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  • Phyto66n07 924 - APS Home...pesticide-quality (spectro-grade) benzene using a Waring Blendor. Samples were evaporated to dryness in a flash evaporator at 55 C. The residues were resuspended
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Phyto66n07_924.pdf

Bacillus Calmette-Guérin Suppresses Asthmatic Responses ... · supernatant, the pellet was resuspended in 100 µL PBS. Total BAL cell counts were performed using a haemacytometer

Bacillaene and Sporulation Protect Bacillus subtilis from ... · M. xanthus cells were resuspended in MMC buffer to a final concentration of 2 109 cells/ml. Prey cells were grown

til) 10Cc/) · ml; pH6.8. Plasmolyzed cells were fixed in 10Cc/) formalin for 1 hr at 24 C. The cells were then centrifuged, and the pellet was resuspended in 1 ml of 1 % buffered

Supporting Information - Proceedings of the National ... · PDF filecells were spread on LB agar plates containing 100 μgmL−1 ... washed once with 0.9% NaCl solution. ... resuspended

In vivo and in vitro characterization of the intestine of ... 623.pdf · tilled H O. Worms were resuspended in 45 111 water and 5 of 37 % formaldehyde was added for 6 h. Worms were

Supporting Information - PNAS · Supporting Information Yao et al. 10.1073/pnas.1109029108 SI Materials and Methods Flow Cytometry. For flow cytometry, cells were resuspended in

miR-1298 Inhibits Mutant KRAS-Driven Tumor Growth by ... · NSCLC cells were infected with miR-Ctrl- or miR-1298– expressing lentiviruses for 72hours washed, and resuspended. Groups

Supporting Information - PNAS · for 10 min to remove unbroken cells. The supernatant was centri-fuged at 100,000 × g for 1 h to pellet membranes. Resuspended membranes were solubilized

The Journal of Immunology Md.). The cells from these sources were resuspended in RPMI 1640 media (GIBCO, Grand Island, N. Y. ) containing heat-inactivated fetal calf serum (Reheis

Supplemental Materials · collagenase type II (Worthington Biochemicals, CLS-2) and 60 U/mL DNase1 (AppliChem, A3778.0500). Cells were washed and resuspended in cold PBS supplemented

Flow Cytometry Sorting of Recombinant … › content › cdli › 18 › 1 › 43.full.pdfStained mycobacteria were washed twice with 0.5 ml PBS and resuspended in 100 l PBS, and

Home: Cell Press€¦ · Web viewThe transformed Agrobacterium EHA105 cells were collected and resuspended in liquid AAM-AS medium (OD600=0.1) containing 100μM acetosyringone. After

Corning® Matrigel® Matrix-3D Plates for High Throughput 3D ... · While plates were polymerizing, cells were harvested with Accutase (Corning 25-058-CI) and resuspended at a density

Spatial and Temporal Deposition of Adhesive Extracellular ... · Cultures (100 ml) of MHS-3 in the early stationary phase were pelleted by centrifugation (10,000 3 g), resuspended

SecondaryStorageofDermatanSulfateinSanfilippo Disease · resuspended in 2 ml of 20 mM HEPES buffer, pH 8.2, and lysed by brief sonication. Lysates were digested with 1 mg/ml bovine

Biogenesis of Chloroplast Membranes · 4298 Biogenesis of Chloroplast Membranes. VI Vol. 245, iSo. 17 cells were resuspended in fresh growth medium with the addition of 2 mM KH2P04

A novel population of extracellular vesicles smaller than … · 2019. 8. 15. · volume of media were resuspended in 60μLPBS.SNΔ2 was concentrated in Amicon® Ultra-4 10kDa nominal

Determination of Genome Size, Macrorestriction Pattern ... · 2080 LUCIER ANDBRUBAKER which cells were resuspended to an OD620 of 10 so that enough DNAwould be present for visualization

ResearchAssessing agonistic potential of a candidate ... · counts were measured using a Pentra 60C (Horiba ABX Diagnostics, Irving, CA). Cells were resuspended in RPMI with complete

Chapter 2 Feb 16, 2005 - MIT OpenCourseWare · 1) for chemotaxis or Matrigel (28 µg per insert) and fibronectin for invasion studies. Breast cancer cells were resuspended in chemotaxis

Induced pluripotent stem cells offer new approach to ... · -thalassemia iPS colonies with the enzyme Accutase. After washing, the cells were resuspended in the first differentiation

Human Papillomavirus Genotype-Specific Prevalence Across the … · 2014. 11. 17. · Without removal of paraffin wax, the tissue sections obtained for HPV genotyping were resuspended

Isolation and Characterization of the Erythroid Progenitor ... · Isolation and Characterization of the Erythroid Progenitor Cell" ... pellets were resuspended in 0.5 ml of a-medium

Next Generation CSox-based Substrate Sensors for ... · The Sox-based peptide substrates (AssayQuant Technologies, Marlborough, MA) were resuspended to create a 1 mM (100x) stock

YAP activation promotes the transdifferentiation of …...MUSE cell analyzer to assess cell cycle Neonatal rat cardiac fibroblasts were resuspended in PBS and added in drops into

May Biochemistry Rollingreplication ofshort DNA4642 Biochemistry: Fire andXu (1:1) and chloroform and precipitated twice with ethanol. Final samples were resuspended in 25,1 of TEN(10

Home | Cancer Immunology Research · Web viewT2 cells were washed in serum-free RPMI 1640 medium (Life Technologies, Inc., Carlsbad, CA), resuspended at a concentration of 2.5×106

Nucleologenesis in the Caenorhabditis elegansEmbryoImmunoelectron Microscopy Embryos were collected as described above. After three washes in M9, embryos were resuspended in 50 ml

Molecular Requirements for Self-Interaction of the ... · 1 mL of PBS, before being resuspended with 290 L of fresh PBS. Cells were further resuspended ... 5 M PJK, Kleinblittersdorf,

25(4), 547–553 Research Article ... · with PBSM, 20µl of anti-rabbit IgG Microbeads (MiltenyiBiotec GmbH, Gladbach, Germany) and 80µl of resuspended cells were incubated at 4°C

Tissue Stiffness and Hypoxia Modulate the Integrin-Linked Kinase … · Approximately 5,000 cells were resuspended in serum-free DMEM/F12 medium supplemented with 0.35% agar and plated

AFRRI Reports - DTIC · min. The cells were resuspended in fresh TCM and returned to the incubator. All exposures and incuba- tions were performed with 2 ml cell suspensions in 15

Metabolic Crosstalk: Molecular Links Between Glycogen and ...vortexing and short incubation, samples were centrifuged at 13,000 rpm for 10 min. The bottom layer was dried and resuspended

NOD2-NitricOxide-responsiveMicroRNA-146aActivates ...Nuclear and Cytosolic Subcellular Fractionation—Macro-phages were harvested and gently resuspended in Buffer A (10 mM HEPES,

Ehrlich ascites tumour cells show tissue-specific ... · CHICK serum; me,n ui cenes were again conceniraie oy u centrifugation an resuspended d in the culture medium: EMEM supplemented