photon-timing detector module for single-molecule spectroscopy politecnico di milano i.rech*, m....
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PHOTON-TIMING DETECTOR MODULE PHOTON-TIMING DETECTOR MODULE FOR SINGLE-MOLECULE SPECTROSCOPYFOR SINGLE-MOLECULE SPECTROSCOPY
POLITECNICO DI MILANO
I.Rech*, M. Ghioni*, S. Cova*, H. Yang°, G. Luo°, X.S. Xie°
* Dip. di Elettronica e Informazione and IFN-CNR, POLITECNICO DI MILANO° Dep. of Chemistry and Chemical Biology, HARVARD UNIVERSITY
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Sommario :Sommario :
• Introduzione : perché misure su singole molecoleIntroduzione : perché misure su singole molecole
• Misure di fluorescenza “time-resolved”Misure di fluorescenza “time-resolved”
• Single Photon Avalanche Diode (SPAD)Single Photon Avalanche Diode (SPAD)
• Modulo SPTM2Modulo SPTM2
• Risultati sperimentaliRisultati sperimentali
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Perché misure su singole molecole?Perché misure su singole molecole?
1.1. Misure di proprietà di molecole Misure di proprietà di molecole NON MEDIATE SU INSIEMENON MEDIATE SU INSIEME
2.2. Possibilità di seguire Possibilità di seguire in tempo realein tempo reale l’evoluzione l’evoluzione
3.3. Informazioni sulla Informazioni sulla dinamicadinamica della molecola della molecola
Molecole di interesse biologico : ProteineMolecole di interesse biologico : Proteine
FRET : Fluorescence Resonance Energy Trasnfer
Variazioni di struttura nanometriche
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Single Molecule Structural DynamicsSingle Molecule Structural Dynamics
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Nuovo metodo di X.S. Xie, Nuovo metodo di X.S. Xie, Harvard UniversityHarvard University
- Basato su eccitazione con impulsi laser- Basato su eccitazione con impulsi laser
- Rivelazione fotoni fluorescenti e misure di:- Rivelazione fotoni fluorescenti e misure di:
t1 t2 t3
photon
Excitation pulse train
• Ritardo emissione risp. eccitazione• Tempo reale di emissione• Analisi per correlazione
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Setup di MisuraSetup di Misura
??
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Requisiti del RivelatoreRequisiti del Rivelatore
• Alta QE (Quantum detection Efficiency) > Alta QE (Quantum detection Efficiency) > 40%40%
• Basso rumore intrinsecoBasso rumore intrinseco < < 50c/s50c/s
• Alta Risoluzione Temporale (FWHM)Alta Risoluzione Temporale (FWHM) < < 100ps100ps
NON ESISTONO NON ESISTONO RIVELATORI COMMERCIALI RIVELATORI COMMERCIALI
CON QUESTE CARATTERISTICHECON QUESTE CARATTERISTICHE
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Single Photon Avalanche DiodeSingle Photon Avalanche Diode(SPAD)(SPAD)
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
PerkinElmer SPCM (SLIKPerkinElmer SPCM (SLIKTM TM diode)diode)
Reach-Trough structure
Typical active region:
200 m diameter
•QE: 30% (@500nm) OK
•Dark Counts: 150 c/s OK
•FWHM: >300ps NO!
• High voltage : 300 to 400V
• High dissipation
• Delicate and degradable
• Very expensive
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Sviluppo SPAD (Polimi e IMM-CNR)Sviluppo SPAD (Polimi e IMM-CNR)
Planar structure
typical active region:
20-50 m diameter
• Good QE and low noise
• Picosecond timing
• Low voltage : 15 to 40V
• Low power : cooling not necessary
• Standard Si substrate
• Planar fabrication process
• COMPATIBLE with array detector and integrated circuits
• Robust and rugged
• Low-cost
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Quantum Efficiency e Dark Counting RateQuantum Efficiency e Dark Counting Rate
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Modulo SPTM2Modulo SPTM2
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Modulo SPTM2Modulo SPTM2
Sfruttare le PerformanceSfruttare le Performancedel Rivelatoredel Rivelatore
SistemaSistemaCompatto e User FriendlyCompatto e User Friendly
• Compatto (82x60x30mm)
• Singola alimentazione
• Tensioni programmabili
• Hold off programmabile
• Interfaccia RS-232
• Controllo di Temperatura
• Pulse Counter integrato
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Avalanche PhotoDiode
APD
Linear-mode: AMPLIFICATORE
Single-Photon Avalanche Diode
SPAD
APD SPAD
Geiger-mode: TRIGGER device!!
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
F.Zappa, S.Cova, M.Ghioni, US patent
iAQC: integrated Active Quenching CircuitiAQC: integrated Active Quenching Circuit
iAQC, oltre la miniaturizzazione…
• Capacità ridotte
• Carica di valanga ridotta
• Afterpulsing ridotto
Active Quenching Circuit
• Tempo morto corto e ben definito
• High counting rate > 1 Mc/s
• Impulso Standard d’uscita
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Modulo SPTM2: Diagramma a BlocchiModulo SPTM2: Diagramma a Blocchi
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Planar thin Si-SPADPerkinElmer SPCM (SLIKTM diode)
Risoluzione TemporaleRisoluzione Temporale
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Risoluzione Temporale @500nmRisoluzione Temporale @500nm
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Modulo SPTM2Modulo SPTM2
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Risultati SperimentaliRisultati Sperimentali
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
Misure su Singola MolecolaMisure su Singola Molecola
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Ivan Rech
Photon-timing Detector Module For Single-molecule SpectroscopyPhoton-timing Detector Module For Single-molecule Spectroscopy
“Single-Molecule Protein Dynamics on Multiple Time Scales Probed by Electron Transfer” Haw Yang*, Guobin Luo*, Pallop Karnchanaphanurach*, Tai-Man Louie‡, Ivan Rech°, Sergio Cova°, Luying Xun‡ and X. Sunney Xie** Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138. ‡ School of Molecular Biosciences, Washington State University, Pullman, WA 99164. ° Dipartimento di Elettronica e Informazione, Politecnico di Milano
Science, Vol.302, 262-266, 10 Oct. 2003.
“Photon-Timing Detector Module for Single-Molecule Spectroscopy with 60ps Resolution” Ivan Rech, Guobin Luo*, Masimo Ghioni, Sergio Cova, Haw Yang*, X. Sunney Xie*Dipartimento di Elettronica e Informazione, Politecnico di Milano *Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA
IEEE Journal of Selected Topics in Quantum Electronics, in press, 2004