pharmaceutico-analytical study of taleshwara...
TRANSCRIPT
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018
A A M J Anveshana Ayurveda Medical Journal
www.aamj.in ISSN: 2395-4159
Research Article
PHARMACEUTICO-ANALYTICAL STUDY OF TALESHWARA RASA
Lavanya S. A*
A b s t r a c t
Taleshwara Rasa is an eccentric formulation which is favourable in the management of 18
types of Kushta. Regardless of its betterment in the management of Kushta no research work
has been carried out till date. The main aim of this study was preparation of Taleshwara Rasa
as disclosed in the classics & Physico-chemical analysis of Taleshwara Rasa. Taleshwara
Rasa was processed using Kajjali, Haratala Bhasma, Shuddha Tankana, Shuddha
Vatsanabha , Shuddha Dhattura beeja, Jaatiphala, Bakuchi Beeja Churna, Nimba beeja
majja churna, Shunti Churna, Maricha churna, Pippali churna. The above ingredients were
mixed to get a homogenous mixture of Taleshwara Rasa which was given 7 Bhavana with
Guduchi patra swarasa followed by 7 bhavana of Dhattura patra swarasa. After bhavana a
bolus was prepared out of the mixture and Dhattura patra veshtana was done followed by
gomaya lepa and subjected to Kukkuta puta. The Physico chemical analysis of Taleshawara
Rasa before (TR-BB) and after bhavana (TR-AB) and after puta (TR-AP) was done.
Key words – Taleshwara Rasa, XRD, FTIR, SEM-EDAX.
*PG Scholar, Dept of Rasashastra and Bhaishajya Kalpana, Taranath Govt. Ayurvedic Medical College,
Bellary, Karnataka, India.
CORRESPONDING AUTHOR
Dr. Lavanya S. A. Dept of Rasashastra and Bhaishajya Kalpana,
Taranath Govt. Ayurvedic Medical College,
Bellary, Karnataka, India.
Email: [email protected]
http://aamj.in/wp-content/uploads/Volume4/Issue4/AAMJ_1798_1807.pdf
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1799
INTRODUCTION
asashastra is a branch of Ayurveda which deals
with metallo-mineral preparations aimed at
achieving Lohavada & Dehavada. These
preparations became acceptable due to its assimilatory
property in the minute doses. There are mainly 4 Rasa
Kalpas namely Khalviya, Parpati, Pottali & Kupipakwa
Rasayana. Taleshwara Rasa1(TR) is one such Khalviya
rasayana mentioned in the classics which have a distinct
method of preparation than any other formulation that is
beneficial in the management of Ashtadasha Kushta2. There
are numerous formulation in the name of Taleshwara Rasa
with different suffix and prefix. In the present study the
formulation is taken from the text Rasa Manjari.
The analytical study reveals the chemical composition of the
formulations as well as their concentration. By this it helps
to ensure safety limits and accuracy of the drug. Physico-
chemical analysis of the drugs is carried out by using current
analytical methodologies for understanding and
interpretation of physico-chemical changes occurring during
and after pharmaceutical processing. The Physico chemical
analysis of Taleshawara Rasa before bhavana (TR-BB) and
after bhavana (TR-AB) and after puta (TR-AP) was done.
MATERIALS AND METHODS
Pharmaceutical stride involved in preparation of
Taleshwara Rasa:
1. Preparation of homogenous mixture of Taleshwara
Rasa ingredients.
2. 7 Bhavana was given with Guduchi patra swarasa
followed by 7 bhavana of Dhattura patra swarasa
3. Preparation of bolus out of bhavita mixture of
Taleshwara Rasa.
4. Dhattura patra veshtana to the bolus followed by
Gomaya lepana.
5. Subjecting the Gomaya Lepita bolus to Kukkuta
puta.
Table 1: Showing the ingredients and their Quantity of
Taleshwara Rasa
Ingredients Quantity (g)
Shuddha Parada 14
Shuddha Gandhaka 21
Haratala Bhasma 70
Shuddha Tankana 70
Shuddha Vatsanabha 10.5
Shuddha Dhattura Beeja Churna 7
Nimba Beeja majja 7
Bakuchi beeja 21
Shunti 21
Maricha 28
Pippali 21
Jaatiphala 70
Procedure:
Kajjali and Haratala bhasma was taken in a clean khalwa
yantra and triturated for 2 hours to prepare a homogenous
mixture. To that Shuddha Tankana was added and mardana
was done for 2 hours. To this mixture the churna of
Jaatiphala followed by the churna of vatsanabha, dhattura
beeja, nimba beeja majja, bakuchi beeja, shunti, maricha
and pippali were added and triturated well to form a
homogenous mixture.
Bhavana of TR mixture with Guduchi patra swarasa:
Extraction of Guduchi patra swarasa:
Fresh and matured leaves of guduchi were collected and
washed to remove the impurities. Later the leaves were
taken in a mixer jar and grinded well into a paste. Then the
paste was placed on a two-folded Cora cloth and squeezed
to obtain fresh swarasa.
Procedure:
To the mixture of TR required quantity of guduchi patra
swarasa was added such that the mixture is completely
immersed in the liquid and mardana was carried out for 6
hours. Same procedure was repeated for 6 more times.
Bhavana of TR mixture with Dhattura patra swarasa:
Extraction of Dhattura patra swarasa:
Fresh and matured leaves of dhattura were
collected and washed to remove the impurities. Later the
leaves were taken in a mixer jar and grinded well into a
paste. Then the paste was placed on a two-folded Cora cloth
and squeezed to obtain fresh swarasa.
Procedure:
To the mixture of TR required quantity of dhattura patra
swarasa was added such that the mixture is completely
immersed in the liquid and mardana was carried out for 6
hours. Same procedure was repeated for 6 more times.
Observations:
The colour of the mixture which was dark blackish grey
colour after adding haratala bhasma to kajjali turned to
light grey colour after adding Shuddha Tankana.
After adding the churna of Kashtoushadhis the colour
was Light grey.
Characteristic odour of Jaatiphala was felt during the
mardana of TR mixture
During the extraction of Guduchi swarasa, sliminess
was felt and the swarasa was green in colour with
watery consistency.
During the extraction of Dhattura patra swarasa
characteristic odour of dhattura was felt and the colour
of swarasa was dark green in colour. Consistency was
thicker when compared to the guduchi patra swarasa.
Total duration taken for the completion of homogenous
mixture of TR was 4 hours.
R
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1800
In total 14 bhavana was given to the TR mixture. Each
Bhavana was carried out for 6 hours.
The amount of Swarasa used was reduced day by day.
Total quantity of Guduchi patra swarasa used was
2.410 ltrs and dhattura patra swarasa used was 2.485
ltrs.
The trituration strokes were 30-strokes/minute in initial
stage of Bhavana and it reduced to 20 strokes / minute
when the fluid portion decreased.
Precautions
Khalva Yantra should be clean and dry before the
process starts.
Daily fresh swarasa was used.
The pestle should be rotated entire length of Khalva
Yantra in both clock and Anti clock- wise directions.
Table 2: Showing the consumption of swarasa for 14 bhavana
Amount of Guduchi patra Swarasa used (in ml) for 7
bhavana
Amount of Dhattura patra Swarasa used (in ml) ) for 7
bhavana
400 ml 410 ml
375 ml 390 ml
360 ml 375 ml
345 ml 360 ml
330 ml 345 ml
310 ml 315 ml
290 ml 290 ml
Putana Karma for TR Mixture:
1. Preparation of Bolus of TR Mixture:
After giving Bhavana when the mass attained semisolid
consistency it was taken out from the khalwa yantra and
made into a bolus and kept for drying. After 3 hours of
drying small cracks started appearing on the surface of the
bolus. Then the bolus was subjected for further steps.
2. Dhattura Patra Veshtana for the Bolus:
Fresh and matured leaves of Dhattura was collected and
washed with water. It was then kept for drying. Later 5
leaves were selected and placed in a circular manner on the
stainless steel plate and bolus was placed over the leaves.
Then the bolus was covered with the leaves and secured
using a Cotton thread.
3. Gomaya Lepana for Bolus:
Freshly collected gomaya was placed on a plate and a base
of 2.5cm thickness was created. Above this the dhattura
patra veshtita TR was placed and the lepana of gomaya was
done with a thickness of 2.5 -3 cm. It was placed
undisturbed for drying. The surface of the Gomaya lepita TR
was changed every now and then in order to provide
complete drying of the lepana. It took 5 days for complete
drying of gomaya lepa.
4. Kukkuta Puta:
a) Size and Shape :Kukkuta Puta was
given by using the pit of following dimensions.
Size Of Pit : 31 cm Height , 31cm depth and
31cm width
b) Fuel : 30 cow dung cakes,
totally dried, each cowdung with Following dimension
Diameter : 13cm (on average)
Thickness : Centrally 4cm, periphery 2cm
Weight : 100grams (approx)
Total Weight : 3 kg
18 cow dung cakes were placed below the bolus and 12
were placed above to ensure uniform degree of heat.
c) Mode of Heating
Fire was ignited by keeping four small camphor balls
on all four sides.
Thermocouple tip was placed on the side of the Bolus
(upper 1/3rd of Puta).
Temperature obtained during Puta was recorded with
the help of Pyrometer.
After Swanga sheeta, the bolus was taken out of pit and
layers of gomaya and dhattura patra were scraped
carefully with knife.
The putita TR was collected, weighed, powdered and
stored in an airtight container.
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1801
Graph No 1: Showing Time Vs Temperature during Kukkuta puta of TR
Table 3:- Showing in process testing of Putita TR.
Sl.No. Name of Test Result
1 Colour Black
2 Taste Tasteless
3 Touch Mrudu
4 Appearance Fine powder
5 Odour Burnt Smell
Observations:
Slight cracks started appearing on the
surface of the bolus after 3 hours of
drying.
Colour of the bolus was Greenish Dark
Grey.
It was difficult to do lepana of gomaya
over the dhattura patra veshtana as the
gomaya was freshly collected.
It took 5 days for the complete drying of
gomaya lepa.
Kukkuta Puta:
The rise in temperature was up to 458o C for 25-30
minutes.
After Puta the bolus was in same form with
change in Colour.
Time taken for complete burning was 5-6 hours.
The bolus was completely burnt and the outer layer
of gomaya and dhattura patra was brittle in nature.
The inner bolus of TR which was soft and in
semisolid consistency had become brittle and the
colour was changed to black.
When the bolus was broken into two halves the
inner surface was shiny and brittle.
Table 4: Showing Pharmaceutical Results of TR
Analytical Study of Taleshwara Rasa:
Table 5: Showing Organoleptic & Physico-chemical analysis of TR
24
395458
386320
235142
65 28
0100200300400500
10:00 11:00 11:30 12:00 01:00 02:00 03:00 04:00 05:00Tem
pe
ratu
re in
0c
Time
Time and Temperature during kukkuta Puta of
TR
Result In Grams
Initial weight of TR mixture 360.5
Weight of TR mixture after Bhavana 410
Total Gain after bhavana 49.5
Weight after Puta 343.5
Loss of drug after puta 66.5
Parameters TR-BB TR-AB TR-AP
Colour Ash coloured Greenish Brown Brown
Touch Fine powder,
Amorphous
Fine powder,
Amorphous
Fine powder,
Amorphous
Odour Characteristic Characteristic Characteristic
Taste Pungent Pungent Tasteless
Total Ash w/w 43.50% 35.70% 48.50%
Acid Insoluble Ash w/w 41.25% 5.47% 6.85%
Water soluble Ash w/w 18.50% 29.50% 27.50%
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1802
XRD3 Results of TR-BB, TR-AB & TR-AP:
XRD peaks of TR-BB, TR-AB & TR-AP samples which were compared with standard D-space JCPDF values confirmed
the presence of Metacinnabar (HgS) in Cubic, Sulfur (S) in Trigonal, Mercury Oxide (HgO) in Hexagonal, Arsenic
Sulfide (As2S3) in Unknown, Calcite (CaCO3) in Trigonal, Calcium Arsenate ( Ca2(AsO4)2) in monoclinic and
Tincalconite (Na2B4O710H2O) in Trigonal crystal system.
Graph No 2: Peaks of TR-BB XRD Graph No 3: Peaks of TR-AB XRD
Graph No 4: Peaks of TR-AP XRD
Table 6: Showing Comparative SEM-EDX4 results of TR-BB, TR-AB & TR-AP
TR-BB TR-AB TR-AP
Element Mass% At % Mass% At % Mass% At %
Hg 0.66 0.05 2.65 0.19 3.86 0.40
S 0.82 0.42 1.74 0.80 3.87 2.48
Collected Data-3
0
500
1000
1500
2000
20 40 60 80
Theta/2-Theta[deg]
Inte
nsity
[cps]
Collected Data-2
0
500
1000
1500
2000
20 40 60 80
Theta/2-Theta[deg]
Inte
nsity
[cps]
Collected Data-1
0
500
1000
1500
2000
20 40 60 80
Theta/2-Theta[deg]
Inte
nsity
[cps]
Loss on Drying at 1050c 1.26% 3.55% 0.90%
pH 9.64 9.14 9.19
Alcohol Soluble extractive 21.38% 16.56% 8.95%
Water Soluble Extractive 26.54% 34.54% 32.75%
Mercury 3.50% 2.95% 6.10%
Free Mercury Nil Nil Nil
Mercurous Mercury 00.25% 00.38% 00.19%
Mercuric Mercury 3.25% 2.57% 5.91%
Sulphur 8.58% 6.41% 6.95%
Free Sulphur 00 00 00
Sulphide 8.51% 6.10% 6.50%
Sulphate 0.07% 0.31% 0.45%
Boron 2.67% 2.02% 2.91%
Arsenic 16.55% 14.75% 18.90%
Arsenite 14.65% 12.60% 14.45%
Arsenate 18.90% 14.45% 4.45%
Sodium 3.10% 3.16% 3.50%
Calcium 0.90% 1.43% 1.50%
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1803
B 26.58 40.06 22.64 30.95 13.70 26.04
As 20.31 4.42 11.15 2.20 26.92 7.38
Na 3.58 2.54 2.04 1.31 0.40 0.36
Ca 4.30 1.75 3.90 1.44 15.11 7.75
C 21.01 28.50 40.89 50.32 28.66 49.02
O 21.45 21.85 13.20 12.20 3.87 2.48
Fe 0.17 0.05 0.18 0.05 0.29 0.11
Zn 0.26 0.06 0.25 0.06 0.61 0.19
K 0.49 0.20 1.09 0.41 2.17 1.14
Cu 0.38 0.10 0.28 0.06 0.54 0.17
Graph No 5: Depicting EDAX of TR-BB Graph No 6: Depicting EDAX of TR-AB
Graph No 7: Depicting EDAX of TR-AP
FTIR5:
FTIR analysis of TR-BB & TR-AP shows it contains organic functional group like Alcohol, Amine, Amide, Acid, Alkene,
Alkane, Aromatic, Acid, Carbonyl, Nitro, Alkyl Halide, Aromatic, Ester and Ether.
FTIR analysis of TR-AB shows it contains organic functional group like Alcohol, Amine, Amide, Acid, Alkene, Alkane,
Aromatic, Acid, Carbonyl, Nitro, Alkyl Halide, Aromatic, Alkyne, Thiocyanate, Ester and Ether.
Graph No 8: TR-BB Graph No 9: TR-AB
5007501000125015001750200025003000350040001/cm
30
40
50
60
70
80
90
100
%T
3350.3
5
3016.6
72922.1
62852.7
2
2625.1
2
2509.3
9
2345.4
4
1735.9
3
1656.8
51637.5
6
1421.5
41371.3
91344.3
8
1130.2
91103.2
81080.1
41004.9
1945.1
2
873.7
5831.3
2771.5
3
713.6
6 464.8
4
TR-BB
5007501000125015001750200025003000350040001/cm
30
40
50
60
70
80
90
100
%T
3550.9
53471.8
7
3300.2
0
2920.2
32852.7
2
2522.8
9 2281.7
9
2164.1
3
1734.0
11668.4
31639.4
9
1537.2
7
1444.6
81413.8
2
1340.5
3
1109.0
7
1035.7
7
871.8
2839.0
3
717.5
2
TR-AB
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1804
Graph No 10: TR-AP
Particle Size Results6:
Table 7: Showing Particle Size Results of TR-BB, TR-AB, TR-AP
Effective Diameter(nm)
Sample Mean diameter(nm) Standard error Effective diameter(nm)
TR-BB 839.6 42.6 821.8
TR-AB 1055.7 97.2 1183.0
TR-AP 757.1 19.4 764.8
HPTLC7 results
Table 8:- Showing Rf values of TR-BB, TR-AB, TR-AP at 254nm & 366nm
At 254 nm At 366 nm
TR-BB TR-AB TR-AP TR-BB TR-AB TR-AP
0.18(D. green) 0.18(D. green) 0.18(L. green) 0.04 (F. blue) 0.04 (F. blue) 0.04 (F. blue)
0.33(D. green) 0.33(D. green) 0.33(L. green) 0.08 (F. blue) 0.08 (F. blue) 0.08 (F. blue)
0.40(D. green) 0.40(D. green) 0.40(L. green) 0.16 (F. blue) 0.16 (F. blue) 0.16 (F. blue)
0.53 (L. green) 0.53(L. green) 0.53(L. green) 0.27 (F. blue) 0.27 (F. blue) 0.27 (F. blue)
0.60 (L. green) 0.60(L. green) 0.60(L. green) 0.37 (F. blue) 0.37 (F. blue) 0.37 (F. blue)
0.67(D. green) 0.67(D. green) 0.67(L. green) 0.44 (F. blue) 0.44 (F. blue) 0.44 (F. blue)
0.78 (Green) 0.78 - 0.55 (F. blue) 0.55 (F. blue) 0.55 (F. blue)
0.86 (L. green) 0.86 - 0.61 (F. blue) 0.61 (F. blue) 0.61 (F. blue)
Table 9:- Showing Rf values of TR-BB, TR-AB, TR-AP after post derivatisation
TR-BB TR-AB TR-AP
0.10 (Purple) 0.10 (Purple) 0.10 (Purple)
0.20 (Purple) 0.20 (Purple) 0.20 (Purple)
0.29 (Purple) 0.29 (Purple) 0.29 (Purple)
0.33 (Purple) 0.33 (Purple) 0.33 (Purple)
0.38 (Purple) 0.38 (Purple) 0.38 (Purple)
0.43 (Purple) 0.43 (Purple) 0.43 (Purple)
0.53 (Purple) 0.53 (Purple) 0.53 (Purple)
0.56 (Purple) 0.56 (Purple) 0.56 (Purple)
0.69 (Purple) 0.69 (Purple) 0.69 (Purple)
0.80 (Purple) 0.80 (Purple) -
0.89 (Purple) 0.89 (Purple) -
5007501000125015001750200025003000350040001/cm
30
40
50
60
70
80
90
100
%T
3332.9
9
2922.1
62854.6
5
2524.8
2
1861.3
1
1653.0
0
1436.9
7
1105.2
1
873.7
5837.1
1
711.7
3
626.8
7
TR-AP
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1805
Graph No 11: 3D Chromatogram at 254nm Graph No 12: 3D Chromatogram at 366 nm
Graph No 13: 3D Chromatogram at 620nm
DISCUSSION
Pharmaceutical study: Preparation of TR mixture:
TR was prepared step by step. Kajjali and Haratala
bhasma was taken in a clean khalwa yantra and triturated for
2 hours to prepare a homogenous mixture. To that of
Shuddha Tankana was added and mardana was done for 2
hours. To this mixture the churna of Jaatiphala followed by
the churna of Vatsanabha, dhattura beeja, nimba beeja
majja, bakuchi beeja, shunti, maricha and pippali were
added and triturated well to form a homogenous mixture.
Bhavana of TR mixture with Dhattura Patra & Guduchi
Patra swarasa
To the mixture of TR required quantity of dhattura
patra swarasa was added such that the mixture is
completely immersed in the liquid and mardana was carried
out for 6 hours. Same procedure was repeated for 6 more
times. It is then followed by 7 bhavana of Guduchi patra
swarasa.
In a study it has been explained that a total of 12
elements have been reported quantitatively that includes
Cu, Co, Ni, Mn, Zn, Fe, Na, K, Ca, Mg, P & Al7.
Dhattura leaves are mineral rich than their seeds and
flower counterparts. This is suggestive that dhattura
patra swarasa not only helps in relieving the disease
condition but also imbibe few of these trace elements
required to the body into the formulation.
Phytochemical screening of leaf of guduchi indicates
the presence of alkaloids, glycosides, carbohydrates,
saponins, phenols, phytosterols, tannins and amino-
acids and suggested that it is an important source of
bioactive compounds that may supply novel medicine.
It is also richer in anti-oxidants which are helpful in
counteracting the free radicals of the disease.
Both Dhattura and Guduchi have Tikta rasa, Laghu,
ruksha guna, Ushna veerya which helps in removal of
kapha dosha and does kledaharana that are considered
as the root cause of the disease Kushta.
Putana karma of TR:
A bolus of bhavita TR was prepared and dhattura
patra veshtana was done followed by gomaya lepa and
allowed for drying. Then it was subjected for kukkuta puta
once.
The layer of Dhattura patra and gomaya act as
protective covering in order to avoid the direct contact
of heat with the drug mixture. If direct heat is given
then the formulation might get carbonised as it contains
major amount of organic compounds as ingredients.
Gomaya has Kashaya rasa and ushna veerya which
helps in kleda shoshana of the body. As it is subjected
to puta there is a chance of chemical reaction occurring
between the Dhattura patra, gomaya and drug mixture
due to which the medicinal properties of dhattura and
gomaya might be induced into the drug.
After 14 bhavana due to the usage of organic media for
levigation the particle size of the drug might increase which
in turn decrease the absorption in the body. In putana karma
organic compounds undergo carbonisation and the particle
size gets reduced which helps in easy absorption and
assimilation of the drug. Hence puta is given after the
completion of Bhavana.
Analytical Study:
Total ash value helps to know about inorganic material
in the test drug. Total ash value of TR-BB, TR-AB &
TR-AP is found to be 43.50%, 35.70% & 48.50%. This
indicates less amount of inorganic material in the
sample, and 56.5%, 64.3% & 51.5% is organic that is
bio-human available products. The ash value after
Bhavana is decreased to 35.70% which indicates that
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1806
the amount of organic material has become more after
Bhavana which is suggestive of the presence of organic
compound of the Bhavana drugs also.The ash value
after putana karma has been increased to 48.50% which
indicates that the organic matter present has been
carbonised due to the heat supplied during putana
karma.
Acid insoluble ash of TR-BB, TR-AB, TR-AP are 41.25%,
5.47% and 6.85% respectively indicating that 93.15% of
the drug is easily soluble in the gastric environment.
It indicates selective media of drug administration. Water
soluble ash value of TR-BB, TR-AB, TR-AP is 18.50%,
29.50% & 27.50% respectively indicating less solubility
of the drug in water media. TR is a herbo-mineral
formulation; the presence of inorganic substance in the
formulation might make this drug less soluble in the
water media.
The least loss on drying at 1050C, the better will be the
drug. In the present study TR-BB, TR-AB, TR-AP
possesses 1.26%, 3.55% and 0.90% respectively. Hence
it can be stated that all have very less amount of moisture
content and very rare chance of bacterial and fungal
growth. The drug is having least hydroscopic activity
with less chance of contamination of drug. Concurrently
it can be stated that the shelf life of the drug in the
present study is more.
Water soluble and Alcohol Soluble extractives indicates
the mode of administration of the drug. Alcohol soluble
extractives of TR-BB, TR-AB & TR-AP are 21.38%,
16.56% & 8.95% whereas water soluble extractives are
26.54%, 34.54% & 32.75%. This indicates that water is
not suitable media for the TR administration and
extraction.
The percentage variation of arsenic compound may be
due to formation of various compounds with oxygen and
sulphur.
The percentage of carbon has been increased in TR-AB
compared to TR-BB; it may be due to the addition of
organic material. The percentage of carbon decreased in
TR-AP compared to TR-AB, it may be due to the
carbonization of organic substances during putana
karma.
The reduction in oxygen percentage gradually in TR-BB,
TR-AB, and TR-AP may be due to the oxidation process.
The elements like Boron, Sodium are detected due to
Tankana which is one of the major ingredients of TR.
The increase in the percentage of sulphur and calcium
may be due to conversion of elemental calcium and
sulphur into compound form.
The other minor elements like K, Fe, Zn, Cu may be due
to the 7 Bhavana of TR with Dhattura patra swarasa as
mentioned earlier dhattura leaf are mineral rich
containing 12 elements.
The increase in particle size after Bhavana may be due to
the Bhavana of the drug with the swarasa of organic
material as the particles of organic material will not
reduce to nanometre scale. The reduction in particle size
of TR-BB compared to TR-AP may be due to putana
karma.
In present study HPTLC of the drug mixture is compared
with the final product. The final product shows the
presence of various herbal ingredients present in the
formulation and hence show that the preparation is not
adulterated.
CONCLUSION
Taleshwara Rasa is one of the Khalviya rasayana. There are
many references with different prefix available with
variation in the ingredients method of preparation and
dosage. Among them, Rasa Manjari reference was selected
for the preparation. In total there was 18.44% loss in the
weight during the pharmaceutical preparation of the drug.
Chemically Taleshwara Rasa is considered as a compound
of Metacinnabar, Sulfur, Mercury Oxide, Arsenic sulphide,
Calcite, Calcium Arsenate, Tincalconite with the organic
compounds with their functional groups like Alcohol,
Amine, Amide, Acid, Alkene, Alkane, Aromatic, Acid,
Carbonyl, Nitro, Alkyl Halide, Aromatic, Ester and Ether.
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REFERENCES
1. Acharya Shalinath. Rasa Manjari. Hindi commentary by Prof.
Siddhi Nandan Mishra, 2nd Edition. Varanasi: Published by
Chaukambha Orientalia; 2003. 6th Chapter, Verses 252 – 256,
113 pp.
2. Acharya Agnivesha. Charaka Samhita. Edited byVaidya
Yadavji Trikamji Acharya. Varanasi: Chaukambha Sanskrit
Samsthana; 2008. Chikitsasthana, 7th Chapter, Verse 13, 451
pp.
3. www.unm.edu/xrd/xrdbasics.pdf.
4. serc.carleton.edu.en.wikipedia.org/wiki/Energy-dispersive_X-
ray_spectroscopyCachedSimilar
5. www.wcaslab.com,mmrc. caltech. edu/FTIR/FTIRintro.pdf.
6. www.Zeta potential analysier.pdf.
7. Kasture A.V. Mehadik R. et.al. Pharmacopieal Analysis.
Pune: Nirali Prakashan; 2002.vol 5,13-18pp.
Source of Support: Nil.
Conflict of Interest: None declared
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How to cite this article: Lavanya S. A: Pharmaceutico-
Analytical Study Of Taleshwara Rasa. AAMJ 2018; 4:
1798 – 1807.
Lavanya S. A: Pharmaceutico-Analytical Study Of Taleshwara Rasa
AAMJ / Vol. 4 / Issue 4 / July – Aug 2018 1807
PHOTOS
Fig No:1 Fig No:2 Fig No:3
Parada & Gandhaka for Kajjali Kajjali Haratala Bhasma
Fig No:4 Fig No:5 Fig No:6
Shuddha Tankana Shuddha Vatsanabha Shuddha Dhattura
Fig No: 7 Fig No:8 Fig No:9
Ingredients of TR Bhavana of TR Mixture Bolus prepared out of
TR Mixture
Fig No:10 Fig No:11 Fig No:12
Dhattura patra veshtita Gomaya Lepita TR bolus Gomaya lepita TR bolus placed
TR Bolus in kukkuta puta pit
Fig No: 13 Fig No: 14 Fig No: 15
Gomaya Lepita TR Bolus TR bolus after puta Taleshwara Rasa