Presented by:  Grant Washing‐SmithDirector

Anagenix Ltd.

Traceability and AuthenticityTraceability and Authenticity

Growing Region 

Production and Manufacture

What makes Actazin Unique?

Actazin has a Four-Way action

What makes Actazin Unique?

yon gut health.

Prokinetic Enzyme activity gently stimulates gut motility and helps normalize bowel

Prebiotic

and helps normalize bowel transit time

Prebiotics support the growth of beneficial bacteria in the gut

FibrePoly‐phenolics

in the gut

Insoluble Fiber promotes regularity and softens stools without gas or bloating

Polyphenolics support beneficial bacterial, inhibit pathogens and maintains long term digestive health

Enzymes

g g

Four-Way action on Gut Health

Actazin is a new digestive supplement ingredient, manufactured fromN Z l d' f i h ki if i hi h h b li i ll

Four Way action on Gut Health

New Zealand's own superfruit, the kiwifruit, which has been clinicallyproven to support gut health by improving regularity and other longterm digestive health benefits.

Actazin is a unique product as it offers a combination of Four of the keyelements essential for good digestion --

St d di d Ki i E (SKE) Standardized Kiwi Enzymes (SKE), Prebiotics Fiber Polyphenolics (phytonutrients and antioxidants)

which are all derived from one natural ingredient; the New Zealandgreen kiwifruit.

The Power to Regenerate

Kiwi fruit possesses the unique ability to regenerate its cells.The enzyme complex found in Actazin which is responsible

The Power to Regenerate

The enzyme complex found in Actazin, which is responsibleStandardized Kiwi Enzymes (SKE), also interacts with receptors inhuman gut-lining cells.

Thus Actazin™ supports regeneration of gut lining cells asThus, Actazin™ supports regeneration of gut-lining cells, aswell as enhanced gut lining protection and increased gutmotility.*

Actazin™ also provides unique prebiotic activity.Prebiotics are substances that act as food for bacteria inside the gut.Prebiotics are substances that act as food for bacteria inside the gut.Gut bacteria play a major role in maintaining good digestion and overallhealth. Actazin™ has the unique ability to selectively feed bacteriaknown to enhance a healthy gut.* This is an advantage over most otherprebiotics which feed bacteria indiscriminately.prebiotics which feed bacteria indiscriminately.

The Power to Regulate

The fiber in Actazin™ provides bulk and adds moisture to the stool, which helps itmove through the digestive tract more comfortably

The Power to Regulate

move through the digestive tract more comfortably.

Kiwifruit has been reported to have anti-inflammatory effects. Kiwifruit containsPolyphenolics (phytonutrients and antioxidants) Kiwifruit cultivars showed promisingactivity with human THP-1 monocytes and peripheral whole blood.Research suggest that kiwifruit polyphenolics may be useful in diets to help combatResearch suggest that kiwifruit polyphenolics may be useful in diets to help combatthe inflammation underlying many chronic diseases associated with modernlifestyles.

Actazin™ provides all four of these components, and promotes a mild softeningp p p gof the stool in a gentle and sustained manner. Actazin™ has a “normalizing” effecton the bowel, so it can be used long term and will continue to gently support motilitywithout over-stimulation.Even at high doses, it does not cause diarrhea, urgency or cramping. Oftensignificant benefits occur within the first few days of consumption of Actazin™.significant benefits occur within the first few days of consumption of Actazin .

Extensive toxicological and safety testing has shown that Actazin™ is extremelysafe for humans. It is not recommended for those with a known Kiwi allergy.

Actazin is better than a fiber b d l ti

What makes Actazin such an amazing digestive health product, is the

based laxative

synergy from proven enzyme complex in Actazin, which very gentlystimulates motility through the bowel without causing any of the nastyside effects like spasms, cramping and diarrhea that are often

i t d ith tilit d tassociated with motility products.

A study of 53 volunteers confirmed the gentle laxative effects andimproved regularity from kiwifruit WITHOUT the unwanted side effectsimproved regularity from kiwifruit WITHOUT the unwanted side-effectsof gas and bloating normally associated with a fiber based laxative.

Combined with the prebiotic and fiber activity from kiwifruit; ActazinCombined with the prebiotic and fiber activity from kiwifruit; Actazinreally is an outstanding digestive health product.

More than just Kiwifruit

The patented production process protects the bioactives in Kiwifruit and makes

More than just Kiwifruit

the complex of standardised enzymes, prebiotics, polyphenolics and fiber morebioavailable (more available for absorption into the body) than it would be,simply by just eating a whole kiwifruit.

While this Actazin product is made from kiwifruit, people are unlikely get thesame results from just eating the fruit.

A di t i ti t ld h t t i ifi t t f ki if itAccording to scientists, one would have to eat a significant amount of kiwifruitto get similar results.

It would not only just be inconvenient, but it could cause a raft of unpleasanty j , pside effects such as acid build up and an upset stomach.

Market for Gut Health and KiwifruitMarket for Gut Health and Kiwifruit

The gastrointestinal supplements market in the United States isextremely lucrative with the category worth US$819 million in 2006and growing at around 16 per cent per year.

American consumers looking for a natural and proven digestive aidwithout the side effects of gas and bloating.

A Kiwifruit laxative was launched in the New Zealand market in July2007 and by the end of the year had achieved the number oneposition for its category in the pharmacy channel increasing theposition for its category in the pharmacy channel increasing thevalue of that category by nearly 50%.

Dysbiosis – “Out of Balance”Dysbiosis Out of Balance

When the intestines contain the balance of good and bad bacteria that isoptimal for good health, they are described as being in a state of symbiosis.

Alternatively dysbiosis (a contraction of the term “dys-symbiosis ”) occursAlternatively, dysbiosis (a contraction of the term dys symbiosis ) occurswhen this balance is upset. Dysbiosis can result from a deficiency of goodbacteria or an overgrowth of harmful organisms.

S h th it’ f d fi i f d b t i th fSo… whether it’s a case of deficiency of good bacteria or an overgrowth ofa harmful microorganism, any organisms, which are not usually predominantin the intestines, such as unfriendly bacteria, yeast (Candida) and protozoa,can induce disease by altering nutrition patterns in the body.y g p y

Signs and symptoms of dysbiosis

When the digestive system is out of balance, the following symptoms may occur:

Signs and symptoms of dysbiosis

Bloating, belching, burning, flatulence after meals A sense of fullness after eating Indigestion, diarrhea, constipation Systemic reactions after eating Nausea or diarrhea after taking supplements Nausea or diarrhea after taking supplements Rectal itching Weak or cracked finger nails Dilated capillaries in the cheeks and nose in the non-alcoholic Post-adolescent acne or other skin irritations such as rosacea Iron deficiency Chronic intestinal infections, parasites, yeast, unfriendly bacteria Undigested food in the stool Greasy stools Skin that’s easily bruisedSkin that s easily bruised Fatigue Amenorrhea (absence of menstruation) Chronic vaginitis (vaginal irritation)

SPECIFICATION

PRODUCT: Actazin Standardized Kiwifruit

SPECIFICATION

PRODUCT CODE : 21121-00-FSOURCE : New Zealand Only Kiwifruit ( seedless )DESCRIPTION: Free Flowing Fine, Green PowderSHELF LIFE : 3 years in a dry and cool storage environment.

NUTRITIONAL INFORMATION :• energy 325 kcal / 100g• protein 6g / 100g• fat 5g / 100g

b h d t 67 / 100• carbohydrate 67g / 100g• sugars 6g / 100g• sodium 25mg / 100g

Moisture : < 6.0 %T t l A bi Pl t C t (30°C 72 h ) 5 000 fTotal Aerobic Plate Count (30°C -72 hrs) : <5,000 cfuYeast & Moulds : < 100 cfuTotal Coliforms : Not DetectedSalmonella sp. : Absent in 25gParticle Size : 100% < 500 u

Th S iThe Science

Anti-inflammatory effects of kiwifruit

Proceedings of the Nutrition Society of New Zealand, 2008, Volume 32, pages 20-25 Forty Second Annual Conference held in combination with the Australian

Anti inflammatory effects of kiwifruit

25. Forty Second Annual Conference held in combination with the AustralianNutrition Society at Massey University, Albany Campus, Auckland, New Zealand(December 2007).Authors: Farr JM, Hurst SM, Skinner MA.

Abstract: Several foods have been reported to have anti-inflammatoryeffects, including kiwifruit. In this study the ability of natural water extractsof kiwifruit to reduce lipopolysaccharide from pathogenic bacteria; (LPS)-induced tumour necrosis factor (TNF)-α and interleukin (IL)-1β production

as e al ated in itro as a meas re of potential anti inflammator acti itwas evaluated in vitro as a measure of potential anti-inflammatory activity.Kiwifruit cultivars showed promising activity with human THP-1 monocytesand peripheral whole blood, although differential effects were seen inindividual donors.

These results provide supporting evidence for further studies that kiwifruitmay be useful in diets to help combat the inflammation underlying manychronic diseases associated with modern lifestyles.

Kiwifruit: The ability to positively modulate k k f t i t ti l f ti

Proceedings of the Nutrition Society of New Zealand, 2008, Volume 32, pages 20-25. Forty Second Annual Conference held incombination with the Australian Nutrition Society at Massey University, Albany Campus, Auckland, New Zealand (December2007)

key markers of gastrointestinal function.

2007).Authors: Shu Molan AL, Kruger MC, Drummond LN.

Abstract: The consumption of kiwifruit is associated with a number of health benefits related togastrointestinal function. These benefits are generally attributed to the high levels of vitamins,minerals, dietary fibre and bioactive phytochemicals present in kiwifruit. There is a need to, y p y pinvestigate the biological activities of specific fractions of kiwifruit on markers of gastrointestinalhealth.

Aqueous extracts of Kiwifruit and assessed for their ability to influence the growth of bothbeneficial and pathogenic bacteria under in vitro conditions.In addition the potential of ki ifr it to mod late the acti it of ß gl cosidase and ßIn addition, the potential of kiwifruit to modulate the activity of ß-glucosidase and ß-glucuronidase was investigated.

A series of in vitro experiments were conducted to evaluate both the antimicrobial and prebioticproperties, and intestinal bacterial enzyme activities of kiwifruit extracts.In addition extracts prepared from kiwifruit was able to promote the growth of beneficial lacticIn addition, extracts prepared from kiwifruit was able to promote the growth of beneficial lacticacid bacteria (LAB) especially at high concentrations and reduce the growth of Escherichia coli.

It was concluded that kiwifruit extract promotes antimicrobial and prebiotic activities whentested under in vitro conditions. Kiwifruit extract has also been shown to beneficially modulatethe intestinal bacterial

Kiwifruit extract enhances markers of innate d i d i it i d l

Food and Agricultural Immunology 2008; Volume 19, Issue 2 June 2008, pages 149 – 161.Authors: Shu Q, Mendis De Silva U, Chen S, Peng W, Ahmed M, Lu G, Yin Y, Liu

and acquired immunity in a mouse model.

Authors: Shu Q, Mendis De Silva U, Chen S, Peng W, Ahmed M, Lu G, Yin Y, LiuA, Drummond L.

Abstract: The present study examined the effects of a kiwifruit extract on immuneresponse in BALB/c mice. The effects were investigated using cholera vaccine (11days duration) and diphtheria/tetanus toxoid-vaccine (29 days duration) modelsdays duration) and diphtheria/tetanus toxoid-vaccine (29 days duration) models.Mice were given either test (standard diet incorporated with kiwifruit extract) orcontrol diets ad libitum throughout the respective experimental periods. At the end,blood, spleen and intestinal fluids were collected for determination of cellproliferation, specific antibody responses, cytokine production, phagocytosis, and

t l kill ll ti itnatural-killer cell activity.

Kiwifruit extract significantly enhanced (p<0.05) specific intestinal mucosal andserum antibody responses to the vaccines and promoted interferon-gamma andnatural killer cell activities.The overall results of the present study demonstrate the ability of kiwifruit extract toenhance markers of innate and acquired immunity in the tested mouse model.

Presented by:  Grant Washing‐SmithDirector

Anagenix Ltd.