S90 Abstracts / Journal of Biotechnology 185S (2014) S37–S125

Preparation of poly(vinyl alcohol) film blendedwith quaternary ammonium chitosannanoparticles loaded tetracycline for use asantibacterial wound dressing

Titiyaporn Tongrain 1,∗, Pitt Supaphol 1, PrasitPavasant 2

1 The Petroleum and Petrochemical College,Chulalongkorn University, Bangkok, Thailand2 Department of Dentistry, ChulalongkornUniversity, Bangkok, Thailand

E-mail address: gentlegirl kik@hotmail.com (T. Tongrain).

Poly(vinyl alcohol) (PVA) films can be prepared by using a solutioncasting method intended for wound dressing application. Hence,the aim of this study was to develop a dressing that can enhancethe wound healing process. The QCh, quaternized derivative of chi-tosan, was synthesized by the quaternization process and exhibitedexcellent water solubility. Water solubility testing, 1H NMR, FT-IR,and Zeta potential measurements were used to confirm the suc-cess of the quaternization. The degree of quaternization of QChwas in a range of 72.0 ± 0.3%, and the positively charged surfacewas about 24–29 mV. The encapsulation of tetracycline (TC) inQCh nanoparticles (QCh NPs) was prepared by ionic gelation. Thesuccess of TC loading into the QCh NPs was confirmed by FT-IR,UV–vis spectrophotometry, and XRD techniques. The QCh/TC NPswere spherical with an average size of 230–800 nm observed bySEM and DLS. The encapsulation efficiency (EE) of TC loaded intothe QCh NPs was about 75–91%, with 2.5–12.5 mg/ml of initialTC. The antibacterial activity of PVA films were studied againstEscherichia coli, Enterococcus faecium, and Staphylococcus aureus.Finally, the indirect cytotoxicity of the wound dressings was stud-ied in mouse fibroblast (L929) and human fibroblast cells by usingMTT assay.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.306

Study on high performance shoes for walkingtraining

Yasuhiro Hayakawa ∗, Kazuma Kanezaki

Department of Control Engineering, Nara NationalCollege of Technology, Nara, Japan

E-mail address: hayakawa@ctrl.nara-k.ac.jp (Y. Hayakawa).

One of the most popular reasons that elderly person fall down inwalking motion is a gap of the center of gravity position of foot.So in order to solve the problem, we propose High-PerformanceShoes that assist elder people to their walking. In this paper weindicate a new system that shows status of walking balance. Thenew system is constructed with High-Performance Shoes, AndroidDevice, Head Mounted Display and Wi-Port. Therefore, we canshow data on real time. We also produce new insole that candetect sole pressure distribution and change insole’s stiffness cor-responded to pressure distribution. Further, we clarified walkingcharacteristics through walking experimental results. By the way,we have to clarify characteristics of shoes quantitatively. How-ever, we cannot evaluate those shoes to use existing evaluationdevice for walk because they cannot reproduce a gap of the cen-ter of gravity position of foot. So we also developed an evaluationdevice for walking that can reproduce a gap of the center of grav-ity position of foot. Finally, we measured what distribution datawere obtained by using evaluation device. Thus we clarified that

we can reproduce human walking repeatedly by using evaluationdevice.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.307

Diagnostic value of electroencephalographicrecording in juvenile myoclonic epilepsy atGerman Shepherd dog

Gabriela Dumitrita Stanciu ∗, Mihai Musteata,Gheorghe Solcan

Department of Clinics, Internal Medicine(Neurology), University of Agricultural Science andVeterinary Medicine “Ion Ionescu de la Brad”, Iasi,Romania

E-mail address: gdstanciu@yahoo.com (G.D. Stanciu).

Background: Juvenile myoclonic epilepsy represents about 7%of the cases of epilepsy in dogs. The syndrome is defined bymyoclonic seizures (jerks) with and without tonic–clonic seizures.The aims of this study were to describe a short time EEG recording(30 min) using Redding’s model to determine the diagnostic valueof electroencephalographic recordings in dogs suffering from juve-nile myoclonic epilepsy. Methods: Electroencephalograms wereperformed on 6 German Shepherds puppies, between 2 and 6months old, suffering from juvenile myoclonic epilepsy. EEGswere obtained via five subdermal needle electrodes, recorded withsensitivity: 70 �V/cm; time constant: 0.3 s; notch filter inserted;impedance of all electrodes <10 k�. Results: Brain electrical activ-ity of all the patients was characterized by the presence of interictalepileptiform discharges. Exclusive presence of single spikes andpolyspikes were recorded in 2 patients (33.33%) and sharp waveand multiple sharp wave complexes were encountered in other 4dogs (66.66%). Conclusion: The interictal epileptiform dischargesdetection proved the presence of an epileptic focus in the absence ofclinical sings, allowing the diagnosis of juvenile myoclonic epilepsyand excluded other non-epileptic movement disorders.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.308

Electroencephalographic changes in dogs’nephrocalcinosis

Paula Maria Saftencu ∗, Gabriela DumitritaStanciu, Mihai Musteata, Sorin Ioan BescheaChiriac, Sorin Aurelian Pasca, Gheorghe Solcan

Department of Clinics, Internal Medicine(Neurology), University of Agricultural Science andVeterinary Medicine “Ion Ionescu de la Brad”, Iasi,Romania

E-mail address: saftencu paula@yahoo.com (P.M. Saftencu).

Background: Nephrocalcinosis is a disorder characterized by thedeposition of calcium salts in the renal parenchyma. Besides thesymptoms of renal failure, the neurological signs are character-ized by seizures which can evolve in to status epilepticus. In suchsituations it is difficult to differentiate clinically the seizures dueto chronic renal failure by those from idiopathic epilepsy (IE).The aim of this study was to describe electroencephalographicchanges in dogs suffering from nephrocalcinosis using a shorttime EEG recording. Methods: EEGs were performed on 5 dogs ofmixed breeds, with ages between 7 and 9 years suffering fromrenal calcinosis proved by specific histological findings. EEGs wereobtained via five subdermal needle electrodes, recorded with sen-sitivity: 70 �V/cm; time constant: 0.3 s; impedance of all electrodes