Enhancing Agricultural Productivity through Application of Genomics Technologies

ICAR-CII Meet 2011Ajit NottDuPont Agricultural Biotechnology

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Products- Corn- Soybeans- Canola- Sunflower- Rice- Sorghum- Alfalfa- Wheat- Pearl Millet- Mustard- Cotton

End Use Segments- White - Waxy- Wet Milling- Dry Grind Ethanol- Food Corn- Soy Oils /Meals

Founded By Henry Wallace as the Hi-Bred Seed Company in 1926 – the first company to develop, produce and market hybrid seed corn

Pioneer Hi-Bred: a world leader in seed genetics

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Agricultural Biotechnology at the DuPont Knowledge Center, Hyderabad

A comprehensive science and technology center that

• discovers yield, drought tolerance and improved nitrogen-use efficiency traits using high throughput genetic screens and advanced computational methods,

• characterizes and develops rice traits for Indian and SE Asian markets, and

• becomes a global center for regulatory science research

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GENE DISCOVERY HTP FUNCTIONAL SCREENS

POSITIONAL CLONING GENE OPTIMIZATION

TRANSGENIC PLANT DEVELOPMENT

PLANT TISSUE CULTURE TRAIT OPTIMIZATION

NEW VARIETIESYIELD TRIALS

PRODUCT TESTING

GENETIC VARIATION PRECISION PHENOTYPINGGENETIC ASSOCIATIONS

GERMPLASM DEVELOPMENT

MOLECULAR BREEDINGDOUBLED HAPLOIDS

GENETIC KNOWLEDGE

ENABLING TECHNOLOGY PLATFORMS

PRODUCT OPPORTUNITY BY TRAIT, CROP

Complementary technologies for trait discovery and product development

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Native tolerance and AYT™ breeding technology • Targeted drought screening• Whole genome genetic

prediction

Target market: dry land and limited irrigation acres

Superior germplasm• Selected for drought

tolerance• Utilizing global genetic

diversity• Industry-leading drought

tolerance genetic pool

Transgenic and native trait approaches• Robust pipeline of proprietary

leads• Surrogate drought assays• FAST corn technology

Broad acreage applications

Industry-leading

HYBRIDSPower of genetics through

NATIVE TRAITSNext generation

TRANSGENIC TRAITS

35F44

Herculex® XTRA Insect Protection technology by Dow AgroSciences and Pioneer Hi-Bred. ® Herculex and the HX logo are registered trademarks of Dow AgroSciences LLC. LibertyLink ® is a registered trademark of Bayer. ® Roundup Ready is a registered trademark used under license from Monsanto Company. Products will not be offered for sale or distribution until completion of field testing and approval by regulatory authorities. ©2011 PHII.

Performance leadership in water-limited environments through an integrated approach

DROUGHT TOLERANCE in Maize

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Proprietary Pioneer Technology to increase breeding accuracy and efficiency

The process• Seeds are oriented with magnetic

paint for precise laser slicing

• Sample collected for DNA analysis while maintaining seed viability

• Seeds are transferred for storage and planting

Benefits• Increase breeding accuracy• Increase breeding efficiency

©2011 PHII

LASER-ASSISTED SEED SELECTION

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Discovering and developing the next generation crops requires multiple competencies

Technology intensive trait and breeding programs - Broad-based access to plant and microbial biodiversity

- Capability to identify or generate novel traits

- Wide area testing in multiple climatic zones and against evolving and diverse pests and weeds

Intellectual property strategies for trait and technology protection and for freedom-to-operate

Regulatory science and registration competencies across non-harmonized national systems

Consumer awareness and acceptance for GM traits

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• Any statements made regarding regulatory approval and commercial distribution of new products are forward-looking statements based on current information and are subject to risks and uncertainties including, but not limited to, when and if such regulatory approval will occur, any conditions that may be imposed on such approval, costs and time of additional data that may be required in the approval process or changes in government laws or policies. Statements included herein are made as of the date hereof, and Pioneer Hi-Bred undertakes no obligation to update such statements to reflect subsequent events or circumstances. Products will not be offered for sale or distribution until completion of field testing and approval by regulatory authorities.