plant pathogen booklet

PLANT PATHOGEN DETECTION

Aspergillus specie fungal conidiospores Page 4

www.norgenbiotek.com

ISO

9001:2008

ISO

13485:2003

ISO

15189:2012

Ordering Information

Website: www.norgenbiotek.com

Phone: 905-227-8848

Toll Free in North America: 1-866-667-4362

Fax: 905-227-1061

Email: [email protected]

Address:

Norgen Biotek Corp.

3430 Schmon Parkway

Thorold, ON

L2V 4Y6

CANADA

PLANT PATHOGEN DETECTION

Principle of the Test

Norgen’s PCR Detection Kits constituent a ready-to-use system for the isolation and detection

of Plant pathogens using end-point or real-time PCR. The kits first allows for the isolation of RNA

or DNA from samples using spin-column chromatography based on Norgen’s proprietary

resin. The RNA or DNA is isolated free from inhibitors, and can then be used as the template in

a PCR or one step RT-PCR reaction for pathogen detection using the provided Detection

Mastermix. The Detection Mastermix contains reagents and enzymes for the specific

amplification of a region of the target genome or RNA.

In addition, Norgen’s PCR Detection Kits contain a second Mastermix, the RT-PCR or PCR

Control Master Mix, which can be used to identify possible PCR inhibition and/or inadequate

isolation via a separate RT-PCR reaction with the use of the provided PCR control (PCRC) or

Isolation Control (IsoC), respectively. The kits are designed to allow for the testing of 24

samples.

Key Features

Rapid isolation of high quality RNA or DNA from challenging plant tissues or culture.

High sensitivity and specificity

Primer set and controls also available separately

Ideal for use in:

1. Identification of plant pathogen (Research only)

2. Epidemiologial Studies

3. Field Surveillance of Pathogens

4. Crop, vegetable and fruit quality assessment

End-Point PCR Real-Time PCR (SYBR® Green) Real-Time PCR (TaqMan®)

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mailto:[email protected]

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Product Description Cat. # Page

Aspergillus niger

PCR Detection Kit

Isolation and detection of Aspergillus niger DNA

from plant tissue and fruits. 32900 4

Botrytis cinerea

PCR Detection Kit

Isolation and detection of Botrytis cinerea DNA


Cladosporium cladosporioides

PCR Detection Kit

Isolation and detection of Cladosporium

cladosporioides DNA from plant tissue and fruits. 33000 6

Penicillium sp.

PCR Detection Kit

Isolation and detection of Penicillium sp. DNA


Saccharomyces cerevisiae

PCR Detection Kit

Isolation and detection of Saccharomyces

cerevisiae DNA from plant tissue, fruits and

beverage samples including wine and juice.

33300 8

Plum Pox Virus

RT-PCR Detection Kit

Isolation and detection of Plum Pox Virus RNA

from plant tissue, fruits and sap. 33400 9

Phomopsis viticola

CR Detection Kit

Isolation and detection of Phomopsis

viticola from plant tissues and fruits. 34900 10

Erwinia amylovora

PCR Detection Kit

Isolation and detection of Erwinia

amylovora from plant tissue. 35100 11

TABLE OF CONTENTS

https://norgenbiotek.com/product/aspergillus-niger-pcr-detection-kit

https://norgenbiotek.com/product/aspergillus-niger-pcr-detection-kit

https://norgenbiotek.com/product/botrytis-cinerea-pcr-detection-kit

https://norgenbiotek.com/product/botrytis-cinerea-pcr-detection-kit

https://norgenbiotek.com/product/cladosporium-cladosporioides-pcr-detection-kit

https://norgenbiotek.com/product/cladosporium-cladosporioides-pcr-detection-kit

https://norgenbiotek.com/product/penicillium-sp-pcr-detection-kit

https://norgenbiotek.com/product/penicillium-sp-pcr-detection-kit

https://norgenbiotek.com/product/saccharomyces-cerevisiae-pcr-detection-kit

https://norgenbiotek.com/product/saccharomyces-cerevisiae-pcr-detection-kit

https://norgenbiotek.com/product/plum-pox-virus-rt-pcr-detection-kit

https://norgenbiotek.com/product/plum-pox-virus-rt-pcr-detection-kit

https://norgenbiotek.com/product/phomopsis-viticola-pcr-detection-kit

https://norgenbiotek.com/product/phomopsis-viticola-pcr-detection-kit

https://norgenbiotek.com/product/erwinia-amylovora-pcr-detection-kit

https://norgenbiotek.com/product/erwinia-amylovora-pcr-detection-kit

Figure 1. Detection of A. niger using the A. niger PCR Detection

Kit. A representative 1X TAE 1.5% agarose gel showing the

amplification of A. niger positive (lane 1 and 2) negative (lane 3

and 4) controls. The size of the A. niger target amplicon

corresponds to 363 bp as represented by the provided DNA

Marker (M).

A ready-to-use system for the isolation and

detection of A. niger using end-point PCR

Norgen’s Aspergillus niger PCR Detection Kit is a ready-to-

use system for the isolation and detection of A. niger from

plant samples. First, the kit contains components for the

rapid isolation of total DNA, including fungal DNA, from

plant samples using spin-column chromatography based

on Norgen’s proprietary resin. Second, the kit contains A.

niger Master Mix and controls to allow for PCR

amplification. The amplified PCR products are then

detected using agarose gel electrophoresis. Alternatively,

detection can be performed based on real-time PCR

using melt curves.

The A. niger Mastermix contains reagents and enzymes for

the specific amplification of a 363 bp region of the fungal

genome. In addition, Norgen’s A. niger PCR Detection Kit

contains a second Mastermix, the PCR Control Master

Mix, which can be used to identify possible PCR inhibition

and/or inadequate isolation via a separate PCR reaction

with the use of the provided PCR control (PCRC) or

Isolation Control (IsoC), respectively. This kit is designed to

allow for the testing of 24 samples. The Aspergillus

niger PCR Primer Set and Controls are also available

separately for end-point PCR detection.

Features and Benefits

Rapid isolation of high quality DNA from plants

Contains two ready-to-use 2X PCR Master Mixes


Includes an isolation control and a PCR control


Ideal for use in:


2. Surveys

Linear Range

The linear range of Norgen’s Aspergillus niger PCR

Detection Kit was determined by analyzing a dilution

series of an A. niger quantification standards ranging

from 1 x 106 cfu/µL to 1 x 101 cfu/µL.

Each dilution has been tested in replicates (n = 4)

using Norgen’s Aspergillus niger PCR Detection Kit on

a 1X TAE 1.7% agarose gel.

The linear range of Norgen’s Aspergillus niger PCR

Detection Kit has been determined to cover

concentrations from 600 fg to 6 ng

Under the conditions of the Norgen’s Aspergillus

niger DNA Isolation procedure, Norgen’s Aspergillus

niger PCR Detection Kit covers a linear range from

100 copies to 1 x 105 copies/mg of plant tissue

For research use only and NOT intended for in vitro diagnostics

Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com

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Aspergillus niger PCR Detection Kit Cat. # 32900

Description Cat # Size

End-Point PCR 32900 24 rxns

Real-Time PCR TM32900 48 rxns

Real-Time PCR SG32900 48 rxns

Primer Sets and Controls 32910 100 rxns

Aspergillus niger Ordering information

Figure 1. Isolation and Detection of DNA from Fungi Samples with

No PCR Inhibition. Total genomic DNA was isolated from 40 mg

(wet weight) each of 8 economically important fungi species

affecting the grape industry using Norgen’s Fungi/Yeast

Genomic DNA Isolation Kit, which uses the same DNA isolation

technology as the Botytis cinerea PCR Detection Kit. The isolated

DNA was then subjected to quantitative PCR using universal

Intergenic Transcribed Space (ITS) gene primers to detect the

genomic DNA using the iQ SYBR Green Supermix (BioRad, #170-

8882). The black line below the PCR baseline is corresponding to

no template control while all other colored lines above the PCR

baseline are corresponding to the DNA isolated from 8 different

fungi species. The genomic DNA could be detected in all fungi

samples, indicating the high quality of the purified DNA.



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detection of B. cinerea using end-point PCR

Norgen’s Botrytis cinerea PCR Detection Kit is a ready-to-

use system for the isolation and detection of B.

cinerea from plant samples. First, the kit contains

components for the rapid isolation of total DNA, including

fungal DNA, from plant samples using spin-column

chromatography. Second, the kit contains B.

cinereaMaster Mix and controls to allow for PCR




using melt curves.

The B. cinerea Master Mix contains reagents and enzymes

for the specific amplification of a 381 bp region of the

fungal genome. In addition, Norgen’s B. cinerea PCR

Detection Kit contains a second Mastermix, the PCR

Control Master Mix, which can be used to identify possible

PCR inhibition and/or inadequate isolation via a separate

PCR reaction with the use of the PCR control (PCRC) or

Isolation Control (IsoC), respectively. This kit is designed to

allow for the testing of 24 samples. The Botrytis

cinerea PCR Primer Set and Controls are also available








Ideal for use in:


2. Surveys

Linear Range

The linear range of Norgen’s Botrytis cinerea PCR


series of a B. cinerea quantification standards ranging

from 100 ag to 1 pg


using Norgen’s Botrytis cinerea PCR Detection Kit on a

1X TAE 1.7% agarose gel.

The linear range of Norgen’s Botrytis cinerea PCR


concentrations from 100 ag to 1 ng

Under the conditions of the Norgen’s Botrytis

cinerea DNA Isolation procedure, Norgen’s Botrytis

cinerea PCR Detection Kit covers a linear range from

100 copies to 1 x 106 copies.

Botrytis cinerea PCR Detection Kit Cat. # 29400






Botrytis cinerea Ordering information

Figure 1: Detection of C. cladosporioides using the C.

cladosporioides PCR Detection Kit. A representative 1X TAE 1.5%

agarose gel showing the amplification of serially diluted C.

cladosporioides positive (lane 1 to 4) negative (lane NTC)

controls. The size of the C. cladosporioides target amplicon


Marker (M).


detection of C.cladosporioides using end-point PCR

Norgen’s Cladosporium cladosporiodes PCR Detection Kit

is a ready-to-use system for the isolation and detection of

C. cladosporiodes from plant samples. First, the kit

contains components for the rapid isolation of total DNA,

including fungal DNA from plant samples using spin-

column chromatography based on Norgen’s proprietary

resin. Second, the kit contains C. cladosporiodes Master

Mix and controls to allow for PCR amplification, as well as

a Control Master Mix to allow for amplification of both an

Isolation Control and a PCR Control. The amplified PCR

products are then detected using agarose gel

electrophoresis. Alternatively, detection can be

performed based on real-time PCR using melt curves.

The C. cladosporioides Master Mix contains reagents and

enzymes for the specific amplification of a 320 bp region

o f t h e f u n g a l g e n o m e . I n a d d i t i o n ,

Norgen’s Cladosporium cladosporiodes PCR Detection Kit

contains a second Master Mix, the Control 2X PCR Master


and/or inadequate isolation via a separate PCR with the

use of the provided PCR Control (PCRC) or Isolation

Control (IsoC). This kit is designed to allow for the testing of

24 samples. The Cladosporium cladosporiodes PCR Primer

Set and Controls are also available separately for end-

point PCR detection.







Ideal for use in:


2. Surveys

Linear Range

The linear range was determined by analyzing a

dilution series of a C. cladosporioides quantification

standards ranging from 1 x 106 cfu/µL to 1 x 101 cfu/µL.


using on a 1X TAE 1.7% agarose gel.

The linear range of Norgen’s Cladosporium

cladosporioides PCR Detection Kit has been

determined to cover concentrations from 7 pg to 70

ng

Under the conditions of the

Norgen’s C.cladosporioides DNA Isolation procedure,

Norgen’s Cladosporium cladosporioides PCR

Detection Kit covers a linear range from 1500 copies

to 1.5 x 106 copies/50 mg of plant tissue



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Cladosporium cladosporioides PCR Detection Kit Cat. # 33000






C. cladosporioides Ordering information

(320 bp)

Figure 1. Sensitivity of Detection using the Penicillium sp. PCR

Detection Kit. A representative 1X TAE, 1.7% agarose gel showing

the amplification of Penicillium sp. at different concentrations

(Target). The size of the Penicillium sp. target amplicon

corresponds to the 327 bp band represented by the provided

DNA Marker (M). The size of the Isolation Control corresponds to

the 550 bp band represented by the provided DNA Marker (M).

The Penicillium sp. 2X PCR Master Mix contains a PCR Control

which controls for PCR inhibition. The size of the PCR Control

corresponds to the 171 bp band represented by the provided

DNA Marker (M). Lanes A-E represents samples spiked with

different Penicillium sp. concentrations isolated from 50 mg plant

samples (interpreted as positive results). Lane F represents a no

fungus control.

Figure 2. Specificity of the Primers used to Detect Penicillium sp. A

representative 1X TAE, 1.7% agarose gel showing the specificity

of the primers used to detect the Penicillium sp. The size of

the Penicillium sp. target amplicon corresponds to the 327 bp

band represented by the provided DNA Marker (M). The

specificity of Norgen’s Penicillium sp. PCR Detection Kit is first and

foremost ensured by the selection of the Penicillium sp. specific

primers, as well as the selection of stringent reaction conditions.

The primers were checked for possible homologies in GenBank

published sequences by sequence comparison analyses.

Furthermore, the specificity of the Penicillium sp. specific primers

were tested against most of common fungi pathogens. Lane A

represents the gDNA from Aspergillus niger, Lane B represents the

gDNA from Cladosporium cladosporioides, Lane C represents the

gDNA from Botrytis cinerea, Lane D represents the gDNA

from Mucor racemosus, Lane E represents the gDNA

from Alternaria tenuissima, Lane F represents the gDNA

from Penicillin sp., Lane G represents the gDNA from Rhizopus

oryzae and Lane H represents the gDNA from Fusarium

oxysporum.



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detection of Penicillium sp. using end-point PCR

Norgen’s Penicillium sp. PCR Detection Kit is a ready-to-

use system for the isolation and detection of Penicillium

sp from plant samples. First, the kit contains components

for the rapid isolation of total DNA, including fungal DNA,

from plant samples using spin-column chromatography

based on Norgen’s proprietary resin. Second, the kit

contains Penicillium sp. Master Mix and controls to allow

for PCR amplification. The amplified PCR products are

then detected using agarose gel electrophoresis.

Alternatively, detection can be performed based on real-

time PCR using melt curves.

The Penicillium sp. Master Mix contains reagents and


of the fungal genome. In addition, Norgen’sPenicillium sp.

PCR Detection Kit contains a second heterologous

amplification system to identify possible PCR inhibition

and/or inadequate isolation. This kit is designed to allow

for the testing of 24 samples. The Penicillium sp PCR Primer









Ideal for use in:


2. Surveys

Linear Range

The linear range of Norgen’s Penicillium sp.PCR


series of a Penicillium sp.quantification standards

ranging from 1 x 106 cfu/µL to 1 x 101 cfu/µL.


using Norgen’s Penicillium sp. PCR Detection Kit on a

1X TAE 1.7% agarose gel.

The linear range of Norgen’s Penicillium sp.PCR


concentrations from 18 pg to 18 ng

Under the conditions of the Norgen’s Penicillium

sp.DNA Isolation procedure, Norgen’s Penicillium

sp.PCR Detection Kit covers a linear range from 640

copies to 6.4 x 105 copies.

Penicillium sp. PCR Detection Kit Cat. # 33200






Penicillium sp. Ordering information

(327 bp)

Figure 1. Isolation and Detection of DNA from Yeast Samples with

No PCR Inhibition. Total genomic DNA was isolated from 500 µL

cultures of 4 economically important yeast species affecting the

grape industry using Norgen's Fungi/Yeast Genomic DNA

Isolation Kit, which uses the same DNA isolation technology as

the S. cerevisiae PCR Detection Kit. The isolated DNA was then

subjected to quantitative PCR using universal Intergenic

Transcribed Space (ITS) gene primers to detect the genomic DNA

using the iQ SYBR Green Supermix (BioRad, #170-8882). The black

line below the PCR baseline is corresponding to no template

control while all blue lines above the PCR baseline are

corresponding to the DNA isolated from 4 different yeast species.

The genomic DNA could be detected in all yeast samples,

indicating the high quality of the purified DNA.

Figure 2. Detection of S. cerevisiae using the S. cerevisiae PCR

Detection Kit. A representative 1X TAE 1.5% agarose gel showing

the amplification of S. cerevisiae positive (lane 1 and 2) negative

(lane 3 and 4) controls. The size of the S. cerevisiae target

amplicon corresponds to 301 bp as represented by the provided

DNA Marker (M).


detection of S. cerevisiae using end-point PCR

Norgen’s Saccharomyces cerevisiae PCR Detection Kit is

a ready-to-use system for the isolation and detection of S.

cerevisiae from cultures, plants, and beverage samples.

First, the kit contains components for the rapid isolation of

total DNA, including fungal DNA, from thesamples using

spin-column chromatography based on Norgen’s

proprietary resin. Second, the kit contains S.

cerevisiae Master Mix and controls to allow for PCR




using melt curves.

The S. cerevisiae Master Mix contains reagents and


o f t h e f u n g a l g e n o m e . I n a d d i t i o n ,

Norgen’sSaccharomyces cerevisiae PCR Detection Kit

contains a second Mastermix, the PCR Control Master


and/or inadequate isolation via the internal PCR control

(PCRC) or the provided Isolation Control (IsoC),

respectively. This kit is designed to allow for the testing of

24 samples. The Saccharomyces cerevisiae PCR Primer









Ideal for use in:


2. Surveys

Linear Range

The linear range of Norgen’s S. cerevisiae PCR


series of a S. cerevisiae quantification standards

ranging from 1 x 106 cfu/µL to 1 x 101 cfu/µL.


using Norgen’s S. cerevisiaePCR Detection Kit on a 1X

TAE 1.7% agarose gel.

The linear range of Norgen’s S. cerevisiae PCR


concentrations from 110 pg to 11 ng

Under the conditions of the Norgen’s S.

cerevisiae DNA Isolation procedure, Norgen’s S.

cerevisiae PCR Detection Kit covers a linear range

from 800 copies to 8.5 x 105 copies.



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Saccharomyces cerevisiae PCR Detection Kit Cat. # 33300






Saccharomyces cerevisiae Ordering information

Figure 1. Detection using the Plum Pox Virus RT-PCR Detection

Kit. A representative 1X TAE 1.5% agarose gel showing the

amplification of PPV negative (lanes 6 and 7) and PPV positive

(lanes 1 to 5) controls. The size of the PPV target amplicon


Marker (M).



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detection of Plum Pox Virus using end-point PCR

Norgen’s Plum Pox Virus RT-PCR Detection Kit is a ready-to

-use system for the isolation and detection of Plum Pox

Virus from plant samples. First, the kit contains

components for the rapid isolation of total RNA, including

viral RNA, from plant samples using spin-column

chromatography based on Norgen’s proprietary resin.

Second, the kit contains Plum Pox Virus Master Mix and

controls to allow for PCR amplification, as well as a

Control Master Mix to allow for amplification of both an

isolation control and a PCR control. The amplified PCR

products are then detected using agarose gel



The Plum Pox Virus Master Mix contains reagents and


of the viral genome. In addition, Norgen’s Plum Pox Virus

RT-PCR Detection Kit contains a second Mastermix, the

Control 2X RT-PCR Master Mix, which can be used to

identify possible PCR inhibition and/or inadequate

isolation via a separate RT-PCR reaction with the use of

the provided PCR control (PCRC) or Isolation Control

(IsoC), respectively. This kit is designed to allow for the

testing of 24 samples. The Plum Pox Virus RT-PCR Detection

Kit is also available in a 96-well format for high throughput

analysis. The Plum Pox Virus RT-PCR Primer Set and

Controls Controls are also available separately for end-

point PCR detection,







Ideal for use in:


2. Surveys

Linear Range

The linear range of Norgen’s PPV RT-PCR Detection Kit

was determined by analyzing a dilution series of an

PPV quantification standards ranging from 100 ag to 1

pg.


using Norgen’s PPV RT-PCR Detection Kit on a 1X TAE

1.5% agarose gel.

The linear range of Norgen’s PPV RT-PCR Detection Kit

has been determined to cover concentrations from

100 ag to 1 ng

Under the conditions of the Norgen’s PPV RNA

Isolation procedure, Norgen’s PPV RT-PCR Detection

Kit covers a linear range from 100 copies to 1 x 106

copies.

Plum Pox Virus PCR Detection Kit Cat. # 33400






Plum Pox Virus Ordering information

(295 bp)

Figure 1. Sensitivity of Detection using the Phomopsis viticola PCR

Detection Kit. A representative 1X TAE, 1.7% agarose gel showing

the amplification of P. viticola at different concentrations

(Target). The size of the P. viticola target amplicon corresponds to

the 300 bp band represented by the provided DNA Marker (M).

The size of the Isolation Control corresponds to the 529 bp band

represented by the provided DNA Marker (M). The P. viticola 2X

PCR Master Mix contains a PCR Control which controls for PCR

inhibition. The size of the PCR Control corresponds to the 171 bp

band represented by the provided DNA Marker (M). Lanes A-D

represents samples spiked with different P. viticola concentrations

isolated from 50 mg plant samples (interpreted as positive

results). Lane E represents a no fungus and Isolation control.

Figure 2. Specificity of the Primers used to Detect P. viticola. A

representative 1X TAE, 1.7% agarose gel showing the specificity

of the primers used to detect the P. viticola. The size of the P.

viticola target amplicon corresponds to the 300 bp band

represented by the provided DNA Marker (M). The specificity of

Norgen's P. viticola PCR Detection Kit is first and foremost ensured

by the selection of the P. viticola specific primers, as well as the

selection of stringent reaction conditions. The primers were

checked for possible homologies in GenBank published

sequences by sequence comparison analyses. Furthermore, the

specificity of the P. viticolaspecific primers were tested against

most of common fungi pathogens. Lane A represents the gDNA

from Aspergillus niger, Lane B represents the gDNA from

Cladosporium cladosporioides, Lane C represents the gDNA

from Botrytis cinerea, Lane D represents the gDNA from Mucor

racemosus, Lane E represents the gDNA from Alternaria

tenuissima, Lane F represents the gDNA from Penicillium sp., Lane

G represents the gDNA from Rhizopus oryzae, Lane H represents

the gDNA from Fusarium oxysporum and Lane I represents the

gDNA from P. viticola.


detection of P. viticola using end-point PCR

Norgen’s Phomopsis viticola PCR Detection Kit is a ready-

to-use system for the isolation and detection of Phomopsis

viticola from plant samples. First, the kit contains


fungal DNA, from plant samples using spin-column

chromatography based on Norgen’s proprietary resin.

Second, the kit contains Phomopsis viticola Master Mix

and controls to allow for PCR amplification. The amplified

PCR products are then detected using agarose gel



The Phomopsis viticola. Master Mix contains reagents and


of the fungal genome. In addition, Norgen’s Phomopsis

viticola PCR Detection Kit contains a second

heterologous amplification system to identify possible PCR

inhibition and/or inadequate isolation. This kit is designed

to allow for the testing of 24 samples. ThePhomopsis

viticola PCR Primer Set and Controls are also available








Ideal for use in:


2. Surveys

Linear Range

The linear range (analytical measurement) of

Norgen’s Phomopsis viticola PCR Detection Kit was

determined by analyzing a dilution series of a P.

viticolaquantification standards ranging from 1 x

107 cfu/µL to 1 x 103 cfu/µL.


using Norgen’s Phomopsis viticola PCR Detection Kit

on a 1X TAE 1.7% agarose gel.

The linear range of Norgen’s Phomopsis viticola PCR


concentrations of genomic DNA from 10 pg to 100 ng

Under the conditions of the Norgen’s Phomopsis

viticola DNA Isolation procedure, Norgen’s Phomopsis

viticola PCR Detection Kit covers a linear range from

500 copies to 5 x 106 copies.



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Phomopsis viticola PCR Detection Kit Cat. # 34900






Phomopsis viticola Ordering information

(300 bp)

Figure 1. Sensitivity of Detection using the Erwinia amylovora PCR

Detection Kit. A representative 1X TAE 1.5% agarose gel showing

the amplification of Erwinia amylovora at different

concentrations. The size of theE. amylovora target amplicon


Marker (M). NTC = Negative Control.



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detection of E. amylovora using end-point PCR

Norgen’s Erwinia amylovora PCR Detection Kit is a ready-

to-use system for the isolation and detection of Erwinia

amylovora from plant samples. First, the kit contains


bacterial DNA, from bacterial cultures and plant samples

using spin-column chromatography based on Norgen’s

proprietary resin. Second, the kit contains Erwinia

amylovora Master Mix and controls to allow for PCR




using melt curves.

The Erwinia amylovora Master Mix contains reagents and


of the bacterial genome. In addition, Norgen’s Erwinia

amylovora PCR Detection Kit contains a second

heterologous amplification system to identify possible PCR

inhibition and/or inadequate isolation. This kit is designed

to allow for the testing of 24 samples. The Erwinia

amylovora PCR Primer Set and Controls are also available








Ideal for use in:


2. Surveys

Linear Range

The linear range (analytical measurement) of

Norgen’s Erwinia amylovora PCR Detection Kit was

determined by analyzing a dilution series of E.

amylovora quantification standards ranging from 1 x

106 cfu/µL to 1 x 102 cfu/µL.


using Norgen’s Erwinia amylovora PCR Detection Kit

on a 1X TAE 1.7% agarose gel.

The linear range of Norgen’s Erwinia amylovora PCR


concentrations of genomic DNA from 1 fg to 1 ng

Under the conditions of the Norgen’s Erwinia

amylovora DNA Isolation procedure, Norgen’s Erwinia

amylovora PCR Detection Kit covers a linear range

from 30 copies to 3 x 106 copies.

Erwinia amylovora PCR Detection Kit Cat. # 35100






Erwinia amylovora Ordering information

3430 Schmon Parkway, Thorold, ON L2V 4Y6 Canada

Phone: (905) 227-8848

Toll Free: 1-866-NORGENB (667-4362)

Fax: (905) 227-1061

Email: [email protected]

Commitment to Quality

www.norgenbiotek.com An ISO 13485:2003, ISO 9001:2008 & ISO 15189:2012 Certified Company

mailto:[email protected]

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