preimplantation genetic testinng
TRANSCRIPT
Preimplantation Genetic Testing
By
Osama Abdalmageed
Assistant Lecturer of Obstetric & Gynecology, Assiut University,
Assiut, Egypt
Research Fellow, American Center for Reproductive Medicine,
Cleveland, Ohio
Points to be covered in this presentation
Introduction: Overview
History
Important terms
Indications: PGD
PGS
Techniques: Embryo biopsy
Genetic analysis
Challenges: Risks
Legal challenges
Future:
IntroductionHistory-3
1968• Edward and Gardner
• 1st embryo biopsy.
1978• Conventional IVF
• Robert Edward & Patrick Steptoe
1980s• First human PGT for sexing
1990
• Handyside et al
• First child born after PGT (sexing)
2000• PGT is available for most of the known genetic disorders.
Indications
PGD
X-linked disorder: Sex determination
Chromosomal translocation: FISH
Carrier of Autosomal recessive disease: PCR
Carrier of Autosomal dominant disease: PCR
HLA matches:
Indications
PGD/Controversies
Embryo sexing for non-medical:
Testing of non medical indications (eye colour,
hair colour)
HLA for prospective donors:
TechniquesEmbryo biopsy
Weakening of the outer layer of the oocyte or the embryo (Usually laser)
Mechanical extraction of the cell (for genetic testing)
Biopsies
Polar body biopsy
Cleavage stage biopsy
Blastocyst stage biopsy
TechniquesGenetic Analysis
PGD
PCR FISH Microarray
PGS
FISHMicroarray (single
nucleotide polymorphism)
Microarray (comparative genomic
hybridization)
ChallengesRisks
Could > medical conditions.
Physical damage to embryo from biopsy.
Misdiagnosis
Mosaicism. (developing embryos)
Unknown risksBrezina et al; 2012
ChallengesLegal challenges
1- Limit the use of PG testing. (e.g. Germanand Italy)
2- Sexing. (prohibited in Australia, India,China and Tiland).
Reproductive Tourism
PG Testing Future
1- Advances in the genetic testing
detailed evaluation of the humanembryos.
2- PGS evaluation in large studies.