PowerPlex® Fusion 6C SystemPerformance Packed.

Performance Packed.

60 100 200 300 400 50065 1246

9MA

*A = Amelogenin

D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E

D16S539 D18S51 D2S1338 CSF1PO Penta D

TH01 vWA D21S11 D7S820 D5S818 TPOX

D8S1179 D12S391 D19S433 SE33 D22S1045

DYS391 FGA DYS576 DYS570

A*

Figure 1. Configuration of the PowerPlex® Fusion 6C System. The PowerPlex® Fusion 6C System allows co-amplification and fluorescent detection of 27 loci, including all CODIS and ESS loci: D3S1358, D1S1656, D2S441, D10S1248, D13S317, D16S539, D18S51, D2S1338, CSF1PO, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, SE33, D22S1045, FGA and DYS391, as well as DYS576, DYS570, Penta E, Penta D and Amelogenin.

This six-color system allows co-amplification and fluorescent detection of the new 20 CODIS Core Loci: CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, FGA, TH01, TPOX, vWA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433 and D22S1045 (1) plus Amelogenin and DYS391 for gender discrimination. Penta D, Penta E and SE33 also are included to increase discrimination and allow searching of databases that contain profiles with these loci. Eleven loci are less than 250bp, making them ideal for working with degraded samples. Finally, two rapidly mutating Y-STR loci, DYS570 and DYS576, are included in the multiplex to help determine the number of contributors in a mixture.

The PowerPlex® Fusion 6C System offers rapid-cycling, direct-amplification capabilities and superior sensitivity along with SE33 and three Y-STR loci for improved mixture interpretation and unbeatable performance with challenging casework samples.

Additionally, the system is optimized and QC-tested to work at full- or half-volume reactions with some protocol modifications recommended depending on sample type.

1. Hares, D.R. (2015) Selection and implementation of expanded CODIS core loci in the United States. Forensic Sci. Int. Genet. 17, 33–4.

Enhanced SensitivityThe PowerPlex® Fusion 6C System reliably produces complete profiles from as little as 125pg of human DNA and useable, partial profiles from DNA inputs lower than 125pg.

Superior Inhibitor ToleranceCasework samples are typically laden with inhibitors and can often require laboratories to re-amplify samples and repeat analyses. The PowerPlex® Fusion 6C System contains a robust buffer system to meet those challenges head on and increases your chances for success with even the most difficult samples.

Figure 2. The average peak heights (vertical bars) and percentage of alleles called (diamonds) at varying amounts of 2800M Control DNA. Error bars represent the standard deviation of the mean from triplicate amplification reactions.

Figure 3. Resistance to PCR inhibitors. Human genomic DNA (1ng) was amplified with the indicated kits in the presence of common PCR inhibitors. Amplified products were separated on an Applied Biosystem® 3500 Genetic Analyzer (1.2kV, 15-second injection). Percentage of alleles detected is indicated for each system.

1314

1MA

25,000

20,000

15,000

5,000

02ng 1ng 500pg 250pg 125pg 62.5pg 31.2pg

10,000

120

100

80

40

20

0

60

Peak

Hei

ght (

RFU)

Input DNA Amount

Alleles Called (%)

PowerPlex® Fusion 6C System

GlobalFiler® PCR Amplification Kit

Investigator® 24Plex QS Kit

1256

0MA

PowerPlex® Fusion 6C System GlobalFiler® PCR Amplification Kit

Alle

les

Calle

d (%

)

Hematin Humic Acid Tannic Acid

120

100

80

60

40

20

0500µM 750µM 1,000µM 100ng/µl 200ng/µl 300ng/µl 300ng/µl 400ng/µl100ng/µl

Direct-Amplification CapabilitiesThe PowerPlex® Fusion 6C System allows direct amplification from unwashed FTA® card punches as well as pretreated nonFTA card punches and typically used swabs to streamline workflows and expand your laboratory’s sample throughput.

To maximize the cost effectiveness of STR systems, many laboratories incorporate the use of half-volume (12.5μl) amplification reactions in their workflow. The PowerPlex® Fusion 6C system is designed and QC-tested to work at full- or half-volume reactions with various protocol modifications recommended depending on sample type.

PowerPlex® Fusion 6C System

1114

0MC

Punch FTA® card into plate

Punch card into plate; treat with PunchSolution™

Reagent

Incubate swab in SwabSolution™

Reagent

NonFTA Punches SwabsFTA® Punches

Add PCR amplification

mix to dried punch

Add 2µl of extract to PCR amplification

mix

Cycling time ~65 minutes for all applications

Add PCRamplification

mix

Extract DNAfrom sample

Extracted DNA

Add up to 15µlof sample to

reaction

FTA® Punch Analysis

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2MA

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D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E

D16S539 D18S51 D2S1338 CSF1PO Penta D

TH01 vWA D21S11 D7S820 D5S818 TPOX

D8S1179 D12S391 D19S433 SE33 D22S1045

DYS391 FGA DYS576 DYS570

A…

Figure 4. Direct amplification of two 1.2mm punches from a buccal sample on an FTA® card. Two 1.2mm punches were added to a plate, and PCR amplification mix was added to the punches following the recommended protocol for 12.5µl reactions. Amplified products were separated using an Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).

Buccal Sample on FTA® Card

Buccal Swab Analysis

1256

3MA

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

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50 100 150 200 250 300 350 400 450 500

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A… D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E

D16S539 D18S51 D2S1338 CSF1PO Penta D

TH01 vWA D21S11 D7S820 D5S818 TPOX

D8S1179 D12S391 D19S433 SE33 D22S1045

DYS391 FGA DYS576 DYS570

Figure 5. Direct amplification of DNA from buccal swabs. A buccal swab was pretreated with SwabSolution™ Reagent as recommended in the SwabSolution™ Kit Technical Manual. Following incubation, 2µl of extract was added to the PCR amplification mix using the recommended protocol for 12.5µl reactions. Amplified products were separated using an Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).

Swab Sample

PowerPlex® Fusion 6C System

Product InformationThe PowerPlex® Fusion 6C System is compatible with the Applied Biosystems® 3500 and 3500xL Genetic Analyzers as well as Applied Biosystems® 3130 and 3130xl Genetic Analyzers with Data Collection Software Version 4.0 with the DC v4 6-Dye Module v1 License (Life Technologies).

Each PowerPlex® Fusion 6C System contains:

• PowerPlex® Fusion 6C 5X Master Mix• PowerPlex® Fusion 6C 5X Primer Pair Mix• 2800M Control DNA, 10ng/μl• Water, Amplification Grade• PowerPlex® Fusion 6C Allelic Ladder Mix • WEN Internal Lane Standard 500

For more information, visit: www.promega.com/PowerPlexFusion

NonFTA® Punch Analysis

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

1314

4MA

0

7000

14000

0

7000

14000

0

7000

14000

0

7000

14000

0

7000

14000

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

50 100 150 200 250 300 350 400 450 500

D3S1358 D1S1656 D2S441 D10S1248 D13S317 Penta E

D16S539 D18S51 D2S1338 CSF1PO Penta D

TH01 vWA D21S11 D7S820 D5S818 TPOX

D8S1179 D12S391 D19S433 SE33 D22S1045

DYS391 FGA DYS576 DYS570

A…Figure 6. Direct amplification of a single 1.2mm punch from a buccal sample collected using the Bode Buccal DNA Collector™ device. A punch was pretreated with PunchSolution™ Reagent as recommended in the PunchSolution™ Technical Manual. Following incubation, PCR amplification mix was added to the dried punch following the recommended protocol for 12.5µl reactions. Amplified products were separated using an Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).

Buccal Sample on Bode Buccal DNA Collector™ Device

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Ordering Information

Product Size Cat.#

PowerPlex® Fusion 6C System 50 (or 100 direct-amp) reactions DC2705

200 (or 400 direct-amp) reactions DC2720

PowerPlex® 6C Matrix Standard 5 preps DG4900

PunchSolution™ Kit 100 preps DC9271

SwabSolution™ Kit 100 preps DC8271

5X AmpSolution™ Reagent 100 preps DM1231

PowerPlex® Fusion 6C System

Maxwell, Plexor and PowerPlex are registered trademarks of Promega Corporation. AmpSolution, Differex, DNA IQ, Identity Automation, PowerQuant, PunchSolution, Slicprep and SwabSolution are trademarks of Promega Corporation.

Applied Biosystems is a registered trademark of Applied Biosystems. Bode Buccal DNA Collector is a trademark of the Bode Technology Group, Inc. FTA is a registered trademark of Flinders Technologies, Pty, Ltd., and is licensed to Whatman.GlobalFiler is a registered trademark of Life Technologies. Investigator is a registered trademark of Qiagen GmbH Corp.

Products may be covered by pending or issued patents or may have certain limitations. Please visit our Web site for more information.