pöschl et al., suppl. material supplementary material: suppl. figure 1: related to figure 1....
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Pöschl et al., Suppl. Material
Supplementary Material:
Suppl. Figure 1: Related to Figure 1. Details on the 423 analyzed human
medulloblastoma samples.
Suppl. Figure 2: Related to Figure 1. The top 25 upregulated genes per human
medulloblastoma subgroup out of the differentially expressed genes used for comparative analyses of human and murine data.
Suppl. Figure 3: Related to Figure 1. Mouse Wnt Medulloblastomas model well
human WNT medulloblastomas, independent of TP53 mutation status.
Suppl. Figure 4: Related to Figure 2. Human SHH-medulloblastomas form a
„infant“ and “adult“ cluster, but no distinct “childhood“ cluster.
Suppl. Figure 5: Related to Figure 2. Validation of significantly differentially
expressed genes between infant and adult human SHH-medulloblastomas, that were used for analysis.
Suppl. Figure 6: Related to Figure 2.Heatmap showing 287 similarly expressed
genes of adult human SHH medulloblastoma and murine Shh medulloblastoma mouse
models
Suppl. Table 1: Related to Figure 1. Raw AGDEX values and Perm P values
shown in Figure 1A.
Suppl. Table 2: Related to Figure 2. Raw AGDEX values and Perm P values
shown in Figure 2A.
Suppl. Table 3: Related to Figure 2 and Supplementary Figure 6. List of the 287 similarly expressed genes of
adult human SHH medulloblastoma and murine Shh medulloblastoma mouse models.
0 100 200 3000
50
100WNTSHHGroup 3Group 4
Time [months]
Ove
rall
surv
ival
[%
]
Supplementary Figure 1: Epidemiological and clinical data of the 423 analyzed human medulloblastoma samples.
Pöschl et al., Suppl. Figure 1
Molecular subgroup n [% of all patients] 53 [12.53] 112 [26.48] 94 [22.22] 164 [38.77]
Agegroup n % of subgroup n % of subgroup n % of subgroup n % of subgroupInfant (≤ 3 years) 0 0 29 25.89 25 26.6 8 4.88
Child (> 3 < 16 years) 44 83.02 27 24.11 63 67.02 131 79.88
Adult (≥ 16 years) 9 16.98 54 48.21 3 3.19 22 13.41Not available 2 1.79 3 3.19 3 1.83
Gender n % of subgroup n % of subgroup n % of subgroup n % of subgroupFemale 28 52.83 53 47.32 28 29.79 48 29.27Male 25 47.17 59 52.68 66 70.21 116 70.73
M stage n % of subgroup n % of subgroup n % of subgroup n % of subgroupM - 40 75.47 77 68.75 57 60.64 86 52.44
M + 5 9.43 14 12.5 31 32.98 60 36.59Not available 8 15.09 21 18.75 6 6.38 18 10.98
Histology n % of subgroup n % of subgroup n % of subgroup n % of subgroupClassic 49 92.45 38 33.93 59 62.77 123 75
Desmoplastic 0 0 42 37.5 5 5.34 9 5.49Extensively nodular 0 0 3 2.68 0 0 0 0Large cell/ anaplastic 4 7.55 16 14.29 19 20.21 18 10.98
Medullomyoblastoma 0 0 0 0 3 3.2 0 0Not available 0 0 13 11.61 8 8.51 14 8.54
10 year survival rate
WNT SHH Group 3 Group 4
97.8% 61.8% 43.5% 67.7%
WNT SHH Group 3 Group 4
Supplementary Figure 2: Heatmap showing the top 25 upregulated genes per human medulloblastoma subgroup. High expression is shown in red, low expression is shown in blue.
Pöschl et al., Suppl. Figure 2
data not available
TP53
TP53 mutated (n=4)TP53 WT (n=30)
Histology
Classic (n=34)
Gender
Female (n=20)Male (n=14)
Age group
Child (n=27)Adult (n=7)
Status
Alive (n=31)Dead (n=2)
Supplementary Figure 3A: Non-hierarchical average linkage clustering (20% variance filter) of 34 human WNT tumors with known TP53 mutation status. The four TP53 mutated WNT tumors did not cluster together.
TP53Status
HistologyGender
Age group
Pöschl et al., Suppl. Figure 3
Age group:
Infant (≤ 3 years; n=29) Child (> 3 years < 16 years; n=27) Adult (≥ 16 years; n=54)
Supplementary Figure 4: Based on gene expression, SHH childhood medulloblastoma cases do not form a distinct cluster. Hierarchical clustering based on significantly differentially expressed genes using an “infant“, “childhood“ and “adult“ group distinguished an “infant“ and “adult“ cluster, but no distinct “childhood“ cluster. Instead childhood tumors clustered variably to the “infant“ or “adult“ SHH subgroup, with the majority been found in the „adult“ subgroup.
Pöschl et al., Suppl. Figure 4
Northcott dataset
200 (out of 332)
Current dataset
705101
Age group
Infant (n=29)
Adult (n=54)
-2 0 +2
Supplementary Figure 5: 705 genes were found to be significantly differentially expressed between infantile and adult human SHH medulloblastomas in the current dataset (out of 10333 orthologue genes present on all examined platforms). The Venn diagramm shows the overlap of significant genes with the Northcott study (Northcott et al. 2011a). The Northcott study identified 332 significant differentially expressed genes – of these 200 were also present in our current dataset. 101 genes were found to be differentially expressed in both studies. Expression of these 101 genes in our dataset is displayed in the heatmap.
Pöschl et al., Suppl. Figure 5
-2 0 +1.5
SHH infant (n=29)
SHH adult (n=54)
Mouse modelsn=64
Supplementary Figure 6: Heatmap showing 287 signifcant differentially expressed genes when comparing human infant SHH medulloblastoma to human adult medulloblastoma and murine medulloblastoma models. Details on the genes can be found in table S3.
Pöschl et al., Suppl. Figure 6
Genotype WNT PERM P SHH PERM P Group 3 PERM P Group 4 PERM P
Blbp-cre::Ctnnb1(ex3)Fl/+ Trp53Fl/Fl (n=6) 0.3009 0.0058 0.2339 0.0284 0.2275 0.0155 0.0569 0.5280
Ptch1+/- (n=12) 0.3134 0.0359 0.4535 0.0078 0.3524 0.0409 0.1771 0.2049
Math1-creERT2::Ptch1Fl/Fl (n=4) 0.2416 0.0743 0.3717 0.0175 0.2869 0.0360 0.1489 0.2433
Nestin-creERT2::Ptch1Fl/Fl (n=4) 0.2729 0.0377 0.4057 0.0092 0.3104 0.0085 0.1776 0.1119
Ptch1+/-Trp53-/- (n=3) 0.1759 0.0059 0.2101 0.0068 0.2019 0.0015 0.1068 0.0891
Ptch1+/-Cdkn2C -/- (n=8) 0.2680 0.0364 0.4083 0.0072 0.3248 0.0083 0.2261 0.0506
Tlx3-cre:: SmoM2Fl/+ (n=3) 0.1648 0.1195 0.3152 0.0100 0.2399 0.0364 0.2103 0.0678
Olig2-tva-cre:: SmoM2Fl/+ (n=7) 0.2506 0.0855 0.3763 0.0139 0.3001 0.0728 0.1740 0.2205
Math1-cre: :SmoM2Fl/+ (n=3) CB 0.1959 0.1026 0.3652 0.0175 0.2338 0.1199 0.1407 0.2863
Math1-cre: :SmoM2Fl/+ (n=3) CN 0.2314 0.0503 0.3858 0.0124 0.2594 0.0629 0.1595 0.1984
hGFAP-cre: :SmoM2Fl/+ (n=3) CB 0.1862 0.0826 0.3490 0.0115 0.2096 0.1648 0.1235 0.2963
hGFAP-cre: :SmoM2Fl/+ (n=3) CN 0.2396 0.0622 0.3946 0.0129 0.2623 0.0927 0.1432 0.2671
Nestin-cre::Trp53Fl/Fl Cdkn2C-/- (n=8) 0.3143 0.0783 0.4542 0.0121 0.3597 0.0927 0.1457 0.3341
Sufu+/-Trp53-/- (n=3) 0.2213 0.0275 0.2901 0.0067 0.2446 0.0253 0.0678 0.4087
MycDNTrp53 (Prom1+ Lin- stem cells) (n=5) 0.3514 0.0221 0.4557 0.0049 0.4025 0.0231 0.1632 0.2364
MycDNTrp53 (Prom1+ Lin+ progenitors) (n=5) 0.3546 0.0208 0.4582 0.0052 0.4142 0.0177 0.1652 0.2210
Cdkn2c-/-Trp53-/-, mMyc (n=14) 0.3580 0.0538 0.4711 0.0227 0.4022 0.1241 0.0963 0.5570
Cdkn2c-/-Trp53-/-,hMyc (n=3) 0.3621 0.0369 0.4638 0.0159 0.4149 0.0835 0.1156 0.4567
Cdkn2c-/-Trp53-/-, MycN (n=11) 0.3731 0.0452 0.4965 0.0148 0.4031 0.1017 0.0983 0.5329
Glt1-tTA::TRE-MYCN/Luc (n=32) 0.3568 0.0071 0.3531 0.0012 0.4361 0.0002 0.2554 0.0172
Supplementary Table 1: Raw AGDEX values and Perm P values when comparing different murine medulloblastoma models to the four subgroups of medulloblastoma. The best match for each model is highlighted in orange.
Pöschl et al., Suppl. Table 1
GenotypeTumor
localisation INFANT PERM P ADULT PERM P
Ptch1+/- (n=12) CB 0.1985 0.4509 0.3591 0.0156
Math1-creERT2::Ptch1Fl/Fl (n=4) CB 0.2280 0.4308 0.3066 0.0371
Nestin-creERT2::Ptch1Fl/Fl (n=4) CB 0.2378 0.3719 0.2929 0.0379
Ptch1+/-Trp53-/- (n=3) CB 0.1511 0.4311 0.3427 0.0057
Ptch1+/-Cdkn2C -/- (n=8) CB 0.1776 0.5288 0.2752 0.0775
Tlx3-cre:: SmoM2Fl/+ (n=3) CB 0.1972 0.3754 0.1968 0.0559
Olig2-tva-cre:: SmoM2Fl/+ (n=7) CB 0.1607 0.4976 0.3242 0.0150
Math1-cre: :SmoM2Fl/+ (n=3) CB 0.1461 0.5995 0.3049 0.0215
Math1-cre: :SmoM2Fl/+ (n=3) CN 0.1424 0.6068 0.3511 0.0148
hGFAP-cre: :SmoM2Fl/+ (n=3) CB 0.1151 0.6557 0.2777 0.0343
hGFAP-cre: :SmoM2Fl/+ (n=3) CN 0.1370 0.6111 0.3176 0.0234
Nestin-cre::Trp53Fl/Fl Cdkn2C-/- (n=8) CB 0.2148 0.4406 0.3474 0.0224
Sufu+/-Trp53-/- (n=3) CB 0.2144 0.3244 0.3398 0.0101
Supplementary Table 2: Raw AGDEX values and Perm P values when comparing different murine Shh medulloblastoma models to infantile or adult human SHH medulloblastoma cases. Best matches of each model are highlighted in orange.
Pöschl et al., Suppl. Table 2
Pöschl et al., Suppl. Table 3High in human infant SHH Tumors
MEAN ADULT and MOUSE
TumorsSD Adult and
Mouse TumorsMean Infant
TumorsSD Infant Tumors
adjusted p-value FDR
ACTA1 -0,2736967 0,40240562 0,0915157 0,23518874 8,84E-06 1,31E-07ADD3 -0,3986583 0,68905705 0,840792 1,3394148 0,01567845 1,01E-04
AMPD3 -0,5510598 0,4342057 0,2113985 0,7382354 0,00359565 2,55E-05AMPH -0,4067815 0,58926404 0,5825517 1,1056875 0,0231441 1,45E-04ANK3 -0,2285457 0,7543664 1,8847224 1,2302096 1,98E-07 4,17E-09
APBB1 -0,5611082 0,43113378 0,6217667 1,1803654 0,0050552 3,44E-05APLP2 -0,6795695 0,48893243 0,3736917 0,8985004 4,46E-04 3,91E-06
ARHGAP44 -0,711981 0,29055968 -0,026718 0,79870623 0,03665173 2,22E-04ARNTL -0,810629 0,41836005 -0,2718194 0,56255317 0,01189624 7,81E-05
ASPHD2 -0,1134203 0,7686489 0,7413253 0,9500732 0,02797623 1,73E-04ATP5B -0,4874611 0,6474947 0,7393577 0,880387 1,97E-05 2,63E-07
ATP6V1G2 -0,5616174 0,34636083 0,8560063 1,4997127 0,01088189 7,18E-05BAIAP2 -0,5723038 0,3337072 0,03755 0,664901 0,01719819 1,10E-04
C14ORF169 -0,6777008 0,9619623 0,3167307 0,57554734 3,69E-07 7,49E-09C15ORF38-
AP3S2,AP3S2-0,8657904 0,5819554 -0,2318643 0,6908973 0,02257109 1,42E-04
C1QTNF4 -0,732315 0,25896174 -0,4156726 0,34892857 0,02396127 1,50E-04C21ORF59 -0,3458999 0,73729384 0,9020283 1,0727334 5,69E-04 4,90E-06
C4ORF32 -0,6650406 0,4224659 0,1392156 0,73967427 0,00160183 1,23E-05CADPS2 -0,4884835 0,48272508 1,0444686 1,4119722 0,00155643 1,20E-05CAMK4 -0,3186014 0,4471958 0,667687 0,9503742 0,00296404 2,13E-05CASQ2 -0,4050122 0,21455474 -0,1473773 0,14752753 1,34E-07 2,86E-09CBLN1 -0,3715423 0,282928 1,776182 1,6063838 4,85E-05 5,59E-07
CCDC106 -0,6514843 0,7035532 0,1099939 0,8432816 0,02731438 1,70E-04CCDC92 -0,6008607 0,6214308 0,7013466 1,450703 0,02189356 1,38E-04
CD200 -0,5056658 0,53921175 -0,0267494 0,5454667 0,04691149 2,80E-04CEP85 -0,538823 0,68906033 0,2920238 0,96610135 0,0428549 2,57E-04
CKMT1B,CKMT1A -0,6224688 0,34742752 0,1377923 0,8769979 0,03164208 1,93E-04CLSTN3 -0,5952044 0,3632332 0,0504066 0,7082706 0,01892104 1,20E-04
CORO1A -0,5336767 0,4464921 0,7978107 1,3243732 0,00469368 3,23E-05CPEB2 -0,7194768 0,45191312 0,1469895 0,65161407 5,07E-05 5,80E-07CPNE8 -0,4476648 0,58020645 0,6779916 0,89813226 1,55E-04 1,54E-06CYFIP2 -0,917551 0,3901158 -0,043476 0,73000425 3,03E-04 2,76E-06
ECHDC2 -0,4826384 0,66599435 1,0551793 1,2127678 1,14E-04 1,21E-06EFNB2 -0,3458508 0,8008046 1,0487882 1,1249865 1,77E-04 1,74E-06EMCN -0,424006 0,665607 1,2962767 1,6768299 0,00347934 2,49E-05
EPHA7 -0,4174543 0,44034973 -0,2080296 0,09831297 0,00285097 2,08E-05FAM19A5 -0,4528866 0,73674846 2,0459554 1,2566425 7,53E-09 3,36E-10
FAM32A -0,0111569 0,95822155 0,816252 0,8409857 0,01390718 9,05E-05FCGRT -0,0725804 0,77054715 1,512644 1,5657283 0,00440052 3,06E-05
FEZ1 -0,4137424 0,5256489 1,58211 1,4469427 2,80E-05 3,52E-07FGF19 -0,294138 0,61156964 1,206404 1,6895546 0,02457635 1,53E-04FOLR2 0,251809 0,8689384 1,9020073 1,6196111 0,00399639 2,82E-05FRMD6 -0,317896 0,5213716 0,657405 0,73091483 4,38E-05 5,17E-07
GALNT12 -0,3493126 0,4837105 0,301852 0,5663032 7,73E-04 6,47E-06GAS6 -0,6129618 0,42372066 0,0895829 0,46331364 2,87E-06 4,78E-08
GFPT2 -0,4169517 0,5699038 0,1298797 0,60458165 0,03042219 1,87E-04GFRA2 -0,3594359 0,44081858 0,6050958 1,1089619 0,03051798 1,87E-04GNA14 -0,269141 0,64814085 0,9480154 1,2687906 0,00967899 6,41E-05
GOT1 -0,6666146 0,37000605 0,7237064 1,5585674 0,02256819 1,42E-04GPHN -0,5273474 0,6368207 0,314085 0,6827042 2,41E-04 2,26E-06
GPI -0,7036107 0,54594487 0,3179387 1,0198526 0,00504837 3,45E-05HECW1 -0,5893436 0,29053724 0,398977 0,8163084 2,79E-04 2,60E-06HMOX2 -0,7327574 0,4980786 0,275969 1,0215478 0,00618303 4,16E-05JARID2 0,091192 0,83122766 0,955925 0,7926702 0,0023733 1,75E-05
KAZN -0,4838162 0,40413776 0,0188276 0,5786221 0,03899058 2,36E-04KCNJ3 -0,5313013 0,40783623 0,646928 1,0717127 0,00120552 9,60E-06KCNK1 -0,3665393 0,43104228 0,297203 0,6800656 0,00820862 5,48E-05
KIAA1324L 0,0676648 0,9981148 1,1758844 1,0374626 0,00296822 2,14E-05KIF1B -0,7764533 0,4827391 -0,1885803 0,549708 0,00255587 1,87E-05KIF3C -0,664698 0,4528029 0,5800008 1,2612157 0,00619578 4,15E-05KLC1 -0,5468903 0,5793387 0,748115 1,0853808 3,21E-04 2,87E-06LHX8 -0,2660545 0,68339926 1,2022597 1,356885 0,00167428 1,28E-05
LIMK2 -0,5943137 0,6228112 0,9412426 1,0448354 6,02E-06 9,40E-08LNX1 -0,465056 0,5787848 0,4318597 0,6621536 3,35E-05 4,18E-07
LPHN1 -0,5410462 0,39656642 -0,2379625 0,24256623 9,17E-04 7,53E-06LURAP1L -0,3093317 0,616717 1,2649185 0,83099794 1,71E-08 6,11E-10
LYPLA2 -0,4838305 0,7399838 0,1213743 0,5470517 0,00352665 2,52E-05MAPK8IP2 -0,5336943 0,49410248 0,0362324 0,50918424 0,00142994 1,13E-05
MAPK9 -0,329942 0,63446486 1,1104557 1,0063814 1,15E-05 1,63E-07MCTP1 -0,466345 0,4392676 0,5082528 1,0660535 0,01705517 1,09E-04
MPG -0,23558 1,1221805 1,8027818 1,4923702 2,70E-05 3,43E-07MREG -0,4121273 0,40530333 0,9126195 0,7527454 1,19E-07 2,70E-09MSRA -0,3251927 0,6705541 1,964723 1,1694646 1,05E-08 4,52E-10
MTFP1 -0,7826174 0,4498654 0,019879 0,64602846 1,83E-04 1,78E-06MTUS2 -0,5276096 0,389134 0,280437 0,75469774 0,00200777 1,50E-05
NAB2 -0,3481763 0,76936394 0,5228838 0,8567274 0,0058251 3,93E-05NCDN -0,6260417 0,29826325 -0,2350343 0,45083514 0,03723336 2,26E-04NEFL -0,5429206 0,28257912 -0,079837 0,269144 1,24E-07 2,77E-09NEFM -0,601053 0,24664934 -0,101582 0,33119467 3,81E-06 6,17E-08
NEUROD1 -0,3897462 0,8010917 1,239071 0,6385459 3,01E-13 5,02E-14NRSN1 -0,5791534 0,28199756 1,696933 1,4136827 1,40E-06 2,50E-08OPLAH -0,4595209 0,54160625 0,7647645 0,98544586 1,64E-04 1,63E-06PARM1 -0,561963 0,29335842 1,0755407 1,6686709 0,0062575 4,19E-05PDE1A 0,0060667 0,8034042 1,772167 1,8814553 0,01236653 8,08E-05
PDLIM1 0,0440392 0,76221764 0,848949 0,90552276 0,03332815 2,03E-04PDRG1 -0,0141821 0,9328816 1,690243 1,3890612 2,14E-04 2,03E-06
PELI2 0,2971584 0,8203451 2,2108323 1,2810482 4,86E-06 7,67E-08PHF20L1 0,0265927 0,87338585 2,2388226 1,9816772 0,00104408 8,53E-06PHYHIP -0,4811795 0,34839746 0,1275908 0,7137319 0,04021305 2,42E-04
PIGS -0,7857006 0,546718 -0,2474285 0,5909669 0,02786803 1,73E-04PKNOX2 -0,5035165 0,47361904 0,2723562 0,5461753 1,36E-05 1,89E-07
PLCB4 -0,2250915 0,48047394 1,199794 1,0307131 2,20E-05 2,87E-07PLK3 0,2149073 1,0056143 1,932799 1,6988721 0,00490319 3,36E-05POLB -0,0305873 0,86776924 1,384233 1,3096446 0,00179864 1,37E-05
PORCN -0,6983927 0,4565519 0,27228 0,8507489 6,93E-04 5,88E-06PPFIA3 -0,6428908 0,5045011 -0,0904475 0,61259514 0,0279556 1,72E-04PPP5C -0,7080577 0,44538105 0,165045 0,9334911 0,01292741 8,43E-05
PRKACB -0,5425087 0,50499827 0,4533283 0,99355614 0,00516126 3,50E-05PYGB -0,5423337 0,57642126 0,751398 1,0356414 1,51E-04 1,52E-06
RGS10 -0,3209165 0,7441539 1,4774944 1,3027033 2,41E-05 3,12E-07RHBDF1 -0,1925004 0,66763437 1,8359515 1,6502787 1,99E-04 1,91E-06RNASE1 -0,1858564 0,55607027 1,186209 1,5394343 0,02355307 1,47E-04ROBO1 0,509507 0,8565598 1,795602 1,0970726 5,29E-04 4,60E-06ROGDI -0,7804328 0,2900936 -0,387233 0,45719376 0,04102953 2,47E-04RPP25 -0,6690362 0,4288585 -0,140358 0,4847798 0,00190542 1,44E-05
RPS6KL1 -0,9170204 0,5666589 -0,013362 0,73695856 2,28E-04 2,15E-06RUSC1 -0,2462419 0,69223845 1,1333696 1,0263491 3,91E-05 4,78E-07
RYR2 -0,454788 0,4530526 0,4303975 1,037797 0,03991968 2,41E-04SDHA -0,713013 0,5599515 0,3531556 0,76818657 2,09E-05 2,76E-07
SEPT6 -0,7200754 0,39743847 1,076147 1,6961476 0,00226349 1,68E-05SERPINI1 -0,7030228 0,35907117 0,0997056 0,9537434 0,04580552 2,74E-04
SESN1 -0,435666 0,7455516 0,8936836 1,3014187 0,00411837 2,90E-05SGIP1 -0,56837 0,32829472 -0,2615173 0,34838316 0,04170696 2,50E-04
SLC17A5 -0,2659822 0,6200616 0,5158085 0,83521056 0,01610521 1,03E-04SLC22A15 -0,1662992 0,60187596 1,708103 1,6899402 0,00114985 9,28E-06
SLC35F3 -0,7217239 0,40244249 0,7692608 1,0323119 9,83E-06 1,44E-07SLC38A1 -0,5503948 0,8365302 0,1540708 0,64937586 0,0041854 2,93E-05SLC8A2 -0,281969 0,5725372 0,9984013 1,1584364 0,00119142 9,53E-06SMAD6 -0,4472466 0,61817366 0,2904102 0,5748161 1,27E-04 1,33E-06
Pöschl et al., Suppl. Table 3
SMPDL3A -0,7160996 0,36907792 0,0721707 0,6410513 2,09E-04 1,99E-06SPHKAP -0,370404 0,50993276 1,958851 1,8665496 1,50E-04 1,51E-06SREBF2 -0,2874797 0,8118976 0,431087 0,77924067 0,02783053 1,72E-04
ST6GAL2 -0,4354087 0,3143439 0,4002094 0,83106667 0,0049151 3,36E-05STAC -0,3915585 0,54841083 0,5266845 0,75820273 2,79E-04 2,58E-06STX7 -0,4811688 0,6374237 0,4373166 0,68424505 4,47E-05 5,25E-07SV2B -0,6847444 0,43778887 0,0233998 0,7096411 0,00574526 3,88E-05
TEK -0,4974058 0,60711986 0,8078179 1,1936158 0,00144554 1,13E-05TIAM1 -0,364796 0,3008619 0,167677 0,4439039 3,24E-04 2,89E-06
TLE4 -0,0979121 0,6937363 0,930536 1,0090116 0,00456998 3,15E-05TMEFF2 -0,350781 0,51214564 0,61375 0,9981121 0,00876284 5,82E-05
TMEM178A -0,3585647 0,71416587 0,8374557 1,1928674 0,00516723 3,51E-05TMEM56 -0,5048593 0,43631238 0,658014 1,2581904 0,01482112 9,56E-05
TNFAIP8L1 0,3791382 0,8114021 1,3439624 1,115844 0,04141621 2,49E-04TRHDE -0,4823251 0,8035727 1,5071465 1,6624619 3,14E-04 2,83E-06
TRIB1 -0,0975314 0,7613297 0,758401 0,6514806 9,37E-05 1,02E-06UQCRC1 -0,6515997 0,76806855 0,626607 1,1492009 0,00114908 9,23E-06YWHAB -0,755172 0,72642666 0,2095014 0,9353588 0,00414306 2,91E-05
ZIC2 0,0836005 0,65579444 1,6489164 0,6640178 1,30E-11 1,64E-12ZNF385B -0,5625945 0,37702718 -0,2201105 0,3808711 0,03544933 2,16E-04
High in human adult SHH and murine tumors
MEAN ADULT and MOUSE
Tumors
SD Adult and Mouse Tumors
Mean Infant Tumors
SD Infant Tumors
adjusted p-value FDR
ACER3 -0,0952256 1,011967 -0,957838 0,2881769 2,26E-10 1,64E-11AHRR 0,5981068 1,301876 0,01778 0,3198872 0,012237 8,02E-05
ANXA4 1,0743653 1,147776 0,087374 0,8064941 7,02E-04 5,92E-06APOM 0,3689296 1,008735 -0,351325 0,4778444 1,14E-04 1,20E-06
B4GALT4 -0,0480345 0,862099 -0,692056 0,3516372 4,34E-06 6,94E-08BEND7 0,940102 0,987179 -0,158313 0,5720118 1,58E-08 5,83E-10
BTBD11 1,091316 1,087677 -0,186072 0,5610309 7,00E-11 6,42E-12C12ORF65 0,4213464 1,07523 -0,545668 0,5418081 5,98E-07 1,16E-08C16ORF46 0,2460915 1,068398 -0,384951 0,5302064 0,0082143 5,47E-05C1ORF114 -0,2054475 0,84276 -0,628757 0,336676 0,0192801 1,22E-04C7ORF60 1,2616937 1,522562 -0,021688 0,7246883 1,46E-06 2,58E-08CACHD1 0,0536923 0,675923 -0,610066 0,1646029 0 0
CAHM 0,2224798 1,283444 -0,447186 0,536771 0,014838 9,59E-05CASP9 0,2412068 1,060102 -0,603843 0,5640832 4,52E-05 5,26E-07
CCDC113 0,0394992 1,139139 -0,640771 0,4554429 8,85E-04 7,30E-06CCDC88C -0,0407081 0,755089 -0,381261 0,2450627 0,0299966 1,84E-04
CCND1 1,444437 1,441189 -0,165493 0,2953426 0 0CDC42EP4 0,7632887 1,376525 -0,191658 1,0041623 0,0354765 2,16E-04
CDK20 -0,2420426 0,865941 -1,143827 0,4614403 1,74E-08 6,04E-10CETN2 0,4130892 1,092342 -0,453519 0,7378797 0,0018049 1,37E-05
CHRDL2 0,2464835 1,087594 -0,514763 0,3935759 6,14E-06 9,47E-08CKLF-CMTM1,CKLF 0,966255 0,823168 0,06648 0,6129114 1,11E-05 1,59E-07
CNTN3 0,4688856 1,362617 -0,456466 0,1536363 3,15E-08 9,67E-10CYB561 -0,443189 0,314315 -0,653358 0,1088157 1,39E-05 1,91E-07
CYP3A7-CYP3AP1 0,5417137 1,435156 -0,224892 0,4778651 0,0019719 1,48E-05DYX1C1 0,4147727 0,904297 -0,543846 0,5212942 7,04E-08 1,81E-09
EBF4 -0,1104117 0,862199 -0,780001 0,4759495 1,46E-04 1,50E-06EFS 1,180655 0,886495 0,078557 0,8123115 4,26E-05 5,08E-07
EMILIN2 0,07424 0,908357 -0,446843 0,3805681 0,0029577 2,14E-05EXTL3 -0,0833707 0,854009 -0,64975 0,4650104 0,0029526 2,14E-05
FAIM 0,4182827 0,910899 -0,251506 0,4863372 3,02E-04 2,77E-06FAM76A 0,4394886 0,976084 -0,291399 0,7204956 0,0139394 9,05E-05
FANCL 0,7750043 0,829993 -0,306906 0,7590218 1,45E-05 1,97E-07FBXO46 0,5583004 0,970048 -0,254534 0,7480789 0,0042003 2,93E-05
FGD4 -0,0637363 1,218802 -0,906401 0,207153 3,80E-08 1,11E-09FKBP9 0,346961 0,90628 -0,293627 0,5616908 0,004404 3,06E-05
Pöschl et al., Suppl. Table 3
FOXA2 0,3199777 0,971904 -0,357038 0,0280633 6,75E-09 3,15E-10FOXL2 0,4916586 1,435407 -0,21137 0,4931276 0,0114004 7,50E-05FOXQ1 0,0514593 0,889428 -0,313801 0,1387825 0,0172287 1,10E-04
GALNT1 0,458444 0,871609 -0,655611 0,6196032 4,94E-08 1,37E-09GAS2L1 -0,090239 0,957126 -0,88512 0,5374874 4,02E-05 4,88E-07
GCA 0,6086573 1,054532 -0,440584 0,5641242 8,64E-08 2,04E-09GCM1 0,5735888 1,279086 -0,50052 0,3112257 8,63E-11 6,72E-12
GGNBP2 -0,1151472 0,857138 -0,625835 0,4926461 0,0280362 1,73E-04GLIS2 0,9586489 1,139555 -0,520197 0,7917689 1,54E-08 5,89E-10
GMCL1 0,9196033 0,840332 0,262815 0,7240793 0,0415592 2,50E-04GPR85 1,4470918 1,123342 0,293114 0,8114678 2,60E-05 3,33E-07
GZF1 0,4602987 0,932817 -0,712238 0,7093162 4,69E-07 9,36E-09HOXA-AS4,MIR196B 0,6529936 1,474616 -0,416103 0,1998922 3,30E-09 1,77E-10
HOXA1 0,5031866 1,489294 -0,254 0,3981102 0,0015443 1,20E-05HOXA10 0,6317715 1,395936 -0,485327 0,0539474 1,87E-11 2,09E-12HOXA11 0,4322283 1,217073 -0,299653 0,2636619 9,90E-06 1,44E-07HOXA2 0,6660435 1,654942 -0,342502 0,0845849 8,07E-07 1,49E-08HOXA4 0,4752561 1,052226 -0,404863 0,1667724 8,41E-12 1,20E-12HOXC9 -0,1840945 0,490334 -0,510984 0,0361188 4,33E-08 1,23E-09HOXD3 -0,1827093 0,82528 -0,603989 0,0273359 1,10E-04 1,18E-06
HRASLS 0,0132176 0,82279 -0,618592 0,344511 2,82E-06 4,77E-08HSD11B2 1,4870064 1,158759 0,147834 0,6686629 3,91E-09 2,00E-10
HUNK -0,312809 0,633394 -0,599929 0,2046803 0,0275728 1,71E-04IFI27L2 -0,0739198 0,795994 -0,724342 0,4053974 1,15E-05 1,62E-07
IFT81 0,559194 1,023085 -0,506067 0,6280697 5,37E-07 1,05E-08KAT6B 0,9563817 1,480377 -0,054822 0,697697 2,91E-04 2,68E-06
KDM3A 0,7376887 0,761071 -0,424122 0,6990894 3,30E-07 6,82E-09KIAA1143 0,798632 0,994803 -0,069972 0,741492 0,0012959 1,03E-05L3MBTL3 0,2847604 0,937449 -0,752137 0,4468039 8,63E-11 7,26E-12
LAP3 1,1864674 1,02953 0,306468 0,8953609 0,0145491 9,43E-05LASP1 -0,1750205 0,859123 -1,054654 0,4440873 1,50E-08 5,95E-10
LCA5 0,8974945 1,075549 0,053365 0,6596208 5,60E-04 4,84E-06LDB3 0,2654933 1,255933 -0,263936 0,3379459 0,0377066 2,28E-04LHX2 0,0546727 0,692179 -0,765533 0,0968611 0 0
LOC100506963 0,4510414 0,960752 -0,508895 0,7118466 8,15E-05 9,10E-07LOC729059 0,262781 1,154645 -0,447227 0,652477 0,0153043 9,86E-05
LRRC8D 0,416706 0,868314 -0,34169 0,3862729 1,31E-07 2,86E-09LUC7L2 0,5303025 0,858776 -0,21047 0,767474 0,0175491 1,12E-04
MAP3K1 1,1092884 1,056861 -0,143627 0,6665403 1,86E-08 6,06E-10MAP3K7 0,6546888 0,797773 -0,208613 0,7853407 0,0019782 1,48E-05MARC1 0,3777475 1,154243 -0,361283 0,7110641 0,0194348 1,23E-04MDFIC 1,134877 1,248855 0,130066 0,5289025 7,95E-07 1,49E-08
NFATC1 0,8949742 0,989438 0,000715 0,7166965 4,28E-04 3,80E-06NHLRC2 0,6292607 0,902075 -0,490868 0,844819 6,26E-05 7,10E-07
NKAP 0,3475758 0,88059 -0,299814 0,5658765 0,0035399 2,52E-05NKX2-5 0,3983375 1,245536 -0,37102 0,244548 2,99E-06 4,91E-08NKX3-2 0,3186125 0,967768 -0,514778 0,4084792 7,93E-08 1,95E-09
NT5DC1 0,9685275 1,167992 0,150787 0,6487275 0,0013835 1,09E-05NUCB2 0,0755247 0,704866 -0,576815 0,3748322 7,65E-07 1,45E-08
OCRL -0,073078 0,893888 -0,735896 0,5433634 0,0014771 1,15E-05ONECUT1 0,6343161 1,597973 -0,21901 0,5070946 0,0014677 1,15E-05
OTX2 -0,2795478 1,105154 -1,13197 0,1635387 2,97E-10 2,01E-11PAN2 0,3823816 0,870212 -0,750166 0,5969486 1,07E-08 4,42E-10
PARD3 0,5063717 0,986804 -0,638582 0,5318242 4,45E-10 2,83E-11PAX5 -0,0346022 1,230216 -0,688136 0,0551955 4,25E-05 5,11E-07
PCDHB10,PCDHB9 0,0600051 0,875091 -0,630943 0,5338054 4,63E-04 4,04E-06PCDHB14 0,2034172 0,933378 -0,591328 0,3825078 6,76E-08 1,78E-09
PCGF5 0,6710183 0,992894 0,040571 0,4385573 6,72E-04 5,72E-06PDCD7 0,3367924 0,946307 -0,444676 0,7084231 0,0035663 2,53E-05PDE6B 0,021451 0,692546 -0,346484 0,1973341 8,65E-04 7,17E-06
PDLIM4 -0,1222025 0,77563 -0,438341 0,1342339 0,0224325 1,41E-04PHACTR1 -0,178674 0,557283 -0,619847 0,3066461 9,04E-05 9,87E-07
PHF17 -0,2005346 0,644414 -0,812363 0,4542049 9,56E-05 1,03E-06PHF19 0,2867364 0,86474 -0,364907 0,5422755 0,001668 1,28E-05PITX2 0,5171804 1,048851 -0,203359 0,4724459 1,47E-04 1,49E-06POLI 0,5635464 1,120879 -0,229633 0,653977 0,0020855 1,55E-05
POU4F2 -0,1072643 0,826568 -0,485153 0,1151774 0,0022891 1,69E-05PPCDC 0,245646 0,943732 -0,326455 0,5720534 0,0355465 2,16E-04
PUS7 0,4065213 0,869527 -0,231145 0,4452824 1,81E-04 1,77E-06RAD17 0,4818599 0,978546 -0,385033 0,9084581 0,0188629 1,20E-04
RALGPS2 0,2077333 0,962697 -0,368668 0,1942998 8,47E-06 1,29E-07RB1 1,0337054 0,976938 -0,062008 0,8505462 1,38E-04 1,44E-06
RBL2 0,2968916 0,905773 -0,325189 0,6432979 0,03066 1,88E-04RECQL 0,756507 1,110898 -0,223905 0,4834452 5,93E-08 1,60E-09RGAG4 0,2343118 0,940985 -0,441194 0,5671903 0,0024196 1,77E-05RIBC1 0,3001196 0,956242 -0,582118 0,7369257 7,82E-04 6,51E-06
RSRC1 0,4586014 0,775147 -0,25497 0,3188119 4,12E-09 2,01E-10RYK 0,8500287 0,839955 -0,131344 0,6937516 1,94E-05 2,62E-07
SIPA1L3 0,693761 0,931099 -0,361994 0,8754551 4,33E-04 3,82E-06SLC10A4 -0,2489188 0,74363 -0,754792 0,1273946 7,09E-08 1,79E-09
SLC26A11 -0,5622037 0,46589 -1,14466 0,2840126 1,21E-09 6,88E-11SMAD5 0,1673476 0,894725 -0,580813 0,5322102 8,87E-05 9,76E-07
SNX7 1,1021063 1,16926 0,219546 0,61549 1,40E-04 1,45E-06STK32C 0,2528254 1,083473 -0,815711 0,5347794 1,83E-08 6,14E-10STXBP3 0,2013184 1,240376 -0,448863 0,583995 0,0323643 1,98E-04
SWI5 -0,203258 0,753479 -0,753938 0,2661984 1,21E-06 2,19E-08TBX1 0,5090353 0,853923 -0,291625 0,6176972 1,99E-04 1,92E-06
THNSL1 0,700196 0,901092 -0,183279 0,8908826 0,0097308 6,43E-05TIPIN 0,8037402 0,795967 -0,5242 0,6029882 6,04E-11 6,08E-12
TMEM107 0,6419439 0,958967 -0,320631 0,5226639 1,01E-07 2,34E-09TMEM164 -0,1988593 0,84078 -0,561389 0,2569161 0,0431573 2,58E-04TMEM45A 0,1663927 0,891957 -0,306112 0,234917 6,11E-04 5,23E-06
TSHZ3 0,3707785 0,863036 -0,716207 0,0795852 0 0TSPAN14 0,7810885 1,194303 0,054398 0,753192 0,0477096 2,85E-04
TYW1B,TYW1 0,206088 0,984801 -0,845668 0,5542871 2,87E-08 9,08E-10UBE4A 0,2477583 1,118411 -0,748115 0,5036421 8,51E-08 2,05E-09
UNG 0,694907 0,867657 -0,354629 0,7172118 8,73E-06 1,31E-07VAMP7 0,0245935 0,772734 -0,65886 0,5769031 0,0010469 8,52E-06VGLL4 0,6344673 0,867071 -0,253393 0,7151102 3,13E-04 2,83E-06VWCE 0,5820996 1,331485 -0,155876 0,5453785 0,0045238 3,13E-05
WDR5B 0,3523528 1,225702 -0,632634 0,6838791 7,87E-05 8,86E-07WDSUB1 -0,0437685 1,027241 -0,662819 0,6388025 0,0476504 2,84E-04ZBTB20 0,8710315 1,050409 -0,348773 0,5744141 6,37E-10 3,81E-11ZCRB1 0,6153397 1,022732 -0,232889 0,6872098 7,20E-04 6,05E-06
ZKSCAN4 0,7067904 0,861156 -0,064777 0,65329 0,0010527 8,53E-06ZMYM2 0,22118 0,947893 -0,497022 0,7597044 0,029507 1,82E-04ZNF25 0,9285087 1,056975 -0,244179 0,7515834 2,75E-06 4,70E-08
ZNF274 0,3112697 0,844664 -0,679546 0,6308789 1,96E-06 3,40E-08ZNF397 0,0445938 0,872268 -0,645448 0,2904636 3,67E-08 1,10E-09ZNF438 0,703916 0,840858 -0,479737 0,874898 3,86E-05 4,77E-07ZNF503 0,4975666 0,884572 -0,350168 0,1825055 0 0
ZNF664-FAM101A,FAM101A -0,0140498 1,03962 -0,576498 0,1826772 8,49E-05 9,41E-07
Pöschl et al., Suppl. Table 3
Suppl. Table 3: 287 genes that are similarly expressed in adult human SHH medulloblastoma and murine Shh medulloblastoma mouse models and significantly differ from infantile human SHH medulloblastoma.