plant tissue culture basal salts and...

1

Plant Tissue Culture Basal Salts and MediaProduct Number Product Description Product Notes Package

SizeA267 ANDERSON BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by Anderson (1978, 1980).Plant Tissue Culture Tested

Storage TempSoluble In

2-6° CWater

1 L10 L50 L

B144 BANANA AGS BASAL MEDIUMContains the macro- and micronutrients, vitamins, and plant growth regulators required to culture bananas.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M462 Musa (Banana) Medium: See MS-Based Plant Specifi c Medium Section

C206 CAPE SUNDEW/ VENUS FLY TRAP MULTIPLICATION BASAL MEDIUMContains the macro- and micronutrients, vitamins, and plant growth regulators required for the multiplication of carnivorous plants.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C216 CAPE SUNDEW/ VENUS FLY TRAP PRETRANSPLANT BASAL MEDIUMContains the macro- and micronutrients, vitamins, and plant growth regulators required for the growth of carnivorous plants.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C212 CARROT CALLUS INITIATION BASAL MEDIUMContains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate carrot callus from pith tissue.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C222 CARROT SHOOT DEVELOPMENT BASAL MEDIUMContains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate shoots from carrot callus.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C287 CHEE & POOL C2d VITIS BASAL MEDIUMContains the macro- and micronutrients as described by Chee & Pool (1987).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C167 CHU N6 BASAL MEDIUM w/ VITAMINSContains the macro- and micronutrients, and vitamins as described by Chu (1975).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C416 CHU N6 BASAL SALT MIXTUREContains the macro- and micronutrients as described by Chu (1975).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

C149 CHU N6 VITAMIN SOLUTION (1000x)See Vitamin Section (following the MS-based media section)

D146 DCR BASAL SALT MIXTUREContains macro- and micronutrients as described by Gupta & Durzan (1985).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

Our Plant Tissue Culture Tested basal salts and media have been formulated according to the references

cited in their respective product listings. These basal salts and media are then manufactured and packaged in en-vironmentally controlled facilities. Our biological testing ensures that each lot produces culture growth consistent with prior lots.

On the following pages are many popular basal salt and media formulas followed by Murashige and Skoog-based formulations. Vitamin listings and formulations complete this section. Please contact us with inquiries about larger package sizes or custom formulations.

Instructions for basal salt, media, and vitamin preparation

are provided in the Technical Information Section of this catalog and on our web site: www.phytotechlab.com.

Basal Salt vs Medium... our defi nition A “Basal Salt Mixture” contains macro- and micronutri-B A “Basal Salt Mixture” contains macro- and micronutri-Bents, but no vitamins, carbohydrates, or growth regulators. A “Basal Medium” contains the macro- and micronutrients, but is incomplete as it typically lacks an organic compo-nent: vitamins, carbohydrates, or growth regulators. A “Medium” typically is complete and requires no additional components (except gelling agent, if desired). A “Modifi ed” Basal Salt, Basal Medium, or Medium indicates that one or more of the components varies in concentration or form from that originally published.

2

All components expressed in mg/L

And

erso

n B

asal

Sal

t Mix

ture

Ban

ana

AG

S B

asal

Med

ium

Cap

e S

unde

w/V

enus

Fly

Tra

p M

ultip

. Bas

al M

ediu

m

Cap

e S

unde

w/ V

enus

Fly

Tra

p P

retra

nspl

ant B

asal

Med

ium

Car

rot C

allu

s In

itiat

ion

Bas

al

Med

ium

Car

rot S

hoot

Dev

elop

men

t B

asal

Med

ium

Che

e &

Poo

l C2d

Viti

s B

asal

Med

ium

Chu

N6

Bas

al M

ediu

mw

/ Vita

min

s

Chu

N6

Bas

al S

alt M

ixtu

re

DC

R B

asal

Sal

t Mix

ture

COMPONENT A267 B144 C206 C216 C212 C222 C287 C167 C416 D146Aluminum Chloride•6H2OAmmonium Nitrate 400 1650 400 825 1650 400Ammonium Phosphate, MonobasicAmmonium Sulfate 134 134 463 463Boric Acid 6.2 6.2 6.2 3.1 3 3 6.2 1.6 1.6 6.2Calcium Chloride, Anhydrous 332.2 333 332.2 166.5 113.24 113.24 125.33 125.33 64.14Calcium Nitrate 492.3 386.31Cobalt Chloride•6H2O 0.025 0.025 0.025 0.0125 0.025 0.025 0.025 0.025Cupric Sulfate•5H2O 0.025 0.025 0.025 0.0125 0.025 0.025 0.025 0.25Na2EDTA•2H2O 74.5 74.5 37.26 37.26 37.3 37.25 37.25 37.3Ferric ChlorideFerric Sodium EDTA 36.7 18.35Ferrous Sulfate•7H2O 55.7 55.7 27.8 27.8 27.8 27.85 27.85 27.8Magnesium Sulfate, Anhydrous 180.7 181 180.7 90.5 122.09 122.09 180.6 90.37 90.37 180.7Manganese Chloride•4H2OManganese Sulfate•H2O 16.9 16.9 16.9 8.45 10 10 0.845 3.3 3.3 22.3Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.125 0.25 0.25 0.25 0.25Molybdenum TrioxideNickel Chloride•6H2O 0.025Nickel Sulfate•6H2OPotassium ChloridePotassium Iodide 0.3 0.83 0.415 0.75 0.75 0.8 0.8 0.83Potassium Nitrate 480 1900 480 950 2500 2500 1900 2830 2830 340Potassium Phosphate, Dibasic

Potassium Phosphate, Monobasic 170 85 170 400 400 170

Potassium SulfateSodium NitrateSodium Phosphate Monobasic 330.6 295 380 150 150Sodium SulfateZinc Nitrate•6H2OZinc Sulfate•7H2O 8.6 8.6 8.6 4.3 2 2 8.6 1.5 1.5 8.6Adenine Hemisulfate 80Glycine (Free Base) 22,4-Dichlorophenoxyacetic Acid 16-γ,γ-Dimethylallylaminopurine (2iP) 10 1Indole-3-acetic Acid 1Kinetin 0.2L(-)Malic Acidmyo-Inositol 100 100 50 100 100 10Nicotinic Acid (Free Acid) 1 1 1 0.5Pyridoxine•HCI 1 1 1 0.5Thiamine•HCI 0.4 0.4 0.2 10 10 1 1Grams of powder to prepare 1 liter 1.89 4.71 2.12 2.20 3.21 3.21 4.49 3.99 3.98 1.64pH±0.5 at RT 3.7 5.7 3.5 4.3 3.0 4.0 4.0 4.0 4.1 4.0

NS = No Specifi cation Established rev 11/2005

3

Plant Tissue Culture Basal Salts and Media (continued)Product Number Product Description Product Notes Package

SizeD190 DKW BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan, et al (1987).


2-6° CWater

1 L10 L50 L

D191 DKW BASAL MEDIUMContains 10 g/L Sucrose; without VitaminsContains macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan, et al. (1987).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

D189 DKW BASAL MEDIUM Contains 30 g/L Sucrose; without vitaminsContains macro- and micronutrients as described by Driver & Kuniyuki (1984) and McGranahan, et al. (1987).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

E330 ERIKSSON VITAMIN SOLUTION (1000x)See Vitamin Section (following the MS-based media section)

G768 GAMBORG BASAL SALT MIXTUREContains the macro- and micronutrients as described by Gamborg, et al. (1968).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

G398 GAMBORG B-5 BASAL MEDIUMContains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

G359 GAMBORG (PRL-4-DM) LONG BASAL MEDIUMContains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1966).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

G249 GAMBORG VITAMIN POWDER (1000x)See Vitamin Section (following the MS-based media section)

G219 GAMBORG VITAMIN SOLUTION (1000x)See Vitamin Section (following the MS-based media section)

G371 GRESSHOFF & DOY BASAL MEDIUMContains the macro- and micronutrients as described by Gresshoff & Doy (1974).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

H393 HELLER BASAL SALT MIXTUREContains the macro- and micronutrients as described by Heller (1953).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

H396 HELLER/ WHITE MODIFIED BASAL SALT MIXTUREContains the macro- and micronutrients as described by Heller (1953) and White (1963). Without Molybdenum TrioxidePlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

H353 HOAGLAND MODIFIED BASAL SALT MIXTUREContains Ferrous SulfateContains the macro- and micronutrients as described by Hoagland and Arnon (1938).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

HOSTA MediaSee MS-Based Plant-Specifi c Media (following this section)

K413 KAO & MICHAYLUK BASAL SALT MIXTUREContains the macro- and micronutrients as described by Kao & Michayluk (1975).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

K427 KAO & MICHAYLUK MODIFIED BASAL MEDIUMContains the macro- and micronutrients, vitamins, and organic acids as described by Kao & Michayluk (1975).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

K421 KAO & MICHAYLUK VITAMIN SOLUTION (100x)See Vitamin Section (following the MS-based media section)

4


DK

W B

asal

Sal

t M

ixtu

re

DK

W B

asal

M

ediu

m w

/ 10

g/L

Suc

rose

DK

W B

asal

M

ediu

m w

/ 30

g/L

Suc

rose

Gam

borg

Bas

al

Sal

t Mix

ture

Gam

borg

B-5

B

asal

Med

ium

Gam

borg

(PR

L-4-

DM

) Lon

g B

asal

Med

ium

Gre

ssho

ff &

Doy

B

asal

Med

ium

Hel

ler B

asal

Sal

t M

ixtu

re

Hel

ler/

Whi

te

Mod

ifi ed

Bas

al

Sal

t Mix

ture

Hoa

glan

d M

odifi

ed

Bas

al S

alt M

ixtu

re

Kao

& M

icha

yluk

B

asal

Sal

t Mix

ture

Kao

& M

icha

yluk

M

odifi

ed B

asal

M

ediu

m

COMPONENT D190 D191 D189 G768 G398 G359 G371 H393 H396 H353 K413 K427Aluminum Chloride•6H2O 0.0543 0.03Ammonium Nitrate 1416 1416 1416 1000 600 600Ammonium Phosphate, Monobasic 115.03

Ammonium Sulfate 134 134 200Boric Acid 4.8 4.8 4.8 3 3 3 0.3 1 1.24 2.86 3 3Calcium Chloride, Anhydrous 112.5 112.5 112.5 113.24 113.24 113.24 56.7 453 453Calcium Nitrate 1367 1367 1367 241.2 300 656.4Cobalt Chloride•6H2O 0.025 0.025 0.25 0.025 0.025 0.025Cupric Sulfate•5H2O 0.25 0.25 0.25 0.025 0.025 0.25 0.025 0.03 0.03 0.08 0.025 0.025

Na2EDTA•2H2O 45.4 45.4 45.4 37.26 37.26 186.0 37.25 3.35 37.26 37.26

Ferric Chloride 1Ferrous Sulfate•7H2O 33.8 33.8 33.8 27.8 27.8 139.0 27.85 25 2.5 27.85 27.85Magnesium Sulfate, AnhydrousMagnesium Sulfate, Anhydrous 361.49 361.49 361.49 122.09 122.09 122.09 17.099 122.1 351.63 240.76 146.55 146.55Manganese Chloride•4H2O 1.81Manganese Sulfate•H2O 33.5 33.5 33.5 10 10 132.0 1 0.0758 0.01 10 10Molybdic Acid (Sodium Salt)•2H2O 0.39 0.39 0.39 0.25 0.25 0.25 0.025 0.25 0.25Molybdenum Trioxide 0.016Nickel Chloride 0.03 0.03Nickel Sulfate•6H2O 0.005 0.005 0.005Potassium Chloride 300 65 750 65 300 300Potassium Iodide 0.75 0.75 0.75 0.8 0.01 0.01 0.75 0.75Potassium Nitrate 2500 2500 1000 1000 80 606.6 1900 1900Potassium Phosphate, Monobasic 265 265 265 300 170 170Potassium Sulfate 1559 1559 1559Sodium Nitrate 600Sodium Phosphate Monobasic 150 150 90.0 108.75 16.5Sodium Sulfate 200Zinc Nitrate•6H2O 17 17 17Zinc Sulfate•7H2O 2 2 3 0.3 1 1 0.22 2 2p-Aminobenzoic Acid 0.2 0.02L-Arginine (Free Base) 40L-Ascorbic Acid 0.4 2Asparagine 40D-Biotin 0.00025 0.2 0.01D-Calcium Pantothenate 0.4 1Choline Chloride 0.2 1Citric Acid (Free Acid) Anhydrous 40Cyancobalamin, Vit B12 0.02Folic Acid 0.015 0.4Fumaric Acid 40L-Glutamine 60Glycine (Free Base) 20 4L(-)-Maleic Acid 40L-Methionine 30.0myo-Inositol 100 100 10 100Nicotinamide 1Nicotinic Acid (Free Acid) 1 0.5 0.1L-Phenylalanine 20Pyridoxine•HCI 1 0.5 0.1 1Pyruvic Acid 20Ribofl avin 0.015 0.2Sucrose 10,000 30,000Thiamine•HCI 10 0.5 1 1L-Tryptophan 40Vitamin A 0.01Vitamin D3 0.01Grams of powder to prepare 1 liter 5.22 15.22 35.22 3.10 3.21 3.64 2.71 1.64 1.04 1.63 3.65 3.9pH±0.5 at RT 4.3 4.0 4.1 4.0 4.0 NS 3.9 4.9 4.7 4.5 4.0 4.4NS = No Specifi cation Established

rev 11/2005rev 11/2005

5

Product Number Product Description Product Notes Package

SizeL689 LINSMAIER & SKOOG BASAL MEDIUM

This medium may be sold as Murashige & Skoog Medium with Minimal Organics (MSMO) by some media suppliers.Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

L477 LINSMAIER & SKOOG MODIFIED BASAL MEDIUMpH Adjusted and BufferedThis medium may be sold as Murashige & Skoog Medium with Minimal Organics (MSMO) by some media suppliers.Contains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

L467 LINSMAIER & SKOOG BASAL MEDIUMw/ 30 g/L SUCROSE & 7 g/L AGARContains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

L452 LINSMAIER & SKOOG MODIFIED BASAL MEDIUMw/ 30 g/L SUCROSE & 7 g/L AGARpH Adjusted and BufferedContains the macro- and micronutrients, and vitamins as described by Linsmaier & Skoog (1965).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

L154 LLOYD & McCOWN WOODY PLANT BASAL SALT MIXTUREContains the WPM macro- and micronutrients as described by Lloyd & McCown (1981).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

L449 LLOYD & McCOWN WOODY PLANT BASAL MEDIUM w/ VITAMINSContains the WPM macro- and micronutrients, and vitamins as described by Lloyd & McCown (1981).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

L444 LLOYD & McCOWN WOODY PLANT MICRONUTRIENT MIXTUREContains the WPM micronutrients described by Lloyd & McCown (1981).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M419 MG BASAL SALT MIXTUREModifi ed Murashige & Skoog/ Gamborg Basal Salt MixturePlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

MURASHIGE & SKOOG BASAL SALTS & MEDIASee Murashige & Skoog-Based Basal Salts & Media Section (following this section)

N492 NB BASAL MEDIUMModifi ed Chu/ Gamborg Basal MediumContains the macronutrients as described by Chu (1975) and the micronutrients as described by Gamborg, et al. (1968).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

N613 NITSCH & NITSCH BASAL SALT MIXTUREContains the macro- and micronutrients as described by Nitsch & Nitsch (1969).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

N616 NITSCH & NITSCH BASAL MEDIUM w/ VITAMINSContains the macro- and micronutrients, and vitamins as described by Nitsch & Nitsch (1969).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

N608 NITSCH & NITSCH VITAMIN POWDER (1000x)See Vitamin Section (following the MS-based media section)

N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)See Vitamin Section (following the MS-based media section)

6


Lins

mai

er &

Sko

og

Bas

al M

ediu

m(M

SM

O)

Lins

mai

er &

Sko

og

Mod

ifi ed

Bas

al M

ediu

m

(MS

MO

)

Lins

mai

er &

Sko

og B

asal

M

ediu

m

Lins

mai

er &

Sko

og

Mod

ifi ed

Bas

al M

ediu

m,

Buf

fere

d &

pH

Adj

uste

d

Lloy

d &

McC

own

Woo

dy

Pla

nt B

asal

Sal

t Mix

ture

Lloy

d &

McC

own

Woo

dy

Pla

nt B

asal

Med

ium

Lloy

d &

McC

own

Woo

dy

Pla

nt M

icro

nutri

ent M

ixtu

re

MG

Bas

al S

alt M

ixtu

re

(Mod

ifi ed

MS

/Gam

borg

B

asal

Sal

t Mix

ture

)

NB

Bas

al M

ediu

m

Nits

ch &

Nits

ch B

asal

Sal

t M

ixtu

re

Nits

ch &

Nits

ch B

asal

M

ediu

m

COMPONENT L689 L477 L467 L452 L154 L449 L444 M419 N492 N613 N616Ammonium Nitrate 1650 1650 1650 1650 400 400 720 720Ammonium Sulfate 33.5 463Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 6.2 2.3 3 10 10Calcium Chloride, Anhydrous 332.2 332.2 332.2 332.2 72.5 72.5 72.5 111.36 125.33 166 166Calcium Nitrate 386 386Cobalt Chloride•6H2O 0.025 0.025 0.025 0.025 0.0125 0.025Cupric Sulfate•5H2O 0.025 0.025 0.025 0.025 0.25 0.25 0.25 0.0125 0.025 0.025 0.025Na2EDTA•2H2O 37.26 37.26 37.26 37.26 37.3 37.3 37.3 18.64 37.26 37.26 37.26Ferrous Sulfate•7H2O 27.8 27.8 27.8 27.8 27.85 27.85 27.85 13.9 27.8 27.8 27.8Magnesium Sulfate, Anhydrous 180.7 180.7 180.7 180.7 180.7 180.7 180.7 75.7 90.37 90.372 90.372Manganese Sulfate•H2O 16.9 16.9 16.9 16.9 22.3 22.3 22.3 6.7 10 18.9 18.9Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.125 0.25 0.25 0.25Potassium Hydroxide 100 100Potassium Iodide 0.83 0.83 0.83 0.83 0.4 0.75Potassium Nitrate 1900 1900 1900 1900 1100 2830 950 950Potassium Phosphate, Monobasic 170 170 170 170 170 170 170 42.5 400 68 68Potassium Sulfate 990 990Sodium Nitrate 437.8Sodium Phosphate Monobasic 32.6Zinc Sulfate•7H2O 8.6 8.6 8.6 8.6 8.6 8.6 8.6 2.7 2 10 10Agar 7000 7000D-Biotin 0.05Folic Acid 0.5Glycine (Free Base) 2 2MES (Free Acid) 1000 1000myo-Inositol 100 100 100 100 100 100 100Nicotinic Acid (Free Acid) 0.5 1 5Pyridoxine•HCI 0.5 1 0.5Sucrose 30,000 30,000Thiamine•HCI 0.4 0.4 0.4 0.4 1 10 0.5Grams of powder to prepare 1 liter 4.43 5.53 41.43 42.53 2.30 2.41 0.53 1.88 4.10 2.10 2.21pH±0.5 at RT NS 6.2 NS NS NS NS 4.3 4.0 3.8 NSNS = No Specifi cation Established rev 11/2005

7


SizeN479 NLN BASAL MEDIUM

Contains the macro- and micronutrients, and organic constituents as described by Lichter (1982); without sucrose.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

P713 PARKER THOMPSON BASIC C FERN BASAL SALT MIXTUREContains the macro- and micronutrients as described by Hickok, et al. (1995). Formulated for the growth of Ceratopteris richardii.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

Q673 QUOIRIN & LEPOIVRE BASAL SALT MIXTUREContains the macro- and micronutrients as described by Quoirin & Lepoivre (1977) and Quoirin, et al. (1977).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

R756 ROSE MODIFIED INITIATION BASAL MEDIUMStage IContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

R757 ROSE MODIFIED MULTIPLICATION BASAL MEDIUMStage IIContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

R758 ROSE MODIFIED ROOTING BASAL MEDIUMStage IIIContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

S816 SCHENK & HILDEBRANDT BASAL SALT MIXTUREContains the macro- and micronutrients as described by Schenk and Hildebrandt (1972).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

S811 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUMContains 10 g/L Sucrose; Without VitaminsPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

S808 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUM Contains 10 g/L sucrose, 1⁄2x vitamins, 1⁄2x micro- and 1⁄2x macronutrients.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

S806 SCHENK & HILDEBRANDT MODIFIED BASAL SALT MIXTURE Without CalciumPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

S813 SCHENK & HILDEBRANDT MODIFIED BASAL MEDIUMContains vitamins and 10g/L sucrose.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

S826 SCHENK & HILDEBRANDT VITAMIN POWDER (100x)See Vitamin Section (following the MS-based media section)

8


NLN

Bas

al M

ediu

m

Par

ker-

Thom

pson

Bas

ic C

Fe

rn B

asal

Sal

t Mix

ture

Quo

irin

& L

epoi

vre

Bas

al

Sal

t Mix

ture

Ros

e M

odifi

ed In

itiat

ion

Bas

al M

ediu

m

Ros

e M

odifi

ed

Mul

tiplic

atio

n B

asal

M

ediu

m

Ros

e M

odifi

ed R

ootin

g B

asal

Med

ium

Sch

enk

& H

ildeb

rand

t B

asal

Sal

t Mix

ture

Sch

enk

& H

ildeb

rand

t M

odifi

ed B

asal

Med

ium

Sch

enk

& H

ildeb

rand

t M

odifi

ed B

asal

Med

ium

Sch

enk

& H

ildeb

rand

t M

odifi

ed B

asal

Sal

t M

ixtu

re

Sch

enk

& H

ildeb

rand

t M

odifi

ed B

asal

Med

ium

COMPONENT N479 P713 Q673 R756 R757 R758 S816 S811 S808 S806 S813Ammonium Molybdate 0.037Ammonium Nitrate 125 400 1650 1650 412.5Ammonium Phosphate, Monobasic 300 300 150 300 300

Boric Acid 10 1.86 6.2 6.2 6.2 1.55 5.0 5.0 2.5 5.0 5.0Calcium Chloride, Anhydrous 19.628 333 333 83.25 151 151 75.5 151Calcium Nitrate 347 833.77Cobalt Chloride•6H2O 0.025 0.025 0.025 0.025 0.006 0.1 0.1 0.05 0.1 0.1Cupric Sulfate•5H2O 0.025 0.37 0.025 0.025 0.025 0.006 0.2 0.2 0.1 0.2 0.2Na2EDTA•2H2O 37.3 37.3 20 20 10 20 20Ferric Sodium EDTA 36.7 36.7 36.7 9.175Ferrous Sulfate•7H2O 27.8 27.8 15 15 7.5 15 15Magnesium Sulfate, Anhydrous 61 58.565 175.79 181 181 45.25 195.4 195.4 97.7 195.4 195.4Manganese Sulfate•H2O 18.95 0.25 0.76 16.9 16.9 4.225 10 10 5 10 10Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.25 0.063 0.1 0.1 0.05 0.1 0.1Potassium Iodide 0.08 0.83 0.83 0.208 1.0 1.0 0.5 1.0 1.0Potassium Nitrate 125 1800 1900 1900 475 2500 2500 1250 2500 2500Potassium Phosphate, Monobasic 125 500 270 170 170 42.5Zinc Sulfate•7H2O 10 0.52 8.6 8.6 8.6 2.15 1.0 1.0 0.5 1.0 1.0L-Ascorbic Acid 50 506-Benzylaminopurine (BA) 2.0 3.0D-Biotin 0.05Citric Acid (Free Acid) Anhydrous 50 50Folic Acid 0.5L-Glutamine 800Glutathion 30Glycine (Free Base) 2.0 2.0 2.0 2.0Indole-3-acetic Acid 0.30 0.30myo-Inositol 100 100 100 100 500 1000α-Naphthaleneacetic Acid 0.03Nicotinic Acid (Free Acid) 5.0 0.5 0.5 0.5 2.5 5.0Pyridoxine•HCI 0.5 0.5 0.5 0.5 0.25 0.5L-Serine 100Sucrose 10000 10000 10000Thiamine•HCI 0.5 0.4 0.4 0.4 2.5 5.0Grams of powder to prepare 1 liter 1.93 0.77 3.56 4.51 4.51 1.18 3.20 13.20 12.10 3.05 14.21pH±0.5 at RT 4.5 3.8 3.9 3.5 3.5 5.0 4.2 4.3 4.5 4.3 4.3NS = No Specifi cation Established

rev 11/2005

9


SizeT853 TM4G BASAL SALT MIXTURE

Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

T868 TM4G BASAL MEDIUM

Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

T856 TOBACCO MODIFIED CALLUS INITIATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

T864 TOBACCO MODIFIED SHOOT MULTIPLICATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

T867 TOBACCO MODIFIED SHOOT & ROOT BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

T861 TOBACCO MODIFIED ROOT INITIATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

W863 WESTVACO WV3 BASAL MEDIUMContains the macro- and micronutrients, and vitamins as described by Coke (1996b).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

W865 WESTVACO WV5 BASAL MEDIUMContains the macro- and micronutrients, and vitamins as described by Coke (1996a).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

W898 WHITE BASAL SALT MIXTUREContains the macro- and micronutrients as described by White (1963).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

Murashige & Skoog Basal Salts and Media following this section.

10


TM4G

Bas

al S

alt M

ixtu

re

TM4G

Bas

al M

ediu

m

Toba

cco

Mod

ifi ed

Cal

lus

Initi

atio

n B

asal

Med

ium

Toba

cco

Mod

ifi ed

Sho

ot

Mul

tiplic

atio

n B

asal

M

ediu

m

Toba

cco

Mod

ifi ed

Sho

ot &

R

oot B

asal

Med

ium

Toba

cco

Mod

ifi ed

Roo

t In

itiat

ion

Bas

al M

ediu

m

Wes

tvac

o W

V3

Bas

al

Med

ium

Wes

tvac

o W

V5

Bas

al

Med

ium

Whi

te B

asal

Sal

t Mix

ture

COMPONENT T853 T868 T856 T864 T867 T861 W863 W865 W898Ammonium Nitrate 320 320 1650 1650 1650 1650 700Ammonium Phosphate, Monobasic 230 230Ammonium Sulfate 130 130Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 31.0 31.0 1.5Calcium Chloride, Anhydrous 113.25 113.25 333 333 333 333 452.88 452.88Calcium Nitrate 208.5Cobalt Chloride•6H2O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025Cupric Sulfate•5H2O 0.025 0.025 0.025 0.025 0.025 0.025 0.25 0.25 0.001Na2EDTA•2H2O 18.65 18.65Ferric Sodium EDTA 36.7 36.7 36.7 36.7 36.7 36.71Ferric Sulfate 2.5Ferrous Sulfate 13.9 13.9Magnesium Sulfate, Anhydrous 122.12 122.12 181 181 181 181 903.79 903.79 351.62Manganese Sulfate•H2O 16.9 16.9 16.9 16.9 16.9 16.9 15.16 15.16 5.31Molybdenum Trioxide 0.001Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25Potassium Chloride 656.79 718.67 65.0Potassium Iodide 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.75Potassium Nitrate 1900 1900 1900 1900 1900 1900 910.06 1084.06 80.0Potassium Phosphate, Monobasic 170 170 170 170 270 270Sodium Phosphate Monobasic 16.5Sodium Sulfate 200Zinc Sulfate•7H2O 9.2 9.2 8.6 8.6 8.6 8.6 8.6 8.6 3.0D-Biotin 0.05Casein, Enzymatic Hydrolysate 1000 1000 1000 1000Folic Acid 0.5Glycine (Free Base) 2.5 2.0 2.0 2.0 2.0Indole-3-acetic Acid 2.0 0.03 3.0Kinetin 0.2 1.0 1.0myo-Inositol 100 100 100 100 100 1000 1000Nicotinic Acid (Free Acid) 5.0 0.5 0.5 0.5 0.5Pyridoxine•HCI 0.5 0.5 0.5 0.5 0.5Thiamine•HCI 0.5 0.4 0.4 0.4 0.4 0.4 0.4Grams of powder to prepare 1 liter 2.88 2.99 5.41 5.41 5.41 5.41 5.22 0.934pH±0.5 at RT NS NS 5.5 4.3 NS 5.0 NS NS 4.7NS = No Specifi cation Established rev 11/2005

11

Murashige & Skoog-Based Basal SaltsProduct Number Product Description Product Notes Package

SizeM524 MURASHIGE & SKOOG BASAL SALT MIXTURE

Contains the macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture TestedSee Product No. M519 for Murashige & Skoog Basal Medium w/ Vitamins


2-6° CWater

1 L10 L50 L

M499 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTUREContains FeNa–EDTAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M502 MURASHIGE & SKOOG MACRONUTRIENT SALT BASEContains the macronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M654 MURASHIGE & SKOOG MACRONUTRIENT STOCK SOLUTION (10x)Contains the macronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M554 MURASHIGE & SKOOG MICRONUTRIENT SALT BASEContains the micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M529 MURASHIGE & SKOOG MICRONUTRIENT STOCK SOLUTION (10x)Contains the micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M153 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTUREContains 1⁄2x macro- and 1⁄2x micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M561 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTUREContains 1⁄2x Ammonium Nitrate and 1⁄2x Potassium Nitrate, with the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M290 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTUREContains 1⁄2x Ammonium Nitrate, 1⁄2x Potassium Nitrate, and 1⁄2x Calcium Chloride, with the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M571 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTUREWithout Ammonium Nitrate. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M531 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTUREWithout Nitrogen. Contains the macro- and micronutrients as described by Murashige & Skoog (1962), modifi ed by eliminating NH4NO3 and KNO3.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M407 MURASHIGE & SKOOG MODIFIED BASAL SALT MIXTURE Without Nitrogen, Phosphorous, and PotassiumContains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

12


Mur

ashi

ge &

Sko

og (M

S)

Bas

al S

alt M

ixtu

re

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

w/ F

eNaE

DTA

MS

Mac

ronu

trien

t Sal

t Bas

e

MS

Mac

ronu

trien

t Sto

ck

Sol

utio

n (1

0x)

MS

Mic

ronu

trien

t Sal

t Bas

e

MS

Mic

ronu

trien

t Sto

ck

Sol

utio

n (1

0x)

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

(1/2

x M

icro

s &

Mac

ros)

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

(1/2

x N

itrat

es)

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

(No

NH

4NO

3)

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

(No

Nitr

ogen

)

MS

Mod

ifi ed

Bas

al S

alt

Mix

ture

(No

N, P

, or K

)

COMPONENT M524 M499 M502 M654 M554 M529 M153 M561 M290 M571 M531 M407

Ammonium Nitrate 1650 1650 1650 16500 825 825 825

Boric Acid 6.2 6.2 6.2 62 3.1 6.2 6.2 6.2 6.2 6.2

Calcium Chloride, Anhydrous 332.2 333 332.2 3322 166.1 332.2 166.1 332.2 332.2 332.2

Cobalt Chloride•6H2O 0.025 0.025 0.025 0.25 0.0125 0.025 0.025 0.025 0.025 0.025

Cupric Sulfate•5H2O 0.025 0.025 0.025 0.25 0.0125 0.025 0.025 0.025 0.025 0.025

Na2EDTA•2H2O 37.26 37.26 373 18.63 37.26 37.26 37.26 37.26 37.26

Ferric Sodium EDTA 36.7

Ferrous Sulfate•7H2O 27.8 27.8 278 13.9 27.8 27.8 27.8 27.8 27.8

Magnesium Sulfate, Anhydrous 180.7 181 180.7 1810 90.35 180.7 180.7 180.7 180.7 180.7

Manganese Sulfate•H2O 16.9 16.9 16.9 169 8.45 16.9 16.9 16.9 16.9 16.9

Molybdic Acid (Sodium Salt)•2H2O

0.25 0.25 0.25 2.5 0.125 0.25 0.25 0.25 0.25 0.25

Potassium Iodide 0.83 0.83 0.83 8.30 0.415 0.83 0.83 0.83 0.83 0.83

Potassium Nitrate 1900 1900 1900 19000 950 950 950 1900

Potassium Phosphate, Monobasic 170 170 170 1700 85 170 170 170 170

Zinc Sulfate•7H2O 8.6 8.6 8.6 86 4.3 8.6 8.6 8.6 8.6 8.6

Grams of powder to prepare 1 liter 4.33 4.30 4.23 N/A 0.10 NA 2.17 2.56 2.39 2.68 0.78 0.61

pH±0.5 at RT 3.9 NS NS 4.3 4.3 3.2 NS 4.3 NS NS 4.3 4.3

NS = No Specifi cation Established Rev. 11/2005

13

Murashige & Skoog-Based MediaProduct Number Product Description Product Notes Package

SizeM519 MURASHIGE & SKOOG BASAL MEDIUM w/ VITAMINS

With the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962).Plant Tissue Culture TestedContains the basal salts of Product No. M524


2-6° CWater

1 L10 L50 L

M541 MURASHIGE & SKOOG MODIFIED BASAL MEDIUMWithout Potassium Phosphate; contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients, and modifi ed vitamins as described by Murashige & Skoog (1962). Also contains (mg/L): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate, and 1000 Casein Hydrolysate.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M404 MURASHIGE & SKOOG MODIFIED BASAL MEDIUM w/ GAMBORG VITAMINSWith the macro- and micronutrients as described by Murashige & Skoog (1962), and vitamins as described by Gamborg, et al. (1966).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M401 MURASHIGE & SKOOG MODIFIED MEDIUMContains the macro- and micronutrients as described by Murashige & Skoog (1962); modifi ed with the addition of (mg/L): 1.0 BA and 0.1 NAAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M701 MURASHIGE & SKOOG MODIFIED BASAL MEDIUMThis medium was formerly listed as M506.This medium may be sold as Murashige Begonia Multiplication Medium by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modifi ed with the addition of (mg/L): 10 2iP and 30 Adenine Hemisulfate.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M702 MURASHIGE & SKOOG MODIFIED MEDIUMContains the macro- and micronutrients as described by Murashige & Skoog (1962); modifi ed with the addition of (mg/L): 30 2iP and 80 Adenine Hemisulfate, and 0.3 IAAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M535 MURASHIGE & SKOOG MODIFIED BASAL MEDIUMWithout Nicotinic Acid, Pyridoxine•HCl, and GlycineContains the macro- and micronutrients as described by Murashige & Skoog (1962); modifi ed with the addition of (mg/L) 0.4 Thiamine•HCl, 100 myo-Inositol, and 80 Adenine Hemisulfate.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

MURASHIGE MEDIUM WITH MINIMAL ORGANICS (MSMO)See L689 & L477 Linsmaier & Skoog Basal Medium

M536 MURASHIGE MODIFIED MULTIPLICATION BASAL MEDIUMContains the macro- and micronutrients, and vitamins as described by Murashige & Skoog (1962); modifi ed with the addition of (mg/L): 2.0 2iP, 80 Adenine Hemisulfate, and 170 Sodium Phosphate.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M555 MURASHIGE & SKOOG MODIFIED MULTIPLICATION MEDIUMThis medium may be sold as Murashige & Skoog Shoot Multiplication Medium C by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962); modifi ed with the addition of (mg/L): 1.0 Kinetin, 80 Adenine Hemisulfate, and 148 Sodium Phosphate Monobasic. Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M527 MURASHIGE MODIFIED MULTIPLICATION BASAL MEDIUMThis medium is sold as Murashige Shoot Tip Rooting Medium by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and vitamins as described by Linsmaier & Skoog (1965); modifi ed with the addition of (mg/L): 0.3 IAA, 1.0 Kinetin, and FeNaEDTA.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M491 MURASHIGE MODIFIED SHOOT MULTIPLICATION BASAL MEDIUMThis medium is sold as MS Shoot Multiplication Medium A by some media suppliers. Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and vitamins as described by Linsmaier & Skoog (1965); modifi ed with the addition of (mg/L): 170 Sodium Phosphate Monobasic, 80 Adenine Hemisulfate, 30 2iP, and 0.3 IAA.Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

MURASHIGE & SKOOG VITAMIN POWDERS & SOLUTIONSSee Vitamin Section (following this section)

14


Mur

ashi

ge &

Sko

og B

asal

M

ediu

m

MS

Mod

ifi ed

Bas

al M

ediu

m

(w/o

ut K

H2P

O4)

MS

Mod

ifi ed

Bas

al M

ediu

mw

/ Gam

borg

Vita

min

s

MS

Mod

ifi ed

Med

ium

w/ B

A &

NA

A

MS

Mod

ifi ed

Bas

al M

ediu

m

w/ 2

iP

MS

Mod

ifi ed

Med

ium

w/ 2

iP

& IA

A

MS

Mod

ifi ed

Bas

al M

ediu

m

Mur

ashi

ge M

odifi

ed

Mul

tiplic

atio

n B

asal

Med

ium

w

/ 2iP

MS

Mod

ifi ed

Mul

tiplic

atio

n M

ediu

m w

/ Kin

etin

Mur

ashi

ge M

odifi

ed

Mul

tiplic

atio

n B

asal

Med

ium

w

/ Kin

etin

& IA

A

Mur

ashi

ge M

odifi

ed S

hoot

M

ultip

licat

ion

Bas

al M

ediu

m

COMPONENT M519 M541 M404 M401 M701 M702 M535 M536 M555 M527 M491Ammonium Nitrate 1650 1650 1650 1650 1650 1650 1650 1650 1650 1650 1650Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2Calcium Chloride, Anhydrous 332.2 332.2 332.2 332.2 333 332.2 332.2 332.2 332.2 333 333Cobalt Chloride•6H2O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025Cupric Sulfate•5H2O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025Na2EDTA•2H2O 37.26 37.26 37.26 37.26 37.26 37.26 37.26Ferric Sodium EDTA 36.7 36.7 36.7 36.7Ferrous Sulfate•7H2O 27.8 27.8 27.8 27.8 27.8 27.8 27.8Magnesium Sulfate, Anhydrous 180.7 180.7 180.7 180.7 181 180.7 180.7 180.7 180.7 181 181Manganese Sulfate•H2O 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25Potassium Iodide 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83Potassium Nitrate 1900 1900 1900 1900 1900 1900 1900 1900 1900 1900 1900Potassium Phosphate, Monobasic 170 170 170 170 170 170 170 170 170 170Sodium Phosphate Monobasic 300 148 170 148 170Zinc Sulfate•7H2O 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6Adenine Hemisulfate 150 30 80 80 80 80 806-Benzylaminopurine (BA) 1Casein, Enzymatic Hydrolysate 1000Glycine (Free Base) 2 2 2 2.0

6-γ,γ-Dimethylallylaminopurine (2iP) 10 30 30

Indole-3-acetic Acid 1 0.3 0.3 0.3Indole-3-butyric AcidKinetin 1 1myo-Inositol 100 100 100 100 100 100 100 100 100 100 100α-Naphthaleneacetic Acid 0.1 0.1Nicotinic Acid (Free Acid) 0.5 5 1 0.5 0.5Pyridoxine•HCI 0.5 1 1 0.5 0.5Sucrose 30000 30000 30000Thiamine•HCI 0.1 0.5 10 0.4 0.4 0.4 0.4 0.4 0.4 0.4 0.4Grams of powder to prepare 1 liter 4.43 5.69 4.44 34.44 4.44 34.69 4.51 4.68 34.66 4.41 4.68pH±0.5 at RT 3.9 4.0 4.0 4.0 3.9 3.8 4.0 3.5 4.4 NS NSNS = No Specifi cation Established Rev 11/2005

15

MS-Based Plant-Specifi c MediaProduct Number Product Description Product Notes Package

SizeM517 MURASHIGE MODIFIED AFRICAN VIOLET/ GLOXINIA

MULTIPLICATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M518 MURASHIGE MODIFIED AFRICAN VIOLET/ GLOXINIA PRETRANSPLANT BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M550 MURASHIGE & SKOOG MODIFIED MEDIUMArabidopsis Culture MediumModifi ed with the addition of (mg/L): 2.0 2,4-D, and 0.05 KinetinPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M506 MURASHIGE BEGONIA MULTIPLICATION MEDIUMSee Product No. M701 Murashige & Skoog Modifi ed Medium (same formulation)

M508 MURASHIGE MODIFIED FERN MULTIPLICATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M509 MURASHIGE MODIFIED GERBERA MULTIPLICATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M510 MURASHIGE MODIFIED GERBERA PRETRANSPLANT BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

H435 HOSTA INITIATION/ MULTIPLICATION MEDIUMStage I/II MediumModifi ed Murashige & Skoog (1962) with (mg/L): 2.0 BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, and 0.5 NAA Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

H436 HOSTA MULTIPLICATION MEDIUMStage II MediumModifi ed Murashige & Skoog (1962) with (mg/L): 0.1 BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, and 0.5 NAAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

H437 HOSTA ROOTING MEDIUMStage III MediumModifi ed Murashige & Skoog (1962) with (mg/L): 0.1 BA, 500 Casein Hydrolysate, and 0.5 NAAPlant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

16


Mur

ashi

ge M

odifi

ed A

frica

n Vi

olet

/ G

loxi

nia

Mul

tiplic

atio

n B

asal

Med

ium

Mur

ashi

ge M

odifi

ed A

frica

n Vi

olet

/ G

loxi

nia

Pre

trans

plan

t Bas

al M

ediu

m

MS

Mod

ifi ed

Med

ium

(Ara

bido

psis

)

Mur

ashi

ge M

odifi

ed F

ern

Mul

tiplic

atio

n B

asal

Med

ium

Mur

ashi

ge M

odifi

ed G

erbe

ra

Mul

tiplic

atio

n B

asal

Med

ium

Mur

ashi

ge M

odifi

ed G

erbe

ra

Pre

trans

plan

t Bas

al M

ediu

m

Hos

ta In

itiat

ion/

Mul

tiplic

atio

n M

ediu

m

Hos

ta M

ultip

licat

ion

Med

ium

Hos

ta R

ootin

g M

ediu

m

Mur

ashi

ge &

Sko

og B

asal

Med

ium

(P

ublis

hed

Form

ula

Ref

eren

ce)

COMPONENT M517 M518 M550 M508 M509 M510 H435 H436 H437 M519Ammonium Nitrate 1650 1650 1650 1650 1650 1650 1650 1650 1650 1650Boric Acid 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2 6.2Calcium Chloride, Anhydrous 333 333 332.2 333 333 333 332.2 332.2 332.2 332.2Cobalt Chloride•6H2O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025Cupric Sulfate•5H2O 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025 0.025Na2EDTA•2H2O 37.26 37.26 37.26 37.26 37.26Ferric Sodium EDTA 36.7 36.7 36.7 36.7 36.7Ferrous Sulfate•7H2O 27.8 27.8 27.8 27.8 27.8Magnesium Sulfate, Anhydrous 181 181 180.7 181 181 181 180.7 180.7 180.7 180.7Manganese Sulfate•H2O 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9 16.9Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25Potassium Iodide 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83 0.83Potassium Nitrate 1900 1900 1900 1900 1900 1900 1900 1900 1900 1900Potassium Phosphate, Monobasic 170 170 170 170 170 170 300 300 300 170Sodium Phosphate Monobasic 170 255 85 85 170 170 170Zinc Sulfate•7H2O 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6 8.6Adenine Hemisulfate 80 80 160 160Agar 8000 8000 80006-Benzylaminopurine (BA) 2.0 0.1 0.1Casein, Enzymatic Hydrolysate 500 500 500Glycine (Free Base) 2.0 2.0 2.02,4-Dichlorophenoxyacetic Acid 2.0Indole-3-acetic Acid 2.0 1.0 0.5 10Indole-3-butyric AcidKinetin 2.0 0.05 2.0 10MES (Free Acid)myo-Inositol 100 100 100 100 100 100 100 100 100 100a-Naphthaleneacetic Acid 0.1 0.5 0.5 0.5Nicotinic Acid (Free Acid) 1.0 10 10 0.5Peptone from MeatPyridoxine•HCI 1.0 1.0 1.0 0.5Sucrose 20000 30000 30000 30000Thiamine•HCI 0.4 0.4 10 0.4 30 30 0.4 0.4 0.4 0.1L-Tyrosine 100 100Grams of powder to prepare 1 liter 4.66 4.4 24.44 4.66 4.72 4.64 43.40 43.39 43.23 4.43pH±0.5 at RT 4.0 4.8 4.0 4.5 NS 5.9 4.3 4.3 4.3 3.9NS = No Specifi cation Established Rev 11/2005

17

MS-Based Plant-Specifi c Media (continued)Product Number Product Description Product Notes Package

SizeM511 MURASHIGE MODIFIED KALANCHOE MULTIPLICATION BASAL MEDIUM

Contains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M512 MURASHIGE MODIFIED KALANCHOE PRETRANSPLANT BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M513 MURASHIGE MODIFIED LILY MULTIPLICATION BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M462 MUSA (BANANA) MULTIPLICATION MEDIUMIITA Formulation as described by Vuylsteke (1998).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L

M516 MURASHIGE & SKOOG MODIFIED BC POTATO BASAL MEDIUMContains FeNaEDTA in place of Ferrous Sulfate and Disodium EDTA. Contains the remaining macro- and micronutrients as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

1 L10 L50 L


Mur

ashi

ge

Mod

ifi ed

K

alan

choe

M

ult-

plic

atio

n B

asal

M

ediu

m

Mur

ashi

ge

Mod

ifi ed

K

alan

choe

P

re-

trans

plan

t B

asal

M

ediu

m

Mur

ashi

ge

Mod

ifi ed

Li

ly M

ulti-

plic

atio

n B

asal

M

ediu

m

Mus

a (B

anan

a)

Mul

ti-pl

icat

ion

Med

ium

MS

M

odifi

ed

BC

Pot

ato

Bas

al

Med

ium

COMPONENT M511 M512 M513 M462 M516Ammonium Nitrate 1650 1650 1650 1650 1650

Boric Acid 6.2 6.2 6.2 6.2 6.2

Calcium Chloride, Anhydrous 333 333 333 332.2 333

Cobalt Chloride•6H2O 0.025 0.025 0.025 0.025 0.025

Cupric Sulfate•5H2O 0.025 0.025 0.025 0.025 0.025

Na2EDTA•2H2O 37.25

Ferric Sodium EDTA 36.7 36.7 36.7 36.7

Ferrous Sulfate•7H2O 27.85

Magnesium Sulfate, Anhydrous 181 181 181 180.74 181

Manganese Sulfate•H2O 16.9 16.9 16.9 16.9 16.9

Molybdic Acid (Sodium Salt)•2H2O 0.25 0.25 0.25 0.25 0.25

Potassium Iodide 0.83 0.83 0.83 0.83 0.83

Potassium Nitrate 1900 1900 1900 1900 1900

Potassium Phosphate, Monobasic 170 170 170 170 170

Zinc Sulfate•7H2O 8.6 8.6 8.6 8.6 8.6

L-Ascorbic Acid 20

6-Benzylaminopurine (BA) 4.5

Gelrite 2000

D-Glucose 2.0

Glycine (Free Base) 2.0 2.0

6-γ,γ-Dimethylallylaminopurine (2iP) 3.0

Indole-3-acetic Acid 3.0 0.175

Kinetin 0.04

myo-Inositol 100 100 100 100

α-Naphthaleneacetic Acid 0.03

Nicotinic Acid (Free Acid) 0.5 0.5

Pyridoxine•HCI 0.5 0.5

Sucrose 30,000

Thiamine•HCI 0.4 0.4 0.4 0.4 0.4

Grams of powder to prepare 1 liter 4.41 4.41 4.4 36.36 4.41

pH±0.5 at RT 4.8 4.8 4.8 4.3 NSNS = No Specifi cation Established

18

VitaminsProduct Number Product Description Product Notes Package

SizeC149 CHU N6 VITAMIN SOLUTION (1000x)

Contains the vitamins as described by Chu (1975).Plant Tissue Culture Tested


2-6° CWater

100 mL

E330 ERIKSSON VITAMIN SOLUTION (1000x)Contains the vitamins as described by Eriksson (1965).Plant Tissue Culture Tested


2-6° CWater

100 mL

G249 GAMBORG VITAMIN POWDER (1000x)Contains the vitamins as described by Gamborg, et al. (1968).Plant Tissue Culture Tested


2-6° CWater

100 mL

G219 GAMBORG VITAMIN SOLUTION (1000x)Contains the vitamins as described by Gamborg, et al. (1968).Plant Tissue Culture Tested


2-6° CWater

100 mL

K421 KAO & MICHAYLUK VITAMIN SOLUTION (100x)Contains the vitamins described by Kao & Michayluk (1975).Plant Tissue Culture Tested


2-6° CWater

100 mL500 mL

1 L

M533 MURASHIGE & SKOOG VITAMIN POWDER (1000x)Contains the vitamins as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

100 mL250 mL

M553 MURASHIGE & SKOOG VITAMIN SOLUTION (1000x)Contains the vitamins as described by Murashige & Skoog (1962).Plant Tissue Culture Tested


2-6° CWater

100 mL

M547 MURASHIGE & SKOOG MODIFIED VITAMIN POWDER (1000x)Murashige & Skoog (1962) vitamins modifi ed with additional Thiamine•HCl.Plant Tissue Culture Tested

100 mL250 mL

M557 MURASHIGE & SKOOG MODIFIED VITAMIN SOLUTION (1000x)Murashige & Skoog (1962) vitamins modifi ed with additional Thiamine•HCl.Plant Tissue Culture Tested


2-6° CWater

100 mL

N608 NITSCH & NITSCH VITAMIN POWDER (1000x)Contains the vitamins as described by Nitsch & Nitsch (1969).Plant Tissue Culture Tested


2-6° CWater

100 mL250 mL

N603 NITSCH & NITSCH VITAMIN SOLUTION (1000x)Contains the vitamins as described by Nitsch & Nitsch (1969).Plant Tissue Culture Tested


2-6° CWater

100 mL

S826 SCHENK & HILDEBRANDT VITAMIN POWDER (100x)Contains the vitamins as described by Schenk & Hildebrandt (1972).Plant Tissue Culture Tested


2-6° CWater

100 mL1 L


Chu

N6

Vita

min

S

olut

ion

(100

0x)

Erik

sson

Vita

min

S

olut

ion

(100

0x)

Gam

borg

Vita

min

P

owde

r (10

00x)

Gam

borg

Vita

min

S

olut

ion

(100

0x)

Kao

& M

icha

yluk

Vi

tam

in S

olut

ion

(100

x)

MS

Vita

min

P

owde

r (10

00x)

MS

Vita

min

S

olut

ion

(100

0x)

MS

Mod

ifi ed

Vi

tam

in P

owde

r (1

000x

)

MS

Mod

ifi ed

Vi

tam

in S

olut

ion

(100

0x)

Nits

ch &

Nits

ch

Vita

min

Pow

der

(100

0x)

Nits

ch &

Nits

ch

Vita

min

Sol

utio

n (1

000x

)

Sch

enk

&

Hild

ebra

ndt

Vita

min

Pow

der

(100

x)COMPONENT C149 E330 G249 G219 K421 M533 M553 M547 M557 N608 N603 S826

p-Aminobenzoic Acid 2.0L-Ascorbic Acid 200D-Biotin 1.0 50 50D-Calcium Pantothenate 100Choline Chloride 100Cyancobalamin, Vit B12 2.0Folic Acid 40.0.0 500 500Glycine (Free Base) 2000 2000 2000 2000 2000 2000 2000 2000myo-Inositol 100000 100000 10000 100000 100000 100000 100000 100000 100000 100000Nicotinamide 100Nicotinic Acid (Free Acid) 500 500 1000 1000 500 500 500 500 5000 5000 500Pyridoxine•HCl 500 500 1000 1000 100 500 500 500 500 500 500 50.0Ribofl avin 20.0Thiamine•HCI 1000 500 10000 10000 100 100 100 1000 1000 500 500 500Vitamin A 1.0Grams of powder to prepare 1 L N/A N/A 112 N/A N/A 103.1 N/A 104 N/A 108.55 N/A 101.05

Rev 11/2005

plant tissue culture basal salts and...

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