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8/18/2019 Pharma Herbal b2

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A Study on the Effects ofMentha arvensis on the

Gastrointestinal Motility ofMice

Pharma Herbal B-2


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Introduction

Peppermint or Mint • common name for the plant scientifically classified under t

Lamiaceae family, specifically of the Nepetoideae subfamithe genus Mentha.

•2 Species commonly used:• Mentha arvensis

• Mentha piperita


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Known medical uses:

•exuding virucidal effects on Herpes Simplex Virus type 1 and

•antispasmodic in elderly patients undergoingesophagogastroduodenoscopy

• treatments for migraine, upper respiratory tract diseases acardiovascular diseases


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Gastrointestinal uses:

•studies have shown its medical prospect in the treatment ofgastrointestinal diseases such as irritable bowel syndrome (

• Studies which test Mentha’s effect on gastric emptying alsomedical prospect.

•Despite the existence of these studies, there is still no clear

as to the effects of Peppermint on the gastrointestinal syste


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Statement of the problem:

The purpose of the study is to elicit the gastrointestinaeffects of the methanolic extract of Mentha arvensis inintestines of mice.


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Research question:

Does the methanolic extract of Mentha arvensis exert signdecrease of gastrointestinal motility in mice when comparmice given negative control (NSS) and positive control(Loperamide)?


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Significance:

•

Expand knowledge about the exact effects of Mentha arvengastrointestinal system.

•provide further evidence on medicinal uses of peppermint ftreatment of gastrointestinal diseases.

•The information gathered will be significantly important to t

wish to do further studies on Mentha arvensis.


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Scope and Limitations

• study is limited to the use of M. arvensis leaf extracts as agdecrease gastric motility in the intestines of the mice

• study limits itself to the gastrointestinal motility of the micdoes not take into account its effects on other smooth mu


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Methodology

• Experimental design

Experimental animal: 18 albino mice

Reagents and chemicals:

• Methanol

•Loperamide

• Normal Saline Solution

• India Ink

Plant material: dried Mentha arvensis


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Preparation of the extract

Methodology


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Experiment setup

Methodology


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Results


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Results


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Results


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Results


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Results


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Discussion

All of the statistical results have shown that there was no sigdifference between the comparison of the positive control anegative control.

The experimental p (0.3032) was greater than set alpha=0.0


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Number of factors could be taken into consideration for thisuch as the positive control (Loperamide) having a largevariance as evidenced by the highly varying values per mous

Only the negative control (NSS) was able to elicit smal

among the three set-ups.


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Sudden change of route of administration

Dosage and content in a solution as exemplified in a studyYadav & Kalia (2010) revealed that utilizing 2% as comto 3%and 4% , in a solution with banana leaves and

will yield an optimal effect that not only relaintestines, but also yield an increase in energy


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Yadav, Yadav & Kalia (2010)Further, the study emphasized the importance of storage duand temperature as factors that can alter the effectiveness arvensis


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M. piperita, M. aquatica, M. longifolia, serves to gastrointestinal tract due to its calcium channel block(Mikhaili et al., 2013; Beesley et al., 1996); however, M. arvspecies used in this study did not yield an evidence that wthe same relaxing effect.


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Conclusion

•

No sufficient evidence to reject null hypothesis

• Oral gavage administration of M. arvensis, similar to Loper

and NSS , exert no significant effect on the motility of the

gastrointestinal tract of the mice used in this experiment.


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Recommendations

• replicate the study but with an alternative procedure suchintraperitoneal administration

• utilizing larger sample size• variations in the percentage concentration (dosage) of the

arvensis extract

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