Oriental Aquamarine Biotech India (P) LimitedCoimbatore

Nitrifying Bioreactor Technology for the Establishment of Biosecure

Recirculating Aquaculture Systems

Aquaculture

Conventional SystemsAquaculture Tanks

Ammonia & Nitrites

Excess FeedFecal Matter

Ocean/Waterbody

Conventional systems – problemsNeeds large quantities of water

Conventional systems -problemReduced survival rate

Conventional systems-problemsCauses water pollution

Oriental Aquamarine Biotech India Private Ltd - Coimbatore

• Technology developed by National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Kerala, India

• Technology commercialized by Oriental Aquamarine Biotech India Private Limited, Coimbatore, Tamil Nadu

The Solution – Bacterial ConsortiumA consortium of nitrifying bacteria have been identified which break down the ammonia and nitrites

Consortium grows as biofilm on beads packed and remove Nitrites & Ammonia inside a bioreactor

The solution-Nitrifying Bioreactor

• PBBR • SBSBR

Nitrifying Bioreactor

Stringed Bed Suspended Bio Reactor (SBSBR) -500 liters/day

Packed Bed Bio Reactor (PBBR)-60,000 liters/day

NH4+ < 0.1 ppm

NO2- < 0.5 ppm

NO3 10 – 100 ppm

Vibrio < 2 * 102 / ml

Luminescent Vibrio Nil

pH 7.5 – 8.5

No water exchange

Reef Quality Water.

Water quality parameters in the larval rearing system and treated water stabilized as under :

PROGRESS OF NITRIFICATION IN PBBR INTEGRATED PENAEUS MONODON MATURATION SYSTEM

0

1

2

3

4

5

6

7

8

1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70

Days

Con

cent

ratio

n (m

g/L)

TAN NO2-N NO3-N

NITRIFICATION EFFICIENCY

0

0.1

0.2

0.3

0.4

0.5

0.6

Co

nce

ntr

atio

n (

mg

/L)

1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70

Days

TAN-in TAN-out

0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

Days

Con

cent

ratio

n (m

g/L

)

Ammonia Input Ammonia Output

NITRIFICATION EFFICIENCY

0

0.1

0.2

0.3

0.4

0.5

0.6

Con

cent

ratio

n (m

g/L)

1 4 7 10 13 16 19 22 25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70

Days

NO2-N-in NO2-N-0ut

2

Substrate reduction at varied concentration

VOLUMETRIC TAN REMOVAL RATES BY THE REACTOR WITH FLOW RATE

0

20

40

60

80

100

120

140

250 750 1500 2500

Flow rates (L/hr)

TA

N r

emov

al (

g/m

3/da

y)

TAN removal Rates

TAN, NO2-N and NO3-N experimental systems integrated with SBSBR rearingPenaeus mondon

0

1

2

3

4

5

6

7

8

9

10

0 5 10 15 20 25 30

Days

Conc

entra

tion (

mg/L)

TAN NO2-N NO3-N

Stringed Bed Suspended Bioreactor Deployed in a Larval rearing system at Queens Hatchery, Kodungalloore

TNFDC, Aliyar (0-PPT Trials)Fish: Koicarp (10grms)

0

50

100

150

200

250

300

1 5 10 15 20

Days

Fis

h Q

TY

Golden fish farm ,Calicut (0-PPT Trials)Fish: Angel (Size:1cm to 3cm)

-100

100

300

500

700

900

1100

1300

1 5 10 15 20

Days

Fis

h Q

TY

CMFRI -Kochi

0 5 10 15 20 25 30

-2

-1

0

1

2

3

4

5

6

7

CMFRI PERFORMANCE CHART

NH3

NO2

NO3

No of days

ppm

con

cent

rati

on

• Patent in India :

• Patent Application # 828/DEL/2000

• International Patent :

• International patent classification: C02F 3/13.

• International application No. PCT/IN00/00097

• Technology is also patented in South East Asian Countries with a well-developed aquaculture industry such as

Thailand

Japan

Philippines

Indonesia and

South Korea.

Publication

APPLICATION

• Ornamental fish FRESH WATER

MARINE WATER

• Edible fishSEA BASS

LOBSTER

SHIRMP

PRAWN

CRAB

COBIA etc

Nitrifying Bioreactor - Advantages• Improved quality of fish produced (Organic)• Increase in fish survival rate• Increase in rate of growth and the size of fish• Better resistance to diseases• Reduction in production costs• Improvement in surrounding environment

Nitrifying Bioreactor - Technology• Patented technology• First of its kind in the world• Maintains reef-like conditions in aquaculture tanks• Can be used for any level of salinity

Fresh Water Salt Water Brackish Water

• Can be used to farm a variety of marine animals Shrimp Ornamental Fish Crabs/Lobsters

BENEFITS

Maturation(Developing ovary in the animals reared in the recirculating system)

Brood-stock Maintenance and Rearing

Increased stocking & survival rate

RECIRCULATING AQUACULTURE SYSTEM

Location flexible – transport costs minimized

Fewer tanks – less capital outlay

Temp and other parameters controlled

Planned production schedules, control supplies of fingerlings to target seasonal market demand by cage farmers Lower mortality, improved FCRs

RAS allow diversification of the farmed species base for the market substituting seafood imports and opening new markets in live fish sales

Thank you

Mohan KandaswamyDirector

Oriental Aquamarine Biotech India P LtdU-7 Kovaipudur, Coimbatore - 641042

Mobile : 91 94429 51725E-mail: mohan@nitrifying-bioreactor.com