ribosome

• Bacterial 30S (small) subunit

• 16S rRNA (orange)

• 21 proteins (blue)

ribosome

• Bacterial 50S (small) subunit

• 26S rRNA (orange)

• proteins (blue)

(from Wikipedia)

• Eukaryotes have 80S ribosomes, each consisting of a small (40S) and large (60S) subunit. Their 40S subunit has an 18S RNA (1900 nucleotides) and 33 proteins. The large subunit is composed of a 5S RNA (120 nucleotides), 28S RNA (4700 nucleotides), a 5.8S RNA (160 nucleotides) subunits and 46 proteins.

• blast your SPAdes assembly against known rDNA sequence

• Use grep –A 1 to pull out that sequence from the assembly

• Download to your computer

• Blast against NCBI to see what it hits, confirm it is what we want

• blast your SPAdes assembly against known rDNA sequence

• Use grep –A 1 to pull out that sequence from the assembly

• blast your SPAdes assembly against known mitochondrial sequence

• Use grep –A 1 to pull out that sequence from the assembly

• Download each sequence to your computer

• Blast against NCBI to see what it hits, confirm it is what we want