Oregon Institute of Occupational Health

Sciences Laboratory Safety Training

Overview

This training segment describes:

•  The Laboratory Safety Manual •  Safety Data Sheets (SDSs) •  Protective Clothing •  Lab Rules and Procedures •  Emergency Information

Quiz Questions

There are 10 quiz questions presented in

the course of this training exercise. Please complete the score sheet provided

with this training course and return to Janice Stewart (Room 2501,

4-2516)

Occupational Health Science’s Safety Policy

•  Safety is our number one priority.

•  We follow OHSU and Oregon OSHA rules on safety.

•  The rules are based on state and federal OSHA standards for Occupational Exposure to Hazardous Chemicals in Laboratories (29 CFR 1910.1450).

•  If you have any safety-related questions, ask your supervisor or Dede Montgomery, chair of the CROET Safety Committee at montgomd@ohsu.edu.

The Laboratory Safety Manual

OHSU Laboratory Safety Manual •  The Laboratory Safety Manual is

OHSU’s major source for safety information.

•  You can access the a copy of the

manual here. Hard copies are available for check out in our main office on the 2nd floor.

•  The manual serves as OHSU’s written Hazard Communication Program to comply with state and federal regulations.

•  Each laboratory employee is expected to review the manual when they start work.

OHSU Laboratory Safety Manual •  Major topics covered in the

manual include: –  Introduction (Purpose, Scope

and Definitions) –  Hazardous Materials Program –  Standard Operating

Procedures for Working with Hazardous Materials

–  Control Measures (e.g., use of hoods, air sampling, etc.)

–  Plan Review

•  Appendices provide additional information on transportation and storage, glove performance data, chemical incompatibilities, etc.

•  Consult with your supervisor regarding any unique hazards specific to the work done in your lab.

Additional OHSU Training Resources

•  The OHSU Department of Environmental Health and Radiation Safety also provides several additional online training programs. You are required to take several Big Brain training classes, including General Safety and Laboratory Safety.

•  Additionally, your supervisor may recommend

you take other classes including: Back Safety; Bloodborne Pathogens; Radiation Safety; and Fire Safety & Evacuation.

Quiz Question 1 The OHSU Laboratory Safety Manual:

1. Satisfies OSHA requirements for a written Hazard Communication Program.

2. Discusses the Hazardous Materials Program.

3. Describes Standard Operating Procedures for working with Hazardous Materials.

4. Provides information on Control Measures.

5. All of the above.

Safety Data Sheets (SDSs)

SDSs What are Safety Data

Sheets (SDSs)? Answer: Chemical safety

sheets written to meet the requirements of the OSHA Hazard Communication and Lab Safety Standards. Prior to 2012 they were referred to as Material Safety Data Sheets (MSDSs).

SDS Contents 1.  Identification 2.  Hazard(s) 3.  Composition/Information on

ingredients 4.  First aid measures 5.  Fire fighting measures 6.  Accidental release measures 7.  Handling and storage 8.  Exposure control/personal

protection 9.  Physical and chemical properties 10. Stability and reactivity 11. Toxicological information 12. Ecological information 13. Disposal considerations 14. Transport information 15. Regulatory information 16. Other information

SDS Locations at Occupational Health Sciences

Where do you find SDSs? SDSs are available in the

individual Institute laboratories. Please ask your supervisor where the SDSs for your laboratory are maintained and become familiar with the location.

SDSs can also be found on the

internet by searching for the chemical name.

Quiz Question 2 What information can I find on an SDS?

1.  Recommendation on protective clothing and gloves.

2.  Occupational exposure limits.

3.  Toxicity information.

4.  All answers are correct.

Quiz Question 3 Where can I find SDSs?

1.  On the internet by searching for the chemical name (e.g. msds liquid nitrogen).

2.  Contact your supervisor.

3.  SDSs are maintained within each laboratory.

4.  All answers are correct.

Protective Clothing

Protective Gloves When are protective gloves needed? Answer: Use gloves when handling

– hot or cold materials –  radioactive chemicals – hazardous chemicals – Animals, animal tissues & fluids, and

infectious agents.

Types of Protective Gloves Latex gloves –use with animals and

animal tissues & fluids and non-toxic chemicals (please notify your supervisor if you are or suspect you may be sensitive to latex products).

Nitrile gloves -(usually blue or purple at

the Institute) – recommended for most chemicals used in the Institute’s labs, such as hydrocarbon solvents and chemicals used in histology.

Specialty gloves -(such as 4H highly

resistant laminated foil gloves) - recommended for extremely hazardous chemicals.

Check with your supervisor or the

Laboratory Safety Manual if you have any questions on glove choices.

Rules for Wearing Protective Gloves

•  Select the proper glove and change gloves frequently to avoid breakthrough and contamination. Do not reuse gloves. Always wash hands after using gloves for protection from hazardous products.

•  Always remove gloves when traveling into public areas

(stairs, elevators, restrooms, outdoors). •  Always remove gloves when using computer keyboards,

opening doors or pressing elevator buttons. •  Remember - take those gloves off in public areas! Be

sensitive to the perception of danger by members of the public when they see individuals using personal protective equipment.

Protective Clothing and Safety Glasses

What other Protective Equipment is required?

Protective clothing (lab coats,

aprons, etc.) - required whenever there is a hazard of contaminating the clothing or skin with hazardous materials.

Safety glasses – required whenever

there is a hazard of splashing material into the eyes. Always wear eye protection when working with chemicals presenting a significant eye exposure hazard (liquid nitrogen, phenol, osmium tetroxide, etc.)

Quiz Question 4 Which statements are false?

1.  Latex gloves are recommended for biohazards and non-toxic materials.

2.  Nitrile gloves are effective against most lab chemicals used in our labs.

3.  Nitrile gloves stop the penetration of all chemicals.

4.  Use safety glasses when there is a splash hazard.

Quiz Question 5 Which statements are true?

1.  Latex gloves are good protection against hydrocarbon solvents.

2.  You don’t need to use gloves or safety glasses with liquid nitrogen.

3.  Wear thermal gloves when removing items from a hot autoclave.

4.  Use latex gloves when embedding tissues for microscopy.

Lab Rules and Procedures

General Lab Rules and Procedures

•  Staff are encouraged to get

away from work stations and enjoy meals in the café, atrium or other spaces.

•  Eating or drinking is not allowed in non-shielded and designated personal spaces.

•  Chemicals and toxic materials are not allowed in designated personal spaces.

.

General Lab Rules and Procedures (continued)

•  Children under 14 years of age

are not allowed in Institute labs.

•  Professional behavior is required at all times (no running, horseplay, etc.).

•  Use personal protective equipment required for the task you are working on.

•  Do not carry open lab chemicals (open test tubes, uncovered beakers, etc.) into public spaces (elevators, restrooms, etc.).

Quiz Question 6 Which statement is false?

1.  You can have a covered drink in personal space areas.

2.  It is ok to eat at a lab bench.

3.  Do not carry open lab chemicals into public spaces.

4.  Always wear safety glasses when pouring liquid nitrogen.

Required Training/Health Evaluation for New Employees

All new lab research staff employees (permanent staff, summer employees, interns and volunteers) must complete the following requirements, in addition to this training:

1.  The OHSU Big Brain Laboratory Safety course.

2.  Health Insurance Portability and Accounting Act (HIPAA) Privacy Education.

3.  Responsible Conduct in Research (RCR) Education (see).

4.  Health Evaluation: TB test, immunization history, prescreening to determine if Hepatitis B vaccine is warranted.

5.  Animal Safety and Handling Training (if working with animals).

6.  Bloodborne Pathogens Training (if working with human tissues, blood, or other potentially infectious materials).

Access Big Brain Courses at: https://bigbrain.ohsu.edu/

Waste Disposal Rules This chart summarizes the management of general, medical, and hazardous wastes at OHSU.

Hazardous Waste Rules •  Specific rules on hazardous waste disposal are found

on the OHSU Safety Webpage. •  Do not drain dispose chemicals unless approved on

the OHSU Safety Webpage or in the Radioisotope License.

•  Talk to your supervisor for additional rules regarding radioisotopes, human tissues or body fluids.

•  Ask your lab safety committee representative if you have any questions about hazardous waste storage or disposal or safety.

Quiz Question 7 Which is true?

1.  OHSU has detailed rules for disposal of general, medical, and hazardous wastes.

2.  The OHSU safety website lists chemicals prohibited from drain disposal.

3.  All sharps must be disposed in sharps containers.

4.  All human and animal tissues and blood must go into red biohazard bags.

5.  All of the above.

Use of Animals •  The Department of Comparative Medicine oversees the

use of research animals at OHSU and provides training for new laboratory animal users. Where applicable, new staff must attend an animal handling training program prior to beginning work with animals.

•  Be aware that allergies to laboratory animals affect a sizeable percentage of animal users. Wear facemasks, gloves, and clean laboratory clothing, and wash hands after contact to prevent development of allergic reactions to animals.

•  Be sensitive to the perceptions of coworkers and visitors to the lab! Transport animals in closed containers and keep animal carcasses and excised tissues in a covered container during transportation. Use the freight elevator for these tasks – not the passenger elevators.

Quiz Question 8 Which is true?

1.  No training is required before handling research animals.

2.  People cannot develop allergies to lab animals.

3.  You should transport animals only on the freight elevator, not on the passenger elevators.

4.  None of the above.

Proper Hood Use •  Always work under a hood if

you are using volatile or hazardous chemicals or biohazardous materials.

•  Remember that the hood

sash must be lowered to provide maximum protection.

•  Always wear the required

protective equipment when working in a hood.

Understand the Types of Hoods Used at Occupational

Health Sciences

•  Chemical fume hoods – use when handling volatile & toxic chemicals.

Understand the Types of Hoods Used at the Occupational

Health Sciences •  Biosafety hoods –

use when handling biological materials with potential risk to workers. Always use a vented biosafety hood for work involving biologicals and toxic chemicals.

Understand the Types of Hoods Used at Occupational Health

Sciences •  Clean benches –

use when handling nonhazardous biological materials to protect the materials from contamination.

Quiz Question 9 Which is true?

1.  Chemical fume hoods are designed for exposure control with volatile and toxic chemicals.

2.  Biosafety hoods protect workers from exposure to biohazards.

3.  Vented biosafety hoods are appropriate for use with combined biohazard and chemical exposures.

4.  Clean benches only protect the product – not the worker at the hood.

5.  All of the above.

Emergency Information

Know Your Surroundings

•  Our labs and offices occupy approximately 30,000 square feet on floors 0, 1, 2, and 3 of the Richard Jones Hall/ Annex Building.

•  Each floor is served by a

passenger elevator and freight elevator. The building and freight elevators are locked between the hours of 6 p.m. and 7 a.m. and on weekends and holidays.

Building Map •  This figure illustrates the 2nd

floor of our space to help orient you to our space.

•  A public stairway (A) is located

at the southeast end of the building. Internal stairways (B & C) connect our spaces on floors 0–2 (west end of lab areas) and floors 2–3 (by the Administration Office). An emergency only exit stairway (D) serving all floors is located at the southwest corner of the building.

•  (For your reference – the

Administration Office in 2501 is at the northeast corner of the building)

OHSU Emergency Response Plan

•  OHSU has a well-developed Emergency Response Plan to deal with a broad range of emergency situations (medical emergencies/injuries, fires, hazardous materials spills, etc.)

•  Research units such as our

Institute are part of this plan. Detailed Emergency Resource Books have been prepared by OHSU Safety Department and are posted throughout our spaces.

Emergency Notifications and Phone Numbers

If you observe an emergency situation at work, immediately do the following:

•  Make sure your supervisor or other Oregon Institute of

Occupational Sciences administrator is notified.

•  If there is a medical emergency or other incident that requires immediate attention, dial Campus Security at 4-4444. (For non-emergency information, always dial 4-7744).

•  If you are able to do so safely, administer needed first aid, use a fire extinguisher, etc. as needed until help arrives.

Emergency Alarms •  The building fire alarm will sound in the event

of a fire or other emergency. Immediately leave your work area and exit via the stairs (see following sections for specific instructions).

•  Provide assistance as needed to help others

exit the building. •  Do not reenter the building until the alarm has

been silenced and the building is cleared.

Procedures for Injuries and Exposures

What to do if you are injured or exposed to a chemical: •  If there is chemical contamination, immediately wash

skin or eyes with water using eyewash or shower.

•  Contact your supervisor or other authority. •  Go to OHSU emergency room for serious injuries.

•  Seek assistance if needed (campus 4-4444).

•  Make sure you and your supervisor fill out an OHSU Incident Report Form within 24 hours of the incident (see your supervisor).

Emergency Information – Fire Exits & Rally Points

Fire drills/evacuation – fire & emergency alarms require that you leave the building.

The emergency rally point is in front of the building by the

marble head. If this area is unsafe due to the presence of emergency vehicles, fallen debris, etc., proceed to Mackenzie Hall by the fountain.

Emergency Exits for fire or other emergencies are located

at the southeast and southwest ends of the CROET building.

Do not use the elevators in an emergency situation.

Emergency Information – Main Fire Exit

•  Use the southeast exit stairway as your main fire/emergency exit leading up to the 3rd floor and out to the courtyard rally point.

Emergency Information – Back Fire Exit

•  You can also use the southwest exit stairway at the back of the building, but there is no reentry to the building from this stairway. This stairway exits at the bottom level.

Emergency Information – Fire Extinguishers

•  Fire Extinguishers are located on all floors in the lab corridors.

To Use a Fire Extinguisher: 1.  Pull the pin, 2.  Aim at the base of the fire, 3.  Squeeze the handles, 4.  Sweep the spray from side

to side until the fire is out.

Emergency Information – Safety Shower

•  Safety Showers are located on all floors in the lab corridors.

•  Pull the overhead

handle to activate the drench shower.

Emergency Information - Eyewash

•  Use the eyewash for at least 15 minutes if you splash a chemical into your eyes.

Automated External Defibrillator (AED)

•  An AED is located in the RJH atrium. AEDs can be used by any motivated bystander including Institute staff if a victim suffers sudden cardiac arrest.

Quiz Question 10 Which is false?

1.  When you exit the building in a fire situation, assemble by the marble head in front of building. If this area is obstructed, assemble by the fountain in front of Mackenzie Hall.

2.  Use eyewash for at least 15 minutes if you splash a hazardous chemical into your eye.

3.  It is ok to stay in the building when a fire alarm goes off – it might be a false alarm.

4.  The fire exit at the southwest end of the building does not allow reentry.

Safety Problems or Concerns

•  Thank you for completing this training segment. Make sure you turn in your score sheet to Janice Stewart.

•  If you notice a safety problem that concerns you, contact your supervisor or Dede Montgomery (montgomd@ohsu.edu; 494-4090). If desired, your name will not be revealed.