-negative microorganisms
Empiric Therapy • A. Empiric therapy prior to organism identification• - The acutely ill patient• - Selecting a drug – site of infection and patient history
• B. Identification and sensitivity of the organism• - Gram stain• - Culture• - Microscopic examination• - Sensitivity testing
• C. Laboratory methods of identification• - Disk-diffusion
Empiric Therapy• D. The effect of the site of
Infection on therapy
• - Blood brain barrier• - Prostate – pH of prostatic fluid
6.4 and plasma is 7.4
Empiric TherapyA. Status of the Patient
• 1.Immune system• - Alcoholism, DM HIV, malnutrition,
advanced age and immunosuppressive drugs
• 2.Renal dysfunction• - Poor kidney function 10% or less of normal• Serum creatinine
Empiric Therapy• Status of the Patient
• 3.Hepatic dysfunction• - Erythromycin and tetracycline
• 4.Poor perfusion• - DM – decreased circulation to an
anatomic area
Empiric Therapy• 5. Pregnancy
• ALL ANTIBIOTICS CROSS THE PLACENTA• Adverse effect are rare • Tooth dysplasia and inhibition of bone growth
(tetracycline)• Antihelmintics – embryotoxic and teratogenic• Aminoglycosides – ototoxic• Streptomycin – auditory nerve damage
Empiric Therapy• 6. Lactation• drugs administered to a lactating mother may
enter the nursing infant via the breast milk
• 7. Age• Renal and hepatic elimination ( Newborns)• Chloramphenicol and sulfonamides• Tetracycline – bone growth• Fluoroquinolones – cartilage growth
Empiric Therapy• 8. Safety of the Agent• Penicillin the least toxic of all drugs
• SAFETY IS RELATED NOT ONLY TO THE INHERENT NATURE OF THE DRUG BUT ALSO TO PATIENTS FACTORS THAT CAN PREDISPOSE TO TOXICITY
• • G. Cost of Therapy
Bacteriostatic drugs
- arrest the growth and replication of bacteria at serum levels achievable in the patient, thus limiting the spread of infection while the body’s immune system attacks, immobilizes, and eliminates the pathogen
- tetracyclines, erythromycin, lincomycin
Bactericidal drugs • kills the bacteria and the total number of
viable organisms decreases
• e.g. Chloramphenicol – static for gram negative and cidal against Pneumococci
• Cephalosporins, Polymyxin, vancomycin
Chemotherapeutic Spectra
• refers to the species of organisms affected by certain drug
• 1. Narrow spectrum – single or a limited group of microorganisms
• E.g Isoniazid
Chemotherapeutic Spectra
• b. Extended Spectrum – antibiotics that are effective against
gram-positive and gram-negative
• E.g. Ampicillin
Chemotherapeutic Spectra
• 3. Broad Spectrum – wide coverage, drastically alter the
nature of the normal bacterial flora and can precipitate a superinfection of the organism (Candida)
• E.g. Tetracycline and Chloramphenicol
Combinations of Antimicrobial Drugs
• 1. Advantages of drug combinations• - B-lactams and aminoglycosides
• 2. Disadvantages of drug combinations• - A number of antibiotics act only when
organisms are growing. Concomitant administration of a second agent is usually bacteriostasis and may interfere with the action of the first drug that is bactericidal
Drug Resistance• 1. Genetic alterations leading to drug
resistance• -Spontaneous mutation of DNA• - DNA transfer of drug resistance
• 2. Altered expression of proteins in drug-resistant organisms
• - Modification of target sites• - Decreased accumulation• - Enzymatic inactivation
Antibiotic therapy• Prophylactic Antibiotics
– Before and after exposure to a disease entity
Complications of Antibiotic Therapy
• 1. Hypersensitivity - Penicillin
• 2. Direct toxicity / Organ toxicity• - Aminoglycosides• - Chloramphenicol – Aplastic anemia
• 3. Superinfections • – broad-spectrum
Classification of Antimicrobial Agents
• 1. Inhibitors of Metabolism
• - Sulfonamides• - Trimethoprim
Classification of Antimicrobial Agents
• 2. Inhibitors of cell wall synthesis
• - B-lactams• - Vancomycin
Classification of Antimicrobial Agents• 3. Inhibitors of Protein
Synthesis• - Tetracycline• - Aminoglycosides• - Macrolides• - Clindamycin• - Chloramphenicol
• 4. Inhibitors of Nucleic Acid function or synthesis
• - Fluoroquinolones• - Rifampin
Classification of Antimicrobial Agents
Inhibitors of Cell wallSynthesisB-Lactams
Vancomycin
Inhibitors of metabolismSulfonamidesTrimethoprim
Inhibitors of ProteinSynthesis
TetracyclinesAminoglycosides
MacrolidesClindamycin
Chloramphenicol
InhibitorsOf Nucleic
Acid FunctionOr Synthesis
Fluoroquinolones
Rifampin
I. Inhibitors of Metabolism
• Folate Antagonists• Folic acid coenzyme are required for
the synthesis of purine and pyrimidine (RNA and DNA) and other compounds required for cellular growth and replication
• In the absence of folic acid cells cannot divide.
-
-
Amino acid biosynthesis
Purine synthesis
Pyrimidine synthesis
1. Sulfa drugs (SULFONAMIDES)
• Mechanism of action: • Inhibitors of folic acid synthesis, • - dye prontosil 1930’s
• Indications:• hemolytic streptococcal infections• low cost and efficacy in certain bacterial
infections UTI and trachoma• resistant strain, development of allergies and
the advent of Penicillin
Sulfa drugs (SULFONAMIDES)
• synergistic effect with Trimethoprim mid 1970’s (SULFAMETHOXAZOLE)
•
Sulfa drugs (SULFONAMIDES)
• Indications:– Pneumocystis carinii pneumonia or
Ampicillin-resistant or chloramphenicol resistant systemic salmonella infections
– Bacteriostatic– Enterobacteria, chlamydia, nocardia
Sulfa drugs (SULFONAMIDES)
• Pharmacokinetics
1. Absorption • Oral, Rectal, IV and Topical (Silver sulfadiazine)
2.Distribution • body water, CSF, cross the placenta, breast milk
3.Metabolism - Stone formation
4. Excretion • glomerular filtration
Adverse Effects:• 1. Crystalluria: Nephrotoxicity
• 2. HypersensitiVity – rashes and angioedema, Steven-Johnson syndrome
• 3. Hemopoietic disturbances – hemolytic anemia, G6PD
• 4. Kernicterus
• 5. Drug potentiation
Contraindications • A. Newborn• B. Infants less then 2 months• C. Pregnant women should not be
given in patients taking methenamine for UTI
2.Trimethoprim
• Mechanism of Action: • potent inhibitor of bacterial dihydrofolate reductase• compounded with sulfamethoxazole• 20 to 50 times more potent than the sulfonamides
• Indications• Acute UTI• Bacterial prostatitis
Adverse effects:• 1. folate defieciency• 2. megaloblastic anemia• 3. leukopenia and
granulocytopenia• Folinic acid can reversed the
deficiency
3. Co- trimoxazole
• Generic name Cotrimoxazole
• Brand name - Bactrim, Kathrex, Rimezone, Bacxal, Doctrimox, Triglobe, Triforam
• Mg/kg/day - 5 -8 mg/kg/day
• Preparations – 800mg/160mg/tab; 400mg/80mg/cap
400mg/80mg/5ml; 200mg/40mg/5ml
Co- trimoxazole• Trimethoprim plus sulfamethoxazole• Greater antimicrobial activity
• Mechanism of Action: inhibition of two sequential steps in the synthesis of tetrahydrofolic acid; sulfamethoxazole inhibits the incorporation of PABA into folic acid and trimethoprim prevents reduction of dihydrofolate to tetrahydrofolate
Co- trimoxazole• Ratio 20 parts of sulfamethoxazole
and 1 part trimethoprim• Orally• IV in Severe pneumonia caused by
Pneumocystitis carinii• Metabolites are excreted in the
urine
Co- trimoxazole• Adverse effects: • 1. Dermatologic• 2. Gastrointestinal: Nausea, vomiting,
glossitis and stomatitis• 3. Hematologic: Megaloblastic anemia,
leukopenia, thrombocytopenia• 4. HIV patients: PCP- drug-induced fever,
rashes and diarrhea and pancytopenia
Co- trimoxazole• Drug interactions:• 1. Prolonged Prothrombin time in-
patient receiving warfarin. • 2. Phenytoin may be increased due to
an inhibition of its metabolism. • 3. Methotrexate levels may rise due to
displacement of albumin binding sites
II. Inhibitors of Cell Wall Synthesis
• - B-lactams• - Vancomycin
Inhibitors of Cell wall Synthesis
B-lactam antibiotics Other antibiotics
Penicillins Cephalosporins Carbapenems Monobactams
Vancomycin
Bacitracin
ImipenemCilastin
Astreonam
Penicillins• Penicillin G• Penicillin V• Methicillin• Nafcillin• Oxacillin• Cloxacillin• Dicloxacillin
• Ampicillin• Amoxicillin• Carbenicillin• Ticarcillin• Piperacillin• Mezlocillin• Azlocillin
Cephalosporins• 1st Generation• Cefazolin• Cefadroxil• Cefalexin• Cefalothin• Cepharipin• Cefadrin
• 2nd Generation• Cefaclor• Cefamandole• Cefonizid• Cefmetazole• Cefotetan• Cefoxitin• Cefuroxime• Cefprozil• Loracarbef
Cephalosporins• 3rd generation• Cefixime• Cefoperazone• Cefotaxime• Ceftazidime• Ceftriaxone• Moxalactam• Cefdinir• Cefditoren pivoxil• Cefpodoxime• Ceftibuten• Ceftizoxime
• 4th generation• Cefepime (Maxipime)
B-Lactamase inhibitors• Clavulanic acid• Sulbactam• Tazobactam
Penicillin’s• – most widely effective
antibiotics and are among the least toxic drugs
• - major adverse reaction (Hypersensitivity)
• bactericidal
Penicillins• Penicillin G• Penicillin V• Methicillin• Nafcillin• Oxacillin• Cloxacillin• Dicloxacillin
• Ampicillin• Amoxicillin• Carbenicillin• Ticarcillin• Piperacillin• Mezlocillin• Azlocillin
Penicillin’s• Mechanism of Action:
– interfere with the last step of bacterial cell wall synthesis, exposing the osmotically less stable membrane
Mechanism of action/s:• rapidly growing organisms that
synthesize a peptidoglycan cell wall• Inactive against organisms devoid of
Peptidoglycan cell wall, such as mycobacteria, protozoa, fungi, viruses
• Inactivate proteins present on the bacterial cell membrane
Mechanism of action/s:• Penicillin binding protein • Enzymes for the synthesis of the cell wall
(morphology)• Methicillin-resistant Staphylococcus aureus• Inhibition of transpeptidase (cell wall integrity)• Autolysins – gram positive cocci• * Inhibition of cell synthesis and destruction of
existing cell wall by autolysins
Antibacterial Spectrum
• Gram positive• Gram negative
(lipopolysaccharide)
1. Natural Penicillin• – Penicillium chrysogenum
• Penicillin G (benzylpenicillin) – gram positive and gram negative cocci, gram positive bacilli and spirochetes
• Penicillin V - same spectrum with PenG, not used for treatment of bacteremia (MLC –minimum lethal concentration, mimimum amount of the drug needed to eliminate the infection), oral infections
2. Antistaphylococcal
penicillins:
• Methicillin, Nafcillin, oxacillin, cloxacillin and dicloxacillin- penicillinase-resistant penicillins
• Penicillinase-producing Staphylococci• Methicillin (toxic) (MRSA)
3. Extended spectrum penicillins
• Ampicillin and amoxicillin similar to PenG – gram-negative bacilli
• Ampicillin – gram-positive bacillus, Listeria monocytogenes
• Amoxicillin – dentists for abnormal heart valves
• Escherichia coli and Haemophilus influenzae (resistant
4. Antipseudomonal penicillins
• A. Carbenicillin• B. Ticarcillin• C. Piperacillin – most potent• Gram – negative bacilli but not
klebsiella
5. Penicillins and aminoglycosides
• – synergistic effect• -eg Ampicillin plus Gentamicin
Pharmacokinetics• 1. Administration• IV or IM - Methicillin, ticarcillin
carbenicillin, mezlocillin, piperacillin, azlocillin, combination of ampicillin with sulbactam, ticarcillin with clavulanic acid and piperacillin with tazobactam
• Oral - Pen V , amoxicillin and amoxicillin combined with clavulanic acid
Pharmacokinetics• 2. Absorption
– Penicillins are incompletely absorbed after oral medication and reach the intestine in sufficient amount to affect the intestinal flora. Amoxicillin is completely absorbed.
• - dec. by food and acidic environment • - 30-60 min before meals or 2 to 3
hours postprandially
Pharmacokinetics• 3. Distribution
– cross the placental barrier but none teratogenic
• - CSF insufficient
Pharmacokinetics• 4. Metabolism • 5. Excretion – kidney
Adverse reactions:
• A. Hypersensitivity• B. Diarrhea• C. Nephritis• D. Neurotoxicity – Seizure• E. Platelet dysfunction• F. Cation toxicity – Sodium – hypokalemia
Cephalosporins• 1st Generation• Cefazolin• Cefadroxil• Cefalexin• Cefalothin• Cepharipin• Cefadrin
• 2nd Generation• Cefaclor• Cefamandole• Cefonizid• Cefmetazole• Cefotetan• Cefoxitin• Cefuroxime• Cefprozil• Loracarbef
First generation:• Antibacterial Spectrum:• 1. PenG substitutes that are resistant to
Staphylococcal penicillinase
• Proteus mirabilis, Escherichia coli and klebsiella pneumoniae (PECK)
• e.g Cefazolin, Cefalexin*, Cephalothin, Cephapirin, Cephradine
Second generation:• Haemophilus influenzae, some
Enterobacter aerogenes and some Neisseria species (HENPECK)
• Gram-positive – weaker
• e.g Cefaclor, Cefamandine, Cefonizid, Cefmetazole, Cefotetan, Cefoxitin, Cefuroxime*
Third generation• – gram-positive cocci, gram-negative
bacilli
• Serratia marcencens• Pseudomonas aeruginosa
• E.g Cefixime, Cefoperazone, Cefotaxime, Ceftazidime, Ceftizoxime, Ceftriaxone
4th generation• Wide-coverage of microorganisms
Pharmacokinetics:
• Administration• Distribution• Metabolism• Elimination
Adverse effects:
• Allergic manifestations – 5 to 15 %. 1 to 2 %
• Disulfiram –like effect (Cefamandole) alcohol
• Bleeding – Cefamandole or Cefoperazone (antivita. K)
Other B-lactam Antibiotics
• 1. Carbapenems – Imipenem/Cilastatin• gram positive, gram negative, aerobes and
Pseudomonas aeruginosa
• Pharmacokinetics: Imipenem – IV CSF, GFR, Nephrotoxic
• Adverse effects:• Nausea• Vomiting• Diarrhea• Seizure
Monobactams• 1. Aztreonam
• Enterobacteria• Aerobic gram negative rods• Lacks activity against gram positive and anaerobes• IV and IM• Urine• Adverse effects:
• Phlebitis• skin rash
Relatively nontoxic a safe alternative fro treating patients allergic to penicillins and cephalosporins
B- Lactamase Inhibitors
• A. Clavulanic acid• B. Sulbactam• C. Tazobactam
Other Agents Affecting the Cell
Wall
• 1. Vancomycin • Mode of Action:
– Inhibits synthesis of bacterial cell wall phospholipids as well as peptidoglycan polymerization
• Clostridium difficile or staphylococci• Prophylactic treatment among dental patients• Prosthetic heart valves• Prosthetic devices• Aminoglycosides for enterococcal endocarditis
Pharmacokinetics:•• Slow intravenous infusion• Not absorbed after oral
administration• Metabolism is minimal
Adverse effects: • Fever• Chills• Phlebitis• Shock• Flushing (red man syndrome)
Bacitracin
• Gram- positive organisms• Topical application• Nephrotoxicity