L. Venkatachalam, R.V. Sreedhar and N. Bhagyalakshmi The use of genetic markers for detecting DNA polymorphism, genotype identification and phylogenetic relationships among banana cultivarsRakeshkumar S. Choudhary 2009-11-102

Genetic variations and relationships among 21 banana cultivars of South India were evaluated using 50 decamer RAPD primers and 12 ISSR primersThe RAPD and ISSR surveys between pairs of 21 cultivars revealed 60.15% and 56.73% of polymorphic bands, respectivelyBased on these data, a genetic similarity matrix was established and a dendrogram for each set of primers was developed by UPGMAThe study resulted in the identification and molecular classification of South Indian banana cultivarsA b s t r a c t

KeywordsBananaGenetic diversityRAPDISSRResolving power Jaccards similarity coefficient

India has been considered as one of the important centres of diversity for bananaThe diverse agroclimatic conditions have encouraged the development and sustenance of a large number of varieties catering to local needsBanana belongs to the genus Musa L. (Family Musaceae, order Gingiberales) Musa acuminata Colla (contributes for A genome) and Musa balbisiana Colla (contributes for B genome)Genetic diversity in plants can be estimated in several ways here by RAPD and ISSR marker

Introduction

IntroductionPresent evaluation involves commercially important diploid, triploid and tetraploid cultivars, the information generated is expected to be of great help in finding genetic relationships and planning breeding programmes

Table

Materials and methods1. Plant material2. Genomic DNA extraction3. Selection of primers and band profile reproducibility4. RAPD and ISSR analyses

Materials and methods5. PCR amplification The protocol for RAPD analysis The protocol for ISSR analysis6. Resolving power (Rp)7. Data analysis

RESULTS Selection of primers and reproducibility Molecular analysis and fingerprinting of banana cultivars

RAPD profile of DNA from 21 cultivars 0f banana primers OPA-14 (A), OPN-06 (B)

RAPD profile of DNA from 21 cultivars 0f banana using primers OPM-20 (C) and OPC-05 (D)

ISSR profile of DNA from 21 cultivars of South Indian banana cultivars using primer UBC-810. Lane Marker 100 bp Gene Ruler DNA ladder

Discussion Fingerprinting and cultivar-specific markers Genetic relationships Comparison of RAPD and ISSR markers

Dendrogram displaying the genetic distances among banana cultivars obtained from cluster analysis of RAPD data.

Dendrogram displaying the genetic distances among banana cultivars obtained from cluster analysis of ISSR data.

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