organizers school of pharmacy, faculty of medicine,the...

for Introducing Pharmacy Research Postgraduate Programmes amp HKPFS SchemeCUM

Innovating Pharmaceutical Sciences Advancing Health Care

4 ndash 5 JUL 2017Tue ndash Wed 4 July ( Workshop ) 930 AM ndash 500 PM 5 July ( Symposium ) 830 AM ndash 530 PM

CUHK HONG KONGLecture Theater G02 Lo Kwee-Seong Integrated Biomedical Sciences Building Area 39The Chinese University of Hong Kong Shatin Hong Kong

12th PharmSciAsia Symposium

CUHK SUMMER WORKSHOP

ORGANIZERSSchool of Pharmacy Faculty of Medicine The Chinese University of Hong KongDepartment of Pharmacy Faculty of Science National University of SingaporeAmerican Association of Pharmaceutical Scientists ndash NUS and CUHK Student Chapters

Day 1CUHK SUMMER WORKSHOP

for Introducing Pharmacy Research Postgraduate Programmes amp HKPFS Scheme~middot ~ Pursuing A Rewarding Career In Research ~middot ~

4 July 2017 (Tuesday) ︳0930AM ndash 500PMLecture Theatre G02 Lo Kwee-Seong Integrated Biomedical Sciences Building (LKIBSB) Area 39 CUHK

Organized by School of Pharmacy Faculty of Medicine The Chinese University of Hong Kong

Date Time Event Presenter4 July 2017 (Tuesday)

0930 ndash 1000 Registration1000 ndash 1005 Opening Remarks Prof Joan ZUO

Director and Professor School of Pharmacy CUHK

1005 ndash 1100 Launching Your Research CareerHighlight - How to develop a career as a researcher - Career opportunities in the research field

Prof Vincent LEEResearch ProfessorSchool of Pharmacy CUHK

1100 ndash 1110 Group Photo Session amp Break

1110 ndash 1210 Pharmacy Research at CUHK CUHK Pharmacy Professors

1210 ndash 1400 Lunch with CUHK Pharmacy Professors and Current Pharmacy Postgraduate Students Highlight - Experience sharing and discussion

CUHK Pharmacy Professors amp Current Pharmacy Postgraduate Students

1415 ndash 1515 Study at CUHKHighlight - Introducing the School of Pharmacy - Introducing Research Postgraduate Programmes- HKPFS studentships travel grants amp scholarships- Overseas Conferences - Admission procedure

Prof Joan ZUODirector and ProfessorSchool of Pharmacy CUHK

1515 ndash 1530 AAPS-CUHK Students Chapter Presentation Ms Cathy ZHANGChairpersonAAPS-CUHK Student Chapter

1530 ndash 1545 Closing AddressSouvenir Presentation


1545 ndash 1700 School Lab Tour

Symposium Preparation

Current Pharmacy Postgraduate Students

Symposiumrsquos Poster Participants

1600 1630 1700

Shuttle Bus Time Schedule

Area 39 MTR Station

Day 212th PharmSciAsia Symposium

~middot ~ Innovating Pharmaceutical Sciences Advancing Health Care ~middot ~5 July 2017 (Wednesday) ︳0830AM ndash 535PM

Lecture Theatre G02 Lo Kwee-Seong Integrated Biomedical Sciences Building (LKIBSB) Area 39 CUHKOrganized by

Department of Pharmacy Faculty of Science National University of SingaporeAmerican Association of Pharmaceutical Scientists ndash NUS and CUHK Student Chapters

Date Time Event Presenter5 July 2017 (Wednesday)

0830 ndash 0900 Registration0900 ndash 0910 Welcome Address Prof Joan ZUO

Director and ProfessorSchool of Pharmacy CUHK

0910 ndash 0920 Opening Address Dr Pei Shi ONG Lecturer Department of Pharmacy NUSFaculty Advisor AAPS-NUS Student Chapter

0920 ndash 0930 Group Photo Session

0930 ndash 1000Faculty Speaker 1Naturally occurring stilbenes beyond resveratrol superior anti-inflammatory potencies and favorable pharmacokinetics

Prof Haishu LIN Assistant ProfessorDepartment of Pharmacy NUS

1000 ndash 1045 Student Speakers 1 ndash 31 Translational Pharmacokinetic Model for the Predictions of DB213

Nose-to-brain Transport2 An in vitro herb-drug interaction approach on HPLC fingerprint

comparison in liver microsomes and its validation in rats using ShensongYangxin Capsule and Verapamil

3 Towards the development of novel inhibitors for Chikungunya virus infection

1 Ms Qianwen WANGSchool of Pharmacy CUHK2 Mr Qiao ZHOUChina Pharmaceutical University3 Ms Quy Thi Ngoc TRANDepartment of Pharmacy NUS

1045 ndash 1115 Coffee Break and Poster Viewing1115 ndash 1145 Faculty Speaker 2

The Impact of Pharmacy Outreach on Atrial Fibrillation Detection Knowledge and Medication Adherence in Hong Kong Elderly

Prof Vivian LEE Associate ProfessorSchool of Pharmacy CUHKAssistant Dean (Student Development)Faculty of Medicine CUHK

1145 ndash 1230 Student Speakers 4 ndash 64 Prediction of subject classification performance of mixture model in

NONMEM5 Effect of Microneedle Geometry and Body Curvatures on Skin

Penetration for the Development of a 3D-Printed Personalised Microneedle Eye Patch

6 Adipocytes-derived Collagen Reorganization in Microenvironment Promote Breast Cancer Metastasis

4 Mr Ka Ho HUISchool of Pharmacy CUHK5 Mr Seng Han LIMDepartment of Pharmacy NUS6 Ms Xiaohui WEIChina Pharmaceutical University

1230 ndash 1300 Faculty Speaker 3Paying for Stratified Medicine Seeking A Delicate Balance

Prof Hwee-Lin WEE Assistant ProfessorDepartment of Pharmacy NUS

1300 ndash 1415 Lunch and Poster Viewing1415 ndash 1445 Faculty Speaker 4

Drug Delivery for Proliferative VitreoretinopathyProf Thomas LEE Assistant ProfessorSchool of Pharmacy CUHK

1445 ndash 1515 Student Speakers 7 ndash 87 Excipient Selection in Tableting The Importance of Making a

Judicious Choice8 Oligonucleotides-conjugated Poly(ethylene oxide)-block-

poly(amp949-caprolactone) (PEG-b-PCL) Nanoparticles as Drug Carriers Targeting Scavenger Receptor Class A

7 Ms Tze Ning HIEWDepartment of Pharmacy NUS

8 Mr Ho Yin LISchool of Pharmacy CUHK

1515 ndash 1545 Faculty Speaker 5Combinatorial Therapy Incorporating Histone Deacetylase Inhibitor (HDACI) For More Effective Cancer Treatment

Dr Pei Shi ONG LecturerDepartment of Pharmacy NUS

1545 ndash 1615 Afternoon Tea and Poster Viewing1615 ndash 1645 Student Speakers 9 ndash 10

9 Increased FAM3C Expression Promotes Proliferation and Metastasis of Lung Cancer Cells through Activation of RalGDSRalA Signaling Pathway

10 Cooperative down-regulation of ribosomal protein L10 and NF-κB signaling pathway is responsible for the anti-proliferative effects by DMAPT in pancreatic cancer cells

9 Mr Thu Ya Win LWIN Department of Pharmacy NUS

10 Ms Yang WANG China Pharmaceutical University

1645 ndash 1715 Faculty Speaker 6The thioredoxin system ndash a friend of foe in disease states

Prof Eng Hui CHEW Associate ProfessorDepartment of Pharmacy NUS

1715 ndash 1725 Awards Ceremony1725 ndash 1735 Closing Address Ms Wen Chin FOO

Chairperson AAPS-NUS Student Chapter1800 1815 Shuttle Bus Time Schedule

Area 39 MTR Station

1

Faculty speaker 1

Haishu LIN 林海树

Assistant Professor

Department Department of Pharmacy

Institution National University of Singapore Address Department of Pharmacy

National University of Singapore

18 Science Drive 4

Singapore 117543

Tel (+65) 6516 6537

Fax (+65) 6779 1554

Email phalhnusedusg

Title of presentation Naturally occurring stilbenes beyond resveratrol superior anti-inflammatory potencies and

favorable pharmacokinetics

Biography Dr Lin Haishu received his BSc from Sun Yat-Sen University in 1997 He completed his graduate

study in the Department of Pharmacy National University of Singapore After he got his PhD in

2002 he furthered his post-doctoral training in NUS Monash University (Melbourne) and

ProSkelia (Paris) His current research is focused on anti-inflammatory pharmacology

pharmaceutical analysis and pharmacokinetics Dr Lin has more than 40 publications on peer

reviewed journals and serves as a reviewer for more than 30 international journals In this talk he

shall be discussing selected research work in his laboratory

2

Faculty speaker 2

Wing Yan (Vivian) LEE 李詠恩

BSc PharmD BCPS (Added Qualification in Cardiology)

Associate Professor

Assistant Dean (Student Development) Faculty of Medicine

Department School of Pharmacy

Institution The Chinese University of Hong Kong Address 8th Floor Lo Kwee-Seong Integrated Biomedical

Sciences Building Area 39 The Chinese University of Hong Kong Shatin NT Hong Kong

Tel (+852) 3943 8012

Fax (+852) 2603 5295

Email vivianleecuhkeduhk

Title of presentation Impact of community outreach for atrial fibrillation

Biography Dr Vivian Lee is currently Associate Professor of the School of Pharmacy and the Assistant Dean

(Student Development) of the Faculty of Medicine Chinese University of Hong Kong She joined

the School of Pharmacy since 2000 Before her current appointment she had worked as a

hospital clinical pharmacist at an acute-care hospital Cedars-Sinai Medical Center in Los

Angeles Dr Lee received her bachelor of sciences degree in Biochemistry at the University of

California Los Angeles (UCLA) and her doctor of pharmacy degree in the School of Pharmacy

University of Southern California (USC) After completion of her pharmacy training she had

pursued post-doctoral training in Pharmacy Practice residency for one year at the Huntington

Memorial Hospital in Pasadena USA Her specialty is in clinical pharmacy in particular to

cardiovascular medicine Dr Lee is a registered pharmacist in both United States and Hong Kong

She is also a certified Specialist by the Board of Pharmaceutical Specialties (US) in

Pharmacotherapy and added qualification in cardiology pharmacotherapy

3

Dr Lee is the member of the Hong Kong Pharmacy and Poison Board and the assessment panel

member of the Hong Kong Social Workers Registration Board She also serves as the President

of the International Society of Pharmacoeconomics and Outcome Research (ISPOR) ndash Hong

Kong Chapter and the member of the Executive Committee ISPOR Asia Consortium Dr Lee is

also the section chairperson for the Pharmacy Education and Student Affairs of the Federation of

Asian Pharmaceutical Association

Dr Lees research interest is in clinical pharmacy outcomes research in particular to chronic

cardiology disease management utilizing the skills of clinical pharmacy pharmacoeconomics

pharmacogenomics and innovative technology to improve patient care She has 98 full paper and

170 abstracts publications She is a dedicated teacher and researcher in pharmacy practice and

has received numerous teaching awards including the University Education Award (Highest

Award on Education at the Chinese University of Hong Kong) 2014 Vice Chancellorrsquos Exemplary

Award 2010 and Master Teacher Award 2012 at the Chinese University of Hong Kong Her

research achievements are also recognized by both local and international awards

4

Faculty speaker 3

Hwee-Lin WEE 黄慧琳 Assistant Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554

Email phawhlnusedusg

Title of presentation Paying for Stratified Medicine Seeking A Delicate Balance

Biography Dr Wee Hwee Lin obtained her BSc(Pharm)(Hons) and PhD from the Department of Pharmacy National

University of Singapore (NUS) in 2001 and 2006 respectively She is currently an Assistant Professor in

the same Department and a joint Assistant Professor at the Saw Swee Hock School of Public Health NUS

She seeks to inform policy making through translational research with active engagement of the

stakeholders including clinicians patients and policy makers In 2011 Dr Wee received the International

Society for Quality of Life Research (ISOQOL) Young Investigator Award and she served as an elected

member of the Board of Directors of ISOQOL from 2013-2015 She was also an associate editor of Health

and Quality of Life Outcomes (2012 ndash 2016) a BMC journal on health-related quality of life

Dr Weersquos research encompasses health-related quality of life cost-effectiveness analyses patient

preferences and medication adherence Her current work involves understanding patient preferences for

alternative high cost cancer treatments womenrsquos preferences for gene testing for non-familial breast cancer

risk and value of high cost therapy from patientrsquos perspective She is currently a member of the International

Society for Pharmacoeconomics and Outcomes Research (ISPOR) Personalized Medicine Working Group

5

Faculty speaker 4

Wai Yip (Thomas) LEE 李偉業 Assistant Professor




Tel (+852) 3943 9795

Fax (+852) 2603 5295

Email thomasleecuhkeduhk

Title of presentation Drug Delivery for Proliferative Vitreoretinopathy Biography Prof Thomas Lee joined the School of Pharmacy at the CUHK in 2013 He graduated with

BPharm (Hons) Degree from the formerly Department of Pharmacy at the CUHK and received

his PhD in Pharmaceutical Sciences (drug Delivery) from the University of Wisconsin-Madison

in USA Before joining the School of Pharmacy Prof Lee spent a decade in two multinational

pharmaceutical companies After almost 6 years at Novartis Pharmaceuticals Corporation he

was recruited to Celgene Corporation to serve as a manager of the Formulations RampD

6

Faculty speaker 5 Pei Shi ONG 王珮曦

Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554

Email phaopsnusedusg

Title of presentation Combinatorial Therapy Incorporating Histone Deacetylase Inhibitor (HDACI) For More Effective

Cancer Treatment

Biography Dr ONG Pei Shi obtained her BSc (Pharm) (Hons) from the National University of Singapore

(NUS) After a stint as a hospital pharmacist she pursued her PhD in cancer pharmacology at

NUS Following her PhD Dr Ong received post-doctoral training in cancer pharmacogenomics at

Weill Cornell Medical College and the University of North Carolina at Chapel Hill USA During

which she was also admitted as a Board Certified Pharmacotherapy Specialist in the United

States She joined the Department of Pharmacy NUS in 2010 and is currently a Lecturer with the

Department Dr Ongrsquos research interests are focused on the areas of characterization of efficacy

and toxicity profiles of chemotherapeutics in patients identification of factors such as genetic

variants that influence drug response and exploration of the use of novel combinatorial

chemotherapeutics for cancer treatment By integrating clinical observations with bench findings

she actively seeks to translate these discoveries for advancing patient care In this talk she will

be discussing selected research work from her laboratory

7

Faculty speaker 6

Eng Hui CHEW 周颖慧

Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554

Email phacehnusedusg

Title of presentation The thioredoxin system ndash a friend of foe in disease states Biography Associate Professor CHEW Eng Hui received her BScPharmacy with Honours degree from the

National University of Singapore (NUS) Singapore Upon completing her doctorate in drug

discovery at the University of Nottingham UK she joined Professor Arne Holmgrenrsquos lab as a

postdoctoral fellow at Karolinska Institute Sweden to further her studies in redox signaling In

2008 she joined NUS as Assistant Professor at the Department of Pharmacy and was appointed

Associate Professor in 2015 AProf Chewrsquos research interests are focused on the study of cellular

redox signaling and drug discovery involving elucidation of mechanism(s) of action of novel

compounds For this her particular interest has been focused on exploring the potential of

developing naturally occurring or semi-synthetic electrophilic compounds into chemotherapeutics

cytoprotective neuroprotective andor anti-inflammatory agents through their redox modulating

activities In this talk she shall be discussing selected research work in her laboratory

8

List of Oral Presentations No Abstract Title Presenting Author

OP 1 Naturally occurring stilbenes beyond resveratrol superior anti-inflammatory

potencies and favorable pharmacokinetics H S Lin

OP 2 Translational Pharmacokinetic Model for the Predictions of DB213 Nose-to-brain

Transport Q Wang

OP 3

An in vitro herb-drug interaction approach on HPLC fingerprint comparison in liver

microsomes and its validation in rats using ShensongYangxin Capsule and

Verapamil

Z Qiao

OP 4 Towards the development of novel inhibitors for Chikungunya virus infection Q T N Tran

OP 5 Impact of community outreach for atrial fibrillation V W Y Lee

OP 6 Prediction of subject classification performance of mixture model in NONMEM K H Hui

OP 7 Effect of Microneedle Geometry and Body Curvatures on Skin Penetration for the

Development of a 3D-Printed Personalised Microneedle Eye Patch S H Lim

OP 8 Adipocytes-derived Collagen Reorganization in Microenvironment Promote Breast

Cancer Metastasis X Wei

OP 9 Paying for Stratified Medicine Seeking A Delicate Balance H L Wee

OP 10 Drug Delivery for Proliferative Vitreoretinopathy W Y Lee

OP 11 Excipient Selection in Tableting The Importance of Making a Judicious Choice T N Hiew

OP 12

Oligonucleotides-conjugated Poly(ethylene oxide)-block-poly(amp949-

caprolactone) (PEG-b-PCL) Nanoparticles as Drug Carriers Targeting Scavenger

Receptor Class A

H Y Li

OP 13 Combinatorial Therapy Incorporating Histone Deacetylase Inhibitor (HDACI) For

More Effective Cancer Treatment P S Ong

OP 14 Increased FAM3C Expression Promotes Proliferation and Metastasis of Lung

Cancer Cells through Activation of RalGDSRalA Signaling Pathway T W Lwin

OP 15

Cooperative down-regulation of ribosomal protein L10 and NF-κB signaling

pathway is responsible for the anti-proliferative effects by DMAPT in pancreatic

cancer cells

Y Wang

OP 16 The thioredoxin system ndash a friend of foe in disease states E H Chew

9

List of Poster Presentations

No Abstract Title Presenting Author

PP 1 A Novel Microtubule Inhibitor that Overcomes Multi-Drug Resistance Exhibits Anti-Tumor Activity based on Intracellular Pharmacokinetic

X Zang

PP 2 The Establishment of a stable Nav15-expressed Human Embryonic Kidney 293 cell line

XY Zhang

PP 3 Argirein drug for diabetic microvascular disease on Apoptosis via Nox4-Dependent ROS in Rat aortic endothelium cells

J Su

PP 4 Mutagenesis of precursor peptide for the generation of nosiheptide analogues S Wang

PP 5 The pharmacodynamic material basis and pharmacological mechanisms of Goutengsan in treatment of type 2 diabetes induced cognitive and emotional disorders

H Zhao

PP 6 PK-PD based mechanism of synergistic effects of Shenmai injection to chemotherapeutic drugs

WY Liu

PP 7 NAD biosynthesis combined with glucuronidation metabolism contributes to the detoxication of berberrubine in kidney

Y Na

PP 8 Curcumin attenuates liver fibrosis via suppression of succinate-associated HIF-1α induction in HFD-fed mice

L She

PP 9 Liver injury of Dictamnine in mice and cytotoxicity of Dictamnine in wild-type and CYP3A4 over-expressed cells revealed the role of CYP3A4 in induced-toxicity

Z Li

PP 10 Mechanisms of dronedarone-induced cardiac adverse exacerbation Role of mitochondrial toxicity

A Karkhanis

PP 11 Perturbation of arachidonic acid metabolism by antiarrhythmic drugs Implications in cardiac safety

A Karkhanis

PP 12 Quantification of desoxyrhapontigenin (4-methoxyresveratrol) in rat plasma by LCMSndashMS Application to pre-clinical pharmacokinetic study

Y Dai

PP 13 Inhibition of 5-lipoxygenase prevents lipopolysaccharide induced depressive-like behaviors and neuroinflammation in mice

D Li

PP 14 Protective effect of Icariin on renal function in rats with type 2 diabetic nephropathy induced by HFD combined with low dose STZ via insulin signaling pathway Q Wang

PP 15 Research on Influence Factors of Hospital Pharmacistsrsquo Behavior on Clinical Pharmaceutical Care

L Wu

PP 16 Redox-responsive supramolecular hydrogel based on 10-hydroxy camptothecin-peptide covalent conjugates with high loading capacity for drug delivery

C Wu

PP 17 Mesoporous silica nanoparticles incorporated hybrid monolithic stationary phase immobilized with pepsin for enantioseparation by capillary electrochromatography

S Xu

10

PP 18 A dual-targeting drug co-delivery system for tumor chemo- and gene combined therapy

F Zhang

PP 19 The unit-dose plate for compounding orodispersible films in the pharmacy ndash a human factors evaluation

W C Foo

PP 20 Nanoparticles of a novel polyethoxy-based polymer synthesized by microwave assisted polymerization CXY Wong

PP 21 Dissolvable Microneedles for Transdermal Immunotherapy H Kathuria

PP 22 The influence of lipid modifiers on the rheological properties of molten paraffin wax and the degree of drug coating in resultant spray-congealed microparticles

H Ouyang

PP 23 Orally-Dissolving Films for the Delivery of Ropinirole B Zhang

Map to the canteen

11

OP 1 Naturally occurring stilbenes beyond resveratrol superior anti-inflammatory potencies and

favorable pharmacokinetics HS Lin

Department of Pharmacy National University of Singapore Singapore

OBJECTIVE Resveratrol (RES) a dietary polyphenol is well known for its numerous health-promoting activities Due to

its relatively weak potency and inferior pharmacokinetics its clinical application has not been established

in any medical condition Our research program aims to identify RES derivatives with superior anti-

inflammatory potency and improved pharmacokinetic profiles for further pharmaceutical development

METHODS The anti-inflammatory activities of RES and its naturally occurring derivatives were assessed in various in

vitro models The pharmacokinetic profiles of the RES derivatives with promising in vitro anti-inflammatory

effects were examined in Sprague-Dawley rats

RESULTS In rheumatoid arthritis (RA) related models all tested stilbenes exhibited concentration-dependent anti-

rheumatic activities Of note the anti-inflammatory potencies of pterostilbene (PTS) and resveratrol

trimethyl ether (RTE) were much stronger than RES Similarly in chronic obstructive pulmonary disease

(COPD) related models isorhapontigenin (IRG) displayed a disease-modifying effects superior to RES

The pharmacokinetic profiles of RTE PTS and IRG were subsequently examined All these naturally

occurring RES derivatives displayed pharmacokinetic profiles more favorable than RES

CONCLUSION In conclusion RTE PTS and IRG have been identified as promising candidates for further drug

development

12

OP 2 Translational Pharmacokinetic Model for the Predictions of DB213 Nose-to-brain Transport

Qianwen Wanga Yufeng Zhanga Chun-Ho Wongb HY Edwin Chanbc Zhong Zuoa

aSchool of Pharmacy bSchool of Life Sciences and cGerald Choa Neuroscience Centre

The Chinese University of Hong Kong Shatin NT Hong Kong

OBJECTIVE A translational pharmacokinetic (PK) model could efficiently reconcile data from different species The

current study aims to develop a pharmacokinetic model feasible to be translated between rats and mice to

demonstrate and quantify direct nose-to-brain DB213 (an HIV-1 replication inhibitor targeting HIV-

associated neurocognitive disorders) transport via intranasal (IN) administration

METHODS SD rats were administered with DB213 water solution via IN or intravenous (IV) at 1 10 and 50 mgkg

while C57BL6 mice received DB213 water solution via IN at 25 mgkg Plasma and brain samples were

collected up to 8 h post-dosing

Structural model building was performed using data sets of SD rats by NONMEM Based on PK model

developed in rats PK parameters of mice were obtained by allometric scaling followed by visual predictive

check If mice PK parameters cannot be translated from rats directly they will be obtained by fitting the

experimental data in the developed model

RESULTS In rats AUC0rarr480 minplasma were 11768plusmn902 and 1024plusmn132 μgminmL while AUC 0rarr480 minbrain were 18plusmn6 and

43plusmn9μgming for IV and IN respectively In mice AUC0rarr480 minplasma and AUC 0rarr480 minbrain were 377plusmn43

μgminmL and 121plusmn19 μgming

The model with two absorption compartments (nose-to-systemic circulation and nose-to-brain) could best

describe DB213 pharmacokinetics in rats with the lowest OFV values (-58063 points) 7236 of the total

absorbed DB213 was transported directly into the brain in rats

DB213 distribution and elimination in mice was well predicted using PK parameters obtained by allometric

scaling of rat PK parameters The absorption rate constants estimated from mice experimental data were

found to be 56 fold higher than that was allometrically scaled which indicates faster nose-to-systemic

circulation and nose-to-brain absorption in mice than that in rats 5128 of the total absorbed DB213 was

transported directly into the brain in mice

CONCLUSION A translational PK model was developed to demonstrate direct nose-to-brain DB213 transport in both rats

and mice via intranasal administration (Financial support Lui Che Woo Institute of Innovative Medicine

BRAIN Initiative CUHK Project Number 8303404)

13

OP 3 An in vitro herb-drug interaction approach on HPLC fingerprint comparison in liver microsomes

and its validation in rats using ShensongYangxin Capsule and Verapamil Zhou Qiaoa 1 Mei Zhenga 1 FengFengb JingweiXuec XinWanga FuleiLiubc Wenyuan Liu a

aKey Laboratory on Protein Chemistry and Structural Biology China Pharmaceutical University Nanjing 210009 China bKey Laboratory of Biomedical Functional Materials China Pharmaceutical University Nanjing 211198 China

cTaian City Central Hospital Shandong 271000 China

OBJECTIVE Traditional Chinese Medicine (TCM) is composed of multi-components and it is difficult to evaluate the

interaction of co-administered synthetic drug on TCM in vitro The purpose of this study was to establish an

approach on chromatographic fingerprint comparison to investigate the possible herb-drug interactions

between ShensongYangxin Capsule (SSY) and verapamil(VER) in liver microsomes

METHODS SSY and VER were incubated simultaneously with rat liver microsomes Analytes were extracted by

protein precipitation and chromatographic fingerprints of them were acquired by HPLC To evaluate the

effect of VER on the metabolism of SSY the similarity of the fingerprinting chromatograms before and after

co-incubation was calculated by software Furthermore pharmacokinetic experiments in rat were also

conducted in the study to confirm the results obtained with liver microsomes Two main components of SSY

have been simultaneously quantified by a developed LC-MSMS method in rat plasma Their

pharmacokinetic parameters were compared in rats before and after co-administered in rats to evaluate the

interaction between SSY and VER

RESULTS HPLC fingerprinting chromatograms of SSY in active and inactivated RLMs The value of similarity between

them was 085 calculated by the similarity evaluation system for chromatographic fingerprint of TCM which

indicated that SSY could be metabolized by RLMs in vitro When VER was added to the incubation medium

the similarity was 097 The parameter Cmax t12 AUC AUMC for schisandrin A and deoxyschizandrin in

the combined administration group was much larger than those in single administration group

CONCLUSION An approach based on HPLC fingerprints has been established and validated to evaluate the herb-drug

interactions between SSY and VER in RLMs for the first time Our results indicate that concomitant use of

SSY and VER inhibits the metabolism of SSY which may result in decreased efficacy The pharmacokinetic

study of SSY and VER further confirmed the results

14

OP 4

Towards the development of novel inhibitors for Chikungunya virus infection Quy Thi Ngoc Tran1 Kuan-Chieh Ching12 Lisa F P Ng3 and Christina L L Chai1

1Department of Pharmacy Faculty of Science National University of Singapore Block S4A Level 3

18 Science Drive 4 Singapore 117543 2NUS Graduate School for Integrative Sciences and Engineering Centre for Life Sciences 05-01 28

Medical Drive Singapore 117456 3Singapore Immunology Network ASTAR 8A Biomedical Grove Immunos Building Level 4

Singapore 13864

OBJECTIVE Chikungunya virus (CHIKV) infection is currently one of the most challenging human Arboviral infections

with frequent outbreaks in tropical countries of Africa and Southeast Asia The recent re-emergence and

travel-related spread of CHIKV has drawn global attention and the disease threatens to expand in the

foreseeable future Driven by the medical importance of this virus as well as the lack of approved effective

antivirals research into the field of CHIKV antivirals has been intensified in recent years This study aims

to discover new CHIKV inhibitors with enhanced potency and improved in vitro metabolic stability

METHODS We conducted a Scaffold Hopping program around the thieno[32-b]pyrrole scaffold Structure-activity and

structure-metabolism relationship studies were carried out for a novel series of N-propyl-isonipecotamide

based CHIKV inhibitors developed through this approach

RESULTS Key to this development was the identification of novel pyrrolo[23-d]thiazo-based compounds that inhibit

CHIKV replication in low micromolar range These compounds exhibited potent inhibitory activity against in

vitro CHIKV infection with EC50 values as low as 1 μM and improved in vitro metabolic stability in a

preliminary pharmacokinetic study using human liver microsomes (HLMs)

CONCLUSION Results from this study represent a substantial advancement in the early preclinical development of a new

class of novel antiviral drugs against Chikungunya infection

15

OP 5

Impact of community outreach for atrial fibrillation V W Y Lee

School of Pharmacy Faculty of Medicine The Chinese University of Hong Kong

OBJECTIVE To estimate the prevalence and knowledge of atrial fibrillation(AF) in Hong Kong elderly population and

evaluate the effects of a pharmacy outreach intervention on AF knowledge and therapy adherence in those

with AF

METHODS We recruited subjects aged 65 or above from 26 elderly centers in Hong Kong between 2013-15 Screening

of AF was done using handheld ECG device to estimate the prevalence of AF The demographics of AF

and non-AF participants were compared to identify risk factors The baseline AF knowledge risk factors

control chronic medications and adherence of AF subjects were recorded AF knowledge was assessed

using a novel tool Education sessions and referral letters for anticoagulant initiation were provided in first

follow up The impacts of interventions were evaluated in follow up including reassessment of AF knowledge

blood pressure and blood glucose readings anticoagulant use and medication adherence

RESULTS 147 out of 2767 subjects (53) were detected with AF and almost all (51) were newly diagnosed AF

The mean age of AF patients was 803 plusmn 71 years old as compared to non-AF patients was 772 plusmn 73

years old Those with AF were more likely to be aged gt85 (OR=254) male (OR=165) and have

hypertension (OR=155) Mean score for correct AF knowledge items was 79 Those with AF had a

slightly higher score (128 vs 78 for non-AF population p= 0143) AF knowledge improved from 128

to 376 after intervention (plt0001) in those with AF The mean adherence score improved after the

intervention demonstrating significant medication adherence improvement (p =0008)

CONCLUSION The prevalence of AF in this elderly Chinese population was similar to that found in other populations in

other countries The pharmacy outreach service had significant impacts with improved AF knowledge and

medication adherence post-intervention in those with AF

16

OP 6 Prediction of subject classification performance of mixture model in NONMEM

K H Hui T N Lam


OBJECTIVE An important limitation of Fitting a mixture model in NONMEM is inaccurate subject classification where

low classification accuracy (CA) may also lead to biases of estimators However CA cannot be obtained

directly for it is presumption-based Besides a standard way to predict CA had not been available This

study aimed to develop a prediction equation for classification accuracy when fitting a mixture model

presuming a bimodal clearance distribution to pharmacokinetic data under the setting of a one-compartment

model with first-order absorption using NONMEM

METHODS 2048 pharmacokinetic datasets with various study design parameters (sample size and number of samples

per subjects) pharmacokinetic parameters (typical value of clearance volume of distribution and

absorption rate constant) and statistical parameters (coefficient of variance of inter- and intra-individual

variabilities) were simulated Since these datasets were simulated true classifications were known

Classifications by NONMEM were then obtained by fitting a mixture model to datasets after which CA was

calculated for each dataset The relationships between CA and various factors (including the above

parameters and other outputs such as the change in object function value when the mixture model was

removed dOFV) were then investigated and quantified with a prediction equation developed with NONMEM

RESULTS The most predictive factor identified was dOFV (accounting for 941 of total variability explained) Model

diagnostics including predictive plots studentized residual plots internal validation (bootstrapping) and

external validation (using separately simulated datasets where the studentized residual in 933 of

datasets fell into the 95 prediction interval) demonstrated the stability and robustness of the final

prediction model

CONCLUSION Modelers should take into account the risks associated with low CA and biased estimators when using the

mixture model in NONMEM It is recommended that data should always be refitted without the mixture to

obtain the dOFV for mixture model assessment

17

OP 7 Effect of Microneedle Geometry and Body Curvatures on Skin Penetration for the Development of

a 3D-Printed Personalised Microneedle Eye Patch S H Lim1 W J Tiew1 L Kang1

Department of Pharmacy Faculty of Science National University of Singapore

OBJECTIVE To develop a 3D printed personalized microneedle eye patch for transdermal drug delivery in the potential

treatment of peri-orbital wrinkles

METHODS 3 curvatures A) gentle B) intermediate and C) sharp were chosen to represent different body curvatures

Curved patches with various microneedle geometries were designed and fabricated via

photopolymerisation-based 3D-printing for each curvature These patches were assessed for their

respective mechanical strength and skin penetration efficiency to determine the optimal microneedle

geometry for each curvature A computer aided design (CAD) head model was obtained via the use of a

handheld 3D scanner and utilized for the design of a personalized microneedle eye patch with the optimal

microneedle geometry The fabricated microneedle eye patch was characterized and comparison to a

commercial flexible microneedle eye patch was made for its transdermal drug delivery potential using

confocal microscopy

RESULTS In general regardless of body curvatures a longer sharper and more widely spaced microneedle achieved

a better skin penetration efficiency Furthermore for each microneedle geometry patches of intermediate

curvatures consistently achieve the best skin penetration efficiency compared to the gentle or sharp

curvatures In all the optimized geometry was determined to be that of microneedle length 800μm tip

diameter 100μm interspacing 800μm and base diameter 400μm The eventual 3D-printed personalized

microneedle eye patch was designed based on the optimized geometry and the facial information of the

CAD model Confocal imaging of the skin sample after in vitro skin permeation using a fluorescence dye

demonstrated enhanced transdermal delivery compared to a commercial flexible microneedle eye patch

CONCLUSION Microneedle geometry for curved microneedle patches is critical for efficient skin penetration Using an

optimized geometry and actual patient scans we also demonstrated the use of 3D scanning and 3D printing

technology to fabricate a personalized microneedle eye patch for transdermal drug delivery for peri-orbital

wrinkles

18

OP 8 Adipocytes-derived Collagen Reorganization in Microenvironment Promote Breast Cancer

Metastasis Xiaohui Wei Shengtao Yuan Li Sun

Jiangsu Key Laboratory of Drug Screening China Pharmaceutical University Nanjing Jiangsu China

OBJECTIVE Breast cancer cells recruit surrounding stromal cells such as cancer-associated fibroblasts (CAFs) to

reorganize collagen and promote tumor metastasis yet their precise origins and relative functional

contributions to malignant progression remain uncertain Adipocytes are the most abundant stromal

partners in breast tissue whether cancer-associated adipocytes (CAAs) involve in the collagen

reorganization and thus promoting metastasis of breast cancer is still unknown In this study we seek to

understand whether adipocytes involve the extracellular matrix reorganization in breast cancer and its

underline mechanism

METHODS A Transwell co-culture model in vitro and orthotopic mouse model of human breast tumor formation in vivo

were used to study the crosstalk between adipocytes and breast cancer cells and then using a combination

of proteomics database analysis and 180 clinical breast cancer sample analysis to study the mechanism

mediated adipocytes-derived collagen reorganization

RESULTS We show that adipocytes cocultivated with breast cancer cells (MDA-MB-231 SK-BR-3 MDA-MB-468 and

BT-474) exhibited enhanced secretion of collagen I and accompanied by increasing collagen lysyl

hydroxylation 2 (LH2) and lysyl oxidase (LOX) expression while tumor cells cocultivated with CAAs in two-

dimensional or spheroid culture display increased invasive capabilities The correlation of LH2 with

prognosis was analyzed by the KaplanndashMeier plotter the data suggested that LH2 was associated with

poor prognosis of breast cancer patients Simultaneously 180 clinical samples analysis showed LH2 was

high expression in CAAs and with poor prognosis of patients Then using cytokine antibody microarrays to

explore the mechanism mediated adipocytes-derived collagen reorganization and it showed mostly

cytokine secreted in MDA-MB-231 cells were IL-6IL-8PAI-1TIMP1TIMP2 may involve the generation of

the adipocyte-derived collagen reorganization Together our results offer new opportunities for stroma-

targeted therapies in breast cancer

CONCLUSION Lysyl hydroxylase 2 which drives collagen reorganization was expressed in CAAs and breast tumor-

derived cytokine IL-6IL-8PAI-1TIMP1TIMP2 may involve in the activation of Lysyl hydroxylase

19

OP 9 Paying for Stratified Medicine Seeking A Delicate Balance

H L Wee

Department of Pharmacy National University of Singapore Saw Swee Hock School of Public Health

The use of genetic information to improve treatment efficacy and reduce treatment side effects is

increasingly prevalent and belongs to a field that is being referred to as personalised stratified

precision medicine The high cost of targetted therapy such as trastuzumab for the treatment of

HER2 positive metastatic breast cancer has hit the headlines of major newspaper and social

media While stratified medicine such as targetted therapy holds promise for reducing total

healthcare cost by avoiding ineffective treatment among individuals who do not carry the specific

gene mutation the huge price tag that they carry is likely to offset the potential cost savings Drug

manufacturers cite the need to invest in high risk research and development and the smaller pool

of potential customers to justify such high price tag How should payers determine what to pay for

without unduely limiting access to treatment A multicriteria decision framework for paying for

preemptive pharmacogenotyping will be discussed

20

OP 10 Drug Delivery for Proliferative Vitreoretinopathy

W Y Lee


OBJECTIVE We aimed to apply nanoparticles to deliver therapeutic agents for the treatment or prevention of proliferative

vitreoretinopathy

METHODS Drug-loaded PEG-b-PCL micelles were fabricated and physically characterized These nanoparticles were

further assessed for ocular safety and in vitro efficacy Finally their in vivo efficacy for the management of

proliferative vitreoretinopathy was verified in a mouse model

RESULTS The drug-loaded micelles were nano-sized with a narrow particle size distribution They enhanced the

solubility of the model drug by gt500x 1H NMR and zeta potential measurements confirmed that the drug

was embedded into the cores of the micelles These nanoparticles were physically stable in the vitreous

and exhibited sustained drug release They were shown to be safe both in vitro and in vivo Most importantly

the drug-loaded PEG-b-PCL micelles significantly enhanced the efficacy for the management of

proliferative vitreoretinopathy in a mouse model of proliferative vitreoretinopathy

CONCLUSION The drug-loaded PEG-b-PCL micelles appear to be safe for ophthalmic use and they also exhibit

remarkable efficacy for the treatment or prevention of proliferative vitreoretinopathy for which there is not

approved drug therapy Taken together the drug-loaded PEG-b-PCL micelles hold considerable promise

in the management of proliferative vitreoretinopathy Further development of this novel therapy for clinical

use should be considered

21

OP 11 Excipient Selection in Tableting The Importance of Making a Judicious Choice

TN Hiew PWS Heng

Department of Pharmacy National University of Singapore

OBJECTIVE The purpose of this study was to investigate the effect of excipients with different moisture sorption profiles

on the stability of hydrolyzable drugs when exposed to environment of various relative humidity conditions

In addition the effect of storage humidity on the physical stability of the dosage form such as its tensile

strength thickness and weight was also investigated

METHODS Acetylsalicylic acid (ASA) was chosen as the model for hydrolyzable APIs ASA tablets were prepared with

8 excipients as diluents Well mixed samples of 300 mg mixed tablet feed were individually weighed and

compressed using 10 mm flat face punches to 1873 MPa These tablets were stored at 53 and 75

relative humidity (RH) for 3 months at 25 degC and evaluated at selected time points The resultant tablets

were evaluated for their physical mechanical and dimensional attributes as well as chemical stability

RESULTS Among the excipients used the change in moisture content of starch MCC and XPVP were found to exhibit

environmental dependence while mannitol sorbitol DCP sucrose and lactose generally do not

Regardless of the excipient used storage humidity of 75 RH was found to be relatively more detrimental

to the tablets In particular tablets containing XPVP showed the most drastic physical changes significantly

increased tablet weight and thickness with weakened tablet matrix In addition these tablets also exhibited

the greatest extent of ASA degradation regardless of storage humidity

CONCLUSION This study has shown that the judicious selection of excipients is critical when APIs susceptible to hydrolysis

are concerned Excipients sensitive to changes in ambient humidity changes were the most detrimental to

these APIs Thus if the use of these excipients is needed for any particular reasons careful packaging may

be required to ensure that the dosage form is well protected from environmental humidity

22

OP 12 Oligonucleotides-conjugated Poly(ethylene oxide)-block-poly(amp949-caprolactone) (PEG-b-PCL)

Nanoparticles as Drug Carriers Targeting Scavenger Receptor Class A H Y Li Z Chen L W Ho Q Lee P S Chan C H Choi and W Y Lee

School of Pharmacy The Chinese University of Hong Kong

OBJECTIVE Oligonucleotides have high affinity towards scavenger receptors Class A (SR-A) which are abundant in

various epithelial and endothelial cells Herein we reported the use of paclitaxel (PTX)-loaded

oligonucleotides-conjugated nanoparticles for targeted delivery to SR-A expressing cells for inhibition of

angiogenesis

METHODS Oligonucleotides-conjugated PEG-b-PCL was prepared via Michael addition reaction of oligonucleotides

onto acrylate functionalised PEG-b-PCL The nanoparticles were prepared by an anti-solvent method The

nanoparticles were characterised for particle size and polydispersity by DLS and the drug loadings and

encapsulation efficiency were determined by UPLC-UV C166 an endothelial cell line with positive

expression of SR-A was used in this study A cellular uptake study was carried out using coumarin-6 loaded

nanoparticles The efficacy against angiogenesis of each formulation was determined using in vitro

proliferation and migration assays

RESULTS Oligonucleotides-conjugated PEG-b-PCL nanoparticles (ODN-Np) have a mean particle size of 581plusmn05

nm with a polydispersity index of 0156plusmn0022 and a zeta potential of -309plusmn30 mV ODN-Np improved the

solubility of PTX by 200x ODN-Np has shown 394x improvement in cellular uptake compared to PEG-Np

Further investigation on the uptake mechanisms using endocytosis inhibitors indicated that the uptake

improvement is mainly attributable to CAV-1 and SR-A mediated endocytosis In the proliferation assay

PTX-loaded ODN-Np showed improved efficacy compared to PTX-loaded PEG-Np (At 100 m gmL PTX

ODN-Np = 320plusmn34 proliferation vs PEG-Np = 485plusmn68 proliferation) PTX-ODN-Np showed significant

improvement in inhibiting cellular migration compared with PTX-PEG-Np and the no treatment control (125

m gmL PTX After 36 hours ODN-Np = 449plusmn15 inhibition PEG-Np = 261plusmn44 inhibition and control =

154plusmn55 inhibition respectively)

CONCLUSION ODN-Np improved the solubility of PTX by 200x ODN-Np showed an enhanced cellular uptake and better

efficacy in inhibiting cell proliferation and migration in vitro The improvement is mainly attributable to the

presence of ODN as a targeting ligand for SR-A receptors

23

OP 13 Combinatorial Therapy Incorporating Histone Deacetylase Inhibitor (HDACI) For More Effective

Cancer Treatment PS Ong


Despite tremendous progress made in the control of cancer progression in the last few decades cancer

continues to be a leading cause of death worldwide This suggests an urgent need to better understand the

biology of this heterogeneous group of diseases and the necessity to discover more novel effective

therapeutic agents for cancer treatment In recent years it has been increasingly recognised that aberrant

epigenetic regulation of tumour-associated genes via histone hypoacetylation is an important process

contributing to tumour development and its continual growth This has in turn led to the development of a

new mechanistic class of anticancer agents termed the histone deacetylase inhibitors (HDACIs) for tumour

eradication HDACIs have been known to work via chromatin relaxation to increase the accessibility of

transcription factors to the DNA structure thereby permitting the re-expression of important genes that were

silenced during tumour formation By doing so HDACIs act to eradicate cancer via activation of multiple

antitumour pathways hence bringing about cancer cell cycle arrest and cell death Notwithstanding these

promising anticancer mechanisms effects of several HDACIs when used as a single agent in clinic have

been modest in solid tumours in part due to their inferior pharmacokinetic profiles suggesting the need to

explore new approaches to extend their therapeutic utility One of the aims of our laboratory is to identify

rational combinatorial therapy incorporating HDACI that allows for superior cancer cell kill at a suitable

HDACI concentration that is achievable clinically Thus far we found that HDACIs such as BelinostatOgrave at

clinically relevant concentrations effectively augmented the antitumour cell kill of conventional and other

newer chemotherapeutics via triggering of apoptotic pathways as well as downregulation of antiapoptotic

proteins These novel regimens thus warrant further investigation for future development

24

OP 14 Increased FAM3C Expression Promotes Proliferation and Metastasis of Lung Cancer Cells through

Activation of RalGDSRalA Signaling Pathway Thuya Win Lwin 1 2 Ross A Soo 2 3 Lingzhi Wang2 4 Boon Cher Goh2 3 4 Paul Chi-Lui Ho1

1Department of Pharmacy National University of Singapore Singapore 2Cancer Science Institute of Singapore National University

of Singapore Singapore 3Department of Hematology-Oncology National University Health System 4Department of Pharmacology

National University of Singapore Singapore

OBJECTIVE High mortality rate of lung cancer is due to increased metastasis initiated by EMT FAM3C (ILEI)

Interleukin-like EMT inducer promotes EMT in cancer cells but its underlying mechanism remains unknown

This study aimed to investigate the underling mechanism of FAM3C in inducing proliferation and metastasis

of lung cancer cells

METHODS In this study shRNA transfection western blot immunohistochemistry staining phosphokinase array

wound healing assay matrigel invasion assay MTS assay colony forming cell assay Co-IP and Duolink

ligation assay were used for investigation of oncogenic properties of FAM3C in human lung cancer cell

lines Highly expressed FAM3C SKMES-1 and low expressed FAM3C H838 cell lines were used for

functional studies

RESULTS The IHC staining indicated that FAM3C expression in lung cancer specimens was greatly increased

compared to those in tumour adjacent and normal lung tissues In functional studies shRNA-silenced

FAM3C in SKMES-1 cells resulted in significant reduction of cancer cell invasion migration In addition the

FAM3C knockdown cells showed reduced cell proliferation colony formation and enhanced

chemosensitivity to cisplatin Contrastingly overexpressed FAM3C in H838 cells induced EMT cell

proliferation invasion migration and metastasis Through bioinformatics analysis the overexpression of

FAM3C was strongly associated with the upregulation of phosphorylated RalA (Ser194) which is critical for

RAS-induced tumorigenesis and metastasis of cancer cells RalA GTPase a Ras downstream signaling

molecule activates RalA binding protein 1 which subsequently activates the phosphorylation of Src and

STAT3(Tyr705) in the downstream signaling pathway Its activation was confirmed by phosphokinase

protein arrays Duolink PLA and Co-IP assay further supported the protein-protein interaction between

FAM3C and RalA Together FAM3C expression is highly correlated with the phosphorylation level of RalA

in lung cancer cells

CONCLUSION These findings demonstrate that FAM3C acts an EMT enhancer to induce lung cancer cell metastasis and

tumorigenesis via activation of RalGDSRalA and its downstream Src-STAT3 signaling pathway

25

OP 15 Cooperative down-regulation of ribosomal protein L10 and NF-κB signaling pathway is

responsible for the anti-proliferative effects by DMAPT in pancreatic cancer cells Chen Shi1 Yang Wang1 Yuna Guo1 Yijun Chen1 Nan Liu1

1State Key Laboratory of Natural Medicines and Laboratory of Chemical Biology China Pharmaceutical University Nanjing PR

China

OBJECTIVE Dimethylaminoparthenolide (DMAPT) a water-soluble analogue of natural product parthenolide possesses

anti-inflammatory and anti-tumor activities We investigated the anti-proliferative effects and the anti-tumor

mechanism of DMAPT in pancreatic cancer cell lines

METHODS The sensitivity of DMAPT against pancreatic cancer cells was evaluated by MTT and flow cytometry Serial

affinity chromatograph was implemented to probe potential targets for DMAPT The target protein was

identified by tryptic digestion and MALDI-TOF-MS analyses Immunoprecipitation western blotting and

qPCR were used to examine the interaction of proteins and the expression level of mRNA and protein After

purifying the target protein the interaction between DMAPT and protein was verified by Microscale

Thermophoresis analyses in vitro

RESULTS Ribosomal protein L10 (RPL10) is a specific binding protein of DMAPT in PANC-1 cells DMAPT could

decrease the expression of RPL10 accompanying its anti-proliferative effects Mechanistically in both

PANC-1 cells and MiaPaca-2 cells reduced expression of RPL10 triggered by DMAPT binding decreased

the expression of either p65 or IKKγ through the direct binding between RPL10 and p65 or IKKγ

CONCLUSION We have employed chemical proteomics approach to reveal the specific and direct interaction between

DMAPT and RPL10 after confirming the anti-proliferative effects of DMAPT in pancreatic cancer cell lines

RPL10 has been found to directly down-regulate the expression of p65 and IKKγ The present study

strongly implies that RPL10 is a novel target with therapeutic potential for the treatment of pancreatic cancer

26

OP 16 The thioredoxin system ndash a friend of foe in disease states

EH Chew


The thiol redox systems play a key role in maintaining intracellular redox homeostasis of which the

thioredoxin and glutathione systems are the two major systems The thioredoxin (Trx) system comprises

Trx thioredoxin reductase (TrxR) and NADPH TrxR catalyzes the NADPH-dependent reduction of the

active site disulfide of oxidized Trx To protect cellular proteins from oxidative damage Trxs use a

conserved redox active dithioldisulfide motif (-Cys-xx-Cys-) to participate in reversible thiol-disulfide

exchange reactions Emerging findings have indicated that the dysregulated levels of the components of

the Trx system has led to different disease states In our laboratory we have taken interest to investigate

the Trx systemrsquos involvement in regulating apoptosis We have found that thioredoxin-1 (Trx-1) is capable

of regulating DNA damage mediated by apoptosis inducing factor (AIF) The identification of the interaction

between Trx-1 and AIF has provided opportunities to design and develop strategies that either promote or

prevent this protein-protein interaction for treatment of different disease states While Trx is required for

regulation of cell proliferation and apoptosis on the other hand in cancer the biological effects of the Trx

system contribute to tumor growth and progression In the light of the high prevalence of cancer and

refractoriness of malignant tumors to clinical agents novel molecular targets need to be identified and new

chemotherapeutics be discovered Accumulating evidence has indicated that the selenocysteine-

dependent TrxR enzyme is a valid molecular target for anticancer drug development Numerous natural

products and synthetic compounds including several clinically used chemotherapeutics have been

recognized to target TrxR A number of structurally diversified compounds derived from natural sources

have also been found to possess potent inhibitory effect against TrxR In our laboratory we had evaluated

the possible inhibitory effect of a panel of structurally diversified naturally-occurring compounds and their

derivatives on TrxR activity Sharing in common electrophilic centers these compounds had been found to

possess TrxR inhibitory activity correlating to their antiproliferative activity On this basis these compounds

can be further developed for applications in cancer chemotherapies that may lead to more desirable clinical

outcomes

27

PP 1 A Novel Microtubule Inhibitor that Overcomes Multi-Drug Resistance Exhibits Anti-Tumor Activity

based on Intracellular Pharmacokinetic Xiaojie Zang1a Fang Zhou1a Qingyun Cai1 Jingwei Zhang1 Qianying Chen1 Xiao Zheng1 Haiping Hao1and Guangji Wang 1

OBJECTIVE As the efficacious chemotherapeutic drug PTX exerted decreased cytotoxicity due to the efflux of Paclitaxel

(PTX) executed by P-gp which substantiated the need to develop new microtubule inhibitors As a

promising microtubule inhibitor candidate Whether DPT exerted multi-drug resistance is yet to be

investigated

METHODS MTT assays and flow cytometer analysis were conducted to compare the efficacy of DPT and PTX on MCF-

7 and MCF-7Adr cells For further evaluation the MCF-7 and MCF-7Adr xenograft models were

established From the perspective of intracellular pharmacokinetics cellular accumulation of DPT and PTX

was quantitatively monitored by a novel LCMSMS method Furthermore MDCK-MDR1 cell model was

used to explore the underlying mechanism

RESULTS Distinguished from PTX DPT exerted similar cytotoxicity on both drug-sensitive and drug-resistant cell lines

Surprisingly the inhibition of DPT on MCF-7 and MCF-7Adr xenograft models was also confirmed Support

for this notion further evidence came from our findings that the subcellular distribution of DPT and PTX

exhibited distinctive difference on the accumulation in different cellular organs especially in cytosol Further

in-depth profiling was provided to demonstrate DPT was not the substrate of P-gp

CONCLUSION We provided several lines of evidence to firstly illuminate that DPT exerted proliferation inhibition effect on

MCF-7 and resistant MCF-7Adr cells in vitro and in vivo in comparison with PTX Moreover we initially

confirmed DPT was not a substrate of P-gp efflux pump and could overcome P-gp-mediated multidrug

resistance Thus as a new tubulin polymerization inhibitor DPT can be exploited as a promising agent for

the treatment of multidrug ndashresistant tumors

28


X Y Zhang1 F Zhao2

OBJECTIVE To establish the stably expressing Nav15 channel in human embryonic kidney cell (HEK 293) cell line as

a Cardiac Toxicity Screening Model for drugs

METHODS We used Lipofectaminereg 2000 for transfecting pcDNA31 Nav15 into the HEK 293 cell line and screened

the stable transfected monoclonal cell lines with Geneticin Finally we used FLIPR membrane potential

blue dye and patch clamp to detect Nav15 function

RESULTS In this study we established the stably expressing Nav15 channel in HEK 293 cell line we used the

extensive VGSC agonist Veratridine to stimulate the membrane depolarization which was detected by

membrane potential FMPblue dye recorded in FLIPR and the VGSC antagonist Tetradotoxin can

concentration-dependent inhibit the membrane depolarization induced by Veratridine which indicated the

stably expressing of Nav15 channel Futher more in patch clamp recording 10μM Tetradotoxin can not

completely inhibit the sodium current which indicated the sodium channel was TTX-resistant sodium

channel

CONCLUSION We have established Nav15 HEK293 cell line successfully which offers a stable and reliable method to

idenyify Nav15 inhibitors and develop them into drugs

29

PP 3 Argirein drug for diabetic microvascular disease on Apoptosis via Nox4-Dependent ROS in Rat

aortic endothelium cells Su Jie

China Pharmaceutical University

OBJECTIVE Our previous studies have indicated that argirein (AR) effectively normalized vessel function in diabetes

mellitus However the molecular mechanisms mediating the vascular protective role of AR remain unknown

METHODS It was shown that high glucose significantly increased the protein expression of Bax and decreased Bcl-2

protein in Rat aortic endothelium cells(RAEC) which was reversed by AR The percentage of RAECs that

showed Annexin V-FITC binding was markedly suppressed by AR which confirmed the inhibitory role of

AR on apoptosis Given the apoptosis system to oxidative stress we determined the role of redox signaling

in the regulation of AR on apoptosis

RESULTS It was demonstrated that the production of superoxide (O2-) was substantially attenuated by AR treatment

in RAEC We further found that AR dramatically inhibited expression of Nox4 protein which gene silencing

mimicked the role of AR on the apoptosis under high glucose stimulation

CONCLUSION Taken together these results suggest that this Nox4-dependenet O2- production is critical for the apoptosis

of RAEC in high glucose AR has an anti-apoptosis role through Nox4-dependent O2- production which

address the vascular protective role of CPU0213 in diabetes mellitus

30

PP 4 Mutagenesis of precursor peptide for the generation of nosiheptide analogues

Shuzhen Wang1 Xulu Zheng1 Qi Pan1 and Yijun Chen1 1State Key Laboratory of Natural Medicines Laboratory of Chemical Biology China Pharmaceutical University Nanjing Peoples

Republic of China

OBJECTIVE Nosiheptide produced by Streptomyces actuosus ATCC 25421 is a typical thiopeptide biosynthesized

from a precursor peptide NosM by post-ribosomal peptide synthesis The objective of this study is to assess

which amino acid residue in the core peptide sequence of NosM is tolerated or restricted by the post-

translational machinery for nosiheptide towards the change of amino acid side chains

METHODS A gene-replacement approach was implemented in the nosM-deletion strain Site-directed mutagenesis of

five cysteine residues involved in the formation of thiazoles and the third threonine residue without

modification were carried out to generate ten NosM mutants with single residue replacement in the

chromosome

RESULTS The cysteine to serine mutations did not produce any predicted mature scaffolds The mutagenesis of the

third threonine residue with five different amino acids led to the maturation of three nosiheptide analogues

with neutral side chains retaining antimicrobial activities and no analogue production from two mutants with

charged side chains

CONCLUSION The cysteine to serine mutagenesis failed to produce any predicted mature scaffolds demonstrating that

these replacements are not accepted by the tailoring enzymes Three mutants of the third threonine residue

led to the maturation of nosiheptide analogues retaining antibacterial activities suggesting that the third

threonine residue in NosM is partially mutational residue for nosiheptide biosynthesis The present study

reveals the limitation and opportunity for nosiheptide-producing strain to generate nosiheptide analogues

and provides an insight into appropriate position and amino acid side chain for precursor peptide

engineering of nosiheptide biosynthesis

31

PP 5 The pharmacodynamic material basis and pharmacological mechanisms of Goutengsan in

treatment of type 2 diabetes induced cognitive and emotional disorders ZHAO He1 2 NIU Yi-min2SHAO Hua2 LIU Yang3

1） Department of Basic Medicine and Clinical Pharmacy China Pharmaceutical University Nanjing 210000

2） Department of Pharmacy Zhongda Hospital School of Medicine Southeast University Nanjing 210009China

3） College of pharmacyNanjing Medical UniversityNanjing 211166China

OBJECTIVE To establish analysis method for elucidating the constituents in vitro and in vivo of Gou teng san (Chotosan

CTS) to build the network relations between chemicals derived from CTS in vivo and endogenous

biomarkers selected from metabolomics and to clarify the pharmacodynamic material basis and

pharmacological mechanisms of Goutengsan in treatment of type 2 diabetes induced cognitive and

emotional disorders

METHODS By application of UPLC-QTOF-MS with the MSE acquisition mode we plan to separately characterize the

constituents of Goutengsan and the components in serum after oral administration of CTS By animal

behavior experiments western blot and immunochemistry to elucidate the pharmacological mechanisms

of CTS Metabolomic studies on the urine and plasma samples to obtain the endogeneous biomarkers in

this T2DM animal model and further to establish the relations between constituents from CTS and

endogeneous biomarkes by mathematic model and verify by in vitro experiments

RESULTS 1Pharmacological mechanisms Behavioral experiments verified that CTS could ameliorate cognitive and

emotional deficits in an animal model of type 2 diabetes and reverse the protein expression levels of

cholinergic system VEGFPDGF system and phosphorylated AktPKC in the hippocampus of this animal

model 2 Establish pharmacodynamic material basis By plotting of correlation between metabolomics

biomarkers and herabal-derived constituents in serum totoal six chemicals were discovered as

pharcodynamic material basis of CTS including isorhynchophyllic acid cimifugin 5-O-methylvisamminol

isocorynoxeine hirsuteine and hirsutine

CONCLUSION Through AktPKC signaling pathway CTS could protect cholinergic system and VEGFPDGF systems to

improve diabetes-induced cognitive and emotional disorders The pharcodynamic material basis of CTS

was characterized as isorhynchophyllic acid cimifugin 5-O-methylvisamminol isocorynoxeine hirsuteine

and hirsutine In this study we systematically elucidate the pharmacological mechanisms and

pharcodynamic material basis of CTS in treatment of type 2 diabetic induced cognitive and emotional

disorders which provide the basis of clinical application and deeper development of CTS

32


Liu WY1 Wang GJ1 Zhou F1 Zhang JW1

Key Laboratory of Drug Metabolism and Pharmacokinetics

State Key Laboratory of Natural Medicines

China Pharmaceutical University Nanjing China

OBJECTIVE Shenmai injection (SMI) is a Chinese patent-protected injection which was mainly made of Red Ginseng

and Radix Ophiopogonis Our study intended to verify the synergism of SMI to chemotherapeutics and

explore the underlying mechanism from PK-PD perspective

METHODS In vitro studies were based on LoVo colon cancer xenograft model Tumor growth was estimated by tumor

volume and weight HampE staining Ki67 staining and TUNEL assay were used to explore SMIrsquos influence

on antitumor effect of chemotherapeutics (Adriamycin Paclitaxel and 5-FU) Drug concentration in plasma

and tissues was determined by LC-MSMS Living two-photon imaging and immunostaining were used to

observe tumor vasculature In vivo studies were based on colon cancer cells Cytotoxicity was evaluated

by MTT assay cellular accumulation of drugs was examined by uptake assay subcellular distribution of

chemotherapeutics was determined by LC-MSMS and fluorescent dying PCR and western blots were

used to determine gene and protein expression expectedly

RESULTS SMI significantly increases the chemotherapy efficacy of chemotherapeutics while SMI itself has no obvious

anti-cancer effect SMI significantly increase drug concentration in tumor but never enhanced their side

effets to other important organs SMI significantly increased cytotoxicity and cellular accumulation of

chemotherapeutic drugs in colon cancer cells Also SMI remarkably increased drug distribution in target

organelles In addition we found that SMIrsquos synergistic effect was related to normalizing disorganized tumor

vascular system

CONCLUSION In summary our study suggested SMI enhanced the anti-cancer effects of chemotherapeutics in colon

cancers in vivo and vitro by improving pharmacokinetic behavior of the drugs at tissue-cell-subcell level

And the underlying mechanism was closely related to tumor vasculature modulation

33

PP 7 NAD biosynthesis combined with glucuronidation metabolism contributes to the detoxication of

berberrubine in kidney Na Yang Yuan Xie Jiye Aa Guangji Wang

Key Lab of Drug Metabolism and Pharmacokinetics Jiangsu Key Laboratory of Drug Design and Optimization China

Pharmaceutical University Nanjing China

OBJECTIVE Berberrubine (BRB) an active metabolite of berberine shows a high proportion of glucuronidation

metabolism in vivo This study aims to explore the underlying connection of the reduced glucuronidation

ability and inhibited NAD biosynthesis pathway with the nephrotoxicity induced by BRB in C57BL6J mice

fed with high fat diet (HFD)

METHODS C57BL6J mice fed with HFD were orally administered with BRB (50 mgkg) for 6 weeks A simultaneous

assay of BRB and its metabolite berberrubine-9-O-β-D-glucuronide (BRBG) was developed using LC-

MSMS to evaluate the pharmacokinetic profile of BRB in C57BL6J mice Hepatic and renal microsomes

were prepared to determine the UGT enzymes activity Compounds in NAD biosynthesis pathway in liver

and kidney were quantitatively analyzed HEK293 cell line was employed for investigating the toxic effect

of BRB in vitro and was transfected with UGTs to activate the glucuronidation process

RESULTS The ratio of BRBG to BRB in urine decreased significantly in C57BL6J mice fed with HFD compared with

normal diet while the plasma AUC0ndasht of both BRB and BRBG did not show any obvious change Biliary

excretion rate of BRBG was at a relative low level HFD slightly increased hepatic UGT activity but markedly

decreased renal UGT activity based on the incubation system of hepatic and renal microsomes The

decreased renal glucuronidation was accompanied with inhibited NAD biosynthesis pathway and related

metabolic disturbance in kidney which was not observed in liver The activation of glucuronidation in

HEK293 cells significantly increased the concentration of NAD Both glucuronidation activation and NAD

supplement could ameliorate BRB induced toxicity

CONCLUSION Glucuronidation is closely related with the NAD biosynthesis pathway which is involved in the functioning

of various enzymes and metabolic process The reduced renal glucuronidation ability combined with

inhibited NAD biosynthesis pathway highly contributes to BRB induced nephrotoxicity in C57BL6J mice on

HFD

34

PP 8 Curcumin attenuates liver fibrosis via suppression of succinate-associated HIF-1α induction in

HFD-fed mice Linlin She

Jiangsu Key Laboratory of Traditional Chinese Medicine Evaluation and Translational Research Department of Pharmacology of

Chinese Materia Medica China Pharmaceutical University 24 Tongjia Lane Nanjing 210009 China

OBJECTIVE Curcumin regulate the cross-talk between inflammation and fibrosis This study investigated the effect of

curcumin on liver fibrosis focusing on the regulation of metabolism

METHODS Mice fed a high-fat diet (HFD) showed induced liver fibrosis We observed the effects of curcumin on hepatic

fatty acid oxidation and hepatic stellate cells(HSCs) activation The cross-talk between inflammation and

fibrosis was also investigated in HSCs

RESULTS HFD-fed mice increased succinate accumulation due to the reversal of succinate dehydrogenase (SDH)

activation and induced NLRP3 inflammasome activation in a manner dependent on HIF-1α induction In

response to NLRP3 inflammasome activation the released IL-1β further increased TGF-β1 induction

suggesting the forward cycle between inflammation and fibrosis in HSCs activation In HFD-fed mice

curcumin inhibited hypoxic TGF-β1 induction and suppressed succinate-associated NLRP3 inflammasome

activation by inhibiting SDH activity and thereby prevented HSCs activation by blocking the cross-talk

between inflammation and fibrosis

CONCLUSION Succinate acted as a metabolic signal to enhance the liver fibrosis Curcumin reduced hepatic succinate

accumulation by combating fatty acid oxidation and attenuated inflammation by suppressing succinateHIF-

1α induction suggesting that succinate-associated HIF-1α induction in HSCs might be a therapeutic target

for the prevention of fibrosis in liver

35

PP 9 Liver injury of Dictamnine in mice and cytotoxicity of Dictamnine in wild-type and CYP3A4 over-

expressed cells revealed the role of CYP3A4 in induced-toxicity Zhuoqing Li Lingli Wang Huijun Li

State key Laboratory of Natural Medicines China Pharmaceutical University Nanjing China

OBJECTIVE To find out that whether Dictamnine (DTN) appeared to be hepatotoxic and illustrate the role of CYP3A4

enzyme in the toxicity in vitro

METHODS 35 male ICR mice were treated intragastrically with DTN at 40 80 160 320 mgkg in a solution of

DMSOPEFG200water (55045) and vehicle for a single dose To evaluate the liver injury serum alanine

aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured and liver

histopathological changes were observed Further IC50 values were used to assess the different cytotoxicity

of DTN and Diclofenac sodium (positive control) in primary human hepatocytes (PHH) HepG2 L02 and

NIH3T3 cells respectively Stable CYP3A4 over-expressed L02 and HepG2 cells were constructed and

validated Inhibitor and inducer of CYP3A4 enzyme were pre-incubated with PHH to compare the cell

viability meanwhile comparison between wild-type and over-expressed HepG2 and L02 were also

conducted

RESULTS Increased ALT level and injured liver tissue section showed that DTN displayed hepatotoxicity in mice DTN

exhibited inhibition effects on various cell lines with different IC50 values (PHHlt L02lt HepG2lt NIH3T3)

showing no cytotoxicity in NIH3T3 cells DTN inhibited cell growth of HepG2 and L02 cells in a dose-and

time-dependent manner and CYP3A4 inhibitor and inducer modulated toxicity in the HPP Cytotoxicity of

DTN was enhanced in CYP3A4 over-expressing L02 and HepG2 cells which suggested that CYP3A4

enzymes are implicated in DTN induced cytotoxicity

CONCLUSION We found that Dictamnine presented liver injury effect in mice for the first time and CYP3A4 enzyme has

an induced action on the toxicity in vitro

36

PP 10 Mechanisms of dronedarone-induced cardiac adverse exacerbation Role of mitochondrial

toxicity A Karkhanis1 ECY Chan1


OBJECTIVE

Dronedarone is a novel anti-arrhythmic drug approved for the treatment of paroxysmal and persistent atrial

fibrillation (AF) While dronedarone is safer than amiodarone in terms of systemic toxicities it has been

implicated in worsening cardiac failure in AF patients with NYHA Class IV heart failure with unknown

mechanism1 It has been shown that dronedarone causes mitochondrial toxicity and oxidative stress in

HepG2 hepatocytes2 thus explaining its clinical hepatotoxicity3 In this project we have investigated

whether dronedarone would cause mitochondrial damage in cardiomyocytes

METHODS

Differentiated rat cardiomyocyte H9c2 cells were treated with dronedarone amiodarone and their

respective metabolites namely N-desbutyldronedarone (NDBD) and N-desethylamiodarone (NDEA)

Biochemical endpoints such as intracellular ATP content cytosolic and mitochondrial superoxide dismutase

(SOD) activity mitochondrial membrane potential (MMP) and carnitine palmitoyltransferase I activity (CPT1)

inhibition were measured Inhibition of mitochondrial complex I and II was studied in isolated rat heart

mitochondria

RESULTS

Dronedarone amiodarone NDBD and NDEA caused significant decrease in intracellular ATP content

(IC50=049 184 107 063 microM) respectively and MMP was potently perturbed (IC50=05 294 128 738

microM) respectively Similarly complex I activity was inhibited by dronedarone (IC50=307 microM) and amiodarone

(IC50=524 microM) but not NDBD and NDEA Dronedarone NDBD and NDEA weakly inhibited CPT1 activity

(IC50=40 10 10 microM) respectively while amiodarone (IC50gt100 microM) did not show any inhibition at

physiological concentrations Cytotoxicity ATP decrease MMP disruption was ameliorated by exogenous

pre-treatment of 1112-EET and 1415-EET

CONCLUSION

We conclude that dronedarone causes significant mitochondrial damage as compared to amiodarone by

perturbing mitochondrial membrane potential and inhibition of mitochondrial complex I activity and not CPT1

inhibition We postulate that mitochondrial damage to the

37

PP 11 Perturbation of arachidonic acid metabolism by antiarrhythmic drugs Implications in cardiac

safety A Karkhanis1 N Tram ECY Chan1


OBJECTIVE

Amiodarone is most widely prescribed anti-arrhythmic drug but is associated with severe systemic toxicity

Dronedarone a non-iodinated benzofuran analogue of amiodarone causes cardiac failure exacerbation1

Catalytic enzymes like CYP2J2 metabolize arachidonic acid (AA) to cardioprotective epoxyeicosatrienoic

acids (EETs) which are in turn systemically cleared by soluble epoxide hydrolase (sEH)2 We postulate that

perturbation of AA metabolism due to dronedarone and not amiodarone may explain its cardiovascular

adverse effects

METHODS

In this project firstly we examined whether dronedarone amiodarone and their active metabolites N-

desbutyldronedarone (NDBD) and N-desethylamiodarone (NDEA) respectively inhibit recombinant

CYP2J2 using arachidonic acid as substrate Secondly we tested the inhibitory effect on recombinant

human sEH (rHsEH) using 1415-EET as probe substrate Finally we developed a sequential metabolism

model to predict the effect of sEH and CYP2J2 dual inhibition on the fold-change in 1415-EET level

(119862$119862)

RESULTS

We found that dronedarone (Ki=042 microM) amiodarone (Ki=063 microM) NDBD (Ki=458 microM) inhibit rCYP2J2-

mediated arachidonic acid metabolism Moreover we found that dronedarone (Ki=2194 microM) amiodarone

(Ki=233 microM) NDBD (Ki=116 microM) and NDEA (Ki=188 microM) also inhibit rHsEH The sequential metabolism

model predicted that dronedarone (119862$119862=017) and amiodarone (119862$119862=028) leads to a decrease

in overall 1415-EET level while NDBD (119862$119862=231) and NDEA (119862$119862gt355) would significantly

elevate intracardiac 1415-EET

CONCLUSION

We conclude that dronedarone inhibits CYP2J2 while amiodarone inhibits sEH potently We postulate that

the net decrease in cardioprotective EETs may contribute to the cardiac failure exacerbation in dronedarone

therapy

38

PP 12 Quantification of desoxyrhapontigenin (4-methoxyresveratrol) in rat plasma by LCMSndashMS

Application to pre-clinical pharmacokinetic study Yu Dai Amanda Li Cheng Tan Huan Chen Hai-Shu Lin

Department of Pharmacy National University of Singapore 10 Kent Ridge Crescent Singapore 119260

OBJECTIVE Desoxyrhapontigenin (DRG 4rsquo-methoxyresveratrol or trans-35-dihydroxy-4rsquo-methoxystilbene) is a

monomethyl ether derivative of resveratrol that can be found in rhubarb a remedy widely used in oriental

herbal medicines Similar to resveratrol (RES) DRG possesses versatile pharmacological activities

Pharmacokinetic study plays a significant role in drug development and discovery In this study it is aimed

to study the pharmacokinetic profile of DRG in rats to facilitate further exploration on DRG Also a sensitive

and reliable liquid chromatographyndashtandem mass spectrometry(LC-MSMS) method will be developed and

validated for quantifying DRG in rat plasma

METHODS Protein precipitation method was used to prepare the plasma sample the electrospray ionization was

operated in negative ion mode while DRG and 13C-trans-stilbene (internal standard) were measured by

multiple reactions monitoring (DRG mz 257rarr 185 13C-trans-stilbene 2331rarr 191)

RESULTS This LCndashMSMS method displayed good selectivity sensitivity (lower limit of quantification = 25 ngmL)

accuracy (both intra- and inter-day analytical recovery within 100 plusmn 10) and precision (both intra- and

inter-day RSD lt10) The matrix effect was found to be insignificant The pharmacokinetic profiles of DRG

were subsequently examined in Sprague-Dawley rats Upon intravenous injection (4 mgkg or 10 mgkg)

DRG had serious rapid clearance (30893 plusmn 4863 or 23452 plusmn 663 mLminkg) and extremely short mean

transit time (MTT = 1132 plusmn 405 or 941 plusmn 050 min) After oral dosing (10mgkg or 50 mgkg) the

bioavailability of DRG was limited (F = 1014 plusmn 492 or 2244 plusmn 522) Also an obvious enteric-hepatic

circulation phenomenon was observed

CONCLUSION Pharmacokinetic study of DRG suggested it may be associated with metabolic instability and subsequently

suffer from serious rapid clearance and low oral bioavailability Also DRG may obtain the ability to saturate

metabolic process The information obtained from this study will facilitate further medicinal exploration on

DRG and other RES analogues

39

PP 13 Inhibition of 5-lipoxygenase prevents lipopolysaccharide induced depressive-like behaviors and

neuroinflammation in mice Dandan Li Hang Xie

Department of Pharmacology Key Laboratory of Neuropsychiatric Diseases China Pharmaceutical University Nanjing 210009

OBJECTIVE Numerous studies have demonstrated that neuroinflammation is recognized as one of the important

hallmarks of depression 5-lipoxygenase (5LO) was reported to be involved in neuroinflammation and

participated in several types of central nervous system (CNS) damage but the role of 5LO in the

pathogenesis of depression remains unknown

METHODS We investigated the effects of zileuton a 5LO inhibitor on depressive behaviors using tail suspension test

(TST) forced swimming test (FST) and novelty-suppressed feeding test (NSFT) in mice exposed to LPS

injection The activation of microglial cells and NF-κB p65 TNF-α IL-1β brain-derived neurotrophic factor

(BDNF) the c-AMP response element-binding protein (CREB) were determined in mouse hippocampus

RESULTS We firstly found that the expression of hippocampal 5LO was gradually increased over LPS exposure and

was reversed by fluoxetine administration Zileuton significantly suppressed LPS-induced depressive

behaviors evidenced by the decreases in immobility time in TST and FST as well as the latency to feed in

NSFT Zileuton also alleviated LPS-induced neuroinflammation characterized by prevention the activation

of microglia down-regulated the NF-κB p65 TNF-α and IL-1β increased the p-CREBCREB and

mBDNFproBDNF ratio in hippocampus

CONCLUSION Hippocampal 5LO participates in depression and 5LO inhibitor prevents LPS-induced depressive-like

behaviors and neuroinflammation suggesting that suppression of 5LO could prevent the development of

depression

40

PP 14 Protective effect of Icariin on renal function in rats with type 2 diabetic nephropathy induced by

HFD combined with low dose STZ via insulin signaling pathway Qin Wang1 Xuan-Sheng Ding2


OBJECTIVE To investigate the ameliorative effect of icariin on renal function in rats with type 2 diabetic nephropathy

and the potential mechanism of regulating the abnormality of insulin signal pathway in renal tissue

METHODS Diabetes was induced in male SD rats by an intraperitoneal injection of STZ (40 mgkg ip) Sustained

blood glucose levels (gt167 mmoll) were considered as diabetic and for subsequent study 100 rats were

randomly divided into 5 groups control diabetic diabetic + Icariin (60 mgkg ig) diabetic + Icariin

(120 mgkg ig) diabetic + Metformin (200 mgkg ig) administered 12 weeks from 9th to 21th week

Research was carried out at the beginning of 22th week All rats were anaesthetized and then killed to

remove kidneys Blood glucose serum creatinine (Cr) serum free fatty acid (FFAs) total cholesterol (TC)

serum triglyceride (TG) blood urea nitrogen (BUN) LDL-c and HDL-c in the kidney tissue were measured

Glomerular podocyte morphology was observed by light microscopy Western blot was employed to

determine the proteins levels of Nephrin Desmin PI3K-P85 and type IV collagen

RESULTS The enhancement of blood glucose Cr FFAs TC TG BUN and LDL-c was found in model group which

was significantly attenuated by Icariin The renal pathological changes in Icariin treatment group were

ameliorated Meanwhile decreased the expression of Nephrin protein level as well as elevated Desmin and

collagen IV levels in renal tissue were significantly reversed by Icariin Furthermore the Icariin normalized

insulin and PI3K-Akt signaling

CONCLUSION Icariin can evidently relieve renal damage in rats with diabetic nephropathy which might be related to

modulating the lipid metabolism and insulin resistance

41


Lin WuYF Chan

School of International Pharmaceutical Business China Pharmaceutical University Nanjing China

OBJECTIVE Pharmaceutical Care adheres to patient care and patient-centered The works of hospital pharmacists is

still stuck in the transition period from traditional to clinical in China most PC is in the propaganda level

This paper aims to identify factors to influence pharmacistsrsquo PC behavior and to explore diverse views

related to clinical pharmacy service of demographic groups and in various regions using the TPBampTAM

model

METHODS The surveys were conducted between January and May 2016 in twenty-seven representative cities and

main respondents were clinical pharmaceutical staff in the third-class hospitals The overall perspective

assumption model was built by using TPBampTAM theory the factor and path of Chinese pharmacists based

on SEM theory Subdivision based on TTM we combined it with the above point hypothesis model and

analyze the influencing factors of behavior at different stages

RESULTS Based on 646 valid questionnaires the results show that clinical pharmacy service intention and behavior

could be predicted by five significant factors pharmacistsrsquo attitudes toward their mission a sense of

achievement recognition from others perceived behavioral control in the mastery of professional

knowledge and their experience of past behavior Additionally the study found that in order to make PC

intention and behavior more effectively a step-by-step problem solving method is highly helpful and efficient

From the perspective of improving the frequency attitude is significant during no considering stage to

considering stage and the past behavior is significant in the action stage Attitude past behavior perceived

behavioral control is significant in improving the participation and enhancing the level of factors from no

considering stage to considering stage and the maintenance stage

CONCLUSION These findings can assist government and policy makers to promote the service level of pharmacistsrsquo PC

and also guide the hospital to enhance the professional knowledge and skills training of pharmacists fulfill

clinical pharmacistsrsquo self-actualization needs is always meaningful

42

PP 16 Redox-responsive supramolecular hydrogel based on 10-hydroxy camptothecin-peptide covalent

conjugates with high loading capacity for drug delivery C Wu a RX Li a YJ Yin a WY Zhong a b

a Department of Analytical Chemistry China Pharmaceutical University Nanjing 210009 Peoplersquos Republic of China b Key Laboratory of Biomedical Functional Materials China Pharmaceutical University Nanjing 210009 Peoplersquos Republic of China

OBJECTIVE In order to improve the water solubility and anticancer effect of 10-hydroxy camptothecin (HCPT) a redox-

responsive supramolecular hydrogel system was constructed

METHODS We used the combined method of solid phase synthesis and chemical synthesis to make drug conjugate

with peptide then the hydrogel was formed by cleaving disulfide bond by glutathione (GSH)

RESULTS The combination of hydrophobic HCPT with hydrogel was a simple and effective way to improve the

solubility of HCPT and the drug loading capacity of delivery system The transmission electron microscopy

(TEM) image revealed the self-assembled hydrogel was long and thin nanofibers with a width of less than

10 nm Rheological test verified the hydrogel had fine physical properties In vitro release experiment

showed that the accumulative releasing percentages within 72 h of HCPT-peptide hydrogels at 30 40

50 were 168 213 and 268 respectively which indicated the HCPT-peptide hydrogels had a

significantly sustained-release characteristic Besides in vitro anticancer assay showed that HCPT-peptide

hydrogels possessed a favorable anticancer efficacy

CONCLUSION These results indicated that HCPT-peptide hydrogel had great potential for cancer treatment as a novel

injectable drug delivery system

43

PP 17 Mesoporous silica nanoparticles incorporated hybrid monolithic stationary phase immobilized

with pepsin for enantioseparation by capillary electrochromatography Shujuan Xu 1 Rongzhen Mo2 Yibing Ji3

Department of Analytical Chemistry China Pharmaceutical University Nanjing 210009 China

OBJECTIVE The need for fast and effective analytical tools for chiral separations is continuously growing because that

understanding the pharmacodynamic and pharmacokinetic effects of single enantiomer on the metabolism

of chiral drugs is key for the development of novel drugs For the purpose of obtaining a rapid efficient and

stable enantiomeric separation media we fabricated a novel mesoporous silica nanoparticles incorporated

chiral hybrid monolithic stationary phase

METHODS The stationary phase was firstly prepared by an in situ copolymerization of amino-modified mesoporous

silica nanoparticles (NH2-MSN) glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) and

then functionalized with pepsin as chiral selector The column was successfully employed for

enantioseparation of fifteen basic chiral drugs in capillary electrochromatography(CEC) Effects of

nanoparticles percentage pepsin concentration and electrochromatography conditions were investigated

RESULTS Incorporation of NH2-MSN into monolithic column greatly increased the interactions between the tested

analytes and the stationary phase and significantly improved their CEC separation All the analytes could

be eluted in less than ten minutes and nine of them could achieve baseline separation Moreover some

enantiomers could be completely separated as fast as in 3 minutes Satisfactory repeatabilities with relative

standard deviations less than 42 were achieved through intraday interday column-to-column and batch-

to-batch investigations

CONCLUSION The prepared NH2-MSN incorporated monolithic column gives excellent performance for CEC separation

of chiral compounds The simultaneous utilization of the unique properties of mesoporous silica

nanoparticles and versatile features of monoliths could be a promising strategy for enantioseparation

44


Fangrong Zhang Min Li Yujie Su Jianping Zhou Wei Wang

State Key Laboratory of Natural Medicines Department of Pharmaceutics China Pharmaceutical University 24 Tongjiaxiang

Nanjing 210009 China

OBJECTIVE Regulation of gene expression using p53 is a promising strategy for treatment of numerous cancers and

chemotherapeutic drug dichloroacetate (DCA) induces apoptosis and growth inhibition in tumor without

apparent toxicity in normal tissues Combining DCA and p53 gene could be an effective way to treat tumors

The progress towards broad applications of DCAp53 combination requires the development of safe and

efficient vectors that target to specific cells In this study we developed a DSPE-PEG-AA (12-distearoryl-

sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol-2000)] ammonium salt-anisamide)

modified reconstituted high-density lipoprotein-based DCAp53-loaded nanoparticles (DSPE-PEG-

AArHDLDCA-PEIp53 complexes) which was fabricated as a druggene dual-targeting co-delivery system

for potential cancer therapy

METHODS DCA-PEI was conjuncted and utilized to effectively condense the p53 plasmid and incorporate the plasmid

into rHDL to form rHDLDCA-PEIp53 nanoparticles Modify DSPE-PEG-AA into the surface of phospholipid

thus producing DSPE-PEG-AA rHDLDCA-PEIp53 nanoparticles which was shown as a promising

antitumor system for cancer therapy

RESULTS The DSPE-PEG-AArHDLDCA-PEIp53 complexes exhibited desirable and homogenous particle size

neutral surface charge and low cytotoxicity for normal cells in vitro The results of confocal laser scanning

microscopy(CLSM) and flow cytometry confirmed that the scavenger receptor class B type I (SR-BI) and

sigma receptor mediated dual-targeting function of the complexes inducing efficient cytoplasmic drug

delivery and gene transfection in human lung adenocarcinoma cell line A549 And in vivo investigation on

nude mice bearing A549 tumor xenografts revealed that DSPE-PEG-AArHDLDCA-PEIp53 complexes

possessed specific tumor targeting and strong antitumor activity

CONCLUSION The work described here demonstrated that the DSPE-PEG-AArHDLDCA-PEIp53 complexes might offer

a promising tool for effective cancer therapy

45

PP 19 The unit-dose plate for compounding orodispersible films in the pharmacy ndash

a human factors evaluation Wen Chin FOO1 Alethea Xinyi LIM1 Han Hui CHEONG2 Sui Yung CHAN1

1 Department of Pharmacy National University of Singapore 2 KK Womenrsquos and Childrenrsquos Hospital Singapore

OBJECTIVE Extemporaneous oral preparations are routinely compounded in the pharmacy to address the needs of

special populations Recently orodispersible films (ODF) have demonstrated clinical potential as a suitable

dosage form for pharmacy preparations The aim of this study was to evaluate the ease of compounding

ODFs using a simple and inexpensive method developed with the unit-dose (UD) plate

METHODS The conventional method of preparing ODFs employs solvent-casting using a film applicator which is

associated with content uniformity issues To address this issue we developed the UD plate to circumvent

the effect of viscosity and operator manipulation on content uniformity A human factors evaluation was

performed by employing usability testing techniques including non-participant observation and post-test

interviews Eight final-year Pharmacy undergraduate students were recruited by convenient sampling

Participants were required to perform four tasks according to given instructions which constitutes the

workflow for compounding ODFs

RESULTS All participants completed the tasks successfully without safety-related incidents An average of 90 of

ODFs prepared by each participant were considered acceptable as defined as films which were free of

imperfections and without spillage Calibration error during preparation of the drug solution was in the range

of 0 ndash 3 which was considered acceptable Feedback from participants was that the instructions provided

were clear and easy to understand Participants found all four tasks easy to perform with scores of 4 or 5

upon a total score of 5 in terms of ease of performing the task Suggestions from participants for

improvements in the compounding method and instructions were also recorded

CONCLUSION ODFs can be safely and easily compounded using the UD plate by final-year undergraduate Pharmacy

students Results from the usability test and participant feedback will be used to further refine the

compounding process to improve usability and reduce the risk for errors

46

PP 20 Nanoparticles of a novel polyethoxy-based polymer synthesized by microwave assisted

polymerization CXY Wong1 W Fong1 CH Soh2 WK Chui1 FS Chia2 GNC Chiu1

1Department of Pharmacy Faculty of Science National University of Singapore Singapore 2School of Chemical and Life Sciences Singapore Polytechnic Singapore

OBJECTIVE To explore the feasibility of preparing nanoparticles from a novel polyethoxy-based polymer to be used as

a drug delivery system

METHODS A novel polymer series was synthesized using a one-pot microwave-assisted condensation reaction

whereby different alkyl chain lengths (C1 C10 C12 and C16) were used in the side chain of the polymer

Blank nanoparticles were prepared via self-assembly or thin film hydration method Particle size was

measured to assess the physical stability of the nanoparticles To assess the toxicity potential of the

nanoparticles viability of MRC-5 cells was assessed 72 hours after treatment with blank nanoparticles

RESULTS Nanoparticles were prepared from the polymer series via self-assembly or the thin film hydration method

In the series of polymers only the polymer with alkyl side chain length of 1 carbon produced self-assembled

nanoparticles while polymers with alkyl side chain length of 10 12 and 16 carbon formed nanoparticles

with the thin film hydration method Particle size of blank nanoparticles prepared from polymers with

different alkyl chain lengths of 1 10 12 and 16 were smaller than 250 nm Nanoparticles largely exhibited

a negative zeta potential of about -20 mV with exception of self-assembled nanoparticles from polymer C1

(~3 mV) On storage at room temperature over 6 weeks nanoparticles of polymers C1 and C10 remained

physically stable with polydispersity index (PDI) below 02 while significant increases in particle size and

PDI were observed with the other polymers Cell viability of MRC-5 after treatment with blank nanoparticles

for 72 hours was 791 164 64 and 614 for C1 C10 C12 and C16 polymers respectively

CONCLUSION Nanoparticles of polymer C1 formed via self-assembly appears to be promising as a drug carrier as they

are stable at room temperature over 6 weeks and are relatively non-cytotoxic Further work can be done to

optimize polymer C1 as a nanoparticulate drug delivery system

47

PP 21 Dissolvable Microneedles for Transdermal Immunotherapy

Himanshu Kathuria1 Dennis Lim1 Kang Lifeng2

1 Department of Pharmacy National University of Singapore Singapore 117543

2 Faculty of Pharmacy University of Sydney Email lifengkangsydneyeduau

OBJECTIVE The prevalence of atopic diseases such as asthma atopic dermatitis and allergic rhinitis continues to rise

in the last few decades Transdermal immunotherapy can potentially be utilized to eradicate or fully treat

an atopic disease However the delivery of allergen to desensitize the skin has been limited due to the

tough skin barrier The dissolving microneedle could potentially be utilized to deliver the allergen by creating

micro punctures The aim of this study is to develop faster fabrication method of polymeric MN array to

deliver the drugs through skin

METHODS In the study Poly-vinyl-pyrrolidone (PVP) based slow dissolvable microneedle (SDMN) patches have been

fabricated through a novel photolithographic approach which allowed a short fabrication process time and

gentle fabrication conditions The various dissolution modifiers have been investigated to assess the effect

on dissolution delay to form SDMN The SDMN patches were characterized for mechanical strength

moisture absorption dissolution and penetration efficiency

RESULTS All the SDMN patches were able to penetrate the physical stratum corneum barrier The fabricated

microneedles had distinct dissolution profiles ranging from 45 min to 48 h They were categorized into four

categories namely rapidly dissolving moderately slow dissolving slow dissolving and very slow dissolving

microneedles Additionally the SDMN retained sufficient and appropriate functional parameters upon the

incorporation of dissolution modifiers Tofacitinib and peanut protein

CONCLUSION The faster method of fabrication has been developed using photolithography approach The fabricated

SDMN patches have the potential for flexible applications depending on the dissolution profile needs for

transdermal immunotherapy and skin applications

48

PP 22 The influence of lipid modifiers on the rheological properties of molten paraffin wax and the

degree of drug coating in resultant spray-congealed microparticles H Ouyang1 A Y Zheng1 P W S Heng1 L W Chan1

1Department of Pharmacy National University of Singapore

OBJECTIVE Spray congealing is suitable for the production of specialized drug delivery systems using different matrix

materials The use of hydrophobic materials has not been well explored Fischer-Tropsch paraffin wax

which is chemically well-defined is potentially useful for the production of spray-congealed microparticles

for sustained release taste masking or stability enhancement of drug However it was found that the drug

particle was not entirely coated by the paraffin wax This study aimed to develop drug-loaded paraffin wax

microparticles by spray congealing and to investigate the effects of lipid modifiers

METHODS The effects of lipid modifiers (stearic acid cetyl alcohol and cetyl esters) and model drug (paracetamol) on

the rheological properties of paraffin wax were investigated by continuous ramping tests Selected

formulations were spray-congealed and the resultant microparticles characterized The microparticle size

was determined using optical microscopy and drug assayed by UV spectrophotometry The degree of drug

coating and drug release were investigated by Raman spectroscopy and dissolution studies respectively

RESULTS Molten paraffin wax exhibited Newtonian flow which was transformed to plastic flow by the addition of

paracetamol (20) and pseudoplastic flow by the lipid modifier (40) The viscosity was decreased by the

lipid modifier producing a blend that was more amenable for spray congealing Paraffin wax produced

spray-congealed microparticles with sustained drug release high drug encapsulation efficiency but the drug

particles were not entirely coated Addition of lipid modifier resulted in smaller microparticles Drug

encapsulation efficiency remained high Degree of drug coating and drug release were also higher due to

lower hydrophobicity of the resultant matrix The different lipid modifiers showed varying impact

CONCLUSION Paraffin wax and blends are suitable matrix materials for the production of spray-congealed microparticles

The lipid modifiers altered the rheological properties and hydrophobicity of the molten paraffin wax and are

useful for modifying the microparticle properties

49

PP 23 Orally-Dissolving Films for the Delivery of Ropinirole

B Zhang Y Fang H Han K L Lai Q Li S Zhang H Y Li T N Lam W Y Lee

School of Pharmacy The Chinese University of Hong Kong OBJECTIVE Parkinsonrsquos disease (PD) is a major threat to aging population and caused by loss of dopaminergic neurons

Ropinirole a non-ergoline type dopamine receptor agonist has been widely used as it safe and can protect

brain tissue from oxidative injury However there is a pressing need to devise formulations that allow easy

administration and fast drug action in consideration of first-pass metabolism and the ldquooff-periodrdquo of PD

patient In this study we aimed at developing ropinirole orally-dissolving films of and assessing their in vivo

pharmacokinetic performance after sublingual or buccal administration

METHODS Ropinirole oral film (ROF) was prepared by solvent-casting method and drug content disintegration

dissolution etc were characterized In vitro toxicity was determined by LDH release assay Finally the

pharmacokinetic studies were conducted by three different routes (ie oral sublingual and buccal) of

administration in rabbits

RESULTS ROF appeared to be off-white with opacity It started disintegrating within 10 seconds and complete

released within 2 minute ROF were shown to be physically stable for at least 28 days under three standard

ICH conditions Compared with the original LDH level both ropinirole and ROF did not trigger significant

LDH release after 4-hour incubation with sublingual tissues which indicated ROF didnrsquot cause any tissue

damage After buccal or sublingual administration of ROF ropinirole could be detected in the plasma within

15 minutes at 40 to 50 ngmL which showed a fast drug absorption into the systemic circulation Relative

to oral route of administration sublingually and buccally applied ROF increased the AUC0rarr6hour by about 7x

which may attribute to the avoidance of first-pass metabolism

CONCLUSION We have developed ROF with improved bioavailability in vivo after sublingual or buccal administration This

formulation can potentially overcome the biopharmaceutical challenges of ropinirole and provide a

convenient method of anti-PD drugs administration

50

Acknowledgments We wish to thank the following people for their support and contributions

12th PharmSciAsia Organizing Committee The Chinese University of Hong Kong

Faculty Prof Zuo Zhong Joan

Prof Lee Hon-leung Vincent

AProf Lee Wing-yan Vivian

AProf To Kin-wah Kenneth

AsstProf Lee Wai Yip Thomas

AsstProf Lam Tai-ning Teddy

Administrative staff Rose Chan

Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo

AAPS-CUHK Student Chapter

Chairperson Zhang Bowen Cathy

Chairperson elect Ren Tianjiang Kiko

Treasurer Qian Chen Yu Syrita

Vice chairpersons Hui Ka Ho Matthew

Hung Yu Candy

Secretary Wang Qianwen Chores

Membership officer Sze Lai Bun Justin

Members Chan Pui Shan Emily

Li qingqing

Jiang Minghuan Henry

The National University of Singapore

Faculty Prof Chai Li Lin Christina (HoD)

Prof Ho Chi Lui Paul

AProf Chew Eng Hui

Asst Prof Lin Haishu

51

Asst Prof Wee Hwee Lin

Administrative staff Chew Ying Ying

Liew Kai Wei Adreana

Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly

AAPS-NUS Student Chapter

Faculty advisor Dr Ong Pei Shi

Chairperson Foo Wen Chin

Vice-chairperson Wong Xin Yi Cheryl

Secretary Himanshu Kathuria

Treasurer Ouyang Hongyi

Project coordinator Mohua Das

Immediate past chairperson Hiew Tze Ning

ALL STAFF IN SCHOOL OF PHARMACY THE CHINESE UNIVERSITY OF HONG KONG ALL STAFF IN DEPARTMENT OF PHARMACY THE NATIONAL UNIVERSITY OF SINGAPORE

52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

Day 1CUHK SUMMER WORKSHOP

for Introducing Pharmacy Research Postgraduate Programmes amp HKPFS Scheme~middot ~ Pursuing A Rewarding Career In Research ~middot ~

4 July 2017 (Tuesday) ︳0930AM ndash 500PMLecture Theatre G02 Lo Kwee-Seong Integrated Biomedical Sciences Building (LKIBSB) Area 39 CUHK

Organized by School of Pharmacy Faculty of Medicine The Chinese University of Hong Kong

Date Time Event Presenter4 July 2017 (Tuesday)

0930 ndash 1000 Registration1000 ndash 1005 Opening Remarks Prof Joan ZUO

Director and Professor School of Pharmacy CUHK

1005 ndash 1100 Launching Your Research CareerHighlight - How to develop a career as a researcher - Career opportunities in the research field

Prof Vincent LEEResearch ProfessorSchool of Pharmacy CUHK

1100 ndash 1110 Group Photo Session amp Break

1110 ndash 1210 Pharmacy Research at CUHK CUHK Pharmacy Professors

1210 ndash 1400 Lunch with CUHK Pharmacy Professors and Current Pharmacy Postgraduate Students Highlight - Experience sharing and discussion

CUHK Pharmacy Professors amp Current Pharmacy Postgraduate Students

1415 ndash 1515 Study at CUHKHighlight - Introducing the School of Pharmacy - Introducing Research Postgraduate Programmes- HKPFS studentships travel grants amp scholarships- Overseas Conferences - Admission procedure


1515 ndash 1530 AAPS-CUHK Students Chapter Presentation Ms Cathy ZHANGChairpersonAAPS-CUHK Student Chapter

1530 ndash 1545 Closing AddressSouvenir Presentation


1545 ndash 1700 School Lab Tour

Symposium Preparation

Current Pharmacy Postgraduate Students

Symposiumrsquos Poster Participants

1600 1630 1700

Shuttle Bus Time Schedule

Area 39 MTR Station













































Area 39 MTR Station

1

Faculty speaker 1


Assistant Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 6537

Fax (+65) 6779 1554

Email phalhnusedusg










2

Faculty speaker 2



Associate Professor





Tel (+852) 3943 8012

Fax (+852) 2603 5295















3















4

Faculty speaker 3





18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554
















5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4













































Area 39 MTR Station

1

Faculty speaker 1


Assistant Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 6537

Fax (+65) 6779 1554

Email phalhnusedusg










2

Faculty speaker 2



Associate Professor





Tel (+852) 3943 8012

Fax (+852) 2603 5295















3















4

Faculty speaker 3





18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554
















5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

1

Faculty speaker 1


Assistant Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 6537

Fax (+65) 6779 1554

Email phalhnusedusg










2

Faculty speaker 2



Associate Professor





Tel (+852) 3943 8012

Fax (+852) 2603 5295















3















4

Faculty speaker 3





18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554
















5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

2

Faculty speaker 2



Associate Professor





Tel (+852) 3943 8012

Fax (+852) 2603 5295















3















4

Faculty speaker 3





18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554
















5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

3















4

Faculty speaker 3





18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554
















5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

4

Faculty speaker 3





18 Science Drive 4

Singapore 117543

Tel (+65) 6516 5530

Fax (+65) 6779 1554
















5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

5

Faculty speaker 4





Tel (+852) 3943 9795

Fax (+852) 2603 5295








6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

6


Lecturer




18 Science Drive 4

Singapore 117543

Tel (+65) 6601 1236

Fax (+65) 6779 1554



Cancer Treatment













7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

7

Faculty speaker 6


Associate Professor




18 Science Drive 4

Singapore 117543

Tel (+65) 6516 1995

Fax (+65) 6779 1554













8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

8





Transport Q Wang

OP 3



Verapamil

Z Qiao











OP 12



Receptor Class A

H Y Li





OP 15



cancer cells

Y Wang


9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

9




X Zang


XY Zhang


J Su



H Zhao


WY Liu


Y Na


L She


Z Li


A Karkhanis


A Karkhanis


Y Dai


D Li



L Wu


C Wu


S Xu

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

10


F Zhang


W C Foo




H Ouyang


Map to the canteen

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

11


















development

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

12






























13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

13


























14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

14

OP 4





Singapore 13864
















15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

15

OP 5





with AF


















16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

16


K H Hui T N Lam




















prediction model




17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

17














confocal microscopy












wrinkles

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

18










underline mechanism


















19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

19


H L Wee













20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

20


W Y Lee



vitreoretinopathy















21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

21


TN Hiew PWS Heng





















22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

22







angiogenesis





















23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

23























24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

24














functional studies
















25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

25




China


















26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

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Slide Number 2

Slide Number 3

Slide Number 4

26


EH Chew

























outcomes

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























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48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

27






investigated
















28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

28


X Y Zhang1 F Zhao2












channel



29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

29

















30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

30



Republic of China








chromosome




charged side chains








31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

31










emotional disorders




















32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

32






















vascular system




33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

33


























HFD

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

34





















35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

35














conducted









36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

36




OBJECTIVE







METHODS






mitochondria

RESULTS








CONCLUSION




37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

37




OBJECTIVE






adverse effects

METHODS






(119862$119862)

RESULTS







CONCLUSION



therapy

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

38


























39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

39




















depression

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

40






















41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

41


Lin WuYF Chan






model


















42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

42


















43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

43























44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

44



























45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

45

























46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

46
























47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

47
























48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

48

























49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

49
























50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

50










Kate Cheung

Echo Mok

Karen Tsoi

Joseph Suen

Bobby Cheng

Cedric Lo






Hung Yu Candy




Li qingqing





AProf Chew Eng Hui


51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

51




Napsiah Bte Suyod

Tan Shuyun

Law Siew Chin

Chan Wei Ling Kelly










52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

52

Our Sponsors

Slide Number 2

Slide Number 3

Slide Number 4

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