PEDRIGREE AND GENETIC ANALISYS OF THE ISTRIAN

CATTLE

Ivanković A.1, Ramljak J.1, Šubara G.2, Kelava N.1, Prekalj G.2

(1)   Department of Animal Production and Technology, Agricultural Faculty, University of Zagreb, Svetošimunska 25, 10 000 Zagreb, Croatia.

(2)   AZRRI – Agency for rural development of Istria Ltd. Pazin; Agenzia per lo sviluppo rurale dell’Istria Pisino

Introduction

autochthonous cattle breeds in Croatia

number of animals drastically declined → systematic upgrading

1998. – 138 animals; 2008 – 460 animals

institutional measures (subsidies, promotions), established association (AZRRI), economic reaffirmations (brand “beef” production)

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

Aim

– analyze of pedigree and genetic variability within Istrian Cattle →

shaping breeding programmes and preserving strategies

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

Geographical location of Istrian Cattle populations

Material & Methods

Pedigree information

• data 2 151 (1994 – 2008.)

• ENDOG v4.5 program

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

Blood samples

• 51 unrelated animals

• Fstat v2.9.3, GenePop 3.4., Bottleneck v1.2.02 program

30 microsatellites belong to the panel of the MoDAD marker sets

recommended by FAO

Generation interval Number of herds

Pathway Mean SE Actual Effective

Father – son 5.803 0.396

HS 48 12.57

Father – daughter 5.811 0.363

HSS 21 4.01

Mother – son 7.313 0.375

HSSS 9 2.10

Mother – daughter

7.222 0.389

HSSSS 5 1.47

Total 6.526 0.134

Results

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

Generation interval and number of herds contributing sires (HS), grand-sires (HSS), great-grandsires (HSSS) in Istrian cattle.

Number of alleles (MNA), observed and expected heterozygosity (Ho, HE), polymorphism

information content (PIC), fixation coefficient (FIS) at 30 microsatellites.

Mean0.071***0.6170.6560.5896.6

Media

0.1770.7370.7660.58811TGLA53

0.0790.8240.8420.78410TGLA227

0.0860.7250.7640.7065TGLA126

0.0530.7150.7460.6867TGLA122

0.1160.5130.5370.4716NCAM

-0.0720.6880.7250.7848MM12

0.1950.5350.6140.495INRA063

0.197**0.6010.6410.4716INRA037

0.0530.4280.4710.4514INRA035

0.010.4420.490.495INRA032

0.166**0.7980.8220.6678INRA023

0.1460.5840.6580.5693INRA005

0.0030.6030.6360.6279ILSTS006

0.2410.1220.130.0982ILSTS005

0.0340.6410.6630.6479HEL9

MarcatoreLocus

MNA HO HE PIC FIS

BM1818 7 0.765 0.708 0.673 -0.092

BM1824 4 0.529 0.571 0.479 0.082

BM2113 7 0.588 0.719 0.671 0.192**

CSRM60 9 0.745 0.787 0.759 0.063

CSSM66 10 0.529 0.557 0.540 0.041

ETH10 5 0.490 0.478 0.430 -0.015

ETH152 6 0.863 0.771 0.738 -0.110

ETH185 7 0.804 0.778 0.749 -0.065

ETH225 7 0.333 0.791 0.761 0.275*

ETH3 7 0.647 0.597 0.557 -0.074

HAUT24 6 0.569 0.651 0.600 0.136

HAUT27 6 0.353 0.605 0.579 0.025

HEL1 6 0.627 0.747 0.707 0.136*

HEL13 6 0.490 0.640 0.571 0.229

HEL5 8 0.804 0.780 0.750 -0.085

MarcatoreLocus

MNA HO HE PIC FIS

Estimates of average inbreeding and the average relatedness in Istrian cattle

Complete equivalent

generations

Average F (%) in the whole

pedigree

Average relatedness

(AR) (%)

Inbred animals (%)

Average F (%) of inbred animals

1.78 2.38 3.58 8.71 16.03

low pedigree completeness

mating between relatives

high degree of relationships

and migration

weaker pedigree completeness, more precision mating scheme

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

absence of bottleneck using Wilcoxon test (TPM)

L-shaped mode shift grah showing lack of bottleneck in Istrian cattle population

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Allele frequencies class

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Conclusions

Sulle tracce delle Podoliche – On the Tracks of Grey Podolic cattle

Istrian cattle → did preserve part of genetic diversity

presence of inbreeding, with absence of bottleneck

unsystematic pedigree data → DNA testing

still endangered breed →

– continuous monitoring of population parameters,

– adjusting breeding program

preserving Istrian cattle breeds have to have high national and international priority

We thank all breeders and Reproduction Center for providing samples as well as Croatian Agriculture Agency.

The study of Croatian breeds was partly supported by the Ministry of Science, Education and Sports, Croatia.

AcknowledgementsAcknowledgements::