preclinical in vivo imaging solutions
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Preclinical In Vivo Imaging Solutions
S Series
M Series
Z Series
S-0300
S-0650
S-1500
Mini 0/30
Ultra Mini 0
Mini 0/100
Z
Mini Z
Cellular Imaging for Small Animals Redefined
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Deep Brain Functional ImagingHigh spatial and temporal resolution and minimal
invasiveness allow in situ functional imaging in
deep brain structures and kinetic analysis of
neuronal activityCalcium monitoring in the brain at the level of a single neuron
Deep Brain In Situ ImagingExpression in the deep brain can be investigated with
high resolution and minimal invasionCA1 layer of a Thy1-YFP mouse hippocampus
Peripheral Nerve ImagingMinimal invasiveness and high frame rate make
possible in situ screening of transgenic mutant line for
a better lineage maintenanceMotor nerve terminals at the neuromuscular junction in a Thy1-YFP mouse
Angiogenesis QuantificationHigh resolution, surface and depth imaging enable
in situ monitoring of tumor development and Vessel
Detection Software module allows Functional
Capillaries Density accessMicrovasculature and capillaries of the mouse mesentery
Gastrointestinal Tract ImagingEndoscopic access and miniaturized objectives is a
powerful combination to detect and analyze abnormal
cellular pattern through the natural orificesMonitoring of normal colonic crypt after cresyl violet instillation
Stem Cell ImagingHigh resolution and low invasiveness allow stem cell
homing monitoring within the live animalIntramuscularly injected eGFP Mesenchymal Stem Cells
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Laser Scanning Unit
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Cellvizio® LAB Imaging System
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Minimal Invasiveness: 300μm diameter probesHigh Resolution: down to 1.4µmDynamic Cellular Imaging: 200 fps
Introducing a revolutionary solution for in vivo, in situ cellular imaging for small animal
researchers. Cellvizio® LAB is a high speed fluorescence microscope with miniaturized
microprobes capable of high resolution, real time endoscopic imaging of fluorescent tissues,
cells and markers at a micron level. Based on a robust, patented confocal technology which
employs a high resolution fiber bundle, laser scanning unit and sensitive detector optics, the
easy-to-use platform allows real time imaging of cell biology and physiology inside a living
animal.
Minimally invasive because of their diameter size, the Microprobes have applications for
surface, internal and deep imaging of any organ, including brain. Until now these applications
could only be accomplished ex vivo by histological methods or at very low resolution with
whole body imaging techniques. The capability of the system to scan quickly means that
internal biological processes can be witnessed in real time. Intuitive software and image
processing allows for quantitative analysis of biological features and processes.
Provided as a complete solution (with either a 488nm or 660nm excitation laser, Microprobes
and ImageCell Software for easy acquisition and processing) the Cellvizio LAB is a perfect
complement to other preclinical imaging tools, such as high resolution ultrasound, for
the investigation of microscopic/cellular processes in a living animal. With VisualSonics’
experience and reputation in small animal imaging for research, scientists can expect to be
generating useful data quickly, extending the utility of their animal models and advancing
our understanding of biology through never-before-seen views of phenotypic changes at a
microscopic level.
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Stereotaxic Setup
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Complete Imaging Solution
ProFlex®
Endoscopic fiber optic probes provide minimally invasive access to
tissue in vivo and in situ. The probe bundles consist of tens of thousands
of fibers, microscope optics and a proprietary precision connector.
Varying diameters (from 300 μm to 4.2 mm)
Compatible with either 488nm or 660nm Laser Scanning Unit
Auto detection and auto calibration allow rapid interchangeability
Durable, lightweight, flexible to meet the needs in any domain
Laser Scanning Unit
The LSU incorporates sophisticated optics to scan the confocal
plane through the fiber bundle. Sensitive, single-pixel detection of
fluorescence stimulated by the photodiode laser pulse through each
fiber element, combined with the high scan rate allows the study of
dynamic processes in vivo.
Point-scanning confocal microscope technology
Sensitive, single pixel detection
488nm or 660nm excitation configuration
Safe, class 2, photodiode laser
ImageCell™
This software package provides an intuitive, user-friendly
acquisition and processing interface.
Instrument calibration and control
On-the-fly image display and processing
Analytical and quantification tools
Image, video and data export
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ImageCell Software (Standard Suite)
ImageCell provides everything you need to go beyond
imaging and to quantify your results. The software offers
a robust array of analytical and quantification features
via an intuitive, user-friendly interface. The software
allows users to export data, images and videos in a
variety of standard formats.
The ImageCell software package controls:
Image acquisition
Real time display
Post-acquisition processing
On the fly image processing
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Post Acquisition Tools >>
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Advanced Mosaicing Tool
Advanced Mosaicing™ software analyzes image
frames in real time, overlaying and ‘stitching’
successive frames into one, composite image.
This processing interprets the motion of the
ProFlex(TM) microprobe, reducing motion artifact
and enhancing details within the image, and
offers the ability to extend the field of view from
micrometers to centimeters.
Vessel Detection Tool
Allows automated detection and quantification
of capillary diameter, length, density, and area
within a field of view.
Module automatically delineates and segments
the vascular bed
Rapid and relevant measurement of capillaries
Statistical analysis of tumor vascularization
Quantification available: capillary length, area,
diameter, density
Kinetic Analysis Tool
Provides kinetic analysis by quantifying
fluorescence over time.
Changes in intracellular calcium in the hippocampus in response to bicuculline-induced epileptiform activity. A) Time course of the fluorescence intensity of Oregon Green BAPTA-1 in cells shown in (B). Vincent et al. (2006). EMBO 7(11), 1154–1161
Hyperscan Tool
High speed acquisition module that increases
the temporal resolution for imaging dynamic,
subcellular processes (e.g. calcium flux
accompanying neuron activation).
Software and post acquisition tools
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Lymphocytic colitis. Coloflex Z probe. Courtesy of PD. Dr A. Meining, Klinikum rechts der lsar, Munich, Germany
Zoom on the mosaic Corresponding input frame
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Different microprobes for different needs
Visualization of the same
region of interest with
different microprobes
Vascularization of rat sciatic
nerve with nuclear staining:
Acriflavine
FOV: 170 x 120 µm
Vascularization of rat sciatic
nerve with nuclear staining:
Acriflavine
FOV: 440 x 310 µm
Z series probe shown actual size: 1.8mm
M-Series Microprobe S-Series Microprobe
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Optical Microprobes Specifications
*Beveled or flat tip
Tip Diameter
(mm)
Lateral Resolution
(µm)
Optical sectioning
(µm)
Working Distance
(µm)
Max Field Of View
(µm)
0.3 3.3 15 0 300
0.65 3.3 15 0 600 ×
500
1.5 3.3 15 0 600 ×
500
4.2 1.4 10 30 240 ×
200
2.6 1.4 10 60 240
4.2 1.4 10 100 240 ×
200
1.8 3.5 70100/170
at488/660
600 × 500
0.94 3.5 3050/70
at488/660
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Zs e r i e s
DepthImaging
Ms e r i e s
Hi-ResImaging
Ss e r i e s
SurfaceImaging
Brain, deep brain in mice, other organs at depth if low invasiveness is mandatory
Brain, deep brain in rats, other organs if low invasiveness is mandatory
General applicability, can be used to check fluorescence sensitivity in most targets
Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness
Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness
Vessels, angiogenesis, cell fate, cell morphology, utility depends on cell layer thickness and invasiveness
Blood flow through the vessel (without penetration) image deeper cell layers of tumor, organ or tissue
Cavities, eye
S-0650*
S-1500
Mini 0/30
UltraMini 0
Mini0/100
Z
Mini Z
Model
S-0300*
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1010
Imaging
Individual motor nerve terminals of neuromuscular junction of Thy1-YFP mouse.
Interest: Imaging of the peripheral nervous system, and nerve fibers
ProFlex: S-0650, hand-held
Visualized Features: Motor nerve terminals of neuromuscular junction
Field of View: 400 x 280 µm
Courtesy: Igor Charvet, Medical Research Center (CMU), Geneva, Switzerland
and Luc Stoppini, Biocell Interface, Geneva, Switxerland
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Imaging
Neuromuscular JunctionThe neuromuscular junction, where peripheral nerves meet muscle sarcolemma at specialized sites called motor end plates, is an important structure where the etiology of peripheral nerve disorders is concerned.
KidneyThe Cellvizio LAB offers a minimally invasive and therefore longitudinal method for the investigation of small animal nephrology: rapid assessment of the renal microvasculature and vascular permeability, and enables cellular resolution and quantification of molecular biomarkers in vivo in the same animal. (This images shows the kidney glomerulus of a beta-actin GFP mouse.)
LiverThe in vivo evaluation of therapeutics includes biocompatibility, targeting, and toxicity studies which frequently involve investigating the biodistribution, accumulation, and clearance of the therapeutic compounds. The Cellvizio LAB offers the researcher the chance to accurately evaluate these parameters with sub cellular resolution, in situ and in vivo.
LungsThe Cellvizio LAB Imaging System enables the preclinical assessment of functional lung and respiratory tract disease indicators in situ. (This image from a beta-actin GFP mouse shows the micro vessels in the lung alveoli)
Kidney GlomerulusCellvizio LAB enables the imaging and visualization of the vasculature of the Glomerulus, the cluster of capillaries that takes part in the blood filtration and transportation of waste to the urine. (This image is from a beta-actin GFP mouse)
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Support and Training
Scientific SupportThe advanced technology of the Cellvizio LAB
imaging system is supported by an equally
sophisticated approach to service and support.
The VisualSonics team provides expert training
and applications support and is committed to
maintaining system performance.
VisualSonics offers a broad range of service
solutions that meet your needs.
Technical SupportVisualSonics provides on-going Service and
Technical Support with our team of experienced
and certified professionals.
TrainingCustomer Training
Imaging Courses
Symposia – associated with major conferences
Private and Secure
Online Learning Center and
Customer Website
http://www.visualsonics.com
Find publications, protocol guides, imaging
guides & training videos
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VisualSonicsVisualSonics is the undisputed world leader in
real time, in vivo, high-resolution micro imaging
systems, providing modalities specifically
designed for preclinical research.
These cutting edge technologies allow
researchers at the world’s most prestigious
pharmaceutical and biotechnology companies,
hospitals and universities to conduct research
in cardiovascular, cancer, neurobiology
and developmental biology areas. These
technologies support applications that include
genetic research, phenotypic studies and drug
development. VisualSonics platforms combine
high-resolution, real time in vivo imaging
at reasonable cost with ease-of-use and
quantifiable results.
Mauna Kea TechnologiesMauna Kea Technologies, a company with a multi-
disciplinary scientific approach, leads the growing
in vivo cellular imaging market with its flagship
Cellvizio® system and its cellvizio.net interactive
online database.
Cellvizio, the world smallest and most flexible
microscope, provides real time dynamic cellular
images in vivo and in situ. It enables researchers and
physicians in more than 100 prestigious institutions
around the world to raise their practice to a higher
standard of efficiency, accuracy and
cost-effectiveness.
The cellvizio.net scientific knowledge interactive
database is enabling an international community
of leading scientists and physicians to share their
ground-breaking work based on Cellvizio
in gastroenterology and various fields of science
and medicine.
About us
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CORPORATE HEADQUARTERS > 3080 Yonge Street suite 6100
box 66 Toronto Canada M4N 3N1
U.S. >
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New York, NY 10017
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VisualSonics BV
Science Park 406
Matrix V Suite 4.12
1098 XH Amsterdam
The Netherlands
WEBSITE > www.visualsonics.com
TELEPHONE >
+1 416 484-5000
TOLL FREE >
North America
1.866.416.4636
Europe
+ 800.0751.2020
Advancing preclinical research
Cardiovascular
Cancer
Developmental Biology
Diabetes
Neurobiology
Reproductive Biology
Regenerative Medicine
Ophthalmology
Molecular Imaging
Orthopedic
Gene Delivery
Lung
Angiogenesis
Calcium flux imaging
Cell Migration
Apoptosis Quantification
Bone Marrow
Gene Expression
Neuromuscular Junction
VisualSonics™, ™, MicroMarker™,Vevo®, VevoStrain™, DEPO®, SoniGene™ are trademarks of VisualSonics Inc.
ProFlex®, Confocal Miniprobe®, Cellvizio®, ImageCell™, HyperScan™, Advanced Mosaicing™, Vessel Detection™ are trademarks of Mauna Kea Technologies
© 2009 VisualSonics Inc. All rights reserved. Version 1.0.