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Potential of LNEG and EERA-Bioenergy partners for contributing for joint cooperation with Brazil within LCE-22:

Sub-challenge c)

Francisco GírioHead of Bioenergy

LNEG - National Laboratory of Energy and Geology, Portugal

Coordinator of the Sub-Programme “Biomass Biochemical Conversion to Advanced Biofuels & Biomaterials”

EERA-Bioenergy

Brazil-EU Workshop: Coordinated Call on Advanced Lignocellulosic

Biofuels – FAPESP-São Paulo, Brazil, December 8th, 2015

Development of new fermentation and separation technologies for advanced liquid biofuels and applied research to increase the energy efficiency of advanced biofuel processes

The National Laboratory for Energy and Geology, I.P.

(LNEG) is a State R&D institution belonging to the

Ministery of Environment, Territory Planning and

Energy:

i) Laboratory of Energy (LEN)

ii) Laboratory of Geology and Mines (LGM)

R&DManagement

NP EN

ISO 17025UNE-EN

ISO 9001:2008

NP 4457:2007

Logotype on:

HR Excellence

in Research

(European

Charter of

Researchers)

HRs

National Laboratory of Energy and Geology, (Lisbon, Portugal)

Quality

An unique B+B Research Infrastructure in Portugal

BBRI - Biomass and Bioenergy Research InfrastructureNational Roadmap of Research Infrastructures (2014-2020)

Head: Francisco Gírio, PhD

P1. BIOCHEMICAL PLATFORM

P2. MICROALGAE PLATFORM

P3. THERMOCHEMICAL PLATFORM

P4. ANALYTICAL SCIENCES FOR BIOFUELS

P5. BIOFUELS AND BIOMASS SUSTAINABILITY

Research Facilities on:

Staff= 30 researchers + 40 grantees and students.

Project title

ProEthanol2G

Integration of Biology and Engineering into

an Economical and Energy-Efficient 2G Bioethanol Biorefinery

Call (part) identifier

FP7-ENERGY-2009-BRAZIL

THEME [ENERGY.2009.3.2.3]

[Second Generation Biofuels – EU-Brazil Coordinated Call]

Starting date Duration

November 1st, 2010 – Oct 30th, 2014 - 48 months -

4th International Conference on Lignocellulosic EthanolLANDSHUT-MUNICH | 23-25 SEPTEMBER 2014

ProEthanol2G Project | Partners

LNEG, PORTUGAL (EU COORDINATOR)

LABORATÓRIO NACIONAL DE ENERGIA E GEOLOGIA I.P.

DTU, DENMARK

DANMARKS TEKNISKE UNIVERSITET

ULUND, SWEDEN

LUNDS UNIVERSITET

CIEMAT, SPAIN

CENTRO DE INVESTIGACIONES ENERGETICAS MEDIOAMBIENTALES Y TECNOLOGICAS

INBICON, DENMARK

INBICON A/S

FRAUNHOFER, GERMANY

FRAUNHOFER GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E. V.

UGent, BELGIUM

UNIVERSITEIT GENT

GV, SWITZERLAND

GREENVALUE SA

EPFL, SWITZERLAND

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE

HOLM, DENMARK

HOLM CHRISTENSEN BIOSYSTEMER APSBIOSYSTEMER

SELECTED R&D LNEG PROJECTS P1. BIOCHEMICAL PLATFORM

TO ADVANCED BIOFUELS

Convénio FCT-CAPES 2014-2015:

Transport and XOS fermentation in

Saccharomyces cerevisiae for

improvement the advanced bioetanol

production from lignocellulose

Beta-xylosidase activity

Cooperation with UFMG, Brasil

BIOBLOCKS – Creation of bio-based

products as precursors for the bio-

manufacturing of chemical synthesis

and biomaterials (2013-2015).

Call: H2020-LCE-11-2015

Type of action: RIA

Proposal number: 654365-2

Proposal acronym: BABET-REAL5 (2016-2020)

Call: H2020-ERA NET-LAC

Type of action: RIA

Proposal number:

Proposal acronym: SMIBIO (2015-2018)

LNEG role (partner): To develop robust C5/C6 yeast strains

LNEG role (coordinator): To develop economically feasiblesmall-scale biofuel biorefineries

Recently H2020 funded projects

P2. MICROALGAE PLATFORM

TO ADVANCED BIOFUELS

ADVANCED BIOFUELS FOR AVIATION:

Mixed Thermochemical + Biochemical

conversion of microalgae biomass

DEMA- Direct Ethanol from MicroAlgae (7thFP,

2013-2017)

SELECTED R&D LNEG PROJECTS

MICROALGAE

WW-SIP - From Urban

Wastewater Treatment

Plant to Self-

Sustainable Integrated

Platform for

Wastewater

Refinement

(LIFE, 2012-2015)

GASBIOREF - Gasification of Biofuels

and Recovered Fuels – 7thFP (2010-

2014)

P3. THERMOCHEMICAL

PLATFORM TO ADVANCED

BIOFUELS

PTDC/AAG-REC/3477/2012–

RICEVALOR – Energetic valorization

of wastes obtained during rice

production in Portugal – FCT (2013-

2015)

PTDC/AGR-FOR/3872/2012–

ENERSTUMPS – Undersurface

Biomass from Eucalyptus globulus –

FCT (2013-2015)

SELECTED R&D LNEG PROJECTS

ADVANCED BIOFUELS FOR AVIATION:

Mixed Thermochemical + Biochemical

conversion of microalgae biomass

P4. ANALYTICAL SCIENCES FOR

BIOFUELSSELECTED R&D LNEG PROJECTS

CHARACTERIZATION AND COMPLIANCE ASSESSMENT

Gaseous Biofuels - Biogas, Biomethane

Liquid biofuels - Biodiesel, diesel/biodiesel blends,

bioethanol, jet biofuels,…

Solid Biomass - biofuels, waste-derived fuels (RDF)

Liquid fuels - diesel and petrol, oils and fuel oils ...

Solid fuel - coal, ...

RXLEN - Identification of crystalline compounds

and other elements of inlays deposits in electric

power plants by WD-XRF (T&TA)in partnership with EDP, for identification of waste deposits

and ash sources, in scattered spots on plants

CHARACTERIZATION AND COMPLIANCE ASSESSMENT

Control of impurities in lignocellulosic-based feedstocks after

pretreatments

Characterization of phenolics, heavy metals, etc in

lignocellulosic materials

P5. SUSTAINABILITY OF

BIOFUELS AND BIOMASS SELECTED R&D LNEG PROJECTS

Advanced Bioethanol production flowsheet from

Sugarcane bagasse and trash (Proethanol2G)

(1a) Trash harvesting from field , loading and transportation

(2) Biomass Pretreatment

(4) Hydrolysis

(5) C5/C6 Fermentation

Sep. 3 S/L

(7) Distillation and dehydration

HP steam

(9) Steam and power generation

C5 stream

(3) Enzyme production

Bioethanol

Biogas (8) WWT/Anaer. digestor

Propagation

Recomb. yeast

Sep. 2 S/L

S

L

Sep. 1 S/L

Lignincake

S

L S

(1) Sugarcane bagasse from 1G

plant

Electricity

Water

(4) Mixing tank

(1b) Trash cleaning and milling

Bagasse

Clear mash

S

L

Process simulation using SuperPro Designer

LCIA using SimaPro (and

Ecoinvent databases)

The main challenges of today´sadvanced biofuels projects in Europeare:

Competitiveness with eithergasoline/diesel or with 1G (~technology issues)

Price, logistics and availability of largescale biomass sustainable supply

LCE-22-2016: International Cooperation with Brazil on advanced lignocellulosic

biofuels

Call sub-challenges that should be addressed by the proposals:

a. Gasification of bagasse to syngas and advanced liquid fuel production, including biofuels for aviation.

b. Applied research to biomass production logistics and applied research for feedstock diversification for advanced biofuels.

c. Development of new fermentation and separation technologies for advanced liquid biofuels and applied research to increase the energy efficiency of advanced biofuel processes.

Scope: Moving technologies from TRL 3-4 to TRL 4-5

“This means: focus on research for

technological breakthroughs!”

Biochemical Value Chains #5 AND #6 for Advanced Biofuels (EU SET PLAN)

SP2


Lignocellulosic ethanol

BIOMASS PretreatmentCellulose and Hemicellulose

Hydrocarbons from sugars (biochemical)

Enzymatic Hydrolysis

Glucose, Xylose, others

Fermentation Ethanol

BIOMASS Pretreatment SugarsYeast/Bacterial Fermentation

Hydrocarbons Hydrogenation

Synthetic diesel and/or jet-type

biofuels

Hydrocarbons from sugars (chemical)

BIOMASS PretreatmentSugars,

HMFCondensation/ Hydrogenation

Alkanes for jet and diesel

engines

Lignocellulosic higher alcohols and novel biofuel molecules

BIOMASS PretreatmentEnzymatic Hydrolysis Fermentation Ethanol

Chemical synthesis

Alkanes or its precursors for jet and diesel

engines

Biochemistry Conversion Processes


Novel biofuel conversion routes (from LC sugars)

Necessary to drastically increase energy (pathways) efficiencies*

Rude and Schirmer (2009) Curr Op Microbiol 12, 274-281.


* Other R&D issue: to overcome high toxicity levels of some of new molecules

Biochemistry Conversion Processes to Advanced biofuels


R&D key challenges:

Process technologies compatible with utilization of multi-flexible feedstocks

Development of flexible (and less energy-intensive) integrated pretreatment with simultaneous separation of contaminants/inhibitors

Reduce fermentation complexity (one pot vs two pots) and improve biological performance through maximization of LC sugars utilization

Use of synthetic biology for novel and robust industrial cell factories platforms

Integrate adequately in a “biorefinery context” lignin and side streams

Simultaneously, improve the value chain sustainability through craddle-to-gate GHG reductions

Smarter Integration biochemical/chemical technologies

SP2 - Biochemical processing of biomass for advanced biofuels and biomaterials

List of current R&D participants

Our SP2 Network: 23 participants and associates (from 12 EU countries)

LNEG, Coordinator - Portugal

BERA (WARE), including KU Leuven (Belgium)

CIEMAT, CENER, IMDEA, IREC, TECNALIA, CTAER (Spain)

ENEA, CNR, Univ. Perugia, Univ. Padova (Italy)

ECN, WUR (The Netherlands)

INRA, CNRS (France)

DTU, Aalborg Univ. (Denmark)

SINTEF (Norway)

UKERC, e.g., U. Bangor, U. Aberystwyth, University of Cambridge (UK)

Lund Univ., SP (Sweden)

VTT (Finland)

CUT, IFFMPAS (Poland)

EERA bioenergy SP2 partners and expertise:

PRETREATMENTS + EH

AALBORG UNIV (hydrothermal, mechanical)CIEMAT/CENER (steam explosion, high-solids loading,

enzymatic technology)CNR (acid & alkaline, enzymatic technology)ECN (organosolv) ENEA (steam explosion, organolsolv) LNEG (hydrothermal, green solvents, ionic liquids, high

pressure mixtures CO2/Water, enzymatic technology) SP/UNIV. LUND (process technology; enzyme technology)WUR (green biorefineries; protein removal)


www.eera-bioenergy.eu

Innovative biomass pretreatmentswith green solventes or Sc CO2

towards advanced biofuels(current TRL 2-3)

CO2-assisted

hydrothermal pre-

treatment

Glucan and lignin

-rich solid

C5-sugar-rich liquor

Direct source of

oligosaccharides(up to 60% more XOS than in

autohydrolysis)

Furfural

(advanced fuel precursor)(yield of 70% and selectivity and conversion of C5

sugars > 80%)

Lignin depolymerisation to

value-added chemicals

@ T∽120°C

Imid

azo

le

mAU

0

5

10

15

20

25

30

35

40

Rosmarinic ac. 90%

nm

Rosmarinic acid

Vanillin 86%

nm

Vanillin

Vanillic ac. 90%

nm

Vanilic acid

min4 6 8 10

Ab

s(3

20

nm

)

0

25

50

75

100

0 24 48 72 96

Glu

can

to

glu

cose

yie

ld (

%)

Enzymatic hydrolysis time (h)

225⁰C 54 bar Autohydrolysis Untreated

higher alcohols

(advanced fuels)

Contact: Francisco Gírio

LC BIOMASS

Edited by: Rafal Lukasik (LNEG)

Biorefinery at the ENEA research center, Italy Steam explosion and fractionaction to Hem/Cell/Lignin 300 kg/h pilot facility. Internal view

Hydrolysiszone

Fermentation

Bioreactors with innovative design

Pretreatment and fractionation of lignocellulosics in biphasic system and moderate severity

Energy recovery from residues of biorefinery (lignin and other) by gasification: Fixed bed pilot plant and bench reactor water supercritical

Main scientific partner of the BIOCHEMTEX in setting up the 2G EtOH plant of Crescentino

for 75.000 m3/h

Upgrading of syngas to ultrapure hydrogen andH2/CO mix for FT synthesis

Contact: Francesco Zimbardi

Contact: Mercedes Ballesteros

Steam explosion pretreatment plant

2-L laboratory prototype for LHW pretreatment

Continuous twin screw extruder

CIEMAT Technology Challenges for EH:- To find the way of reducing enzyme loading w/o yield loss- To increase the enzymatic cocktail efficiency- To develop high consistency EH to achieve high sugars concentration

EERA-Bioenergy SP2 partners and expertise:

MICROBIAL PLATFORMS (incl. synthetic biology)

AALBORG UNIV. (yeast platforms) IMDEA (yeast platforms)INSA/INRA (yeast platforms)KU LEUVEN (yeast platforms) LNEG (yeast and bacterial platforms)LUND UNIV. (yeast platforms, enzyme technology,

bacterial platform for isoprene pathway)WUR (fermentation & separation technologies)



0

1

2

3

4

5

6

7

0 24 48 72

Co

nce

ntr

atio

n

Time (h)

T18 HAA1* 1gDW/L

Glucose g%

Xylose g%

Glycerol g%

Acetic Acid g%

Ethanol %v/v

Lab of Molecular Cell Biology

KU Leuven

Department of Molecular

Microbiology

VIB

• Powerful polygenic analysis

platform for complex traits in

yeast

• Targeted improvement of

complex traits in industrial yeast

strains by specific allele

exchange

• Development of powerful 2G

bioethanol strains

• Development of cell factory yeast

strains for production of green

chemicals with 1G and 2G

substrates

Efficient cofermentation of glucose and xylose in

undetoxified, concentrated bagasse hydrolysates

Research & Development

Rio de Janeiro

Business Development& Service to Industry

Leuven

Developing and delivering superior

industrial yeast strains for the biobased

economy

Contact: Johan Thevelein

C5 Fermentation: reducing xylitol

production

Site-directed Mutagenesis Alternative sources of XR

Bengtsson et al (2009). Biotechnol. Biofuels.2(1):9; Runquist etal (2010). Appl. Environ Microbiol. 76:7796-802

Contact: Marie Françoise Gorwa-Grauslund

LUND

UNIVERSITY

Contact: Michael O´Donohue

Partial CBP strategy

• Yeast are well-established industrial workhorses

– Cellulolytic yeast are rare

• Yarrowia lipolytica produces lipids (~ 0.40g/g glucose)

– But does not produce detectable b-glucosidase activity

– Does harbor putative b-glucosidase genes

• Achievements so far:– Homologous overexpression of Bgl1

and Bg2

– Growth tests on cellodextrins

– Growth tests on cellulose in presence of cocktails (Cellic CT2)

Bgl2

Increased tolerance

to inhibitors

Efficient co-

fermentations of

different kind of

sugars

Robust yeast cells

for processes at high

gravity. Understand

the mechanical

stress on cells

S. cerevisiae

K. marxianus

Enzyme

production

Production of high

value products from

cheap and abundant

raw materials

Ethanol production at

high temperature from

diverse raw materials

IMPROVING LIGNOCELLULOSIC

SUGARS UTILIZATION

NEW CELL FACTORIES

EFFICIENT YEAST FOR

BIOETHANOL PRODUCTION

Contact: Elia Tomás

New fermentation and separation technology for advanced biofuels (ABE, Biobutanol, Jet fuel)

“In-situ” product removal to relieve product toxicity

Increased Productivity and Sugar utilisation

Energy efficient product recovery Adsorption

Integrated process results in >50% energy savings

Fermentor and stripper

1. Base Case

2. Adsorption

Case


Contact: Richard Gosselink


CHEMICAL PLATFORM (for biofuels)

CNRS (chemical catalysis, green fuels)INRA (hemicellulose & lignin platforms)LNEG (hemicellulose platform)LUND UNIV. (hemicellulose & lignin platforms)WUR (lignin upgrading to high added value products)



High-throughput Evaluation of catalytic activity, HT catalysts synthesis

Catalytic reactions : from lab to pilot scale (UPCAT)

Advanced facilities in surface analyses: XPS, TOF-SIMS, LEIS, EXAFS, operando

Screening of the catalysts for biorefineries (REALCAT platform)

From biomass to biofuels and highly valued chemicals (FDCA, Adipic Acid, Maleic

acid…), furan compounds transformation, glycerol valorization,

Fischer-Tropsch, H2 from biomass

Hybrid catalysis for biomass valorization

Creation of the ‘MATCAT’ team 1st January 2016 for materials synthesis

Biorefinery, green fuels, hybrid catalysis, DeNOx-VOC, biomass valorization

UCCS CNRS 8181Contact: Robert Wojcieszak/Franck Dumeignil


SUSTAINABILITY ASSESSMENT (for biomass/biofuels)

AALBORG UNIV (LCA, cost-benefit analysis)CIEMAT (socioeconomic, environmental analysis and

externalities)ENEA (modelling, GaBI, ASPEN, CHEMCAD)INRA (biomass sustainability, cost-supply)LNEG (modelling: SuperPro Designer; LCA: SimaPro,

Ecoinvent, GaBI)



30

ENER

GY

SY

STEM

S A

NA

LYSI

S U

NIT

Environmental Analysis and Externalities• Life Cycle Sustainability Assessment applied to energytechnologies• Quantification of greenhouse gas emissions of biofuels anddevelopment of biofuel GHG emissions calculation tools in order toimplement the RED in Spain.• Application of the ExternE methodology to energy systemsexternalities assessment.

Socioeconomic assessment

• Application of the Input-Output methodology to estimate directand indirect socioeconomic impacts of energy technologies.

•Development of integrated tools for analysis of the three pillars ofsustainability of energy technologies including environmental,economic and social aspects using E&S-MRIO

• Energy policies assessment using Cost Benefit Analysis.

• Analysis of policy instruments aimed at fostering renewableenergies as well as renewable energy cooperation at theinternational level

•Analysis of the effects that renewable energy investments may havein the development process of less developed countries (LDC)

Energy Modelling• Prospective and back casting analysis of the

national/regional/global energy system at medium and long term

• Analysis of the influence of economic or/and policy measures

Contact: Yolanda Lechón

www.lneg.ptwww.eera-bioenergy.eu

Contact: Francisco Gí[email protected]

Many ThanksEU Consortium (in talks):

LNEGKU Leuven, Global Yeast NV….

Brazil Consortium (in talks):

Univ. San Carlos (SP)2 Brazilian companies….

potential of lneg and eera-bioenergy partners for ... · lneg (hydrothermal, green solvents, ionic...

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