polysaccharides analysis purification of oligosaccharides
TRANSCRIPT
Glycosidic composition
Molecular weight and polydispersity of polysaccharides
Polysaccharides analysis Purification of oligosaccharides
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Separation of cellodextrins from DP 1 to 7 on a CarboPac PA1 column (Dionex)
Exclusion Size Chromatography (SEC) with Differential Refractometric Detector
Separation and purification of maltodextrins from DP 1 to 10 on a Biogel P2 column (BioRad)
High Performance Liquid Chromatography (HPLC) with Differential Refractometric Detector and UV Detector
Separation of oligochitins from DP 2 to 6 on a NH2 column
High Performance Anion Exchange Chromatography with Pulsed Amperometric Detector (HPAEC-PAD)
Separation of monosaccharides on a CarboPac PA1 column (Dionex)
High Performance Anion Exchange Chromatography with Pulsed Amperometric Detector (HPAEC-PAD)
Size Exclusion Chromatography (SEC) equipped with double detection: Differential Refractometer, Multi- Angles Light Scattering
Analysis of a mixture of pullulans on a OH-Pak SB-803HQ Shodex column
Gas Chromatography with Flam Ionisation Detector (FID)
Separation of methyl glycoside trimethylsilyl derivatives of monosaccharides on a HP-5MS column
Fuc
Rha
Ara
Gal Glc
Man Xyl Rib Gal A
Glc A Glc
DP2
DP3
DP4
DP7
DP6
DP5
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GlcNAc
GlcA
GlcA Glc
Glc
GalA Gal
Gal Gal
GalA
Man
Man
GlcA GalA
GlcA
GlcNAc
Myo
212 000 Da 22 800 Da
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Gas Chromatography with Mass Spectrometry Detector (GC/MS)
PMAA derivatives, separated on a Supelco 2380 column
Glycosidic linkages
PMAA derivatives Corresponding
substituted residue
1,3,5-tri-O-acetyl-1-deuterio-6 deoxy 2,4-
di-O-methyl D-rhamnitol
!3)-Rha-(1!
1,3,5-tri-O-acetyl-1-deuterio-2,4,6-tri-O-methyl D-mannitol
!3)-Man-(1!
1,5-di-O-acetyl-1-deuterio-6 deoxy
2,3,4-di-O-methyl D-rhamnitol
-Rha-(1!
1,4,5-tri-O-acetyl-1-deuterio-2,3,6-tri-O-
methyl D-glucitol !4)-Glc-(1!
DP2
DP1
DP3 DP4
DP5
DP 8,9,10
DP7 DP6
DP2
DP3
DP4
DP5
DP6