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PORTUGAL

The Report referred to in Article 9 of Directive 2003/99/EC

TRENDS AND SOURCES OF ZOONOSES ANDZOONOTIC AGENTSIN HUMANS, FOODSTUFFS, ANIMALS ANDFEEDINGSTUFFS

including information on foodborne outbreaks,antimicrobial resistance in zoonotic agents and somepathogenic microbiological agents.

IN 2013

Portugal - 2013 Report on trends and sources of zoonoses

INFORMATION ON THE REPORTING AND MONITORING SYSTEM

Country:

Reporting Year:

Portugal

2013

Laboratory name Description Contribution

INIAV - Instituto Nacionalde Investigação Agrária eVeterinária

National Institute of Agricultural andVeterinary Research

Data on zoonoses and zoonotic agentsin food anda animals

DGAVDirecção Geral deAlimentação e Veterinária

National Veterinary Authority Reporting Authority Co-ordination of report production

INSAInstituto Nacional deSaude Dr. Ricardo Jorge

Reference laboratory belonging to theMinistry of Health

Data on zoonoses and zoonotic agentsin humans and foodborne outbreaks

ASAEAutoridade de SegurançaAlimentar e Económica

National Authority for Food Safety Data on zoonoses and zoonotic agentsin food

IBCPInstituto BacteriológicoCâmara Pestana

Scientific Institute - National ReferenceLaboratory for Rabies

DGS - Direcção Geral deSaude National Authority for Human Health Data on zoonoses and zoonotic agents

in humans and foodborne outbreaks

R.A. MADEIRARegião Autónoma daMadeira

Regional Veterinary Services Madeira Data on zoonoses and zoonotic agentsin food and animals

R.A. AçoresRegião Autónoma dosAçores

Regional Veterinary Services Azores Data on zoonoses and zoonotic agentsin food and animals

Laboratorio de Viseu Regional Veterinary Laboratory Data on zoonoses and zoonotic agentsin food and animals

FMV - Faculdade deMedicina Veterinaria Veterinary School in Lisbon Data on zoonoses and zoonotic agents

in animals


INFORMATION ON THE REPORTING AND MONITORING SYSTEM

Laboratory name Description Contribution

UTAD - Universidade deTrás-os-Montes e AltoDouro

Veterinary School in Vila Real Data on zoonoses and zoonotic agentsin food

IPIMARInstituto das Pescas daInvestigação e do Mar

National Veterinary Laboratory Data on zoonoses and zoonotic agentsin food and animals

Portugal - 2013


PREFACEThis report is submitted to the European Commission in accordance with Article 9 of CouncilDirective 2003/99/ EC*. The information has also been forwarded to the European Food SafetyAuthority (EFSA).

The report contains information on trends and sources of zoonoses and zoonotic agents in

The information covers the occurrence of these diseases and agents in humans, animals,foodstuffs and in some cases also in feedingstuffs. In addition the report includes data onantimicrobial resistance in some zoonotic agents and commensal bacteria as well asinformation on epidemiological investigations of foodborne outbreaks. Complementary data onsusceptible animal populations in the country is also given. The information given covers bothzoonoses that are important for the public health in the whole European Community as well aszoonoses, which are relevant on the basis of the national epidemiological situation.The report describes the monitoring systems in place and the prevention and control strategiesapplied in the country. For some zoonoses this monitoring is based on legal requirements laiddown by the Community Legislation, while for the other zoonoses national approaches areapplied.The report presents the results of the examinations carried out in the reporting year. A nationalevaluation of the epidemiological situation, with special reference to trends and sources ofzoonotic infections, is given. Whenever possible, the relevance of findings in foodstuffs andanimals to zoonoses cases in humans is evaluated.The information covered by this report is used in the annual Community Summary Report onzoonoses that is published each year by EFSA.

Portugal during the year 2013 .

* Directive 2003/ 99/ EC of the European Parliament and of the Council of 12 December 2003on the monitoring of zoonoses and zoonotic agents, amending Decision 90/ 424/ EEC andrepealing Council Directive 92/ 117/ EEC, OJ L 325, 17.11.2003, p. 31

Portugal - 2013


List of Contents

1 ANIMAL POPULATIONS 12 INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS 6

72.1 SALMONELLOSIS72.1.1 General evaluation of the national situation92.1.2 Salmonellosis in humans

132.1.3 Salmonella in foodstuffs412.1.4 Salmonella in animals592.1.5 Salmonella in feedingstuffs632.1.6 Antimicrobial resistance in Salmonella isolates

1202.2 CAMPYLOBACTERIOSIS1202.2.1 General evaluation of the national situation1212.2.2 Campylobacteriosis in humans1252.2.3 Campylobacter in foodstuffs1292.2.4 Antimicrobial resistance in Campylobacter isolates1502.3 LISTERIOSIS1502.3.1 General evaluation of the national situation1512.3.2 Listeriosis in humans1532.3.3 Listeria in foodstuffs1702.4 E. COLI INFECTIONS1702.4.1 General evaluation of the national situation1722.4.2 E. coli infections in humans1752.4.3 Escherichia coli, pathogenic in foodstuffs1772.5 TUBERCULOSIS, MYCOBACTERIAL DISEASES1772.5.1 General evaluation of the national situation1772.5.2 Tuberculosis, mycobacterial diseases in humans1802.5.3 Mycobacterium in animals1872.6 BRUCELLOSIS1872.6.1 General evaluation of the national situation1882.6.2 Brucellosis in humans1892.6.3 Brucella in foodstuffs1912.6.4 Brucella in animals2062.7 YERSINIOSIS2062.7.1 General evaluation of the national situation2072.7.2 Yersiniosis in humans2092.8 TRICHINELLOSIS2092.8.1 General evaluation of the national situation2092.8.2 Trichinellosis in humans2122.8.3 Trichinella in animals2162.9 ECHINOCOCCOSIS2162.9.1 General evaluation of the national situation

Portugal - 2013


2172.9.2 Echinococcosis in humans2202.10 TOXOPLASMOSIS2202.10.1 General evaluation of the national situation2212.10.2 Toxoplasmosis in humans2242.11 RABIES2242.11.1 General evaluation of the national situation2252.11.2 Lyssavirus (rabies) in animals2272.12 STAPHYLOCOCCUS INFECTION2272.12.1 General evaluation of the national situation2272.13 Q-FEVER2272.13.1 General evaluation of the national situation2282.14 WEST NILE VIRUS INFECTIONS2282.14.1 General evaluation of the national situation

3 INFORMATION ON SPECIFIC INDICATORS OF ANTIMICROBIAL 2292303.1 ESCHERICHIA COLI, NON-PATHOGENIC2303.1.1 General evaluation of the national situation2303.1.2 Escherichia coli, non-pathogenic in foodstuffs2313.1.3 Antimicrobial resistance in Escherichia coli, non-pathogenic2373.2 ENTEROCOCCUS, NON-PATHOGENIC2373.2.1 General evaluation of the national situation2373.2.2 Antimicrobial resistance in Enterococcus, non-pathogenic isolates

4 INFORMATION ON SPECIFIC MICROBIOLOGICAL AGENTS 2442454.1 CRONOBACTER2454.1.1 General evaluation of the national situation2454.1.2 Cronobacter in foodstuffs2464.2 HISTAMINE2464.2.1 General evaluation of the national situation2464.2.2 Histamine in foodstuffs2484.3 STAPHYLOCOCCAL ENTEROTOXINS2484.3.1 General evaluation of the national situation2484.3.2 Staphylococcal enterotoxins in foodstuffs

5 FOODBORNE OUTBREAKS 251


1. ANIMAL POPULATIONS

The relevance of the findings on zoonoses and zoonotic agents has to be related to the size andnature of the animal population in the country.

1Portugal - 2013


Sources of informationDGAV - Direcção Geral de Alimentação e Veterinária

A. Information on susceptible animal population

2Portugal - 2013

3


Portugal - 2013

Table Susceptible animal populations

30155 984670meat production animals

7876 418565dairy cows and heifers

38480 459963calves (under 1 year)

20658mixed herds

50813 372253 1480549

Cattle (bovine animals)

- Unknown

57Deer farmed

13 3123410 714972Ducks - Unknown

188 2917244 8907872laying hens

1495 180511520 32029182broilers

117 1307874 4443587

Gallus gallus (fowl)

parent breeding flocks,unspecified

18Geese - Unknown


32546animals under 1 year

Goats

Number of herds or flocks Number of slaughteredanimals

Livestock numbers (liveanimals) Number of holdings

Animal species Category of animals Data Year* Data Year* Data Year* Data Year*

* Only if different than current reporting year

4


Portugal - 2013


1700 94171milk goats

6987mixed herds

12561 122350 316505

Goats

- Unknown

206889breeding animals

1711013fattening pigs

200838breeding animals -unspecified - sows and gilts

4781 1920911 - Unknown

4303498unspecified

917632

Pigs

fattening pigs - unspecified -piglets


356599animals under 1 year (lambs)

2486 312988milk ewes

6987mixed herds

25313 868980 1999999

Sheep

- Unknown




5


Portugal - 2013


3216 3126 50791Solipeds, domestic horses

156 3426007 2151490Turkeys - Unknown

14Wild boars farmed

764Guinea fowl - Unknown

8587058Quails - Unknown

5246893Rabbits farmed

27Ratites (ostrich, emu,nandu) farmed





2. INFORMATION ON SPECIFIC ZOONOSES AND ZOONOTIC AGENTS

Zoonoses are diseases or infections, which are naturally transmissible directly or indirectlybetween animals and humans. Foodstuffs serve often as vehicles of zoonotic infections.Zoonotic agents cover viruses, bacteria, fungi, parasites or other biological entities that arelikely to cause zoonoses.

6Portugal - 2013


2.1 SALMONELLOSIS

2.1.1 General evaluation of the national situation

National evaluation of the recent situation, the trends and sources of infectionSalmonellosis in animals (other than Gallus gallus):The animals are sampled on a voluntary basis. The data come from sick animals sent to laboratory forbacteriological analysis or to control herds.There is a Control Programme for Gallus gallus (breeding flocks). There is also going a baseline study onthe prevalence of salmonella in broilers (finished).Control measures are been taken in positive flocks of laying hens.There is also going on a baseline study (started on 2007), on the prevalence of salmonella in slaugherpigsand turkeys.

Additional informationDiagnostic techniques:Foodstuffs/Feedingstuffs - Screening: VIDAS SLM (AFNOR validation). Confirmation: ISO 6579 (2002).

Serology: Rapid Plate Agglutination for S.pullorum/gallinarum.

Bacteriology: ISO 6579 (2002) and D Annex. - Pre-enrichment in Buffered Peptone Water (for faeces, bedding, nests samples and fluffy)- Selective enrichment in MSRV (modified semisolid Rappaport Vassiliadis) and Rappaport Vassiliadiswith Soja broth.- Plating on solid media XLD and SM2 Agar.- Biochemical reactions by 32E or API 20E strips.

Typing of Salmonella: Serotyping by Kauffman/White technique (searching of O antigens by plateagglutination and H antigens by tube agglutination).Serotyping of isolates is performed at LaboratÃ³rio Nacional de InvestigaÃ§Ã£o VeterinÃ¡ria (NRL).

Phagetyping for Salmonella Enteritidis and Salmonella Typhimurium has started on January 1999, seedata on the tables (not in routine analyses).

Antimicrobial Susceptability testing of Salmonella: Resistance to antimicrobials is performed atLaboratÃ³rio Nacional de InvestigaÃ§Ã£o VeterinÃ¡ria ( NRL for Salmonella )

Â·The resistance to antimicrobials is performed by disk diffusion Method in Mueller Hinton Plates.

Â·The antimicrobials tested are: AMP10, AMC30, CF30, CMX30, CTX30, SxT25, G10, K30, TE30, C30,S10, NA30, UB30, N30, D30, ENR5 .

Â·The zone diameters are evaluated, following NCCLS Vol.19 nÂº1, January 99.

A. General evaluation

7Portugal - 2013


2.1.2 Salmonellosis in humans

Reporting system in place for the human casesEstimaded coverage 0.5%

Case definitionCase is considered when laboratory criteria is confirmed.

Diagnostic/analytical methods usedSerotyping and PFGE

Notification system in placeNotifiable disease

History of the disease and/or infection in the countryEndemic

Relevance as zoonotic diseaseMajor

A. Salmonellosis in humans

9Portugal - 2013

10


Portugal - 2013

Table Salmonella in humans - Species/serotype distribution

42 0 42 0 0 0 0S. Enteritidis

35 0 35 0 0 0 0S. Typhimurium

Cases Cases Inc. Autochthon cases

Autochthon Inc.

Importedcases

ImportedInc.

Unknownstatus

Species/serotype Distribution

Salmonella 77 0 77 0 0 0 0

11


Portugal - 2013

Table Salmonella in humans - Age distribution

0 0 0 0 0 0 4 2 2<1 year

12 6 6 8 6 2 37 20 171 to 4 years

15 8 7 5 3 1 29 16 125 to 14 years

1 0 1 0 0 0 5 1 415 to 24 years

3 2 1 0 0 0 8 6 225 to 44 years

0 0 0 2 1 1 9 5 445 to 64 years

1 0 1 2 1 1 12 7 565 years and older

10 3 0 18 6 1 50 15 8Age unknown

42 19 16 35 17 6 154 72 54Total :

S. Enteritidis S. Typhimurium Salmonella spp.Age distribution

All M F All M F All M F

12


Portugal - 2013

Table Salmonella in humans - Seasonal distribution

2 3 15January

0 0 1February

0 1 6March

1 2 8April

9 1 15May

1 3 4June

2 2 8July

3 4 13August

5 1 15September

6 4 24October

5 12 26November

8 2 19December

0 0 0not known

42 35 154Total :

S.Enteritidis

S.Typhimuri

um

Salmonella spp.

Seasonal Distribution

Months Cases Cases Cases

13


Portugal - 2013

2.1.3 Salmonella in foodstuffs

Table Salmonella in poultry meat and products thereof

DGAV Objectivesampling

Officialsampling

food sample> meat Domestic Single 25 Gram 39 0Meat from broilers (Gallus gallus) - fresh -

Processing plant - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 25 0

Meat from broilers (Gallus gallus) - meat preparation- intended to be eaten cooked - Processing plant -Surveillance

ASAE Objectivesampling

Officialsampling food sample Domestic Batch 25 Gram 85 2

Meat from broilers (Gallus gallus) - meat preparation- intended to be eaten cooked - Retail - Surveillance


Officialsampling


Meat from broilers (Gallus gallus) - meat products -cooked, ready-to-eat - Processing plant -Surveillance



Meat from broilers (Gallus gallus) - minced meat -intended to be eaten cooked - Retail - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 8 0Meat from turkey - fresh - Processing plant -

Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 11 0Meat from turkey - meat products - cooked, ready-to

-eat - Processing plant - Surveillance


Officialsampling


Meat from turkey - meat products - raw but intendedto be eaten cooked - Processing plant - Surveillance

INSA Objectivesampling

Officialsampling

food sample> meat Unknown Batch 25 Gram 140 29 10Meat from broilers (Gallus gallus) - fresh - Border

inspection activities - Surveillance

Source ofinformation

Samplingstrategy Sampler Sample type Sample origin Sampling unit Sample

weight Units testedTotal unitspositive forSalmonella

S. EnteritidisS.

Typhimurium

14


Portugal - 2013



Officialsampling food sample Domestic Batch 25 Gram 45 0Meat from broilers (Gallus gallus) - fresh - chilled -

Retail - Surveillance



Meat from broilers (Gallus gallus) - meat preparation- intended to be eaten cooked - frozen - Retail -Surveillance


Officialsampling food sample Domestic Single 25 Gram 2 0

Meat from broilers (Gallus gallus) - meat products -cooked, ready-to-eat - chilled - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 5 4 4Meat from duck - fresh - chilled - Retail -

Surveillance


Officialsampling food sample Domestic Batch 25 Gram 5 4 4Meat from other poultry species - carcase - chilled -




Meat from other poultry species - meat preparation -intended to be eaten cooked - chilled - Retail -Surveillance


Officialsampling food sample Domestic Batch 25 Gram 25 0Meat from turkey - fresh - chilled - Retail -

Surveillance


Officialsampling


Meat from turkey - meat preparation - intended tobe eaten cooked - Processing plant - Surveillance



Meat from turkey - meat preparation - intended tobe eaten cooked - chilled - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 50 0Meat from turkey - meat products - cooked, ready-to

-eat - frozen - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 10 0Meat from turkey - minced meat - intended to be

eaten cooked - chilled - Retail - Surveillance




S. EnteritidisS.

Typhimurium

15


Portugal - 2013


Meat from broilers (Gallus gallus) - fresh -Processing plant - Surveillance


2Meat from broilers (Gallus gallus) - meat preparation- intended to be eaten cooked - Retail - Surveillance

1Meat from broilers (Gallus gallus) - meat products -cooked, ready-to-eat - Processing plant -Surveillance

Meat from broilers (Gallus gallus) - minced meat -intended to be eaten cooked - Retail - Surveillance

Meat from turkey - fresh - Processing plant -Surveillance

Meat from turkey - meat products - cooked, ready-to-eat - Processing plant - Surveillance

Meat from turkey - meat products - raw but intendedto be eaten cooked - Processing plant - Surveillance

1 9 9Meat from broilers (Gallus gallus) - fresh - Borderinspection activities - Surveillance

Meat from broilers (Gallus gallus) - fresh - chilled -Retail - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecifiedS. 4,5,12:i:- S. Anatum S. Derby S. Heidelberg S. Kedougou S. Minnesota

16


Portugal - 2013


4Meat from broilers (Gallus gallus) - meat preparation- intended to be eaten cooked - frozen - Retail -Surveillance

Meat from broilers (Gallus gallus) - meat products -cooked, ready-to-eat - chilled - Retail - Surveillance

Meat from duck - fresh - chilled - Retail -Surveillance

Meat from other poultry species - carcase - chilled -Retail - Surveillance

Meat from other poultry species - meat preparation -intended to be eaten cooked - chilled - Retail -Surveillance

Meat from turkey - fresh - chilled - Retail -Surveillance


1Meat from turkey - meat preparation - intended tobe eaten cooked - chilled - Retail - Surveillance

Meat from turkey - meat products - cooked, ready-to-eat - frozen - Retail - Surveillance

Meat from turkey - minced meat - intended to beeaten cooked - chilled - Retail - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecifiedS. 4,5,12:i:- S. Anatum S. Derby S. Heidelberg S. Kedougou S. Minnesota

17


Portugal - 2013


18


Portugal - 2013

Table Salmonella in milk and dairy products



Cheeses made from goats' milk - soft and semi-soft -made from raw or low heat-treated milk - Retail -Surveillance


Officialsampling

food sample> milk Domestic Single 25 Gram 48 0

Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk -Processing plant - Surveillance



Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk - Retail- Surveillance


Officialsampling


Cheeses made from cows' milk - fresh - made frompasteurised milk - Processing plant - Surveillance


Officialsampling


Cheeses made from cows' milk - soft and semi-soft -made from pasteurised milk - Processing plant -Surveillance



Cheeses made from cows' milk - soft and semi-soft -made from pasteurised milk - Retail - Surveillance


Officialsampling


Cheeses made from goats' milk - fresh - made frompasteurised milk - Processing plant - Surveillance


Officialsampling


Cheeses made from goats' milk - hard - made fromraw or low heat-treated milk - Processing plant -Surveillance



Cheeses made from goats' milk - hard - made fromraw or low heat-treated milk - Retail - Surveillance




S. EnteritidisS.

Typhimurium

19


Portugal - 2013



Officialsampling


Cheeses made from goats' milk - soft and semi-soft -made from pasteurised milk - Processing plant -Surveillance


Officialsampling

food sample> milk Domestic Single 25 Gram 18 0Cheeses made from sheep's milk - fresh -




Cheeses made from sheep's milk - hard - made fromraw or low heat-treated milk - Retail - Surveillance


Officialsampling


Cheeses made from sheep's milk - soft and semi-soft - made from pasteurised milk - Processing plant- Surveillance



Cheeses made from sheep's milk - unspecified -made from raw or low heat-treated milk - Retail -Surveillance


Officialsampling


Cheeses, made from mixed milk from cows, sheepand/or goats - fresh - made from pasteurised milk -Processing plant - Surveillance


Officialsampling


Cheeses, made from mixed milk from cows, sheepand/or goats - soft and semi-soft - made frompasteurised milk - Processing plant - Surveillance


Officialsampling


Cheeses, made from mixed milk from cows, sheepand/or goats - soft and semi-soft - made from raw orlow heat-treated milk - Processing plant -Surveillance




S. EnteritidisS.

Typhimurium

20


Portugal - 2013




Cheeses, made from mixed milk from cows, sheepand/or goats - soft and semi-soft - made from raw orlow heat-treated milk - Retail - Surveillance



Cheeses, made from mixed milk from cows, sheepand/or goats - unspecified - made from raw or lowheat-treated milk - Retail - Surveillance



Cheeses, made from unspecified milk or otheranimal milk - hard - made from raw or low heat-treated milk - Retail - Surveillance



Cheeses, made from unspecified milk or otheranimal milk - soft and semi-soft - made from raw orlow heat-treated milk - Retail - Surveillance


Officialsampling food sample Domestic Single 25 Gram 1 0Dairy products (excluding cheeses) - dairy desserts -





S. EnteritidisS.

Typhimurium



S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecifiedS. Anatum

21


Portugal - 2013


1Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk - Retail- Surveillance






Cheeses made from goats' milk - hard - made fromraw or low heat-treated milk - Retail - Surveillance


Cheeses made from sheep's milk - fresh -Processing plant - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,


22


Portugal - 2013










S. 1,4,[5],12:i:-

Salmonellaspp.,


23


Portugal - 2013




Dairy products (excluding cheeses) - dairy desserts -Retail - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,


24


Portugal - 2013

Table Salmonella in other food


Officialsampling food sample Domestic Single 25 Gram 3 0Eggs - raw material (liquid egg) for egg products -



Officialsampling food sample Domestic Batch 25 Gram 5 0Molluscan shellfish - cooked - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 170 3Live bivalve molluscs - unspecified - Retail -

Surveillance


Officialsampling food sample Domestic Batch 25 Gram 25 0Fruits - pre-cut - ready-to-eat - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 15 0Vegetables - pre-cut - ready-to-eat - Retail -

Surveillance


Officialsampling food sample Domestic Single 25 Gram 1 0Bakery products - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 65 3Coconut - coconut products - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 75 0Crustaceans - prawns - cooked - chilled - Retail -

Surveillance


Officialsampling food sample Unknown Batch 25 Gram 10 0Crustaceans - unspecified - cooked - Border



Officialsampling food sample Domestic Single 25 Gram 8 0Crustaceans - unspecified - cooked - chilled -



Officialsampling food sample Domestic Single 25 Gram 49 0Eggs - table eggs - Processing plant - Surveillance


Officialsampling food sample Domestic Single 25 Gram 2 0Fishery products, unspecified - ready-to-eat -





S. EnteritidisS.

Typhimurium

25


Portugal - 2013



HACCP andown checks food sample Domestic Single 25 Gram 72 0Fruits - pre-cut - ready-to-eat - Catering -

Surveillance


HACCP andown checks

food sample> milk Domestic Batch 25 Gram 18 0Infant formula - ready-to-eat - Hospital or medical

care facility - Surveillance


Officialsampling food sample Domestic Single 25 Millilitre 4 0Juice - fruit juice - pasteurised - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 10 0Live bivalve molluscs - mussels - Retail -

Surveillance


Officialsampling food sample Domestic Batch 25 Gram 15 0Live bivalve molluscs - oysters - Retail -

Surveillance


Officialsampling food sample Domestic Single 25 Gram 103 0Live bivalve molluscs - unspecified - depurated -



Officialsampling food sample Unknown Batch 25 Gram 165 12Molluscan shellfish - cooked - Border inspection

activities - Surveillance


Officialsampling food sample Domestic Single 10 Gram 1 0Other processed food products and prepared dishes

- Retail - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 25 0Other processed food products and prepared dishes




- noodles - Retail - Surveillance



- pasta - Retail - Surveillance


HACCP andown checks food sample Domestic Single 25 Gram 33 0Other processed food products and prepared dishes

- sandwiches - Catering - Surveillance




S. EnteritidisS.

Typhimurium

26


Portugal - 2013




Other processed food products and prepared dishes- unspecified - non-ready-to-eat foods - Retail -Surveillance






Other processed food products and prepared dishes- unspecified - non-ready-to-eat foods - frozen -Retail - Surveillance


HACCP andown checks food sample Domestic Single 25 Gram 239 0

Other processed food products and prepared dishes- unspecified - ready-to-eat foods - Catering -Surveillance



Other processed food products and prepared dishes- unspecified - ready-to-eat foods - Retail -Surveillance





HACCP andown checks food sample Domestic Single 25 Gram 685 0

Other processed food products and prepared dishes- unspecified - ready-to-eat foods - chilled - Catering- Surveillance


HACCP andown checks food sample Domestic Single 25 Gram 147 0Ready-to-eat salads - Catering - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 35 0Ready-to-eat salads - Retail - Surveillance


Officialsampling food sample Domestic Single 25 Gram 1 0Ready-to-eat salads - Retail - Surveillance


Officialsampling food sample Domestic Single 25 Gram 3 0Sauce and dressings - Retail - Surveillance




S. EnteritidisS.

Typhimurium

27


Portugal - 2013



Officialsampling food sample Domestic Single 25 Gram 7 0Soups - ready-to-eat - Hospital or medical care

facility - Surveillance


Officialsampling food sample Domestic Single 25 Gram 4 0Spices and herbs - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 140 0Spices and herbs - Retail - Surveillance

INSA Suspectsampling

Officialsampling food sample Domestic Single 25 Gram 5 0Vegetables - non-pre-cut - Processing plant -

Surveillance




S. EnteritidisS.

Typhimurium

Eggs - raw material (liquid egg) for egg products -Processing plant - Surveillance

Molluscan shellfish - cooked - Retail - Surveillance

2 1Live bivalve molluscs - unspecified - Retail -Surveillance

Fruits - pre-cut - ready-to-eat - Retail - Surveillance

Vegetables - pre-cut - ready-to-eat - Retail -Surveillance

Bakery products - Retail - Surveillance

3Coconut - coconut products - Retail - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified

S.Braenderup S. Bredeney S. Colorado S. Corvallis S. IV

43:z4,z23:-S.

Meleagridis S. Neukoelln S. Reading S. Wagadugu

28


Portugal - 2013


Crustaceans - prawns - cooked - chilled - Retail -Surveillance

Crustaceans - unspecified - cooked - Borderinspection activities - Surveillance

Crustaceans - unspecified - cooked - chilled -Processing plant - Surveillance

Eggs - table eggs - Processing plant - Surveillance

Fishery products, unspecified - ready-to-eat -Processing plant - Surveillance

Fruits - pre-cut - ready-to-eat - Catering -Surveillance

Infant formula - ready-to-eat - Hospital or medicalcare facility - Surveillance

Juice - fruit juice - pasteurised - Retail - Surveillance

Live bivalve molluscs - mussels - Retail -Surveillance

Live bivalve molluscs - oysters - Retail -Surveillance

Live bivalve molluscs - unspecified - depurated -Processing plant - Surveillance

2 1 1 1 1 1 1Molluscan shellfish - cooked - Border inspectionactivities - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified


43:z4,z23:-S.


29


Portugal - 2013


Other processed food products and prepared dishes- Retail - Surveillance


Other processed food products and prepared dishes- noodles - Retail - Surveillance

Other processed food products and prepared dishes- pasta - Retail - Surveillance

Other processed food products and prepared dishes- sandwiches - Catering - Surveillance





2Other processed food products and prepared dishes- unspecified - ready-to-eat foods - Retail -Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified


43:z4,z23:-S.


30


Portugal - 2013




Ready-to-eat salads - Catering - Surveillance

Ready-to-eat salads - Retail - Surveillance


Sauce and dressings - Retail - Surveillance

Soups - ready-to-eat - Hospital or medical carefacility - Surveillance

Spices and herbs - Retail - Surveillance


Vegetables - non-pre-cut - Processing plant -Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified


43:z4,z23:-S.


Eggs - raw material (liquid egg) for egg products -Processing plant - Surveillance

S.Weltevreden

31


Portugal - 2013


Molluscan shellfish - cooked - Retail - Surveillance

Live bivalve molluscs - unspecified - Retail -Surveillance

Fruits - pre-cut - ready-to-eat - Retail - Surveillance

Vegetables - pre-cut - ready-to-eat - Retail -Surveillance

Bakery products - Retail - Surveillance

Coconut - coconut products - Retail - Surveillance

Crustaceans - prawns - cooked - chilled - Retail -Surveillance

Crustaceans - unspecified - cooked - Borderinspection activities - Surveillance

Crustaceans - unspecified - cooked - chilled -Processing plant - Surveillance

Eggs - table eggs - Processing plant - Surveillance

Fishery products, unspecified - ready-to-eat -Processing plant - Surveillance

Fruits - pre-cut - ready-to-eat - Catering -Surveillance

Infant formula - ready-to-eat - Hospital or medicalcare facility - Surveillance

S.Weltevreden

32


Portugal - 2013


Juice - fruit juice - pasteurised - Retail - Surveillance

Live bivalve molluscs - mussels - Retail -Surveillance

Live bivalve molluscs - oysters - Retail -Surveillance

Live bivalve molluscs - unspecified - depurated -Processing plant - Surveillance

4Molluscan shellfish - cooked - Border inspectionactivities - Surveillance



Other processed food products and prepared dishes- noodles - Retail - Surveillance

Other processed food products and prepared dishes- pasta - Retail - Surveillance

Other processed food products and prepared dishes- sandwiches - Catering - Surveillance



S.Weltevreden

33


Portugal - 2013







Ready-to-eat salads - Catering - Surveillance



Sauce and dressings - Retail - Surveillance

Soups - ready-to-eat - Hospital or medical carefacility - Surveillance




S.Weltevreden

34


Portugal - 2013


35


Portugal - 2013

Table Salmonella in red meat and products thereof


Officialsampling

food sample> meat Domestic Single 25 Gram 52 3Meat from pig - fresh - Processing plant -

Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 38 2Meat from pig - meat preparation - intended to be

eaten cooked - Processing plant - Surveillance


Officialsampling


Meat from pig - meat products - raw but intended tobe eaten cooked - Processing plant - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 5 0Meat from pig - meat products - raw but intended to

be eaten cooked - Retail - Surveillance

LRV Objectivesampling

Officialsampling

food sample> meat Domestic Single 25 Gram 66 9 2Meat from pig - meat products - cooked, ready-to-

eat - Processing plant - Surveillance


Officialsampling

food sample> meat Domestic Batch 400 Square

centimetre 282 7 3Meat from bovine animals - carcase -Slaughterhouse - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 26 0Meat from bovine animals - fresh - Processing plant

- Surveillance


Officialsampling


Meat from bovine animals - meat preparation -intended to be eaten cooked - Processing plant -Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 13 1Meat from sheep - fresh - Processing plant -

Surveillance


Officialsampling food sample Domestic Single 10 Gram 2 0Meat from bovine animals - fresh - chilled - Retail -

Surveillance



Meat from bovine animals - meat preparation -intended to be eaten cooked - chilled - Retail -Surveillance




S. EnteritidisS.

Typhimurium

36


Portugal - 2013




Meat from bovine animals - meat preparation -intended to be eaten cooked - frozen - Retail -Surveillance



Meat from bovine animals - minced meat - intendedto be eaten cooked - chilled - Retail - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 105 10 2

Meat from bovine animals and pig - meatpreparation - intended to be eaten cooked - Retail -Surveillance



Meat from bovine animals and pig - minced meat -intended to be eaten cooked - Retail - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 8 0Meat from goat - fresh - Processing plant -

Surveillance


Officialsampling food sample Domestic Single 10 Gram 1 0Meat from pig - fresh - chilled - Retail - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 95 7Meat from pig - meat preparation - intended to be



Officialsampling food sample Domestic Batch 25 Gram 5 0Meat from pig - meat preparation - intended to be

eaten cooked - frozen - Retail - Surveillance


Officialsampling food sample Domestic Single 25 Gram 1 0Meat from pig - meat products - cooked ham - sliced



Officialsampling

food sample> meat Domestic Single 25 Gram 118 5Meat from pig - meat products - cooked, ready-to-



Officialsampling food sample Domestic Batch 25 Gram 5 0Meat from pig - meat products - cooked, ready-to-

eat - chilled - Retail - Surveillance




S. EnteritidisS.

Typhimurium

37


Portugal - 2013



Officialsampling food sample Domestic Batch 25 Gram 105 0Meat from pig - meat products - fermented sausages



Officialsampling food sample Domestic Single 25 Gram 4 0Meat from pig - meat products - fermented sausages




Meat from pig - meat products - raw but intended tobe eaten cooked - chilled - Retail - Surveillance


Officialsampling food sample Domestic Batch 25 Gram 20 0Meat from pig - meat products - raw ham - Retail -

Surveillance


Officialsampling food sample Domestic Single 10 Gram 1 0Meat from pig - minced meat - intended to be eaten

cooked - chilled - Retail - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 11 1 1

Meat, mixed meat - meat preparation - intended tobe eaten cooked - Processing plant - Surveillance



Meat, mixed meat - meat preparation - intended tobe eaten cooked - chilled - Retail - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 45 0Meat, mixed meat - meat products - Retail -

Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 9 0Meat, mixed meat - meat products - cooked, ready-

to-eat - Processing plant - Surveillance


Officialsampling food sample Domestic Single 10 Gram 10 0Meat, mixed meat - minced meat - intended to be





S. EnteritidisS.

Typhimurium

38


Portugal - 2013


1 1 1Meat from pig - fresh - Processing plant -Surveillance

2Meat from pig - meat preparation - intended to beeaten cooked - Processing plant - Surveillance

1Meat from pig - meat products - raw but intended tobe eaten cooked - Processing plant - Surveillance

Meat from pig - meat products - raw but intended tobe eaten cooked - Retail - Surveillance

7Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Surveillance

4Meat from bovine animals - carcase -Slaughterhouse - Surveillance

Meat from bovine animals - fresh - Processing plant- Surveillance

1Meat from bovine animals - meat preparation -intended to be eaten cooked - Processing plant -Surveillance

1Meat from sheep - fresh - Processing plant -Surveillance

Meat from bovine animals - fresh - chilled - Retail -Surveillance

2Meat from bovine animals - meat preparation -intended to be eaten cooked - chilled - Retail -Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecifiedS. 4,12:i:- S. 4,5,12:i:-

S.Bovismorbific

ansS. Derby S. Infantis S. London S. Reading

39


Portugal - 2013


Meat from bovine animals - meat preparation -intended to be eaten cooked - frozen - Retail -Surveillance

Meat from bovine animals - minced meat - intendedto be eaten cooked - chilled - Retail - Surveillance

2 1 5Meat from bovine animals and pig - meatpreparation - intended to be eaten cooked - Retail -Surveillance

2Meat from bovine animals and pig - minced meat -intended to be eaten cooked - Retail - Surveillance

Meat from goat - fresh - Processing plant -Surveillance

Meat from pig - fresh - chilled - Retail - Surveillance

6 1Meat from pig - meat preparation - intended to beeaten cooked - chilled - Retail - Surveillance

Meat from pig - meat preparation - intended to beeaten cooked - frozen - Retail - Surveillance

Meat from pig - meat products - cooked ham - sliced- Retail - Surveillance

2 1 1 1Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Surveillance

Meat from pig - meat products - cooked, ready-to-eat - chilled - Retail - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,


S.Bovismorbific


40


Portugal - 2013


Meat from pig - meat products - fermented sausages- Retail - Surveillance

Meat from pig - meat products - fermented sausages- Retail - Surveillance


Meat from pig - meat products - raw ham - Retail -Surveillance

Meat from pig - minced meat - intended to be eatencooked - chilled - Retail - Surveillance


2 1Meat, mixed meat - meat preparation - intended tobe eaten cooked - chilled - Retail - Surveillance

Meat, mixed meat - meat products - Retail -Surveillance

Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Surveillance

Meat, mixed meat - minced meat - intended to beeaten cooked - chilled - Retail - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,


S.Bovismorbific



2.1.4 Salmonella in animals

Monitoring systemSampling strategy

Breeding flocks (separate elite, grand parent and parent flocks when necessary)The sampling frame shall cover all adult breeding flocks of Gallus gallus comprising at least 250 birds.

Sampling is accomplished by the operator and by the official authority.

Sampling is done at the holding.

At the iniciative of the operator samples will be taken at day old, 4 weeks old birds, 2 weeks before layingphase and during the laying period, every two weeks.

At day-old sampling shall consist of internal linings of delivery boxes and dead chicks.

At 4 weeks old and at two weeks before the laying phase sampling shall consist of pooled faeces made upof separate samples of fresh faeces each weighing no less than 1 g taken at random from a number ofsites in the building in which the birds are kept.

During the laying phase sampling will consist of 5 boot swabs representative of all parts of the house.

In cage breeding flocks, sampling consists of naturally mixed faeces from dropping belts, scrapers or deeppits 2 samples of at least 150 g will be collected to be tested individually.

At the initiative of the official services sampling is done at :

Within four weeks following moving to laying phase or laying unit (24 weeks);

During the production (44 weeks);

Towards the end of the laying phase not earlier than 8 weeks before the end of the production cycle.Frequency of the sampling

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Day-old chicksEvery flock is sampled.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Rearing periodAt the age of 4 weeks and 2 weeks before moving to the laying phase.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Production periodEvery 2 weeks.

Type of specimen takenBreeding flocks (separate elite, grand parent and parent flocks when necessary): Day-old chicks

A. Salmonella spp. in Gallus Gallus - breeding flocks

41Portugal - 2013


Other: Internal linings of delivery boxes and dead chicks.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Rearing periodFaeces.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Production periodFaeces (boot swabs).

Methods of sampling (description of sampling techniques)Breeding flocks (separate elite, grand parent and parent flocks when necessary): Day-old chicks

The sample shall consist of a minimum of one composite sample of visibly soiled hatcher basket liners.

The FBO must sample all dead birds at arrival.Breeding flocks (separate elite, grand parent and parent flocks when necessary): Rearing period

At 4 weeks old and 2 weeks before the laying phase the sampling will consist of faecal samples.

Pooled faeces made up of separate samples of fresh faeces each weighing no less than 1 g taken atrandom from a number of sites in the building in which the birds are kept.

Breeding flocks: Production periodDuring the laying phase 5 Pairs of boot swabs – walking around to be done in a way which will samplerepresentatively all parts of the sector. .

In cage breeding flocks, sampling consists of naturally mixed faeces from dropping belts, scrapers or deeppits. 2 samples of at least 150 g will be collected to be tested individually.

Case definitionBreeding flocks (separate elite, grand parent and parent flocks when necessary): Day-old chicks

At least one positive sample to S. Enteritidis, S. Typhimurium, S. Typhimurium- Like, S. Hadar, S. Virchowand / or S. Infantis.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Rearing periodAt least one positive sample to S. Enteritidis, S. Typhimurium, S. Typhimurium- Like, S. Hadar, S. Virchowand / or S. Infantis.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Production periodAt least one positive sample to S. Enteritidis, S. Typhimurium, S. Typhimurium- Like, S. Hadar, S. Virchowand / or S. Infantis.

Diagnostic/analytical methods usedBreeding flocks (separate elite, grand parent and parent flocks when necessary): Day-old chicks

Bacteriological method: ISO 6579:2002.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Rearing periodBacteriological method: ISO 6579:2002.

Breeding flocks (separate elite, grand parent and parent flocks when necessary): Production periodBacteriological method: ISO 6579:2002.

Vaccination policyBreeding flocks (separate elite, grand parent and parent flocks when necessary)

42Portugal - 2013


Vaccination is voluntary.Compulsive vaccination against Salmonella Enteritidis is done in the restocking, after the slaughter of apositive flock.

Control program/mechanismsThe control program/strategies in place

Breeding flocks (separate elite, grand parent and parent flocks when necessary)The strategy is to reinforce surveillance, reinforce biosecurity measures, slaughter the positive flocks andrestocking only when environmental samples are negative for Salmonella, with birds from flocks or herdsthat have undergone controls according to the legislation requirements, with negative results. All therestocking birds must be vaccinated against Salmonella Enteritidis.

The strategy includes also a close cooperation with the associations of producers to implement differentmeans to raise awareness of the producers.

The Official Services have developed guidelines for the producer, as a tool in order to guide theimplementation of the national programme.

Measures in case of the positive findings or single casesBreeding flocks (separate elite, grand parent and parent flocks when necessary)

When there is a positive case in a flock = Salmonella sp detection

Notification of the operator;Keep the flock in sanitary surveillance;Forcing to keep the update records;Evaluate the production records;Forcing to incubate the eggs from the suspect flock, separately.

Whenever the results from serotyping are diferent from the serotypes relevant to the national programme ,than:Additional biosecurity measures;Free practice – The official control measures are withdrawn.

When the result is serotype S. Enteritidis, S. Typhimurium, than the flock will be under official restriction:Flock surveillance (under official control);Slaughter of the positive flock;Destruction of the incubated eggs;Non incubated eggs must be destroyed or be treated.

After the destruction of the positive flock the holding and the environment must be cleaned anddisinfected;

The operator must collect environmental samples;

The restocking of animals must take place from flocks or herds that have undergone controls (withnegative results) according to the legislation requirements;

All the restocking birds must be vaccinated against Salmonella Enteritidis.

43Portugal - 2013


44Portugal - 2013



Broiler flocksSampling is accomplished by the food business operator and by the competentauthority.The sampling is done at the holding.Sampling on the initiative of the food business operator shall takeplace within three weeks before the birds are moved to theslaughterhouse.Sampling by the competent authority includes each year at least one flock of broilers on 10 % of theholdings with more than 5 000 birds. It's done on a risk basis and each time the competent authorityconsiders it necessary.

Frequency of the samplingBroiler flocks: Before slaughter at farm

3 weeks prior to slaughter.

Type of specimen takenBroiler flocks: Before slaughter at farm

Faeces (boot swabs)

Methods of sampling (description of sampling techniques)Broiler flocks: Before slaughter at farm

At least two pairs of boot swabs shall be taken. For free range flocksof broilers, samples shall only be collected in the area inside the house.All boot swabs will be pooled into one sample. In flocks with less than 100 broilers, when the access to thehouses is not possible, the boot swabs may be replaced by hand drag swabs and rubbed over surfacescontaminated with fresh faeces, or if not feasible, by other sampling techniques for faeces fit for theintended purpose.

It shall be ensured that all sections in a house are represented in thesampling in a proportionate way. Each pair should cover about 50 % of the area of the house.

On completion of sampling the boot swabs shall be carefully removed so as not to dislodge adherentmaterial. Boot swabs may be inverted to retain material. They shall be placed in a bag or pot and labelled.

Case definitionBroiler flocks: Before slaughter at farm

A flock of broilers is considered positive where the presence of Salmonella Enteritidis (other than vaccinestrains) and/or Salmonella Typhimurium or Salmonella typhimurium -Like is detected in the flock at anyoccasion.

Diagnostic/analytical methods usedBroiler flocks: Before slaughter at farm



B. Salmonella spp. in Gallus Gallus - broiler flocks

45Portugal - 2013


Broiler flocksThe strategy is to reinforce surveillance, reinforce biosecurity measures, slaughter the positive flocks andrestocking only when environmental samples are negative for Salmonella, with birds from flocks or herdsthat have undergone controls according to the legislation requirements.The strategy includes also a closecooperation with the associations of producers to implement different means to raise awareness of theproducers.The Official Services have developed guidelines for the producer, as a tool in order to guide theimplementation of the national programme.

Measures in case of the positive findings or single casesBroiler flocks: Before slaughter at farm

When there is a positive case in a flock = Salmonella sp detectionNotification of the operator;Flock under official control (restriction);Forcing to keep update records.

Whenever the results from serotyping are diferent from the serotypes relevant to the national programme,than:Additional biosecurity measures;Free practice – The official control measures are withdrawn.

When the result is serotype S. Enteritidis and/or S. Typhimurium than the flock will continue under officialrestriction:Flock surveillance (under official control)After the slaugther of the positive flock the holding and the environment must be cleaned and disinfected;The operator must collect environmental samples;The restocking of animals must take place from flocks or herds that haveundergone controls (with negative results) according to the legislation requirements;

46Portugal - 2013



Laying hens flocksThe sampling frame shall cover all flocks of laying hens of Gallus gallus

Sampling is accomplished by the food business operator and by the competent authority.

The sampling is done at the holding.

At the initiative of the operator samples will be taken at day old, 2 weeks before moving to laying phaseand during the laying period, every fifteen weeks. The first sampling at the laying period will take place atthe age of 24 ± 2 weeks.

At the initiative of the official services sampling is done:In one flock per year per holding comprising at least 1 000 birds;At the age of 24 ± 2 weeks in laying flocks housed in buildings where Salmonella was detected in thepreceding flock;In any case of suspicion of Salmonella Enteritidis or Salmonella Typhimurium infection, as a result of theepidemiological investigation of food-borne outbreaks in accordance with Article 8 of Directive 2003/99/ECof the European Parliament and of the CouncilIn all other laying flocks on the holding in case Salmonella Enteritidis or Salmonella Typhimurium aredetected in one laying flock on the holding;In cases where the competent authority considers it appropriate

Sampling protocolIn cage flocks, 2 × 150 grams of naturally pooled faeces taken from all belts or scrapers in the house afterrunning the manure removal system.In step cage houses without scrapers or belts: 2 × 150 grams of mixed fresh faeces collected from 60different places beneath the cages in the dropping pits.In barn or free-range houses, two pairs of boot swabs or socks, without changing overboots between bootswabs.

In the case of sampling by the competent authority, it will be collected an additional sample of 150 gramsnaturally pooled faeces or an additional pair of boot swabs or socks.

Frequency of the samplingLaying hens: Day-old chicks

Other: Internal linings of delivery boxes and dead chicks.

Laying hens: Rearing periodAt the age of ± 18 weeks.

Laying hens: Production periodEvery 15 weeks.

Type of specimen takenLaying hens: Day-old chicks

C. Salmonella spp. in Gallus Gallus - flocks of laying hens

47Portugal - 2013


Internal linings of delivery boxes.

Laying hens: Rearing periodFaeces.

Laying hens: Production periodEnvironmental sample: faeces.

Methods of sampling (description of sampling techniques)Laying hens: Day-old chicks

The sample shall consist of a minimum of one composite sample of visibly soiled hatcher basket liners.FBO must also sample all dead birds at arrival.

Laying hens: Rearing period* In cage flocks, 2 × 150 grams of naturally pooled faeces taken from all belts or scrapers in the houseafter running the manure removal system.

* In step cage houses without scrapers or belts: 2 × 150 grams of mixed fresh faeces collected from 60different places beneath the cages in the dropping pits.

* In barn or free-range houses, two pairs of boot swabs or socks, without changing overboots betweenboot swabs.

Laying hens: Production period* In cage flocks, 2 × 150 grams of naturally pooled faeces taken from all belts or scrapers in the houseafter running the manure removal system.

* In step cage houses without scrapers or belts: 2 × 150 grams of mixed fresh faeces collected from 60different places beneath the cages in the dropping pits.

* In barn or free-range houses, two pairs of boot swabs or socks, without changing overboots betweenboot swabs.

In the case of sampling by the competent authority, it will be collected an additional sample of 150 gramsnaturally pooled faeces or an additional pair of boot swabs or socks.

Case definitionLaying hens: Day-old chicks

At least one positive sample to S. Enteritidis and/or S. Typhimurium (including S.T-Like).

Laying hens: Rearing periodAt least one positive sample to S. Enteritidis and/or S. Typhimurium (including S.T-Like).

Laying hens: Production periodAt least one positive sample to S. Enteritidis and/or S. Typhimurium(including S.T-Like).

Diagnostic/analytical methods usedLaying hens: Day-old chicks


Laying hens: Rearing period

48Portugal - 2013



Laying hens: Production periodBacteriological method: ISO 6579:2002.

Vaccination policyLaying hens flocks

Vaccination programmes against Salmonella Enteritidis are applied during the rearing phase.


Laying hens flocksThe strategy is to reinforce surveillance, reinforce biosecurity measures, slaughter the positive flocks andrestocking only when environmental samples are negative for Salmonella, with birds from flocks or herdsthat have undergone controls according to the legislation requirements.

The eggs of the positive flock will be destructed or send to heat treated egg-products .

All birds must be vaccinated against Salmonella Enteritidis.

The strategy includes also a close cooperation with the associations of producers to implement differentmeans to raise awareness of the producers.


Measures in case of the positive findings or single casesLaying hens flocks

When there is a positive case in a flock = Salmonella sp detection

Notification of the operator;Keep the flock in sanitary surveillance;Forcing to keep the update records;Evaluate the production records.Keep the eggs in the holding or send them to eggs products.

Whenever the results from serotyping are diferent from the serotypes relevant to the national programme ,than:Additional biosecurity measuresFree practice – The official control measures are withdrawn.

When the result is serotype S. Enteritidis and/or S. Typhimurium than the flock will be under officialrestriction:

Flock surveillance (under official control)Eggs must be destroyed or be treatedAfter the destruction of the positive flock the holding and the environment must be cleaned anddisinfected;The operator must collect environmental samples;The restocking of animals must take place from flocks or herds that have undergone controls according to

49Portugal - 2013


the legislation requirements;

All birds must be vaccinated against Salmonella Enteritidis.

50Portugal - 2013



Breeding flocks (separate elite, grand parent and parent flocks when necessary)There are no breeding flocks of turkeys in Portugal.

Meat production flocksSampling is accomplished by the food business operator and by the competent authority.The sampling is done at the holding.Sampling on the initiative of the food business operator takes place within three weeks before the birdsare moved to the slaughterhouse.

Sampling by the competent authority includes once a year, all flocks on 10 % of the holdings with at least500 fattening turkeys and:— all flocks on the holding when one flock tested positive for Salmonella Enteritidis orSalmonellaTyphimurium in samples taken by the food business operator, unless the meat of the turkeysin the flocks is destined for industrial heat treatment or another treatment to eliminate salmonella, and— all flocks on the holding when one flock tested positive for Salmonella enteritidis or Salmonellatyphimurium during the previous round in samples taken by the food business operator, and— each time the competent authority considers it necessary.

Frequency of the samplingMeat production flocks: Before slaughter at farm

3 weeks prior to slaughter

Type of specimen takenMeat production flocks: Before slaughter at farm

Faeces

Methods of sampling (description of sampling techniques)Meat production flocks: Before slaughter at farm

At least two pairs of boot swabs shall be taken. For free range flocks, samples will only be collected in thearea inside the house.All boot swabs must be pooled into one sample.In flocks with less than 100 turkeys, where it is not possible to useboot swabs as access to the houses is not possible, they may bereplaced by hand drag swabs, where the boot swabs or socks are wornover gloved hands and rubbed over surfaces contaminated with freshfaeces, or if not feasible, by other sampling techniques for faeces fitfor the intended purpose.It shall be ensured that all sections in a house are represented in the sampling ina proportionate way. Each pair should cover about 50 % of the area of the house.

On completion of sampling the boot/sock swabs shall be carefullyremoved so as not to dislodge adherent material. Boot swabs may beinverted to retain material. They shall be placed in a bag or pot andlabelled.

Case definitionA flock of turkeys is considered positive where the presence of Salmonella Enteritidis and/or Salmonella

D. Salmonella spp. in turkey - breeding flocks and meat production flocks

51Portugal - 2013


Typhimurium including Salmonella Typhimurium -Like (other than vaccine strains) is detected in the flockat any occasion.

Monitoring systemCase definition

Meat production flocks: Before slaughter at farmA flock of turkeys is considered positive where the presence of Salmonella enteritidis and/or Salmonellatyphimurium including Salmonella Typhimurium -Like (other than vaccine strains) isdetected in the flock at any occasion.

Diagnostic/analytical methods usedMeat production flocks: Before slaughter at farm



Meat production flocksThe strategy is to reinforce surveillance, reinforce biosecurity measures, slaughter the positive flocks andrestocking only when environmental samples are negative for Salmonella, with birds from flocks or herdsthat have undergone controls (with negative results) according to the legislation requirements.The strategyincludes also a close cooperation with the associations of producers to implement different means to raiseawareness of the producers.


Measures in case of the positive findings or single casesWhen there is a positive case in a flock = Salmonella sp detectionNotification of the operator;Flock surveillance (under official control);Forcing to keep update records;Evaluate the production records;

Whenever the results from serotyping are diferent from the serotypes relevant to the national programme,than:Additional biosecurity measuresFree practice – The official control measures are withdrawn.

When the result is serotype S. Enteritidis and/or S. Typhimurium than the flock will be under officialrestriction:Flock surveillance (under official control)

After the slaugther of the positive flock the holding and the environment must be cleaned and disinfected;The operator must collect environmental samples;The restocking of animals must take place from flocks or herds that have undergone controls according tothe legislation requirements;

52Portugal - 2013

53


Portugal - 2013

Table Salmonella in breeding flocks of Gallus gallus

16 Suspectsampling

Official andindustrysampling

environmental sample >boot swabs

Domestic no herd/flock 16 16 2Gallus gallus (fowl) - breeding flocks for broilerproduction line - adult - Farm - Control anderadication programmes

1)

488 CensusOfficial and

industrysampling


Domestic yes herd/flock 488 9Gallus gallus (fowl) - breeding flocks for broilerproduction line - adult - Farm - Control anderadication programmes

2)


industrysampling


Domestic yes herd/flock 20 0Gallus gallus (fowl) - breeding flocks for eggproduction line - adult - Farm - Control anderadication programmes

No of flocksunder controlprogramme


Samplingstrategy Sampler Sample type Sample origin Target

VerificationSampling unit Units tested

Total unitspositive forSalmonella

S. Enteritidis

1 4 4 3 1Gallus gallus (fowl) - breeding flocks for broilerproduction line - adult - Farm - Control anderadication programmes

1)

1 2 2 1 1 1Gallus gallus (fowl) - breeding flocks for broilerproduction line - adult - Farm - Control anderadication programmes

2)

Gallus gallus (fowl) - breeding flocks for eggproduction line - adult - Farm - Control anderadication programmes

S. Hadar S. InfantisS.

Typhimurium S. Virchow S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified

Otherserovars S. Bredeney S.

DuesseldorfS. II

42:b:e,n,x,z15

S. Mbandaka

54


Portugal - 2013

Table Salmonella in breeding flocks of Gallus gallus

Comments:1) Number of positive samples - Number of isolates in the lab2) Salmonella - S. I 7:-:-

1Gallus gallus (fowl) - breeding flocks for broilerproduction line - adult - Farm - Control anderadication programmes

1)

1Gallus gallus (fowl) - breeding flocks for broilerproduction line - adult - Farm - Control anderadication programmes

2)

Gallus gallus (fowl) - breeding flocks for eggproduction line - adult - Farm - Control anderadication programmes

S.Senftenberg

55


Portugal - 2013

Table Salmonella in other poultry

41 Suspectsampling


animalsample >

faecesDomestic no herd/flock 41 41 11Gallus gallus (fowl) - laying hens - adult - Farm -

Control and eradication programmes

1)

11149 Census Officialsampling


Domestic yes herd/flock 147 4Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

11149 Census Industrysampling


Domestic yes herd/flock 10983 38 6Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

2)

42 Suspectsampling



Domestic no herd/flock 42 42 6Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

3)

813 Census Officialsampling


Domestic yes herd/flock 57 0Turkeys - fattening flocks - before slaughter - Farm -Control and eradication programmes

813 Census Industrysampling




industrysampling




industrysampling


Domestic yes herd/flock 11130 42 6Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

4)


industrysampling

animalsample >

faecesDomestic yes herd/flock 383 24 6Gallus gallus (fowl) - laying hens - adult - Farm -

Control and eradication programmes

5)

8 Suspectsampling

Industrysampling


Domestic no herd/flock 8 8Turkeys - unspecified - before slaughter - Farm -Control and eradication programmes

6)

No of flocksunder controlprogramme


Samplingstrategy Sampler Sample type Sample origin Target

VerificationSampling unit Units tested

Total unitspositive forSalmonella

S. Enteritidis

56


Portugal - 2013


2 1 3 1 3 2 1 1Gallus gallus (fowl) - laying hens - adult - Farm -Control and eradication programmes

1)

2 1Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

3 1 1 22Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

2)

3 2 1 1 23Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

3)

Turkeys - fattening flocks - before slaughter - Farm -Control and eradication programmes

2 2 1 3Turkeys - fattening flocks - before slaughter - Farm -Control and eradication programmes

2 2 1 3Turkeys - fattening flocks - before slaughter - Farm -Control and eradication programmes


4)

3 1 2 2 1 1Gallus gallus (fowl) - laying hens - adult - Farm -Control and eradication programmes

5)

2 2 1 3Turkeys - unspecified - before slaughter - Farm -Control and eradication programmes

6)

S.Typhimurium S. 1,4,[5],12:i:

-

Salmonellaspp.,

unspecified

Otherserovars S. 4,5,12:i:- S. Agona S. Anatum S. Cerro S. Cubana S. Derby S. Havana

57


Portugal - 2013


Comments:1) Number of positive samples - Number of isolates in the lab2) Salmonella - S. II 48:z10:[1,5]


1)

1Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

1 2 1 1Gallus gallus (fowl) - broilers - before slaughter -Farm - Control and eradication programmes

2)


3)





4)


5)

Turkeys - unspecified - before slaughter - Farm -Control and eradication programmes

6)

S. Landau S. Lexington S.Livingstone S. Mbandaka S. Muenchen S. Redba S. Rissen S.

Senftenberg S. Tomegbe S. Virchow

58


Portugal - 2013


Comments:3) Number of positive samples - Number of isolates in the lab§Number of positive samples - Number of isolates in the lab- S.II 48:z10:[1,5]4) Salmonella - S. II 48:z10:[1,5]5) Salmonella - S. I 9,46:-:- (1)

Salmonella - S. 1,3,19:-:-:z27 (1)Salmonella - S. II 4,[5],12:b (1)

6) Number of positive samples - Number of isolates in the lab

59


Portugal - 2013

2.1.5 Salmonella in feedingstuffs

Table Salmonella in compound feedingstuffs


Officialsampling feed sample Domestic Batch 25 Gram 5 0Compound feedingstuffs for cattle - final product -

Feed mill - Surveillance


Officialsampling feed sample Domestic Single 25 Gram 44 1Compound feedingstuffs for pigs - final product -



Officialsampling feed sample Domestic Single 25 Gram 11 0Compound feedingstuffs for poultry - laying hens -

final product - Feed mill - Surveillance


Officialsampling feed sample Domestic Single 25 Gram 20 0Compound feedingstuffs for poultry - broilers - final

product - Feed mill - Surveillance


Officialsampling feed sample Domestic Single 25 Gram 45 0Compound feedingstuffs for cattle - final product -



Officialsampling feed sample Domestic Single 25 Gram 3 0Compound feedingstuffs for fish - final product -



Officialsampling feed sample Domestic Single 26 Gram 5 0Compound feedingstuffs for horses - final product -



Officialsampling feed sample Domestic Single 25 Gram 2 0

Compound feedingstuffs for poultry (non specified) -final product - non-pelleted/meal - Feed mill -Surveillance


Officialsampling feed sample Domestic Single 25 Gram 5 0Compound feedingstuffs for rabbits - final product -



Officialsampling feed sample Domestic Single 25 Gram 10 0Compound feedingstuffs for sheep - final product -





S. EnteritidisS.

Typhimurium

60


Portugal - 2013

Table Salmonella in compound feedingstuffs

Compound feedingstuffs for cattle - final product -Feed mill - Surveillance

1Compound feedingstuffs for pigs - final product -Feed mill - Surveillance

Compound feedingstuffs for poultry - laying hens -final product - Feed mill - Surveillance

Compound feedingstuffs for poultry - broilers - finalproduct - Feed mill - Surveillance

Compound feedingstuffs for cattle - final product -Feed mill - Surveillance

Compound feedingstuffs for fish - final product -Feed mill - Surveillance

Compound feedingstuffs for horses - final product -Feed mill - Surveillance

Compound feedingstuffs for poultry (non specified) -final product - non-pelleted/meal - Feed mill -Surveillance

Compound feedingstuffs for rabbits - final product -Feed mill - Surveillance

Compound feedingstuffs for sheep - final product -Feed mill - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified

61


Portugal - 2013

Table Salmonella in other feed matter


Officialsampling feed sample Domestic Batch 25 Gram 10 0Feed material of cereal grain origin - wheat derived -



Officialsampling feed sample Domestic Batch 25 Gram 10 0Feed material of oil seed or fruit origin - soya (bean)

derived - Feed mill - Surveillance


Officialsampling feed sample Domestic Single 25 Gram 24 0Feed material of cereal grain origin - Feed mill -

Surveillance


Officialsampling feed sample Domestic Batch 25 Gram 5 0Feed material of oil seed or fruit origin - other - Feed

mill - Surveillance


Officialsampling feed sample Domestic Single 25 Gram 1 0Pet food - final product - Feed mill - Surveillance




S. EnteritidisS.

Typhimurium

Feed material of cereal grain origin - wheat derived -Feed mill - Surveillance

Feed material of oil seed or fruit origin - soya (bean)derived - Feed mill - Surveillance

Feed material of cereal grain origin - Feed mill -Surveillance

Feed material of oil seed or fruit origin - other - Feedmill - Surveillance

Pet food - final product - Feed mill - Surveillance

S. 1,4,[5],12:i:-

Salmonellaspp.,

unspecified

62


Portugal - 2013

Table Salmonella in other feed matter

63


Portugal - 2013

2.1.6 Antimicrobial resistance in Salmonella isolates

Table Antimicrobial susceptibility testing of Salmonella in humans

42 0 35 1 154 10Aminoglycosides - Gentamicin

42 0 35 0 154 4Aminoglycosides - Kanamycin

42 2 35 26 154 131Aminoglycosides - Streptomycin

42 0 35 18 154 17Amphenicols - Chloramphenicol

42 0 35 0 154 2Cephalosporins - 3rd generation cephalosporins

42 0 35 1 154 0Fluoroquinolones - Ciprofloxacin

42 18 35 28 154 130Penicillins - Ampicillin

42 22 35 1 154 6Quinolones - Nalidixic acid

42 1 35 28 154 129Tetracyclines - Tetracycline

42 4 35 5 154 6Fully sensitive

42 34 35 2 154 9Resistant to 1 antimicrobial

42 2 35 5 154 20Resistant to 2 antimicrobials



42 1 35 2 154 13Resistant to >4 antimicrobials

42 1 35 1 154 1Number of multiresistant S. Typhimurium - withpenta resistance

42 2 35 2 154 20Number of multiresistant S. Typhimurium -resistant to other antimicrobials

S. Enteritidis S. TyphimuriumSalmonella spp.

no no no

42 35 154

Antimicrobials:

Isolates out of a monitoringprogram (yes/no)

Number of isolates availablein the laboratory

Salmonella

N n N n N n

64


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella in humans

65


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in All feedingstuffs - Surveillance - Objective sampling - Official sampling - feedsample - quantitative data [Dilution method]

2 10 0 4 6Aminoglycosides - Gentamicin

16 10 0 5 5Aminoglycosides - Streptomycin

16 10 0 5 5Amphenicols - Chloramphenicol

0.5 10 0 4 6Cephalosporins - Cefotaxime

0.064 10 0 3 7Fluoroquinolones - Ciprofloxacin


16 10 0 3 7Quinolones - Nalidixic acid

256 10 0 1 1 2 6Sulfonamides

8 10 0 2 8Tetracyclines - Tetracycline

2 10 0 9 1Trimethoprim

All feedingstuffs - Surveillance

no

10

Antimicrobials:



Salmonella spp.

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096

0.25 32Aminoglycosides - Gentamicin

2 512Aminoglycosides - Streptomycin

2 256Amphenicols - Chloramphenicol

All feedingstuffs- Surveillance

no

10

Antimicrobials:



Salmonella spp.

lowest highest

Concentration (µg/ml), number of isolates with a concentration of inhibition equal to

66


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in All feedingstuffs - Surveillance - Objective sampling - Official sampling - feedsample - quantitative data [Dilution method]

0.06 8Cephalosporins - Cefotaxime

0.008 8Fluoroquinolones - Ciprofloxacin

0.5 64Penicillins - Ampicillin

2 512Quinolones - Nalidixic acid

8 1024Sulfonamides

0.5 64Tetracyclines - Tetracycline

0.25 32Trimethoprim


no

10

Antimicrobials:



Salmonella spp.

lowest highest

67


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Enteritidis in All feedingstuffs - Surveillance - Objective sampling - Official sampling - feed sample- quantitative data [Dilution method]


16 5 0 5Aminoglycosides - Streptomycin

16 5 0 5Amphenicols - Chloramphenicol


0.064 5 0 5Fluoroquinolones - Ciprofloxacin

8 5 0 4 1Penicillins - Ampicillin

16 5 0 5Quinolones - Nalidixic acid

256 5 0 1 4Sulfonamides


2 5 0 5Trimethoprim

All feedingstuffs - Surveillance

no

5

Antimicrobials:



S. Enteritidis

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096





no

5

Antimicrobials:



S. Enteritidis

lowest highest


68


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Enteritidis in All feedingstuffs - Surveillance - Objective sampling - Official sampling - feed sample- quantitative data [Dilution method]





8 1024Sulfonamides


0.25 32Trimethoprim


no

5

Antimicrobials:



S. Enteritidis

lowest highest

69


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Meat from poultry, unspecified - fresh - chilled - Surveillance - Objective sampling- Official sampling - food sample - meat - quantitative data [Dilution method]









8 15 0 15Tetracyclines - Tetracycline

2 15 0 15Trimethoprim

Meat from poultry, unspecified - fresh - chilled - Surveillance

no

15

Antimicrobials:



S. Typhimurium

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Meat frompoultry,

unspecified -fresh - chilled -

Surveillance

no

15

Antimicrobials:



S. Typhimurium

lowest highest


70


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Meat from poultry, unspecified - fresh - chilled - Surveillance - Objective sampling- Official sampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim

Meat frompoultry,


Surveillance

no

15

Antimicrobials:



S. Typhimurium

lowest highest

71


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from pig - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]



16 6 1 1 4 1Amphenicols - Chloramphenicol







2 5 0 5Trimethoprim

Meat from pig - fresh - chilled - Surveillance

no

5

Antimicrobials:



S. 4,5,12:i:-

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




Meat from pig -fresh - chilled -

Surveillance

no

5

Antimicrobials:



S. 4,5,12:i:-

lowest highest


72


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from pig - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]





8 1024Sulfonamides


0.25 32Trimethoprim


Surveillance

no

5

Antimicrobials:



S. 4,5,12:i:-

lowest highest

73


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from pig - meat products - cooked, ready-to-eat - Surveillance - Objectivesampling - Official sampling - food sample - meat - quantitative data [Dilution method]

2 1 0 1Aminoglycosides - Gentamicin



0.5 1 0 1Cephalosporins - Cefotaxime


8 1 1 1Penicillins - Ampicillin


256 1 1 1Sulfonamides


2 1 0 1Trimethoprim

Meat from pig - meat products - cooked, ready-to-eat - Surveillance

no

1

Antimicrobials:



S. 4,5,12:i:-

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Meat from pig -meat products -cooked, ready-

to-eat -Surveillance

no

1

Antimicrobials:



S. 4,5,12:i:-

lowest highest


74


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from pig - meat products - cooked, ready-to-eat - Surveillance - Objectivesampling - Official sampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides

Tetracyclines - Tetracycline

Trimethoprim

Meat from pig -meat products -cooked, ready-

to-eat -Surveillance

no

1

Antimicrobials:



S. 4,5,12:i:-

lowest highest

75


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from broilers (Gallus gallus) - meat products - raw but intended to be eatencooked - Surveillance - Objective sampling - Official sampling - food sample - meat - quantitative data [Dilution method]








256 7 1 4 2 1Sulfonamides


2 7 0 7Trimethoprim

Meat from broilers (Gallus gallus) - meat products - raw but intended to be eaten cooked - Surveillance

no

7

Antimicrobials:



S. 4,5,12:i:-

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096


Meat frombroilers (Gallusgallus) - meatproducts - rawbut intended to

be eatencooked -

Surveillance

no

7

Antimicrobials:



S. 4,5,12:i:-

lowest highest


76


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from broilers (Gallus gallus) - meat products - raw but intended to be eatencooked - Surveillance - Objective sampling - Official sampling - food sample - meat - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim

Meat frombroilers (Gallusgallus) - meatproducts - rawbut intended to

be eatencooked -

Surveillance

no

7

Antimicrobials:



S. 4,5,12:i:-

lowest highest

77


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from poultry, unspecified - fresh - chilled - Surveillance - Objective sampling -Official sampling - food sample - meat - quantitative data [Dilution method]










2 2 0 2Trimethoprim


no

2

Antimicrobials:



S. 4,5,12:i:-

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Meat frompoultry,


Surveillance

no

2

Antimicrobials:



S. 4,5,12:i:-

lowest highest


78


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from poultry, unspecified - fresh - chilled - Surveillance - Objective sampling -Official sampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim

Meat frompoultry,


Surveillance

no

2

Antimicrobials:



S. 4,5,12:i:-

lowest highest

79


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from bovine animals - fresh - chilled - Surveillance - Objective sampling - Officialsampling - food sample - meat - quantitative data [Dilution method]










2 1 0 1Trimethoprim

Meat from bovine animals - fresh - chilled - Surveillance

no

1

Antimicrobials:



S. 4,5,12:i:-

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Meat frombovine animals- fresh - chilled- Surveillance

no

1

Antimicrobials:



S. 4,5,12:i:-

lowest highest


80


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Meat from bovine animals - fresh - chilled - Surveillance - Objective sampling - Officialsampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim


no

1

Antimicrobials:



S. 4,5,12:i:-

lowest highest

81


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Meat from poultry, unspecified - fresh - chilled - Surveillance - Objective sampling- Official sampling - food sample - meat - quantitative data [Dilution method]




0.5 6 1 1 3 1 1Cephalosporins - Cefotaxime





8 6 1 1 3 1 1Tetracyclines - Tetracycline

2 6 2 3 1 2Trimethoprim


no

6

Antimicrobials:



Salmonella spp.

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Meat frompoultry,


Surveillance

no

6

Antimicrobials:



Salmonella spp.

lowest highest


82


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Meat from poultry, unspecified - fresh - chilled - Surveillance - Objective sampling- Official sampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim

Meat frompoultry,


Surveillance

no

6

Antimicrobials:



Salmonella spp.

lowest highest

83


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Derby in Meat from pig - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]










2 2 0 2Trimethoprim


no

2

Antimicrobials:



S. Derby

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096





Surveillance

no

2

Antimicrobials:



S. Derby

lowest highest


84


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Derby in Meat from pig - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]





8 1024Sulfonamides


0.25 32Trimethoprim


Surveillance

no

2

Antimicrobials:



S. Derby

lowest highest

85


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Meat from pig - fresh - chilled - Surveillance - Objective sampling - Officialsampling - food sample - meat - quantitative data [Dilution method]












no

3

Antimicrobials:



S. Typhimurium

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096





Surveillance

no

3

Antimicrobials:



S. Typhimurium

lowest highest


86


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Meat from pig - fresh - chilled - Surveillance - Objective sampling - Officialsampling - food sample - meat - quantitative data [Dilution method]





8 1024Sulfonamides


0.25 32Trimethoprim


Surveillance

no

3

Antimicrobials:



S. Typhimurium

lowest highest

87


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Meat from bovine animals - fresh - chilled - Surveillance - Objective sampling -Official sampling - food sample - meat - quantitative data [Dilution method]










2 3 0 3Trimethoprim


no

3

Antimicrobials:



S. Typhimurium

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




no

3

Antimicrobials:



S. Typhimurium

lowest highest


88


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Meat from bovine animals - fresh - chilled - Surveillance - Objective sampling -Official sampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim


no

3

Antimicrobials:



S. Typhimurium

lowest highest

89


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Derby in Meat from bovine animals - fresh - chilled - Surveillance - Objective sampling - Officialsampling - food sample - meat - quantitative data [Dilution method]










2 7 0 7Trimethoprim


no

7

Antimicrobials:



S. Derby

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




no

7

Antimicrobials:



S. Derby

lowest highest


90


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Derby in Meat from bovine animals - fresh - chilled - Surveillance - Objective sampling - Officialsampling - food sample - meat - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim


no

7

Antimicrobials:



S. Derby

lowest highest

91


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Turkeys - fattening flocks - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]










2 2 0 2Trimethoprim

Turkeys - fattening flocks - before slaughter - Control and eradication programmes

no

2

Antimicrobials:



S. Typhimurium

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Turkeys -fattening flocks

- beforeslaughter -Control anderadication

programmes

no

2

Antimicrobials:



S. Typhimurium

lowest highest


92


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Turkeys - fattening flocks - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

2

Antimicrobials:



S. Typhimurium

lowest highest

93


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Turkeys - fattening flocks - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]


16 6 3 2 1 2 1Aminoglycosides - Streptomycin



0.064 6 1 2 2 1 1Fluoroquinolones - Ciprofloxacin





2 5 0 5Trimethoprim

Turkeys - fattening flocks - before slaughter - Control and eradication programmes

no

6

Antimicrobials:



Salmonella spp.

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096





programmes

no

6

Antimicrobials:



Salmonella spp.

lowest highest


94


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Turkeys - fattening flocks - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

6

Antimicrobials:



Salmonella spp.

lowest highest

95


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Gallus gallus (fowl) - broilers - before slaughter - Control and eradication programmes- Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]










2 1 0 1Trimethoprim

Gallus gallus (fowl) - broilers - before slaughter - Control and eradication programmes

no

1

Antimicrobials:



S. 4,5,12:i:-

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096


Gallus gallus(fowl) - broilers


programmes

no

1

Antimicrobials:



S. 4,5,12:i:-

lowest highest


96


Portugal - 2013

Table Antimicrobial susceptibility testing of S. 4,5,12:i:- in Gallus gallus (fowl) - broilers - before slaughter - Control and eradication programmes- Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

1

Antimicrobials:



S. 4,5,12:i:-

lowest highest

97


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Gallus gallus (fowl) - broilers - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]










2 3 0 3Trimethoprim


no

3

Antimicrobials:



S. Typhimurium

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




programmes

no

3

Antimicrobials:



S. Typhimurium

lowest highest


98


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Gallus gallus (fowl) - broilers - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

3

Antimicrobials:



S. Typhimurium

lowest highest

99


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Enteritidis in Gallus gallus (fowl) - broilers - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]





0.064 8 3 2 3 3Fluoroquinolones - Ciprofloxacin







no

8

Antimicrobials:



S. Enteritidis

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




programmes

no

8

Antimicrobials:



S. Enteritidis

lowest highest


100


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Enteritidis in Gallus gallus (fowl) - broilers - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

8

Antimicrobials:



S. Enteritidis

lowest highest

101


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Havana in Gallus gallus (fowl) - broilers - before slaughter - Control and eradication programmes- Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]


16 23 1 10 9 2 1 1Aminoglycosides - Streptomycin


0.5 23 2 6 5 10 2Cephalosporins - Cefotaxime

0.064 23 6 2 11 4 2 3 1Fluoroquinolones - Ciprofloxacin

8 23 5 2 3 3 9 1 5Penicillins - Ampicillin


256 23 6 1 6 9 1 1 5Sulfonamides

8 23 3 3 5 12 3Tetracyclines - Tetracycline



no

23

Antimicrobials:



S. Havana

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




programmes

no

23

Antimicrobials:



S. Havana

lowest highest


102


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Havana in Gallus gallus (fowl) - broilers - before slaughter - Control and eradication programmes- Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

23

Antimicrobials:



S. Havana

lowest highest

103


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Gallus gallus (fowl) - broilers - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]








256 8 1 1 1 3 2 1Sulfonamides




no

8

Antimicrobials:



Salmonella spp.

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




programmes

no

8

Antimicrobials:



Salmonella spp.

lowest highest


104


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Gallus gallus (fowl) - broilers - before slaughter - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim



programmes

no

8

Antimicrobials:



Salmonella spp.

lowest highest

105


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Gallus gallus (fowl) - breeding flocks, unspecified - adult - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]











Gallus gallus (fowl) - breeding flocks, unspecified - adult - Control and eradication programmes

no

7

Antimicrobials:



Salmonella spp.

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096


Gallus gallus(fowl) -

breeding flocks,unspecified -

adult - Controland eradication

programmes

no

7

Antimicrobials:



Salmonella spp.

lowest highest


106


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Gallus gallus (fowl) - breeding flocks, unspecified - adult - Control and eradicationprogrammes - Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]







8 1024Sulfonamides


0.25 32Trimethoprim

Gallus gallus(fowl) -

breeding flocks,unspecified -

adult - Controland eradication

programmes

no

7

Antimicrobials:



Salmonella spp.

lowest highest

107


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Enteritidis in Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes -Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]











Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes

no

3

Antimicrobials:



S. Enteritidis

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096



Gallus gallus(fowl) - layinghens - adult -Control anderadication

programmes

no

3

Antimicrobials:



S. Enteritidis

lowest highest


108


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Enteritidis in Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes -Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]






8 1024Sulfonamides


0.5 32Trimethoprim


programmes

no

3

Antimicrobials:



S. Enteritidis

lowest highest

109


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes -Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]










2 4 0 4Trimethoprim


no

4

Antimicrobials:



S. Typhimurium

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




programmes

no

4

Antimicrobials:



S. Typhimurium

lowest highest


110


Portugal - 2013

Table Antimicrobial susceptibility testing of S. Typhimurium in Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes -Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim


programmes

no

4

Antimicrobials:



S. Typhimurium

lowest highest

111


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes -Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]




0.5 21 0 7 12 2Cephalosporins - Cefotaxime




256 21 2 3 1 7 7 1 2Sulfonamides




no

21

Antimicrobials:



Salmonella spp.

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096




programmes

no

21

Antimicrobials:



Salmonella spp.

lowest highest


112


Portugal - 2013

Table Antimicrobial susceptibility testing of Salmonella spp. in Gallus gallus (fowl) - laying hens - adult - Control and eradication programmes -Suspect sampling - Official and industry sampling - animal sample - quantitative data [Dilution method]






8 1024Sulfonamides


0.25 32Trimethoprim


programmes

no

21

Antimicrobials:



Salmonella spp.

lowest highest

113


Portugal - 2013

Table Cut-off values for antibiotic resistance testing of Salmonella in Animals

Standard methods used for testing

NCCLS/CLSIEUCAST

EFSA 2Gentamicin

NON-EFSA 16

Aminoglycosides

Streptomycin

EFSA 16Amphenicols Chloramphenicol

EFSA 0.5Cefotaxime

EFSA 2

Cephalosporins

Ceftazidime

EFSA 0.064Fluoroquinolones Ciprofloxacin

EFSA 8Penicillins Ampicillin

EFSA 16Quinolones Nalidixic acid

EFSA 256Sulfonamides Sulfonamides

EFSA 8Tetracyclines Tetracycline

EFSA 2Trimethoprim Trimethoprim

Concentration (microg/ml) Zone diameter (mm)

Standard Resistant > Resistant <=

Test Method Used

Agar dilution

114


Portugal - 2013

115


Portugal - 2013

Table Cut-off values for antibiotic resistance testing of Salmonella in Feed


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

NON-EFSA 16

Aminoglycosides

Streptomycin


EFSA 0.5Cefotaxime

EFSA 2

Cephalosporins

Ceftazidime









Test Method Used

Agar dilution

116


Portugal - 2013

117


Portugal - 2013

Table Cut-off values for antibiotic resistance testing of Salmonella in Food


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

NON-EFSA 16

Aminoglycosides

Streptomycin


EFSA 0.5Cefotaxime

EFSA 2

Cephalosporins

Ceftazidime









Test Method Used

Agar dilution

The cut-off used for Streptomycine was appointed by the EURL-AR for ring trials and were used also for monitoring purposes.

Footnote:

118


Portugal - 2013

119


Portugal - 2013

Table Cut-off values for antibiotic resistance testing of Salmonella in Humans


EUCAST

14Gentamicin

18Kanamycin

16

Aminoglycosides

Streptomycin

17Amphenicols Chloramphenicol

22Cephalosporins 3rd generationcephalosporins

22Fluoroquinolones Ciprofloxacin

19Penicillins Ampicillin

18Quinolones Nalidixic acid

18Tetracyclines Tetracycline



Test Method Used

Disc diffusion


2.2 CAMPYLOBACTERIOSIS


Additional informationDiagnostic techniques:

Foodstuffs - Screening: VIDAS CAM. Confirmation: Internal method based on ISO 10272.- Typing of isolates by Lior method.

Other than foodstuffs:- Samples from sheathwashings, semen, intestinal scrapings and feces are plated in Campylobacteragar or Brucella agar supplemented with: SR 69, SR84, SR 85 (C. foetus), SR 117 (all from Oxoid) andselective media Campylosel (BiomÃ©rieux)and skirrow Campylobacter selective Agar (Merck).- Biochemical identification by API system.

A. Thermophilic Campylobacter general evaluation

120Portugal - 2013


2.2.2 Campylobacteriosis in humans

Reporting system in place for the human casesSentinel surveillance, estimated coverage 10%.

Case definitioncase is considered when meeting the clinical and laboratory criteria.

Diagnostic/analytical methods usedCulture and real - time PCR

Notification system in place Not notifiable disease

National evaluation of the recent situation, the trends and sources of infectionIncrease trend in erythromycin resistance

Relevance as zoonotic diseaseIn point studies, is the main aetiological agent of acute gastroenteritis.

A. Thermophilic Campylobacter in humans

121Portugal - 2013

122


Portugal - 2013

Table Campylobacter in humans - Species/serotype distribution

34 34 0 0C. coli

369 369 0 0C. jejuni

0 0 0 0C. upsaliensis


Autochthon Inc.

Importedcases

ImportedInc.

Unknownstatus


Campylobacter 403 0 403 0 0 0 0

123


Portugal - 2013

Table Campylobacter in humans - Age distribution

8 5 3 71 40 31 17 10 7<1 year

18 12 6 191 112 79 19 11 81 to 4 years

5 3 2 72 37 35 13 9 45 to 14 years

0 0 0 11 6 5 0 0 015 to 24 years

0 0 0 5 3 2 2 2 025 to 44 years

0 0 0 10 7 3 1 1 145 to 64 years

3 2 1 6 3 3 5 3 265 years and older

0 0 0 3 1 2 0 0 0Age unknown

34 22 12 369 209 160 57 36 22Total :

C. coli C. jejuni Campylobacter spp., unspecifiedAge distribution


124


Portugal - 2013

Table Campylobacter in humans - Seasonal distribution

3 16 0 2January

2 25 0 2February

3 24 0 5March

1 38 0 0April

1 29 0 3May

1 28 0 11June

6 37 0 8July

2 40 0 9August

3 19 0 7September

6 50 0 3October

3 37 0 6November

3 26 0 2December

34 369 0 58Total :

C. coli C. jejuniC.

upsaliensis

Campylobacter spp.,unspecifie

d


MonthsCases Cases Cases Cases

125


Portugal - 2013

2.2.3 Campylobacter in foodstuffs

Table Campylobacter in other food


Officialsampling

food sample> meat Domestic Single 25 Gram 23 1Meat from bovine animals - fresh - Processing plant

- Surveillance


Officialsampling




Officialsampling

food sample> meat Domestic Single 25 Gram 8 1 1Meat from goat - fresh - Processing plant -

Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 42 7 1Meat from pig - fresh - Processing plant -

Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 29 0Meat from pig - meat preparation - intended to be

eaten cooked - Processing plant - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 12 4 2Meat from sheep - fresh - Processing plant -

Surveillance


Officialsampling





weight Units tested

Total unitspositive for

Campylobacter

C. coli C. jejuni

1Meat from bovine animals - fresh - Processing plant- Surveillance

C. lari C. upsaliensis

ThermophilicCampylobact

er spp.,unspecified

Campylobacter spp.,

unspecified

126


Portugal - 2013

Table Campylobacter in other food


Meat from goat - fresh - Processing plant -Surveillance

6Meat from pig - fresh - Processing plant -Surveillance

Meat from pig - meat preparation - intended to beeaten cooked - Processing plant - Surveillance

2Meat from sheep - fresh - Processing plant -Surveillance




er spp.,unspecified

Campylobacter spp.,

unspecified

127


Portugal - 2013

Table Campylobacter in poultry meat


Officialsampling

food sample> meat Domestic Single 10 Gram 15 5Meat from broilers (Gallus gallus) - carcase -

Slaughterhouse - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 28 13 6 2Meat from broilers (Gallus gallus) - fresh -



Officialsampling

food sample> meat Domestic Single 25 Gram 18 8 5 3



Officialsampling

food sample> meat Domestic Single 25 Gram 6 2 1 1Meat from turkey - fresh - Processing plant -

Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 10 1 1




weight Units tested


Campylobacter

C. coli C. jejuni

5Meat from broilers (Gallus gallus) - carcase -Slaughterhouse - Surveillance

5Meat from broilers (Gallus gallus) - fresh -Processing plant - Surveillance


Meat from turkey - fresh - Processing plant -Surveillance



er spp.,unspecified

Campylobacter spp.,

unspecified

128


Portugal - 2013

Table Campylobacter in poultry meat




er spp.,unspecified

Campylobacter spp.,

unspecified

129


Portugal - 2013

2.2.4 Antimicrobial resistance in Campylobacter isolates

Table Antimicrobial susceptibility testing of Campylobacter in humans

120 2Aminoglycosides - Gentamicin

120 110Fluoroquinolones - Ciprofloxacin

120 17Macrolides - Erythromycin

0 0Penicillins - Ampicillin


120 85Tetracyclines - Tetracycline

120 7Fully sensitive

120 28Resistant to 1 antimicrobial

120 68Resistant to 2 antimicrobials



0 0Resistant to >4 antimicrobials

Campylobacterspp.,

unspecified

yes

1350

Antimicrobials:



Campylobacter

N n

130


Portugal - 2013

Table Antimicrobial susceptibility testing of C. coli in Meat from broilers (Gallus gallus) - carcase - chilled - Cutting plant - Surveillance - Objectivesampling - Official sampling - food sample - meat - quantitative data [Dilution method]


4 11 4 1 4 2 3 1Aminoglycosides - Streptomycin


16 11 11 1 8 1 1Quinolones - Nalidixic acid


8 11 8 1 2 8Macrolides - Erythromycin

Meat from broilers (Gallus gallus) - carcase - chilled - Cutting plant - Surveillance

no

11

Antimicrobials:



C. coli

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096


0.5 32Aminoglycosides - Streptomycin




0.5 64Macrolides - Erythromycin

Meat frombroilers (Gallus

gallus) -carcase -

chilled - Cuttingplant -

Surveillance

no

11

Antimicrobials:



C. coli

lowest highest


131


Portugal - 2013

Table Antimicrobial susceptibility testing of C. coli in Meat from broilers (Gallus gallus) - carcase - chilled - Cutting plant - Surveillance - Objectivesampling - Official sampling - food sample - meat - quantitative data [Dilution method]

132


Portugal - 2013

Table Antimicrobial susceptibility testing of C. coli in Meat from sheep - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]






8 1 0 1Macrolides - Erythromycin

Meat from sheep - fresh - chilled - Surveillance

no

1

Antimicrobials:



C. coli

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096







Meat fromsheep - fresh -

chilled -Surveillance

no

1

Antimicrobials:



C. coli

lowest highest


133


Portugal - 2013

Table Antimicrobial susceptibility testing of C. coli in Meat from sheep - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]

134


Portugal - 2013

Table Antimicrobial susceptibility testing of C. coli in Meat from turkey - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]







Meat from turkey - fresh - chilled - Surveillance

no

2

Antimicrobials:



C. coli

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096







Meat fromturkey - fresh -


no

2

Antimicrobials:



C. coli

lowest highest


135


Portugal - 2013

Table Antimicrobial susceptibility testing of C. coli in Meat from turkey - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]

136


Portugal - 2013

Table Antimicrobial susceptibility testing of C. jejuni in Meat from goat - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]





1Tetracyclines - Tetracycline


Meat from goat - fresh - chilled - Surveillance

no

1

Antimicrobials:



C. jejuni

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096







Meat from goat- fresh - chilled- Surveillance

no

1

Antimicrobials:



C. jejuni

lowest highest


137


Portugal - 2013

Table Antimicrobial susceptibility testing of C. jejuni in Meat from goat - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]

138


Portugal - 2013

Table Antimicrobial susceptibility testing of C. jejuni in Meat from broilers (Gallus gallus) - fresh - chilled - Surveillance - Objective sampling -Official sampling - food sample - meat - quantitative data [Dilution method]



0.5 5 4 1 1 1 2Fluoroquinolones - Ciprofloxacin



4 5 3 2 3Macrolides - Erythromycin

Meat from broilers (Gallus gallus) - fresh - chilled - Surveillance

no

5

Antimicrobials:



C. jejuni

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096







Meat frombroilers (Gallusgallus) - fresh -


no

5

Antimicrobials:



C. jejuni

lowest highest


139


Portugal - 2013

Table Antimicrobial susceptibility testing of C. jejuni in Meat from broilers (Gallus gallus) - fresh - chilled - Surveillance - Objective sampling -Official sampling - food sample - meat - quantitative data [Dilution method]

140


Portugal - 2013

Table Antimicrobial susceptibility testing of C. jejuni in Meat from turkey - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]







Meat from turkey - fresh - chilled - Surveillance

no

1

Antimicrobials:



C. jejuni

Cut-offvalue N n <=0.002 <=0.004 0.008 0.015 0.016 0.03 0.06 0.12 0.25 0.5 1 2 4 8 16 32 64 128 256 512 1024 2048 >4096







Meat fromturkey - fresh -


no

1

Antimicrobials:



C. jejuni

lowest highest


141


Portugal - 2013

Table Antimicrobial susceptibility testing of C. jejuni in Meat from turkey - fresh - chilled - Surveillance - Objective sampling - Official sampling -food sample - meat - quantitative data [Dilution method]

142


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. coli in Animals


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

EFSA 4

Aminoglycosides

Streptomycin


EFSA 8Macrolides Erythromycin





Test Method Used

Agar dilution

143


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. coli in Feed


2Gentamicin

4

Aminoglycosides

Streptomycin

0.5Fluoroquinolones Ciprofloxacin

8Macrolides Erythromycin





Test Method Used

144


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. coli in Food


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

EFSA 4

Aminoglycosides

Streptomycin







Test Method Used

Agar dilution

145


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. coli in Humans


NCCLS/CLSIEUCAST

12Aminoglycosides Gentamicin






Test Method Used

Disc diffusion

146


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. jejuni in Animals


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

EFSA 4

Aminoglycosides

Streptomycin







Test Method Used

Agar dilution

147


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. jejuni in Feed


2Gentamicin

4

Aminoglycosides

Streptomycin







Test Method Used

148


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. jejuni in Food


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

EFSA 4

Aminoglycosides

Streptomycin







Test Method Used

Agar dilution

149


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of C. jejuni in Humans


NCCLS/CLSIEUCAST

12Aminoglycosides Gentamicin






Test Method Used

Disc diffusion


2.3 LISTERIOSIS


Additional information* The searching of Listeria started on 1996 for raw milk and milk cheese (Portaria nÂº 533/93 from 21st ofMay has been updated by Portaria 56/96).

Diagnostic tecnhiques:

Foodstuffs/Feedingstuffs -Screening: VIDAS LMO2 (AFNOR validation). Detection:ISO 11290-1 (1996)and Amendment 1 (2004). Enumeration: ISO 11290-2 (1998) and Amendment 1 (2004).

Other than foodstuffs - Internal method - culture on:- Palcam agar, Oxford agar and Blood agar.- Biochemical reactions by API Coryne or API Listeria strips.

A. Listeriosis general evaluation

150Portugal - 2013

151


Portugal - 2013

2.3.2 Listeriosis in humans

Table Listeria in humans - Species/serotype distribution

4L. monocytogenes - L. monocytogenesserovar 1/2b

2L. monocytogenes - L.monocytogenes, unspecified

3L. monocytogenes - L. monocytogenesserovar 1/2a

18L. monocytogenes - L. monocytogenesserovar 4b

Cases Cases Inc.Species/serotype Distribution

Listeria 27 0

152


Portugal - 2013

Table Listeria in humans - Age distribution

1 1 015 to 24 years

1 0 125 to 44 years

7 3 445 to 64 years

12 7 565 years and older

6 3 3Age unknown

27 14 13 0 0 0Total :

L. monocytogenes Listeria spp., unspecifiedAge distribution

All M F All M F

153


Portugal - 2013

2.3.3 Listeria in foodstuffs

Table Listeria monocytogenes in milk and dairy products


Officialsampling food sample Domestic Single 25 Gram 155 75 155 75

Cheeses made from cows' milk - soft and semi-soft -made from raw or low heat-treated milk - Processingplant - Surveillance


Officialsampling

food sample> milk Domestic Single 25 Gram 7 0 7 0



Officialsampling

animalsample Domestic Batch 25 Gram 20 0



Officialsampling

animalsample Domestic Batch 25 Gram 5 0Cheeses made from cows' milk - hard - made from

pasteurised milk - Retail - Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 5 0 5 0

Cheeses made from goats' milk - soft and semi-soft -made from raw or low heat-treated milk - Processingplant - Surveillance


Officialsampling




Officialsampling




Officialsampling


Cheeses made from goats' milk - soft and semi-soft -made from pasteurised milk - Retail - Surveillance



weight Units tested

Total unitspositive for L.monocytogen

es

Units testedwith detection

method

Listeriamonocytogenes presence

in x g

154


Portugal - 2013



Officialsampling




HACCP andown checks




Officialsampling


Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk - Retail- Surveillance


Officialsampling




Officialsampling


Cheeses made from sheep's milk - hard - made fromraw or low heat-treated milk - Processing plant -Surveillance


Officialsampling




Officialsampling




Officialsampling




Officialsampling

animalsample Domestic Batch 25 Gram 75 0Cheeses made from cows' milk - fresh - made from




weight Units tested


es


method


in x g

155


Portugal - 2013



Officialsampling


Cheeses made from cows' milk - unspecified -made from pasteurised milk - Retail - Surveillance


Officialsampling




Officialsampling




Officialsampling

animalsample Domestic Batch 25 Gram 35 0Cheeses made from goats' milk - fresh - made from



Officialsampling


Cheeses made from goats' milk - unspecified -made from pasteurised milk - Retail - Surveillance


Officialsampling

food sample> milk Domestic Single 25 Gram 18 0 18 0Cheeses made from sheep's milk - fresh -



Officialsampling


Cheeses made from sheep's milk - fresh - madefrom pasteurised milk - Processing plant -Surveillance


Officialsampling




Officialsampling




Officialsampling


Cheeses made from sheep's milk - unspecified -made from raw or low heat-treated milk - Processingplant - Surveillance



weight Units tested


es


method


in x g

156


Portugal - 2013



Officialsampling




Officialsampling




Officialsampling




Officialsampling


Cheeses, made from mixed milk from cows, sheepand/or goats - fresh - made from pasteurised milk -Retail - Surveillance


Officialsampling




Officialsampling




Officialsampling


Cheeses, made from mixed milk from cows, sheepand/or goats - soft and semi-soft - made frompasteurised milk - Retail - Surveillance


Officialsampling





weight Units tested


es


method


in x g

157


Portugal - 2013



Officialsampling




Officialsampling




Officialsampling




Officialsampling




Officialsampling




Officialsampling


Dairy products (excluding cheeses) - ice-cream -made from pasteurised milk - Retail - Surveillance



weight Units tested


es


method


in x g

Cheeses made from cows' milk - soft and semi-soft -made from raw or low heat-treated milk - Processingplant - Surveillance

Units testedwith

enumerationmethod

> detectionlimit but <=100 cfu/g

L.monocytogen

es > 100cfu/g

158


Portugal - 2013


7 0 0Cheeses made from cows' milk - soft and semi-soft -made from pasteurised milk - Processing plant -Surveillance

20 0Cheeses made from cows' milk - soft and semi-soft -made from pasteurised milk - Retail - Surveillance

5 0Cheeses made from cows' milk - hard - made frompasteurised milk - Retail - Surveillance

Cheeses made from goats' milk - soft and semi-soft -made from raw or low heat-treated milk - Processingplant - Surveillance

55 0Cheeses made from goats' milk - soft and semi-soft -made from raw or low heat-treated milk - Retail -Surveillance

5 0 0Cheeses made from goats' milk - soft and semi-soft -made from pasteurised milk - Processing plant -Surveillance

5 0Cheeses made from goats' milk - soft and semi-soft -made from pasteurised milk - Retail - Surveillance

1 0 0Cheeses made from goats' milk - hard - made fromraw or low heat-treated milk - Processing plant -Surveillance

65 0 0Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk -Processing plant - Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

159


Portugal - 2013


260 2 3Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk - Retail- Surveillance

4 0 0Cheeses made from sheep's milk - soft and semi-soft - made from pasteurised milk - Processing plant- Surveillance

Cheeses made from sheep's milk - hard - made fromraw or low heat-treated milk - Processing plant -Surveillance

5 0Cheeses made from sheep's milk - hard - made fromraw or low heat-treated milk - Retail - Surveillance


5 0 0Cheeses made from cows' milk - fresh - made frompasteurised milk - Processing plant - Surveillance

75 0Cheeses made from cows' milk - fresh - made frompasteurised milk - Retail - Surveillance

60 0Cheeses made from cows' milk - unspecified -made from pasteurised milk - Retail - Surveillance


Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

160


Portugal - 2013


12 0 0Cheeses made from goats' milk - fresh - made frompasteurised milk - Processing plant - Surveillance

35 0Cheeses made from goats' milk - fresh - made frompasteurised milk - Retail - Surveillance

10 0Cheeses made from goats' milk - unspecified -made from pasteurised milk - Retail - Surveillance

18 0 0Cheeses made from sheep's milk - fresh -Processing plant - Surveillance



48 0 0Cheeses made from sheep's milk - soft and semi-soft - made from raw or low heat-treated milk -Processing plant - Surveillance

Cheeses made from sheep's milk - unspecified -made from raw or low heat-treated milk - Processingplant - Surveillance

15 0Cheeses made from sheep's milk - unspecified -made from raw or low heat-treated milk - Retail -Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

161


Portugal - 2013



8 0 0


15 0Cheeses, made from mixed milk from cows, sheepand/or goats - fresh - made from pasteurised milk -Retail - Surveillance


4 0 0


10 0Cheeses, made from mixed milk from cows, sheepand/or goats - soft and semi-soft - made frompasteurised milk - Retail - Surveillance


6 0 0


Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

162


Portugal - 2013


5 0


10 0


5 0Cheeses, made from unspecified milk or otheranimal milk - hard - made from raw or low heat-treated milk - Retail - Surveillance

20 0Cheeses, made from unspecified milk or otheranimal milk - soft and semi-soft - made from raw orlow heat-treated milk - Retail - Surveillance

140 0Dairy products (excluding cheeses) - ice-cream -made from pasteurised milk - Retail - Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

163


Portugal - 2013

Table Listeria monocytogenes in other foods


Officialsampling


Meat from broilers (Gallus gallus) - meat products -cooked, ready-to-eat - Processing plant -Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 104 4 104 4Meat from pig - meat products - cooked, ready-to-



Officialsampling food sample Domestic Single 25 Gram 91 15 91 15Meat from pig - meat products - cooked, ready-to-

eat - Retail - Surveillance


Officialsampling food sample Domestic Single 25 Gram 50 7 50 7Meat from bovine animals - fresh - Processing plant

- Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 5 0Molluscan shellfish - cooked - Retail - Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 15 0Vegetables - pre-cut - ready-to-eat - Retail -

Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 70 0Bakery products - cakes - containing heat-treated

cream - Retail - Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 15 0Crustaceans - prawns - cooked - chilled - Retail -

Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 60 0Crustaceans - prawns - raw - frozen - Retail -

Surveillance


Officialsampling

animalsample Domestic Single 25 Gram 10 0 10 0Crustaceans - unspecified - cooked - Border



Officialsampling

animalsample Domestic Single 25 Gram 8 0 8 0Crustaceans - unspecified - cooked - chilled -



Officialsampling

animalsample Domestic Single 25 Gram 15 0 15 0Fishery products, unspecified - cooked - Border




weight Units tested


es


method


in x g

164


Portugal - 2013



Officialsampling

animalsample Domestic Single 25 Gram 2 0 2 0Fishery products, unspecified - ready-to-eat -



HACCP andown checks

animalsample Domestic Single 25 Gram 10 0 10 0

Foodstuffs intended for special nutritional uses -dried dietary foods for special medical purposesintended for infants below 6 months - Processingplant - Surveillance


HACCP andown checks

animalsample Domestic Single 25 Gram 72 0 72 0Fruits - pre-cut - ready-to-eat - Catering -

Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 40 0Fruits - products - dried - Retail - Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 5 0Fruits and vegetables - pre-cut - ready-to-eat -



HACCP andown checks

food sample> milk Domestic Single 25 Gram 18 0 18 0Infant formula - liquid - Hospital or medical care



Officialsampling




Officialsampling

animalsample Domestic Batch 25 Gram 5 0Meat from pig - meat products - cooked, ready-to-

eat - chilled - Retail - Surveillance


Officialsampling


Meat from pig - meat products - raw but intended tobe eaten cooked - Processing plant - Surveillance


Officialsampling




Officialsampling

animalsample Domestic Batch 25 Gram 20 0Meat from pig - meat products - raw ham - Retail -

Surveillance



weight Units tested


es


method


in x g

165


Portugal - 2013



Officialsampling

animalsample Domestic Batch 25 Gram 110 0Meat from pig - meat products - unspecified, ready-

to-eat - Retail - Surveillance


Officialsampling

food sample> meat Domestic Single 25 Gram 12 0 12 0Meat from turkey - meat products - cooked, ready-to

-eat - Processing plant - Surveillance


Officialsampling




Officialsampling

food sample> meat Domestic Single 25 Gram 3 0 3 0Meat, mixed meat - meat products - cooked, ready-

to-eat - Processing plant - Surveillance


Officialsampling

animalsample Domestic Single 25 Gram 20 0 20 0Molluscan shellfish - cooked - Border inspection

activities - Surveillance


HACCP andown checks

animalsample Domestic Single 25 Gram 33 0 33 0Other processed food products and prepared dishes

- sandwiches - Catering - Surveillance


Officialsampling


Other processed food products and prepared dishes- sandwiches - non-meat - Retail - Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 130 3Other processed food products and prepared dishes

- sushi - Retail - Surveillance


Officialsampling


Other processed food products and prepared dishes- unspecified - non-ready-to-eat foods - chilled -Retail - Surveillance


HACCP andown checks

animalsample Domestic Single 25 Gram 924 19 924 19



HACCP andown checks

animalsample Domestic Single 25 Gram 147 3 147 3Ready-to-eat salads - Catering - Surveillance


Officialsampling

animalsample Domestic Batch 25 Gram 35 0Ready-to-eat salads - Retail - Surveillance



weight Units tested


es


method


in x g

166


Portugal - 2013



Officialsampling

animalsample Domestic Batch 25 Gram 10 0Vegetables - products - Retail - Surveillance



weight Units tested


es


method


in x g

8 0 0Meat from broilers (Gallus gallus) - meat products -cooked, ready-to-eat - Processing plant -Surveillance

104 0 0Meat from pig - meat products - cooked, ready-to-eat - Processing plant - Surveillance

Meat from pig - meat products - cooked, ready-to-eat - Retail - Surveillance

Meat from bovine animals - fresh - Processing plant- Surveillance

5 0Molluscan shellfish - cooked - Retail - Surveillance

15 0Vegetables - pre-cut - ready-to-eat - Retail -Surveillance

70 0Bakery products - cakes - containing heat-treatedcream - Retail - Surveillance

15 0Crustaceans - prawns - cooked - chilled - Retail -Surveillance

60 0Crustaceans - prawns - raw - frozen - Retail -Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

167


Portugal - 2013


10 0 0Crustaceans - unspecified - cooked - Borderinspection activities - Surveillance

8 0 0Crustaceans - unspecified - cooked - chilled -Processing plant - Surveillance

15 0 0Fishery products, unspecified - cooked - Borderinspection activities - Surveillance

2 0 0Fishery products, unspecified - ready-to-eat -Processing plant - Surveillance

Foodstuffs intended for special nutritional uses -dried dietary foods for special medical purposesintended for infants below 6 months - Processingplant - Surveillance

72 0 0Fruits - pre-cut - ready-to-eat - Catering -Surveillance

40 0Fruits - products - dried - Retail - Surveillance

5 0Fruits and vegetables - pre-cut - ready-to-eat -Retail - Surveillance

18 0 0Infant formula - liquid - Hospital or medical carefacility - Surveillance

1 0 0Meat from broilers (Gallus gallus) - meat preparation- intended to be eaten cooked - Processing plant -Surveillance

5 0Meat from pig - meat products - cooked, ready-to-eat - chilled - Retail - Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

168


Portugal - 2013


5 0 0Meat from pig - meat products - raw but intended tobe eaten cooked - Processing plant - Surveillance

25 2Meat from pig - meat products - raw but intended tobe eaten cooked - chilled - Retail - Surveillance

20 0Meat from pig - meat products - raw ham - Retail -Surveillance

110 0Meat from pig - meat products - unspecified, ready-to-eat - Retail - Surveillance

12 0 0Meat from turkey - meat products - cooked, ready-to-eat - Processing plant - Surveillance

1 0 0Meat, mixed meat - meat preparation - intended tobe eaten cooked - Processing plant - Surveillance

3 0 0Meat, mixed meat - meat products - cooked, ready-to-eat - Processing plant - Surveillance

20 0 0Molluscan shellfish - cooked - Border inspectionactivities - Surveillance

33 0 0Other processed food products and prepared dishes- sandwiches - Catering - Surveillance

50 0Other processed food products and prepared dishes- sandwiches - non-meat - Retail - Surveillance

130 2 1Other processed food products and prepared dishes- sushi - Retail - Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g

169


Portugal - 2013


45 0Other processed food products and prepared dishes- unspecified - non-ready-to-eat foods - chilled -Retail - Surveillance

924 19 0Other processed food products and prepared dishes- unspecified - ready-to-eat foods - Catering -Surveillance

147 3 0Ready-to-eat salads - Catering - Surveillance

35 0Ready-to-eat salads - Retail - Surveillance

10 0Vegetables - products - Retail - Surveillance

Units testedwith

enumerationmethod


L.monocytogen

es > 100cfu/g


2.4 E. COLI INFECTIONS


Additional informationAt LNIV the following procedures are performed in E. coli isolates of cattle, swine, sheep and goats(strains that are serotyped).At poultry isolates, serotyping is not being done.

Diagnostic Techniques:Internal method.1 - Culture:

Platting in: Tryptose Blood AgarMacConkey AgarMinca Agar

To different E. coli colonies, the following biochemical reactions are done:Simmons CitrateMR-VPAdonitolDulcitolInositolMannitolSorbitolGlucoseSucroseRaffinoseMalonateUrease

2 - Serology:Serotyping by searching somatic (O) and capsular (K) antigens.

3 - Searching of enterotoxins:- ST (by PCR)- LT (by Biken test, CHO cells and PCR)

4 - Searching of citotoxins:- in Vero and HeLa cells.

5 - Adesin detection:- F5, F6, F41

6 - Antibiotic susceptibility testing

A. Verotoxigenic Escherichia coli infections general evaluation

170Portugal - 2013


171Portugal - 2013


2.4.2 E. coli infections in humans


Diagnostic/analytical methods usedPCR and serotyping

Notification system in placeNot a notifiable disease


Relevance as zoonotic diseaseMajor

A. Verotoxigenic Escherichia coli infections in humans

172Portugal - 2013

173


Portugal - 2013

Table Escherichia coli, pathogenic in humans - Species/serotype distribution

0 0 0 0 0 0HUS

0 0 0 0 0 0- clinical cases

0 0 0 0 0 0- lab. confirmed cases

0 0 0 0 0- caused by O157 (VT+)

0 0 0 0 0 0- caused by other VTEC

2 0 2 0 0 0E.coli infect. (except HUS)

2 0 2 0 0 0- clinical cases

2 0 2 0 0 0- laboratory confirmed

0 0 0 0 0 0- caused by 0157 (VT+)

2 0 2 0 0 0- caused by other VTEC


Autochthon Inc.

Importedcases

ImportedInc.


Escherichia coli, pathogenic

174


Portugal - 2013

Table Escherichia coli, pathogenic in humans - Age distribution

0 0 0 0 0 0 0 0 0<1 year

1 1 0 0 0 0 1 0 11 to 4 years

0 0 0 0 0 0 0 0 05 to 14 years

0 0 0 0 0 0 0 0 015 to 24 years

0 0 0 0 0 0 0 0 025 to 44 years

1 1 0 0 0 0 1 0 145 to 64 years

0 0 0 0 0 0 0 0 065 years and older

0 0 0 0 0 0 0 0 0Age unknown

2 2 0 0 0 0 2 0 2Total :

Verotoxigenic E. coli (VTEC) Verotoxigenic E. coli (VTEC) -VTEC O157:H7

Verotoxigenic E. coli (VTEC) -VTEC non-O157

Age distribution

All M F All M F All F M

175


Portugal - 2013

2.4.3 Escherichia coli, pathogenic in foodstuffs

Table VT E. coli in food


Officialsampling

food sample> meat Domestic ISO

16654:2001 Single 25 Gram 15 1 1Meat from bovine animals - meat preparation -intended to be eaten cooked - Processing plant -Surveillance


Officialsampling


16654:2001 Single 25 Gram 13 1 1Meat from bovine animals - minced meat - intendedto be eaten cooked - Processing plant - Surveillance


Officialsampling


16654:2001 Single 25 Gram 34 9 9Meat from pig - meat preparation - intended to beeaten cooked - Processing plant - Surveillance


Officialsampling


16654:2001 Single 25 Gram 6 2 2Meat from pig - minced meat - intended to be eatencooked - Processing plant - Surveillance


Officialsampling


16654:2001 Single 25 Gram 10 1 1Meat, mixed meat - meat preparation - intended tobe eaten cooked - Processing plant - Surveillance


Officialsampling


16654:2001 Batch 25 Gram 5 0Meat, mixed meat - meat preparation - intended tobe eaten cooked - Retail - Surveillance


Officialsampling food sample Domestic PCR Batch 25 Gram 5 1 1Vegetables - non-pre-cut - Processing plant -

Surveillance


HACCP andown checks food sample Domestic ELISA Single 25 Gram 6 0Vegetables - pre-cut - Processing plant -

Surveillance


Samplingstrategy Sampler Sample type Sample origin Analytical

MethodSampling unit Sample

weight Units tested


VerotoxigenicE. coli

(VTEC)


(VTEC) -VTEC O157

176


Portugal - 2013

Table VT E. coli in food


Meat from bovine animals - minced meat - intendedto be eaten cooked - Processing plant - Surveillance

Meat from pig - meat preparation - intended to beeaten cooked - Processing plant - Surveillance

Meat from pig - minced meat - intended to be eatencooked - Processing plant - Surveillance


Meat, mixed meat - meat preparation - intended tobe eaten cooked - Retail - Surveillance


Vegetables - pre-cut - Processing plant -Surveillance


(VTEC) -VTEC non-

O157


(VTEC) -VTEC,

unspecified


2.5 TUBERCULOSIS, MYCOBACTERIAL DISEASES


2.5.2 Tuberculosis, mycobacterial diseases in humans

Reporting system in place for the human casesInstituto Nacional de Saúde Dr. Ricardo Jorge

A. Tuberculosis due to Mycobacterium bovis in humans

177Portugal - 2013

178


Portugal - 2013

Table Mycobacterium in humans - Species/serotype distribution

0M. bovis

2292M. tuberculosis


Autochthon Inc.

Importedcases

ImportedInc.


Mycobacterium 2292 0 0 0 0 0

179


Portugal - 2013

Table Mycobacterium in humans - Age distribution

0 0 0<1 year

0 0 01 to 4 years

0 0 05 to 14 years

0 0 015 to 24 years

0 0 025 to 44 years

0 0 045 to 64 years


0 0 0Age unknown

0 0 0Total :

M. bovisAge distribution

All M F


2.5.3 Mycobacterium in animals

Status as officially free of bovine tuberculosis during the reporting yearFree regions

At mainland, the Algarve region was recognized as Officially Free of Bovine Tuberculosis according toComission Decision 2012/204/UE of 19th April.


Tuberculosis testing is performed in all bovine, older than 6 weeks of age, using the intra-dermalcomparative test.

The herds are classified and sampled according to Council Directive 64/432/EEC and National Dec. Lei nº272/2000, November 8th.

Frequency of the samplingThe herds are classified and sampled according to Council Directive 64/432/EEC and National Dec. Lei nº272/2000, November 8th.

Type of specimen takenintra-dermal comparative test, blood (gama-IFN), organs

Diagnostic/analytical methods usedThe National Reference Laboratory (NRL) is Instituto Nacional de Investigação Agrária e Veterinária, I. P(INIAV).

Diagnostic techniques:

- Internal method.

- direct smear

- solid media: stonebrink and Lowenstein-Jensen.

- liquid media: bactec.

The classification of Mycobacterium is based on: BM techniques.

INIAV is responsible for the Mycobacterium isolation on the tuberculin reactors animals and others,

A. Mycobacterium bovis in bovine animals

180Portugal - 2013


following the procedures above mentioned.

Vaccination policyVaccination is forbidden.

Other preventive measures than vaccination in placePre-movement tests are mandatory for breeding animals.


An Eradication Plan for Bovine Tuberculosis is carried out and supervised by DGAV.

Measures in case of the positive findings or single cases- Herd under official restrictions;

- Isolation of suspected or infected animals in the herd;

- Positive animals compulsory slaughtered, under official supervision, with sample collection for laboratorydiagnosis;

- Animal movements are forbiden from and to the herd;

- Desinfection of all premises, equipment and materials;

- Testing of all remaining animals;

- Thermic treatment of the milk.

Notification system in placeTuberculosis is a notifiable disease.

181Portugal - 2013



There is no national surveillance plan in place, but there is target surveillance in certain areas.

Sampling collection is done during hunting and all carcasses intended to human consumption undergo apost-mortem examination.

Vaccination policyVaccination is forbiden

Notification system in placeTuberculosis is a notifiable disease in all species.

B. Mycobacterium bovis in farmed deer

182Portugal - 2013

183


Portugal - 2013

Table Bovine tuberculosis - data on herds - Community co-financed eradication programmes

Comments:1) 0

Total number ofherds

Total number ofherds under the

programme

Number ofherds checked

Number ofpositive herds

Number of newpositive herds

Number ofherds

depopulated

% positive herdsdepopulated

Indicators

% herdcoverage

% positive herdsPeriod herdprevalence

% new positiveherds HerdIncidenceRegion

41098 30965 28508 98 76 2 2.04 92.07 .34 .27Continente

9435 7093 2288 10 10 0 0 32.26 .44 .44Região Autónoma dosAçores

50533 38058 30796 108 86 2 1.85 80.92 .35 .28Total :1)

If present, the row "Total -1" refers to analogous data of the previous year.

184


Portugal - 2013

Table Bovine tuberculosis - data on animals - Community co-financed eradication programmes

Comments:1) 0

Total number ofanimals

Number ofanimals to be

tested under theprogramme

Number ofanimals tested


individually

Number ofpositive animals

Indicators

% coverage atanimal level

% positiveanimals - animal

prevalenceRegion

Slaughtering

Number ofanimals with

positive resultslaughtered or

culled


slaughtered

1254670 1126083 1012725 867524 491 508 585 89.93 .05Continente

250306 200788 77783 75907 10 9 9 38.74 .01Região Autónoma dosAçores

1504976 1326871 1090508 943431 501 517 594 82.19 .05Total :1)


185


Portugal - 2013

Table Bovine tuberculosis - data on status of herds at the end of the period - Community co-financed eradication programmes

Comments:1) 0

Animals Herds Animals Herds

Not free or not officially free

Animals HerdsRegion

Total number of herds andanimals under the

programme

Animals Herds Animals Herds AnimalsHerds AnimalsHerds

Unknown Free Officially freeFree or officially freesuspended Free Officially free

Last check positive Last check negative

Status of herds and animals under the programme

14 1919 78 12046 110 3742 30763 1108376Continente

14 1919 78 12046 132 5308 37834 1307598Portugal

0 0 0 0 22 1566 7071 199222Região Autónoma dosAçores

0 0 0 0 28 3838 156 24092 264 10616 0 0 75668 2615196Total :1)


186


Portugal - 2013

Table Bovine tuberculosis in countries and regions that do not receive Community co-financing for eradication programmes

Comments:1) 0

Herds Animals Number of herds % Number of herds %

Number oftuberculin tests

carried out beforethe introductioninto the herds

(Annex A(I)(2)(c)third indent (1) of

Directive64/432/EEC)

Number ofanimals withsuspiciouslesions of

tuberculosisexamined andsubmitted to

histopathologicaland

bacteriological

Number ofanimals detected

positive inbacteriologicalexamination

Total number of existing bovine Infected herdsOfficially free herds

Interval betweenroutine tuberculin

tests


Routine tuberculin testing

Region

335 11093 335 100 0 0 every 48 months 1068 31 0 0Algarve

335Portugal

335 11093 670 200 0 0 0 1068 31 0 0Total :1)



2.6 BRUCELLOSIS


Additional informationFoodstuffsBrucella isolation:- Samples are plated in 6 petri dishes of FarrelÂ´s medium (3 incubated in CO2 atmosphere (CO2) andthe others are incubated at normal atmosphere (N));- Incubation at 37 degrees Celsius (+-1 degree celsius) for 10 days;- 1st reading of the plates on the 4/5th day of incubation;- 2nd reading on the 10th day of incubation;- Suspected colonies are streaked on 2 agar slopes (one for (CO2) and the other for (N)for typing.

Brucella typing:- Biochemical tests (urease, catalase and oxidase);- CO2 requirement;- H2S production;- Dye sensitivity (Thionin, Basic Fucsin and Safrinin O);- Agglutination with acriflavine and monospecific A and M antisera;- Lysis by phages;- Differentiation of vaccine and field strains.

For each set of plating and typing, reference strains are used.

A. Brucellosis general evaluation

187Portugal - 2013

188


Portugal - 2013

2.6.2 Brucellosis in humans

Table Brucella in humans - Age distribution

1 1 05 to 14 years

1 1 0 0 015 to 24 years

8 5 325 to 44 years

4 4 045 to 64 years


1 1 0Age unknown

0 0 0 1 1 0 19 13 6Total :

B. abortus B. melitensis Brucella spp., unspecifiedAge distribution


189


Portugal - 2013

2.6.3 Brucella in foodstuffs

Table Brucella in food


Officialsampling

food sample> milk Domestic Single 5 0



Officialsampling




Officialsampling




Officialsampling




Officialsampling

food sample> milk Domestic Single 6 0Milk, cows' - raw milk - Farm - Surveillance


Officialsampling

food sample> milk Domestic Single 7 0Milk, goats' - raw milk - Farm - Surveillance


Officialsampling

food sample> milk Domestic Single 14 0Milk, sheep's - raw milk - Farm - Surveillance


Samplingstrategy Sampler Sample type Sample origin Sampling unit Units tested


BrucellaB. abortus B. melitensis B. suis

190


Portugal - 2013

Table Brucella in food





Milk, cows' - raw milk - Farm - Surveillance

Milk, goats' - raw milk - Farm - Surveillance

Milk, sheep's - raw milk - Farm - Surveillance

Brucella spp.,unspecified


2.6.4 Brucella in animals

Status as officially free of bovine brucellosis during the reporting yearFree regions

In the Açores, there are 6 islands (Santa Maria, Faial, Graciosa, Pico, Flores and Corvo) that are OfficiallyFree of Bovine Brucellosis , according to Comission Decisions 2002/588/CE of 11th July 2002 and2009/600/CE of 5th August.At mainland, the Algarve region was recognized as Officially Free of Bovine Brucellosis according toComission Decision 2012/204/UE of 19th April.


Serology is performed in cattle older than 12 months of age.

The herds are classified and sampled according to Council Directive 64/432/EEC and Decreto-Leinº244/2000 ( Sep. 27th ).

Frequency of the samplingThe herds are sampled according to Council Directive 64/432/EEC and Decreto-Lei nº244/2000 ( Sep.27th ) for cattle, sheep and goats.

Type of specimen takenBlood, milk, organs,vaginal mucus, semen,aborted foetus, placenta.

Diagnostic/analytical methods usedThe National Reference Laboratory (NRL) is Instituto Nacional de Investigação Agrária e Veterinária, I. P(INIAV).


Serology:

- Rose Bengal Test (RBT);

- Complement Fixation Test (CFT);

If RBT is positive CFT is performed.

Bacteriology - Samples from:

- live animals (milk, vaginal mucus, semen, aborted foetus, placenta;

A. Brucella abortus in bovine animals

191Portugal - 2013


- dead animals (liver, spleen, lymph nodes, udder and uterus)

are plated in Farrel medium (Difco Tryptose Agar + SR209 Oxoid supplement + 5% horse serum).

- Biochemical reactions (urease, catalase and oxidase).

Typing of isolates:

- CO2 requirement;

- H2S production;

- Agglutination with monospecific antisera ( anti-A, anti-M and anti-R or acriflavine test);

- Growth on dyes:

1/50.000 and 1/100.000 of basic fucsin

1/50.000 and 1/100.000 of thionin.

- Lysis by phages;

- Differentiation of vaccine and field strains.

Vaccination policyVaccination is forbidden but if an exceptional sanitary situation occurs, vaccination can be allowed withspecific protocols between the National Veterinary Authority, the Regional Veterinary Authority and theowner(s) of the cattle.

Other preventive measures than vaccination in placePre-movement tests are mandatory for breeding animals.


An Eradication Plan for cattle is carried out and supervised by DGAV.

Measures in case of the positive findings or single casesSuspected Herd:

- Herd under oficial surveillance;

- Epidemiological questionaire;


192Portugal - 2013


- Isolation of suspected animals in the herd;

- Sample collection for laboratory diagnosis.

Positive Herd:

- Herd under official restrictions;

- Compulsory slaughter of all positive animals, under official supervision with sample collection forlaboratory diagnosis;


- Serological control of all remaining animals;

Infected Herd:

- All measures mencioned for positive herds;

- Desinfection of all premises, equipment and materials.


Notification system in placeBrucelosis is a notifiable disease.

193Portugal - 2013


Status as officially free of caprine brucellosis during the reporting yearFree regions

Região Autónoma dos Açores is officially free of ovine and caprine brucellosis, according to ComissionDecision 2003/44/CE of the 17th January 2003.


Serology is performed in sheep and goats older than 6 months of age.

The herds are classified and sampled according to Council Directive of 28 January 1991 on animal healthconditions governing intra-Community trade in ovine and caprine animals (91/68/EEC) and Decreto-Leinº244/2000 ( Sep. 27th ) for sheep and goats.

Frequency of the samplingThe herds are classified and sampled according to Council Directive of 28 January 1991 on animal healthconditions governing intra-Community trade in ovine and caprine animals (91/68/EEC) and Decreto-Leinº244/2000 ( Sep. 27th ) for sheep and goats


Diagnostic/analytical methods used


Serology:Sheep and goatsRose Bengal Test (RBT);Complement Fixation Test (CFT).

Bacteriology - Samples from:- live animals (milk, vaginal mucus, semen, aborted foetus, placenta);- dead animals (liver, spleen and lymph nodes)are plated in Farrel medium (Difco Tryptose Agar + SR209 Oxoid supplement + 5% horse serum)

Biochemical reactions - urease, catalase and oxidase.

Typing of isolates:- CO2 requirement;- H2S production;- Agglutination with monospecific antisera ( anti-A, anti-M and anti-R);- Growth on dyes: 1/50.000 and 1/100.000 of basic fucsin 1/50.000 and 1/100.000 of thionin.- Lysis by phages;- Differentiation of vaccine and field strains.

Vaccination policy

B. Brucella melitensis in goats

194Portugal - 2013


Vaccination of young animals with ReV1 is performed in some of the mainland regions: Norte, Centro,Alentejo and Algarve.

Other preventive measures than vaccination in placePre-movement tests are mandatory for breeding animals for the replacement in depopulated herds.


An Eradication Plan for sheep and goats, is carried out and supervised by DGAV.

Measures in case of the positive findings or single casesSuspected Herd:

- Herd under oficial surveillance;

- Epidemiological questionaire;


- Isolation of suspected animals in the herd;

- Sample collection for laboratory diagnosis.

Positive Herd:

- Herd under official restrictions;

- Compulsory slaughter of all positive animals, under official supervision with sample collection forlaboratory diagnosis;


- Serological control of all remaining animals;

Infected Herd:

- All measures mencioned for positive herds;

- Desinfection of all premises, equipment and materials.


Notification system in placeBrucelosis is a notifiable disease.

195Portugal - 2013


Status as officially free of ovine brucellosis during the reporting yearFree regions

See Brucella melitensis in goats.




Diagnostic/analytical methods usedSee Brucella melitensis in goats.

Vaccination policySee Brucella melitensis in goats.



Measures in case of the positive findings or single casesSee Brucella melitensis in goats.

C. Brucella melitensis in sheep

197Portugal - 2013

198


Portugal - 2013

Table Bovine brucellosis - data on herds - Community co-financed eradication programmes

Comments:1) 0



programme




Number ofherds

depopulated


Indicators

% herdcoverage



41098 30613 30254 63 51 2 3.17 98.83 .21 .17Continente

7047 5698 2863 25 21 0 0 50.25 .87 .73Região Autónoma dosAçores

48145 36311 33117 88 72 2 2.27 91.2 .27 .22Total :1)


199


Portugal - 2013

Table Ovine or Caprine brucellosis - data on herds - Community co-financed eradication programmes

Comments:1) 0



programme




Number ofherds

depopulated


Indicators

% herdcoverage



62497 62497 61055 672 491 13 1.93 97.69 1.1 .8Continente

62497 62497 61055 672 491 13 1.93 97.69 1.1 .8Total :1)


200


Portugal - 2013

Table Bovine brucellosis - data on animals - Community co-financed eradication programmes

Comments:1) 0






individually


Indicators



prevalenceRegion

Slaughtering



culled


slaughtered

1254670 969524 760860 681652 290 278 402 78.48 .04Continente

211122 133264 109237 90749 38 41 45 81.97 .03Região Autónoma dosAçores

1465792 1102788 870097 772401 328 319 447 78.9 .04Total :1)


201


Portugal - 2013

Table Ovine or Caprine brucellosis - data on animals - Community co-financed eradication programmes

Comments:1) 0






individually


Indicators



prevalenceRegion

Slaughtering



culled


slaughtered

2227202 2227202 2088376 1522259 3540 2998 4431 93.77 .17Continente

2227202 2227202 2088376 1522259 3540 2998 4431 93.77 .17Total :1)


202


Portugal - 2013

Table Bovine brucellosis - data on status of herds at the end of the period - Community co-financed eradication programmes

Comments:1) 0



Animals HerdsRegion


programme





10 566 58 4805 176 5592 1950 35122 28419 923439Continente

12 839 67 5246 188 6642 7625 166622 28419 923439Portugal

2 273 9 441 12 1050 5675 131500 0 0Região Autónoma dosAçores

0 0 0 0 24 1678 134 10492 376 13284 15250 333244 56838 1846878Total :1)


203


Portugal - 2013

Table Ovine or Caprine Brucellosis in countries and regions that do not receive Community co-financing for eradication programme

Comments:1) 0

Animals Number ofherds % Number of

herds

Number ofanimalstested

Number ofinfected herds

Region

% Number ofherds tested

Number ofanimals

tested withserologicalblood tests

Number ofanimalspositivemicrobiologically

Number ofsuspended

herds

Number ofanimalspositive

serologically

Number ofanimals

examinedmicrobiologically

Herds

Officially free herds Infected herds Investigations of suspect casesSurveillanceTotal number of existing

1042Portugal

1046 12692 1042 99.62 0 0 294 3498 0 198 10 10 0 4Região Autónoma dosAçores

1046 12692 2084 199.24 0 0 294 3498 0 198 10 10 0 4Total :1)


204


Portugal - 2013

Table Bovine brucellosis in countries and regions that do not receive Community co-financing for eradication programme

Comments:1) 0

Animals Number ofherds % Number of

herds

Number ofanimalstested

Number ofinfectedherds

Region

%

Number ofbovineherdstested

Number ofbovineherdstested

Number ofnotified

abortionswhatever

cause

Number ofisolationsof Brucellainfection

Number ofanimals or

poolstested

Number ofinfectedherds

Herds

Examination of bulk milk Information about Epidemiological investigationSerological tests

Total number ofexisting bovine

Number ofabortions

due toBrucellaabortus

Number ofanimals

tested withserologicalblood tests

Number ofsuspended

herds

Number ofanimals

examinedmicrobiologically

Number ofanimalspositivemicrobiologically

Serologically BST

Officially free herds Infected herdsInvestigations of suspect casesSurveillance

Number of positiveanimals

335 11093 335 100 0 0 41 925 0 0 0 0 0 0 0 0 0 0 0 0 0Algarve

2723Portugal

2388 60914 2388 100 0 0 804 9688 0 130 1887 0 4 0 0 0 0 0 0 0 0Região Autónoma dosAçores

2723 72007 5446 200 0 0 845 10613 0 130 1887 0 4 0 0 0 0 0 0 0 0Total :1)


205


Portugal - 2013

Table Ovine or Caprine brucellosis - data on status of herds at the end of the period - Community co-financed eradication programmes

Comments:1) 0



Animals HerdsRegion


programme





176 22562 268 26690 2121 47101 6526 435611 53406 1695238Continente

176 22562 268 26690 2121 47101 6526 435611 53406 1695238Portugal

0 0 0 0 352 45124 536 53380 4242 94202 13052 871222 106812 3390476Total :1)



2.7 YERSINIOSIS



Bacteriology: Internal method.- Samples are plated on Yersinia CIN Agar, or Yersinia Selective Agar (Oxoid) supplemented with YersiniaSelective supplement (Oxoid).- Biochemical reactions by API 20E strips or 32E.

A. Yersinia enterocolitica general evaluation

206Portugal - 2013

207


Portugal - 2013

2.7.2 Yersiniosis in humans

Table Yersinia in humans - Age distribution

1 115 to 24 years

2 1 125 to 44 years

2 245 to 64 years

1 165 years and older

6 4 2 0 0 0Total :

Y. enterocolitica Yersinia spp., unspecifiedAge distribution

All M F All M F

208


Portugal - 2013

Table Yersinia in humans - Seasonal distribution

1April

0May

1June

3July

1November

6 0Total :

Y.enterocoliti

ca

Yersiniaspp.,

unspecified


Months Cases Cases


2.8 TRICHINELLOSIS


2.8.2 Trichinellosis in humans

Reporting system in place for the human casesAbsente


Diagnostic/analytical methods usedELISA , Imunoblot and PCR

Notification system in place It is Notifiable disease


A. Trichinellosis in humans

209Portugal - 2013

210


Portugal - 2013

Table Trichinella in humans - Species/serotype distribution

0 0 0 0 0 0Trichinella spp., unspecified


Autochthon Inc.

Importedcases

ImportedInc.


Trichinella 0 0 0 0 0 0

211


Portugal - 2013

Table Trichinella in humans - Age distribution

0 0 0<1 year

0 0 01 to 4 years

0 0 05 to 14 years

0 0 015 to 24 years

0 0 025 to 44 years

0 0 045 to 64 years


0 0 0Age unknown

0 0 0Total :

Trichinella spp., unspecifiedAge distribution

All M F


2.8.3 Trichinella in animals

Monitoring systemFrequency of the sampling

All slaughtered animals.

Type of specimen takenTongue, masseter and diaphragm.

Case definitionDetection of one larvae of Trichinella.

Diagnostic/analytical methods usedMechanical digestion of pooled samples with magnetic stirrer (Comission Regulation (EC) N.º 2075/2005).

A. Trichinella in horses

212Portugal - 2013


Monitoring systemFrequency of the sampling

GeneralAll slaughtered animals.

Type of specimen takenGeneral

Pigs: diaphragm pillarsWild boars: tongue, diaphragm pillars, masseter

Methods of sampling (description of sampling techniques)General

As determined in Comission Regulation (EC) N.º 2075/2005 of 5 December.

Case definitionGeneral

Detection of one larvae of Trichinella.

Diagnostic/analytical methods usedGeneral

Mechanical digestion of pooled samples with magnetic stirrer (Comission Regulation (EC) N.º 2075/2005).

Notification system in placeNotifiable since 1953 by national law (Decreto-Lei n.º 39209, de 14 de Maio).

Results of the investigation including description of the positive cases and the verification ofthe Trichinella species

All results negative.

Fattening pigs raised under controlled housing conditions in integrated production systemAll results negative.

Fattening pigs not raised under controlled housing conditions in integrated production system

All results negative.

Breeding sows and boarsAll results negative.

National evaluation of the recent situation, the trends and sources of infectionCases of trichinelosis are not reported since < 1960.

Additional informationSpecial training in Trichinella detection on slaughterhouses and game activities is given to the meatinspection team.

B. Trichinella in pigs

213Portugal - 2013

214


Portugal - 2013

Table Trichinella in animals

RAM Census Officialsampling

animalsample >

organ/tissueDomestic Animal 1315 0

Pigs - fattening pigs - raised under controlledhousing conditions - Slaughterhouse - Surveillance

DGAV Census Officialsampling

animalsample >


Pigs - fattening pigs - not raised under controlledhousing conditions - Slaughterhouse - Surveillance


animalsample >


Pigs - breeding animals - raised under controlledhousing conditions - sows and boars -Slaughterhouse - Surveillance


animalsample >


Pigs - breeding animals - not raised under controlledhousing conditions - sows and boars -Slaughterhouse - Surveillance


animalsample >

organ/tissueDomestic Animal 3100 0Solipeds, domestic - horses - Slaughterhouse -

Surveillance


animalsample >


Pigs - breeding animals - raised under controlledhousing conditions - sows and boars -Slaughterhouse - Surveillance


animalsample >

organ/tissueDomestic Animal 396 0Pigs - breeding animals - unspecified - sows and

boars - Slaughterhouse - Surveillance


animalsample >

organ/tissueIntra EU trade Animal 107 0Pigs - breeding animals - unspecified - sows and

boars - Slaughterhouse - Surveillance


animalsample >


Pigs - fattening pigs - raised under controlledhousing conditions - Slaughterhouse - Surveillance


animalsample >

organ/tissueIntra EU trade Animal 1103871 0Pigs - fattening pigs - unspecified - Slaughterhouse -

Surveillance



Total unitspositive forTrichinella

T. spiralisTrichinella

spp.,unspecified

215


Portugal - 2013

Table Trichinella in animals


animalsample >

organ/tissueDomestic Animal 17929 0Pigs - fattening pigs - unspecified - Slaughterhouse -

Surveillance


animalsample >

organ/tissueDomestic Animal 6 0Pigs - fattening pigs - unspecified - Slaughterhouse -

Surveillance

RAA - LRV Census Officialsampling

animalsample >

organ/tissueDomestic Animal 26511 0Pigs - unspecified - Slaughterhouse - Surveillance


animalsample >

organ/tissueIntra EU trade Animal 10 0Solipeds, domestic - horses - Slaughterhouse -

Surveillance


animalsample >

organ/tissueDomestic Animal 14 0Wild boars - wild - Game handling establishment -

Surveillance

INIAV Objectivesampling

Officialsampling

animalsample >

organ/tissueDomestic Animal 29 0Wild boars - wild - Hunting - Surveillance



Total unitspositive forTrichinella

T. spiralisTrichinella

spp.,unspecified


2.9 ECHINOCOCCOSIS


Additional informationÂ§Diagnostic techniques:Direct examination test.

Â§On 1996 a program supervised by DGV was implemented in Alentejo (DRAAAL) (approved by Decision96/67/CE ). On 1998, besides Alentejo the same program was extended to Beira Interior (DRA BI).The program was extended, in 2000, to the Algarve (DRAALG).

This program consisted on:-deworming of all dogs present at rabies vaccination , by injection, performed by MunicipalityVeterinarians.-deworming tablets were given for a further deworming, in 2-3 weeks time.-deworming of dogs not present at rabies vaccination, but belonging to farms where sheep and goats withhidatidosis lesions were observed (the information of lesions in farm animals comes through the abattoir).-educational actions have been taken place, close to people (dog owners and farmers).

The program is still in place in the 3 referred regions.

A. Echinococcus spp. general evaluation

216Portugal - 2013


2.9.2 Echinococcosis in humans



Diagnostic/analytical methods usedELISA, Immunoblot and PCR.

Notification system in placeIt is a notifiable disease.


A. Echinococcus spp. in humans

217Portugal - 2013

218


Portugal - 2013

Table Echinococcus in humans - Species/serotype distribution

9 0 9 0 0 0E. granulosus

0 0 0 0 0 0E. multilocularis

0 0 0 0 0 0Echinococcus spp., unspecified


Autochthon Inc.

Importedcases

ImportedInc.


Echinococcus 9 0 9 0 0 0

219


Portugal - 2013

Table Echinococcus in humans - Age distribution

0 0 0 0 0 0 0 0 0<1 year

0 0 0 0 0 0 0 0 01 to 4 years

0 0 0 0 0 0 0 0 05 to 14 years

1 1 0 0 0 0 0 0 015 to 24 years

4 2 2 0 0 0 0 0 025 to 44 years

2 1 1 0 0 0 0 0 045 to 64 years

2 1 1 0 0 0 0 0 065 years and older

0 0 0 0 0 0 0 0 0Age unknown

9 5 4 0 0 0 0 0 0Total :

E. granulosus E. multilocularis Echinococcus spp., unspecifiedAge distribution



2.10 TOXOPLASMOSIS



- Direct examination test.- Serology - direct aglutination.- PCR.

A. Toxoplasmosis general evaluation

220Portugal - 2013


2.10.2 Toxoplasmosis in humans



Diagnostic/analytical methods used ISAGA , real time PCR and mice inoculation.

Notification system in placeOnly congenital toxoplasmosis is a notifiable disease.


A. Toxoplasmosis in humans

221Portugal - 2013

222


Portugal - 2013

Table Toxoplasma in humans - Species/serotype distribution

535Toxoplasma spp., unspecified

0 0Congenital cases

Cases Cases Inc.Species/serotype Distribution

Toxoplasma 535 0

223


Portugal - 2013

Table Toxoplasma in humans - Age distribution

152 86 67<1 year

11 2 91 to 4 years

5 4 15 to 14 years

54 4 5215 to 24 years

287 7 28025 to 44 years

17 8 945 to 64 years


4 0 4Age unknown

535 113 425Total :

Toxoplasma spp., unspecifiedAge distribution

All M F


2.11 RABIES


History of the disease and/or infection in the countryPortugal is free from Rabies since 1961.

In August 1984, the national authorities detected a case of rabies in a 2 months old puppy that came fromMaputo (Mozambique) and entered illegally in Portugal the10th August 1984. The animal was isolated andeuthanized. The disease was confirmed by direct immunofluorescence the 31st August of 1984. Theveterinary authorities maintained the implemented sanitary and prophylactic measures and since then, nofurther cases were detected and Portugal maintained its rabies free status.

National evaluation of the recent situation, the trends and sources of infectionPortugal is free from Rabies since 1961.

The vaccination of dogs is compulsory.

Rabies is a notifiable disease in animals and in humans.Additional information

National law (Decreto-Lei nº 314/2003, December the 17th and Portaria nº 81/2002, January the 24th)allows the dog rabies vaccination to be declared compulsory each year.

Vaccination is performed by the Municipality Veterinarians and by the small animal practitioners in theirprivate clinics.

Surveillance is based on the investigation of any clinical suspicion.Any cat or dog that bites, a human or another animal, is considered under suspicion and therefore keptunder veterinary surveillance in order to discard any case of rabies.

A. Rabies general evaluation

224Portugal - 2013


2.11.2 Lyssavirus (rabies) in animals

Monitoring systemCase definition

Laboratorial confirmation (positive result at the direct immunofluorescence test).

Vaccination policyBy national law (Decreto-Lei nº 314/2003 of December the 17th and Portaria nº 81/2002 of January the24th), the annual dog rabies vaccination for animals older than 3 months is compulsory.

Other preventive measures than vaccination in placeThe other preventive measures are included in the National Control programme.


The control Program is defined in the national law (Decreto Lei nº 314/2003, December the 17th) andconsists in Vaccination and Surveillance Measures for epidemiological survey with definition of specificrules for owners, for commercial purposes, for exhibits and for animal entrance in the country.

Measures in case of the positive findings or single casesThe measures are defined in the national and EU legislation.

Notification system in placeRage is a national notifiable disease since 1953.

National evaluation of the recent situation, the trends and sources of infectionPortugal is free from Rabies since 1961.

Additional informationIn Portugal the annual rabies vaccination is compulsory since 1925.

A. Rabies in dogs

225Portugal - 2013

226


Portugal - 2013

Table Rabies in animals

DGAV Suspectsampling

Officialsampling

animalsample >

brainDomestic Animal 1 1Cats - stray cats - Veterinary clinics - Clinical

investigations

DGAV Suspectsampling

Officialsampling

animalsample >

brainDomestic Animal 2 2Dogs - stray dogs - Veterinary clinics - Clinical

investigations


Samplingstrategy Sampler Sample type Sample origin Sampling unit Region Units tested

Total unitspositive forLyssavirus

(rabies)

Rabies virus(RABV) EBLV-1

1Cats - stray cats - Veterinary clinics - Clinicalinvestigations

2Dogs - stray dogs - Veterinary clinics - Clinicalinvestigations

EBLV-2Lyssavirus

(unspecifiedvirus)


2.12 STAPHYLOCOCCUS INFECTION


2.13 Q-FEVER


History of the disease and/or infection in the country*

National evaluation of the recent situation, the trends and sources of infection*

Relevance of the findings in animals, feedingstuffs and foodstuffs to human cases (as asource of infection)

*

Recent actions taken to control the zoonoses*

Suggestions to the European Union for the actions to be taken*

Additional information*

A. Coxiella general evaluation

227Portugal - 2013

228

2.14 WEST NILE VIRUS INFECTIONS



3. INFORMATION ON SPECIFIC INDICATORS OF ANTIMICROBIALRESISTANCE

229Portugal - 2013

230


Portugal - 2013

3.1 ESCHERICHIA COLI, NON-PATHOGENIC


3.1.2 Escherichia coli, non-pathogenic in foodstuffs

Table Escherichia coli, non-pathogenic in Food


Officialsampling food sample Domestic Single 100g 103 6 6Live bivalve molluscs - Processing plant -

Surveillance



weight Units tested

Total unitspositive forEscherichia

coli, non-pathogenic

E.coli, non-pathogenic,unspecified

231


Portugal - 2013

3.1.3 Antimicrobial resistance in Escherichia coli, non-pathogenic

Table Cut-off values used for antimicrobial susceptibility testing of Escherichia coli, non-pathogenic in Animals


NCCLS/CLSIEUCAST

EFSA 2Gentamicin

EFSA 16

Aminoglycosides

Streptomycin


EFSA 0.25Cefotaxime

EFSA 0.5

Cephalosporins

Ceftazidime




EFSA 256Sulfonamides

EFSA 64

Sulfonamides

Sulfamethoxazole




Test Method Used

Agar dilution

232


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of Escherichia coli, non-pathogenic in Animals




233


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of Escherichia coli, non-pathogenic in Feed


2Gentamicin

16

Aminoglycosides

Streptomycin


0.25Cefotaxime

0.5

Cephalosporins

Ceftazidime




256Sulfonamides

64

Sulfonamides

Sulfamethoxazole


2Trimethoprim Trimethoprim



Test Method Used

234


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of Escherichia coli, non-pathogenic in Feed

235


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of Escherichia coli, non-pathogenic in Food


2Gentamicin

16

Aminoglycosides

Streptomycin


0.25Cefotaxime

0.5

Cephalosporins

Ceftazidime




256Sulfonamides

64

Sulfonamides

Sulfamethoxazole


2Trimethoprim Trimethoprim



Test Method Used

236


Portugal - 2013

Table Cut-off values used for antimicrobial susceptibility testing of Escherichia coli, non-pathogenic in Food

237


Portugal - 2013

3.2 ENTEROCOCCUS, NON-PATHOGENIC


3.2.2 Antimicrobial resistance in Enterococcus, non-pathogenic isolates

Table Cut-off values for antibiotic resistance of E. faecalis in Animals


32Gentamicin

512

Aminoglycosides

Streptomycin



4Glycopeptides (Cyclicpeptides, Polypeptides) Vancomycin


4Oxazolidines Linezolid




Test Method Used

238


Portugal - 2013

Table Cut-off values for antibiotic resistance of E. faecalis in Animals




239


Portugal - 2013

Table Cut-off values for antibiotic resistance of E. faecalis in Feed


32Gentamicin

512

Aminoglycosides

Streptomycin










Test Method Used

240


Portugal - 2013

Table Cut-off values for antibiotic resistance of E. faecalis in Food


32Gentamicin

512

Aminoglycosides

Streptomycin










Test Method Used

241


Portugal - 2013

Table Cut-off values for antibiotic resistance of E. faecium in Animals


32Gentamicin

128

Aminoglycosides

Streptomycin







1Streptogramins Quinupristin/Dalfopristin




Test Method Used

242


Portugal - 2013

Table Cut-off values for antibiotic resistance of E. faecium in Feed


32Gentamicin

128

Aminoglycosides

Streptomycin











Test Method Used

243


Portugal - 2013

Table Cut-off values for antibiotic resistance of E. faecium in Food


32Gentamicin

128

Aminoglycosides

Streptomycin











Test Method Used


4. INFORMATION ON SPECIFIC MICROBIOLOGICAL AGENTS

244Portugal - 2013

245


Portugal - 2013

4.1 CRONOBACTER


4.1.2 Cronobacter in foodstuffs

Table Cronobacter in food


HACCP andown checks

food sample> milk Domestic Single 10 Millilitre 18 0Infant formula - liquid - Hospital or medical care




weight Units testedTotal unitspositive for

Cronobacter

Cronobactersakazakii

Cronobacterspp,

unspecified

246


Portugal - 2013

4.2 HISTAMINE


4.2.2 Histamine in foodstuffs

Table Histamine in food


Officialsampling

food sample> meat Domestic Batch 10 Gram 17

Fish - Fishery products from fish species associatedwith a high amount of histidine - not enzymematurated - Processing plant - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 7Fish - raw - frozen - Retail - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 6Fishery products, unspecified - ready-to-eat -

Packing centre - Surveillance


Officialsampling food sample Domestic Batch 10 Gram 5Fishery products, unspecified - ready-to-eat -



Officialsampling food sample Domestic Batch 10 Gram 14Fishery products, unspecified - ready-to-eat - Retail -

Surveillance



weight Units testedTotal units in

non-conformity

<= 100 mg/kg>100 - <= 200mg/kg

247


Portugal - 2013

Table Histamine in food

0

Fish - Fishery products from fish species associatedwith a high amount of histidine - not enzymematurated - Processing plant - Surveillance

0Fish - raw - frozen - Retail - Surveillance

0Fishery products, unspecified - ready-to-eat -Packing centre - Surveillance

0Fishery products, unspecified - ready-to-eat -Processing plant - Surveillance

0Fishery products, unspecified - ready-to-eat - Retail -Surveillance

>200 - <= 400mg/kg > 400 mg/kg


4.3 STAPHYLOCOCCAL ENTEROTOXINS


4.3.2 Staphylococcal enterotoxins in foodstuffs

Additional informationAnalytical method:Milk and dairy products - VIDAS SET2 (European screening method of CRL, Milk and Milk Products,version 3, 2006 May).Other products - VIDAS SET2 (AOAC validation).

A. Staphylococcal enterotoxins in foodstuffs

248Portugal - 2013

249


Portugal - 2013

Table Staphylococcal enterotoxins in food


Officialsampling




Officialsampling




Officialsampling




Officialsampling




Officialsampling




Officialsampling


Dairy products (excluding cheeses) - milk powderand whey powder - Processing plant - Surveillance


Officialsampling




Officialsampling




Officialsampling





weight Units tested


Staphylococcal

enterotoxins

250


Portugal - 2013

Table Staphylococcal enterotoxins in food


Officialsampling




Officialsampling




Officialsampling





weight Units tested


Staphylococcal

enterotoxins


5. FOODBORNE

Foodborne outbreaks are incidences of two or more human cases of the same disease orinfection where the cases are linked or are probably linked to the same food source. Situation, inwhich the observed human cases exceed the expected number of cases and where a same foodsource is suspected, is also indicative of a foodborne outbreak.

251Portugal - 2013


System in place for identification, epidemological investigations and reporting of foodborneoutbreaks

*

Description of the types of outbreaks covered by the reporting:*

National evaluation of the reported outbreaks in the country:Trends in numbers of outbreaks and numbers of human cases involved

*

Relevance of the different causative agents, food categories and the agent/food categorycombinations

*

Relevance of the different type of places of food production and preparation in outbreaks*

Evaluation of the severity and clinical picture of the human cases*

Descriptions of single outbreaks of special interest*

Control measures or other actions taken to improve the situation*

Suggestions to the European Union for the actions to be taken*

Additional information*

A. Foodborne outbreaks

252Portugal - 2013


0 unknown unknown unknown 0 0Salmonella - S.Typhimurium

0 unknown unknown unknown 0 0Salmonella - S.Enteritidis

0 unknown unknown unknown 0 0Salmonella - Otherserovars

0 unknown unknown unknown 0 0Campylobacter

0 unknown unknown unknown 0 0Listeria - Listeriamonocytogenes

0 unknown unknown unknown 0 0Listeria - OtherListeria

0 unknown unknown unknown 0 0Yersinia

0 unknown unknown unknown 1 1Escherichia coli,pathogenic -Verotoxigenic E. coli(VTEC)

0 unknown unknown unknown 0 0Bacillus - B. cereus

0 unknown unknown unknown 0 0Bacillus - OtherBacillus

0 unknown unknown unknown 5 5Staphylococcalenterotoxins

0 unknown unknown unknown 1 1Clostridium - Cl.botulinum

0 unknown unknown unknown 1 1Clostridium - Cl.perfringens

Weak evidence or no vehicle outbreaks

Num

ber o

f out

brea

ks

Hum

an c

ases

Hos

pita

lized

Dea

ths

Stro

ng e

vide

nce

Num

ber o

fO

utbr

eaks

Tota

l num

ber o

f out

brea

ks

Table Foodborne Outbreaks: summarised data

253Portugal - 2013


0 unknown unknown unknown 0 0Clostridium - OtherClostridia

0 unknown unknown unknown 0 0Other Bacterial agents- Brucella

0 unknown unknown unknown 0 0Other Bacterial agents- Shigella

0 unknown unknown unknown 0 0Other Bacterial agents- Other Bacterialagents

0 unknown unknown unknown 0 0Parasites - Trichinella

0 unknown unknown unknown 0 0Parasites - Giardia

0 unknown unknown unknown 0 0Parasites -Cryptosporidium

0 unknown unknown unknown 0 0Parasites - Anisakis

0 unknown unknown unknown 0 0Parasites - OtherParasites

0 unknown unknown unknown 2 2Viruses - Norovirus

0 unknown unknown unknown 0 0Viruses - Hepatitisviruses

0 unknown unknown unknown 0 0Viruses - OtherViruses

0 unknown unknown unknown 0 0Other agents -Histamine

0 unknown unknown unknown 0 0Other agents - Marinebiotoxins

0 unknown unknown unknown 0 0Other agents - OtherAgents


Num

ber o

f out

brea

ks

Hum

an c

ases

Hos

pita

lized

Dea

ths

Stro

ng e

vide

nce

Num

ber o

fO

utbr

eaks

Tota

l num

ber o

f out

brea

ks

254Portugal - 2013


0 unknown unknown unknown 8 8Unknown agent


Num

ber o

f out

brea

ks

Hum

an c

ases

Hos

pita

lized

Dea

ths

Stro

ng e

vide

nce

Num

ber o

fO

utbr

eaks

Tota

l num

ber o

f out

brea

ks

255Portugal - 2013


C. botulinum

FBO Code

1Number of outbreaks

1Number of human cases

1Number of hospitalisations

0Number of deaths

Other foodsFood vehicle

Suspected food - Smoked sausage made with bread, poultry meat and garlic.More food vehicleinformation

Descriptive epidemiological evidenceNature of evidence

HouseholdOutbreak type

UnknownSetting

UnknownPlace of origin of problem

DomesticOrigin of food vehicle

UnknownContributory factorsMixed Outbreaks (OtherAgent)

Botulism neurotoxin B was detected in clinical samples (blood and stools), accordingto CDC Atlanta specific procedures.Additional information

Value

Table Foodborne Outbreaks: detailed data for ClostridiumPlease use CTRL for multiple selection fields

256Portugal - 2013


C. perfringens

FBO Code




0Number of deaths

Mixed foodFood vehicle

Octopus fricassee.More food vehicleinformation

Detection of causative agent in food vehicle or its component - Symptoms and onsetof illness pathognomonic to causative agentNature of evidence

GeneralOutbreak type

Canteen or workplace cateringSetting

Canteen or workplace cateringPlace of origin of problem


Storage time/temperature abuseContributory factorsC. perfringens 3.2E+07 cfu/g and Coagulase-positive staphylococci 1.8E+03 cfu/gproducer of Staphylococcal enterotoxin (unspecified)

Mixed Outbreaks (OtherAgent)

Also CF03A-Inadequate heat treatmentAdditional information

Value

257Portugal - 2013


Verotoxigenic E. coli (VTEC) - VTEC, unspecified

FBO Code




0Number of deaths

Herbs and spicesFood vehicle

Parsley (Vegetable fresh, intended to eaten raw)More food vehicleinformation



School or kindergartenSetting

School or kindergartenPlace of origin of problem


Unprocessed contaminated ingredientContributory factorsMixed Outbreaks (OtherAgent)

Presumptive detection of STEC (Positive sample for stx2)Additional information

Value

Table Foodborne Outbreaks: detailed data for Escherichia coli, pathogenicPlease use CTRL for multiple selection fields

258Portugal - 2013


Enterotoxin A

FBO Code



unknownNumber of hospitalisations

0Number of deaths

Bakery productsFood vehicle

Birthday cakeMore food vehicleinformation



Residential institution (nursing home or prison or boarding school)Setting

Restaurant or Cafe or Pub or Bar or Hotel or Catering servicePlace of origin of problem


Infected food handlerContributory factorsMixed Outbreaks (OtherAgent)

Also CF06A - Storage time/temperature abuse and CF01A - Cross-contaminationAdditional information

Value

Table Foodborne Outbreaks: detailed data for Staphylococcal enterotoxinsPlease use CTRL for multiple selection fields

259Portugal - 2013


Enterotoxin A

FBO Code


unknownNumber of human cases

unknownNumber of hospitalisations

0Number of deaths


Prawn rissolesMore food vehicleinformation



Camp or picnicSetting

HouseholdPlace of origin of problem


Storage time/temperature abuseContributory factors

Enterotoxins A+DMixed Outbreaks (OtherAgent)

Enterotoxins A+D (Also CF01A - Cross-contamination)Additional information

Value

260Portugal - 2013


Enterotoxin, unspecified

FBO Code




0Number of deaths

Bakery productsFood vehicle

CakeMore food vehicleinformation






Storage time/temperature abuseContributory factorsMixed Outbreaks (OtherAgent)

Also CF01A - Cross-contaminationAdditional information

Value

261Portugal - 2013


Enterotoxin A

FBO Code




0Number of deaths


Duck riceMore food vehicleinformation



HouseholdSetting



Storage time/temperature abuseContributory factorsMixed Outbreaks (OtherAgent)

Also CF01A - Cross-contaminationAdditional information

Value

262Portugal - 2013


Enterotoxin A

FBO Code




0Number of deaths


Rice with shredded chickenMore food vehicleinformation






Infected food handlerContributory factorsMixed Outbreaks (OtherAgent)

Also CF06A - Storage time/temperature abuse and CF01A - Cross-contaminationAdditional information

Value

263Portugal - 2013


Unknown

FBO Code




0Number of deaths


UnknownMore food vehicleinformation





UnknownOrigin of food vehicle

UnknownContributory factorsMixed Outbreaks (OtherAgent)Additional information

Value

Table Foodborne Outbreaks: detailed data for Unknown agentPlease use CTRL for multiple selection fields

264Portugal - 2013


Unknown

FBO Code




0Number of deaths






Residential institution (nursing home or prison or boarding school)Place of origin of problem



Value

265Portugal - 2013


Unknown

FBO Code




0Number of deaths









Value

266Portugal - 2013


Unknown

FBO Code




0Number of deaths





HouseholdSetting




Value

267Portugal - 2013


Unknown

FBO Code




0Number of deaths





HouseholdSetting

UnknownPlace of origin of problem



Value

268Portugal - 2013


Unknown

FBO Code




0Number of deaths









Value

269Portugal - 2013


Unknown

FBO Code




0Number of deaths









Value

270Portugal - 2013


Unknown

FBO Code




0Number of deaths





Restaurant or Cafe or Pub or Bar or Hotel or Catering serviceSetting




Value

271Portugal - 2013


Calicivirus - norovirus (Norwalk-like virus)

FBO Code




0Number of deaths









Norovirus GII was detected in clinical samples (stools).Additional information

Value

Table Foodborne Outbreaks: detailed data for VirusesPlease use CTRL for multiple selection fields

272Portugal - 2013


Calicivirus - norovirus (Norwalk-like virus)

FBO Code




0Number of deaths









Norovirus GII was detected in clinical samples (stools).Additional information

Value

273Portugal - 2013

portugal - european food safety authority · portugal - 2013 report on trends and sources of...

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