Plant Tissue Culture is culturing of any part of the plant in a specially defined growth media under aseptic laboratory condition in Petri dishes, test tubes or in any other suitable glass containers.Banana plants produced from Tissue Culture are free from diseases at the time of supply and they give high yields. India is endowed with tropical climate with abundant solar energy throughout the year, which favours growing crops round the year.

There is a vast scope to increase irrigation potential by river projects and minor irrigation projects.

In additional to the above, India is blessed with more labourer availability.

Since agriculture is the primary sector. Total geographical area : 328.848 million. Total reporting area : 304.300 million. Area under cultivation : 143.000 million. Total cropped area : 179.750 million . Area sown more than once : 36.750 million. Area not available for cultivation :

161.300million. Area under forest : 66.400 million .

Realizing the importance of shoot meristem culture in getting rid of the plant disease causing pathogens and its subsequent mass propagation. Berg and Bustamante (1974) attempted technique. However, this procedure is not completed effectively in the absence of suitable diagnosis. Soon after reports of Banana plantlets produced by invitro shoot tip culture came from Taiwan (Ma and Shill, 1974).

Production of large number of plant lets within a short time less labor costs Large scale and commercially

viable technologies were developed

Large number of uniform propagules can be generated in a relatively short period of time.

Variability encountered in size and propagules density especially in clones suckering erratically can be minimized.

It could allow for rapid bulking of novel clones when used in concert with breeding programms.

Successful micro-propagation strategies are as a result of interplay of plant material, the culture milieu and the

micro-environment.

SHOOTING

ROOTING

Primary hardening will take atleast 4 weeks depending upon the climatic conditions. In final week these trays are gradually exposed to 50% shade by removing plastic sheets. These plantlets are sprayed with fungicides, bactericide, and water soluble fertilizers as per schedule.

Is a separate air conditioned Dust free room Equipped with the requisite number of

laminar airflow A high level of cleanliness ensures An ethanol dip, an alcohol lamp/

Bunsen/ burner/incinerator/hot bead sterilizer etc.

Ashwini Parab 32 Manish Kalshetty 15 Amol Jadhav 11 Tejas kamble 18 Ajay pawar 35 Kiran Dolas 09