pgem-t easy

Sap I 394 Nsi I 123 BstX I 111 Sac I 105 Nde I 96 Sal I 90 HinC II 90 Pst I 84 NotI 71 EcoRI 66 EcoR V 58 EcoRI 48 Sac II 46 Nco I 37 Sty I 37 NotI 33 Sph I 22 Aat II 16 AlwN I 928 Ahd I 1405 Bsa I 1477 Gsu I 1495 Xmn I 2005 Rsa I 1889 Sca I 1888 Bpm I 1495 Gsu I 1495 Bsa I 1477 Dra III 2599 BsaA I 2602 Nae I 2705 lacZa AmpR f1ori pGEM-T Easy 3015 bp Plasmid name: pGEM-T Easy Plasmid size: 3015 bp Constructed by: Promega Corporation, Madison, WI. Construction date: Comments: T7 RNA Polymerase transcription initiation site 1 SP6 RNA Polymerase transcription initiation site 141 T7 RNA Polymerase promoter (-17 to +3) 2999-3 SP6 RNA Polymerase promoter (-17 to +3) 139-158 multiple cloning region 10-128 lacZ start codon 180 lac operon sequences 2836-2996, 166-395 lac operator 200-216 beta-lactamase coding region 1337-2197 phage f1 region 2380-2835 binding site of pUC/M13 Forward Sequencing Primer 2956-2972 binding site of pUC/M13 Reverse Sequencing Primer 176-192 The pGEM(R)-T Easy Vector has been linearized with EcoRV at Base 60 of this sequence (indicated by an asterisk *) and a T added to both 3' -ends.

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Sap I 394

Nsi I 123

BstX I 111Sac I 105

Nde I 96Sal I 90

HinC II 90Pst I 84

NotI 71EcoRI 66

EcoR V 58EcoRI 48

Sac II 46Nco I 37

Sty I 37

NotI 33

Sph I 22Aat II 16

AlwN I 928

Ahd I 1405Bsa I 1477

Gsu I 1495

Xmn I 2005

Rsa I 1889Sca I 1888

Bpm I 1495Gsu I 1495

Bsa I 1477

Dra III 2599BsaA I 2602

Nae I 2705

lacZa

AmpR

f1ori

pGEM-T Easy3015 bp

Plasmid name: pGEM-T EasyPlasmid size: 3015 bpConstructed by: Promega Corporation, Madison, WI. Construction date: Comments: T7 RNA Polymerase transcription initiation site 1 SP6 RNA Polymerase transcription initiation site 141 T7 RNA Polymerase promoter (-17 to +3) 2999-3 SP6 RNA Polymerase promoter (-17 to +3) 139-158 multiple cloning region 10-128 lacZ start codon 180 lac operon sequences 2836-2996, 166-395 lac operator 200-216beta-lactamase coding region 1337-2197phage f1 region 2380-2835binding site of pUC/M13 Forward Sequencing Primer 2956-2972binding site of pUC/M13 Reverse Sequencing Primer 176-192

The pGEM(R)-T Easy Vector has been linearized with EcoRV at Base 60 of this sequence (indicated by an asterisk *) and a T added to both 3' -ends.

Molecular Cloning, Tissue Distribution, and Functional ... · Zero Blunt TOPO PCR CloningKit (Invitrogen) or pGEM-T easy vectors using a pGEM-T Easy Vector System (Promega, Madison,

Resurrection of Anopheles goeldii from synonymy with Anopheles ... · directly sequenced; 18 were cloned into pGem-T Easy Vector (Promega, Madison, WI, USA). Two to four posi - tive

New Changgp,pes in pests, plants and their interactions as drivers of … · 2015. 8. 4. · T529/02 P2 1000 TYLCV-[IT:Sic:05] TYLCV-IL pGEM-T BglII digestion pGEM T 1 K b T Y L C

pgem-t easy vector systems protocol

Aae, an Autotransporter Involved in Adhesion of ...of Vermont, using pVT1566 as template and, for sequencing primers, ﬁrst the SP6 and T7 primers from pGEM-T Easy and then the primers

misterios da lemuria e da atlantida Laercio fonseca (senha pgem)

Estudo de elementos transponíveis em Puccinia psidii ... · 3.2.2.3.2 Clonagem em vetor pGEM®T Easy Vector ... Estudo de elementos transponíveis em Puccinia psidii Winter, agente

No flip-open cover Kein Aufklapper T! Easy C 214 ... · 24.06.2003 T-Eas214_U_1.fm Gerätebezeichnung: T-Easy C 214 T! Easy C 214 Bedienungsanleitung Rücken ca. 3 mm Kein Aufklapper

Pgem q4 2014 earnings slides final

Atlantida e Lemuria (Senha=PGEM)

r o t e o m ics&B o f P Journal of Asthana et al., J Proteomics … · 2019. 7. 9. · to clone hexon gene from all the three isolates into pGEM-T Easy vector, sequenced and analyzed