pathogenesis
DESCRIPTION
pathogenesis. KOCH’S POSTULATES. Diseased animal. Healthy animal. The Postulates:. Tools:. 1. The suspected pathogenic organism should be present in all cases of the disease and absent from healthy animals. Microscopy, staining. Red blood cell. Observe - PowerPoint PPT PresentationTRANSCRIPT
pathogenesis
Fig. 1-15KOCH’S POSTULATES
Tools:Diseasedanimal
Healthyanimal
Redbloodcell
Observeblood/tissueunder themicroscope
Redbloodcell
The Postulates:
1. The suspected pathogenic organism should be present in all cases of the disease and absent from healthy animals.
2. The suspected organism should be grown in pure culture.
Suspectedpathogen
Microscopy, staining
Noorganismspresent
Streak agar platewith sample from eitherdiseased orhealthy animalColonies of
suspectedpathogen
Laboratory culture
3. Cells from a pure culture of the suspected organism should cause disease in a healthy animal.
Experimental animal
Diseased animal
Inoculate healthy animal withcells of suspected pathogen
Remove blood or tissue sampleand observe by microscopy
Laboratoryculture
Suspectedpathogen
Laboratory reisolation4. The organism should be reisolated and shown to be the same as the original.
Pure culture(must besameorganismas before)
• Primary pathogens: Cause disease in healthy hosts• Opportunisitc pathogens: Cause disease only in
immunocompromised patients• Virulence is a measure of the severity of a disease.• - Infectious dose vs. lethal dose
Figure 25.5
Figure 25.6
Figure 25.11 Figure 25.13A
Table 28-3
Figure 25.14Figure 25.14
Bacteria can attach to surfaces in bulk, forming a biofilm.- Play important roles in chronic infections Figure 25.15A
Streptococcus pyogenes M proteinBordetella pertussis Pertactin
Figure 25.16
AB Toxins• B subunit: Binds to host cell• - Delivers A subunit to
cytoplasm• - Often five B subunits
form a pore for A entry.
Figure 25.17B
A subunit: Has toxic activity- ADP-ribosyltransferase- Diphtheria toxin- Cholera toxin
Figure 25.17A
Figure 13.7A
Figure 25.20
• Animation: Cholera Toxin Mode of Action
Click box to launch animation
ADP-Ribosylating Toxins
Alpha Toxin• The hemolytic alpha toxin is produced by
Staphylococcus aureus. • - Forms a transmembrane, seven-member pore
in target cell membranes
Figure 25.18
Anthrax Toxin
• Made by Bacillus anthracis
• Two active toxins:• - Edema factor raises
cAMP levels. • - Causes fluid
secretion, tissue swelling• - Lethal factor cleaves
protein kinases.• - Blocks immune
system from attacking
Figure 25.21C
Endotoxins• Made only by Gram-negative bacteria• Present in lipopolysaccharide of outer membrane• - Lipid A released as bacteria die• - Causes massive release of cytokine from host cells• - Can trigger fever, shock, and death
Figure 25.22
Figure 25.31
Figure 25.32
Figure 25.29