plant dna digests - michigan state university plant dna digests plant plasmid css451 digests plant...
TRANSCRIPT
![Page 1: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/1.jpg)
Plant DNA digests
![Page 2: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/2.jpg)
Plasmid DNA digests Plasmid DNA digests
Plant DNA digests CSS451 Plant DNA digests
![Page 3: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/3.jpg)
Plant Plasmid
CSS451 digests
![Page 4: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/4.jpg)
Plant Plasmid
![Page 5: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/5.jpg)
Southern AnalysisOptimizing DNA digest concentrations
2 4 8 12 16 20 24 ug
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Estimating Yield of Dig-labeled Probe
1:5 1:50 1:500 1:5000 1:50,000
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Plasmid DNA Southern Analysis
Bt-cry52244 bp
![Page 9: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/9.jpg)
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2.0kb1.9kb
21kb
5.1kb4.9kb
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M NT LM L235 C3 80-1
Southern Analysis
Copynumber
Integration
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NT LM L23 C3 80-11 2 4 5 6 7 8 9 10 11 12 13 14 15
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Northern Analysis
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Southern Analysis
Non-specificBinding of probebecause wrong buffer was used.
Non-bindingOf Probebecause of poorHybridization.
Image of Whole membrane
Dig-labeled size marker
![Page 13: Plant DNA digests - Michigan State University Plant DNA digests Plant Plasmid CSS451 digests Plant Plasmid Southern Analysis Optimizing DNA digest concentrations 2 4 8 12 16 20 24](https://reader031.vdocuments.site/reader031/viewer/2022022006/5abe8e3b7f8b9ad8278d4f7e/html5/thumbnails/13.jpg)
Southern Analysis:Light-exposed film
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Southern Analysis:Light-exposed film
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cryV-BtPVYcp
RAPD Analysis of Bt-cry5/PVYcp transgenic potatoes
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Southern Analysis of Bt-cry5/PVYcp transgenic potatoes:Integration
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4.9kb5.1kb
Southern Analysis of Bt-cry5/PVYcp transgenic potatoes:Copy Number
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Northern Analysis of Bt-cry5/PVYcp transgenic potatoes:Leaf tissue
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1.2kb
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Northern Analysis of Bt-cry5/PVYcp transgenic potatoes:Tuber tissue
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125kD
79.6kD
1 2 3 4 5 6 7 8 9 10 11 12 13
CRYV
Western Analysis of Bt-cry5 Potatoes
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1 2 3 4 5 6 7 8 9 10 11 12 13 14 1516
500 bp
456 bp
256 bp
PVY
Cry1a
NPTII
Progeny analysis of crosses between PVYcp and Bt-transgenic potatoes:
PCR results
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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1621kb
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PVY
Cry1a
Progeny analysis of crosses between PVYcp and Bt-transgenic potatoes:
Southern Analysis results
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Fingerprint Analysis of Potato Varieties