predictingand testing enzymatic activity predicting and testing enzymatic activity charlie harrison...
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![Page 1: Predictingand testing enzymatic activity Predicting and testing enzymatic activity Charlie Harrison Supervisors: Profs. John Ward & Christine Orengo](https://reader035.vdocuments.site/reader035/viewer/2022062517/56649f335503460f94c4f7da/html5/thumbnails/1.jpg)
Predicting and testing enzymatic activity
Charlie HarrisonSupervisors: Profs. John Ward &
Christine Orengo
![Page 2: Predictingand testing enzymatic activity Predicting and testing enzymatic activity Charlie Harrison Supervisors: Profs. John Ward & Christine Orengo](https://reader035.vdocuments.site/reader035/viewer/2022062517/56649f335503460f94c4f7da/html5/thumbnails/2.jpg)
Norcoclaurine
• Benzylisoquinoline alkaloids (BIQAs)– group of plant secondary metabolites
• Includes many interesting compounds• Common entry point: norcoclaurine
(S)-norcoclaurine (R)-norcoclaurine
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Norcoclaurine
• NorcoClaurine Synthase – NCS• Five known examples from three different
species• But BIQAs exist in many more species…• Can we find more NCSs?
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Main Project Aims
• Bioinformatics– Identify uncharacterised proteins that are likely to
exhibit NCS activity• Lab Work– Express these proteins in E.coli and test
predictions
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Bioinformatics
• Available data: sequence and structure
• Use these to predict function
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Bioinformatics
Reproduced from Orengo, Jones & Thornton 2003 with permission
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Bioinformatics
• Structural data is better for identifying functional homologues...
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Bioinformatics
• Structural data is better for identifying functional homologues...but is only available for ~1% of sequences
• Use predictions of structural regions – Gene3D• Starting point: Gene3D family of likely
structural relatives• Sequence clustering
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Bioinformatics
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Lab Work
• Design and order the synthetic genes
Wait 3 weeks…
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Lab Work
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Results
✓✓
✗
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Results
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Thanks