pdt-3rd chap(cell strct n funtn of organelles)

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    At the end of this topic, students should beable to :

    Learning outcomes :

    Identify the structure and state the function of :

    Cell wall

    nucleus

    Nucleolus

    mitochondria

    Rough and smooth endoplasmic reticulum

    Golgi bodyribosome

    s

    lysosomes

    Chloroplasts

    Centrioles

    describe the structure and properties of cellmembrane according to Fluid Mosaic Model

    4

    Key Terms

    selectively permeable

    Mitochondria

    Cell wall

    Ribosome

    Nucleus

    Nucleolus

    Golg body

    Lysosomes

    Chloroplast

    7

    CELL MEMBRANE

    A cell is separated from its environment bya plasma membrane which is selectively

    permeable.

    The plasma membrane is commonlydescribed as a FLUID MOSAIC MODEL.

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    Phospholipidbilayer

    Structure of cell membrane

    FLUID

    - Refers to phospholipid molecules that movefreely, so the plasma membrane is quite fluidand flexible.

    MOSAIC

    - Refers to the proteins that are embedded in

    the membrane

    Consist of

    - phospholipids bilayer - Glycoprotein

    - Glycolipid - Cholesterol

    - Protein

    FLUID MOSAIC MODEL

    Cell Wall

    Structure :

    Found only in plant cell

    surrounds the plasma membrane

    Made up of cellulose

    Function :

    Maintains the shape of the cell

    Protects the cell

    Cell Wall

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    Nucleus

    Structure :

    one in each cell

    Shape : Round or oval

    surrounded by nuclear envelope

    consists of nucleoplasm that contains

    Chromatin

    Nucleolus

    Function :

    Acts as the centre to control cell activities

    Nucleus

    Nucleolus

    Structure : Found within the nucleus Shape : Round or oval

    Function : Synthesise rRNA

    Nucleolus

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    Mitochondria

    Mitochondria

    Structure :

    Found in all eukaryotic cells

    Double membrane organelle :

    Inner membrane

    Cristae (foldings of inner membrane)

    Outer membrane

    Contains semifluid matrix

    Function :

    sites of cellular respiration generating ATP

    A membranous system which is continuouswith the outer nuclear membrane

    Two types of ER that differ in structure andfunction

    Rough endoplasmic reticulum (RER)

    Smooth endoplasmic reticulum (SER)

    Rough and Smooth EndoplasmicReticulum

    Rough ER (RER) has ribosomes attached to it

    Rough Endoplasmic Reticulum

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    Smooth ER (SER) lacks ribosomes.

    Smooth Endoplasmic Reticulum

    Functions of EndoplasmicReticulum

    Rough ER

    Involves in intracellular transport of proteins

    Smooth ER

    Participates in the synthesis of lipids,phospholipids & steroids

    Participates in carbohydrate metabolism

    Detoxifies drugs and poisons

    Golgi Body Golgi body

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    Structure

    Stacks of flattened sacs

    Functions :The site of biochemicals synthesis:

    Formations of lysosomes

    Assembling glycoproteins bycombining carbohydrates and proteins

    Production of digestive enzymes

    Golgi Body

    Ribosomes

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    Structure :

    Without membrane

    Can be found :

    freely in cytoplasm

    Attached to RER

    each consisting of 2 subunits

    large subunit

    small subunit

    Function :

    the site of protein synthesis

    Ribosomes

    Structure :

    Small spherical vesicles.

    Contain a variety of hydrolytic enzymes which can digestmaterial within the cell

    Functions :

    Break down macromolecules such as proteins,polysaccharides and nucleic acids

    Digest old and worn out organelles (autophagy)

    Destruction of cells by their own lysosomes

    - important during metamorphosis and development

    Lysosomes

    Chloroplasts

    Structure :

    Surrounded by a double membrane

    Outer membrane Inner membrane

    Thylakoid membrane : part of inner

    membrane system

    Contain photosynthetic pigments

    Stroma : gel-like matrix

    Function :

    sites of photosynthesis.

    Chloroplasts

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    Centrioles

    1 set of tripletmicrotubules

    Structure :

    found in animals cells.

    Pair of cylindrical structures located in thecentrosome.

    Each cylinder consists of nine sets of tripletmicrotubules arranged in a ring.

    Functions :

    as organisers of spindle fibres during cell division

    produce basal bodies from which flagella and ciliadeveloped.

    Centrioles

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    Key Terms

    Selectively permeable

    Hypotonic

    Hypertonic

    Dynamic equilibrium

    Passive transport

    Active transport

    Passive Transport

    The diffusion of a substance

    across a biological membrane

    down concentration gradient

    without the supply of energy (ATP).

    TYPES OFPASSIVE

    TRANSPORT(Not require energy)

    Diffusion FacilitatedDiffusion

    Osmosis

    Definition:

    Movement of the substances (molecules, ions &atoms) from a region of higher concentration to a

    lower concentration.

    Movements of individual molecules are random

    DIFFUSION

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    The phospholipid bilayer is permeable to very small

    uncharged molecules like oxygen and carbondioxide.

    These molecules diffuse freely in and out of the

    cell through the phospholipid bilayer.

    The phospholipid bilayer is not permeable to charged

    ions

    DIFFUSION

    DIFFUSION

    e.g. a permeable membrane separating asolution with dye molecules from pure water,

    until both solutions have equal concentrations ofthe dye - dynamic equilibrium

    each substance diffuses down its own

    concentration gradient

    DIFFUSION of two solutes

    OSMOSIS

    Definition

    The passive movement of water molecules

    across a selectively permeable membrane from the hypotonic solution to the hypertonic

    solution

    The movement continues until both solutions are

    isotonic.

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    OSMOSIS

    OSMOSIS

    when the two solutions are isotonic :

    water molecules move at equal rates

    from one to the other

    with no net movement

    OSMOSIS

    The Concepts of Water Potential

    Definition :

    the physical property that predicts

    the direction in which water molecules will flow

    determined by solute concentrationsolute concentration and applied

    pressurepressure.

    OSMOSIS

    The Concepts of Water Potential

    The symbol : a Greek alphabet, psi, The units : kilopascalskilopascals (kPakPa) @ megapascals (MPa)

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    OSMOSIS

    Pure water has the highest water potential which isset at zero

    Dissolving solute molecules in pure water willreduce the water potential (more negative) as theconcentration of water molecules lower

    OSMOSIS The concept of water potential

    Morenegative

    LessNegative

    Water Potential,

    Solute Potential, s

    Pressure Potential, p

    OSMOSIS

    The Concepts of Water Potential

    The three quantities

    The combined effects of pressure and [solute] onwater potential are incorporated into the followingequation:

    = s + p *

    OSMOSIS

    The Concepts of Water Potential(in plant cell)

    p: due to the presence of cell wall

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    OSMOSIS

    Definition (the concept of water potential)

    The passive movement of water molecules

    across a selectively permeable membrane from the solution of higher water potential to the

    solution of lower water potential.

    FACILITATED DIFFUSION

    Definition :

    Diffusion of solutes across a membrane with thehelp of transport proteins.

    The movement is spontaneous.bound to specific transport proteinsdown their concentration gradients.without using energy

    1. Carrier protein

    MODES OF FACILITATED DIFFUSION

    2. Channel protein

    depends on the types of the transport proteins :

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    the movement of a substance across abiological membrane

    Active transport

    DEFINITION

    against its concentration gradient

    with the help of energy input andspecific transport protein

    to bind the carrier &the solute together

    to enable the carrier to

    translocate the soluteacross the membrane

    to separate the solutefrom the carrier

    Active transport

    The ATP is needed