priloga b k pk vf, datum: 20.12.2018 1/78 seznam metod s ... · priloga b k pk vf, datum:...

Priloga B k PK VF, datum: 20.12.2018 1/78 Attachment B to PK VF, date: 20.12. 2018 Seznam metod s podano merilno negotovostjo/mejami metode The list of methods with measurement uncertainty/method limits

* Trenutno veljavni obseg akreditacije je dostopen na spletni strani SA (http://www.slo-akreditacija.si/acreditation/univerza-v-ljubljani-veterinarska-fakulteta/) Z rumeno so označene spremembe od predhodne verzije priloge B.

Inštitut za varno hrano, krmo in okolje; Enota za varno hrano Št. No.

Oznaka preskusne metode Internal/standard reference

number

Preskusna metoda Test method

Materiali, proizvodi; Merilna negotovost (podana kot razširjena merilna negotovost U); Meje metode

Material, products; Measurement uncertainty (expressed as expanded measurement uncertainty U); Method limits

Akreditirano* Da/Ne

Accredited Yes/No

1. SOP 173, verzija 2 interna metoda/in-house method

Določanje histamina v ribah in ribjih izdelkih s HPTLC– presejalna metoda Determining histamine in fish and fish products with HPTLC – screening method

ribe in ribji proizvodi (razen proizvodi v slanici) / fish and fish products (except products in salt water) (50–200 mg/kg) LOQ = 50 mg/kg ±28 mg/kg

DA / YES

2. SOP 305, verzija 4 interna metoda in-house method

Določanje ostankov skupnih metabolitov nitrofuranov z LC-MS/MS, Determining residues of total nitrofurans metabolites with LC-MS/MS

meso / meat (0,5–5,0) μg/kg 3-amino-2-oxolidinone (AOZ) CC = 0,63 μg/kg CC = 0,76 μg/kg ±0,37 μg/kg (1 μg/kg) ±0,65 μg/kg (1,5 μg/kg) ±0,43 μg/kg (2 μg/kg) 5-methylmorfolino-3-amino-2-oxazolidinone (AMOZ) CC = 0,57 μg/kg CC = 0,63 μg/kg ±0,64 μg/kg (1 μg/kg) ±0,50 μg/kg (1,5 μg/kg) ±1,00 μg/kg (2 μg/kg) 1-amino-hydantoine hydrochloride (AHD) CC = 0,58 μg/kg CC = 0,66 μg/kg ±0,38 μg/kg (1 μg/kg) ±0,77 μg/kg (1,5 μg/kg) ±0,59 μg/kg (2 μg/kg) semicarbazide hydrochloride (SEM) CC = 0,59 μg/kg CC = 0,68 μg/kg ±0,61 μg/kg (1 μg/kg) ±0,46 μg/kg (1,5 μg/kg) ±0,55 μg/kg (2 μg/kg) jajca / eggs (0,5–5,0) μg/kg 3-amino-2-oxolidinone (AOZ) CC = 0,54μg/kg CCβ = 0,59 μg/kg ±0,10 μg/kg (0,5 μg/kg) ±0,10 μg/kg (1,0 μg/kg) ±0,19 μg/kg (1,5 μg/kg) 5-methylmorfolino-3-amino-2-oxazolidinone (AMOZ) CC = 0,57 μg/kg CCβ = 0,64 μg/kg ±0,15 μg/kg (0,5 μg/kg) ±0,25 μg/kg (1,0 μg/kg) ±0,34 μg/kg (1,5 μg/kg) 1-amino-hydantoine hydrochloride (AHD) CC = 0,57 μg/kg CCβ = 0,63 μg/kg ±0,16 μg/kg (0,5 μg/kg) ±0,13 μg/kg (1,0 μg/kg) ±0,30 μg/kg (1,5 μg/kg) semicarbazide hydrochloride (SEM) CC = 0,50 μg/kg CCβ = 0,51 μg/kg ±0,004 μg/kg (0,5 μg/kg) ±0,02 μg/kg (1,0 μg/kg) ±0,03 μg/kg (1,5 μg /kg)

DA / YES DA / YES

3. SOP 372, verzija 3

Določanje domoične kisline v školjkah s HPLC z UV detektorjem Determination of Domoic acid in mussles and finfish by HPLC with UV detection

školjke / shellfish užitna klapavica (Mytillus galloprovincialis) domoična kislina / domoic acid (3–75) μg/kg LOQ = 3 μg/kg

DA / YES


Določanje vsebnosti klorida (kot NaCl) v mesnih izdelkih – Mohrova metoda Determination of chloride (as NaCl) content in meat products – Mohr method

mesni izdelki / meat products (1 – 7) g/100g ± 9 % za vsebnost NaCl ≤ 1,5 g/100 g ± 5 % za vsebnost NaCl > 1,5 g/100 g

DA / YES





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Akreditirano* Da/Ne

Accredited Yes/No

5. ISO 1871: 2009 Določanje dušika in beljakovin po Kjeldahlu Preračun za % beljakovin: faktor 6,25

Determination of nitrogenand proteins by the Kjeldahl method Calculation to % of protein: factor 6,25 SOP 424, verzija 2

meso in mesni izdelki / meat and meat products N: (1 – 6,5) g/100g ± 2,5 % sušene mesnine / dry meat proucts ± 3,0 % mleko / milk N: (0,4–1) g/100g ±2,5 %

DA / YES DA / YES DA / YES

6. AOAC Official Method 2007.04: 2007

Postopek za določanje maščobe, vode in beljakovin z NIR spektrometrijo Procedure for determination of fat, moisture and protein in meat and meat products SOP 484, verzija 1

meso in mesni izdelki/ meat and meat products Beljakovine/protein 9 – 30 g/100 g ± 6,7 % Maščobe/fat: 6 – 40 g/100 g ± 10 % 1 – 5,9 g/100 g ± 28 % Voda/moisture: 30 – 80 g/100 g ± 4,5 %

DA / YES


Ugotavljanje ostankov makrocikličnih laktonov s HPLC-fluorescenco Determining macrocyclic lactone residues by HPLC-fluorescence

mleko / milk ivermektin / ivermectin (IVM) (0,1–60) μg H2B1a/kg CCα = 0,1 μg H2B1a/kg CCβ = 0,14 μg H2B1a/kg ±0,27 μg H2B1a/kg (2 μgH2B1a/kg) doramektin / doramectin (DOR) (0,1–60) μg/kg CCα = 0,1 μg/kg CCβ = 0,14 μg/kg ±0,35 μg/kg (2 μg/kg) abamektin / abamectin (ABM) (0,1–60) μg B1a /kg CCα = 0,1 μg/kg CCβ = 0,14 μg B1a /kg ±0,35 μg B1a /kg (2 μg B1a /kg) eprinomektin / eprinomectin (EPR) (0,2–60) μg EPR B1a/kg CCα = 24,8 μg EPR B1a/kg CCβ = 27,8 μg EPR B1a/kg ±3,7 μg EPR B1a/kg (20 μg EPR B1a/kg) LOD = 0,2 μg EPR B1a/kg moksidektin / moxidectin (MOX) (0,2–60) μg/kg CCα = 50,6 μg/kg CCβ = 55,2 μg/kg ±6,2 μg/kg (40 μg/kg) LOD = 0,2 μg/kg emamektin / eprinomectin (EMA) (0,2–60) μg EMA B1a/kg CCα = 0,2 μg EMA B1a/kg CCβ = 0,27 μg EMA B1a/kg ±0,6 μg EMA B1a/kg (2 μg EMA B1a/kg) nemadektin / nemadectin (NEM) (0,2–60) μg/kg CCα = 0,2 μg/kg CCβ = 0,25 μg/kg ±0,3 μg/kg (2 μg/kg) maščoba perutnine / poultry fat ivermektin / ivermectin (IVM) (1–100) μg H2B1a/kg CCα = 1,1 μg/kg CCβ = 1,2 μg/kg

DA / YES DA / YES





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Akreditirano* Da/Ne

Accredited Yes/No

doramectin / doramectin (DOR) (1–100) μg/kg CCα = 1,1 μg/kg CCβ = 1,3 μg/kg abamectin / abamectin (ABM) (1–100) μg B1a /kg CCα = 1,1 μg/kg CCβ = 1,4 μg/kg eprinomektin / eprinomectin (EPR) (1–100) μg EPR B1a/kg CCα = 1,2 μg/kg CCβ = 1,3 μg/kg moksidektin / moxidectin (MOX) (1–100) μg/kg CCα = 1,1 μg/kg CCβ = 1,2 μg/kg emamektin / emamectin (EMA) (1–100) μg EMA B1a/kg CCα = 1,2 μg/kg CCβ = 1,5 μg/kg nemadektin / nemadectin (1–100) μg/kg CCα = 1,1 μg/kg CCβ = 1,2 μg/kg prašičja maščoba / porcine fat ivermektin / ivermectin (IVM) (1–200) μg H2B1a/kg CCα = 108 μg/kg CCβ = 118 μg/kg doramectin / doramectin (DOR) (1–250) μg/kg CCα = 183 μg/kg CCβ = 204 μg/kg abamectin / abamectin (ABM) (1–100) μg B1a /kg CCα = 1,1 μg/kg CCβ = 1,3 μg/kg eprinomektin / eprinomectin (EPR) (1–100) μg EPR B1a/kg CCα = 1,2 μg/kg CCβ = 1,4 μg/kg moksidektin / moxidectin (MOX) (1–100) μg/kg CCα = 1,1 μg/kg CCβ = 1,2 μg/kg emamektin /emamectin (EMA) (1–100) μg EMA B1a/kg CCα = 1,2 μg/kg CCβ = 1,5 μg/kg nemadektin / nemadectin (NEM) (1–100) μg/kg CCα = 1,2 μg/kg CCβ = 1,3 μg/kg goveja maščoba / bovine fat ivermektin / ivermectin (IVM) (1–200) μg H2B1a/kg CCα = 107 μg/kg CCβ = 114 μg/kg doramectin / doramectin (DOR) (1–250) μg/kg CCα = 166 μg/kg CCβ = 179 μg/kg abamectin / abamectin (ABM) (1–100) μg B1a /kg CCα = 12 μg/kg CCβ = 14 μg/kg

DA / YES DA / YES





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Akreditirano* Da/Ne

Accredited Yes/No

eprinomektin / eprinomectin (EPR) (1–400) μg EPR B1a/kg CCα = 276 μg/kg CCβ = 294 μg/kg moksidektin / moxidectin (MOX) (1–800) μg/kg CCα = 531 μg/kg CCβ = 572 μg/kg emamektin / emamectin (EMA) (1–100) μg EMA B1a/kg CCα = 1,2 μg/kg CCβ = 1,5 μg/kg nemadektin / nemadectin (NEM) (1–100) μg/kg CCα = 1,2 μg/kg CCβ = 1,4 μg/kg


Določanje ostankov sulfonamidov s HPLC fluorescenčno detekcijo Determining sulfonamide residues by HPLC-fluorescence

meso / meat (25–250) μg/kg sulfatiazol / sulfathiazole (STZ), ±16 μg/kg (100 μg/kg) CCα = 113 μg/kg CCβ = 126 μg/kg sulfadiazin / sulfadiazine (SDZ), ±17 μg/kg (100 μg/kg) CCα = 112 μg/kg CCβ = 124 μg/kg sulfamerazin / sulfamerazine (SRZ), ±15 μg/kg (100 μg/kg) CCα = 112 μg/kg CCβ = 124 μg/kg sulfametazin / sulfamethazine (SMZ), ±14 μg/kg (100 μg/kg) CCα = 111 μg/kg CCβ = 123 μg/kg sulfamoksol / sulfamoxole (SMO), ±20 μg/kg (100 μg/kg) CCα = 116 μg/kg CCβ = 133 μg/kg sulfamonometoksin / sulfamonomethoxine (SMM), ±15 μg/kg (100 μg/kg) CCα = 113 μg/kg CCβ = 125 μg/kg sulfametizol / sulfamethizole (SMTZ), ±38 μg/kg (100 μg/kg) CCα = 112 μg/kg CCβ = 124 μg/kg sulfakloropiridazin / sulfachloropyridazine (SCP), ±17 μg/kg (100 μg/kg) CCα = 114 μg/kg CCβ = 128 μg/kg sulfadoksin / sulfadoxine (SDX), ±16 μg/kg (100 μg/kg) CCα = 113 μg/kg CCβ = 127 μg/kg sulfametoksazol / sulfamethoxazole (SMX), ±17 μg/kg (100 μg/kg) CCα = 114 μg/kg CCβ = 129 μg/kg sulfisoksazol / sulfisoxasole (SFX), ±20 μg/kg (100 μg/kg) CCα = 115 μg/kg CCβ = 131 μg/kg sulfadimetoksin / sulfadimethoxine (SDM), ±16 μg/kg (100 μg/kg) CCα = 113 μg/kg CCβ = 126 μg/kg

DA / YES





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Akreditirano* Da/Ne

Accredited Yes/No

sulfakinoksolin / sulfaquinoxaline (SQX), ±22 μg/kg (100 μg/kg) CCα = 118 μg/kg CCβ = 137 μg/kg sulfafenazol / sulfaphenazole (SPZ) ±18 μg/kg (100 μg/kg) CCα = 114 μg/kg CCβ = 129 μg/kg jajca / eggs presejalna metoda / screening method CCβ = 20 μg/kg sulfisoksazol / sulfisoxasole (SFX) CCβ = 10 μg/kg sulfamoksol / sulfamoxole (SMO) sulfakloropiridazin / sulfachloropyridazine (SCP) CCβ = 5 μg/kg sulfatiazol / sulfathiazole (STZ) sulfadiazin / sulfadiazine (SDZ) sulfamerazin / sulfamerazine (SRZ) sulfametazin / sulfamethazine (SMZ) sulfamonometoksin / sulfamonomethoxine (SMM) sulfadoksin / sulfadoxine (SDX) sulfametoksazol / sulfamethoxazole (SMX) sulfadimetoksin / sulfadimethoxine (SDM) sulfakinoksalin / sulfaquinoxaline (SQX) sulfafenazol / sulfaphenazole (SPZ) sulfapiridin / sulfapyridine (SPY) sulfanilamid / sulfanilamide (SAD) sulfagvanidin / sulfaguanidine (SG)

DA / YES


Ugotavljanje prisotnosti sulfonamidov HPTLC - presejalna metoda Determining of sulfonamides HPTLC - screening method

meso / meat sulfagvanidin / sulfaguanidine (SG) sulfanilamid / sulfanilamide (SAD) sulfatiazol / sulfathiazole (STZ) sulfapiridin / sulfapyridine (SPY) sulfadiazin / sulfadiazine (SDZ) sulfamerazin / sulfamerazine (SRZ) sulfametazin / sulfamethazine (SMZ) sulfamonometoksin / sulfamonomethoxine (SMM) sulfametizol / sulfamethizole (SMTZ) sulfakloropiridazin / sulfachloropyridazine (SCP) sulfametoksazol / sulfamethoxazole (SMX) sulfisoksazol / sulfisoxasole (SFX) sulfadimetoksin / sulfadimethoxine (SDM) sulfakvinoksalin // sulfaquinoxaline (SQX) sulfadoksin / sulfadoxine (SDX) sulfafenazol / sulfaphenazole (SPZ) sulfamoksol / sulfamoxole (SMO) sulfametoksipiridazin / sulfachloropyridazine (SMP) CCβ = 25 μg/kg

DA / YES


Določanje PSP toksinov v školjkah s HPLC-FLD – presejalna metoda Determination of PSP toxins in shellfish with HPLC-FLD – screening method

školjke / mussels užitna klapavica (Mytilus galloprovincialis) CCβ = 300 μg/kg neosaxitoxin (NEO) gonyautoxin 1 in / and gonyautoxin 4 (GTX1,4) CCβ = 100 μg/kg saxitoxin (STX) decarbamoylsaxitoxin (dc STX) gonyautoxin 5 (GTX 5) gonyautoxin 2 in / and gonyautoxin 3 (GTX2,3) decarbamoylgonyautoxin 2 in / and decarbamoylgonyautoxin 3 (dc GTX2,3) N-sulfocarbamoyl-gonyautoxin 1 in / and N-sulfocarbamoyl-gonyautoxin 2 (C1,2)

DA / YES


Določanje lipofilnih toksinov v školjkah s LC-MS/MS - presejalna metoda Determination of lipophilic toxins in shellfish with LC-MS/MS – screening method Potrditvena metoda / Confirmatory method

školjke / mussels užitna klapavica (Mytillus galloprovincialis) Presejalna metoda/ screening method CCβ = 32 μg/kg okadaic acid group (OA, DTX1, DTX2, DTX3) pectenotoxins group (PTX1, PTX2) azaspiracids group (AZA1, AZA2, AZA3) SPX1 GYM

DA / YES DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

CCβ = 200 μg/kg yessotoxins group (YTX, 45 OH YTX, homo YTX, 45 OH homo YTX) Potrditvena metoda /Confirmatory method okadaic acid group(OA) (32–320) μg/kg CCα = 173 μg/kg CCβ = 186 μg/kg ±8.6 μg/kg (80 μg/kg) ±17 μg/kg (160 μg/kg) ±25 μg/kg (240 μg/kg) pectenotoxins group (PTX) (32–320) μg/kg CCα = 187 μg/kg CCβ = 214 μg/kg ±15 μg/kg (80 μg/kg) ±36 μg/kg (160 μg/kg) ±42 μg/kg (240 μg/kg) azaspiracids group (AZA) (32–320) μg/kg CCα = 182 μg/kg CCβ = 203 μg/kg ±11 μg/kg (80 μg/kg) ±26 μg/kg (160 μg/kg) ±50 μg/kg (240 μg/kg) SPX1 (32–320) μg/kg CCα = 179 μg/kg CCβ = 199 μg/kg ±17 μg/kg (80 μg/kg) ±41 μg/kg (160 μg/kg) ±43 μg/kg (240 μg/kg) GYM (32–320) μg/kg CCα = 182 μg/kg CCβ = 204 μg/kg ±7.9 μg/kg (80 μg/kg) ±27 μg/kg (160 μg/kg) ±32 μg/kg (240 μg/kg) yessotoxins group (YTX) (200–2000) μg/kg CCα = 4450 μg/kg CCβ = 5150 μg/kg ±99 μg/kg (500 μg/kg) ±186 μg/kg (1000 μg/kg) ±341 μg/kg (2000 μg/kg)

DA / YES


Določanje ostankov nitroimidazolov in pripadajočih metabolitov s HPLC-DAD Determining nitroimidazoles and methabolites with HPLC-DAD

plazma / plasma: (2 – 12) ng/mL dimetridazol / dimetridazole (DMZ): CCα = 2 ng/ml CCβ = 2,5 ng/ml ±1,2 ng/ml (5 ng/ml) ronidazol / ronidazole (RNZ): CCα = 2 ng/ml CCβ = 2,4 ng/ml ±1,3 ng/ml (5 ng/ml) hidroksidimetridazol / hydroxymetridazole (DMZ-OH): CCα = 2 ng/ml CCβ = 2,3 ng/ml ±1,6 ng/ml (5 ng/ml) metronidazol / metronidazole (MNZ): CCα = 2 ng/ml CCβ = 2,3 ng/ml ±1,3 ng/ml (5 ng/ml) hidroksimetronidazol / hydroxymetronidazole (MNZ-OH): CCα = 2 ng/ml CCβ = 2,2 ng/ml ±0,8 ng/ml (5 ng/ml)

DA / YES Da / yes Da / yes Da / yes Da / yes Da / yes





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Akreditirano* Da/Ne

Accredited Yes/No

seknidazol / secnidazole (SNZ) CCα = 2 ng/ml tinidatzol / tinidazole (TNZ) CCα = 2 ng/ml hidroksiipronidazol / hydroxyiprondazole (IPZOH) CCα = 2 ng/ml ornidazol / ornidazole (ONZ) CCα = 2 ng/ml mleko / milk dimetridazol / dimetridazole (DMZ) (0,5–12) µg/kg CCα = 0,5 µg/kg CCβ = 0,6 µg/kg (1–12) µg/kg ronidazol / ronidazole (RNZ) CCα = 1 µg/kg CCβ = 1,3 µg/kg hidroksidimetridazol / hydroxymetridazole (DMZ-OH) CCα = 1 µg/kg CCβ = 1,2 µg/kg metronidazol / metronidazole (MNZ) CCα = 1 µg/kg CCβ = 1,3 µg/kg hidroksimetronidazol / hydroxymetronidazole (MNZ-OH) CCα = 1 µg/kg CCβ = 1,2 µg/kg seknidazol / secnidazole (SNZ) CCα = 1 µg/kg tinidatzol / tinidazole (TNZ) CCα = 1 µg/kg hidroksiipronidazol / hydroxyiprondazole (IPZOH) CCα = 1 µg/kg ornidazol / ornidazole (ONZ) CCα = 1 µg/kg jajca / eggs dimetridazol / dimetridazole (DMZ) (0,5–12) µg/kg CCα = 0,5 µg/kg CCβ = 1,0 µg/kg (1–12) µg/kg ronidazol / ronidazole (RNZ) CCα = 1 µg/kg CCβ = 1,4 µg/kg hidroksidimetridazol / hydroxymetridazole (DMZ-OH) CCα = 1 µg/kg CCβ = 1,1 µg/kg metronidazol / metronidazole (MNZ) CCα = 1 µg/kg CCβ = 1,2 µg/kg hidroksimetronidazol / hydroxymetronidazole (MNZ-OH) CCα = 1 µg/kg CCβ = 1,2 µg/kg seknidazol / secnidazole (SNZ) CCα = 1 µg/kg tinidatzol / tinidazole (TNZ) CCα = 1 µg/kg hidroksiipronidazol / hydroxyiprondazole (IPZOH) CCα = 1 µg/kg ornidazol / ornidazole (ONZ) CCα = 1 µg/kg

Ne / No Ne / No Ne / No Ne / No DA / YES Da / yes Da / yes Da / yes Da / yes Da / yes Ne / No Ne / No Ne / No Ne / No DA / YES Da / yes Da / yes Da / yes Da / yes Da / yes Ne / No Ne / No Ne / No Ne / No


Ugotavljanje ostankov kinolonov s HPLC-fluorescenco Determining quinolone residues by HPLC-fluorescence

meso / meat norfloksacin / norfloxacin (NOR) (20–60) µg/kg LOQ = 20 μg/kg ±2,5 μg/kg (20 μg/kg)

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

±3,2 μg/kg (40 μg/kg) ±5,9 μg/kg (60 μg/kg) ofloksacin / orbifloxacin (OFC) (30–90) µg/kg LOQ = 30 μg/kg ±5,8 μg/kg (30 μg/kg) ±10,0 μg/kg (60 μg/kg) ±19,4 μg/kg (90 μg/kg) ciprofloksacin / ciprofloxacin (CPF) (20–150) µg/kg ±3,6 μg/kg (50 μg/kg) ±5,9 μg/kg (100 μg/kg) ±13,0 μg/kg (150 μg/kg) perutnina, svinjina, zajci / poultry, porcine, rabbit: CCα = 105 µg/kg CCβ = 110 µg/kg govedina, ovčetina, koze / bovine, ovine, caprine: CCα = 104 µg/kg CCβ = 109 µg/kg ribe / fish: CCα = 106 µg/kg CCβ = 112 µg/kg enrofloksacin / enrofloxacin (EFC) (5–150) µg/kg ±7,9 μg/kg (50 μg/kg) ±12,0 μg/kg (100 μg/kg) ±24,6 μg/kg (150 μg/kg) perutnina, ovčetina, koze / poultry, ovine, caprine: CCα = 110 µg/kg CCβ = 121 µg/kg govedina, zajci / bovine, rabbit: CCα = 106 µg/kg CCβ = 111 µg/kg svinjina / porcine: CCα = 107 µg/kg CCβ = 115 µg/kg ribe / fish: CCα = 108 µg/kg CCβ = 116 µg/kg sarafloksacin / sarafloxacin (SAR) (10–30) µg/kg LOQ = 10 μg /kg ±1,1 μg/kg (10 μg/kg) ±1,3 μg/kg (20 μg/kg) ±2,8 μg/kg (30 μg/kg) difloksacin / difloxacin (DIF) (5–450) µg/kg ±35,1 μg/kg (150 μg/kg) ±68,4 μg/kg (300 μg/kg) ±124,0 μg/kg (450 μg/kg) perutnina / poultry: CCα = 358 µg/kg CCβ = 416 µg/kg ovčetina in koze / ovine and caprine: CCα = 463 µg/kg CCβ = 526 µg/kg govedina / bovine: CCα = 423 µg/kg CCβ = 446 µg/kg zajci / rabbit: CCα = 326 µg/kg CCβ = 352 µg/kg svinjina / porcine: CCα = 436 µg/kg CCβ = 473 µg/kg ribe / fish: CCα = 327 µg/kg CCβ = 354 µg/kg oksolinska kislina / oxolinic acid (OXO) (20–150) µg/kg ±8,8 μg/kg (50 μg/kg) ±14,5 μg/kg (100 μg/kg) ±31,3 μg/kg (150 μg/kg) perutnina / poultry: CCα = 112 µg/kg CCβ = 125 µg/kg svinjina, ovčetina in koze / porcine, ovine and





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Akreditirano* Da/Ne

Accredited Yes/No

caprine: CCα = 105 µg/kg CCβ = 110 µg/kg govedina in zajci / bovine and rabbit: CCα = 104 µg/kg CCβ = 109 µg/kg ribe / fish: CCα = 105 µg/kg CCβ = 110 µg/kg nalidiksična kislina / nalidixic acid (NAL) (20–60) µg/kg LOQ = 20 μg /kg ±5,1 μg/kg (20 μg/kg) ±8,8 μg/kg (40 μg/kg) ±18,6 μg/kg (60 μg/kg) flumekvin / flumequin (FLU) (10–600) µg/kg ±53,7 μg/kg (200 μg/kg) ±120,8 μg/kg (400 μg/kg) ±220,3 μg/kg (600 μg/kg) perutnina / poultry: CCα = 501 µg/kg CCβ = 616 µg/kg ovčetina in koze / ovine and caprine: CCα = 241 µg/kg CCβ = 281 µg/kg govedina / bovine: CCα = 220 µg/kg CCβ = 240 µg/kg zajci / rabbit: CCα = 227 µg/kg CCβ = 255 µg/kg svinjina / porcine: CCα = 229 µg/kg CCβ = 258 µg/kg ribe / fish: CCα = 686 µg/kg CCβ = 772 µg/kg marbofloksacin / marbofloxacin (MAR) (20–60) µg/kg ±2,1 μg/kg (20 μg/kg) ±3,1 μg/kg (40 μg/kg) ±5,8 μg/kg (60 μg/kg) govedina / bovine: CCα = 158 µg/kg CCβ = 165 µg/kg Svinjina: CCα = 160 µg/kg CCβ = 170 µg/kg danofloksacin / danofloxacin (DAN) (5–300) µg/kg ±7,8 μg/kg (100 μg/kg) ±12,2 μg/kg (200 μg/kg) ±27,9 μg/kg (300 μg/kg) perutnina, govedina, ovčetina in koze / poultry, bovine, ovine and caprine: CCα = 212 µg/kg CCβ = 224 µg/kg svinjina in zajci / porcine and rabbit: CCα = 107 µg/kg CCβ = 115 µg/kg ribe / fish: CCα = 109 µg/kg CCβ = 118 µg/kg orbifloksacin / orbifloxacin (ORB) 10–30 µg/kg LOQ = 10 μg /kg ±2,6 μg/kg (10 μg/kg) ±4,2 μg/kg (20 μg/kg) ±6,9 μg/kg (30 μg/kg) jajca / eggs norfloksacin / norfloxacin (NOR) (20–1000) µg/kg LOQ = 20 μg /kg CCα = 20,7 µg/kg CCβ = 21,4 µg/kg ±1,0 μg/kg (20 μg/kg)

DA / YES





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Akreditirano* Da/Ne

Accredited Yes/No

±1,9 μg/kg (40 μg/kg) ±2,5 μg/kg (60 μg/kg) ofloksacin / ofloxacin (OFC) (30–1000) µg/kg LOQ = 30 μg /kg CCα = 33,3 µg/kg CCβ = 36,5 µg/kg ±5,9 μg/kg (30 μg/kg) ±5,2 μg/kg (60 μg/kg) ±5,8 μg/kg (90 μg/kg) ciprofloksacin / ciprofloxacin (CPF) (10–1000) µg/kg LOQ = 10 μg /kg CCα = 10,8 µg/kg CCβ = 11,5 µg/kg ±1,4 μg/kg (10 μg/kg) ±1,9 μg/kg (20 μg/kg) ±1,4 μg/kg (30 μg/kg) enrofloksacin / enrofloxacin (EFC) (5–1000) µg/kg LOQ = 5 μg /kg CCα = 6,03 µg/kg CCβ = 7,05 µg/kg ±1,2 μg/kg (5 μg/kg) ±1,2 μg/kg (10 μg/kg) ±1,4 μg/kg (15 μg/kg) sarafloksacin / sarafloxacin (SAR) (10–1000) µg/kg LOQ = 10 μg /kg CCα = 10,2 µg/kg CCβ = 10,5 µg/kg ±0,31 μg/kg (10 μg/kg) ±0,97 μg/kg (20 μg/kg) ±1,6 μg/kg (30 μg/kg) difloksacin / difloxacin (DIF) (5–1000) µg/kg LOQ = 5 μg /kg CCα = 5,35 µg/kg CCβ = 5,69 µg/kg ±0,28 μg/kg (5 μg/kg) ±0,62 μg/kg (10 μg/kg) ±0,92 μg/kg (15 μg/kg) oksolinska kislina / oxolinic acid (OXO) (20–1000) µg/kg LOQ = 20 μg /kg CCα = 21,1 µg/kg CCβ = 22,3 µg/kg ±0,87 μg/kg (20 μg/kg) ±1,7 μg/kg (40 μg/kg) ±3,4 μg/kg (60 μg/kg) nalidiksična kislina / nalidixic acid (NAL) (20–1000) µg/kg LOQ = 20 μg /kg CCα = 21,3 µg/kg CCβ = 22,7 µg/kg ±1,4 μg/kg (20 μg/kg) ±2,1 μg/kg (40 μg/kg) ±3,8 μg/kg (60 μg/kg) flumekvin / flumequin (FLU) (10–1000) µg/kg LOQ = 10 μg /kg CCα = 11,7 µg/kg CCβ = 13,3 µg/kg ±2,5 μg/kg (10 μg/kg) ±1,3 μg/kg (20 μg/kg) ±1,7 μg/kg (30 μg/kg) marbofloksacin / marbofloxacin (MAR) (20–1000) µg/kg LOQ = 20 μg /kg CCα = 21,7 µg/kg CCβ = 23,3 µg/kg ±2,8 μg/kg (20 μg/kg) ±4,2 μg/kg (40 μg/kg) ±6,2 μg/kg (60 μg/kg)





number




Akreditirano* Da/Ne

Accredited Yes/No

danofloksacin / danofloxacin (DAN) (5–300) µg/kg LOQ = 5 μg /kg CCα = 5,23 µg/kg CCβ = 5,47 µg/kg ±0,39 μg/kg (5 μg/kg) ±0,67 μg/kg (10 μg/kg) ±0,95 μg/kg (15 μg/kg) orbifloksacin / orbifloxacin (ORB) (10–1000) µg/kg LOQ = 10 μg /kg CCα = 10,5 µg/kg CCβ = 11,0 µg/kg ±0,54 μg/kg (10 μg/kg) ±0,98 μg/kg (20 μg/kg) ±1,4 μg/kg (30 μg/kg)


Določanje ostankov NSAID z LC-MS/MS Determining residues of NSAID with LC-MS/MS

mleko / milk: karprofen/carprofen (CPF): (50–1000) µg/kg CCα = 64,7 μg/kg CCβ = 84,1 μg/kg ±23,1 μg/kg (50 μg/kg) ±31 μg/kg (100 μg/kg) ±41 μg/kg (150 μg/kg) ketoprofen / ketoprofen (KPF): (2–1000) µg/kg CCα = 2,49 μg/kg CCβ = 2,97μg/kg ±0,50 μg/kg (1 μg/kg) ±0,66 μg/kg (2 μg/kg) ±0,90 μg/kg (3 μg/kg) fluniksin / flunixin (FLU): (1–1000) µg/kg CCα = 45,4 μg/kg CCβ = 51,9 μg/kg ±2,3 μg/kg (20 μg/kg) ±3,6 μg/kg (40 μg/kg) ±9,3 μg/kg (60 μg/kg) tolfenamska kislina / tolfenamic acid (TFA): (5–1000) µg/kg CCα = 55,7 μg/kg CCβ = 62,6 μg/kg ±4,5 μg/kg (25 μg/kg) ±8,1 μg/kg (50 μg/kg) ±13,1 μg/kg (75 μg/kg) meloksikam / meloxicam (MLX): (1–1000) µg/kg CCα = 16,5 μg/kg CCβ = 17,9 μg/kg ±1,38 μg/kg (7,5 μg/kg) ±1,7 μg/kg (15 μg/kg) ±3,2 μg/kg (23 μg/kg) 5-hidroksifluniksin / 5-hydroxyflunixin (FLU-OH): (1–1000) µg/kg CCα = 47,1 μg/kg CCβ = 50,7 μg/kg ±4,1 μg/kg (20 μg/kg) ±8,2 μg/kg (40 μg/kg) ±12,6 μg/kg (60 μg/kg) oksifenilbutazon / oxyphenbutazon (OBZ): (5–1000) µg/kg CCα = 5,89 μg/kg CCβ = 6,89 μg/kg ±3,74 μg/kg (5 μg/kg) ±3,8 μg/kg (10 μg/kg) ±2,9 μg/kg (15 μg/kg) diklofenak / diclofenac (DCL): (2–1000) µg/kg CCα = 3,03 μg/kg CCβ = 3,65 μg/kg ±1,12 μg/kg (1 μg/kg) ±1,08 μg/kg (2 μg/kg) ±1,16 μg/kg (3 μg/kg)

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

fenilbutazon / phenylbutazone (PBZ): (5–1000) µg/kg CCα = 6,52 μg/kg CCβ = 8,31 μg/kg ±1,70 μg/kg (5 μg/kg) ±2,4 μg/kg (10 μg/kg) ±2,7 μg/kg (15 μg/kg) plazma / plasma karprofen / carprofen CPF): (50–1000) µg/kg CCα = 62 μg/kg CCβ = 79 μg/kg ±7,1 μg/kg (50 μg/kg) ±6,9 μg/kg (100 μg/kg) ±6,2 μg/kg (150 μg/kg) ketoprofen / ketoprofen (KPF): (10–100) µg/kg CCα = 11 μg/kg CCβ = 12 μg/kg ±1,7 μg/kg (10 μg/kg) ±3,0 μg/kg (20 μg/kg) ±3,6 μg/kg (30 μg/kg) fluniksin / flunixin (FLU): (1–20) µg/kg CCα = 1,1 μg/kg CCβ = 1,3 μg/kg ±0,16 μg/kg (1 μg/kg) ±0,31 μg/kg (2 μg/kg) ±0,38 μg/kg (3 μg/kg) tolfenamska kislina / tolfenamic acid (TFA): (20–200) µg/kg CCα = 23 μg/kg CCβ = 24 μg/kg ±1,6 μg/kg (20 μg/kg) ±1,7 μg/kg (40 μg/kg) ±4,2 μg/kg (60 μg/kg) meloksikam / meloxicam (MLX): (5–100) µg/kg CCα = 5,6 μg/kg CCβ = 6,2 μg/kg ±1,0 μg/kg (5 μg/kg) ±1,6 μg/kg (10 μg/kg) ±2,6 μg/kg (15 μg/kg) 5-hidroksifluniksin / 5-hydroxyflunixin (FLU-OH): (1–20) µg/kg CCα = 1,2 μg/kg CCβ = 1,4 μg/kg ±0,32 μg/kg (1 μg/kg) ±0,38 μg/kg (2 μg/kg) ±0,38 μg/kg (3 μg/kg) oksifenilbutazon / oxyphenbutazon (OBZ): (20–200) µg/kg e CCα = 25 μg/kg CCβ = 29 μg/kg ±6,2 μg/kg (20 μg/kg) ±8,6 μg/kg (40 μg/kg) ±7,2 μg/kg (60 μg/kg) diklofenak / diclofenac (DCL): (10–200) µg/kg CCα = 12 μg/kg CCβ = 13 μg/kg ±3,1 μg/kg (10 μg/kg) ±3,2 μg/kg (20 μg/kg) ±3,5 μg/kg (30 μg/kg) fenilbutazon / phenylbutazone (PBZ): (20–200) µg/kg CCα = 26 μg/kg CCβ = 33 μg/kg ±4,0 μg/kg (20 μg/kg) ±8,0 μg/kg (40 μg/kg) ±5,5 μg/kg (60 μg/kg)

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

meso / meat: karprofen / carprofen CPF): (250–2500) µg/kg CCα = 592 μg/kg CCβ = 684 μg/kg ±32 μg/kg (250 μg/kg) ±127 μg/kg (500 μg/kg) ±262 μg/kg (750 μg/kg) ketoprofen / ketoprofen (KPF): (2,5–25) µg/kg CCα = 2,71 μg/kg CCβ = 2,91μg/kg ±0,46 μg/kg (2,5 μg/kg) ±0,75 μg/kg (5 μg/kg) ±1,91 μg/kg (7,5 μg/kg) fluniksin / flunixin (FLU): (5–50) µg/kg CCα = 12,8 μg/kg CCβ = 15,7 μg/kg ±2,0 μg/kg (5 μg/kg) ±3,8 μg/kg (10 μg/kg) ±4,5 μg/kg (15 μg/kg) tolfenamska kislina / tolfenamic acid (TFA): (25–250) µg/kg CCα = 68,5 μg/kg CCβ = 87,0 μg/kg ±16 μg/kg (25 μg/kg) ±23 μg/kg (50 μg/kg) ±35 μg/kg (75 μg/kg) meloksikam / meloxicam (MLX): (10–100) µg/kg CCα = 24,7 μg/kg CCβ = 29,4 μg/kg ±3,2 μg/kg (10 μg/kg) ±6,9 μg/kg (20 μg/kg) ±10,6 μg/kg (30 μg/kg) 5-hidroksifluniksin / 5-hydroxyflunixin (FLU-OH): (2,5–25) µg/kg CCα = 3,53 μg/kg CCβ = 4,55 μg/kg ±1,75 μg/kg (2,5 μg/kg) ±1,72 μg/kg (5 μg/kg) ±2,15 μg/kg (7,5 μg/kg) oksifenilbutazon / oxyphenbutazon (OBZ): (2,5–25) µg/kg e CCα = 3,06 μg/kg CCβ = 3,61 μg/kg ±0,86 μg/kg (2,5 μg/kg) ±2,12 μg/kg (5 μg/kg) ±2,34 μg/kg (7,5 μg/kg) diklofenak / diclofenac (DCL): (2,5–25) µg/kg CCα = 2,98 μg/kg CCβ = 3,46 μg/kg ±0,72 μg/kg (2,5 μg/kg) ±1,37 μg/kg (5 μg/kg) ±2,63 μg/kg (7,5 μg/kg) fenilbutazon / phenylbutazone (PBZ): (2,5–25) µg/kg CCα = 3,00 μg/kg CCβ = 3,43 μg/kg ±0,81 μg/kg (2,5 μg/kg) ±0,97 μg/kg (5 μg/kg) ±2,15 μg/kg (7,5 μg/kg)

DA / YES


Določanje ostankov kokcidiostatikov z LC-MS/MS Determining residues of coccidiostats with LC-MS/MS

jajca / eggs potrditvena metoda / confirmatory method: amprol / amprolium (AMP): (5–50) µg/kg CCα = 11 µg/kg CCβ = 13 µg/kg ±3,5 μg/kg (5 μg/kg) ±1,7 μg/kg (10 μg/kg)

DA / YES DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

±3,1 μg/kg (15 μg/kg) klopidol / clopidol CL): (5–50) µg/kg CCα = 11 µg/kg CCβ = 13 µg/kg ±1,9 μg/kg (5 μg/kg) ±2,5 μg/kg (10 μg/kg) ±3,8 μg/kg (15 μg/kg) dekokvinat / decoquinate (DEQ):: (10–100) µg/kg CCα = 23 µg/kg CCβ = 26 µg/kg ±4,2 μg/kg (10 μg/kg) ±3,4 μg/kg (20 μg/kg) ±8,1 μg/kg (30 μg/kg) diklazuril / diclazuril (DI): (1–10) µg/kg CCα = 2,2 µg/kg CCβ = 2,5 µg/kg ±0,29 μg/kg (1 μg/kg) ±0,32 μg/kg (2 μg/kg) ±0,49 μg/kg (3 μg/kg) nikarbazin / nicarbazin (DNC): (50–500) µg/kg CCα = 107 µg/kg CCβ = 114 µg/kg ±12,0 μg/kg (50 μg/kg) ±10,0 μg/kg (100 μg/kg) ±12,5 μg/kg (150 μg/kg) halofuginon / halofuginone (HAL): (3–30) µg/kg CCα = 6,8 µg/kg CCβ = 7,7 µg/kg ±1,49 μg/kg (3 μg/kg) ±1,01 μg/kg (6 μg/kg) ±1,87 μg/kg (9 μg/kg) lasalocid / lasalocid (LA) (75–750) µg/kg CCα = 172 μg/kg CCβ = 196 μg/kg ±29,0 μg/kg (75 μg/kg) ±27,9 μg/kg (150 μg/kg) ±50,7 μg/kg (225 μg/kg) maduramicin / maduramicin (MA): (1–10) µg/kg CCα = 2,1 µg/kg CCβ = 2,3 µg/kg ±0,27 μg/kg (1 μg/kg) ±0,28 μg/kg (2 μg/kg) ±0,35 μg/kg (3 μg/kg) monensin / monensin (MO): (1,5–15) µg/kg CCα = 3,2 µg/kg CCβ = 3,5 µg/kg ±0,30 μg/kg (1,5 μg/kg) ±0,33 μg/kg (3 μg/kg) ±0,51 μg/kg (4,5 μg/kg) narasin / narasin (NA): (1–10) µg/kg CCα = 2,3 µg/kg CCβ = 2,7 µg/kg ±0,28 μg/kg (1 μg /kg) ±0,40 μg/kg (2 μg /kg) ±0,39 μg/kg (3 μg /kg) robenidin / robenidin (ROB): (12,5–125) µg/kg CCα = 29 µg/kg CCβ = 33 µg/kg ±3,5 μg/kg (12,5 μg/kg) ±4,6 μg/kg (25 μg/kg) ±10,9 μg/kg (37,5 μg/kg)





number




Akreditirano* Da/Ne

Accredited Yes/No

salinomicin / salinomicin (SA): (1,5–15) µg/kg CCα = 3,6 µg/kg CCβ = 4,3 µg/kg ±0,72 μg/kg (1,5 μg/kg) ±0,98 μg/kg (3 μg/kg) ±1,13 μg/kg (4,5 μg/kg) semduramicin / semduramicin (SED): (1,0–10) µg/kg CCα = 2,2 µg/kg CCβ = 2,4 µg/kg ±0,35 μg/kg (1 μg/kg) ±0,48 μg/kg (2 μg/kg) ±0,34 μg/kg (3 μg/kg) presejalna metoda / screening method: amprol / amprolium (AMP): CCβ = 10 µg/kg klopidol / clopidol CL): CCβ = 10 µg/kg dekokvinat / decoquinate (DEQ): CCβ = 10 µg/kg diklazuril / diclazuril (DI): CCβ = 1,0 µg/kg nikarbazin / nicarbazin (DNC): CCβ = 50 µg/kg halofuginon / halofuginone (HAL): CCβ = 3,0 µg/kg lasalocid / lasalocid (LA): CCβ = 75 μg/kg maduramicin / maduramicin (MA): CCβ = 1,0 µg/kg monensin / monensin (MO): CCβ = 1,5 µg/kg narasin / narasin (NA): CCβ = 1,0 µg/kg robenidin / robenidin (ROB): CCβ = 25 µg/kg salinomicin / salinomicin (SA): CCβ = 1,5 µg/kg semduramicin / semduramicin (SED): CCβ = 1,0 µg/kg jetra / liver potrditvena metoda / confirmatory method: amprol / amprolium (AMP): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other (10–100) µg/kg CCα = 25 µg/kg CCβ = 29 µg/kg ±3,3 μg/kg (10 μg/kg) ±6,1 μg/kg (20 μg/kg) ±7,8 μg/kg (30 μg/kg) klopidol / clopidol CL): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other (10–100) µg/kg CCα = 26 µg/kg CCβ = 32 µg/kg ±4,6 μg/kg (10 μg/kg) ±7,6 μg/kg (20 μg/kg) ±8,2 μg/kg (30 μg/kg) dekokvinat / decoquinate (DEQ): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other (10–100) µg/kg CCα = 25 µg/kg CCβ = 29 µg/kg ±3,9 μg/kg (10 μg/kg) ±5,8 μg/kg (20 μg/kg) ±6,1 μg/kg (30 μg/kg) diklazuril / diclazuril (DI): purani, govedo, svinjina, ostalo / poultry, bovine, porcine, other (20–200) µg/kg






number




Akreditirano* Da/Ne

Accredited Yes/No

CCα = 48 µg/kg CCβ = 57 µg/kg ±4,8 μg/kg (20 μg/kg) ±10,1 μg/kg (40 μg/kg) ±16,9 μg/kg (60 μg/kg) piščanci, kunci / broiler, rabbit (250–2000) µg/kg CCα = 551 µg/kg CCβ = 602 µg/kg ±35,6 μg/kg (250 μg/kg) ±125,5 μg/kg (500 μg/kg) ±136,4 μg/kg (1000 μg/kg) nikarbazin / nicarbazin (DNC): purani, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other (50–500) µg/kg CCα = 107 µg/kg CCβ = 114 µg/kg ±12,5 μg/kg (50 μg/kg) ±12,9 μg/kg (100 μg/kg) ±24,3 μg/kg (150 μg/kg) piščanci / broiler (250–2000) µg/kg CCα = 538 µg/kg CCβ = 575 µg/kg ±75,3 μg/kg (250 μg/kg) ±92,4 μg/kg (500 μg/kg) ±121,5 μg/kg (1000 μg/kg) halofuginon / halofuginone (HAL): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other (15–150) µg/kg CCα = 34 µg/kg CCβ = 38 µg/kg ±4,9 μg/kg (15 μg/kg) ±5,0 μg/kg (30 μg/kg) ±13,4 μg/kg (45 μg/kg) lasalocid / lasalocid (LA): svinjina, kunci, ostalo / porcine, rabbit, other (50–400) µg/kg CCα = 63 μg/kg CCβ = 76 μg/kg ±8,1 μg/kg (25 μg/kg) ±16,0 μg/kg (50 μg/kg) ±25,5 μg/kg (100 μg/kg) perutnina, govedo / poultry, bovine (25–250) µg/kg CCα = 113 μg/kg CCβ = 127 μg/kg ±9,4 μg/kg (50 μg/kg) ±20,1 μg/kg (100 μg/kg) ±22,5 μg/kg (150 μg/kg) maduramicin / maduramicin (MA): purani, govedo, svinjina, kunci, ostalo / turkey, bovine, porcine, rabbit, other (1–10) µg/kg CCα = 2,6 µg/kg CCβ = 3,2 µg/kg ±0,38 μg/kg (1 μg/kg) ±0,71 μg/kg (2 μg/kg) ±0,67 μg/kg (3 μg/kg) piščanci / broiler: (75–600) µg/kg CCα = 175 μg/kg CCβ = 200 μg/kg ±12,7 μg/kg (75 μg/kg) ±30,7 μg/kg (150 μg/kg) ±45,4 μg/kg (225 μg/kg) monensin / monensin (MO): perutnina, svinjina, kunci, ostalo / poultry, porcine, rabbit, other: (4–40) µg/kg CCα = 11 µg/kg CCβ = 14 µg/kg ±1,5 μg/kg (4 μg/kg) ±3,4 μg/kg (8 μg/kg) ±2,8 μg/kg (12 μg/kg)





number




Akreditirano* Da/Ne

Accredited Yes/No

govedo / bovine: (15–90) µg/kg CCα = 35 μg/kg CCβ = 41 μg/kg ±2,6 μg/kg (15 μg/kg) ±7,5 μg/kg (30 μg/kg) ±14,7 μg/kg (45 μg/kg) narasin / narasin (NA): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other (25–250) µg/kg CCα = 66 µg/kg CCβ = 83 µg/kg ±11,4 μg/kg (25 μg/kg) ±19,8 μg/kg (50 μg/kg) ±33,8 μg/kg (75 μg/kg) robenidin / robenidin (ROB): govedo, svinjina, kunci, ostalo / bovine, porcine, rabbit, other: (25–250) µg/kg CCα = 29 µg/kg CCβ = 33 µg/kg ±16,8 μg/kg (25 μg/kg) ±41,0 μg/kg (50 μg/kg) ±44,3 μg/kg (75 μg/kg) perutnina / poultry: (200–1600) µg/kg CCα = 491 μg/kg CCβ = 583 μg/kg ±92,4 μg/kg (200 μg/kg) ±121,3 μg/kg (400 μg/kg) ±243,7 μg/kg (600 μg/kg) salinomicin / salinomicin (SA): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: (2,5–25) µg/kg CCα = 7,1 µg/kg CCβ = 9,3 µg/kg ±1,50 μg/kg (2,5 μg/kg) ±3,09 μg/kg (5 μg/kg) ±2,95 μg/kg (7,5 μg/kg) semduramicin / semduramicin (SED): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: (1,0–10) µg/kg CCα = 2,7 µg/kg CCβ = 3,4 µg/kg ±0,49 μg/kg (1 μg/kg) ±0,87 μg/kg (2 μg/kg) ±0,95 μg/kg (3 μg/kg) presejalna metoda / screening method: amprol / amprolium (AMP): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 20 µg/kg clopidol / clopidol (CL): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 20 µg/kg dekokvinat / decoquinate (DEQ): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 10 µg/kg diklazuril / diclazuril (DI): purani, govedo, svinjina, ostalo / turkey, bovine, porcine, other: CCβ = 20 µg/kg piščanci, kunci / broiler, rabbit: CCβ = 500 µg/kg nikarbazin / nicarbazin (DNC): purani, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other:

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

CCβ = 50 µg/kg piščanci / broiler: CCβ = 500 µg/kg halofuginon / halofuginone (HAL): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 15 µg/kg lasalocid / lasalocid (LA): svinjina, kunci, ostalo / porcine, rabbit, other: CCβ = 25 μg/kg perutnina, govedo / poultry, bovine CCβ = 50 μg/kg maduramicin / maduramicin (MA): purani, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 1,0 µg/kg piščanci / broiler: CCβ = 75 µg/kg monensin / monensin (MO): perutnina, svinjina, kunci, ostalo / poultry, porcine, rabbit, other: CCβ = 4,0 µg/kg govedo / bovine: CCβ = 15 µg/kg narasin / narasin (NA): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 25 µg/kg robenidin / robenidin (ROB): govedo, svinjina, kunci, ostalo / bovine, porcine, rabbit, other: CCβ = 25 µg/kg perutnina / poultry: CCβ = 200 μg/kg salinomicin / salinomicin (SA): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 2,5 µg/kg semduramicin / / semduramicin (SED): perutnina, govedo, svinjina, kunci, ostalo / poultry, bovine, porcine, rabbit, other: CCβ = 1,0 µg/kg


Postopek za določanje vsebnosti zaostankov dapsona v vzorcih mesa, jajc,urina in mleka, presejalna metoda (HPLC-UV) Procedure for the determination residues of dapson in meet, eggs urin and milk, screening method (HPLC-UV)

jajca / eggs CCβ = 2,5 µg/kg meso / meat CCβ = 2,5 µg/kg mleko / milk CCβ = 2,5 µg/kg



Ugotavljanje in potrditev ostankov tetraciklinov z LC-MS/MS Identification and confirmation of Tetracyclines residues with Lc-MS/MS

mleko / milk klortetraciklin / chlortetracycline (CTC): (5–200) µg/kg CCα = 112 μg/kg CCβ = 124 μg/kg ±3,7 μg/kg (50 μg/kg) ±16,0 μg/kg (100 μg/kg) ±21,6 μg/kg (150 μg/kg) oksitetraciklin / oxytetracycline (OTC): (5–200) µg/kg CCα = 110 μg/kg CCβ = 120 μg/kg ±6,6 μg/kg (50 μg/kg) ±12,7 μg/kg (100 μg/kg) ±34,2 μg/kg (150 μg/kg) tetraciklin / tetracycline (TC): (5–200) µg/kg CCα = 110 μg/kg CCβ = 120 μg/kg ±5,6 μg/kg (50 μg/kg) ±12,9 μg/kg (100 μg/kg) ±13,2 μg/kg (150 μg/kg)

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

doksiciklin / doxycycline (DC):: (5–20) µg/kg CCα = 5,8 μg/kg CCβ = 6,7 μg/kg ±1,6 μg/kg (5 μg/kg) ±1,8 μg/kg (10 μg/kg) ±3,5 μg/kg (15 μg/kg) krma/feed CTC, OTC, TC in DC: LOQ = 50 μg/kg

NE / NO


Določanje antimikrobnih substanc z UPLC-MS/MS - mleko Z UPLC-MS/MS Determination of antimicrobic substances with UPLC-MS/MS - milk

mleko / milk potrditvena metoda / confirmatory method: penicilin G / penicillin G: (2–20) µg/kg CCα = 4,3 μg/kg CCβ = 4,6 μg/kg ±0,20 μg/kg (2 μg/kg) ±0,31 μg/kg (4 μg/kg) ±0,62 μg/kg (6 μg/kg) ampicilin / ampicillin: (2–20) µg/kg CCα = 4,5 μg/kg CCβ = 4,9 μg/kg ±0,33 μg/kg (2 μg/kg) ±0,70 μg/kg (4 μg/kg) ±0,82 μg/kg (6 μg/kg) linkomicin / lincomicin: (75–750) µg/kg CCα = 155 μg/kg CCβ = 160 μg/kg ±6,0 μg/kg (75 μg/kg) ±8,7 μg/kg (150 μg/kg) ±12,0 μg/kg (225 μg/kg) kloksacilin / cloxacillin: (15–150) µg/kg CCα = 34 μg/kg CCβ = 37 μg/kg ±2,9 μg/kg (15 μg/kg) ±4,6 μg/kg (30 μg/kg) ±6,9 μg/kg (45 μg/kg) nafcilin / nafcillin: (15–150) µg/kg CCα = 33 μg/kg CCβ = 36 μg/kg ±1,5 μg/kg (15 μg/kg) ±3,1 μg/kg (30 μg/kg) ±4,0 μg/kg (45 μg/kg) novobiocin / novobiocin: (25–250) µg/kg CCα = 56 μg/kg CCβ = 62 μg/kg ±4,6 μg/kg (25 μg/kg) ±11 μg/kg (50 μg/kg) ±17 μg/kg (75 μg/kg) cefoperazon / cefoperazone: (25–250) µg/kg CCα = 54 μg/kg CCβ = 58 μg/kg ±2,4 μg/kg (25 μg/kg) ±3,0 μg/kg (50 μg/kg) ±33 μg/kg (75 μg/kg) ceftiofur / ceftiofur: (50–500) µg/kg CCα = 106 μg/kg CCβ = 113 μg/kg ±3,5 μg/kg (50 μg/kg) ±8,8 μg/kg (100 μg/kg) ±14 μg/kg (150 μg/kg) eritromicin / eritromicin:: (20–200) µg/kg CCα = 42 μg/kg CCβ = 43 μg/kg

DA / YES DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

±1,4 μg/kg (20 μg/kg) ±2,2 μg/kg (40 μg/kg) ±4,4 μg/kg (60 μg/kg) presejalna metoda / screening method: amoksicilin / amoksicillin: CCβ = 2,0 μg/kg krma/feed linkomicin / lincomicin: LOQ = 75 μg/kg

DA / YES NE / NO


Določanje kovin v živilih z ICP – MS Determination of metals in food with ICP-MS

mleko / milk: Pb: (0,007–1,000) mg/kg LOD Pb = 0,002 mg/kg LOQ Pb = 0,007 mg/kg ± 20 % mg/kg (0,007–0,100 mg/kg) ± 15 % mg/kg (0,101–1,000 mg/kg) Cd: (0,002-0,500) mg/kg LOD Cd = 0,0007 mg/kg LOQ Cd = 0,002 mg/kg ± 20 % (0,002–0,020 mg/kg) ± 10 % (0,021–0,500 mg/kg) meso / meat Pb: (0,01–5,000) mg/kg LOD Pb = 0,003 mg/kg LOQ Pb = 0,010 mg/kg ± 15 % mg/kg (0,010–0,100 mg/kg) ± 10 % mg/kg (0,101–5,000 mg/kg) Cd: (0,005–2,500) mg/kg LOD Cd = 0,0015 mg/kg LOQ Cd = 0,005 mg/kg ± 25 % (0,005–0,020 mg/kg) ± 10 % (0,021–2,500 mg/kg) jetra in ledvica / liver and kidney Pb: (0,06–4,00) mg/kg LOD Pb = 0,018 mg/kg LOQ Pb = 0,06 mg/kg ± 20 % mg/kg (0,06–0,100 mg/kg) ± 10 % mg/kg (0,101–4,00 mg/kg) Cd: (0,01–2,00) mg/kg LOD Cd = 0,003 mg/kg LOQ Cd = 0,01 mg/kg ± 20 % (0,01–0,050 mg/kg) ± 10 % (0,051–2,00 mg/kg

DA/YES DA/YES DA/YES

20. SOP 462, verzija 3 Ugotavljanje ostankov pomirjeval Determination of sedatives screening method

jajca / eggs Klorpromazin / Chlorpromazin: CC = 2,5 μg/kg Karazolol / Carazolol: CC = 0,5 μg/kg urin / urine Klorpromazin / Chlorpromazin: CC = 2,5 μg/kg Karazolol / Carazolol: CC = 0,5 μg/kg Azaperon / Azaperon: CC = 2,5 μg/kg Azaperol / Azaperol: CC = 2,5 μg/kg ledvice / kidney Klorpromazin / Chlorpromazin: CC = 2,5 μg/kg

DA / YES DA / YES NE / NO





number




Akreditirano* Da/Ne

Accredited Yes/No

Karazolol / Carazolol: CC = 0,5 μg/kg Azaperon / Azaperon: CC = 2,5 μg/kg Azaperol / Azaperol: CC = 2,5 μg/kg Acepromazine / Acepromazine: CC = 25 μg/kg Propiopromazin / Propiopromazine: CC = 25 μg/kg mleko / milk: Klorpromazin / Chlorpromazin: CC = 2,5 μg/kg Karazolol / Carazolol: CC = 0,5 μg/kg

NE / NO

21. SOP 296, verzija 3 interna metoda / in-house method

Ugotavljanje ostankov kloramfenikola, florfenikola in tiamfenikola z LC-MS/MS Determination of chloramphenicol, florfenicole and tiamphenicol residues with LC-MS/MS

potrditvena metoda / confirmatory method: jajca / eggs kloramfenikol / chloramphenicol (CAP) (0,1–10) μg/kg CCα = 0,14 μg/kg CCβ = 0,18 μg/kg ±0,05 μg/kg (0,1 μg/kg) ±0,10 μg/kg (0,3 μg/kg) ±0,12 μg/kg (0,4 μg/kg) florfenikol / florfenicol (FF) (0,5–50) μg/kg CCα = 1,83 μg/kg CCβ = 2,16 μg/kg ±0,15 μg/kg (0,5 μg/kg) ±0,35 μg/kg (1,5 μg/kg) ±0,69 μg/kg (5,0 μg/kg) tiamfenikol / tiamphenicol (TP) (1–100) μg/kg CCα = 3,62 μg/kg CCβ = 4,24 μg/kg ±0,45 μg/kg (1 μg/kg) ±0,69 μg/kg (3 μg/kg) ±2,05 μg/kg (10 μg/kg) mleko / milk kloramfenikol / chloramphenicol (CAP) (0.1–10) μg/kg CCα = 0.15 μg/kg CCβ = 0.20 μg/kg ±0.06 μg/kg (0.1 μg/kg) ±0.09 μg/kg (0.3 μg/kg) ±0.11 μg/kg (0.4 μg/kg) florfenikol / florfenicol (FF) (0.5–50) μg/kg CCα = 2.17 μg/kg CCβ = 2.48 μg/kg ±0.25 μg/kg (0.5 μg/kg) ±0.67 μg/kg (1,5 μg/kg) ±0.96 μg/kg (5.0 μg/kg) tiamfenikol / tiamphenicol (TP) (1–100) μg/kg CCα = 59 μg/kg CCβ = 69 μg/kg ±5.4 μg/kg (25 μg/kg) ±10.3 μg/kg (50 μg/kg) ±14.4 μg/kg (75 μg/kg) presejalna metoda / screening method: jajca / eggs kloramfenikol / chloramphenicol (CAP) CCβ = 0,1 μg/kg florfenikol / florfenicol (FF) CCβ = 0,5 μg/kg florfenikol amin / florfenicol amine (FFA) CCβ = 0,5 μg/kg

DA / YES DA / YES DA / YES DA / YES DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

tiamfenikol / tiamphenicol (TP) CCβ = 1,0 μg/kg mleko / milk kloramfenikol / chloramphenicol (CAP) CCβ = 0.1 μg/kg florfenikol / florfenicol (FF) CCβ = 0.5 μg/kg florfenikol amin / florfenicol amine (FFA) CCβ = 0.5 μg/kg tiamfenikol / tiamphenicol (TP) CCβ = 25 μg/kg

DA / YES


Določanje sulfonamidov in dapsona v mleku (s HPLC - DAD) Determining sulfonamides and dapsone in milk (by HPLC - DAD)

mleko / milk potrditvena metoda / confirmatory method: (25–150) μg/L sulfatiazol / sulfathiazole (STZ) ±11 μg/l (100 μg/kg) CCα = 109 μg/kg CCβ = 117 μg/kg sulfakloropiridazin / sulfachloropyridazine (SCP) ±9 μg/l (100 μg/kg) CCα = 107 μg/kg CCβ = 114 μg/kg sulfadimetoksin / sulfadimetoxine (SDM) ±8 μg/l (100 μg/kg) CCα = 107 μg/kg CCβ = 113 μg/kg sulfadiazin / sulfadiazne (SDZ) ±9 μg/l (100 μg/kg) CCα = 107 μg/kg CCβ = 115 μg/kg sulfamonometoksin / sulfamonometoxine (SMM) ±9 μg/l (100 μg/kg) CCα = 108 μg/kg CCβ = 115 μg/kg sulfapiridin / sulfapyridine (SPY) ±12 μg/l (100 μg/kg) CCα = 110 μg/kg CCβ = 120 μg/kg sulfametazin / sulfametazine (SMZ) ±11 μg/l (100 μg/kg) CCα = 109 μg/kg CCβ = 118 μg/kg sulfamerazin / sulfamerazine (SRZ) ±10 μg/l (100 μg/kg) CCα = 109 μg/kg CCβ = 117 μg/kg sulfakinoksalin / sulfaquinoxaline (SQX) ±10 μg/l (100 μg/kg) CCα = 109 μg/kg CCβ = 117 μg/kg sulfisoksazol / sulfisoxazole (SFX) ±11 μg/l (100 μg/kg) CCα = 109 μg/kg CCβ = 118 μg/kg presejalna metoda / screening method: dapson / dapsone (DP) CCβ = 2,5 µg/kg



Določanje sulfonamidov v medu s HPLC – fluorescenčno detekcijo Determining sulfonamides in honey by HPLC – fluorescence detection

med / honey (5–50) μg/kg sulfanilamide / sulfanilamide (SAD) ±7 μg/kg (10 μg/kg) CCα = 8 μg/kg CCβ = 11 μg/kg sulfatiazol / sulfathiazole (STZ) ±4 μg/kg (10 μg/kg) CCα = 8 μg/kg CCβ = 11 μg/kg

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

sulfapiridin / sulfapyridine (SPY) ±4 μg/kg (10 μg/kg) CCα = 9 μg/kg CCβ = 13 μg/kg sulfadiazine / sulfadiazine (SDZ) ±3μg/kg (10 μg/kg) CCα = 6 μg/kg CCβ = 7 μg/kg sulfamerazin / sulfamerazine (SRZ)) ±4 μg/kg (10 μg/kg) CCα = 9 μg/kg CCβ = 13 μg/kg sulfametazin / sulfamethazine (SMZ) ±10 μg/kg (10 μg/kg) CCα = 8 μg/kg CCβ = 12 μg/kg sulfamonometoksin / sulfamonomethoxine (SMM) ±5 μg/kg (10 μg/kg) CCα = 8 μg/kg CCβ = 10 μg/kg sulfametizol / sulfamethizole (SMTZ) ±3μg/kg (10 μg/kg) CCα = 7 μg/kg CCβ = 8 μg/kg sulfametoksazol / sulfamethoxazole (SMX) ±3μg/kg (10 μg/kg) CCα = 6 μg/kg CCβ = 7 μg/kg sulfisoksazol / sulfisoxasole (SFX) ±3μg/kg (10 μg/kg) CCα = 6 μg/kg CCβ = 7 μg/kg sulfadimetoksin / sulfadimethoxine (SDM) ±1μg/kg (10 μg/kg) CCα = 6 μg/kg CCβ = 7 μg/kg


Določevanje malahitnega in levkomalahitnega zelenila v ribah s HPLC z UV-VIS in FLD Detection of malachite and leuco-malachite green in fish with HPLC withUV-VIS and FLD

ribe / fish malahitno zelenilo / malahit green 0,5–10 μg/kg CCα = 0,6 μg/kg CCβ = 1,0 μg/kg ± 0,32 μg/kg (2 g/kg) ± 0,44 μg/kg (3 g/kg) ± 0,57 μg/kg (4 g/kg) leukomalahitno zelenilo / leucomalahit green 0,5–10 μg/kg CCα = 0,5 μg/kg CCβ = 0,9 μg/kg ± 0,46 μg/kg (2 g/kg) ± 0,44 μg/kg (3 g/kg) ± 0,36 μg/kg (4 g/kg) kristal vijolično LOQ = 1,0 μg/kg leukokristal vijolično LOQ = 1,0 μg/kg briljant zeleno LOQ = 1,0 μg/kg

DA / YES Da / Yes Da / Yes Ne / No Ne / No Ne / No


Ugotavljanje ostankov azaperona s HPLC - fluorescenčno detekcijo Detection of residues of azaperone by HPLC - fluorescence detection

ledvice / kidneys azaperon / azaperone (AZN) (10–150) g/kg CCα = 112 μg/kg CCβ = 122 μg/kg ± 9 μg/kg (50 g/kg) ± 20 μg/kg (100 g/kg) ± 24 μg/kg (150 g/kg)

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

azaperol / azaperole (AZL) (5–150) g/kg CCα = 111 μg/kg CCβ = 122 μg/kg ± 7 μg/kg (50 g/kg) ± 17 μg/kg (100 g/kg) ± 18 μg/kg (150 g/kg)

26. ISO 13730:1996 Modificirana/Modified

Spektrofotometrična metoda določanja polifosfatov v mesnih izdelkih Meat and meat products – Determination of total phosphorus content – Spectrometric method SOP 148, verzija 6

mesni izdelki / meat products P2O5: 1 – 7 g/kg ± 5 %

sušene mesnine / dry meat products ± 7 %

DA / YES DA / YES


Določanje vsebnosti hidroksiprolina – meso in mesni izdelki - spektrofotometrična metoda Meat and meat products – Determination of hydroxyproline content SOP 164, verzija 2

meso in mesni izdelki / meat and meat products (0,05–0,5) g/100g Vzorci z > 0,1 g/100 g hidroksiprolina ± 12 % Vzorci z ≤ 0,1 g/100g hidroksiprolina ± 18 %

DA / YES


Določanje vsebnosti vode v mesu in mesnih izdelkih - gravimetrična metoda, sušenje Meat and meat products – Determination of moisture content

SOP 170, verzija 5

meso in mesni izdelki / meat and meat products (20–80) g/100g ± 1,0 %

sušene mesnine / dry meat products ± 2,3 %

DA / YES DA / YES


Določanje vsebnosti celokupne maščobe v mesu in mesnih izdelkih - gravimetrična metoda, kislinska hidroliza, ekstrakcija Meat and meat products – Determination of total fat content SOP 179, verzija 4

meso in mesni izdelki / meat and meat products (1 – 50) g/100g Vzorci s vsebnostjo maščobe < 6 g/100 g ± 14 % Vzorci s vsebnostjo maščobe ≥ 6 g/100 g ± 5 %

DA / YES

30. AOAC official method 1995 16ed 973.31 (Ch 93 p 8-9) Modificirana/Modified

Določanje vsebnosti nitritov v mesnih izdelkih - Spektrofotometrična metoda Nitrites in cured meat – Colorimetric method SOP 2, verzija 7

mesni izdelki / meat products (5–140) mg/kg NaNO2: (5 – 10) mg/kg ± 50 % (10 – 30) mg/kg ± 20 % (30 – 140) mg/kg ± 13 %

DA / YES

31. ISO 936:1998

Določanje vsebnosti celokupnega pepela v mesu in mesnih izdelkih - gravimetrična, sežig Meat and meat products -- Determination of total ash SOP 281, verzija 2

meso in mesni izdelki / meat and meat products (1–7,5) g/100g ± 3,6 %

DA / YES


Ugotavljanje prisotnosti zaviralnih substanc z mikrobiološko metodo Detection of antimicrobial residues with microbiological method

mleko / milk tetraciklini / tetracyclines tetraciklin / tetracycline (TC) CCβ = 100 µg/kg klortetraciklin / chlortetracycline (CTC) CCβ = 80 µg/kg oksitetraciklin / oxytetracycline (OTC) CCβ = 100 µg/kg doksiciklin / doxycycline (DC) CCβ = 30 µg/kg aminoglikozidi / aminoglycosides streptomicin / streptomycin CCβ = 200 µg/kg dihidrostreptomicin / dihydrostreptomycin CCβ = 200 µg/kg gentamicin / gentamycin CCβ = 100 µg/kg neomicin / neomycin CCβ = 100 µg/kg rifaksimin / rifaximin CCβ = 50 µg/kg

DA / YES Da / Yes Da / Yes





number




Akreditirano* Da/Ne

Accredited Yes/No

kanamicin / kanamycin CCβ = 300 µg/kg (very low sensitivity) meso / meat tetraciklini / tetracyclines tetraciklin / tetracycline (TC) CCβ = 50 µg/kg klortetraciklin / chlortetracycline (CTC) CCβ = 30 µg/kg oksitetraciklin / oxytetracycline (OTC) CCβ = 70 µg/kg doksiciklin / doxycycline (DC) CCβ = 30 µg/kg aminoglikozidi / aminoglycosides apramicin / apramycin CCβ = 800 µg/kg streptomicin / streptomycin CCβ = 500 µg/kg dihidrostreptomicin / dihydrostreptomycin CCβ = 500 µg/kg gentamicin / gentamycin CCβ = 100 µg/kg neomicin / neomycin CCβ = 250 µg/kg rifaksimin / rifaximin CCβ = 150 µg/kg kanamicin / kanamycin CCβ = 200 µg/kg paramomicin / paramomycin CCβ = 250 µg/kg jajca / eggs tetraciklini / tetracyclines tetraciklin / tetracycline (TC) CCβ = 200 µg/kg klortetraciklin / chlortetracycline (CTC) CCβ = 80 µg/kg oksitetraciklin / oxytetracycline (OTC) CCβ = 200 µg/kg doksiciklin / doxycycline (DC) CCβ = 80 µg/kg

DA / YES Da / Yes Da / Yes NE / NO Ne/ No


Preiskava na kinolone z mikrobiološko metodo Microbiological screening method for quinolones

jajca / eggs: danofloksacin / danofloxacin (DAN) CCβ = 10 µg/kg enrofloksacin / enrofloxacin (EFC) marbofloksacin / marbofloxacin (MAR) sarafloksacin / sarafloxacin (SAR) CCβ = 15 µg/kg orbifloksacin / orbifloxacin (ORB) CCβ = 25 µg/kg difloksacin / difloxacin (DIF) CCβ = 60 µg/kg flumekvin / flumequin (FLU) oksolinska kislina / oxolinic acid (OXO) CCβ = 300 µg/kg meso / meat: difloksacin / difloxacin (DIF) enrofloksacin / enrofloxacin (EFC) marbofloksacin / marbofloxacin (MAR) oksolinska kislina / oxolinic acid (OXO) orbifloksacin / orbifloxacin (ORB) CCβ = 50 µg/kg flumekvin / flumequin (FLU) danofloksacin / danofloxacin (DAN) sarafloksacin / sarafloxacin (SAR) CCβ = 100 µg/kg

DA / YES DA / YES

34. ISO 6888-2:1999 Amendment 1: 2003

Preiskava na koagulaza pozitivne stafilokoke Microbiology of food and animal feeding stuffs – Horizontal method for the enumeration of coagulase-positive staphylococci (S. aureus and other species) by

meso in mesni izdelki / meat and meat products LOD 10 CFU/g ± 0,12 log10 CFU/g

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

– Part 2: Technique using rabbit plasma fibrinogen agar medium SOP 204, verzija 2

mleko in mlečni izdelki / milk and milk products LOD 10 CFU/g (ml) ± 0,19 log10 CFU/g

DA / YES

35. ISO 4833-1: 2013

Določanje skupnega števila mikroorganizmov (tehnika štetja kolonij na ploščah inkubiranih pri 30 oC) Microbiology of the food chain – Horizontal method for the enumeration of microorganisms Part 1: Colony count at 30 oC by the pour plate technique SOP 206, verzija 4

meso in mesni izdelki / meat and meat products LOD = 10 CFU/g ±0,13 log10 CFU/g mleko in mlečni izdelki / milk and milk products LOD = 10 CFU/g (ml) ±0,08 log10 CFU/g jajca in jajčni izdelki / eggs and eggs products LOD = 10 CFU/g (ml) ±0,17 log10 CFU/g (ml) brisi okolja / environmental swabs LOD = 5 CFU/površina ±0,17 log10

DA / YES DA / YES DA / YES DA/YES

36. ISO 6579-1:2017

Mikrobiologija v prehranski verigi -Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella – 1. del: Ugotavljanje prisotnosti Salmonella spp. Microbiology of the food chain - Horizontal method for the detection and enumeration of Salmonella spp. SOP 400, verzija 2/version 2

meso in mesni izdelki / meat and meat products LOD = (1–5) CFU/25g mleko in mlečni izdelki / milk and milk products LOD = (1–5) CFU/25ml jajca in jajčni izdelki / eggs and egg products LOD = (5–10) CFU/25 g


37. ISO 11290-1: 2017

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 1. del: Ugotavljanje prisotnosti Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria spp.- Part 1: Detection method SOP 162, verzija 5

meso in mesni izdelki / meat and meat products LOD = 5 CFU/25 g mleko in mlečni izdelki / milk and milk products LOD = 5 CFU/25g(ml) brisi okolja / environmental swabs LOD = 5-10 CFU/površina


38. ISO 10272-1: 2006 Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Campylobacter spp. -1.del: Metoda za ugotavljanje prisotnosti Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method SOP 187, verzija 3

meso in mesni izdelki / meat and meat products LOD = 5–10 CFU/10g mleko / milk LOD = 5-10 CFU/10g

DA / YES DA / YES

39. ISO 10272-2 : 2017

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Campylobacter spp.- 2.del: Tehnika štetja kolonij Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 2 : Colony-count technique SOP 385 verzija 3

meso in mesni izdelki / meat and meat products LOD = 10 CFU/g ± 0,28 log10 CFU/g

DA / YES

40. ISO 11290-2: 2017

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 2.del: Ugotavljanje števila Microbiology of the food chain - Horizontal method for detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method SOP 314, verzija 3

meso in mesni izdelki / meat and meat products LOD = 10 CFU/g ± 0,19 log10 CFU/g mleko in mlečni izdelki / milk and milk products LOD = 10 CFU/g (ml) ±0,13 log10 CFU/g

DA / YES DA / YES

41. ISO 16649-2: 2001 Določanje števila β glukuronidaza pozitivnih bakterij Escherichia coli Kvantitativna metoda Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of β glukuronidase-positive Escherichia coli Part 2: Colony count technique at 44oC SOP 315, verzija 2

meso in mesni izdelki / meat and meat products LOD = 10 CFU/g ±0,18log10CFU/g mleko in mlečni izdelki / milk and milk products LOD = 10 CFU/g (ml) ±0,24 log10 CFU/g (tekočine,liquids) ±0,30 log10 CFU/g (trdne snovi, solid milk products)

DA / YES DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

42. ISO 16649-3: 2015

Določanje števila β–glucuronidaza- pozitivnih bakterij Escherichia coli z uporabo tehnike MPN in 5-bromo-4-chloro-3-indolyl β–D-glucuronida Microbiology of food chain - Horizontal method for the enumeration of β glucuronidase-positive Escherichia coli Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl β-D-glucuronide SOP 192, verzija 3

školjke / shellfish LOD = 18 CFU/100 ml ±0,60 log10 CFU/100 g

DA / YES

43. ISO 21528-2:2017 Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnost in števila enterobakterij - 2.del: Metoda štetja kolonij Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2: Colony count technique SOP 317, verzija 3

meso in mesni izdelki / meat and meat products LOD = 10 CFU/g ±0,13 log10 CFU/g mleko in mlečni izdelki / milk and milk products LOD = 10 CFU/g (ml) ±0,13 log10 CFU/g (tekočine in prašnate snovi / liquids and powders) ±0,21 log10 CFU/g (trdne snovi / solid milk products) jajca in jajčni izdelki / eggs and eggs products LOD = 10 CFU/g (ml) ±0,17 log10 CFU/g hrana za domače živali / pet food and feed LOD = 10 CFU/g (ml) ±0,17 log10 CFU/g

DA / YES DA / YES DA / YES DA / YES


Ugotavljanje zaviralnih substanc v mleku - DELVOTEST®SP-NT Determination of antimicrobial substances in milk - DELVOTEST®SP-NT

mleko / milk penicilin G / penicillin G CCβ = 3 µg/kg ampicilin / ampicillin CCβ = 4 µg/kg amoksicilin / amoxicillin CCβ = 4 µg/kg kloksacilin / cloxacillin CCβ = 20 µg/kg nafcilin / nafcillin not sensitive cefapirin / cefapirin not sensitive cefkvinom / cefquinome not sensitive cefalonij / cefalonium CCβ = 20 µg/kg cefoperazon / cefoperazone CCβ = 50 µg/kg ceftiofur / ceftiofur CCβ = 50 µg/kg cefazolin / cefazolin CCβ = 10 µg/kg cefaleksin / cefalexin CCβ = 70 µg/kg tetraciklin / tetracycline very low sensitivity oksitetraciklin / oxytetracycline very low sensitivity klortetraciklin / chlortetracycline not sensitive doksiciklin / doxycycline not sensitive neomicin / neomycin CCβ = 500 µg/kg streptomicin / streptomycin very low sensitivity dihidrostreptomicin / dihydrostreptomycin very low sensitivity gentamicin / gentamicin not sensitive kanamicin / kanamycin not sensitive kinoloni / quinolones not sensitive sulfatiazol / sulfathiazole

DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

CCβ = 90 µg/kg sulfadiazin / sulfadiazine very low sensitivity sulfadoksin / sulfadoxin very low sensitivity eritromicin / erythromycin very low sensitivity linkomicin / lincomycin very low sensitivity novobiocin / novobiocin very low sensitivity rifaksimin / rifaximin very low sensitivity spiramicin / spiramycin very low sensitivity tilozin / tylosin CCβ = 50 µg/kg bacitracin / bacitracin very low sensitivity


Določanje ostankov bazičnih NSAIDov (MAA, A, AA, AAA, DMAA, FAA, IPAA, 4-OH-A) z LC-MS/MS Determination of basic NSAIDs (MAA, A, AA, AAA, DMAA, FAA, IPAA, 4-OH-A) with LC-MS/MS

mleko / milk (25–250) μg/kg metilaminoantipirin / methylaminoantipyrine (MAA) CCα = 56 μg/kg CCβ = 61 μg/kg ±6,3 μg/kg (25 μg/kg) ±8,2 μg/kg (50 μg/kg) ±15,5 μg/kg (75 μg/kg) antipirin / antipyrine (A) CCα = 55 μg/kg CCβ = 59 μg/kg ±12,2 μg/kg (25 μg/kg) ±8,3 μg/kg (50 μg/kg) ±15,6 μg/kg (75 μg/kg) 4-aminoantipirin / 4-aminoantipyrine (AA) CCα = 54 μg/kg CCβ = 58 μg/kg ±7,8 μg/kg (25 μg/kg) ±6,4 μg/kg (50 μg/kg) ±9,2 μg/kg (75 μg/kg) 4-acetamidoantipirin / 4-acetamidoantipyrine (AAA) CCα = 56 μg/kg CCβ = 61 μg/kg ±6,3 μg/kg (25 μg/kg) ±8,2 μg/kg (50 μg/kg) ±15,5 μg/kg (75 μg/kg) 4-dimetilaminoantipirin / 4-dimethylaminoantipyrine (DMAA) CCα = 53 μg/kg CCβ = 56 μg/kg ±4,7 μg/kg (25 μg/kg) ±5,1 μg/kg (50 μg/kg) ±10,1 μg/kg (75 μg/kg) 4-formilaminoantipirin / 4-formylaminoantipyrine (FAA) CCα = 54 μg/kg CCβ = 59 μg/kg ±12,3 μg/kg (25 μg/kg) ±5,4 μg/kg (50 μg/kg) ±13,9 μg/kg (75 μg/kg) isopropilaminoantipirin / isopropylaminoantipyrine (IPAA) CCα = 52 μg/kg CCβ = 54 μg/kg ±4,3 μg/kg (25 μg/kg) ±3,7 μg/kg (50 μg/kg) ±8,0 μg/kg (75 μg/kg) presejalna metoda / screening method: mleko / milk metilaminoantipirin / methylaminoantipyrine

DA / YES DA / YES





number




Akreditirano* Da/Ne

Accredited Yes/No

(MAA) antipirin / antipyrine (A) 4-aminoantipirin / 4-aminoantipyrine (AA) 4-acetamidoantipirin / 4-acetamidoantipyrine (AAA) 4-dimetilaminoantipirin / 4-dimethylaminoantipyrine (DMAA) 4-formilaminoantipirin / 4-formylaminoantipyrine (FAA) isopropilaminoantipirin / isopropylaminoantipyrine (IPAA) CCβ = 25 μg/kg

46. SOP 316 interna metoda / in-house method

Ugotavljanje ostankov kloramfenikola z GC-ECD Determining chloramphenicol residues by GC-ECD

mleko / milk (0,1–30) μg/kg CCα = 0,18 μg/kg CCβ = 0,21 μg/kg ±0,04 μg/kg (0,3 μg/kg) ±0,08 μg/kg (0,45 μg/kg) ±0,08 μg/kg (0,6 μg/kg) meso / meat (0,1–30) μg/kg CCα = 0,07 μg/kg CCβ = 0,12 μg/kg ±0,04 μg/kg (0,3 μg/kg) ±0,08 μg/kg (0,45 μg/kg) ±0,08 μg/kg (0,6 μg/kg) jajca / eggs (0,1–30) μg/kg CCα = 0,05 μg/kg CCβ = 0,09 μg/kg ±0,04 μg/kg (0,3 μg/kg) ±0,07 μg/kg (0,45 μg/kg) ±0,12 μg/kg (0,6 μg/kg)

NE / NO NE / NO NE / NO

47. SOP 225 interna metoda in-house method

Postopek za določanje vsebnosti zaostankov klorpromazina v vzorcih ledvic, jajc in urina, presejalna metoda HPLC-UV Procedure for the determination residues of chlorpromazine in kidneys, eggs and urin, screening method (HPLC-UV)

jajca / eggs CCβ = 5 g/kg ledvice / kidney CCβ = 5 µg/kg urin / urine CCβ = 5 µg/kg



Ugotavljanje prisotnosti streptomicina z ELISA metodo, presejalna metoda Determining of Streptomycin presence by ELISA method, screening method

med / honey streptomicin / Streptomicin dihidrostreptomicin/Dihydrostreptomicin LOD = 10 μg/kg

NE/ NO


Določanje nizkomolekularnih sladkorjev v živilih s HPTLC

Determination of low molecular weigt carbohidrates in food with HPTLC

živila / food saharoza / saccharose: (0,05–5) g/100 g fruktoza / fructose: (0,05–5) g/100 g maltoza / maltose: (0,05–5) g/100 g laktoza / lactose: (0,05–5) g/100 g glukoza / glucose: (0,05–5) g/100 g

NE / NO

50. IDF 21B: 1987

Določanje vsebnosti skupne suhe snovi v mleku Milk, cream and evaporated milk: Determination of total solid content (reference method) SOP 182, verzija 2

mleko / milk (0–20) g/100g ±0,11 g/100g

NE / NO


Preiskava na tetraciklinske in beta-laktamske antibiotike v mleku s testom TWINSENSOR Screening method for tetracyclines and beta-lactams antibiotics in milk - TWINSENSOR

mleko / milk penicilin G / penicillin G CCβ = 4 µg/kg ampicilin / ampicillin CCβ = 4 µg/kg amoksicilin / amoksicillin CCβ = 4 µg/kg kloksacilin / cloxacillin CCβ = 20 µg/kg nafcilin / nafcillin

NE / NO





number




Akreditirano* Da/Ne

Accredited Yes/No

CCβ = 60 µg/kg cefapirin / cefapirin CCβ = 60 µg/kg cefkvinom / cefquinome CCβ = 20 µg/kg cefalonij / cefalonium CCβ = 20 µg/kg cefoperazon / cefoperazone CCβ = 50 µg/kg ceftiofur / ceftiofur CCβ = 50 µg/kg cefazolin / cefazolin CCβ = 50 µg/kg tetraciklin / tetracycline (TC) CCβ = 100 µg/kg klortetraciklin / chlortetracycline (CTC) CCβ = 100 µg/kg oksitetraciklin / oxytetracycline (OTC) CCβ = 100 µg/kg doksiciklin / doxycycline (DC) CCβ = 30 µg/kg dihidrostreptomicin / dihydrostreptomicine not sensitive gentamicin / gentamycin not sensitive kanamicin / kanamycin not sensitive neomicin / neomycin not sensitive streptomicin / streptomycine very low sensitivity danofloksacin / danofloxacin (DAN) not sensitive enrofloksacin / enrofloxacin (EFC) not sensitive marbofloksacin / marbofloxacin (MAR) not sensitive sulfadiazin / sulfadiazine not sensitive sulfadimidin / sulfadimidine not sensitive sulfadoksin / sulfadoxin not sensitive bacitracin / bacitracin not sensitive linkomicin / lincomycin not sensitive novobiocin / novobiocin not sensitive tilozin / tylosin not sensitive trimetoprim / trimitroprim very low sensitivity


Preiskava na tetraciklinske antibiotike v medu s testom TETRASENSOR Screening method for tetracyclines antibiotics in honey - TETRASENSOR

med / honey tetraciklin / tetracycline (TC) CCβ = 15 µg/kg klortetraciklin / chlortetracycline (CTC) CCβ = 10 µg/kg oksitetraciklin / oxytetracycline (OTC) CCβ = 15 µg/kg doksiciklin / doxycycline (DC) CCβ = 10 µg/kg

NE / NO


Ugotavljanje prisotnosti lipofilnih toksinov v školjkah z biološkim poskusom Determination of lipophilic toxins in shellfish by biological test

školjke / shellfish okadajična kislina/okadaic acid LOD = 160 μg/kg

NE / NO

54. AOAC 959.08 Official Method

Ugotavljanje prisotnosti PSP toksina v školjkah z biološkim poskusom Determination of PSP toxins in shellfish by biological test SOP 313

školjke / shellfish LOQ = 800 μg/kg

NE / NO





number




Akreditirano* Da/Ne

Accredited Yes/No

55. ISO 7932:2005 Določanje števila bakterij Bacillus cereus Horizontal method for the enumeration of presumptive Bacillus cereus

meso in mesni izdelki / meat and meat products LOD = 10 CFU/g (ml) ±0,26 log10 CFU/g mleko in mlečni izdelki / milk and milk products LOD = 10 CFU/g (ml) ±0,26 log10 CFU/g

NE / NO

56. ISO 10273:2003 Modifikacija / modification

Ugotavljanje prisotnosti bakterije Yersinia enterocolitica Horizontal method for the detection Yersinia enterocolitica

meso in mesni izdelki / meat and meat products mleko in mlečni izdelki / milk and milk products brisi okolja / environmental swabs

NE / NO

57. ISO 15213 Ugotavljanje prisotnosti in števila sulfit reducirajoči klostridijev, ki rastejo v anaerobnih pogojih Horizontal method for the enumeration of sulfite-reducing bacteria growing under anaerobic conditions

Živila, voda / Food, water NE / NO

58. ISO 15216-2 Ugotavljanje prisotnosti virusa hepatitisa A z uporabo PCR v realnem času Kvalitativna metoda Horizontal method for determination of hepatitis A virus using real-time RT-PCR Part 2: Method for detection

Živila (školjke) / Food (shellfish) NE / NO

59. ISO 15216-2 Ugotavljanje prisotnosti norovirusov z uporabo PCR v realnem času Kvalitativna metoda Horizontal method for determination of norovirus using real-time RT-PCR Part 2: Method for detection

Živila (školjke) / Food (shellfish) NE / NO

60. ISO 21807 Določanje aktivnosti vode Determination of water activity

Živila / Food NE / NO

61. ISO 21872-1 Ugotavljanje prisotnosti bakterije Vibrio parahaemolyticus Horizontal method for detection of Vibrio parahaemolyticus


62. Evropska izločilna metoda EU-RL za koagulaza pozitivne stafilokoke European screening method of the EU-RL for CPS

Ugotavljanje prisotnosti stafilokoknih enterotoksinov tipa A-E Detection of staphylococcal enterotoxins types SEA-SEE


63. interna metoda in-house method

Ugotavljanje prisotnosti bakterije Clostridium difficile z uporabo PCR v realnem času Horizontal method for detection of Clostridium difficile using real-time RT-PCR

Meso in mesni izdelki / Meat and meat products LOD ≥ 5 CFU

NE / NO


Ugotavljanje prisotnosti bakterije Clostridium difficile – izolacija Horizontal method for detection of Clostridium difficile - isolation

Meso in mesni izdelki / Meat and meat products LOD ≥ 5 CFU

NE / NO

65. ISO 17410 Ugotavljanje števila psihrotropnih mikroorganizmov Horizontal method for the enumeration of psychrotropic microorganisms

Živila / Food LOD ≥ 10 CFU

NE / NO

66. ISO 15214 Ugotavljanje števila mezofilnih mlečnokislinskih bakterij – štetje na ploščah pri 30 °C Horizontal method for the enumeration of mesophilic lactic acid bacteria – Colony-count technique at 30 °C


NE / NO


Ugotavljanje števila plesni in kvasovk Horizontal method for the enumeration of molds and yeasts


NE / NO


Ugotavljanje števila anaerobnih bakterij - štetje na ploščah pri 30 °C Horizontal method for the enumeration of anaerobic bacteria – Colony-count technique at 30 °C


NE / NO


Ugotavljanje števila sporogenih bakterij - štetje na ploščah pri 30 °C Horizontal method for the enumeration of sporogenic bacteria – Colony-count technique at 30 °C


NE / NO





number




Akreditirano* Da/Ne

Accredited Yes/No


Ugotavljanje prisotnosti tkiv centralnega živčnega sistema - ELISA Detection of central nervous system tissue - ELISA

Brisi / Swabs NE / NO

Inštitut za varno hrano, krmo in okolje; Enota za okolje, prehrano, dobrobit in higieno živali

Št. No.

Oznaka preskusne metode Internal/standard reference number



Material, products; Measurement uncertainty(expressed as expanded measurement uncertainty U); Method limits

Akreditirano*

Da/Ne Accredited Yes/No

71. SOP 196, verzija 4 Interna metoda / In-house method

Določanje aflatoksina M1 v mleku in mlečnih izdelkih; HPLC-fluorescenčna detekcija Determination of aflatoxin M1 in milk and dairy products; HPLC-fluorescence

Mleko / Milk 15–1000 ng/kg LOD = 5 ng/kg LOQ = 15 ng/kg 5,5 ng/kg (25 ng/kg) 6,8 ng/kg (50 ng/kg) 8,9 ng/kg (75 ng/kg) Sir, sladoled, smetana, jogurt / Cheese, ice-cream, cream, yoghurt 45–500 ng/kg LOD = 15 ng/kg LOQ = 45 ng/kg 11 ng/kg (50 ng/kg )

DA / YES DA / YES


Določanje aflatoksina B1 v živilih živalskega izvora; HPLC-fluorescenčna detekcija Determination of aflatoxin B1 in food of animal origin; HPLC-fluorescence

Jetra / Liver 15–75 ng/kg LOD = 5 ng/kg LOQ = 15 ng/kg 7 ng/kg (25 ng/kg) 16 ng/kg (50 ng/kg) 23 ng/kg (75 ng/kg) Jajca / Eggs 6–50 ng/kg LOD = 2 ng/kg LOQ = 6 ng/kg 10 ng/kg (50 ng/kg )

DA / YES DA / YES


Določanje ohratoksina A v živilih živalskega izvora; HPLC-fluorescenčna detekcija Determination of ochratoxin A in food of animal origin; HPLC-fluorescence

Ledvice / Kidneys 2,5–7,5 g/kg LOD = 0,8 g/kg LOQ = 2,5 g/kg 0,21 g/kg (2,5 g/kg) 0,51 g/kg (5,0 g/kg) 0,62 g/kg (7,5 g/kg)

DA / YES


Določanje aflatoksinov v krmi in sestavinah krme; HPLC-fluorescenčna detekcija Determination of aflatoxins in feed and feed ingredients; HPLC-fluorescence

Krma / Feed Aflatoksin B1 / Aflatoxin B1

0,0006–0,030 mg/kg LOD = 0,0002 mg/kg LOQ = 0,0006 mg/kg 30 % g/kg (0,0006–0,030 mg/kg) Aflatoksin B2, G1, G2 / Aflatoxin B2,G1,G2 0,001–0,010 mg/kg LOD = 0,0004 mg/kg LOQ = 0,001 mg/kg 30 % (0,001–0,010 mg/kg)

DA / YES Da / Yes Ne / No


Določanje ohratoksina A v krmi in sestavinah krme; HPLC-fluorescenčna detekcija Determination of ochratoxin A in feed and feed ingredients; HPLC-fluorescence

Krma / Feed 0,03–0,3 mg/kg LOD = 0,01 mg/kg LOQ = 0,03 mg/kg 0,023 mg/kg (0,1 mg/kg) 0,047 mg/kg (0,2 mg/kg) 0,072 mg/kg (0,3 mg/kg)

DA / YES


Določanje zearalenona v krmi in sestavinah krme; HPLC-fluorescenčna detekcija Determination of zearalenone in feed and feed ingredients; HPLC-fluorescence

Krma / Feed 0,05–0,3 mg/kg LOD = 0,02 mg/kg LOQ = 0,05 mg/kg 0,017 mg/kg (0,1 mg/kg) 0,037 mg/kg (0,2 mg/kg) 0,052 mg/kg (0,3 mg/kg)

DA / YES




Št. No.





Akreditirano*


77. ISO 14183:2005

Določanje monenzina, narazina and salinomicina v krmi, premiksih in koncentratih; HPLC-UV detekcija s pokolonsko derivatizacijo Determination of monensin, narasin and salinomycin in feed, premixes and concentrates; HPLC- UV detection with post-column derivatization SOP 272, verzija 4

Krma / Feed Premiksi / Premixes Koncentrati / Concentrates Monenzin / Monensin 1 mg/kg–180 g/kg LOD = 0,3 mg/kg LOQ = 1 mg/kg 0,1 mg/kg (0,9 mg/kg) 3,1 mg/kg (90 mg/kg) 2,3 g/kg (18 g/kg) 1,7 % (180 g) Salinomicin / Salinomycin 2 mg/kg–120 g/kg LOD = 0,5 mg/kg LOQ = 2 mg/kg 0,2 mg/kg (1,7 mg/kg) 4,6 mg/kg (63 mg/kg) 1,7 g/kg (12 g/kg) 1,2 % (120 g) Krma / Feed Narazin / Narasin LOD = 0,5 mg/kg LOQ = 2 mg/kg 0,31 mg/kg (2 mg/kg)

DA / YES DA / YES DA / YES Da /Yes Da /Yes DA / YES Da /Yes


Določanje semduramicina v krmi, premiksih in koncentratih; HPLC-UV/VIS detekcija s pokolonsko derivatizacijo Determination of semduramicin in feed, premixes and concentrates; HPLC- UV/VIS detection with post-column derivatization

Premiksi, koncentrati / Premixes, concentrates 0,1–50 g/kg LOD = 0,04 g/kg LOQ = 0,1 g/kg 0,2 g/kg (2,5 g/kg) 0,7 % (50 g)

DA / YES


Določanje fumonizinov B1 in B2 v krmi in sestavinah krme; HPLC-fluorescenčna detekcija Determination of fumonisins B1 and B2 in feed and feed ingredients; HPLC-fluorescence

Koruza, krma na osnovi koruze / Maize, maize based feed Fumonizin B1 / Fumonisin B1 0,3–1,7 mg/kg LOD = 0,1 mg/kg LOQ = 0,3 mg/kg 25 % (0,3–1,7 mg/kg) Fumonizin B2 / Fumonisin B2 0,3–0,5 mg/kg LOD = 0,1 mg/kg LOQ = 0,3 mg/kg 25 % (0,3–0,5 mg/kg)

DA / YES Da /Yes Da /Yes

80. Uredba Komisije (ES) št. 152/2009, Priloga III, Metoda A Commission Regulation (EC) No 152/2009, Annex III, Method A

Določanje vlage s tehtanjem Determination of moisture by gravimetry SOP 250, verzija 4

Krmne mešanice, sestavine krme, premiksi, dodatki krmi / Compound feed, feed material, premixtures, feed additives 6,50–83 %

DA / YES


Določanje trihotecenov tipa A in B v krmi in sestavinah krme; GC-MS po derivatizaciji Determination of type A and type B trichothecenes in feed and feed ingredients; GC-MS after derivatization

Krma / Feed 0,1–1,0 mg/kg LOD = 0,05 mg/kg LOQ = 0,1 mg/kg Deoksinivalenol / Deoxynivalenol (DON) 0,09 mg/kg (0,3 mg/kg) 0,17 mg/kg (0,5 mg/kg) 0,33 mg/kg (1,0 mg/kg) 3-Acetildeoksinivalenol / 3-Acetyldeoxynivalenol (3-AcDON) 0,08 mg/kg (0,3 mg/kg) 0,16 mg/kg (0,5 mg/kg) 0,35 mg/kg (1,0 mg/kg) Diacetoksiscirpenol / Diacetoxyscirpenol (DAS) 0,07 mg/kg (0,3 mg/kg) 0,12 mg/kg (0,5 mg/kg) 0,23 mg/kg (1,0 mg/kg)

DA / YES




Št. No.





Akreditirano*


15-Acetildeoksinivalenol / 15-Acetyldeoxynivalenol (15-AcDON) 0,07 mg/kg (0,3 mg/kg) 0,15 mg/kg (0,5 mg/kg) 0,25 mg/kg (1,0 mg/kg) Nivalenol / Nivalenol (NIV) 0,08 mg/kg (0,3 mg/kg) 0,18 mg/kg (0,5 mg/kg) 0,27 mg/kg (1,0 mg/kg) Neosolaniol / Neosolaniol (NSOL) 0,07 mg/kg (0,3 mg/kg) 0,13 mg/kg (0,5 mg/kg) 0,24 mg/kg (1,0 mg/kg) HT-2 0,07 mg/kg (0,3 mg/kg) 0,18 mg/kg (0,5 mg/kg) 0,26 mg/kg (1,0 mg/kg) T-2 0,07 mg/kg (0,3 mg/kg) 0,14 mg/kg (0,5 mg/kg) 0,25 mg/kg (1,0 mg/kg)


Določanje elementov v krmi; ICP - MS Determination of elements in feed; ICP-MS

Krma / Feed Mn 3,539500 mg/kg LOD = 1,06 mg/kg LOQ = 3,53 mg/kg 7,60 mg/kg (65,4 mg/kg) 7,62 mg/kg (71,7 mg/kg) Fe 6,625800 mg/kg LOD = 1,98 mg/kg LOQ = 6,62 mg/kg 20,4 mg/kg (180 mg/kg) 21,0 mg/kg (278 mg/kg) 162 mg/kg (957 mg/kg) Co 0,00819 mg/kg LOD = 0,002 mg/kg LOQ = 0,008 mg/kg 0,028 mg/kg (0,216 mg/kg) 0,029 mg/kg (0,355 mg/kg) Cu 0,651200 mg/kg LOD = 0,19 mg/kg LOQ = 0,65 mg/kg 0,79 mg/kg (7,24 mg/kg) 1,37 mg/kg (9,8 mg/kg) 1,23 mg/kg (10,3 mg/kg) Zn 1,057200 mg/kg LOD = 0,31 mg/kg LOQ = 1,05 mg/kg 2,22 mg/kg (24,4 mg/kg) 2,63 mg/kg (26,7 mg/kg) 4,45 mg/kg (66,7 mg/kg) Mo 0,0047 mg/kg LOD = 0,001 mg/kg LOQ = 0,004 mg/kg 0,59 mg/kg (0,78 mg/kg) 0,70 mg/kg (1,85 mg/kg) 0,34 mg/kg (2,36 mg/kg) Cd 0,0052,89 mg/kg LOD = 0,002 mg/kg LOQ = 0,005 mg/kg

DA / YES




Št. No.





Akreditirano*


0,004 mg/kg (0,104 mg/kg) 0,30 mg/kg (1,78 mg/kg) 0,11 mg/kg (2,58 mg/kg) Hg 0,0251,88 mg/kg LOD = 0,008 mg/kg LOQ = 0,025 mg/kg 0,007 mg/kg (0,023 mg/kg) 0,38 mg/kg (1,88 mg/kg) Pb 0,0155,65 mg/kg LOD = 0,005 mg/kg LOQ = 0,015 mg/kg 0,03 mg/kg (0,19 mg/kg) 0,14 mg/kg (1,67 mg/kg) 0,31 mg/kg (1,81 mg/kg) As 0,0262,82 mg/kg LOD = 0,008 mg/kg LOQ = 0,026 mg/kg 0,010 mg/kg (0,044 mg/kg) 0,017 mg/kg (0,076 mg/kg) 0,40 mg/kg (1,94 mg/kg) Se 0,03344 mg/kg LOD = 0,010 mg/kg LOQ = 0,033 mg/kg 0,012 mg/kg (0,032 mg/kg) 0,02 mg/kg (0,12 mg/kg) 0,28 mg/kg (1,94 mg/kg)


Določanje kokcidiostatikov v krmi za neciljne živali; LC-MS/MS Determination of coccidiostats in non-target feed; LC-MS/MS

Krma / Feed Natrijev lasalocid / Lasalocid sodium 0,12512,5 mg/kg LOD = 0,04 mg/kg LOQ = 0,125 mg/kg 0,29 mg/kg (1,25 mg/kg) 0,31 mg/kg (3,75 mg/kg) Narazin / Narasin 0,077,0 mg/kg LOD = 0,02 mg/kg LOQ = 0,07 mg/kg 0,18 mg/kg (0,7 mg/kg) 0,37 mg/kg (2,1 mg/kg) Natrijev salinomicin / Salinomycin sodium 0,077,0 mg/kg LOD = 0,02 mg/kg LOQ = 0,07 mg/kg 0,14 mg/kg (0,7 mg/kg) 0,38 mg/kg (2,1 mg/kg) Natrijev monenzin / Monensin sodium 0,125–12,5 mg/kg LOD = 0,04 mg/kg LOQ = 0,125 mg/kg 0,26 mg/kg (1,25 mg/kg) 0,76 mg/kg (3,75 mg/kg) Natrijev semduramicin / Semduramicin sodium 0,025–2,5 mg/kg LOD = 0,01 mg/kg LOQ = 0,025 mg/kg 0,11 mg/kg (0,25 mg/kg) 0,27 mg/kg (0,75 mg/kg) Amonijev maduramicin / Maduramicin ammonium 0,005–0,5 mg/kg LOD = 0,002 mg/kg LOQ = 0,005 mg/kg 0,022 mg/kg (0,05 mg/kg) 0,054 mg/kg (0,15 mg/kg)

DA / YES




Št. No.





Akreditirano*


Robenidin hidroklorid / Robenidine hydrochloride 0,07–7,0 mg/kg LOD = 0,02 mg/kg LOQ = 0,07 mg/kg 0,18 mg/kg (0,7 mg/kg) 0,42 mg/kg (2,1 mg/kg) Dekokvinat / Decoquinate 0,04–1,77 mg/kg LOD = 0,01 mg/kg LOQ = 0,04 mg/kg 0,15 mg/kg (0,4 mg/kg) 0,43 mg/kg (1,2 mg/kg) Halofuginon hidrobromid / Halofuginone hydrobromide 0,003–0,3 mg/kg LOD = 0,001 mg/kg LOQ = 0,003 mg/kg 0,006 mg/kg (0,03 mg/kg) 0,016 mg/kg (0,09 mg/kg) Nikarbazin / Nicarbazin 0,05–12,5 mg/kg LOD = 0,02 mg/kg LOQ = 0,05 mg/kg 0,12 mg/kg (1,25 mg/kg) 0,20 mg/kg (3,75 mg/kg) Diklazuril / Diclazuril 0,005–0,1 mg/kg LOD = 0,002 mg/kg LOQ = 0,005 mg/kg 0,003 mg/kg (0,01 mg/kg) 0,006 mg/kg (0,03 mg/kg)


Določanje beta-agonistov v živilih, krmi in bioloških vzorcih; LC-MS/MS Determination of beta-agonists; in food, feed and biological samples; LC-MS/MS

Jetra / Liver Potrditvena metoda / Confirmatory method Brombuterol / Brombuterol 0,1–1 µg/kg CCα = 0,26 μg/kg CCβ = 0,31 μg/kg ±0,02 μg/kg (0,1 μg/kg) ±0,07 μg/kg (0,2 μg/kg) ±0,11 μg/kg (0,3 μg/kg) Bromklorbuterol / Bromchlorbuterol 0,1–1 µg/kg CCα = 0,24 μg/kg CCβ = 0,27 μg/kg ±0,02 μg/kg (0,1 μg/kg) ±0,04 μg/kg (0,2 μg/kg) ±0,09 μg/kg (0,3 μg/kg) Cimaterol / Cimaterol 0,25–2,5 µg/kg CCα = 0,60 μg/kg CCβ = 0,70 μg/kg ±0,08 μg/kg (0,25 μg/kg) ±0,12 μg/kg (0,5 μg/kg) ±0,19 μg/kg (0,75 μg/kg) Cimbuterol / Cimbuterol 0,25–2,5 µg/kg CCα = 0,59 μg/kg CCβ = 0,68 μg/kg ±0,14 μg/kg (0,25 μg/kg) ±0,11 μg/kg (0,5 μg/kg) ±0,14 μg/kg (0,75 μg/kg) Klenbuterol / Clenbuterol 0,05–0,5 µg/kg CCα = 0,12 μg/kg CCβ = 0,13 μg/kg ±0,02 μg/kg (0,05 μg/kg) ±0,02 μg/kg (0,1 μg/kg) ±0,04 μg/kg (0,15 μg/kg)

DA / YES DA / YES




Št. No.





Akreditirano*


Klenciklohekserol / Clencyclohexerol 0,5–5 µg/kg CCα = 1,53 μg/kg CCβ = 2,06 μg/kg ±0,57 μg/kg (0,5 μg/kg) ±0,65 μg/kg (1,0 μg/kg) ±0,98 μg/kg (1,5 μg/kg) Klenhekserol / Clenhexerol 0,5–5 µg/kg CCα = 1,40 μg/kg CCβ = 1,79 μg/kg ±0,33 μg/kg (0,5 μg/kg) ±0,48 μg/kg (1,0 μg/kg) ±0,84 μg/kg (1,5 μg/kg) Klenpenterol / Clenpenterol 0,25–2,5 µg/kg CCα = 0,72 μg/kg CCβ = 0,94 μg/kg ±0,13 μg/kg (0,25 μg/kg) ±0,27 μg/kg (0,5 μg/kg) ±0,46 μg/kg (0,75 μg/kg) Klenproperol / Clenproperol 0,25–2,5 µg/kg CCα = 0,61 µg/kg CCβ = 0,73 µg/kg ±0,04 μg/kg (0,25 μg/kg) ±0,14 μg/kg (0,5 μg/kg) ±0,31 μg/kg (0,75 μg/kg) Fenoterol / Fenoterol 0,5–5 µg/kg CCα = 1,23 µg/kg CCβ = 1,47 µg/kg ±0,24 μg/kg (0,5 μg/kg) ±0,28 μg/kg (1,0 μg/kg) ±0,33 μg/kg (1,5 μg/kg) Izokssuprin / Isoxsuprine 0,25–2,5 µg/kg CCα = 0,65 µg/kg CCβ = 0,79 µg/kg ±0,08 μg/kg (0,25 μg/kg) ±0,18 μg/kg (0,5 μg/kg) ±0,16 μg/kg (0,75 μg/kg) Mabuterol / Mabuterol 0,1–1 µg/kg CCα = 0,33 µg/kg CCβ = 0,46 µg/kg ±0,07 μg/kg (0,1 μg/kg) ±0,16 μg/kg (0,2 μg/kg) ±0,17 μg/kg (0,3 μg/kg) Mapenterol / Mapenterol 0,1–1 µg/kg CCα = 0,27 µg/kg CCβ = 0,34 µg/kg ±0,05 μg/kg (0,1 μg/kg) ±0,09 μg/kg (0,2 μg/kg) ±0,14 μg/kg (0,3 μg/kg) Raktopamin / Ractopamine 0,25–2,5 µg/kg CCα = 0,61 µg/kg CCβ = 0,72µg/kg ±0,10 μg/kg (0,25 μg/kg) ±0,13 μg/kg (0,5 μg/kg) ±0,14 μg/kg (0,75 μg/kg) Ritodrin / Ritodrine 0,25–2,5 µg/kg CCα = 0,69 μg/kg CCβ = 0,89 μg/kg ±0,09 μg/kg (0,25 μg/kg) ±0,24 μg/kg (0,5 μg/kg) ±0,15 μg/kg (0,75 μg/kg)




Št. No.





Akreditirano*


Salbutamol / Salbutamol 0,5–5 µg/kg CCα = 1,07 µg/kg CCβ = 1,14 µg/kg ±0,07 μg/kg (0,5 μg/kg) ±0,09 μg/kg (1,0 μg/kg) ±0,35 μg/kg (1,5 μg/kg) Salmeterol / Salmeterol 0,5–5 µg/kg CCα = 1,28µg/kg CCβ = 1,56 µg/kg ±0,21 μg/kg (0,5 μg/kg) ±0,34 μg/kg (1,0 μg/kg) ±0,27 μg/kg (1,5 μg/kg) Terbutalin / Terbutaline 0,5–5 µg/kg CCα = 1,19 µg/kg CCβ = 1,37 µg/kg ±0,14 μg/kg (0,5 μg/kg) ±0,23 μg/kg (1,0 μg /kg) ±0,23 μg/kg (1,5 μg /kg) Tulobuterol / Tulobuterol 0,1–1 µg/kg CCα = 0,31 µg/kg CCβ = 0,42 µg/kg ±0,03 μg/kg (0,1 μg/kg) ±0,13 μg/kg (0,2 μg/kg) ±0,14 μg/kg (0,3 μg/kg) Zilpaterol / Zilpaterol 0,5–5 µg/kg CCα = 1,21 µg/kg CCβ = 1,42 µg/kg ±0,25 μg/kg (0,5 μg/kg) ±0,26 μg/kg (1,0 μg/kg) ±0,80 μg/kg (1,5 μg/kg) Presejalna metoda / Screening method Brombuterol / Brombuterol CCβ = 0,10 µg/kg Bromklorbuterol / Bromchlorbuterol CCβ = 0,10 µg/kg Cimaterol / Cimaterol CCβ = 0,25 µg/kg Cimbuterol / Cimbuterol CCβ = 0,25 µg/kg Klenbuterol / Clenbuterol CCβ = 0,05 µg/kg Klenciklohekserol / Clencyclohexerol CCβ = 0,50 µg/kg Klenhekserol / Clenhexerol CCβ = 0,50 μg/kg Klenpenterol / Clenpenterol CCβ = 0,25 µg/kg Klenproperol / Clenproperol CCβ = 0,25 µg/kg Fenoterol / Fenoterol CCβ = 0,50 µg/kg Izokssuprin / Isoxsuprine CCβ = 0,25 µg/kg Mabuterol / Mabuterol CCβ = 0,10 µg/kg Mapenterol / Mapenterol CCβ = 0,10 µg/kg Raktopamin / Ractopamine CCβ = 0,25 µg/kg Ritodrin / Ritodrine CCβ = 0,25 µg/kg Salbutamol / Salbutamol CCβ = 0,50 µg/kg Salmeterol / Salmeterol CCβ = 0,50 µg/kg Terbutalin / Terbutaline CCβ = 0,50 µg/kg Tulobuterol / Tulobuterol CCβ = 0,10 µg/kg Zilpaterol / Zilpaterol CCβ = 0,50 µg/kg

DA / YES




Št. No.





Akreditirano*


Urin / Urine Potrditvena metoda / Confirmatory method Brombuterol / Brombuterol (0,1–1) µg/kg CCα = 0,23 μg/kg CCβ = 0,25 μg/kg ±0,02 μg/kg (0,1 μg /kg) ±0,03 μg/kg (0,2 μg /kg) ±0,04 μg/kg (0,3 μg /kg) Bromklorbuterol / Bromchlorbuterol (0,1–1) µg/kg CCα = 0,23 μg/kg CCβ = 0,26 μg/kg ±0,02 μg/kg (0,1 μg /kg) ±0,03 μg/kg (0,2 μg /kg) ±0,03 μg/kg (0,3 μg /kg) Cimaterol / Cimaterol (0,25–2,5) µg/kg CCα = 0,61 μg/kg CCβ = 0,71 μg/kg ±0,08 μg/kg (0,25 μg /kg) ±0,13 μg/kg (0,5 μg /kg) ±0,19 μg/kg (0,75 μg /kg) Cimbuterol / Cimbuterol (0,25–2,5) µg/kg CCα = 0,59 μg/kg CCβ = 0,67 μg/kg ±0,06 μg/kg (0,25 μg /kg) ±0,11 μg/kg (0,5 μg /kg) ±0,19 μg/kg (0,75 μg /kg) Klenbuterol / Clenbuterol (0,05–0,5) µg/kg CCα = 0,11 μg/kg CCβ = 0,12 μg/kg ±0,01 μg/kg (0,05 μg /kg) ±0,01 μg/kg (0,1 μg /kg) ±0,02 μg/kg (0,15 μg /kg) Klenciklohekserol / Clencyclohexerol (0,5–5,0) µg/kg CCα = 1,23 μg/kg CCβ = 1,45 μg/kg ±0,24 μg/kg (0,5 μg /kg) ±0,28 μg/kg (1,0 μg /kg) ±0,51 μg/kg (1,5 μg /kg) Klenhekserol / Clenhexerol (0,5–5) µg/kg CCα = 1,51 μg/kg CCβ = 2,02 μg/kg ±0,23 μg/kg (0,5 μg /kg) ±0,62 μg/kg (1,0 μg /kg) ±0,55 μg/kg (1,5 μg /kg) Klenpenterol / Clenpenterol (0,25–2,5) µg/kg CCα = 0,69 μg/kg CCβ = 0,89 μg/kg ±0,14 μg/kg (0,25 μg /kg) ±0,24 μg/kg (0,5 μg /kg) ±0,61 μg/kg (0,75 μg /kg) Klenproperol / Clenproperol (0,25–2,5) µg/kg CCα = 0,59 µg/kg CCβ = 0,69 µg/kg ±0,09 μg/kg (0,25 μg /kg) ±0,11 μg/kg (0,5 μg /kg) ±0,13 μg/kg (0,75 μg /kg) Izokssuprin / Isoxsuprine (0,25–2,5) µg/kg CCα = 0,62 µg/kg CCβ = 0,74 µg/kg ±0,09 μg/kg (0,25 μg /kg) ±0,14 μg/kg (0,5 μg /kg)

DA / YES DA / YES




Št. No.





Akreditirano*


±0,19 μg/kg (0,75 μg /kg) Mabuterol / Mabuterol (0,1–1) µg/kg CCα = 0,25 µg/kg CCβ = 0,30 µg/kg ±0,08 μg/kg (0,1 μg /kg) ±0,06 μg/kg (0,2 μg /kg) ±0,38 μg/kg (0,3 μg /kg) Mapenterol / Mapenterol (0,1–1) µg/kg CCα = 0,23 µg/kg CCβ = 0,26 µg/kg ±0,04 μg/kg (0,1 μg /kg) ±0,04 μg/kg (0,2 μg /kg) ±0,08 μg/kg (0,3 μg /kg) Raktopamin / Ractopamine (0,25–2,5) µg/kg CCα = 0,55 µg/kg CCβ = 0,61µg/kg ±0,04 μg/kg (0,25 μg /kg) ±0,07 μg/kg (0,5 μg /kg) ±0,24 μg/kg (0,75 μg /kg) Ritodrin / Ritodrine (0,25–2,5) µg/kg CCα = 0,57 μg/kg CCβ = 0,65 μg/kg ±0,05 μg/kg (0,25 μg /kg) ±0,09 μg/kg (0,5 μg /kg) ±0,21 μg/kg (0,75 μg /kg) Salbutamol / Salbutamol (0,5–5) µg/kg CCα = 1,07 µg/kg CCβ = 1,14 µg/kg ±0,11 μg/kg (0,5 μg /kg) ±0,09 μg/kg (1,0 μg /kg) ±0,23 μg/kg (1,5 μg /kg) Salmeterol / Salmeterol (0,5–5) µg/kg CCα = 1,17µg/kg CCβ = 1,34 µg/kg ±0,09 μg/kg (0,5 μg /kg) ±0,21 μg/kg (1,0 μg /kg) ±0,27 μg/kg (1,5 μg /kg) Terbutalin / Terbutaline (0,5–5) µg/kg CCα = 1,48 µg/kg CCβ = 1,97 µg/kg ±0,10 μg/kg (0,5 μg /kg) ±0,59 μg/kg (1,0 μg /kg) ±1,29 μg/kg (1,5 μg /kg) Tulobuterol / Tulobuterol (0,1–1) µg/kg CCα = 0,32 µg/kg CCβ = 0,43 µg/kg ±0,08 μg/kg (0,1 μg /kg) ±0,14 μg/kg (0,2 μg /kg) ±0,13 μg/kg (0,3 μg /kg) Zilpaterol / Zilpaterol (0,5–5) µg/kg CCα = 1,31 µg/kg CCβ = 1,61 µg/kg ±0,12 μg/kg (0,5 μg /kg) ±0,37 μg/kg (1,0 μg /kg) ±0,71 μg/kg (1,5 μg /kg) Presejalna metoda / Screening method Brombuterol / Brombuterol CCβ = 0,10 µg/kg Bromklorbuterol / Bromchlorbuterol CCβ = 0,10 µg/kg Cimaterol / Cimaterol CCβ = 0,25 µg/kg

DA / YES




Št. No.





Akreditirano*


Cimbuterol / Cimbuterol CCβ = 0,25 µg/kg Klenbuterol / Clenbuterol CCβ = 0,05 µg/kg Klenciklohekserol / Clencyclohexerol CCβ = 0,50 µg/kg Klenhekserol / Clenhexerol CCβ = 0,50 μg/kg Klenpenterol / Clenpenterol CCβ = 0,25 µg/kg Klenproperol / Clenproperol CCβ = 0,25 µg/kg Izokssuprin / Isoxsuprine CCβ = 0,25 µg/kg Mabuterol / Mabuterol CCβ = 0,10 µg/kg Mapenterol / Mapenterol CCβ = 0,10 µg/kg Raktopamin / Ractopamine CCβ = 0,25 µg/kg Ritodrin / Ritodrine CCβ = 0,25 µg/kg Salbutamol / Salbutamol CCβ = 0,50 µg/kg Salmeterol / Salmeterol CCβ = 0,50 µg/kg Terbutalin / Terbutaline CCβ = 0,50 µg/kg Tulobuterol / Tulobuterol CCβ = 0,10 µg/kg Zilpaterol / Zilpaterol CCβ = 0,50 µg/kg Mleko / Milk Potrditvena metoda / Confirmatory method Brombuterol / Brombuterol 0,1–1 µg/kg CCα = 0,26 μg/kg CCβ = 0,31 μg/kg ±0,02 μg/kg (0,1 μg/kg) ±0,07 μg/kg (0,2 μg/kg) ±0,11 μg/kg (0,3 μg/kg) Bromklorbuterol / Bromchlorbuterol 0,1–1 µg/kg CCα = 0,24 μg/kg CCβ = 0,27 μg/kg ±0,02 μg/kg (0,1 μg/kg) ±0,04 μg/kg (0,2 μg/kg) ±0,09 μg/kg (0,3 μg/kg) Cimaterol / Cimaterol 0,25–2,5 µg/kg CCα = 0,60 μg/kg CCβ = 0,70 μg/kg ±0,08 μg/kg (0,25 μg/kg) ±0,12 μg/kg (0,5 μg/kg) ±0,19 μg/kg (0,75 μg/kg) Cimbuterol / Cimbuterol 0,1–1 µg/kg CCα = 0,59 μg/kg CCβ = 0,68 μg/kg ±0,14 μg/kg (0,25 μg/kg) ±0,11 μg/kg (0,5 μg/kg) ±0,14 μg/kg (0,75 μg/kg) Klenbuterol / Clenbuterol 0,05–0,5 µg/kg CCα = 0,06 μg/kg CCβ = 0,08 μg/kg ±0,02 μg/kg (0,05 μg/kg) ±0,02 μg/kg (0,1 μg/kg) ±0,04 μg/kg (0,15 μg/kg) Klenciklohekserol / Clencyclohexerol 0,5–5 µg/kg CCα = 1,53 μg/kg CCβ = 2,06 μg/kg ±0,57 μg/kg (0,5 μg/kg) ±0,65 μg/kg (1,0 μg/kg)

DA / YES DA / YES




Št. No.





Akreditirano*


±0,98 μg/kg (1,5 μg/kg) Klenhekserol / Clenhexerol 0,5–5 µg/kg CCα = 1,40 μg/kg CCβ = 1,79 μg/kg ±0,33 μg/kg (0,5 μg/kg) ±0,48 μg/kg (1,0 μg/kg) ±0,84 μg/kg (1,5 μg/kg) Klenpenterol / Clenpenterol 0,25–2,5 µg/kg CCα = 0,72 μg/kg CCβ = 0,94 μg/kg ±0,13 μg/kg (0,25 μg/kg) ±0,27 μg/kg (0,5 μg/kg) ±0,46 μg/kg (0,75 μg/kg) Klenproperol / Clenproperol 0,25–2,5 µg/kg CCα = 0,61 µg/kg CCβ = 0,73 µg/kg ±0,04 μg/kg (0,25 μg/kg) ±0,14 μg/kg (0,5 μg/kg) ±0,31 μg/kg (0,75 μg/kg) Fenoterol / Fenoterol 0,5–5 µg/kg CCα = 1,23 µg/kg CCβ = 1,47 µg/kg ±0,24 μg/kg (0,5 μg/kg) ±0,28 μg/kg (1,0 μg/kg) ±0,33 μg/kg (1,5 μg/kg) Izokssuprin / Isoxsuprine 0,25–2,5 µg/kg CCα = 0,65 µg/kg CCβ = 0,79 µg/kg ±0,08 μg/kg (0,25 μg/kg) ±0,18 μg/kg (0,5 μg/kg) ±0,16 μg/kg (0,75 μg/kg) Mabuterol / Mabuterol 0,1–1 µg/kg CCα = 0,33 µg/kg CCβ = 0,46 µg/kg ±0,07 μg/kg (0,1 μg/kg) ±0,16 μg/kg (0,2 μg/kg) ±0,17 μg/kg (0,3 μg/kg) Mapenterol / Mapenterol 0,1–1 µg/kg CCα = 0,27 µg/kg CCβ = 0,34 µg/kg ±0,05 μg/kg (0,1 μg/kg) ±0,09 μg/kg (0,2 μg/kg) ±0,14 μg/kg (0,3 μg/kg) Raktopamin / Ractopamine 0,25–2,5 µg/kg CCα = 0,61 µg/kg CCβ = 0,72 µg/kg ±0,10 μg/kg (0,25 μg /kg) ±0,13 μg/kg (0,5 μg /kg) ±0,14 μg/kg (0,75 μg /kg) Ritodrin / Ritodrine 0,25–2,5 µg/kg CCα = 0,69 μg/kg CCβ = 0,89 μg/kg ±0,09 μg/kg (0,25 μg/kg) ±0,24 μg/kg (0,5 μg/kg) ±0,15 μg/kg (0,75 μg/kg) Salbutamol / Salbutamol 0,5–5 µg/kg CCα = 1,07 µg/kg CCβ = 1,14 µg/kg ±0,07 μg/kg (0,5 μg/kg) ±0,09 μg/kg (1,0 μg/kg) ±0,35 μg/kg (1,5 μg/kg)




Št. No.





Akreditirano*


Salmeterol / Salmeterol 0,5–5 µg/kg CCα = 1,28µg/kg CCβ = 1,56 µg/kg ±0,21 μg/kg (0,5 μg/kg) ±0,34 μg/kg (1,0 μg/kg) ±0,27 μg/kg (1,5 μg/kg) Terbutalin / Terbutaline 0,5–5 µg/kg CCα = 1,19 µg/kg CCβ = 1,37 µg/kg ±0,14 μg/kg (0,5 μg/kg) ±0,23 μg/kg (1,0 μg/kg) ±0,23 μg/kg (1,5 μg/kg) Tulobuterol / Tulobuterol 0,1–1 µg/kg CCα = 0,31 µg/kg CCβ = 0,42 µg/kg ±0,03 μg/kg (0,1 μg/kg) ±0,13 μg/kg (0,2 μg/kg) ±0,14 μg/kg (0,3 μg/kg) Zilpaterol / Zilpaterol 0,5–5 µg/kg CCα = 1,21 µg/kg CCβ = 1,42 µg/kg ±0,25 μg/kg (0,5 μg/kg) ±0,26 μg/kg (1,0 μg/kg) ±0,80 μg/kg (1,5 μg/kg) Presejalna metoda / Screening method Brombuterol / Brombuterol CCβ = 0,10 µg/kg Bromklorbuterol / Bromchlorbuterol CCβ = 0,10 µg/kg Cimaterol / Cimaterol CCβ = 0,25 µg/kg Cimbuterol / Cimbuterol CCβ = 0,25 µg/kg Klenbuterol / Clenbuterol CCβ = 0,025 µg/kg Klenciklohekserol / Clencyclohexerol CCβ = 0,50 µg/kg Klenhekserol / Clenhexerol CCβ = 0,50 μg/kg Klenpenterol / Clenpenterol CCβ = 0,25 µg/kg Klenproperol / Clenproperol CCβ = 0,25 µg/kg Fenoterol / Fenoterol CCβ = 0,50 µg/kg Izokssuprin / Isoxsuprine CCβ = 0,25 µg/kg Mabuterol / Mabuterol CCβ = 0,10 µg/kg Mapenterol / Mapenterol CCβ = 0,10 µg/kg Raktopamin / Ractopamine CCβ = 0,25 µg/kg Ritodrin / Ritodrine CCβ = 0,25 µg/kg Salbutamol / Salbutamol CCβ = 0,50 µg/kg Salmeterol / Salmeterol CCβ = 0,50 µg/kg Terbutalin / Terbutaline CCβ = 0,50 µg/kg Tulobuterol / Tulobuterol CCβ = 0,10 µg/kg Zilpaterol / Zilpaterol CCβ = 0,50 µg/kg

DA / YES


Določanje ergot alkaloidov v živilih in krmi; LC-MS/MS / Determination of ergot alkaloids; LC-MS/MS

Žita, krmne mešanice / Grains, compound feed 0,01–0,5 mg/kg LOD = 0,003 mg/kg LOQ = 0,01 mg/kg Ergokornin / Ergocornine 0,007 mg/kg (0,01 mg/kg)

DA / YES




Št. No.





Akreditirano*


0,10 mg/kg (0,2 mg/kg) 0,25 mg/kg (0,5 mg/kg) Ergokristin / Ergocristine 0,009 mg/kg (0,01 mg/kg) 0,14 mg/kg (0,2 mg/kg) 0,35 mg/kg (0,5 mg/kg) Ergokriptin / Ergocryptine 0,006 mg/kg (0,01 mg/kg) 0,11 mg/kg (0,2 mg/kg) 0,28 mg/kg (0,5 mg/kg) Ergometrin / Ergometrine 0,003 mg/kg (0,01 mg/kg) 0,14 mg/kg (0,2 mg/kg) 0,35 mg/kg (0,5 mg/kg) Ergozin / Ergosine 0,005 mg/kg (0,01 mg/kg) 0,05 mg/kg (0,2 mg/kg) 0,13 mg/kg (0,5 mg/kg) Ergotamin / Ergotamine 0,005 mg/kg (0,01 mg/kg) 0,05 mg/kg (0,2 mg/kg) 0,13 mg/kg (0,5 mg/kg) Ergokorninin / Ergocorninine 0,007 mg/kg (0,01 mg/kg) 0,03 mg/kg (0,2 mg/kg) 0,08 mg/kg (0,5 mg/kg) Ergokristinin / Ergocristinine 0,005 mg/kg (0,01 mg/kg) 0,05 mg/kg (0,2 mg/kg) 0,13 mg/kg (0,5 mg/kg) Ergokriptinin / Ergocryptinine 0,007 mg/kg (0,01 mg/kg) 0,05 mg/kg (0,2 mg/kg) 0,13 mg/kg (0,5 mg/kg) Ergometrinin / Ergometrinine 0,003 mg/kg (0,01 mg/kg) 0,03 mg/kg (0,2 mg/kg) 0,08 mg/kg (0,5 mg/kg) Ergozinin / Ergosinine 0,003 mg/kg (0,01 mg/kg) 0,06 mg/kg (0,2 mg/kg) 0,15 mg/kg (0,5 mg/kg) Ergotaminin / Ergotaminine 0,004 mg/kg (0,01 mg/kg) 0,05 mg/kg (0,2 mg/kg) 0,13 mg/kg (0,5 mg/kg)

86. Uredba Komisije (ES) št. 152/2009, Priloga VI, dopolnjena z Uredbo Komisije (EU) št. 51/2013 Commission Regulation (EC) No 152/2009, Annex VI, amended by Commission Regulation (EU) No 51/2013

Postopek za ugotavljanje prisotnosti tkiv živalskega izvora; mikroskopska metoda Analytical method for the determination of costituents of animal origin; microscopic method SOP 259, verzija 3

Krma in sestavine krme / Feed and feed ingredients Kvalitativna metoda / Qualitative method

DA / YES

87. Uredba Komisije (ES) št. 152/2009, Priloga IV, Metoda F Commission Regulation (EC) No 152/2009, Annex IV, Method F

Določanje diklazurila v krmi, premiksih in koncentratih; HPLC-UV detekcija Determination of diclazuril in feed, premixes and concentrates; HPLC-UV detection SOP 49, verzija 6

Krma / Feed Premiksi / Premixes Koncentrati / Concentrates 0,5 mg/kg–5 g/kg LOD = 0,2 mg/kg LOQ = 0,5 mg/kg 0,15 mg/kg (0,5 mg/kg) 0,30 mg/kg (1 mg/kg) 50 mg/kg (500 mg/kg)





Št. No.





Akreditirano*


0,03 % (5 g) 88. Uredba Komisije (ES) št.

152/2009, Priloga IV, Metoda G Commission Regulation (EC) No 152/2009, Annex IV, Method G

Določanje lasalocida v krmi, premiksih in koncentratih; HPLC-fluorescenčna detekcija Determination of lasalocid sodium in feed, premixes and concentrates; HPLC- fluorescence SOP 117, verzija 6

Krma / Feed Premiksi / Premixes Koncentrati / Concentrates 4,7 mg/kg–160 g/kg LOD = 1,2 mg/kg LOQ = 4,7 mg/kg 1 mg/kg (5 mg/kg) 5 mg/kg (100 mg/kg) 2,1 g/kg (20 g/kg) 1,2 % (160 g)


89. Uredba Komisije (ES) št. 152/2009, Priloga IV, Metoda E Commission Regulation (EC) No 152/2009, Annex IV, Method E

Določanje robenidina v krmi, premiksih in koncentratih; HPLC-UV detekcija Determination of robenidine in feed, premixes and concentrates; HPLC-UV detection SOP 118, verzija 6

Krma / Feed Premiksi / Premixes Koncentrati / Concentrates 3,2 mg/kg–66 g/kg LOD = 1,2 mg/kg LOQ = 3,2 mg/kg 1,2 mg/kg (3,2 mg/kg) 4,4 mg/kg (35,6 mg/kg) 6,3 mg/kg (64 mg/kg) 1,3 g/kg (8 g/kg) 0,9 % (66 g)



Določanje maduramicina v krmi, premiksih in koncentratih; HPLC-UV detekcija Determination of maduramicin ammonium in feed, premixes and concentrates; HPLC-UV detection

Krma / Feed Premiksi / Premixes Koncentrati / Concentrates 1,0 mg/kg–10 g/kg LOD = 0,4 mg/kg LOQ = 1,0 mg/kg 0,3 mg/kg (1 mg/kg) 1,9 mg/kg (5 mg/kg) 0,3 g/kg (1 g/kg) 0,2 % (10 g)


91. SOP 197 Interna metoda / In-house method

Določanje amprola; HPLC-UV detekcija Determination of amprolium; HPLC-UV detection

Krma / Feed Koncentrati / Concentrates 5 mg/kg–200 g/kg LOD = 1,1 mg/kg LOQ = 5 mg/kg 1,2 mg/kg (5,9 mg/kg) 2,1 mg/kg (29,4 mg/kg) 18 g/kg (200 g/kg)

NE / NO

92. Uredba Komisije (ES) št. 152/2009, Priloga III, Metoda C

Določanje surovih beljakovin Determination of crude proteins SOP 251, verzija 1

Krma / Feed

NE / NO

93. Uredba Komisije (ES) št. 152/2009, Priloga III, Metoda H

Določanje surovih maščob Determination of crude fats SOP 252, verzija 2

Krma / Feed NE / NO

94. Uredba Komisije (ES) št. 152/2009, Priloga III, Metoda I

Določanje surovih vlaknin Determination of crude fibre SOP 253, verzija 1

Krma / Feed

NE / NO

95. Uredba Komisije (ES) št. 152/2009, Priloga III, Metoda M

Določanje surovega pepela Determination of crude ash SOP 254, verzija 1

Krma / Feed

NE / NO

96. Uredba Komisije (ES) št. 152/2009, Priloga VI, dopolnjena z Uredbo Komisije (EU) št. 51/2013 Commission Regulation (EC) No 152/2009, Annex VI, amended by Commission Regulation (EU) No 51/2013

Postopek za ugotavljanje prisotnosti tkiv živalskega izvora; PCR Analytical method for the determination of costituents of animal origin; PCR

Hrana za ribe / Feed and feed material for aquaculture Kvalitativna metoda / Qualitative method

NE / NO

97. Metode IAG Mikroskopska preiskava krme / Microscopic feed examination SOP 380, verzija 1 SOP 396, verzija 1

Krma in sestavine krme / Feed and feed ingredients Kvalitativna metoda / Qualitative method

NE / NO




Št. No.





Akreditirano*


98. SOP 257 Interna metoda / In-house method

Določanje vitamina A in E; HPLC-UV detekcija / Determination of vitamin A and E; HPLC-UV detection

Krma / Feed NE / NO

Inštitut za mikrobiologijo in parazitologijo, Enota za bakteriologijo in mikologijo

Št. No.





Akreditirano* Da/Ne Accredited Yes/No

99. ISO 6579-1:2017

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella – 1. del: Ugotavljanje prisotnosti Salmonella spp. Microbiology of the food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 1: Detection of Salmonella spp. SOP 400, verzija 2

Krmila / Feedingstuffs LOD = (1–5) CFU/25 g Feces, vpojne prevleke za škornje, stelja, vzorci živalskega okolja in površin / Faeces, organs, boot swabs, litter, samples of animal environment and surfaces LOD = (5–10) CFU/25 g Bakterijski izolati / Bacterial isolates


100. Kauffmann-White-Le Minorjeva shema (WHO) Kauffmann- White-Le Minor scheme (WHO)

Serotipizacija sevov Salmonella enterica – Ugotavljanje somatskih in flagelarnih antigenov Serotyping of Salmonella enterica - Identification of somatic and flagellar antigens SOP 7, verzija 5

Bakterijski izolati / Bacterial isolates

DA / YES


Antibiogram – ugotavljanje občutljivosti bakterij za antibiotike in kemoterapevtike po mikrodilucijski metodi Antimicrobial susceptibility testing of bacteria – microdilution method Interna metoda v skladu s priporočili EURL za ugotavljanje odpornosti bakterij proti protimikrobnim zdravilom In-house method according to recommendations of EURL for antimicrobial resistance; Temelji na standardu EUCAST Based on EUCAST standard

Bakterijski izolati / Bacterial isolates merilna negotovost ± (0–2) log2 (glede na različne protimikrobne učinkovine) measurement uncertainty ± (0–2) log2 (regarding different antimicrobials)

DA / YES


Preiskava na povzročitelje goveje tuberkuloze (izolacija in identifikacija) Examination for aetiologic agents of bovine tuberculosis (isolation and identification) Temelji na / based on: OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.3.6. (avian tuberculosis), 2014 in/and 2.4.6. (bovine tuberculosis), 2009

Klinični in patološki vzorci / Clinical and pathologic samples LOD = 72 CFU/g Nastil, krma, voda / litter, feeding-stuffs, water

DA / YES NE / NO


Preiskava na bakterijo Listeria monocytogenes in drugih listerij – SOP 147: samo izolacija Examination on Listeria monocytogenes and Listeria spp. – SOP 147: detection only

Klinični in patološki vzorci, vzorci okolja / Clinical and pathologic samples, environment samples LOD = (5–10) CFU/25 g

DA / YES

104. ISO 11290-1:2017 samo potrditev confirmation only

Mikrobiologija v prehranski verigi – Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp. – 1. del: Metoda za ugotavljanje prisotnosti Microbiology of the food chain – Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria spp. – Part 1: Detection method SOP 147, verzija 8

Bakterijski izolati / Bacterial isolates DA / YES




Št. No.






105. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.3.11. (S. Gallinarum / Pullorum), 2018 in/and poglavje/chapter 2.9.8.(other Salmonellae), 2016

Preiskava kliničnega in patološkega materiala živalskega izvora ter vzorcev živalskega okolja na Salmonella spp. – izolacija in potrditev Examination of clinical and pathological samples of animal origin (terrestrial animals) and samples of animal environment for Salmonella spp. – isolation and confirmation SOP 15 (izolacija/isolation), verzija 6 SOP 400 (potrditev/confirmation), verzija 2

Klinični in patološki vzorci živalskega izvora ter vzorci živalskega okolja / Clinical and pathological samples of animal origine (terrestrial animals) and samples of aminal evironment LOD = (1–5) CFU/25g

DA / YES


Izolacija in identifikacija termotolerantnih Campylobacter spp. Isolation and identification of thermophilic Campylobacter spp. Temelji na ISO 10272-1:2017 - izolacija iz fecesa, izvaja se po tehničnih specifikacijah, navedenih v: Technical specifications set out in Annex I of the Commission Decision 2007/516/EC (Part C, 2.1.), identifikacija po ISO 10272-1 Based on ISO 10272-1:2017 – isolation from faeces according to Technical specifications set out in Annex I of the Commission Decision 2007/516/EC (Part C, 2.1.), and identification according to ISO 10272-1

Feces, organi, površine, bakterijski sevi / Faeces, organs, surfaces, bacterial strains LOD = 60 CFU/1 g

DA / YES

107. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.1.4., 2016

Rose bengal test za ugotavljanje protiteles proti bakterijam Brucella abortus, Brucella melitensis in Brucella suis Rose bengal test for detection of antibodies against Brucella abortus, Brucella melitensis and Brucella suis SOP 74, verzija 5

Serum domačih in divjih živali/ Serum of domestic and wild animalls

DA / YES


Dokaz nukleinskih kislin z metodo verižne reakcije s polimerazo v realnem času (real-time PCR) Metoda PCR v realnem času za ugotavljanje odsekov genomov živali in mikroorganizmov pri živalih Detection of nucleic acids using real-time polymerase chain reaction (real-time PCR) Real-time PCR method for the detection of animal genome regions and animal microorganisms genome regions

Molekularno dokazovanje bakterije Escherichia coli, ki proizvaja Šigove toksine (STEC) Molecular identification of STEC (Shiga toxin-producing Escherichia coli) SOP 461, verzija 4 Temelji na / based on: ISO 13136:2012 Molekularno dokazovanje bakterije Francisella tularensis Temelji na / based on: Fujita O, et al. (2006). Jpn J Infect Dis, 59: 46-51. Molekularno dokazovanje in determinacija izolatov brucel

DA / YES NE / NO NE / NO

109. ISO 13136:2012 Molekularno dokazovanje bakterije Escherichia coli, ki proizvaja Šigove toksine (STEC) Molecular identification of STEC (Shiga toxin-producing Escherichia coli) SOP 461, verzija 4 (faza obogatitve se izvaja na IVHKO, EVH / the enrichment step is performed at IVHKO, EVH)

Živila LOD = (0,95–10) CFU/25 g

DA / YES


Antibiogram – ugotavljanje občutljivosti bakterij za antibiotike in kemoterapevtike po difuzijski metodi z diski Antimicrobial susceptibilitity testing of bacteria – disc diffusion method

Temelji na standardih CLSI in EUCAST Based on CLSI and EUCAST standards

Bakterijski izolati / Bacterial isolates Ugotavljanje občutljivosti bakterij za protimikrobna zdravila Antimicrobial susceptibilitity testing of bacteria

NE / NO

111. ISO 16654

Preiskava na Escherichia coli O157 (izolacija in identifikacija) Detection of Escherichia coli O157 (isolation and identification)

SOP 278 (priprava in inokulacija mesa se opravlja na IVHKO, EVH / meat preparation and inoculation at IVHKO, EVH)

Meso / Meat LOD = (10–20) CFU/10 g

NE / NO




Št. No.







Preiskava na Escherichia coli O157 (izolacija in identifikacija) Detection of Escherichia coli O157 (isolation and identification) Temelji na: ISO 16654 / Based on: ISO 16654

Feces, vsebina črevesa, bakterijski sevi / Faeces, intestine content, bacterial strains LOD = (10–20) CFU/10 g

NE / NO


Molekularna identifikacija Bacillus anthracis - dokazovanje DNA bakterije s PCR v realnem času Temelji na: OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje 2.1.1.

Bakterijski izolati, prah, tkivo, vzorci okolja NE / NO

114. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje 2.1.15.

Postopek bakteriološke preiskave na paratuberkulozo - izolacija M. avium subsp. paratuberculosis

SOP 70

Organi, tkiva, iztrebki, krmila NE / NO


Molekularna identifikacija Coxiella burnetii - dokazovanje DNA bakterije s PCR v realnem času

Mleko, kri, organi, feces, vzorci okolja NE / NO


Preiskava na tularemijo - izolacija in determinacija Francisella tularensis

Kri in parenhimski organi zajcev, kuncev in drugih občutljivih živali

NE / NO


Preiskava na povzročitelja hude gnilobe čebelje zalege v medu - izolacija in ugotavljanje števila Paenibacillus larvae

Med ali satje z medom, drobir, vosek NE / NO


Preiskava na brucelozo – B. melitensis, B. abortus, B. suis, B. ovis - izolacija in preliminarna identifikacija do rodu

Organi, plod in plodove ovojnice, seme goveda, prašičev, ovc, koz in divjih živali

NE / NO


RVK bruceloza - metoda RVK za ugotavljanje prisotnosti protiteles proti bakterijam Brucella abortus, Brucella melitensis in Brucella suis SOP 477

Serum NE / NO


Hitra seroaglutinacija (SA) za ugotavljanje protiteles proti bakteriji Brucella canis

Serum NE / NO


Preiskava na govejo genitalno kampilobakteriozo - izolacija in identifikacija bakterije Campylobacter fetus subsp. venerealis

Biki: seme, prepucialni izpirek ali bris; Krave: vaginalna ali cervikovaginalna sluz, abortiran fetus, placenta

NE / NO


Preiskava na dermatofilozo - izolacija in determinacija Dermatophilus congolensis

Dlaka in kožni ostružki goveda NE / NO


Preiskava na Tritrichomonas foetus - dokaz z gojiščno preiskavo

Seme in prepucialni izpirek bika NE / NO


Preiskava na infekciozni metritis kopitarjev (metritis contagiosa equorum, CEM) - izolacija in determinacija bakterije Taylorella equigenitalis

Kobila: bris cerviksa, bris klitorisa, izcedek iz vagine ali maternice, placenta; Žrebec: bris uretre, fose uretralis, sluznice penisa ali prepucija, preejakulatna tekočina

NE / NO

125. Metoda EURL Preiskava na MRSA - Izolacija MRSA v kliničnem materialu in potrditev z ugotavljanjem občutljivosti za antibiotike

Klinični brisi, organi in tkiva, vzorci okolja NE / NO


Postopek bakteriološke preiskave na mikobakterije - izolacija mikobakterij SOP 69

Organi, tkiva, iztrebki, krmila NE / NO


Genotipizacija bakterij Escherichia coli - tipizacija izolatov s PFGE Temelji na: Caprioli A, et al. (2014). EFSA supporting publication: EN-704.

Bakterijski izolati NE / NO


Genotipizacija salmonel - tipizacija izolatov s PFGE Temelji na: Jacobs W et al. (2014). EFSA supporting publication: EN-703.





Št. No.







Genotipizacija Listeria monocytogenes – tipizacija izolatov s PFGE

Temelji na: Roussel S, et al. (2014). EFSA supporting publication: EN-702.



Genotipizacija Campylobacter jejuni, C. coli - tipizacija izolatov s PFGE Temelji na: Ribot EM, et al. (2001). J Clin Microbiol, 39: 1889-94.



Preiskava na botulizem - izolacija Clostridium botulinum in dokaz toksina

Temlji na: Quinn PJ, et al. Clinical veterinary microbiology, Mosby, 2002: 196-200.

Organi in vsebina prebavil občutljivih živali, vzorci okolja npr. blato, mulj; bakterijski izolati

NE / NO


Preiskava na skupno število klic v semenu - ugotavljanje števila živih bakterij v vzorcu (predvsem aerobov in fakultativnih ananerobov) Metoda štetja na komercialnem gojišču priznanem od AFNOR

Seme, prepucialni izpirki NE / NO


Preiskava na dermatofite - mikroskopsko dokazovanje dermatofitov v kužnini, izolacija in determinacija dermatofitov Temelji na: Larone DH. Medically important fungi, A guide to identification. Washington: American Society for Microbiology, 1993.

Dlaka, ostružek in bris kože NE / NO


Molekularna identifikacija Renibacterium salmoninarum – dokazovanje DNA bakterije s PCR

Organi (ledvica, vranica, jetra), bakterijski izolati NE / NO


Ugotavljanje genov Staphylococcus aureus (MRSA) – dokazovanje DNA bakterije z multipleks PCR



Metoda RVK za ugotavljanje protiteles proti protozoju Trypanosoma equiperdum SOP 76

Serum NE / NO

Inštitut za mikrobiologijo in parazitologijo, Enota za virologijo

Št. No.






137. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals chapter 2.4.7., 2015

Test ELISA za dokazovanje protiteles proti virusu bovine virusne diareje v serumu goveda ELISA test for detecting antibodies against the bovine viral diarrhoea virus in bovine sera SOP 246, verzija 6

Serum / Serum Mleko / Milk

DA / YES NE / NO

138. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.1.3., 2014 Protokol povzet po navodilih EURL IAH Pirbright according to the protocol of EURL IAH Pirbright

Reverzna transkripcija in verižna reakcija s polimerazo v realnem času (RT-qPCR) za dokaz nukleinske kisline virusa bolezni modrikastega jezika (BTV) Reverse transcription and polymerase chain reaction in real-time (RT-qPCR) for the detection of nucleic acids of bluetongue virus (BTV) SOP 339, verzija 4

Polna kri / whole blood DA / YES

139. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals chapter 2.8.3., 2014

Reverzna transkripcija in verižna reakcija s polimerazo v realnem času (RT-qPCR) za dokaz nukleinske kisline virusa klasične prašičje kuge (RT-qPCR KPK) Reverse transcription and real-time polymerase chain reaction to prove classical swine fever virus nucleic acid (RT-qPCR CSFV) SOP 370, verzija 6

Polna kri, serum, organi / whole blood, serum, organs

DA / YES




Št. No.






140. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.4.7., 2015

Reverzna transkripcija in verižna reakcija s polimerazo v realnem času (RT-qPCR) za dokaz nukleinske kisline virusa bovine virusne diareje (BVD) Reverse transcription and polymerase chain reaction in real-time (RT-qPCR) for the detection of nucleic acids of bovine viral diarrhoea virus (BVDV) SOP 465, verzija 3

Serum/ Serum Organi /Organs

DA / YES NE / NO


Dokaz protiteles proti virusu klasične prašičje kuge z metodo ELISA –komercialni komplet Detection of antibodies against classical swine fever by ELISA – commercial kit SOP 230, verzija 10

Serum / Serum Serološka preiskava za določitev protiteles proti virusu klasične prašičje kuge /Serological determination of antibodies against classical swine fever virus/ titer protiteles /antibody titer = 1: 10

DA / YES


Test ELISA za dokazovanje protiteles proti virusu Infekcioznega bovinega rhinotraheitisa / Infekcioznega bovinega pustulovulvovaginitisa v serumu goveda ELISA test for detecting antibodies against the infectious bovine rhinotracheitis/infectious bovine vulvovaginitis in bovine sera SOP 271, verzija 8

Serum / Serum Serološka preiskava za določitev protiteles proti virusu Infekcioznega bovinega rhinotraheitisa / Infekcioznega bovinega pustulovulvovaginitisa /Serological determination of antibodies against virus against the infectious bovine rhinotracheitis/infectious bovine vulvovaginitis virus/ Kvalitativna metoda/ Qualitative method

DA / YES


Dokaz protiteles proti virusu bolezni Aujeszkega z metodo ELISA – komercialni komplet Pseudorabies/Aujeszky disease virus Detection of antibodies against Aujeszky disease virus by ELISA – commercial kit Pseudorabies/Aujeszky disease virus SOP 311, verzija 5

Serum / Serum

DA / YES

144. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.1.2017

Test imunofluorescence (IF) za dokazovanje virusa stekline Fluorescent antibody test (FAT) for rabies virus diagnosis SOP 214, verzija 4

Odtis možganov/Brain smear

DA / YES


Dokaz protiteles proti virusu bolezni modrikastega jezika z metodo ELISA – komercialni komplet Detection of antibodies against Bluetongue virus by ELISA – commercial kit SOP 364, verzija 2

Serum / Serum

DA / YES

146. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.4.7. interna metoda in-house method

Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline virusa bovine virusne diareje (BVDV) Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of bovine viral diarrhoea virus (BVDV) SOP 277

Serum/ Serum Organi /Organs

NE/ NO NE/ NO

147. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.1.8. protokol povzet po navodilih referenčnega laboratorija EU, IAH Pirbright according to the protocol of EURL IAH Pirbright

Test LPB ELISA za določanje protiteles proti virusu slinavke in parkljevke tipov A, O, C LPB ELISA test for the detection of antibodies aginst foot and mouth disease against type A, O, C SOP 55

Serum / Serum

NE / NO




Št. No.







Reverzna transkripcija in verižna reakcija s polimerazo v realnem času (RT-qPCR) za dokaz nukleinske kisline virusa slinavke in parkljevke (SIP), vseh 7 serotipov Reverse transcription and polymerase chain reaction in real-time (RT-qPCR) for the detection of nucleic acids of foot and mouth disease (FMD), all 7 serotypes SOP 371

Vsebina afte, probang vzorec, epitelij kože / Vesicula fluid, probang sample, epithelium,

NE / NO

149. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.8.8.

Test ELISA za določanje protiteles proti virusu vezikularne bolezni prašičev (VBP) ELISA test for the detection of swine vesicular disease antibodies (SVD) SOP 319

Serum / Serum

NE / NO


Reverzna transkripcija in verižna reakcija s polimerazo v realnem času (RT-qPCR) za dokaz nukleinske kisline virusa vezikularne bolezni prašičev (VBP) Reverse transcription and polymerase chain reaction in real-time (RT-qPCR) for the detection of nucleic acids of swine vesicular disease (SVD) SOP 404

Vsebina afte, probang vzorec, epitelij kože / Vesicula fluid, probang sample, epithelium,

NE / NO


Test ELISA za določanje protiteles proti virusu afriške prašičje kuge (APK) ELISA test for the detection of african swine fever antibodies (ASF) SOP 308

Serum / Serum

NE / NO


Test ELISA za določanje virusa afriške prašičje kuge (APK) v prašičjem serumu ELISA test for the detection of african swine fever virus (ASF) SOP 423

Serum / Serum

NE / NO

153. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.8.1. protokol povzet po navodilih referenčnega laboratorija EU, CISA-INIA Valdeolmos according to the protocol of EURL CISA-INIA Valdeolmos

Verižna reakcija s polimerazo v realnem času (qPCR) za dokaz nukleinske kisline virusa afriške prašičje kuge (APK) Polymerase chain reaction in real-time (qPCR) for the detection of nucleic acids of african swine fever virus (ASF) SOP 405

Polna kri, serum, organi / Whole blood, serum, organs

NE / NO

154. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.8.1. protokol povzet po navodilih referenčnega laboratorija EU, CISA-INIA Valdeolmos according to the protocol of EURL CISA-INIA Valdeolmos

Verižna reakcija s polimerazo času (PCR) za dokaz nukleinske kisline virusa afriške prašičje kuge (APK) Polymerase chain reaction (PCR) for the detection of nucleic acids of african swine fever virus (ASF) SOP 340

Polna kri, serum, organi / Whole blood, serum, organs

NE / NO


Dokazovanje protiteles proti virusu afriške prašičje kuge (APK) z metodo imunobloting (IB) Detection of african swine fever virus abntibodies (ASF) using imunobloting methods SOP 403

Serum / Serum

NE / NO


Postopek testa ELISA za določanje specifičnih serumskih protiteles proti virusu bolezni modrikastega jezika - Blutoungue virus VP7 antibody kit Detection of antibodies against Bluetongue virus by ELISA – commercial kit Blutoungue virus VP7 antibody kit SOP 365

Serum / Serum

NE / NO




Št. No.







Test ELISA za dokazovanje protiteles proti virusu stekline (Biopro Rabies ELISA Ab test) ELISA test for the detection of rabies antibodies (Biopro Rabies ELISA Ab test) SOP 449

Serum / Serum

NE / NO


Postopek za dokazovanje protiteles proti virusu stekline z FAVN testom Flourescent antibody virus neutralisation test (FAVN) for the detection of antibodies against rabies SOP 321

Serum / Serum

NE / NO


Izolacija virusa stekline na celični kulturi Isolation of rabies virus on cell culture SOP 402

Vzorec možgan/ Brain sample NE / NO


Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline virusa stekline Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of rabies virus SOP 355

Vzorec možgan/ Brain sample NE / NO


Določanje titra stekline virusa SAD B19 v cepivu Fucsoral® Determination of titre of rabies virus SAD B19 in vaccine Fucsoral® SOP 426

Cepivo / Vaccine NE / NO


Izolacija in virusa IBR/IPV na celični kulturi Isolation of IBR/IPV on cell culture SOP 216

Organi, seme / organs, semen NE / NO

163. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.4.12

Test ELISA za dokazovanje protiteles proti virusu Infekcioznega bovinega rhinotraheitisa / Infekcioznega bovinega pustulovulvovaginitisa v serumu goveda ELISA test for detecting antibodies against the infectious bovine rhinotracheitis/infectious bovine vulvovaginitis in bovine sera SOP 361

Serum / Serum Serološka preiskava za določitev protiteles proti virusu Infekcioznega bovinega rhinotraheitisa / Infekcioznega bovinega pustulovulvovaginitisa /Serological determination of antibodies against virus against the infectious bovine rhinotracheitis/infectious bovine vulvovaginitis virus/ Kvalitativna metoda/ Qualitative method

NE / NO

164. OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.8.3. EU Commission decision (2002/106/EC), chapter VI C in EU Diagnostic Manual for Classical swine fever (CSF) diagnosis

Virus nevtralizacijski test (VNT) za dokaz protiteles proti virusu klasične prašičje kuge Virus neutralisation test (VNT) for the detection of antibodies against classical swine fever SOP 061

Serum / Serum

NE / NO


Virus nevtralizacijski test (VNT) za dokaz protiteles proti virusu bovine virusne diareje Virus neutralisation test (VNT) for the detection of antibodies against bovine viral diarrhoea virus SOP 306

Serum / Serum

NE / NO


Virus nevtralizacijski test (VNT) za dokaz protiteles proti virusu borderske bolezni ovac (BB) Virus neutralisation test (VNT) for the detection of antibodies against border disease virus (BDV) SOP 307

Serum / Serum

NE / NO




Št. No.







Virus nevtralizacijski test (VNT) za dokazovanje protiteles proti virusu Infekcioznega bovinega rhinotraheitisa / Infekcioznega bovinega pustulovulvovaginitisa v serumu goveda Virus neutralisation test (VNT) for detecting antibodies against the infectious bovine rhinotracheitis/infectious bovine vulvovaginitis in bovine sera SOP 419

Serum / Serum

NE / NO

168. 11

OIE Manual of Diagnostic Tests and Vaccines for Terrestiral Animals, chapter 2.8.3. EU Commission decision (2002/106/EC), chapter VI C in EU Diagnostic Manual for Classical swine fever (CSF) diagnosis

Izolacija virusa klasične prašičje kuge (KPK) na celični kulturi Isolation of classical swine fever (CSF) virus on cell culture SOP 060


NE / NO


Izolacija virusa BVD na celični kulturi Isolation of bovine viral diarrhoea virus on cell culture SOP 320


NE / NO


Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline pestivirusov in virusa klasične prašičje kuge (KPK) Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of pestiviruses and classical swine fever virus (CSF) SOP 293

Serum, organi / Serum, organs

NE / NO


Izolacija virusa kužnega arteritisa kopitarjev na celični kulturi Isolation of equine viral arteritis (EAV) on cell culture SOP 215

Laringotrahealni birs, abortirani fetus, sperma / Laringotracheal swabs, aborted foetuses, semen

NE / NO


Virus nevtralizacijski test (VNT) za dokaz protiteles proti virusu kužnega arteritisa kopitarjev Virus neutralisation test (VNT) for the detection of antibodies against equine viral arteritis (EAV) SOP 362

Serum / Serum

NE / NO


Izolacija in virusa bolezni Aujeskega na celični kulturi Isolation of Aujeszky disease virus on cell culture SOP 359

Organi / Organs

NE / NO


Virus nevtralizacijski test (VNT) za dokaz protiteles proti virusu bolezni Aujeszkega (BA) Virus neutralisation test (VNT) for the detection of antibodies against Aujeszky disease virus (AD) SOP 318

Serum / Serum

NE / NO


Dokaz protiteles proti virusu bolezni Aujeszkega z metodo ELISA – komercialni komplet Pseudorabies/Aujeszky disease virus kit PRV-gE-Ab (Svanova) Detection of antibodies against Aujeszky disease virus by ELISA – commercial kit Pseudorabies/Aujeszky disease virus kit PRV-gE-Ab (Svanova) SOP 452

Serum / Serum

NE / NO

176. OIE Manual of Diagnostic Tests for Aquatic Animals, chapters 2.3.4., 2018 and 2.3.10.

Izolacija in virusov VHS, IHN, in IPN na celični kulturi Isolation of VHS, IHN, in IPN on cell culture SOP 360

Organi, ovarialna tekočina / Organs, ovarial fluids

NE / NO




Št. No.






177. OIE Manual of Diagnostic Tests for Aquatic Animals, chapter 2.3.10., .

Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline virusne hemoragične septikemije (VHS) Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of viral haemorrhagic septicemia virus (VHS) SOP 352


NE / NO

178. OIE Manual of Diagnostic Tests for Aquatic Animals, chapter 2.3.4., .

Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline infekciozne hematopoetske nekroze (IHN) Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of infectious haematopoietic necrosis virus (IHN) SOP 353


NE / NO

179. SOP 354 Interna metoda in-house method

Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline nalezljive nekroze trebušne slinavke (IPN) Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of infectious pancreatic necrosis virus (IPN)


NE / NO


Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline virusa prašičjega reprodukcijskega in respiratornega sindroma (PRRS) Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of porcine reproductive and respiratory syndrome virus (PRRS) SOP 418

Organi, serum / Organs, serum

NE / NO

181. OIE Manual of Diagnostic Tests for Aquatic Animals, chapter 2.3.7.

Verižna reakcija s polimerazo (PCR) za dokaz nukleinske kisline KOI herpes virusa (KHV) Polymerase chain reaction (PCR) for the detection of nucleic acids of KOI herpes virus (KHV) SOP 429

Organi / Organs

NE / NO

182. SOP 406 Interna metoda / in-house method

Reverzna transkripcija in verižna reakcija s polimerazo (RT-PCR) za dokaz nukleinske kisline čebeljih virusov: ABPV, BQCV, CBPV, DWV, KBV, SBV Reverse transcription and polymerase chain reaction (RT-PCR) for the detection of nucleic acids of bee viruses: ABPV, BQCV, CBPV, DWV, KBV, SBV

Čebele mrtvice / dead bees NE / NO

183. SOP 457 Interna metoda / in-house method

Test ELISA za dokazovanje protiteles proti virusu Schmallenberg (ID Screen Schmallenberg virus indirect Multi species– komercialni komplet) ELISA test for the detection of Schmallenberg virus antibodies (ID Screen Schmallenberg virus indirect Multi species– commercial kit)

Serum / Serum

NE / NO

184. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, chapter 2.4.13. Interna metoda / in-house method

Verižna reakcija s polimerazo v realnem času (qPCR) za dokaz nukleinske kisline virusa vozličastega dermatitisa (LSD) Polymerase chain reaction in real-time (qPCR) for the detection of nucleic acids of Lumpy skin disease virus (LSD) SOP 487

Polna kri, brisi, organi / Whole blood, swabs, organs

NE / NO

185. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, chapter 2.5.6.

ELISA za ugot. protiteles proti virusu infekciozne anemije kopitarjev (IAK) - ugotavljanje specifičnih protiteles proti virusu SOP 229

Serum NE / NO

186. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, chapter 2.5.6.

AGID test za ugot. protiteles proti virusu infekciozne anemije kopitarjev (IAK) - ugotavljanje specifičnih protiteles proti virusu SOP 433

Serum NE / NO




Št. No.







Verižna reakcija s polimerazo v realnem času za dokaz nukleinske kisline virusa afriške prašičje kuge (qPCR APK), virotype® ASFV PCR Real time polymerase chain reaction for the detection of nucleic acids of african swine fever virus (qPCR ASF), virotype® ASFV PCR SOP 490

Polna kri, serum, organi, bris, suspenzija celične kulture / Whole blood, serum, organs, swabs, cell culture suspension

NE / NO


Test ELISA za dokazovanje protiteles proti virusu slinavke in parkljevke, tip A v serumu parklarjev (IZSLER FMD – Ab, type A) ELISA test for detecting antibodies against the Foot and mouth disease, serotype A (IZSLER FMD-Ab, type A) SOP 491

Serum / Serum

NE / NO

189. SOP 493 Interna metoda/ In-house method

Test ELISA za dokazovanje protiteles proti virusu hepatitisa E (PrioCHECK® HEV Ab porcine-komercialni komplet) ELISA for detection of hepatitis E antibodies (PrioCHECK® HEV Ab porcine-commercial kit)

Serum/Serum NE/NO

Inštitut za mikrobiologijo in parazitologijo, Enota za parazitologijo

Št. No.





Akreditirano* Da/Ne

Accredited Yes/No

190. Uredba komisije (ES) št. 2015/1375 Priloga I, Poglavje I, referenčna metoda odkrivanja (digestivna m.) Commission Regulation (EC) no 2015/1375; Annex I; Chapter I, reference method of detection, magnetic stirrer method for pooled sample digestion (digestive m.)

Preiskava na Trichinella spp. z digestivno metodo Examination for Trichinella spp. by digestive method SOP 8, verzija 8

Meso / Meat DA / YES


Preiskava na ehinokokozo - ugotavljanje ehinokoka na notranjih organih SOP 470

Notranji organi NE / NO


Preiskava na babeziozo - dokaz v krvnem razmazu obarvanem po Giemsi

Goveja kri z antikoagulansom NE / NO


Preiskava na cisticerkozo - dokazovanje prisotnosti cisticerkov v mišičnini SOP 488

Mišičnina NE / NO


Preiskava na garjavce - dokaz z mikroskopsko preiskavo kožnega ostružka po obdelavi s KOH

Kožni ostružek različnih vrst živali NE / NO



Inštitut za patologijo, divjad, ribe in čebele; Enota za patologijo ter sodno in upravno veterinarstvo

Št. No.



Materiali, proizvodi; Merilna negotovost (podana kot razširjena merilna negotovost U); Meje

metode Material, products; Measurement

uncertainty(expressed as expanded measurement uncertainty U); Method limits


195. Uredba (EC) št. 999/2001, Aneks III, Aneks X Regulation (EC) No. 999/2001, Annex III, Annex X

Postopek za diagnostiko bovine spongiformne encefalopatije (BSE) pri govedu in TSE pri drobnici s testom Prionics - Check WESTERN Procedure for the diagnostics of bovine spongiform encephalopathy (BSE )in cattle and TSE in sheep and goats with Prionics - Check WESTERN test SOP 168, verzija 11

Možgani goveda in drobnice / Bovine and small ruminant brain kvalitativna metoda/qualitative method

DA / YES

196. Uredba (EC) št. 999/2001, Aneks III, Aneks X Regulation (EC) No. 999/2001, Annex III, Annex X OIE Manual of diagnostic tests and vaccines for terrestrial animals,

Postopek za diagnostiko bovine spongiformne encefalopatije (BSE) pri govedu in TSE pri drobnici s patohistološko preiskavo možganov Procedure for the diagnostic of bovine spongiform encephalopathy (BSE) in cattle and TSE in sheep and goats with histopathology of the brain SOP 167, verzija 7


DA / YES

197. Uredba (EC) št. 999/2001, Aneks III, Aneks X Regulation (EC) No. 999/2001, Annex III, Annex X OIE Manual of diagnostic tests and vaccines for terrestrial animals,

Postopek za ugotavljanje prionov v možganih goveda in drobnice z imunohistokemično metodo Procedure for detecting prions in bovine and small ruminant brains by immunohistochemistry SOP 234, verzija 7


DA / YES

198. Uredba (EC) št. 999/2001, Aneks III, Aneks X Regulation (EC) No. 999/2001, Annex III, chapter A Annex X, Chapter C

Postopek za diagnostiko bovine spongiformne encefalopatije (BSE) pri govedu in TSE pri drobnici s testom Idexx HerdChek BSE-Scrapie Antigen (IDEXX Laboratories) Procedure for the diagnostics of bovine spongiform encephalopathy (BSE) in cattle and TSE in sheep and goats with the test Idexx HerdChek BSE-Scrapie Antigen (IDEXX Laboratories) SOP 177, verzija 6


DA / YES

Inštitut za predklinične vede; Enota za biokemijo, molekularno biologijo in genetiko

Št. No.







Genotipizacija gena PRNP pri ovcah s testom ločevanja alelov z metodo verižne reakcije s polimerazo v realnem času Sheep PRNP gene genotyping with allelic discrimination assay using real time polymerase chain reaction

Aleli 136, 141, 154 in 171 gena PRNP pri ovcah Alleles 136, 141, 154 and 171 of PRNP gene in sheep

DA / YES


Določevanje speciesa v mesu in procesirani hrani z metodo verižne reakcije s polimerazo v realnem času Species determination in meat and processed food by real time polymerase chain reaction

Določanje mitohondrijske DNA, specifične za konja (Equus cabalus); >1 % celokupne DNA Determination of mitochondrial DNA specific for horse (Equus cabalus); > 1 % total DNA

DA / YES



Inštitut za perutnino, ptice, male sesalce in plazilce

Št. No.






201. Council Directive 92/66/EEC, Annex 3 OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2-3-14, 2012

Postopek za test inhibicije hemaglutinacije za določitev protiteles proti virusu atipične kokošje kuge (APMV - 1) Procedure for haemagglutination inhibition test for detection of antibodies against Newcastle disease virus (APMV – 1) SOP 115, verzija 4

Serum perutnine / Poultry serum

DA / YES

202. Council Directive 2005/94/ES Manual of diagnostic for Avian Influenza (Commission decision 2006/437/ES) OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2-3-4, 2015

Postopek za test inhibicije hemaglutinacije za določitev protiteles proti virusu aviarne influence podtip H5 in H7 Procedure for haemagglutination inhibition test for detection of antibodies against Avian Influenza subtype H5 and H7 SOP 247, verzija 2


DA / YES

203. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.1.12, 2014 modificirana metoda modified method

Postopek za serološko preiskavo (test mikroskopske aglutinacije) na prisotnost protiteles proti leptospirozi Procedure for serological examination (microscopic agglutination test) for leptospiral antibodies SOP 120, verzija 4

Serum domačih živali / Serum of domestic animals

DA / YES

204. OIE; Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.3.5, 2015

Postopek za izvajanje testa hitre serumske aglutinacije za dokaz protiteles proti Mycoplasma gallisepticum Procedure for rapid serum agglutination test for detection antibodies against Mycoplasma gallisepticum SOP 275, verzija 2


DA / YES

205. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2.3.5, 2015

Ugotavljanje protiteles proti bakteriji Mycoplasma gallisepticum (MG) z metodo ELISA Procedure for rapid serum agglutination test for detection of antibodies against Mycoplasma gallisepticum (MG) by ELISA SOP 483, verzija 1


DA / YES


Izolacija virusne RNA Isolation of viral RNA

Klinični in patološki vzorci, Virusni izolati / Clinical and pathological samples, Virus isolates

DA / YES


Dokaz nukleinskih kislin z metodo verižne reakcije s polimerazo (real time - PCR) (metoda PCR v realnem času za ugotavljanje odsekov genomov živali in mikroorganizmov pri živalih) Detection of nucleic acids using real time polymerase chain reaction (real time – PCR) (Real time – PCR method for the detection of animal genome regions and animal microorganisms genome regions)

Odsek gena za protein matrix virusov influence tipa A, SOP 413 Region of matrix protein gene of influenza type A viruses, SOP 413 Odsek gena za protein hemaglutinin virusov influence podtipa H7, SOP 414 Region of hemagglutinin protein gene of H7 subtype influenza viruses, SOP 414 Odsek gena za protein hemaglutinin virusov influence podtipa H5, SOP 415 Region of hemagglutinin protein gene of H5 subtype influenza viruses, SOP 415 Odsek gena za protein neuraminidazo virusov influence podtipa N1, SOP 416 Region of neuraminidase protein gene of N1 subtype influenza viruses, SOP 416




Inštitut za perutnino, ptice, male sesalce in plazilce Št. No.






208. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2-3-4, 2015, Komisija evropskih skupnosti, 2006. Odločba komisije z dne 4. avgust 2006 o odobritvi diagnostičnega priročnika za aviarno influenco v skladu z Direktivo Sveta 2005/94/ES, 2006/437/ES. UL L 10, 14.1.2006, str. 16

Določanje gena za protein M virusov influence tipa A z metodo real-time Taqman RT-PCR Detection of protein M gene of type A influenza viruses by real-time Taqman RT-PCR SOP 413, verzija 3

Izolirana RNA / Isolated RNA DA / YES

209. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 2-3-4, 2015, Komisija evropskih skupnosti, 2006. Odločba komisije z dne 4. avgust 2006 o odobritvi diagnostičnega priročnika za aviarno influenco v skladu z Direktivo Sveta 2005/94/ES, 2006/437/ES. UL L 10, 14.1.2006, str. 16.

Določanje evrazijskih virusov aviarne influence podtipa H7 z metodo real-time Taqman RT-PCR Detection of Eurasian H7avian influenza viruses by real-time Taqman RT-PCR SOP 414 , ver.1



Določanje evrazijskih virusov aviarne influence podtipa H5 z metodo real-time Taqman RT-PCR Detection of Eurasian H5 avian influenza viruses by real-time Taqman RT-PCR SOP 415, ver.1



Določanje gena za protein N1 virusov aviarne influence z metodo real-time Taqman RT-PCR Detection of protein N1 gene of avian influenza viruses by real-time Taqman RT-PCR SOP 416, ver.1




Klinika za reprodukcijo in velike živali, Klinika za prežvekovalce in prašiče

Št. No.





Akreditirano* Da/Ne

Accredited Yes/No

212. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.8.6. –

ELISA za dokaz protiteles proti prašičjemu reprodukcijskemu in respiratornemu sindromu (PRRS) ELISA for detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) SOP 89

Serum / Serum NE / NO

213. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.9.9–

ELISA za dokaz protiteles proti salmonelam (serološke skupine O:4 (B), O:7 (C1), O:9 (D1)), pri prašičih ELISA test for detecting of antibodies against Salmonella (serogroups O:4 (B), O:7 (C1), O:9 (D1)) in swine SOP 358

Serum, mesni sok Serum, meat juice

NE / NO

Nacionalni veterinarski inštitut, Enota Maribor - Ptuj

Št. No.






214. ISO 6579-1:2017 Mikrobiologija v prehranski verigi -Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella – 1. del: Ugotavljanje prisotnosti Salmonella spp. Microbiology of the food chain - Horizontal method for the detection and enumeration of Salmonella spp. SOP 400, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = 5–10 CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = 5-10 CFU/25 g (ml) Jajca in jajčni izdelki / Eggs and egg products LOD = 5–10 CFU/vzorec Feces in vzorci okolja / Faeces and environmental samples (oz. vzorec vpojnih prevlek in brisov površin / or boot swab and surface sample) LOD = 2–3 CFU/25 g Kvalitativna metoda/qualitative method


215. SOP 368, verzija 2 Interna metoda In-house method

Postopek molekularnega ugotavljanje Salmonel v živilih, krmi, in vzorcih okolja s sistemom MDS Salmonella Molecullar detection system for detection of Salmonella in food, feed and food process environmental samples with MDS Salmonella

Vzorci prehranske verige LOD = 3-5 CFU/25 g Vzorci primarne proizvodnje Kvalitativna metoda/qualitative method

DA / YES NE / NO

216. ISO 11290-1:2017


Meso in mesni izdelki / Meat and meat products LOD = 5–10 CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = 5–10 CFU/25 g (ml) Brisi okolja / environmental swabs LOD = 7 CFU/površina


217. ISO 11290-2: 2017

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 2.del: Ugotavljanje števila Microbiology of the food chain - Horizontal method for detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method SOP 314, verzija 3

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,18 log10CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) U= ± 0,24 log10CFU/g (tekočine/liquids) U= ± 0,24 log10CFU/g (trdni vzorci/solid milk products)

DA / YES DA / YES




Št. No.






218. ISO 6888-1:1999

Preiskava na koagulaza pozitivne stafilokoke Enumeration of coagulase-positive staphylococci (S. aureus and other species) by Baird-Parker agar medium SOP 203, verzija 3

Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) U= ± 0,09 log10CFU/g (tekočine/liquids) U= ± 0,07 log10CFU/g (trdni vzorci/solid milk products) Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,11 log10CFU/g

DA / YES DA / YES

219. ISO 4833-1: 2013


Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,11 log10CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) U= ± 0,10 log10CFU/g (tekočine/liquids) U= ± 0,11 log10CFU/g (trdni vzorci/solid milk products)

DA / YES DA / YES

220. ISO 16649-2 : 2001

Določanje števila β glukoronidaza pozitivnih bakterij Escherichia coli Kvantitativna metoda Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of β glucuronidase-positive Escherichia coli Part 2:Colony count technique at 44o C SOP 315, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,09 log10CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) U= ± 0,14 log10 CFU/g (tekočine/liquids) U= ± 0,2 log10 CFU/g (trdne snovi/solid milk products)

DA / YES DA / YES

221. ISO 21528-2:2017 Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnost in števila enterobakterij - 2.del: Metoda štetja kolonij Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2: Colony count technique SOP 317, verzija 3

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,18 log10CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) U= ± 0,21 log10 CFU/g Brisi in izsečki trupov / Environmental swabs LOD = 10 CFU/cm2 U= ± 0,18 log10 CFU/cm2 Jajca in jajčni izdelki / Eggs and eggs products LOD = 10 CFU/g (ml)

DA / YES DA / YES DA/YES NE / NO


Meso in mesni izdelki / meat and meat products LOD = 2–3 CFU/g

DA / YES


Izolacija in identifikacija termotolerantnih Campylobacter spp. Isolation and identification of thermophilic Campylobacter spp. Temelji na ISO 10272-1 - izolacija iz fecesa, izvaja se po tehničnih specifikacijah, navedenih v: Technical specifications set out in Annex I of the Commission Decision 2007/516/EC (Part C, 2.1.), identifikacija po ISO 10272-1 Based on ISO 10272-1 – isolation from faeces according to Technical specifications set out in Annex I of the Commission Decision 2007/516/EC (Part C, 2.1.), and identification according to ISO 10272-1

Feces, organi, površine / Faeces, organs, surfaces LOD = 60 CFU/g

DA / YES




Št. No.








Serum / Serum Serološka preiskava za določitev protiteles proti virusu klasične prašičje kuge /Serological determination of antibodies against classical swine fever virus/ titer protiteles /antibody titer = 1: 10

DA / YES



Serum / Serum

DA / YES




Št. No.







Ugotavljanje zaviralnih substanc v mleku (DELVOTEST®SP-NT) Detection of the presence of inhibitory substances in milk (DELVOTEST®SP-NT)

mleko / milk penicilin G / penicillin G CCβ = 3 µg/kg ampicilin / ampicillin CCβ = 4 µg/kg amoksicilin / amoxicillin CCβ = 4 µg/kg kloksacilin / cloxacillin CCβ = 20 µg/kg nafcilin / nafcillin not sensitive cefapirin / cefapirin not sensitive cefkvinom / cefquinome not sensitive cefalonij / cefalonium CCβ = 20 µg/kg cefoperazon / cefoperazone CCβ = 50 µg/kg ceftiofur / ceftiofur CCβ = 50 µg/kg cefazolin / cefazolin CCβ = 10 µg/kg cefaleksin / cefalexin CCβ = 70 µg/kg tetraciklin / tetracycline very low sensitivity oksitetraciklin / oxytetracycline very low sensitivity klortetraciklin / chlortetracycline not sensitive doksiciklin / doxycycline not sensitive neomicin / neomycin CCβ = 500 µg/kg streptomicin / streptomycin very low sensitivity dihidrostreptomicin / dihydrostreptomycin very low sensitivity gentamicin / gentamicin not sensitive kanamicin / kanamycin not sensitive kinoloni / quinolones not sensitive sulfatiazol / sulfathiazole CCβ = 90 µg/kg sulfadiazin / sulfadiazine very low sensitivity sulfadoksin / sulfadoxin very low sensitivity eritromicin / erythromycin very low sensitivity linkomicin / lincomycin very low sensitivity novobiocin / novobiocin very low sensitivity rifaksimin / rifaximin very low sensitivity spiramicin / spiramycin very low sensitivity tilozin / tylosin CCβ = 50 µg/kg bacitracin / bacitracin very low sensitivity

DA / YES




Št. No.









228. ISO 10272-2 : 2017


Meso in mesni izdelki Meat and meat products LOD = 10 CFU/g ± 0,23 log10 CFU/g

DA / YES

229. OIE Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees), poglavje 2.2.2



230. Larone D.H., Medically important Fungi, A guide to identification. Washington: American Society for Microbiology

Preiskava na dermatofite - mikroskopsko dokazovanje dermatofitov v kužnini, izolacija in determinacija dermatofitov


231. SOP 147: 1. del Interna metoda SOP147: part 1 In-house method

Preiskava na bakterijo Listeria monocytogenes - izolacija Isolation of Listeria monocytogenes SOP 147: 1. del/part 1 potrditev/confirmation: SOP 147, 2. del/part 2

Klinični in patološki vzorci / Clinical and pathologic samples LOD = 5–10 CFU/25 g

NE / NO



Nacionalni veterinarski inštitut, Enota Novo mesto

Št. No.






232. SOP 237, verzija 6 Interna metoda/in-house method



DA / YES




Št. No.









234. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.1.4, 2016


Serum domačih in divjih živali/ Serum of domestic and wild animals

DA / YES

235. Manual of Diagnostic Tests and Vaccines for Terrestiral Animals. World organisation for animal health Chapter 2.1.3 – last version on O.I.E. web-page

Dokaz protiteles proti virusu bolezni modrikastega jezika z metodo ELISA – komercialni komplet INGEZIM BTV DR (Ingenasa) Detection of antibodies against Bluetongue virus by ELISA – commercial kit INGEZIM BTV DR (Ingenasa) SOP 364

Serum / Serum

NE / NO

236. ISO 6888-1 Preiskava na koagulaza pozitivne stafilokoke Enumeration of coagulase-positive staphylococci (S. aureus and other species) by Baird-Parker agar medium SOP 203

Meso in mesni izdelki / Meat and meat products LOD = 100CFU/g u=24,06%

NE / NO

237. ISO 6579-1:2017

Mikrobiologija v prehranski verigi -Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella – 1. del: Ugotavljanje prisotnosti Salmonella spp. Microbiology of the food chain - Horizontal method for the detection and enumeration of Salmonella spp. SOP 400, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = 5–10 CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = 5 CFU/25 g (ml) Feces in vzorci okolja / Faeces and environmental samples (oz. vzorec vpojnih prevlek in brisov površin or boot swab and surface sample) LOD = 5–10 CFU/25 g Kvalitativna metoda/qualitative method


238. ISO 4833-1

Ugotavljanje skupnega števila mikroorganizmov (SŠMO) z metodo štetja na ploščah pri 30 oC Enumeration of the number of microorganisms at 30 oC by colony count technique SOP 206

Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/ml ±30,6% Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g ±37,0%

NE / NO NE / NO

239. SOP 243 Interna metoda/in-house method

Preiskava na kloramfenikol v surovem mleku z ELISA Randox testom Determination of chloramphenicol residues in raw milk by ELISA Randox test presejalna metoda/screening method

Mleko / Milk CCβ=0,27μg/kg

NE / NO




Št. No.






240. SIST EN ISO 8655-6 Interne kalibracije pipet – za notranje uporabnike V 180

Prostornina tekočin/Volume of Liquids Pipete/Pipettes najboljša merilna zmoglijivost/Best measurement Capability (BMC)

NAZIVNA PROSTORNINA BMC

1 µl 0,09 µl 2 µl 0,09 µl 5 µl 0,09 µl 10 µl 0,09 µl 20 µl 0,09µl 50 µl 0,10 µl 100 µl 0,16 µl 300 µl 0,16 µl 500 µl 0,26 µl 1000 µl 0,35 µl 2000 µl 1,94 µl 5000 µl 4,60 µl 10000 µl 7,59µl

NE / NO




242. Larone D.H., Medically important Fungi, A guide to identification. Washington: American Society for Microbiology

Preiskava na dermatofite - mikroskopsko dokazovanje dermatofitov v kužnini, izolacija in determinacija dermatofitov


243. OIE Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees), Poglavje 2.1.1

Preiskava na vranični prisad - ugotavljanje okužbe in kontaminacije z bakterijo Bacillus anthracis

Kri, organi, edemska tekočina, peritonealna tekočina, koža; dlaka, volna

NE / NO

244. ISO 11290-1

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 1. del: Ugotavljanje prisotnosti Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria spp.- Part 1: Detection method SOP 162

meso in mesni izdelki / meat and meat products mleko in mlečni izdelki / milk and milk products brisi okolja / environmental swabs


245. ISO 11290-2 Amendment 1

Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 2.del: Ugotavljanje števila Microbiology of the food chain - Horizontal method for detection and enumeration of Listeria monocytogenes and of Listeria spp. - Part 2: Enumeration method SOP 314

Meso in mesni izdelki / Meat and meat products Mleko in mlečni izdelki / Milk and milk products

NE/NO NE/NO

246. ISO 21528-2 Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnost in števila enterobakterij - 2.del: Metoda štetja kolonij Microbiology of the food chain - Horizontal method for the detection and enumeration of Enterobacteriaceae - Part 2: Colony count technique SOP 317


NE/NO NE/NO


Preiskava na bakterijo Listeria monocytogenes - izolacija Isolation of Listeria monocytogenes SOP 147: 1. del/part 1 potrditev/confirmation: glej/see SOP 147, 2. del/part 2

Klinični in patološki vzorci / Clinical and pathologic samples

NE/NO




Št. No.






248. ISO 16649-2

Določanje števila ß glukoronidaza pozitivnih bakterij Escherichia coli Kvantitativna metoda Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of ß glucuronidase-Escherichia coli Part 2:Colony count technique at 44o C SOP 315


NE/NO NE/NO

Nacionalni veterinarski inštitut, Enota Nova Gorica

Št. No.






249. ISO 6579-1:2017


Meso in mesni izdelki / Meat and meat products LOD = (5–10) CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = (5–10) CFU/25 g (ml) Jajca in jajčni izdelki / Eggs and eggs products LOD=(5-10) CFU/25 g Feces in vzorci okolja / Faeces and environmental samples LOD = (5–8) CFU/25 g (oz. vzorec vpojnih prevlek in brisov površin or boot swab and surface sample) Kvalitativna metoda/Qualitative method


250. ISO 11290-1:2017


Meso in mesni izdelki / Meat and meat products LOD = (5–10) CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = (5–10) CFU/25 g (ml) Brisi okolja / environmental swabs LOD = 5-10 CFU/površina


251. ISO 11290-2: 1998 Amendment 1: 2004

Preiskava na Listeria monocytogenes Kvantitativna metoda Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Listeria monocytogenes Part 2: enumeration method SOP 314, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml)

DA / YES DA / YES

252. ISO 4833-1: 2013


Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) ±11,7% Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g ±18,16%

DA / YES DA / YES




Št. No.






253. ISO 16649-2:2001 Določanje števila β glukoronidaza pozitivnih bakterij Escherichia coli Kvantitativna metoda Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of β glucuronidase-positive Escherichia coli Part 2:Colony count technique at 44o C SOP 315, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,07 log10CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) U= ± 0,07 log10CFU /ml (tekočine,liquids) U= ± 0,10 log10CFU/g (trdne snovi, solid milk products)

DA / YES DA / YES

254. ISO 21528-2:2004 Preiskava na Enterobacteriaceae – števna metoda Enumeration of Enterobacteriaceae SOP 317, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) Brisi in izsečki trupov LOD = 10 CFU /cm2 Jajca in jajčni izdelki / Eggs and eggs products LOD = 10 CFU/g (ml)

DA / YES DA / YES DA / YES NE / NO


Meso in mesni izdelki / Meat and meat products LOD = (5–8) CFU/10 g

DA / YES

256. ISO 10272-2 : 2017


Meso in mesni izdelki Meat and meat products LOD = 10 CFU/g

DA / YES

257. SOP 279 verzija 4 Interna metoda In-house method

Izolacija in identifikacija termotolerantnih Campylobacter spp. Isolation and identification of thermophilic Campylobacter spp. Temelji na ISO 10272-1 - izolacija iz fecesa, izvaja se po tehničnih specifikacijah, navedenih v: Technical specifications set out in Annex I of the Commission Decision 2007/516/EC (Part C, 2.1.), identifikacija po ISO 10272-1 Based on ISO 10272-1 – isolation from faeces according to Technical specifications set out in Annex I of the Commission Decision 2007/516/EC (Part C, 2.1.), and identification according to ISO 10272-1

Feces, organi, površine / Faeces, organs, surfaces LOD = 60 CFU/g

DA / YES



Meso / Meat DA/YES




Št. No.









DA / YES

260. ISO 6888-1

Preiskava na koagulaza pozitivne stafilokoke Enumeration of coagulase-positive staphylococci (S. aureus and other species) by Baird-Parker agar medium SOP 203

Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g ±21,5%

NE / NO

261. IDF 108B ISO 5764

Postopek za ugotavljanje zmrziščne točke mleka s krioskopom Determination of freezing point – Thermistor cryoscope method SOP 242

Kravje mleko / cow milk -408 do -600 m °C r ≤ 4 m°C R ≤ 6 m°C

NE / NO







Št. No.







Preiskava na bakterijo Listeria monocytogenes - izolacija Isolation of Listeria monocytogenes SOP 147: 1. del/part 1 potrditev/confirmation: glej/see SOP 147, 2. del/part 2

Klinični in patološki vzorci / Clinical and pathologic samples LOD = (5–10) CFU/25 g

NE/ NO

264. OIE Manual of Standards for Diagnostic Tests and Vaccines for Terrestrial Animals (mammals, birds and bees), poglavje 2.5.12 in 2.9.8

Preiskava na garjavce - dokaz z mikroskopsko preiskavo kožnega ostružka po obdelavi s KOH

Kožni ostružek različnih vrst živali NE / NO

Nacionalni veterinarski inštitut, Enota Celje

Št. No.






265. ISO 6579-1:2017 Mikrobiologija v prehranski verigi -Horizontalna metoda za ugotavljanje prisotnosti, števila in serotipov Salmonella – 1. del: Ugotavljanje prisotnosti Salmonella spp. Microbiology of the food chain - Horizontal method for the detection and enumeration of Salmonella spp. SOP 400, verzija 2

Meso in mesni izdelki / Meat and meat products LOD = (5–10) CFU/25 g Feces, organi, vpojne prevleke za škornje, stelja, vzorci živalskega okolja in površin / Faeces, organs, boot swabs, litter, samples of animal environment and surgaces LOD = 9 CFU/25g Kvalitativna metoda /Qualitative method

DA / YES DA / YES

266. ISO 11290-1:2017 Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 1. del: Ugotavljanje prisotnosti Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria spp.- Part 1: Detection method SOP 162, verzija 5

Meso in mesni izdelki / Meat and meat products LOD = ≤8 CFU/25 g

DA / YES

267. ISO 4833-1: 2013


Meso in mesni izdelki / Meat and meat products LOD = 10 CFU/g U= ± 0,102 log10 CFU/g.

DA / YES

268. ISO 16649-2:2001


Meso in mesni izdelki / Meat and meat products: LOD = 10 CFU/g U= ± 0,10 log10CFU/g

DA / YES







Št. No.






270. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, poglavje/chapter 2.1.4, 2016


Serum domačih in divjih živali/ Serum of domestic and wild animalls

DA / YES

271. ISO 6888-1 Preiskava na koagulaza pozitivne stafilokoke Enumeration of coagulase-positive staphylococci (S. aureus and other species) by Baird-Parker agar medium SOP 203

Meso in mesni izdelki / Meat and meat products LOD = 100 CFU/g U=20,18%

NE / NO




Št. No.






272. SOP 237 interna metoda/ in-house method



NE / NO



Nacionalni veterinarski inštitut, Enota Kranj

Št. No.


number






Postopek ugotavljanja števila bakterij v mleku s pomočjo fluoro-opto-elektronskega inštrumenta Bactoscan Enumeration of bacteria in milk with fluoro-opto-electronic instrument Bactoscan

Surovo mleko / Raw milk 3.000–1.000.000 CFU/ml

DA / YES




DA / YES




Št. No.


number








276. ISO 13366-2:2006

Ugotavljanje števila somatskih celic v mleku s fluoro-opto-elektronskim aparatom Enumeration of somatic cells –Fluoro-opto-electronic method SOP 239, verzija 4

Surovo mleko / Raw milk 5.000–1.500.000 somatskih celic /ml

DA / YES

277. IDF 108B:2009 ISO 5764:2009

Ugotavljanje zmrziščne točke mleka s krioskopom Determination of freezing point – Thermistor cryoscope method SOP 242, verzija 7

Kravje mleko / Cow Milk -408 do -600 m °C ± 2 m°C

DA / YES

278. ISO 9622:2013 (IDF 141)

Ugotavljanje vsebnosti mlečne maščobe, beljakovin, laktoze in zmrziščne točke v mleku s pomočjo IR spektrometra Determination of milkfat, protein, lactose content and freezin point with mid-infrared spectrometry SOP 245, verzija 8

Surovo mleko / Raw milk maščobe/milk fat (2,0 do 5,5) g/100 ml beljakovine/proteini (2,5 do 4,3) g/100ml laktoza/lactose (4,2 do 4,9) g/100g Zmrziščna točka/freezing point (-0,458°C do -0,522°C)

DA / YES DA / YES

279. ISO 6579-1:2017


Meso in mesni izdelki / Meat and meat products LOD = (<3) CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = (<6) CFU/25 g (ml) Feces, organi, vpojne prevleke za škornje, stelja, vzorci živalskega okolja in površin / Faeces, organs, boot swabs, litter, samples of animal environment and surgaces LOD = 5 CFU/25g



Preiskava na Salmonella spp – modificirana IRIS metoda Horizontal method for the detection of Salmonella spp – modified IRIS method

Meso in mesni izdelki / Meat and meat products LOD = (1-5) CFU/sample 10 or 25 g Mleko in mlečni izdelki / Milk and milk products LOD = (1–5) CFU/25 g (ml) Brisi trupov / Environmental swabs LOD = (1–5) CFU/vzorec

DA / YES

281. ISO 11290-1:2017


Meso in mesni izdelki / Meat and meat products LOD = < 7 CFU/25 g Mleko in mlečni izdelki / Milk and milk products LOD = < 7 CFU/25 g (ml)

DA / YES DA / YES




Št. No.


number





282. ISO 16649-2:2001


Meso in mesni izdelki / Meat and meat products LOD =10 CFU/g U=±0,15 log10CFU /g Mleko in mlečni izdelki / Milk and milk products LOD =10 CFU/g poltekoči mlečni izdelki (well-mixed solids); U=±0,16 log10CFU /ml(g) trdi mlečni izdelki (other solids) U= ±0,12 log10CFU /g

DA / YES DA / YES

283. ISO 4833-1:2013


Mleko in mlečni izdelki / Milk and milk products LOD = 10 CFU/g (ml) ±U=0,24 log10 CFU /ml

DA / YES



Nacionalni veterinarski inštitut, Enota Murska Sobota

Št. No.


number





284. ISO 6579-1:2017


Meso in mesni izdelki / Meat and meat products LOD = 5–10 CFU/25 g Feces, organi, vpojne prevleke za škornje, stelja, vzorci živalskega okolja in površin / Faeces, organs, boot swabs, litter, samples of animal environment and surgaces LOD = 2 CFU/25g Kvalitativna metoda/qualitative method

DA / YES DA / YES

285. ISO 11290-1:2017 Mikrobiologija v prehranski verigi - Horizontalna metoda za ugotavljanje prisotnosti in števila Listeria monocytogenes in Listeria spp.- 1. del: Ugotavljanje prisotnosti Microbiology of the food chain - Horizontal method for the detection and enumeration of Listeria monocytogenes and Listeria spp.- Part 1: Detection method SOP 162, verzija 5

Meso in mesni izdelki / Meat and meat products LOD=5–10 CFU/25 g

DA / YES

286. ISO 4833-1: 2013


Meso in mesni izdelki / Meat and meat products LOD=10 CFU/g U=±0,27 log10CFU /g

DA / YES

287. ISO 16649-2:2001


Meso in mesni izdelki / Meat and meat products: LOD = 10 CFU/g U= ± 0,17 log10CFU/g

DA / YES







Št. No.


number





289. SOP 237, verzija 6 Interna metoda/ in–house method



DA / YES

290. SOP 243 Interna metoda/in-house method

Preiskava na kloramfenikol v surovem mleku z ELISA Randox testom Determination of chloramphenicol residues in raw milk by ELISA Randox test Presejalna metoda/screening method

Mleko / Milk CCβ=0,25μg/ml

NE / NO




Št. No.


number







Serum prašičev / Pork serum

DA / YES



Serum prašičev / Pork serum

DA / YES

Nacionalni veterinarski inštitut (NVI), Gerbičeva 60, 1000 Ljubljana

Št. No.


number






Tehnike vzorčenja za mikrobiološke preiskave živil skladno z osnutkom ISO TS 17728, ter mleka in mlečnih izdelkov skladno z ISO 707 Sampling techniques for microbiological analysis of food samples according to ISO TS 17728 and for milk and dairy products according to ISO 707

Živila živalskega izvora / Food of animal origin

DA / YES


Tehnike vzorčenja površin okolja skladno z ISO 18593 in Smernicami o vzorčenju živilsko predelovalnih območij in opreme za detekcijo bakterije Listeria monocytogenes Sampling techniques for environmental surfaces according to ISO 18593 and Guidelines on sampling the food processing area and equipment for the detection of Listeria monocytogenes

Vzorci okolja / Environmental samples DA / YES

295. Uredba Komisije (ES) št. 152/2009, Uredba Komisije (EU) št. 691/2013, Uredba Komisije (EU) št. 142/2011, Priloge IX, X, XIII Commission Regulation (EC) No 152/2009, Commission Regulation (EU) No 691/2013, Commission Regulation (EU) No 142/2011, Annexes IX, X, XIII

Vzorčenje krme Sampling of feed SOP 467, verzija 1

Krma / Feed DA / YES

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