Pre-Lab of Reflected Light Microscopy

A. Describe each of the following properties below AND determine how they are observed in a reflecting microscope (10 pts each)

1) Color

2) Reflectivity/Reflectance

3) Hardness

4) Cleavage

5) Bireflectance

6) Anisotropism

7) Internal Reflections

• Incomplete answers get an automatic 0

B. Compare and contrast reflected light and transmitted light microscopy (10 pts)

C. Draw a sketch of one of the new microscopes in the laboratory (Rm 115). Label the following parts and identify its function or use.

1) Light source

2) Filter

3) Aperture control

4) Field of view control

5) Oculars

6) Analyzer

7) Polarizer

8) Rotating nosepiece

9) Objectives

10) Stage

11) Stage lock

12) Stage centering screw

13) Stage adjustment knob

14) Fine-adjustment knob

15) Coarse-adjustment knob

16) On/Off switch

17) Base

18) Body

19) Vernier scale

Reminders

• No sketch yields a maximum grade of 30/100 pts