pGLO followup and term 4!!!

Announcements: Before April Break you must schedule a

5 minute conference with me after school or at 7:45. List is on the board. This is a HW assignment to set goals for term 4!

pGLO lab is due Friday 4/11 Extra help is available next Tuesday from

my senior helpers! You must have a draft to work with!

Questions to consider for introduction: What do the terms “bioengineering” and

“cloning” mean? Where is the gene originally from? What does the plasmid contain? How will we get the plasmid into the

bacteria? What will activate the production of GFP

in bacteria?

Peer Review of Introduction Advanced: Discusses concepts and links to

the specific goal in this lab!!! Proficient: Discusses bioengineering,

cloning, plasmids and heat shock. Links them to eachother but does not tie to the purpose of bioengineering in this lab.

Needs Improvement: Describes most of the concepts but does not link them to eachother…or…misses concepts

Give your partner an assessment and write a sentence or two to explain why!

Collection of Data For the purposes of this lab, we are

going to call transformation efficiency the number of colonies produced!

You need a table to show the results on all four of your plates as well as a graphical representation (picture, etc).

For class data, we will look at our “experiment within an experiment” Temperature of “cloning procedure” Transformation efficiency How could you choose to display

these results?

Conclusions: What did we learn about the plasmid

system we used based on our results? What were some reasons for variation

within the data…is there anything we could modify?

What else could apply our new found knowledge of bioengineering to? Use the guiding questions on your lab

sheet but don’t let them limit you!!!