phytochemical study and in vitro biological assays on
TRANSCRIPT
Phytochemical study and in vitro biological assays on Zingiber officinal: a widely used spice
Toma Nardjes Mouas 1,*, Zahia Kabouche 1, Amine Boucherka 2, and Abdenour Messaoud 2
1 Université frères Mentouri-Constantine1, Laboratoire d'Obtention de SubstancesThérapeutiques LOST, Campus Chasbet Ersas, 25000 Constantine, Algeria;2 Constantine 25000, Algeria.•Corresponding author: [email protected]
Laboratoire d’Obtentionde Substances Thérapeutiques 1
Abstract: Zingiber officinal is a widely used plant in cooking as well astraditional remedy in prevention of digestive conditions anddisorders, cancer, inflammatory disease, antiseptic. In recent years severalstudies are conducted on botanical, chemical and toxicological parts of thisplant in order to prove a concordance between traditional and medicinalknowledge. There is an urgent need to explore and investigate theinnovations, current shortcomings, future challenges explore and conveythe key concepts for understanding the assessment of plant basedmetabolites in therapeutically caring. Furthermore, Drug discovery fromplants goes through different strategies: empirical approach like ethnobotanical and pharmalogical studies, and chimiotaxonomical one likechoosing certain secondary metabolites family phenols, flavones, terpens...In this case, the present work is a contribution in the evaluation of Zingiberofficinal's rhizome percolate potential in polyphenols, flavonoïdes, in vitroantioxidant test Ferric reducing antioxidant power FRAP, antibacterialactivity against several gram + (S.aureus) and gram-( E.coli, P.aeruginonose, K.pneumoniae) referential strains and antifungal activity(Candida albicans, Aspergillus niger,) were tested using disk diffusionmethod, which reveled a very interesting dose-depending activity (from 16to 26mm) against S.aureus, P.aeruginonose and K.pneumoniae, similar to usedstandard Gentamicin GN, whereas fungi exhibit less sensitivity with 10mmof inhibition and E.coliwas resistant to crud ginger extract.Keywords: Zingeber officinal, phenols, antioxidant activity, antimicrobial activity. 2
Zingebre Officinale
Origin Asie du Sud-est (
Indonésie et Philippines)
Chine Inde Afrique tropicale
(Nigéria)
Classification
Famille Zingibéracées
Genre Zingiber Mill.
Espèce Zingiber
officinale
Uses Epice. Remède contre les
douleurs musculaires, les troubles gastro-intestinal, les inflammations des voies respiratoires…etc
Composition biochimique du Zingebre Officinale
Métabolites primaires
60% glucides
10% lipides
Protéine, fibres, minéraux, vitamines… etc
Métabolites secondaires
Polyphénols
Flavonoïdes
Terpènes… etc.
H
Zingebrene(31%)
Ar-curcumene(15,4%)
B-sesquiphellandrene(14,02%)
Z.Officinale
Broyage
Extraction par Soxhlet
Evaporisationpar Rotavapor
Crud extract
Yield= 19,06 %
Results and Discussion
PHYTOCHEMISTRY
Qualitative
TLC
Hexane/Acetate d’éthyle 21/9
Hexane/Acetate d’éthyle 24/6
Hexane/Acetate d’éthyle 27/3
Quantitative
Dosage des polyphénols et des flavonoides
0
2
4
6
8
10
12
14
16
18
16.84
1.78
3.56
11.5125
La t
en
eu
r e
n f
lavo
no
ïde
s e
n m
g EA
G/g
Extration par Soxhlet
Extration par macératopn
Extration par hydro-distilation
Phenols
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
Extrait chloroformiqueExtrait méthanol / eau Huile essentielExtrait méthanolique (etudie)
1.56
0.02
5
2.1175
La t
en
eu
r e
n f
lavo
no
ïde
s e
n m
g/g
EQ
Extration par macératopn
Extration par hydro-distilation
Extration par Soxhlet
Flavonoids
Biological activities
Antioxidant
FRAP test
Antimicrobial
Diffusion discks
0
5
10
15
20
25
30
SM 1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128,
Dia
met
re d
es z
on
es d
'inh
ibit
ion
(m
m)
Concentration (mg/l)
S.aureus Escherichia coli P.aeruginonose K.pneumoniae C.albican A.niger
Dose-dependending antimicrobial activity
0
5
10
15
20
25
30
35
40
S.aureus E.coli P.aeruginonose K.pneumoniae
Dia
mét
re d
es z
ones
d'in
hib
itio
n.
Extrait méthanolique étudier GN
Conclusions In the present work a widely used spice inculinary field as well as traditional remedy Zingeberofficinal rhizomes'' methanol percolate was investigatedthrough its in vitro antioxidant and antimicrobial activitiesassessment. Phenols potent was evaluated. The antioxidanteffect of studied plant was evaluated by the FRAP testwhich gave high effect at low concentration similar tovitamin C, the antimicrobial activity was evaluated by diskdiffusion method for three Gram (-) bacteria(E.coli, P.aeruginonose, K.pneumoniae), one bacteria Gram (+)(Staphylococcus aureus) and two funguses: Candidaalbicans, Aspergillus niger. and exhibit an interestingantibacterial effect for P.aeruginonose, K.pneumoniaeandStaphylococcus aureus, similar to used standard GentamicinGN. Therefore, it may be considered, as an efficientnutraceutical and functional food for treatinggastrointestinal disorder.
AcknowledgmentsAuthors would like to thank Algerian Ministry of Higher Education and Scientific Research DGEFS, and the Algerian Directorate General for Scientific Research and Technological Development DGRSDT for financial fund.
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