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✓ Be able to kill the organism in question

✓ Achieve good and complete distribution

✓ Achieve thorough and total penetration

✓ Achieve sufficient contact time

at the correct concentration

5 6

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✓ Be able to kill the organism in question

? Achieve good and complete distribution

? Achieve thorough and total penetration

? Achieve sufficient contact time

at the correct concentration

Chlorine Dioxide Gas

Registration Sterilant

• Pre-Conditioning Raise relative humidity to 65-75%

• ConditioningHold that humidity level for a short period of time

• Charge Inject CD Gas to a concentration of 1 - 5 mg/L

• Exposure Dwell time at that CD Gas concentration

• Aeration Remove CD Gas

Target Organism Dosage Required using CD Gas

Salmonella ~100 ppm-hrs for 6-log kill

Listeria ~300 ppm-hrs for a 5-log kill

Spores ~600 ppm-hrs for 6-log kill

ClorDiSys Decon Cycle 720 ppm-hrs

RH Dosage Required for 6-log Spore Reduction

65% ~600 ppm-hrs

55% ~1000 ppm-hrs

45% ~1550 ppm-hrs

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13

Chlorine Dioxide Gas

Boiling Point °

Natural State at Room Temperature

18

All the treatments were for 10 min at 20oC

0

1

2

3

4

5

6

7

8

9

Log R

eductions

Uninjured Surface

Injured Surface

Water

Washing

CD Gas CD Liquid

(submerged)

Aa

Ac

By

Bx Ab

By

(Han, Y. et al, Reduction of Listeria monocytogenes on Green Peppers (Capsicum annuum L.) by Gaseous and

Aqueoous Chlorine Dioxide and Water Washing and Its Growth at 7oC, Journal of Food Protection, Vol 64, No 11,

2001 pages 1730-1738)

Equal concentrations of CD Gas and Liquid used

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QA Method Description

Concentration Monitor Verifies the concentration of CD gas during a decontamination and overall cycle dosage

Biological Indicators Verifies that the decontamination cycle achieved 6-log sporicidal reduction

Swab Testing … you know this one.

Chlorine Dioxide Gas

Color Yellow-Green

Concentration Monitoring

uv-vis spectrophotometer

Chlorine Dioxide Gas

Concentration Monitoring

Highly Accurate

Injection Locations

Sensor Locations20

10

10

6

2 7

9

1

5

4

3

8

19 17

18 16

15

13 11

12

14

1

2

3

4

5

6

7

8

9

10

20

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25 26

27 28

Chlorine Dioxide Gas

Temperature Not a factor above 52 F

Starting Relative Humidity Not a factor

Equipment Within Space Not a factor, gas gets everywhere

Room Shape / Size Not a factor, gas gets everywhere

Injection Point Location Not a factor, gas gets everywhere

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37

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S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 S12 S13 S14 S15 S16 S17 S18 S19 S20 S21 S22 S23 S24 S25 S26

9:45 AM 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

10:00 AM 0.04 0.04 0.07 0.14 0.11 0.14 0.14 0.22 0.14 0.25 0.25 0.18 0.25 0.18 0.29 0.18 0.22 0.25 0.25 0.33 0.25 0.25 0.22 0.25 0.29 0

10:15 AM 0.18 0.25 0.33 0.29 0.18 0.29 0.22 0.36 0.44 0.44 0.25 0.33 0.36 0.33 0.36 0.33 0.33 0.36 0.36 0.44 0.36 0.36 0.36 0.33 0.4 0.36

10:30 AM 0.29 0.33 0.36 0.44 0.25 0.4 0.33 0.47 0.58 0.69 0.4 0.47 0.55 0.51 0.51 0.44 0.51 0.4 0.4 0.47 0.44 0.44 0.44 0.4 0.44 0.4

10:45 AM 0.36 0.44 0.51 0.51 0.29 0.44 0.4 0.55 0.77 0.8 0.4 0.51 0.62 0.58 0.58 0.55 0.58 0.47 0.47 0.58 0.47 0.55 0.51 0.51 0.55 0.44

11:00 AM 0.44 0.47 0.58 0.58 0.33 0.51 0.44 0.62 0.84 0.95 0.47 0.58 0.73 0.69 0.66 0.66 0.69 0.58 0.58 0.62 0.55 0.62 0.55 0.55 0.62 0.51

11:15 AM 0.51 0.55 0.62 0.62 0.36 0.55 0.51 0.66 0.95 1.03 0.47 0.62 0.77 0.73 0.73 0.69 0.73 0.62 0.62 0.69 0.55 0.66 0.62 0.58 0.73 0.55

11:30 AM 0.55 0.62 0.73 0.73 0.44 0.62 0.62 0.77 1.03 1.18 0.55 0.66 0.84 0.73 0.8 0.8 0.84 0.69 0.69 0.77 0.66 0.73 0.66 0.62 0.73 0.58

11:45 AM 0.62 0.69 0.8 0.77 0.47 0.69 0.66 0.8 1.14 1.25 0.62 0.73 0.92 0.88 0.88 0.88 0.94 0.77 0.69 0.84 0.73 0.77 0.73 0.69 0.84 0.66

12:00PM 0.62 0.69 0.8 0.77 0.47 0.69 0.66 0.8 1.14 1.25 0.62 0.8 1.03 0.95 0.95 0.88 0.84 0.95 0.77 0.84 0.73 0.77 0.73 0.69 0.84 0.66

12:15 PM 0.69 0.73 0.84 0.84 0.55 0.77 0.69 0.88 1.21 1.36 0.66 0.8 1.03 0.99 0.95 0.92 0.92 0 0.95 0.88 0.73 0.84 0.8 0.77 0.92 0.66

12:30 PM 0.73 0.8 0.92 0.88 0.55 0.77 0.73 0.95 1.25 1.36 0.66 0.8 1.06 1.03 0.95 0.95 0.95 0.95 0.86 0.92 0.73 0.88 0.8 0.8 0.95 0.73

12:45 PM 0.8 0.84 0.95 0.95 0.62 0.84 0.77 0.95 1.33 1.44 0.77 0.95 1.06 1.06 0.99 0.99 0.99 1.06 0.95 0.95 0.8 0.92 0.88 0.84 0.95 0.77

1:00 PM 0.8 0.92 0.99 0.99 0.58 0.88 0.8 1.03 1.4 1.44 0.69 0.95 1.14 1.14 1.03 1.03 1.03 1.06 0.88 0.95 0.8 0.92 0.88 0.84 0.99 0.77

1:15 PM 0.88 0.95 1.03 0.99 0.58 0.88 0.8 1.06 1.4 1.44 0.73 0.95 1.18 1.21 1.1 1.1 1.03 1.1 0.92 1.03 0.88 0.95 0.92 0.84 1.06 0.81

1:30 PM 0.88 0.95 0.99 0.95 0.58 0.8 0.73 1.1 1.33 1.4 0.73 0.95 1.18 1.21 1.06 1.14 1.06 1.14 0.95 1.03 0.88 0.95 0.92 0.8 0.99 0.8

1:45 PM 0.88 0.92 0.95 0.95 0.58 0.84 0.77 1.03 1.29 1.33 0.77 0.02 1.14 1.18 1.03 1.06 1.03 1.03 0.84 0.95 0.88 0.92 0.88 0.77 0.95 0.8

2:00 PM 0.8 0.84 0.88 0.92 0.62 0.88 0.8 0.99 1.21 1.29 0.77 0.99 1.1 1.1 0.99 0.99 0.99 1.06 0.84 0.95 0.88 0.92 0.84 0.8 0.92 0.84

2:15 PM 0.77 0.8 0.88 0.88 0.62 0.88 0.8 0.88 1.21 1.21 0.73 0.95 1.06 1.06 0.95 0.95 0.95 0.95 0.73 0.92 0.84 0.88 0.84 0.8 0.92 0.84

2:30 PM 0.7 0.7 0.75 0.8 0.6 0.84 0.78 0.84 1.18 1.18 0.7 0.92 1.02 1 0.9 0.9 0.9 0.9 0.7 0.82 0.78 0.8 0.8 0.7 0.8 0.78

2:45 PM 0.62 0.66 0.69 0.73 0.58 0.8 0.77 0.8 1.14 1.18 0.69 0.92 0.99 0.99 0.84 0.84 0.84 0.88 0.69 0.77 0.73 0.77 0.73 0.66 0.73 0.73

3:00 PM 0.51 0.55 0.66 0.69 0.47 0.69 0.69 0.69 1.03 1.1 0.47 0.77 0.88 0.92 0.69 0.77 0.73 0.73 0.55 0.7 0.58 0.66 0.62 0.58 0.73 0.51

3:15 PM 0.49 0.53 0.55 0.6 0.4 0.52 0.5 0.6 0.96 1.04 0.4 0.6 0.8 0.8 0.6 0.6 0.66 0.68 0.47 0.69 0.5 0.55 0.55 0.47 0.69 0.47

3:30 PM 0.47 0.51 0.51 0.51 0.33 0.47 0.4 0.58 0.92 0.99 0.33 0.55 0.73 0.73 0.55 0.47 0.58 0.62 0.4 0.47 0.4 0.49 0.51 0.4 0.6 0.4

3:45 PM 0.33 0.35 0.37 0.35 0.25 0.35 0.31 0.48 0.78 0.82 0.25 0.49 0.61 0.61 0.48 0.35 0.47 0.55 0.31 0.4 0.31 0.42 0.37 0.33 0.51 0.33

4:00 PM 0.24 0.28 0.31 0.29 0.2 0.27 0.24 0.4 0.64 0.68 0.18 0.42 0.5 0.5 0.4 0.27 0.4 0.51 0.24 0.33 0.22 0.35 0.31 0.25 0.35 0.24

4:15 PM 0.17 0.2 0.24 0.21 0.13 0.2 0.18 0.31 0.52 0.55 0.11 0.35 0.43 0.43 0.31 0.2 0.33 0.37 0.18 0.25 0.13 0.29 0.24 0.2 0.29 0.17

4:30 PM 0.11 0.13 0.18 0.14 0.08 0.13 0.11 0.22 0.37 0.39 0.06 0.29 0.35 0.36 0.22 0.13 0.25 0.31 0.11 0.18 0.08 0.23 0.18 0.13 0.21 0.11

4:45 PM 0.07 0.1 0.11 0.09 0.03 0.07 0.6 0.13 0.25 0.3 0.03 0.23 0.27 0.27 0.13 0.07 0.2 0.24 0.6 0.11 0.03 0.15 0.11 0.08 0.14 0.07

5:00 PM 0.03 0.05 0.06 0.03 0 0.02 0.01 0.08 0.18 0.21 0 0.15 0.17 0.16 0.08 0.02 0.13 0.18 0.01 0.06 0 0.09 0.06 0.03 0.09 0.03

5:15 PM 0 0.01 0.01 0 0 0 0 0.03 0.11 0.14 0 0.09 0.11 0.11 0.03 0 0.08 0.11 0 0.03 0 0.05 0.01 0 0.03 0

5:30 PM 0 0 0 0 0 0 0 0 0.06 0.08 0 0.05 0.04 0.04 0 0 0.03 0.06 0 0 0 0.01 0 0 0 0

5:45 PM 0 0 0 0 0 0 0 0 0.03 0.04 0 0.01 0 0 0 0 0 0.01 0 0 0 0 0 0 0 0

6:00 PM 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Total PPM-Hrs 1312 1431 1590 1587 1012 1460 1391 1735 2414 2592 1238 1627 2062 2023 1803 1724 1610 1822 1530 1690 1432 1637 1536 1413 1733 1351

S11 S12

9:45 AM 0 010:00 AM 0.25 0.1810:15 AM 0.25 0.3310:30 AM 0.4 0.4710:45 AM 0.4 0.5111:00 AM 0.47 0.5811:15 AM 0.47 0.6211:30 AM 0.55 0.6611:45 AM 0.62 0.7312:00PM 0.62 0.812:15 PM 0.66 0.812:30 PM 0.66 0.812:45 PM 0.77 0.951:00 PM 0.69 0.951:15 PM 0.73 0.951:30 PM 0.73 0.951:45 PM 0.77 0.022:00 PM 0.77 0.992:15 PM 0.73 0.952:30 PM 0.7 0.922:45 PM 0.69 0.923:00 PM 0.47 0.773:15 PM 0.4 0.63:30 PM 0.33 0.553:45 PM 0.25 0.494:00 PM 0.18 0.424:15 PM 0.11 0.354:30 PM 0.06 0.294:45 PM 0.03 0.235:00 PM 0 0.155:15 PM 0 0.095:30 PM 0 0.055:45 PM 0 0.016:00 PM 0 0

Total

PPM-Hrs 1238 1627

Concentration Monitoring Data

✓ Be able to kill the organism in question

✓ Achieve good and complete distribution

✓ Achieve thorough and total penetration

✓ Achieve sufficient contact time

at the correct concentration