Presented by Qiong Cheng in cs6640

Instructor: Prof. Irene T. Weber Prof. Robert W. Harrison

Identify an Obesity Association INSIG2

Outline

Introduction Analysis Methods Analysis Results Conclusion

Introduction Obesity

- an excess of body fat- a known risk factor for chronic diseases- a kind of metabolic disease

INSIG Family and INSIG2

INSIG Family’s Role

INSIG2 is an attractive candidate protein which product inhibits the synthesis of fatty acid and cholesterol [1].

Analysis Methods NCBI gene/protein database searching Blast Alignment searching in PDB

database PDB database searching LALIGN for pairwise alignment ClustalW for multiple alignment Newho + preammp + AMMP structure

alignment tools Rasmol to show protein structure

Analysis Results NCBI-Result

INSIG2 sequence1 maegetespg pkkcgpyiss vtsqsvnlmi

rgvvlffigv flalvlnllq iqrnvtlfpp61 dviasifssa wwvppccgta savigllypc

idrhlgephk fkrewssvmr cvavfvginh121 asakvdfdnn iqlsltlaal siglwwtfdr

srsgfglgvg iaflatvvtq llvyngvyqy181 tspdflyvrs wlpciffagg itmgnigrql

amyeckviae kshqe//

Analysis Results BLAST-Result

Alignment of INSIG2 and 1R08 in LALIGN

Alignment of INSIG2 and 1RHI in LALIGN

Alignment of INSIG2, 1R08 and 1RHI in ClustalW

Alignment Tree Generated in ClustalW

Analysis Results AMMP-Predicted INSIG2

Molecule Display--- Ribbons Display

Analysis Results AMMP-Predicted INSIG2

Molecule Display--- Ribbons Display

Analysis Results Comparison-1 in RasMol

Predicted INSIG2 --- 1R08 in 3rd chain

Analysis Results Comparison-2 in RasMol

The predicted INSIG2 is the red one; 1R08 in 3rd Chain is the green one;

Analysis Results Comparison-2 in RasMol

The predicted INSIG2 is the red one; 1R08 in 3rd Chain is the green one;

Analysis Results The longest gap

Seek motif SAK Seek the first

residue after the largest gap

Identify the gap

[in RasMol Tool ]

Conclusion Study research topics and tools in

bioinformatics; Identify INSIG2 structure; The interesting thing is macromolecule structure

homology; Further experiments and identification are

needed;

References: Alan Herbert, Norman P.Gerry A Common

Genetic Variant is Associated with Adult and Childhood Obesity Science, Vol312 (2006).

…

Analysis Results Sequence Alignment

Alignment of INSIG2 and 3rd chain of 1R08 (HRV14)