osm v.s. ism1-ba m. engin enginsu m.d., ph.d. kadıköyŞifa hastanesi İstanbul turkey
TRANSCRIPT
OSM v.s. ISM1-BA
M. Engin Enginsu M.D., Ph.D.
KadıköyŞifa Hastanesi
İstanbul Turkey
• KŞ Hospital IVF unit is a private clinic
• 600 cycles per year
• ISM 1 – BA is the only culutre medium used in the clinic for over 3 years
• The average age of the patients is 35,98
• >40 is %32,6
Protocols ISM 1 – BAOocyte collection in Universal IVF
untill ICSI
Transfer to ISM1 untill D2
Change to BA at 42-48 hrs
Blast developmentChange BA on 72-78 hrs never disturb on D4
Transfer in BA
OSMOocyte collection in OS Fert untill
ICSI
Transfer to OS Clev after ICSI
Refresh Os Clev on 22-26 hrs
Change to OM Blas on 72-78 hrs or ET
Blast developmentnever disturb on D4
Transfer in OS Blas
Day 2 Embryos
ISM1 BA OSM
Day 3 Embryos
ISM1 BA OSM
Blastocysts
ISM1 BA OSM
0
100
200
300
400
500
600
700
800
OSM %76 ISM1 BA %64
Oocyte
M2
Overall Results
n = 76 n = 114
Overall Results
66 65
97 98
7068
94 89
65 64
0102030405060708090
100
FR CR GR1 D2 D2-D3 R GR1 D3
OSM
ISM1 BA
Overall Results
47
28
0
10
20
30
40
50
PREGNANCY RATE
OSM
ISM1 BA
0
100
200
300
400
500
600
700
800
OSM %76 ISM1 BA %77
Oocyte
M2
Good Prognosis Group<40 yrs >3 oocytes
Good Prognosis Group<40 yrs >3 oocytes
68 68
96 97
7268
98 93
67 65
0102030405060708090
100
FR CR GR1 D2 D2-D3 R GR1 D3
OSM
ISM1 BA
Good Prognosis Group<40 yrs >3 oocytes
60
39
0
10
20
30
40
50
60
PREGNANCY RATE
OSM
ISM1 BA
Blastocyst Results
7367
99100
6872
9486
647073
5363
68
0102030405060708090
100
FR CR GR1D2
D2-D3R
GR1D3
D3-D5R
G1 BLOSMISM1 BA
Blastocyst Results
85
36
0
10
20
30
40
50
60
70
80
90
PREGNANCY RATE
OSM
ISM1 BA
THAW ET
38
23
0
5
10
15
20
25
30
35
40
PREGNANCY RATE
OSM
ISM1 BA
Conclusion
• On basis of fertilization, cleavage and embryo development rate OSM and ISM1-BA are comparable
• Blastocyts development is significantly better in OSM
• Clinical pregnancy rates are significantly better in OSM
• This may be the result of longer exposure of the embryos to Hyaluronan