YMC GENERAL CATALOG

8Packing Materials

YMC-Exphere C18

YMC＊GEL HG series

Types and characteristics of packing materials

Spherical packing materials for preparative isolation

Irregular packing materials for preparative isolation

156,157

156,157

158,159

160～163

164

155

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High strength packing material

Exphere C18, YMC＊GEL HG series: High strength packing material

YMC-Exphere C18, YMC＊GEL HG seriesYMC-Exphere C18 and HG series are newly developed packing materials based on high density and high strength silica gel. Excellent mechanical stability allows them to be used for a long term repacking into the dynamic axial compression column. Moreover, Exphere C18 has new polymeric modification, so it shows excellent stability under acidic and alkaline conditions.

Features

● High density and high strength silica gel● Narrow distribution of particle size and pore size● Excellent chemical stability under acidic

conditions due to polymeric bonding● Excellent peak shapes

Exphere C18Newly developed silica gel

The silica gel of YMC-Exphere has perfect spherical shape and smooth surface. Therefore, it provides higher plate number.

Exphere C18 Competitor A

Excellent resolution

1

2

3

4

56

150 5 20 min10

Exphere C18 shows excellent peak separation for peptides below MW 10,000.

Column ： YMC-Exphere C18 (15 µm, 120 Å) 150×4.6 mmI.D.

Eluent ： A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 20-40% B (0-20 min)

Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 220 nm

1. Oxytocin2. Met-Enkephalin3. Leu-Enkephalin4. Neurotensin5. γ-Endorphin6. β-Endorphin

SpecificationParticle size ：10 µm, 15 µm, 20 µmPore size ：120 ÅCarbon content ：21％Available pH reange ：pH 1.0 〜 8.0

Mechanical stability (repacking)

MPa/m

Packing number

Competitor D (15 µm, 120 Å)Exphere C18 (15 µm, 120 Å)

5 1010

0.5

1.0

1.5

2.0

2.5

Column pressure

To evaluate mechanical strength for packed gel, repeated packing and unpacking using a DAC column was undertaken.SEM images of 1st and 13th repacking are shown in above.Exphere C18 and HG series use a high strength silica; therefore column pressure is the same as the initial value after 13 times repacking. High mechanical stability enables longer life time.

Packing conditionsColumn size ：100×50 mmI.D., Packing pressure : 6.5 MPa

Test conditionsEluent ：methanol/water (85/15), Flow rate : 50 mL/min

Exphere C18

Competitor D

After 1st packing

After 1st packing After 13th repacking

After 13th repacking

SEM images of packed gel

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Features

● High density and high strength silica gel● Narrow distribution of particle size and pore size● Same chemical modifications as YMC＊GEL series

YMC＊GEL HG series

Chemical stability

pH 1

0

20

40

60

80

100

120

0 20 40 60 80 100 120

Exphere C18 (15 µm, 120 Å)

Silica based monomeric C18(10 µm or 15 µm, 120 Å)

Rate of retention time (%)

Time (hr)

0

20

40

60

80

100

120

0 20 40 60 80 100 120

Time (hr)

Rate of plate number (%)

Silica gel basedmonomeric C18(10 µm or 15 µm, 120 Å)

Exphere C18 (15 µm, 120 Å)

Test conditions of low-pH stabilityThe colnmns were purged with acetonitrile/water/TFA (10/90/1) and stored at 70 ℃. The column performance was evaluated every 20 hrs.

Performance test conditionsColumn ：250×6.0 mmI.D.Eluent ：acetonitrile/water (60/40)Flow rate ：1.7 mL/minTemperature ：37 ℃Sample ：n-butyl benzoate

Exphere C18 shows excellent stability under acidic and alkaline conditions due to new polymeric modification to the gel. It is suitable for purification which uses acidic mobile phase and requires column wash with alkaline solution.

Ordering information

Packing material Particle size（µm）

Pore size（Å） Product number

ODS-A-HG 10

120

AAG12S1115 AAG12S1620 AAG12S2150 AAG12S5010

300AAG30S11

15 AAG30S1620 AAG30S21

ODS-AQ-HG 10

120

AQG12S1115 AQG12S1620 AQG12S2150 AQG12S5010

200AQG20S11

15 AQG20S1620 AQG20S21

C8-HG 10120

OCG12S1115 OCG12S1620 OCG12S2110

200OCG20S11

15 OCG20S1620 OCG20S21

C4-HG 10300

BUG30S1115 BUG30S1620 BUG30S21

Other than those listed above, please inquire us.

Exphere C18

Packing material Particle size（µm）

Pore size（Å） Product number

C18 10120

EA12S1115 EA12S1620 EA12S21

YMC＊GEL HG series

Packing material Particle size（µm）

Pore size（Å） Product number

SIL-HG 10

120

SLG12S1115 SLG12S1620 SLG12S2150 SLG12S5010

200SLG20S11

15 SLG20S1620 SLG20S2110

300SLG30S11

15 SLG30S1620 SLG30S21

YMC＊GEL HG series

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Packing materials

T y p e s a n d c h a r a c t e r i s t i c s o f p a c k i n g m a t e r i a l sYMC Co., Ltd. offers both spherical and irregular types of packing materials. These products come in a wide range of particle sizes.Spherical packing materials are used for both analytical and preparative columns. Since the packing homogeneity of spherical packing materials is generally higher than that of irregular packing materials, spherical packing materials are characterized by high separability, excellent packing reproducibility and small pressure loss. However, spherical packing materials are more expensive than irregular packing materials.

Irregular packing materials are mainly used for preparative chromatography and often also for open column chromatography. Irregular packing materials are significantly less expensive than spherical packing materials.For both spherical and irregular packing materials, smaller particle sizes result in higher separability, but with higher column backpressure and at higher prices.We recommend that users consider the tradeoff between required separability and the consumption and cost of columns when selecting particle shapes and particle sizes of packing materials.

YMC＊GEL

S p h e r i c a l p a c k i n g m a t e r i a l s f o r p r e p a r a t i v e i s o l a t i o nSpherical packing materials for preparative isolation come in many types of bonded phases and bare silica gel. A wide range of particle sizes are available up to as large as 150 µm.The quality of spherical packing materials is strictly controlled, similar to YMC-Pack.YMC S-50, S-75 and S-150 gels, with narrow particle size distribution and high performance, are useful for large-scale isolation.

I r r e g u l a r p a c k i n g m a t e r i a l s f o r p r e p a r a t i v e i s o l a t i o nIrregular packing materials are of bare silica gel only.Irregular packing materials are widely applicable, from semi-preparative isolation to industrial preparation.Irregular packing materials are reproducible with minimal lot-to-lot variation.

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Product name Pore size（Å）

Particle size（µm） Product number

YMC＊GEL ODS-A 1203 AA12S035 AA12S0510 AA12S11

300 5 AA30S05

YMC＊GEL ODS-AM 120 3 AM12S035 AM12S05

YMC＊GEL ODS-AQ 1203 AQ12S035 AQ12S0510 AQ12S11

YMC＊GEL TMS 120 5 TM12S05

YMC＊GEL C8120 5 OC12S05300 5 OC30S05

YMC＊GEL Ph 120 5 PH12S05

YMC＊GEL CN 120 5 CN12S05300 5 CN30S05

YMC＊GEL NH2 120 5 NH12S05

YMC＊GEL C4120 5 BU12S05300 5 BU30S05

YMC＊GEL PolyamineⅡ 120 5 PB12S05YMC＊GEL PolymerC18 6 PC99S06

YMC＊GEL SIL60 5 SL06S05

10 SL06S11

120 5 SL12S0510 SL12S11

Spherical packing materials for analytical separation Spherical

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Packing materials

S p h e r i c a l p a c k i n g m a t e r i a l s f o r p r e p a r a t i v e i s o l a t i o nSpherical packing materials for preparative separation come in a wide variety of phases including reversed-phase, normal-phase, and chiral stationary phase, and are applicable to a wide range of fields in preparative separation. These products come in six particle sizes: 10 µm, 15 µm, 20 µm, 50 µm, 75 µm and 150 µm. The quality of all sizes of products is strictly controlled.YMC gels with particle sizes of 20 µm, 50 µm, 75 µm

and 150 µm have narrow particle size distributions and excellent cost performance. Among these, YMC S-20 with an appropriate particle size of 20 µm is useful for semi-preparative separation and separation on axial compression column as well as industrial separation.YMC*GEL is used all around the world for its wide product range in phase and particle size.

YMC＊GEL

Product Characteristics USPCLASS No.

Particle size（µm）

Pore size（Å） C% pH range

Exphere C18 High strength and high chemical stability packing materialNarrow particle distribution and pore size L1 10, 15, 20 120 21 1.0 〜 8.0

HG series* High strength packing material used with conventional modificationSame separation pattern as YMC＊GEL － － － － －

Pro C18ODS packing materials highly endcapped ever to minimize the effect of residual silanols. It offers excellent separation performance for most of the compounds such as basic compounds.

L1 10 120 16 2.0 〜 8.0

ODS-A

The most popular product among ODS packing materials. The highly endcapped surface structure with moderate carbon content offers excellent separation performance for a wide range of compounds. It is suitable as a first choice.

L1

10, 15, 20, 50, 75, 150 120 17

2.0 〜 7.510, 15, 20 200 12

10, 15, 20 300 7

ODS-AQSeparation characteristics for hydrophilic compounds are more distinct than with ODS-A. Retention behavior is different from ODS-A for separation using eluents with relatively high water content.

L110, 15, 20, 50 120 14

2.0 〜 7.510, 15 200 10

PolymerC18 Packing material with an n-octadecyl group incorporated into the methacrylate polymer, characterized by the broad usable pH range. － 10 － C18=10% 1.5 〜 13.0

C8（Octyl）Reversed-phase packing material with an n-octyl group, fully endcapped, suitable for relatively highly hydrophobic compounds with too strong retention on ODS.

L710, 15, 20 120 10

2.0 〜 7.510, 15, 20 200 710, 20 300 4

Ph（Phenyl） Reversed-phase packing material with a phenyl group. The π electron interaction gives a separation selectivity different from ODS. L11 10, 15, 20 120 9 2.0 〜 7.5

C4（Butyl）

Reversed-phase packing material with an n-butyl group, fully endcapped. This packing material has a less hydrophobic surface structure than C8 packing material, making it suitable for separation of proteins.

L26

10, 15, 20 120 7

2.0 〜 7.510, 15, 20 200 5

10, 15, 20 300 3

C1（TMS）Reversed-phase packing material with a methyl group, allowing rapid elution compared to other packing materials for retention based on hydrophobic interaction.

L13 10, 15, 20 120 4 2.0 〜 7.5

NH2 Normal-phase packing material with an aminopropyl group. L8 10 120 3 2.0 〜 7.5

CN（Cyano）Normal/reversed-phase packing material with a cyanopropyl group. The medium polarity of the functional group allows selectable normal-phase and reversed-phase separation modes.

L10 10, 15, 20 120 7 2.0 〜 7.5

basic

Reversed-phase packing material designed for separation of basic compounds, including pharmaceutical products. This packing material has a lower hydrophobicity than conventional ODS, making it suitable for separation of peptides with molecular weight of several thousands.

L7 10 200 7 2.0 〜 7.5

Diol Packing material with dihydroxypropyl, useful for gel filtration or normal-phase applications L20

10, 15 120 －2.0 〜 7.5

15 200 －

Silica

Totally porous silica gel packing material, popular among normal-phase products. Spherical packing materials with large particle sizes are available for applications at low-middle pressure and open columns.

L3

10, 15, 75, 150 60 －

2.0 〜 7.510, 15, 20, 50, 75, 150 120 －

10, 15 200 －10, 15 300 －10, 50 1000 －

PROTEIN-RP Silica-based reversed-phase packing material designed for separation of proteins and peptides, characterized by excellent acid resistance. － 20 － 4 1.5 〜 7.5

PREP CD ST Suitable for preparative isolation of optical isomers. ST is generally suitable for reversed-phase applications and PM is useful for normal-phase and reversed-phase applications.

L45 10, 20, 50 120 － 2.0 〜 7.0PREP CD PM

* The details are referred to original YMC＊GEL (ODS-A, ODS-AQ, C8, C4), respectively.

YMC＊GEL packing materials for preparative isolation

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Product name Particle size distribution （µm） Mean pore size （Å） Specific surface area （m2/g） Pore volume （mL/g）SL06S75 42-105 65±10 700±50 1.15±0.10SL12S75 42-105 120±10 400±50 1.10±0.10SL06SA5 105-210 65±10 700±50 1.15±0.10SL12SA5 105-210 120±10 400±50 1.10±0.10

pH (5% slurry), 6.0±1.0 Water content, ≦2%

Physical properties of spherical silica gel

High column efficiency can be obtained even when tapping method is used for packing

0 10 20 30 40 (min)

1

2

3

4

INJ.

tapping method

Theoretical plate numberColumn Pressure

N（4）＝2,500 0.5 MPa

Column ： ODS-A (S-50 µm, 120 Å) 500×20 mmI.D.

Eluent ：methanol/water (75/25)Flow rate ：10 mL/minDetection ：UV at 254 nm, 0.32 AUFSSample ： 1. Uracil

2. Methyl benzoate 3. Toluene 4. Naphthalene

Injection ：16 µL

0 10 20 30 40 (min)

1

2

3

4

slurry method

Theoretical plate numberColumn Pressure

N（4）＝2,7000.5 MPa

Comparison between the tapping method and the slurry method for packing

YMC S-50 gel was packed into columns using the tapping method and the slurry method, and the column performance was compared. It is demonstrated that the same column efficiency can be obtained using the easy tapping method as the slurry method.

Higher theoretical plate number is achieved as compared with irregular packing materials

0 10 20 30 (min)

43

2

1

INJ.

Theoretical plate number N（4）＝2,500

ODS-A（S-5O µm, 120 Å）

Column ：500×20 mmI.D.Eluent ：methanol/water (85/15)Flow rate ：40 mL/minDetection ：UV at 254 nm, 0.32 AUFSSample ： 1. Uracil

2. Methyl benzoate 3. Toluene 4. Naphthalene

Injection ：100 µL0 10 20 30 40

(min)

43

2

1

N（4）＝1,500

ODS（I-40/63 µm, 120 Å）

Comparison with a irregular packing material

An irregular packing material with a particle size of 40/60 µm and YMC S-50 gel were separately packed in individual columns using the tapping method, and their column performance was compared. It is demonstrated that YMC S-50 gel, a spherical packing material with a narrow particle size distribution, exhibits higher column efficiency.

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Packing materials

YMC＊GEL

Applicable to various size columns

Column Size:500×6.0 mmI.D.×2（Coupled Column）

inj.

20 40 (min)

1

2

Theoretical platenumber ： N (2)＝4,800Flow rate ： 0.7 mL/minInjection ： 1.8 µL

YMC S-50 gel was packed in various size preparative columns and their column efficiencies were compared. To ensure a realistic comparison, the mobile phase flow rate and sample injection volume were changed according to the ratios of column cross-sections.It is demonstrated that YMC S-50 gel can achieve high column efficiency in various size columns.

1000×20 mmI.D.Column Size:

inj.

20 40 (min)

1

2

1000×200 mmI.D.Column Size:

inj.

20 40 (min)

1 2

Comparison among column sizes

Column ： YMC-Pack ODS-AQ (S-50 µm, 120 Å)

Eluent ：methanol/water (85/15)Detection ：UV at 254 nm, 0.16 AUFSSample ： 1. Methyl benzoate

2. Toluene

N (2) ＝4,800 8.0 mL/min 20 µL

N (2) ＝4,300 800 mL/min 2.0 mL

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Spherical packing materials for preparative isolation

Packing material Particle size（µm）

Pore size（Å） Product number

Pro C18 10 120 AS12S11

ODS-A 10 120 AA12S11200 AA20S11300 AA30S11

15 120 AA12S16200 AA20S16300 AA30S16

20 120 AA12S21200 AA20S21300 AA30S21

50 120 AA12S5075 120 AA12S75150 120 AA12SA5

ODS-AQ 10 120 AQ12S11200 AQ20S11

15 120 AQ12S16200 AQ20S16

20 120 AQ12S2150 120 AQ12S50

PolymerC18 10 － PC99S10

C8 10 120 OC12S11200 OC20S11300 OC30S11

15 120 OC12S16200 OC20S16

20 120 OC12S21200 OC20S21300 OC30S21

Ph 10 120 PH12S1115 120 PH12S1620 120 PH12S21

C4 10 120 BU12S11200 BU20S11300 BU30S11

15 120 BU12S16200 BU20S16300 BU30S16

20 120 BU12S21200 BU20S21300 BU30S21

Other than those listed above, please inquire us.

Packing material Particle size（µm）

Pore size（Å） Product number

C1(TMS) 10 120 TM12S1115 120 TM12S1620 120 TM12S21

NH2 10 120 NH12S11

CN 10 120 CN12S1115 120 CN12S1620 120 CN12S21

basic 10 － BA99S11

Diol 10 120 DL12S1115 120 DL12S16

200 DL20S16

SIL 10 60 SL06S11120 SL12S11200 SL20S11300 SL30S111000 SLA0S11

15 60 SL06S16120 SL12S16200 SL20S16300 SL30S16

20 120 SL12S2150 120 SL12S50

1000 SLA0S5075 60 SL06S75

120 SL12S75150 60 SL06SA5

120 SL12SA5

PROTEIN-RP 20 － PR99S21

PREP CD ST 10 120 ST12S1120 120 ST12S2150 120 ST12S50

PREP CD PM 10 120 PM12S1120 120 PM12S2150 120 PM12S50

Spherical packing materials for preparative isolation Spherical

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Packing materials

I r r e g u l a r p a c k i n g m a t e r i a l s f o r p r e p a r a t i v e i s o l a t i o nIrregular packing materials for preparative isolation come in a wide variety of particle sizes and are applicable to a wide range of isolations from semi-preparative isolation to industrial preparation.YMC Co., Ltd.’s irregular packing materials for preparative isolation have excellent cost

performance and are applicable not only to open column chromatography but also to pretreatment of samples (partial purification and batch treatment for concentration). Among them, silica gel with a particle size of 63 - 210 µm is especially suitable for open column chromatography. Physical properties of an irregular silica gel are described below.

YMC ＊ GEL

Packing material Mean pore size （Å） Specific surface area （m2/g） Pore volume （mL/g） Apparent specific gravityYMC＊GEL SIL 65±10 450±50 0.7±0.1 0.43-0.53

Physical properties of an irregular silica gel

Particle sizes

Mesh size Packing material particle size （µm） Mean particle size （µm） Particle size distribution （D40/D90）230/70 63-210 95±10 ≦2.0400/230 40-63 53±5 ≦1.45500/400 25-40 33±3 ≦1.6

Water content……≦２％Water content is an important factor which determines the activity of silica gel. Greater water content results in lower activity and retention. The water content of YMC Co., Ltd.’s irregular silica gel is generally controlled to be 2% or less; however, products with water content of 5% and 2% or less

are both available for SIL-60-230/70 (60 Å, 63-210 µm) and SIL-60-400/230 (60 Å, 40-63 µm). Silica gel with water content of 5% is useful for samples having too strong of a retention on silica gel with water content of 2% or less.

Dry type (water content of 2% or less)

Packing material Pore size（Å）

Particle size（µm） Product number

YMC＊GEL SIL 6063-210 SL06IA440-63 SL06I5225-40 SL06I32

Wet type (water content of 5% )

Packing material Pore size（Å）

Particle size（µm） Product number

YMC＊GEL SIL 60 63-210 SL06IA4W40-63 SL06I52W

Irregular packing materials for preparative isolation Irregular

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