pawitra pulbutr m.sc. in pharm (pharmacology)....
TRANSCRIPT
Pawitra PulbutrPawitra Pulbutr
M.Sc. In Pharm M.Sc. In Pharm (Pharmacology)(Pharmacology)
วั�ตถุ�ประสงค์�เชิ�งพฤต�กรรมวั�ตถุ�ประสงค์�เชิ�งพฤต�กรรม
• ม�ค์วัามเข้�าใจและอธิ�บายถุ งกลไกการออกฤทธิ�#และค์วัามเป$นพ�ษข้องสารเค์ม�ท�'เป$น Antiseptics และ Disinfectants
• ม�ค์วัามเข้�าใจและอธิ�บายถุ งข้�อดี�และข้�อเส�ยข้องสารเค์ม�ท�'เป$น Antiseptics และ Disinfectants
Sterilization การท*าให้�ปลอดีเชิ,-อ• destroy microorganism including spores
Disinfection การท*าลายการต�ดีเชิ,-อ• reduce or eradicate microorganism
WORDSWORDS
Disinfectants
• chemicals that inhibit or kill micoorganism
• use with non-living surface
Antiseptics
• chemicals that inhibit or kill microorganism
• less toxic
• use with living surface
Properties of good antiseptics & Properties of good antiseptics & disinfectantsdisinfectants
• active to all microorganisms
• rapid & long action
• water soluble & stable
• tolerate to soap & organic material
• non-toxic
• less systemic absorption, non-allergenic
• well penetrate
• no colored, good smell
• cheap
NO antiseptics & NO antiseptics & disinfectants!!disinfectants!!
Alcohol
• rapid evaporation…no residual action
• can not penetrate organic material
• may cause irritation & dryness
• USEUSE
• antiseptic before injection
• disinfectants
ChlorhexidineChlorhexidine
• has residual action
• tolerated to organic material
• - destroy by anionic & non ionicsurfactants
• less irritation to skin
• USE
• chlorhexidine digluconate 2%, 4%
• - pre operation … skin cleaning
• infant bathing
HalogenHalogen
• iodine
• chlorine
• oxidize sulhydryl (SH) into S-S
• denature protein
• protein dysfunction & precipitation
Iodine
• active to bacteria, fungi, protozoa, virus , spore
• tincture of iodine USP
• 2% iodine + 2.4 % sodium iodide in alc.
• Most potent antiseptics
• irritation & hypersensitivity
Iodophor
• cpx of iodine + surface active agents
• iodine + polyvinyl pyrrolidine
• = povidone iodine (betadine®)
• as active as iodine
• less irritation & hypersensitivity
• not staining
• USE
• wound antiseptic
ChlorineChlorine
• water soluble into hypochlorus, HOCL
• active to bacteria, virus, protozoa, fungi, spore
• destroy by organic material
Chlorophor..slow release HOCL
• sodium hypochlorite
• chloramine
Phenolic compoundsPhenolic compoundsPhenol
• toxic….NO longer use
• carcinogenesis
• hyperbilirubinemia in neonates
Phenolic agents
• o-phenylphenol, o-benzyl-p-chlorophenol, p-tertiary amylphenol
• mixture of phenolic derivative
• cresol, xylenol
Phenolic compoundsPhenolic compounds
• cell membrane / cell wall disturbance
• precipitation of protein
• inactivate enz.
• Active to bacteria, fungi, virus
• No effect to spore
• USE
• disinfectants to hard surface..in lab/hospital
• not use in nursery…hyperbilirubinemia
HexachloropheneHexachlorophene
• active to most gm+ bacteria
• hexachlorophene 3% in soap
• USE
• antiseptic in pre-operation
• infant bathing ….
• May accumulate..cause heart & brain abnormal
•...cerebral edema , convulsion in premature infants
Quaternary ammonium Quaternary ammonium compoundcompound
• cationic surface active detergents
• benzalkonium chloride
• cetylpyridine chloride
• benzethonium chloride
• inactivate enzyme
• denaturation of protein
• active to bacteria(+), fungi, lipophillic virus
• sporistatic
AldehydesAldehydes
• formaldehyde, glutaraldehyde
• alkylation protein / nucleic acid
• active to bacteria, fungi, virus, spore
• USE
• disinfectants in medical equipments
Hydrogen peroxideHydrogen peroxide
• hydrogen peroxide 3% & 6%
• USE
• disinfectants…respirator, soft contact lenses
• wound antiseptics…catalase/ peroxidase...O2
gas + H2
O
• vapor phase hydrogen peroxide (VPHP)
• USE
• gaseous sterilant…less toxic than ethylene o xide, formaldehyde
Release hydroxyl radical …cell membrane disturbance
• thiomerosal (merthiolate)
• -thiomerosal 0.001 0.004%… preservative in vaccine
• -tincture merthiolate 0.1%…pre operation antiseptics
• merbromin (mercurochrome) ยาแดีง
Mercury saltsMercury salts
Silver saltsSilver salts
• silver nitrate
• used as eye drop to prevent gonococcal ophthalmitis in newborns
• silver sulfadiazine
• burn wound