presentation of expression of certain e nzymes in a panel of human cancer c ell lines
DESCRIPTION
Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines. By Sara Soth. Background Information. Adenocarcinoma Cancer Cell Line. P450 Enzymes Antioxidative Enzymes Drug Metabolism (Enzymes) CYP1A1 and CYP1B1 MnSOD Cancer Cell Lines. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/1.jpg)
Presentation of Expression of Certain Enzymes in a Panel of Human Cancer
Cell Lines
By Sara Soth
![Page 2: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/2.jpg)
Background Information
P450 Enzymes Antioxidative Enzymes Drug Metabolism (Enzymes) CYP1A1 and CYP1B1 MnSOD Cancer Cell Lines
Adenocarcinoma Cancer Cell Line
![Page 3: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/3.jpg)
Background Information Continued
P450 Enzymes Includes CYP1A1 and CYP1B1 Catalyze the oxidation of organic
substances.
![Page 4: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/4.jpg)
Antioxidative Enzymes Lack of enzyme causes oxidative
stress, stress will then cause damage or kill cell.
Balance needed.
Background Information Continued
![Page 5: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/5.jpg)
Drug Metabolism (Enzymes)
Equivalent of xenobiotic metabolism. Works with P450 enzymes. Affect on chemotherapy treatment
Background Information Continued
![Page 6: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/6.jpg)
MnSOD Mitochondrial Superoxide Dismutase mRNA Protein
Background Information Continued
![Page 7: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/7.jpg)
Cancer Cell Lines A2780 – Ovarian Cancer
• Carcinoma SKOV-3 – Ovarian Cancer
• Caucasian Adenocarcinoma LOVO and DLD-1– Colorectal Cancer
• Adenocarcinoma MCF-7 and MDA-MB-231– Breast Cancer
• Caucasian Adenocarcinoma HeLa – Cervical Cancer
• Epitheloid Carcinoma C33A – Cervical Cancer
• Carcinoma
Background Information Continued
![Page 8: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/8.jpg)
Methodology RNA isolated by Chomczynski and Sacci
methods. Grown on cell plates Collected at 60% confluent Put in Tris-glycine gel with SDS-page. Transfer to Nitrocellulose Perform immunodetection with Rabbit
polyclonal anti-CYP1B1Ab and MnSOD Ab Densitometric quantification of band
amplification by ImageJ 1.46 software for imaging.
![Page 9: Presentation of Expression of Certain E nzymes in a Panel of Human Cancer C ell Lines](https://reader035.vdocuments.site/reader035/viewer/2022062422/56813319550346895d99ddb1/html5/thumbnails/9.jpg)
Resources http://www.ncbi.nlm.nih.gov/gene/1543 http://www.ncbi.nlm.nih.gov/gene/1545 http://ghr.nlm.nih.gov/gene/CYP1B1 http://
medical-dictionary.thefreedictionary.com/procarcinogen http://www.aafp.org/afp/2007/0801/p391.pdf http://www.merriam-webster.com/medical/immunostaining http://
cancerres.aacrjournals.org/content/57/14/3026.full.pdf http://
www.ncbi.nlm.nih.gov/pmc/articles/PMC2990475/pdf/nihms236168.pdf
http://www.ncbi.nlm.nih.gov/pubmed/20840865 http://www.oxidativestressresource.org/ http://www.ncbi.nlm.nih.gov/pubmed/2440339